SPIRE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPIRE1
Entrez Gene ID	490562
Full Name	spire type actin nucleation factor 1
General protein information	Preferred Names spire type actin nucleation factor 1 Names protein spire homolog 1 spire homolog 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_547684.5	XP_547684.4	protein spire homolog 1
XM_022421645.1	XP_022277353.1	protein spire homolog 1 isoform X2
XM_022421646.1	XP_022277354.1	protein spire homolog 1 isoform X3
XM_547684.6	XP_547684.5	protein spire homolog 1 isoform X4
XM_022421648.1	XP_022277356.1	protein spire homolog 1 isoform X6
XM_022421649.1	XP_022277357.1	protein spire homolog 1 isoform X7
XM_022421644.1	XP_022277352.1	protein spire homolog 1 isoform X1
XM_022421647.1	XP_022277355.1	protein spire homolog 1 isoform X5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPIRE1	XP_547684.4
Gallus gallus (chicken)	SPIRE1	XP_004939777.1
Mus musculus (house mouse)	Spire1	NP_919336.1
Xenopus tropicalis (tropical clawed frog)	spire1	XP_002936125.2
Pan troglodytes (chimpanzee)	SPIRE1	XP_003315842.1
Rattus norvegicus (Norway rat)	Spire1	NP_001100851.1
Danio rerio (zebrafish)	spire1a	NP_001038312.2
Homo sapiens (human)	SPIRE1	NP_001122098.1
Bos taurus (cattle)	SPIRE1	XP_005224371.1

Gene Ontology

The following SPIRE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPIRE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe09989	XM_547684.5 Latest version!	Canis lupus familiaris spire-type actin nucleation factor 1 (SPIRE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe289899	XM_022421644.1 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe289900	XM_022421645.1 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe289901	XM_022421646.1 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe289902	XM_547684.6 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe289903	XM_022421647.1 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe289904	XM_022421648.1 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe289905	XM_022421649.1 Latest version!	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPIRE1 ( XM_547684.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547684.4 Canis lupus familiaris spire-type actin nucleation factor 1 (SPIRE1), mRNA.

Price & Availability↑

CloneID	OCe09989
Clone ID Related Accession (Same CDS sequence)	XM_547684.5
Accession Version	XM_547684.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein spire homolog 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726068.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547684.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAATA CAGTAGAAGC TGATGGGAGC AATGATGAAG GCTATGGGGC TGCCGATGAA 
GGTCTGGAAG ATGAAGAAGA TGAAAAGAGA AAAGTCTCAG CCATCCAATC ATACAGAGAT 
GTCATGAAGT TATGTGCTGC TCATCTTCCA ACTGAATCAG AGGCACCAAA TCATTATCAG 
GCAGTATGTC GTGCACTGTT TGCAGAAACA ATGGAGCTGC ATACATTTCT GACTAAGATT 
AAGAGTGCAA AGGAGAATCT TAAGAAGATT CAAGAAATGG AAAAGACTGA TGAATCTAAC 
ACAGATCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGAT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA ACTCAAGAAG GTCCAAGAGC GGCAGTACAA TCCTTTGCCC 
ATCGAATATC AACTCACCCC TTATGAGATG CTGATGGATG ACATTCGATC CAAAAGATAC 
ACTTTGCGCA AAGTAATGGT CAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAC 
GAAATCATCC TGGACTTCAT CAGATCAAGA CCCCCTTTAA AGCCGGTTGC AGCCAGAAAA 
CTGAAACCCA CTCCACCACG ACCACGGAGC CTCCATGAAA GAATATTAGA AGAAATCAAA 
GCAGAAAGAA AGCTACGGCC TGTCTCACCA GAGGAGATTA GACGCAGCAG ATTAGATGTA 
AATACTCCTG AATCTCCAAA GAACACTGTG GAATCTTCAA TGGTGAATGG AGGTTTCCCA 
TCACAAACAA AAGAAAATGG ATTGAGTGCT GCACAGCAGG TGCCTGTGCA GCGGAAGAAG 
CTCCTCAAAG CTCCAACTCT GGCTGAACTG GACAGCTCTG ACTCCGAGGA AGAAACTCTG 
