ZNF521 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF521
Entrez Gene ID	490511
Full Name	zinc finger protein 521
General protein information	Preferred Names zinc finger protein 521 Names zinc finger protein 521
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_547633.6	XP_547633.2	zinc finger protein 521 isoform X1
XM_014115493.1	XP_013970968.1	zinc finger protein 521 isoform X1
XM_014115495.1	XP_013970970.1	zinc finger protein 521 isoform X4
XM_547633.5	XP_547633.2	zinc finger protein 521 isoform X1
XM_014115494.1	XP_013970969.1	zinc finger protein 521 isoform X2
XM_022421516.1	XP_022277224.1	zinc finger protein 521 isoform X2
XM_014115494.2	XP_013970969.1	zinc finger protein 521 isoform X3

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ZNF521	NP_001098889.1
Mus musculus (house mouse)	Zfp521	NP_663467.1
Pan troglodytes (chimpanzee)	ZNF521	XP_512068.2
Rattus norvegicus (Norway rat)	Zfp521	NP_001100873.1
Canis lupus familiaris (dog)	ZNF521	XP_547633.2
Gallus gallus (chicken)	ZNF521	XP_004939860.1
Xenopus tropicalis (tropical clawed frog)	znf521	XP_004915266.1
Homo sapiens (human)	ZNF521	NP_056276.1
	LOC709169	XP_002800935.1

Gene Ontology

The following ZNF521 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF521 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe289816	XM_022421516.1 Latest version!	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe37669	XM_014115494.2 Latest version!	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe37670	XM_014115495.1 Latest version!	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe09800	XM_014115493.1 Latest version!	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe09800	XM_547633.6 Latest version!	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OCe09800	XM_547633.5	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe37669	XM_014115494.1	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF521 ( XM_022421516.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277224.1 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe289816
Clone ID Related Accession (Same CDS sequence)	XM_022421516.1
Accession Version	XM_022421516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	zinc finger protein 521 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTTCATGTTG CAAATCACAT GATTGATGAG 
GGACTGAACC ATGAGTGTAA ACTCTGCAGC CAGACCTTCG ACTCCCCTGC CAAACTCCAG 