CACAAGTCTA CCAGCAGCAG CAGTGTATCT CCCTCCTTCC TTGAAGATCC CTTAGCAGAG 
GCTGTGTCCA CAAGGAAGAA GCCTCCAAAA TTCTTGCCCA TATCATCAAC ACCCCAGCCA 
GAGAGACGAC AGCCACCCCA GAGACGACAT TCCATTGAAA AGGAAACACC CACTAATGTA 
AGACAGTTTC TGCCACCGTC CAAGCAGAGC TCCCGATCTC TTGTTCCTAG GATAACCAGT 
GTTTGGCCAA GGACGCCTTT CCGACCACTT TTTTCTACCA TACAAACAGC TTCTCTGCTC 
AGCTCTCATC CTTTTGAAGC AGCCATGTTT GGGGTAGCAG GGGCTATGTA TTATCTATGT 
GAGAGAGCTT TTACCAGCAG GTGGAAATCC TCAAAGGAGG AATTTTGCTA TCCAGTGGAA 
TGCCTAGCTC TTACTGTGGA GGAAGTGATG CATATTCGTC AGGTCCTGGT GAAGGCAGAA 
CTGGAAAAGT ACCAACAGTA TAAAGATGTC TACACCGCCT TGAAAAAAGG AAAGCTCTGC 
TTTTGTTGCC GAACCAGAAG ATTTTCCTTC TTCACCTGGT CTTATACCTG TCAGTTCTGT 
AAGAGGCCGG TGTGTTCACA GTGTTGCAAA AAGATGCGGC TGCCCTCCAA ACCATACTCC 
ACTCTTCCCA TCTTTTCATT GGGACCTTCT GCCTTGCAAA GAGGGGAAAG TTGTATGAGG 
GCAGAAAAGC CCACCACTGG CCACCATCGG CCACTCCGGA GCATTGCCAG GTTCTCCTCA 
AAATCTAAAT CTGTAGACAA ATCAGATGAA GAACTACAGT TTCCCAAAGA GCTGATGGAG 
GACTGGAGTA CTATGGAGGT GTGTGTAGAC TGCAAAAAGT TTATTTCGGA AATCATCAGT 
TCAAGCCGGC GCAGCCTGGT GTTGGCCAAC AAAAGAGCCC GATTAAAAAG GAAAACACAG 
TCTTTCTATA TGTCCTCACC AGGCCCCTCG GAATACTGCC CTTCAGAGAG GACTATCAAT 
GAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547684.4
CDS	100..2028
Translation	MANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLVPRITSVWPRTPFRPLFSTIQTASLLSSHPFEAAMFGVAGAMYYLCERAFTSRWKSSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_547684.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris spire-type actin nucleation factor 1 (SPIRE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547684.5

ORF Insert Sequence:

ATGGCCAATA CAGTAGAAGC TGATGGGAGC AATGATGAAG GCTATGGGGC TGCCGATGAA 
GGTCTGGAAG ATGAAGAAGA TGAAAAGAGA AAAGTCTCAG CCATCCAATC ATACAGAGAT 
GTCATGAAGT TATGTGCTGC TCATCTTCCA ACTGAATCAG AGGCACCAAA TCATTATCAG 
GCAGTATGTC GTGCACTGTT TGCAGAAACA ATGGAGCTGC ATACATTTCT GACTAAGATT 
AAGAGTGCAA AGGAGAATCT TAAGAAGATT CAAGAAATGG AAAAGACTGA TGAATCTAAC 
ACAGATCTGG AAGAGCTGAA AAATGCTGAC TGGGCTCGAT TCTGGGTACA GGTGATGAGG 
GATTTGCGGA ATGGAGTAAA ACTCAAGAAG GTCCAAGAGC GGCAGTACAA TCCTTTGCCC 
ATCGAATATC AACTCACCCC TTATGAGATG CTGATGGATG ACATTCGATC CAAAAGATAC 
ACTTTGCGCA AAGTAATGGT CAATGGTGAT ATTCCCCCTC GGTTAAAAAA AAGTGCTCAC 
GAAATCATCC TGGACTTCAT CAGATCAAGA CCCCCTTTAA AGCCGGTTGC AGCCAGAAAA 
CTGAAACCCA CTCCACCACG ACCACGGAGC CTCCATGAAA GAATATTAGA AGAAATCAAA 
GCAGAAAGAA AGCTACGGCC TGTCTCACCA GAGGAGATTA GACGCAGCAG ATTAGATGTA 
AATACTCCTG AATCTCCAAA GAACACTGTG GAATCTTCAA TGGTGAATGG AGGTTTCCCA 
TCACAAACAA AAGAAAATGG ATTGAGTGCT GCACAGCAGG TGCCTGTGCA GCGGAAGAAG 
CTCCTCAAAG CTCCAACTCT GGCTGAACTG GACAGCTCTG ACTCCGAGGA AGAAACTCTG 
CACAAGTCTA CCAGCAGCAG CAGTGTATCT CCCTCCTTCC TTGAAGATCC CTTAGCAGAG 
GCTGTGTCCA CAAGGAAGAA GCCTCCAAAA TTCTTGCCCA TATCATCAAC ACCCCAGCCA 
GAGAGACGAC AGCCACCCCA GAGACGACAT TCCATTGAAA AGGAAACACC CACTAATGTA 
AGACAGTTTC TGCCACCGTC CAAGCAGAGC TCCCGATCTC TTGTTCCTAG GATAACCAGT 
GTTTGGCCAA GGACGCCTTT CCGACCACTT TTTTCTACCA TACAAACAGC TTCTCTGCTC 
AGCTCTCATC CTTTTGAAGC AGCCATGTTT GGGGTAGCAG GGGCTATGTA TTATCTATGT 
GAGAGAGCTT TTACCAGCAG GTGGAAATCC TCAAAGGAGG AATTTTGCTA TCCAGTGGAA 
TGCCTAGCTC TTACTGTGGA GGAAGTGATG CATATTCGTC AGGTCCTGGT GAAGGCAGAA 
CTGGAAAAGT ACCAACAGTA TAAAGATGTC TACACCGCCT TGAAAAAAGG AAAGCTCTGC 
TTTTGTTGCC GAACCAGAAG ATTTTCCTTC TTCACCTGGT CTTATACCTG TCAGTTCTGT 
AAGAGGCCGG TGTGTTCACA GTGTTGCAAA AAGATGCGGC TGCCCTCCAA ACCATACTCC 
ACTCTTCCCA TCTTTTCATT GGGACCTTCT GCCTTGCAAA GAGGGGAAAG TTGTATGAGG 
GCAGAAAAGC CCACCACTGG CCACCATCGG CCACTCCGGA GCATTGCCAG GTTCTCCTCA 
AAATCTAAAT CTGTAGACAA ATCAGATGAA GAACTACAGT TTCCCAAAGA GCTGATGGAG 
GACTGGAGTA CTATGGAGGT GTGTGTAGAC TGCAAAAAGT TTATTTCGGA AATCATCAGT 
TCAAGCCGGC GCAGCCTGGT GTTGGCCAAC AAAAGAGCCC GATTAAAAAG GAAAACACAG 
TCTTTCTATA TGTCCTCACC AGGCCCCTCG GAATACTGCC CTTCAGAGAG GACTATCAAT 
GAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_022421644.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277352.1 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe289899
Clone ID Related Accession (Same CDS sequence)	XM_022421644.1
Accession Version	XM_022421644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGTTCC TAGGATAACC AGTGTTTGGC CAAGGACGCC TTTCCGACCA 
CTTTTTTCTA CCATACAAAC AGCTTCTCTG CTCAGCTCTC ATCCTTTTGA AGCAGCCATG 
TTTGGGGTAG CAGGGGCTAT GTATTATCTA TGTGAGAGAG CTTTTACCAG CAGGTGGAAA 
TCCTCAAAGG AGGAATTTTG CTATCCAGTG GAATGCCTAG CTCTTACTGT GGAGGAAGTG 
ATGCATATTC GTCAGGTCCT GGTGAAGGCA GAACTGGAAA AGTACCAACA GTATAAAGAT 
GTCTACACCG CCTTGAAAAA AGGAAAGCTC TGCTTTTGTT GCCGAACCAG AAGATTTTCC 
TTCTTCACCT GGTCTTATAC CTGTCAGTTC TGTAAGAGTC ACATCTATGG TACCATGCTG 
AGTAACACTT CTGATTTTGT ATACAGGCCG GTGTGTTCAC AGTGTTGCAA AAAGATGCGG 
CTGCCCTCCA AACCATACTC CACTCTTCCC ATCTTTTCAT TGGGACCTTC TGCCTTGCAA 
AGAGGGGAAA GTTGTATGAG GGCAGAAAAG CCCACCACTG GCCACCATCG GCCACTCCGG 
AGCATTGCCA GGTTCTCCTC AAAATCTAAA TCTGTAGACA AATCAGATGA AGAACTACAG 
TTTCCCAAAG AGCTGATGGA GGACTGGAGT ACTATGGAGG TGTGTGTAGA CTGCAAAAAG 
TTTATTTCGG AAATCATCAG TTCAAGCCGG CGCAGCCTGG TGTTGGCCAA CAAAAGAGCC 
CGATTAAAAA GGAAAACACA GTCTTTCTAT ATGTCCTCAC CAGGCCCCTC GGAATACTGC 
CCTTCAGAGA GGACTATCAA TGAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277352.1
CDS	101..2290
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAMRPLSMSYSFDLSDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLVPRITSVWPRTPFRPLFSTIQTASLLSSHPFEAAMFGVAGAMYYLCERAFTSRWKSSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKSHIYGTMLSNTSDFVYRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_022421644.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421644.1

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGTTCC TAGGATAACC AGTGTTTGGC CAAGGACGCC TTTCCGACCA 
CTTTTTTCTA CCATACAAAC AGCTTCTCTG CTCAGCTCTC ATCCTTTTGA AGCAGCCATG 
TTTGGGGTAG CAGGGGCTAT GTATTATCTA TGTGAGAGAG CTTTTACCAG CAGGTGGAAA 
TCCTCAAAGG AGGAATTTTG CTATCCAGTG GAATGCCTAG CTCTTACTGT GGAGGAAGTG 
ATGCATATTC GTCAGGTCCT GGTGAAGGCA GAACTGGAAA AGTACCAACA GTATAAAGAT 
GTCTACACCG CCTTGAAAAA AGGAAAGCTC TGCTTTTGTT GCCGAACCAG AAGATTTTCC 
TTCTTCACCT GGTCTTATAC CTGTCAGTTC TGTAAGAGTC ACATCTATGG TACCATGCTG 
AGTAACACTT CTGATTTTGT ATACAGGCCG GTGTGTTCAC AGTGTTGCAA AAAGATGCGG 
CTGCCCTCCA AACCATACTC CACTCTTCCC ATCTTTTCAT TGGGACCTTC TGCCTTGCAA 
AGAGGGGAAA GTTGTATGAG GGCAGAAAAG CCCACCACTG GCCACCATCG GCCACTCCGG 
AGCATTGCCA GGTTCTCCTC AAAATCTAAA TCTGTAGACA AATCAGATGA AGAACTACAG 
TTTCCCAAAG AGCTGATGGA GGACTGGAGT ACTATGGAGG TGTGTGTAGA CTGCAAAAAG 
TTTATTTCGG AAATCATCAG TTCAAGCCGG CGCAGCCTGG TGTTGGCCAA CAAAAGAGCC 
CGATTAAAAA GGAAAACACA GTCTTTCTAT ATGTCCTCAC CAGGCCCCTC GGAATACTGC 
CCTTCAGAGA GGACTATCAA TGAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_022421645.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277353.1 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe289900
Clone ID Related Accession (Same CDS sequence)	XM_022421645.1
Accession Version	XM_022421645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGATGT AAATACTCCT 
GAATCTCCAA AGAACACTGT GGAATCTTCA ATGGTGAATG GAGGTTTCCC ATCACAAACA 
AAAGAAAATG GATTGAGTGC TGCACAGCAG GTGCCTGTGC AGCGGAAGAA GCTCCTCAAA 
GCTCCAACTC TGGCTGAACT GGACAGCTCT GACTCCGAGG AAGAAACTCT GCACAAGTCT 
ACCAGCAGCA GCAGTGTATC TCCCTCCTTC CTTGAAGATC CCTTAGCAGA GGCTGTGTCC 
ACAAGGAAGA AGCCTCCAAA ATTCTTGCCC ATATCATCAA CACCCCAGCC AGAGAGACGA 
CAGCCACCCC AGAGACGACA TTCCATTGAA AAGGAAACAC CCACTAATGT AAGACAGTTT 
CTGCCACCGT CCAAGCAGAG CTCCCGATCT CTTGTTCCTA GGATAACCAG TGTTTGGCCA 
AGGACGCCTT TCCGACCACT TTTTTCTACC ATACAAACAG CTTCTCTGCT CAGCTCTCAT 