TGCCATCTGA TAGAGCACAG CTTCGAAGGA ATGGGAGGTA CCTTCAAGTG CCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTATGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCATA CGATGACCCA ACACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277224.1
CDS	141..4073
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_022421516.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421516.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGACCT ATCAGTGCAT CAAGTGTCAG 
ATGGTTTTCT ACAATGAATG GGATATTCAG GTTCATGTTG CAAATCACAT GATTGATGAG 
GGACTGAACC ATGAGTGTAA ACTCTGCAGC CAGACCTTCG ACTCCCCTGC CAAACTCCAG 
TGCCATCTGA TAGAGCACAG CTTCGAAGGA ATGGGAGGTA CCTTCAAGTG CCCCGTCTGC 
TTTACAGTGT TTGTTCAAGC AAACAAGTTG CAGCAGCATA TTTTCTCTGC CCATGGACAA 
GAAGACAAGA TCTATGACTG CACACAATGT CCACAGAAGT TTTTCTTCCA AACAGAGCTG 
CAGAACCATA CGATGACCCA ACACAGCAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_014115494.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970969.1 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe37669
Clone ID Related Accession (Same CDS sequence)	XM_014115494.1 , XM_014115494.2
Accession Version	XM_014115494.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	zinc finger protein 521 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATAT GGAGTGGATG TTGAAGATGA TCCCACTTGC 
TCTTGGCCAG CTTCCTCGCC CTCTAGCAAG GACCAGACTT CCCCTAGCCA TGGAGAAGGT 
TGCGATTTTG GAGAGGAAGA AGGTGGCCCT GGACTCCCAT ACCCATGCCA ATTCTGTGAC 
AAGTCCTTTA GCCGCCTCAG CTACCTAAAG CACCATGAGC AGAGTCATAG CGACAAACTG 
CCTTTCAAAT GCACCTATTG CAGTCGGCTG TTCAAACACA AGCGGAGTCG AGACCGCCAC 
ATCAAACTCC ACACAGGGGA CAAGAAATAC CACTGCAGTG AATGTGATGC CGCATTCTCC 
AGAAGTGATC ACTTAAAGAT CCACTTAAAG ACTCACACGT CCAACAAGCC ATATAAATGT 
GCCATTTGTC GCCGTGGGTT CCTGTCCTCT AGTTCCTTAC ATGGACACAT GCAGGTTCAC 
GAGCGGAACA AGGACGGGTC CCAGTCTGGC TCCAGGATGG AGGACTGGAA GATGAAGGAC 
ACTCAGAAGT GCAGTCAGTG CGAGGAAGGC TTTGATTTTC CGGAAGACCT CCAGAAGCAC 
ATCGCCGAGT GCCACCCCGA GTGTTCCCCC AACGAGGACC GAGCGGCCCT CCAGTGTATC 
TACTGCCATG AGCTCTTTGT GGAGGAGACT TCCCTCGTGA ACCACATGGA GCAGGTGCAT 
GGTGGGGAGA AGAAGAACTC TTGCAGCATT TGCTCAGAGA CTTTCCACAC GGTTGAGGAA 
CTGTACAGCC ACATGGATAG CCACCAGCAA CCGGAGTCAT GCAACCACAG CAACAGCCCT 
TCGCTGGTCA CGGTGGGCTA CACTTCCGTG TCCAGCACGA CTCCGGATTC CAACCTCTCA 
GTGGACAGCT CAACCATGGT GGAAGCTGCC CCGCCCATCC CAAAGAGTCG AGGGAGGAAG 
CGGGCTGCTC AGCAAACCCC TGACATGACG GTTCCCTCGA GTAAACAGGC AAAAGTTACC 
TATAGCTGTA TTTACTGCAA CAAGCAGTTG TTTTCAAGCC TGGCAGTTCT GCAGATTCAC 