CCTTTTGAAG CAGCCATGTT TGGGGTAGCA GGGGCTATGT ATTATCTATG TGAGAGAGCT 
TTTACCAGCA GGTGGAAATC CTCAAAGGAG GAATTTTGCT ATCCAGTGGA ATGCCTAGCT 
CTTACTGTGG AGGAAGTGAT GCATATTCGT CAGGTCCTGG TGAAGGCAGA ACTGGAAAAG 
TACCAACAGT ATAAAGATGT CTACACCGCC TTGAAAAAAG GAAAGCTCTG CTTTTGTTGC 
CGAACCAGAA GATTTTCCTT CTTCACCTGG TCTTATACCT GTCAGTTCTG TAAGAGTCAC 
ATCTATGGTA CCATGCTGAG TAACACTTCT GATTTTGTAT ACAGGCCGGT GTGTTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAA CCATACTCCA CTCTTCCCAT CTTTTCATTG 
GGACCTTCTG CCTTGCAAAG AGGGGAAAGT TGTATGAGGG CAGAAAAGCC CACCACTGGC 
CACCATCGGC CACTCCGGAG CATTGCCAGG TTCTCCTCAA AATCTAAATC TGTAGACAAA 
TCAGATGAAG AACTACAGTT TCCCAAAGAG CTGATGGAGG ACTGGAGTAC TATGGAGGTG 
TGTGTAGACT GCAAAAAGTT TATTTCGGAA ATCATCAGTT CAAGCCGGCG CAGCCTGGTG 
TTGGCCAACA AAAGAGCCCG ATTAAAAAGG AAAACACAGT CTTTCTATAT GTCCTCACCA 
GGCCCCTCGG AATACTGCCC TTCAGAGAGG ACTATCAATG AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277353.1
CDS	101..2248
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLVPRITSVWPRTPFRPLFSTIQTASLLSSHPFEAAMFGVAGAMYYLCERAFTSRWKSSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKSHIYGTMLSNTSDFVYRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_022421645.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421645.1

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGATGT AAATACTCCT 
GAATCTCCAA AGAACACTGT GGAATCTTCA ATGGTGAATG GAGGTTTCCC ATCACAAACA 
AAAGAAAATG GATTGAGTGC TGCACAGCAG GTGCCTGTGC AGCGGAAGAA GCTCCTCAAA 
GCTCCAACTC TGGCTGAACT GGACAGCTCT GACTCCGAGG AAGAAACTCT GCACAAGTCT 
ACCAGCAGCA GCAGTGTATC TCCCTCCTTC CTTGAAGATC CCTTAGCAGA GGCTGTGTCC 
ACAAGGAAGA AGCCTCCAAA ATTCTTGCCC ATATCATCAA CACCCCAGCC AGAGAGACGA 
CAGCCACCCC AGAGACGACA TTCCATTGAA AAGGAAACAC CCACTAATGT AAGACAGTTT 
CTGCCACCGT CCAAGCAGAG CTCCCGATCT CTTGTTCCTA GGATAACCAG TGTTTGGCCA 
AGGACGCCTT TCCGACCACT TTTTTCTACC ATACAAACAG CTTCTCTGCT CAGCTCTCAT 
CCTTTTGAAG CAGCCATGTT TGGGGTAGCA GGGGCTATGT ATTATCTATG TGAGAGAGCT 
TTTACCAGCA GGTGGAAATC CTCAAAGGAG GAATTTTGCT ATCCAGTGGA ATGCCTAGCT 
CTTACTGTGG AGGAAGTGAT GCATATTCGT CAGGTCCTGG TGAAGGCAGA ACTGGAAAAG 
TACCAACAGT ATAAAGATGT CTACACCGCC TTGAAAAAAG GAAAGCTCTG CTTTTGTTGC 
CGAACCAGAA GATTTTCCTT CTTCACCTGG TCTTATACCT GTCAGTTCTG TAAGAGTCAC 
ATCTATGGTA CCATGCTGAG TAACACTTCT GATTTTGTAT ACAGGCCGGT GTGTTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAA CCATACTCCA CTCTTCCCAT CTTTTCATTG 
GGACCTTCTG CCTTGCAAAG AGGGGAAAGT TGTATGAGGG CAGAAAAGCC CACCACTGGC 
CACCATCGGC CACTCCGGAG CATTGCCAGG TTCTCCTCAA AATCTAAATC TGTAGACAAA 
TCAGATGAAG AACTACAGTT TCCCAAAGAG CTGATGGAGG ACTGGAGTAC TATGGAGGTG 
TGTGTAGACT GCAAAAAGTT TATTTCGGAA ATCATCAGTT CAAGCCGGCG CAGCCTGGTG 
TTGGCCAACA AAAGAGCCCG ATTAAAAAGG AAAACACAGT CTTTCTATAT GTCCTCACCA 
GGCCCCTCGG AATACTGCCC TTCAGAGAGG ACTATCAATG AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_022421646.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277354.1 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe289901
Clone ID Related Accession (Same CDS sequence)	XM_022421646.1
Accession Version	XM_022421646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGTTCC TAGGATAACC AGTGTTTGGC CAAGGACGCC TTTCCGACCA 
CTTTTTTCTA CCATACAAAC AGCTTCTCTG CTCAGCTCTC ATCCTTTTGA AGCAGCCATG 
TTTGGGGTAG CAGGGGCTAT GTATTATCTA TGTGAGAGAG CTTTTACCAG CAGGTGGAAA 
TCCTCAAAGG AGGAATTTTG CTATCCAGTG GAATGCCTAG CTCTTACTGT GGAGGAAGTG 
ATGCATATTC GTCAGGTCCT GGTGAAGGCA GAACTGGAAA AGTACCAACA GTATAAAGAT 
GTCTACACCG CCTTGAAAAA AGGAAAGCTC TGCTTTTGTT GCCGAACCAG AAGATTTTCC 
TTCTTCACCT GGTCTTATAC CTGTCAGTTC TGTAAGAGGC CGGTGTGTTC ACAGTGTTGC 
AAAAAGATGC GGCTGCCCTC CAAACCATAC TCCACTCTTC CCATCTTTTC ATTGGGACCT 
TCTGCCTTGC AAAGAGGGGA AAGTTGTATG AGGGCAGAAA AGCCCACCAC TGGCCACCAT 
CGGCCACTCC GGAGCATTGC CAGGTTCTCC TCAAAATCTA AATCTGTAGA CAAATCAGAT 
GAAGAACTAC AGTTTCCCAA AGAGCTGATG GAGGACTGGA GTACTATGGA GGTGTGTGTA 
GACTGCAAAA AGTTTATTTC GGAAATCATC AGTTCAAGCC GGCGCAGCCT GGTGTTGGCC 
AACAAAAGAG CCCGATTAAA AAGGAAAACA CAGTCTTTCT ATATGTCCTC ACCAGGCCCC 
TCGGAATACT GCCCTTCAGA GAGGACTATC AATGAAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277354.1
CDS	101..2242
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAMRPLSMSYSFDLSDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLVPRITSVWPRTPFRPLFSTIQTASLLSSHPFEAAMFGVAGAMYYLCERAFTSRWKSSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_022421646.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421646.1

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGTTCC TAGGATAACC AGTGTTTGGC CAAGGACGCC TTTCCGACCA 
CTTTTTTCTA CCATACAAAC AGCTTCTCTG CTCAGCTCTC ATCCTTTTGA AGCAGCCATG 
TTTGGGGTAG CAGGGGCTAT GTATTATCTA TGTGAGAGAG CTTTTACCAG CAGGTGGAAA 
TCCTCAAAGG AGGAATTTTG CTATCCAGTG GAATGCCTAG CTCTTACTGT GGAGGAAGTG 
ATGCATATTC GTCAGGTCCT GGTGAAGGCA GAACTGGAAA AGTACCAACA GTATAAAGAT 
GTCTACACCG CCTTGAAAAA AGGAAAGCTC TGCTTTTGTT GCCGAACCAG AAGATTTTCC 
TTCTTCACCT GGTCTTATAC CTGTCAGTTC TGTAAGAGGC CGGTGTGTTC ACAGTGTTGC 
AAAAAGATGC GGCTGCCCTC CAAACCATAC TCCACTCTTC CCATCTTTTC ATTGGGACCT 
TCTGCCTTGC AAAGAGGGGA AAGTTGTATG AGGGCAGAAA AGCCCACCAC TGGCCACCAT 
CGGCCACTCC GGAGCATTGC CAGGTTCTCC TCAAAATCTA AATCTGTAGA CAAATCAGAT 
GAAGAACTAC AGTTTCCCAA AGAGCTGATG GAGGACTGGA GTACTATGGA GGTGTGTGTA 
GACTGCAAAA AGTTTATTTC GGAAATCATC AGTTCAAGCC GGCGCAGCCT GGTGTTGGCC 
AACAAAAGAG CCCGATTAAA AAGGAAAACA CAGTCTTTCT ATATGTCCTC ACCAGGCCCC 
TCGGAATACT GCCCTTCAGA GAGGACTATC AATGAAATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_547684.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547684.5 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe289902
Clone ID Related Accession (Same CDS sequence)	XM_547684.6
Accession Version	XM_547684.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_547684.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGATGT AAATACTCCT 
GAATCTCCAA AGAACACTGT GGAATCTTCA ATGGTGAATG GAGGTTTCCC ATCACAAACA 
AAAGAAAATG GATTGAGTGC TGCACAGCAG GTGCCTGTGC AGCGGAAGAA GCTCCTCAAA 
GCTCCAACTC TGGCTGAACT GGACAGCTCT GACTCCGAGG AAGAAACTCT GCACAAGTCT 
ACCAGCAGCA GCAGTGTATC TCCCTCCTTC CTTGAAGATC CCTTAGCAGA GGCTGTGTCC 
ACAAGGAAGA AGCCTCCAAA ATTCTTGCCC ATATCATCAA CACCCCAGCC AGAGAGACGA 
CAGCCACCCC AGAGACGACA TTCCATTGAA AAGGAAACAC CCACTAATGT AAGACAGTTT 
CTGCCACCGT CCAAGCAGAG CTCCCGATCT CTTGTTCCTA GGATAACCAG TGTTTGGCCA 
AGGACGCCTT TCCGACCACT TTTTTCTACC ATACAAACAG CTTCTCTGCT CAGCTCTCAT 
CCTTTTGAAG CAGCCATGTT TGGGGTAGCA GGGGCTATGT ATTATCTATG TGAGAGAGCT 
TTTACCAGCA GGTGGAAATC CTCAAAGGAG GAATTTTGCT ATCCAGTGGA ATGCCTAGCT 
CTTACTGTGG AGGAAGTGAT GCATATTCGT CAGGTCCTGG TGAAGGCAGA ACTGGAAAAG 
TACCAACAGT ATAAAGATGT CTACACCGCC TTGAAAAAAG GAAAGCTCTG CTTTTGTTGC 
CGAACCAGAA GATTTTCCTT CTTCACCTGG TCTTATACCT GTCAGTTCTG TAAGAGGCCG 
GTGTGTTCAC AGTGTTGCAA AAAGATGCGG CTGCCCTCCA AACCATACTC CACTCTTCCC 
ATCTTTTCAT TGGGACCTTC TGCCTTGCAA AGAGGGGAAA GTTGTATGAG GGCAGAAAAG 
CCCACCACTG GCCACCATCG GCCACTCCGG AGCATTGCCA GGTTCTCCTC AAAATCTAAA 
TCTGTAGACA AATCAGATGA AGAACTACAG TTTCCCAAAG AGCTGATGGA GGACTGGAGT 
ACTATGGAGG TGTGTGTAGA CTGCAAAAAG TTTATTTCGG AAATCATCAG TTCAAGCCGG 
CGCAGCCTGG TGTTGGCCAA CAAAAGAGCC CGATTAAAAA GGAAAACACA GTCTTTCTAT 
ATGTCCTCAC CAGGCCCCTC GGAATACTGC CCTTCAGAGA GGACTATCAA TGAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547684.5
CDS	101..2200
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLVPRITSVWPRTPFRPLFSTIQTASLLSSHPFEAAMFGVAGAMYYLCERAFTSRWKSSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_547684.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547684.6