CTGAAAACTA TGCATTTAGA TAAGCCAGAA CAGGCCCATA TTTGTCAGTA TTGCTTAGAG 
GTGTTGCCCT CACTCTATAA CCTAAACGAA CATCTTAAGC AAGTGCATGA AGCTCAGGAC 
CCCGGTCTGA TTGTCTCTGC CATGCCTGCC ATAGTCTACC AGTGCAACTT CTGCTCCGAA 
GTTGTCAATG ACCTCAACAC CCTTCAGGAG CACATCCGAT GTTCTCACGG ATTTGCAAAC 
CCTGCCGCTA AGGATAGCAA CGCGTTCTTT TGTCCCCATT GCTACATGGG GTTTCTCACT 
GACTCTTCCC TAGAAGAGCA TATAAGACAG GTCCATTGTG ACCTCAGTGG CTCCCGGTTT 
GGCTCTCCAG TTCTTGGAAC TCCAAAAGAA CCAGTAGTAG AAGTCTATTC TTGTTCTTAC 
TGTACAAATT CGCCAATATT CAACAGCGTT CTTAAGCTGA ACAAGCATAT CAAAGAGAAT 
CACAAGAACA TTCCCTTGGC CCTGAATTAT ATTCACAATG GGAAGAAATC CAGGGCCTTG 
AGCCCTTTAT CTCCTGTGGC CATAGAGCAG ACGTCTCTTA AGATGATGCA GGCGGTGGGA 
GGTGCGCCTG CGCGTCCGGC TGGAGAATAC ATCTGTAATC AGTGTGGTGC TAAGTACACG 
TCCCTAGATA GCTTTCAGAC TCACCTAAAA ACTCATCTCG ACACCGTGCT TCCAAAACTG 
ACCTGCCCAC AGTGCAACAA GGAATTTCCC AACCAGGAAT CCTTGCTGAA GCATGTTACT 
ATCCATTTTA TGATCACCTC AACGTATTAT ATCTGCGAGA GTTGTGATAA GCAGTTCACC 
TCAGTGGACG ACCTTCAGAA GCACCTGCTG GACATGCACA CGTTTGTCTT CTTCCGGTGC 
ACCCTCTGCC AAGAGGTTTT TGACTCAAAA GTCTCCATTC AACTCCACCT GGCCGTAAAG 
CACAGTAACG AAAAGAAGGT CTACCGGTGC ACGTCTTGCA ACTGGGACTT CCGCAATGAG 
ACTGACTTAC AGCTCCACGT GAAACACAAC CACCTGGAAA ACCAGGGGAA GGTGCACAAG 
TGCATTTTCT GCGGGGAGTC TTTCGGCACT GAGGTGGAGC TGCAGTGCCA CATCACCACT 
CACAGTAAGA AGTACAATTG CAAATTCTGC AGCAAAGCCT TCCATGCCAT CATCTTGCTG 
GAGAAACACT TGCGGGAAAA GCATTGCGTC TTTGAGACCA AGACTCCCAA CTGTGGGACG 
AATGGGGCTT CTGAGCAAGT GCAGAAGGAG GAGGTGGAGC TGCAGACCTT GCTGACCAAC 
AGCCAGGAGT CACACAACAG TCACGACGGC AGCGAAGAAG ATGTGGACAC CTCGGAGCCT 
ATGTATGGGT GCGACATCTG TGGGGCCGCC TACACCATGG AGACTCTGCT GCAGAACCAC 
CAGCTCCGAG ACCACAACAT CAGGCCCGGA GAGAGTGCCA TCGTGAAGAA GAAAGCCGAG 
CTCATTAAGG GGAATTATAA GTGTAACGTG TGCTCTCGAA CCTTCTTCTC TGAAAACGGC 
CTCCGTGAAC ACATGCAGAC CCACCTCGGC CCTGTCAAAC ACTACATGTG CCCTATTTGT 
GGAGAGCGGT TTCCCTCCCT TTTGACCCTT ACCGAACACA AAGTCACACA CAGTAAGAGT 
CTCGACACCG GAAACTGCCG GATTTGCAAG ATGCCTCTCC AGAGTGAAGA GGAGTTTTTA 
GAGCATTGCC AAATGCACCC TGACTTGCGG AATTCCCTGA CGGGATTTCG CTGCGTGGTC 
TGCATGCAGA CAGTGACCTC CACCTTAGAA CTCAAAATCC ACGGGACATT TCACATGCAA 
AAGACCGGGA ATGGGTCCAC AGTCCAGACC ACGGGGCGTG GCCAGCACGT CCAAAAACTG 
TACAAGTGTG CGTCTTGCCT CAAAGAGTTC CGTTCCAAGC AAGATCTGGT GAAACTTGAC 
ATCAACGGCC TGCCATATGG TCTGTGCGCC GGCTGTGTGA ATCTCAGCAA GAGTGGCAGC 
CCAGGCATCA ACATCCCTCC CGGCACGAAT AGGCCGGGCT TGGGCCAGAA TGAGAATCTC 
AGTGCCATCG AGGGGAAAGG CAAGGCAGGG GGACTGAAGA CTCGCTGTTC TAGCTGCAAC 
GTTAAGTTTG AGTCTGAAAG TGAACTCCAG AACCACATCC AAACGGTCCA CCGAGAGCTT 
GTGCCAGATA GCAACAGCAC ACAGTTGAAA ACTCCACAAG TGTCGCCAAT GCCCAGAATC 
AGTCCCTCCC AGTCGGATGA GAAGAAGACC TATCAGTGCA TCAAGTGTCA GATGGTTTTC 
TACAATGAAT GGGATATTCA GGTTCATGTT GCAAATCACA TGATTGATGA GGGACTGAAC 
CATGAGTGTA AACTCTGCAG CCAGACCTTC GACTCCCCTG CCAAACTCCA GTGCCATCTG 
ATAGAGCACA GCTTCGAAGG AATGGGAGGT ACCTTCAAGT GCCCCGTCTG CTTTACAGTG 
TTTGTTCAAG CAAACAAGTT GCAGCAGCAT ATTTTCTCTG CCCATGGACA AGAAGACAAG 
ATCTATGACT GCACACAATG TCCACAGAAG TTTTTCTTCC AAACAGAGCT GCAGAACCAT 
ACGATGACCC AACACAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970969.1
CDS	89..4012
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQYGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_014115494.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115494.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATAT GGAGTGGATG TTGAAGATGA TCCCACTTGC 
TCTTGGCCAG CTTCCTCGCC CTCTAGCAAG GACCAGACTT CCCCTAGCCA TGGAGAAGGT 
TGCGATTTTG GAGAGGAAGA AGGTGGCCCT GGACTCCCAT ACCCATGCCA ATTCTGTGAC 
AAGTCCTTTA GCCGCCTCAG CTACCTAAAG CACCATGAGC AGAGTCATAG CGACAAACTG 
CCTTTCAAAT GCACCTATTG CAGTCGGCTG TTCAAACACA AGCGGAGTCG AGACCGCCAC 
ATCAAACTCC ACACAGGGGA CAAGAAATAC CACTGCAGTG AATGTGATGC CGCATTCTCC 
AGAAGTGATC ACTTAAAGAT CCACTTAAAG ACTCACACGT CCAACAAGCC ATATAAATGT 
GCCATTTGTC GCCGTGGGTT CCTGTCCTCT AGTTCCTTAC ATGGACACAT GCAGGTTCAC 
GAGCGGAACA AGGACGGGTC CCAGTCTGGC TCCAGGATGG AGGACTGGAA GATGAAGGAC 
ACTCAGAAGT GCAGTCAGTG CGAGGAAGGC TTTGATTTTC CGGAAGACCT CCAGAAGCAC 
ATCGCCGAGT GCCACCCCGA GTGTTCCCCC AACGAGGACC GAGCGGCCCT CCAGTGTATC 
TACTGCCATG AGCTCTTTGT GGAGGAGACT TCCCTCGTGA ACCACATGGA GCAGGTGCAT 
GGTGGGGAGA AGAAGAACTC TTGCAGCATT TGCTCAGAGA CTTTCCACAC GGTTGAGGAA 
CTGTACAGCC ACATGGATAG CCACCAGCAA CCGGAGTCAT GCAACCACAG CAACAGCCCT 
TCGCTGGTCA CGGTGGGCTA CACTTCCGTG TCCAGCACGA CTCCGGATTC CAACCTCTCA 
GTGGACAGCT CAACCATGGT GGAAGCTGCC CCGCCCATCC CAAAGAGTCG AGGGAGGAAG 
CGGGCTGCTC AGCAAACCCC TGACATGACG GTTCCCTCGA GTAAACAGGC AAAAGTTACC 
TATAGCTGTA TTTACTGCAA CAAGCAGTTG TTTTCAAGCC TGGCAGTTCT GCAGATTCAC 
CTGAAAACTA TGCATTTAGA TAAGCCAGAA CAGGCCCATA TTTGTCAGTA TTGCTTAGAG 
GTGTTGCCCT CACTCTATAA CCTAAACGAA CATCTTAAGC AAGTGCATGA AGCTCAGGAC 
CCCGGTCTGA TTGTCTCTGC CATGCCTGCC ATAGTCTACC AGTGCAACTT CTGCTCCGAA 
GTTGTCAATG ACCTCAACAC CCTTCAGGAG CACATCCGAT GTTCTCACGG ATTTGCAAAC 
CCTGCCGCTA AGGATAGCAA CGCGTTCTTT TGTCCCCATT GCTACATGGG GTTTCTCACT 