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGATGT AAATACTCCT 
GAATCTCCAA AGAACACTGT GGAATCTTCA ATGGTGAATG GAGGTTTCCC ATCACAAACA 
AAAGAAAATG GATTGAGTGC TGCACAGCAG GTGCCTGTGC AGCGGAAGAA GCTCCTCAAA 
GCTCCAACTC TGGCTGAACT GGACAGCTCT GACTCCGAGG AAGAAACTCT GCACAAGTCT 
ACCAGCAGCA GCAGTGTATC TCCCTCCTTC CTTGAAGATC CCTTAGCAGA GGCTGTGTCC 
ACAAGGAAGA AGCCTCCAAA ATTCTTGCCC ATATCATCAA CACCCCAGCC AGAGAGACGA 
CAGCCACCCC AGAGACGACA TTCCATTGAA AAGGAAACAC CCACTAATGT AAGACAGTTT 
CTGCCACCGT CCAAGCAGAG CTCCCGATCT CTTGTTCCTA GGATAACCAG TGTTTGGCCA 
AGGACGCCTT TCCGACCACT TTTTTCTACC ATACAAACAG CTTCTCTGCT CAGCTCTCAT 
CCTTTTGAAG CAGCCATGTT TGGGGTAGCA GGGGCTATGT ATTATCTATG TGAGAGAGCT 
TTTACCAGCA GGTGGAAATC CTCAAAGGAG GAATTTTGCT ATCCAGTGGA ATGCCTAGCT 
CTTACTGTGG AGGAAGTGAT GCATATTCGT CAGGTCCTGG TGAAGGCAGA ACTGGAAAAG 
TACCAACAGT ATAAAGATGT CTACACCGCC TTGAAAAAAG GAAAGCTCTG CTTTTGTTGC 
CGAACCAGAA GATTTTCCTT CTTCACCTGG TCTTATACCT GTCAGTTCTG TAAGAGGCCG 
GTGTGTTCAC AGTGTTGCAA AAAGATGCGG CTGCCCTCCA AACCATACTC CACTCTTCCC 
ATCTTTTCAT TGGGACCTTC TGCCTTGCAA AGAGGGGAAA GTTGTATGAG GGCAGAAAAG 
CCCACCACTG GCCACCATCG GCCACTCCGG AGCATTGCCA GGTTCTCCTC AAAATCTAAA 
TCTGTAGACA AATCAGATGA AGAACTACAG TTTCCCAAAG AGCTGATGGA GGACTGGAGT 
ACTATGGAGG TGTGTGTAGA CTGCAAAAAG TTTATTTCGG AAATCATCAG TTCAAGCCGG 
CGCAGCCTGG TGTTGGCCAA CAAAAGAGCC CGATTAAAAA GGAAAACACA GTCTTTCTAT 
ATGTCCTCAC CAGGCCCCTC GGAATACTGC CCTTCAGAGA GGACTATCAA TGAAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_022421647.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277355.1 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe289903
Clone ID Related Accession (Same CDS sequence)	XM_022421647.1
Accession Version	XM_022421647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGAGGA ATTTTGCTAT CCAGTGGAAT GCCTAGCTCT TACTGTGGAG 
GAAGTGATGC ATATTCGTCA GGTCCTGGTG AAGGCAGAAC TGGAAAAGTA CCAACAGTAT 
AAAGATGTCT ACACCGCCTT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGA 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGTCACAT CTATGGTACC 
ATGCTGAGTA ACACTTCTGA TTTTGTATAC AGGCCGGTGT GTTCACAGTG TTGCAAAAAG 
ATGCGGCTGC CCTCCAAACC ATACTCCACT CTTCCCATCT TTTCATTGGG ACCTTCTGCC 
TTGCAAAGAG GGGAAAGTTG TATGAGGGCA GAAAAGCCCA CCACTGGCCA CCATCGGCCA 
CTCCGGAGCA TTGCCAGGTT CTCCTCAAAA TCTAAATCTG TAGACAAATC AGATGAAGAA 
CTACAGTTTC CCAAAGAGCT GATGGAGGAC TGGAGTACTA TGGAGGTGTG TGTAGACTGC 
AAAAAGTTTA TTTCGGAAAT CATCAGTTCA AGCCGGCGCA GCCTGGTGTT GGCCAACAAA 
AGAGCCCGAT TAAAAAGGAA AACACAGTCT TTCTATATGT CCTCACCAGG CCCCTCGGAA 
TACTGCCCTT CAGAGAGGAC TATCAATGAA ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277355.1
CDS	101..2116
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAMRPLSMSYSFDLSDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKSHIYGTMLSNTSDFVYRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_022421647.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421647.1

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGAGGA ATTTTGCTAT CCAGTGGAAT GCCTAGCTCT TACTGTGGAG 
GAAGTGATGC ATATTCGTCA GGTCCTGGTG AAGGCAGAAC TGGAAAAGTA CCAACAGTAT 
AAAGATGTCT ACACCGCCTT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGA 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGTCACAT CTATGGTACC 
ATGCTGAGTA ACACTTCTGA TTTTGTATAC AGGCCGGTGT GTTCACAGTG TTGCAAAAAG 
ATGCGGCTGC CCTCCAAACC ATACTCCACT CTTCCCATCT TTTCATTGGG ACCTTCTGCC 
TTGCAAAGAG GGGAAAGTTG TATGAGGGCA GAAAAGCCCA CCACTGGCCA CCATCGGCCA 
CTCCGGAGCA TTGCCAGGTT CTCCTCAAAA TCTAAATCTG TAGACAAATC AGATGAAGAA 
CTACAGTTTC CCAAAGAGCT GATGGAGGAC TGGAGTACTA TGGAGGTGTG TGTAGACTGC 
AAAAAGTTTA TTTCGGAAAT CATCAGTTCA AGCCGGCGCA GCCTGGTGTT GGCCAACAAA 
AGAGCCCGAT TAAAAAGGAA AACACAGTCT TTCTATATGT CCTCACCAGG CCCCTCGGAA 