GACTCTTCCC TAGAAGAGCA TATAAGACAG GTCCATTGTG ACCTCAGTGG CTCCCGGTTT 
GGCTCTCCAG TTCTTGGAAC TCCAAAAGAA CCAGTAGTAG AAGTCTATTC TTGTTCTTAC 
TGTACAAATT CGCCAATATT CAACAGCGTT CTTAAGCTGA ACAAGCATAT CAAAGAGAAT 
CACAAGAACA TTCCCTTGGC CCTGAATTAT ATTCACAATG GGAAGAAATC CAGGGCCTTG 
AGCCCTTTAT CTCCTGTGGC CATAGAGCAG ACGTCTCTTA AGATGATGCA GGCGGTGGGA 
GGTGCGCCTG CGCGTCCGGC TGGAGAATAC ATCTGTAATC AGTGTGGTGC TAAGTACACG 
TCCCTAGATA GCTTTCAGAC TCACCTAAAA ACTCATCTCG ACACCGTGCT TCCAAAACTG 
ACCTGCCCAC AGTGCAACAA GGAATTTCCC AACCAGGAAT CCTTGCTGAA GCATGTTACT 
ATCCATTTTA TGATCACCTC AACGTATTAT ATCTGCGAGA GTTGTGATAA GCAGTTCACC 
TCAGTGGACG ACCTTCAGAA GCACCTGCTG GACATGCACA CGTTTGTCTT CTTCCGGTGC 
ACCCTCTGCC AAGAGGTTTT TGACTCAAAA GTCTCCATTC AACTCCACCT GGCCGTAAAG 
CACAGTAACG AAAAGAAGGT CTACCGGTGC ACGTCTTGCA ACTGGGACTT CCGCAATGAG 
ACTGACTTAC AGCTCCACGT GAAACACAAC CACCTGGAAA ACCAGGGGAA GGTGCACAAG 
TGCATTTTCT GCGGGGAGTC TTTCGGCACT GAGGTGGAGC TGCAGTGCCA CATCACCACT 
CACAGTAAGA AGTACAATTG CAAATTCTGC AGCAAAGCCT TCCATGCCAT CATCTTGCTG 
GAGAAACACT TGCGGGAAAA GCATTGCGTC TTTGAGACCA AGACTCCCAA CTGTGGGACG 
AATGGGGCTT CTGAGCAAGT GCAGAAGGAG GAGGTGGAGC TGCAGACCTT GCTGACCAAC 
AGCCAGGAGT CACACAACAG TCACGACGGC AGCGAAGAAG ATGTGGACAC CTCGGAGCCT 
ATGTATGGGT GCGACATCTG TGGGGCCGCC TACACCATGG AGACTCTGCT GCAGAACCAC 
CAGCTCCGAG ACCACAACAT CAGGCCCGGA GAGAGTGCCA TCGTGAAGAA GAAAGCCGAG 
CTCATTAAGG GGAATTATAA GTGTAACGTG TGCTCTCGAA CCTTCTTCTC TGAAAACGGC 
CTCCGTGAAC ACATGCAGAC CCACCTCGGC CCTGTCAAAC ACTACATGTG CCCTATTTGT 
GGAGAGCGGT TTCCCTCCCT TTTGACCCTT ACCGAACACA AAGTCACACA CAGTAAGAGT 
CTCGACACCG GAAACTGCCG GATTTGCAAG ATGCCTCTCC AGAGTGAAGA GGAGTTTTTA 
GAGCATTGCC AAATGCACCC TGACTTGCGG AATTCCCTGA CGGGATTTCG CTGCGTGGTC 
TGCATGCAGA CAGTGACCTC CACCTTAGAA CTCAAAATCC ACGGGACATT TCACATGCAA 
AAGACCGGGA ATGGGTCCAC AGTCCAGACC ACGGGGCGTG GCCAGCACGT CCAAAAACTG 
TACAAGTGTG CGTCTTGCCT CAAAGAGTTC CGTTCCAAGC AAGATCTGGT GAAACTTGAC 
ATCAACGGCC TGCCATATGG TCTGTGCGCC GGCTGTGTGA ATCTCAGCAA GAGTGGCAGC 
CCAGGCATCA ACATCCCTCC CGGCACGAAT AGGCCGGGCT TGGGCCAGAA TGAGAATCTC 
AGTGCCATCG AGGGGAAAGG CAAGGCAGGG GGACTGAAGA CTCGCTGTTC TAGCTGCAAC 
GTTAAGTTTG AGTCTGAAAG TGAACTCCAG AACCACATCC AAACGGTCCA CCGAGAGCTT 
GTGCCAGATA GCAACAGCAC ACAGTTGAAA ACTCCACAAG TGTCGCCAAT GCCCAGAATC 
AGTCCCTCCC AGTCGGATGA GAAGAAGACC TATCAGTGCA TCAAGTGTCA GATGGTTTTC 
TACAATGAAT GGGATATTCA GGTTCATGTT GCAAATCACA TGATTGATGA GGGACTGAAC 
CATGAGTGTA AACTCTGCAG CCAGACCTTC GACTCCCCTG CCAAACTCCA GTGCCATCTG 