TACTGCCCTT CAGAGAGGAC TATCAATGAA ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_022421648.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277356.1 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe289904
Clone ID Related Accession (Same CDS sequence)	XM_022421648.1
Accession Version	XM_022421648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGAGGA ATTTTGCTAT CCAGTGGAAT GCCTAGCTCT TACTGTGGAG 
GAAGTGATGC ATATTCGTCA GGTCCTGGTG AAGGCAGAAC TGGAAAAGTA CCAACAGTAT 
AAAGATGTCT ACACCGCCTT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGA 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGTTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAA CCATACTCCA CTCTTCCCAT CTTTTCATTG 
GGACCTTCTG CCTTGCAAAG AGGGGAAAGT TGTATGAGGG CAGAAAAGCC CACCACTGGC 
CACCATCGGC CACTCCGGAG CATTGCCAGG TTCTCCTCAA AATCTAAATC TGTAGACAAA 
TCAGATGAAG AACTACAGTT TCCCAAAGAG CTGATGGAGG ACTGGAGTAC TATGGAGGTG 
TGTGTAGACT GCAAAAAGTT TATTTCGGAA ATCATCAGTT CAAGCCGGCG CAGCCTGGTG 
TTGGCCAACA AAAGAGCCCG ATTAAAAAGG AAAACACAGT CTTTCTATAT GTCCTCACCA 
GGCCCCTCGG AATACTGCCC TTCAGAGAGG ACTATCAATG AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277356.1
CDS	101..2068
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAMRPLSMSYSFDLSDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_022421648.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421648.1

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGCAAT GCGGCCACTT 
AGCATGTCTT ACAGTTTTGA CTTGTCAGAT GTAAATACTC CTGAATCTCC AAAGAACACT 
GTGGAATCTT CAATGGTGAA TGGAGGTTTC CCATCACAAA CAAAAGAAAA TGGATTGAGT 
GCTGCACAGC AGGTGCCTGT GCAGCGGAAG AAGCTCCTCA AAGCTCCAAC TCTGGCTGAA 
CTGGACAGCT CTGACTCCGA GGAAGAAACT CTGCACAAGT CTACCAGCAG CAGCAGTGTA 
TCTCCCTCCT TCCTTGAAGA TCCCTTAGCA GAGGCTGTGT CCACAAGGAA GAAGCCTCCA 
AAATTCTTGC CCATATCATC AACACCCCAG CCAGAGAGAC GACAGCCACC CCAGAGACGA 
CATTCCATTG AAAAGGAAAC ACCCACTAAT GTAAGACAGT TTCTGCCACC GTCCAAGCAG 
AGCTCCCGAT CTCTTGAGGA ATTTTGCTAT CCAGTGGAAT GCCTAGCTCT TACTGTGGAG 
GAAGTGATGC ATATTCGTCA GGTCCTGGTG AAGGCAGAAC TGGAAAAGTA CCAACAGTAT 
AAAGATGTCT ACACCGCCTT GAAAAAAGGA AAGCTCTGCT TTTGTTGCCG AACCAGAAGA 
TTTTCCTTCT TCACCTGGTC TTATACCTGT CAGTTCTGTA AGAGGCCGGT GTGTTCACAG 
TGTTGCAAAA AGATGCGGCT GCCCTCCAAA CCATACTCCA CTCTTCCCAT CTTTTCATTG 
GGACCTTCTG CCTTGCAAAG AGGGGAAAGT TGTATGAGGG CAGAAAAGCC CACCACTGGC 
CACCATCGGC CACTCCGGAG CATTGCCAGG TTCTCCTCAA AATCTAAATC TGTAGACAAA 
TCAGATGAAG AACTACAGTT TCCCAAAGAG CTGATGGAGG ACTGGAGTAC TATGGAGGTG 
TGTGTAGACT GCAAAAAGTT TATTTCGGAA ATCATCAGTT CAAGCCGGCG CAGCCTGGTG 
TTGGCCAACA AAAGAGCCCG ATTAAAAAGG AAAACACAGT CTTTCTATAT GTCCTCACCA 
GGCCCCTCGG AATACTGCCC TTCAGAGAGG ACTATCAATG AAATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPIRE1 ( XM_022421649.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277357.1 Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe289905
Clone ID Related Accession (Same CDS sequence)	XM_022421649.1
Accession Version	XM_022421649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein spire homolog 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGATGT AAATACTCCT 
GAATCTCCAA AGAACACTGT GGAATCTTCA ATGGTGAATG GAGGTTTCCC ATCACAAACA 
AAAGAAAATG GATTGAGTGC TGCACAGCAG GTGCCTGTGC AGCGGAAGAA GCTCCTCAAA 
GCTCCAACTC TGGCTGAACT GGACAGCTCT GACTCCGAGG AAGAAACTCT GCACAAGTCT 
ACCAGCAGCA GCAGTGTATC TCCCTCCTTC CTTGAAGATC CCTTAGCAGA GGCTGTGTCC 
ACAAGGAAGA AGCCTCCAAA ATTCTTGCCC ATATCATCAA CACCCCAGCC AGAGAGACGA 
CAGCCACCCC AGAGACGACA TTCCATTGAA AAGGAAACAC CCACTAATGT AAGACAGTTT 
CTGCCACCGT CCAAGCAGAG CTCCCGATCT CTTGAGGAAT TTTGCTATCC AGTGGAATGC 
CTAGCTCTTA CTGTGGAGGA AGTGATGCAT ATTCGTCAGG TCCTGGTGAA GGCAGAACTG 
GAAAAGTACC AACAGTATAA AGATGTCTAC ACCGCCTTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGATT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GTTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAACC ATACTCCACT 
CTTCCCATCT TTTCATTGGG ACCTTCTGCC TTGCAAAGAG GGGAAAGTTG TATGAGGGCA 
GAAAAGCCCA CCACTGGCCA CCATCGGCCA CTCCGGAGCA TTGCCAGGTT CTCCTCAAAA 
TCTAAATCTG TAGACAAATC AGATGAAGAA CTACAGTTTC CCAAAGAGCT GATGGAGGAC 
TGGAGTACTA TGGAGGTGTG TGTAGACTGC AAAAAGTTTA TTTCGGAAAT CATCAGTTCA 
AGCCGGCGCA GCCTGGTGTT GGCCAACAAA AGAGCCCGAT TAAAAAGGAA AACACAGTCT 
TTCTATATGT CCTCACCAGG CCCCTCGGAA TACTGCCCTT CAGAGAGGAC TATCAATGAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277357.1
CDS	101..2026
Translation	MKYIYSAKMISKLGYSHCTETEVIESLGIVIYKALDYGLKENEERELSPPLEQLIDHMANTVEADGSNDEGYGAADEGLEDEEDEKRKVSAIQSYRDVMKLCAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKTDESNTDLEELKNADWARFWVQVMRDLRNGVKLKKVQERQYNPLPIEYQLTPYEMLMDDIRSKRYTLRKVMVNGDIPPRLKKSAHEIILDFIRSRPPLKPVAARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVNTPESPKNTVESSMVNGGFPSQTKENGLSAAQQVPVQRKKLLKAPTLAELDSSDSEEETLHKSTSSSSVSPSFLEDPLAEAVSTRKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSKQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCMRAEKPTTGHHRPLRSIARFSSKSKSVDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_022421649.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris spire type actin nucleation factor 1 (SPIRE1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421649.1

ORF Insert Sequence:

ATGAAGTACA TTTATTCTGC AAAAATGATA AGTAAATTGG GATATTCACA TTGTACAGAA 
ACAGAGGTTA TTGAATCTCT GGGAATTGTT ATTTATAAAG CACTGGACTA TGGTTTGAAG 
GAAAATGAAG AAAGGGAGTT AAGCCCCCCA CTGGAGCAGC TAATTGATCA CATGGCCAAT 
ACAGTAGAAG CTGATGGGAG CAATGATGAA GGCTATGGGG CTGCCGATGA AGGTCTGGAA 
GATGAAGAAG ATGAAAAGAG AAAAGTCTCA GCCATCCAAT CATACAGAGA TGTCATGAAG 
TTATGTGCTG CTCATCTTCC AACTGAATCA GAGGCACCAA ATCATTATCA GGCAGTATGT 
CGTGCACTGT TTGCAGAAAC AATGGAGCTG CATACATTTC TGACTAAGAT TAAGAGTGCA 
AAGGAGAATC TTAAGAAGAT TCAAGAAATG GAAAAGACTG ATGAATCTAA CACAGATCTG 
GAAGAGCTGA AAAATGCTGA CTGGGCTCGA TTCTGGGTAC AGGTGATGAG GGATTTGCGG 
AATGGAGTAA AACTCAAGAA GGTCCAAGAG CGGCAGTACA ATCCTTTGCC CATCGAATAT 
CAACTCACCC CTTATGAGAT GCTGATGGAT GACATTCGAT CCAAAAGATA CACTTTGCGC 
AAAGTAATGG TCAATGGTGA TATTCCCCCT CGGTTAAAAA AAAGTGCTCA CGAAATCATC 
CTGGACTTCA TCAGATCAAG ACCCCCTTTA AAGCCGGTTG CAGCCAGAAA ACTGAAACCC 
ACTCCACCAC GACCACGGAG CCTCCATGAA AGAATATTAG AAGAAATCAA AGCAGAAAGA 
AAGCTACGGC CTGTCTCACC AGAGGAGATT AGACGCAGCA GATTAGATGT AAATACTCCT 
GAATCTCCAA AGAACACTGT GGAATCTTCA ATGGTGAATG GAGGTTTCCC ATCACAAACA 
AAAGAAAATG GATTGAGTGC TGCACAGCAG GTGCCTGTGC AGCGGAAGAA GCTCCTCAAA 
GCTCCAACTC TGGCTGAACT GGACAGCTCT GACTCCGAGG AAGAAACTCT GCACAAGTCT 
ACCAGCAGCA GCAGTGTATC TCCCTCCTTC CTTGAAGATC CCTTAGCAGA GGCTGTGTCC 
ACAAGGAAGA AGCCTCCAAA ATTCTTGCCC ATATCATCAA CACCCCAGCC AGAGAGACGA 
CAGCCACCCC AGAGACGACA TTCCATTGAA AAGGAAACAC CCACTAATGT AAGACAGTTT 
CTGCCACCGT CCAAGCAGAG CTCCCGATCT CTTGAGGAAT TTTGCTATCC AGTGGAATGC 
CTAGCTCTTA CTGTGGAGGA AGTGATGCAT ATTCGTCAGG TCCTGGTGAA GGCAGAACTG 
GAAAAGTACC AACAGTATAA AGATGTCTAC ACCGCCTTGA AAAAAGGAAA GCTCTGCTTT 
TGTTGCCGAA CCAGAAGATT TTCCTTCTTC ACCTGGTCTT ATACCTGTCA GTTCTGTAAG 
AGGCCGGTGT GTTCACAGTG TTGCAAAAAG ATGCGGCTGC CCTCCAAACC ATACTCCACT 
CTTCCCATCT TTTCATTGGG ACCTTCTGCC TTGCAAAGAG GGGAAAGTTG TATGAGGGCA 
GAAAAGCCCA CCACTGGCCA CCATCGGCCA CTCCGGAGCA TTGCCAGGTT CTCCTCAAAA 
TCTAAATCTG TAGACAAATC AGATGAAGAA CTACAGTTTC CCAAAGAGCT GATGGAGGAC 
TGGAGTACTA TGGAGGTGTG TGTAGACTGC AAAAAGTTTA TTTCGGAAAT CATCAGTTCA 
AGCCGGCGCA GCCTGGTGTT GGCCAACAAA AGAGCCCGAT TAAAAAGGAA AACACAGTCT 
TTCTATATGT CCTCACCAGG CCCCTCGGAA TACTGCCCTT CAGAGAGGAC TATCAATGAA 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SPIRE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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