ATAGAGCACA GCTTCGAAGG AATGGGAGGT ACCTTCAAGT GCCCCGTCTG CTTTACAGTG 
TTTGTTCAAG CAAACAAGTT GCAGCAGCAT ATTTTCTCTG CCCATGGACA AGAAGACAAG 
ATCTATGACT GCACACAATG TCCACAGAAG TTTTTCTTCC AAACAGAGCT GCAGAACCAT 
ACGATGACCC AACACAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_014115494.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970969.1 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

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CloneID	OCe37669
Clone ID Related Accession (Same CDS sequence)	XM_014115494.1 , XM_014115494.2
Accession Version	XM_014115494.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	zinc finger protein 521 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115494.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATAT GGAGTGGATG TTGAAGATGA TCCCACTTGC 
TCTTGGCCAG CTTCCTCGCC CTCTAGCAAG GACCAGACTT CCCCTAGCCA TGGAGAAGGT 
TGCGATTTTG GAGAGGAAGA AGGTGGCCCT GGACTCCCAT ACCCATGCCA ATTCTGTGAC 
AAGTCCTTTA GCCGCCTCAG CTACCTAAAG CACCATGAGC AGAGTCATAG CGACAAACTG 
CCTTTCAAAT GCACCTATTG CAGTCGGCTG TTCAAACACA AGCGGAGTCG AGACCGCCAC 
ATCAAACTCC ACACAGGGGA CAAGAAATAC CACTGCAGTG AATGTGATGC CGCATTCTCC 
AGAAGTGATC ACTTAAAGAT CCACTTAAAG ACTCACACGT CCAACAAGCC ATATAAATGT 
GCCATTTGTC GCCGTGGGTT CCTGTCCTCT AGTTCCTTAC ATGGACACAT GCAGGTTCAC 
GAGCGGAACA AGGACGGGTC CCAGTCTGGC TCCAGGATGG AGGACTGGAA GATGAAGGAC 
ACTCAGAAGT GCAGTCAGTG CGAGGAAGGC TTTGATTTTC CGGAAGACCT CCAGAAGCAC 
ATCGCCGAGT GCCACCCCGA GTGTTCCCCC AACGAGGACC GAGCGGCCCT CCAGTGTATC 
TACTGCCATG AGCTCTTTGT GGAGGAGACT TCCCTCGTGA ACCACATGGA GCAGGTGCAT 
GGTGGGGAGA AGAAGAACTC TTGCAGCATT TGCTCAGAGA CTTTCCACAC GGTTGAGGAA 
CTGTACAGCC ACATGGATAG CCACCAGCAA CCGGAGTCAT GCAACCACAG CAACAGCCCT 
TCGCTGGTCA CGGTGGGCTA CACTTCCGTG TCCAGCACGA CTCCGGATTC CAACCTCTCA 
GTGGACAGCT CAACCATGGT GGAAGCTGCC CCGCCCATCC CAAAGAGTCG AGGGAGGAAG 
CGGGCTGCTC AGCAAACCCC TGACATGACG GTTCCCTCGA GTAAACAGGC AAAAGTTACC 
TATAGCTGTA TTTACTGCAA CAAGCAGTTG TTTTCAAGCC TGGCAGTTCT GCAGATTCAC 
CTGAAAACTA TGCATTTAGA TAAGCCAGAA CAGGCCCATA TTTGTCAGTA TTGCTTAGAG 
GTGTTGCCCT CACTCTATAA CCTAAACGAA CATCTTAAGC AAGTGCATGA AGCTCAGGAC 
CCCGGTCTGA TTGTCTCTGC CATGCCTGCC ATAGTCTACC AGTGCAACTT CTGCTCCGAA 
GTTGTCAATG ACCTCAACAC CCTTCAGGAG CACATCCGAT GTTCTCACGG ATTTGCAAAC 
CCTGCCGCTA AGGATAGCAA CGCGTTCTTT TGTCCCCATT GCTACATGGG GTTTCTCACT 
GACTCTTCCC TAGAAGAGCA TATAAGACAG GTCCATTGTG ACCTCAGTGG CTCCCGGTTT 
GGCTCTCCAG TTCTTGGAAC TCCAAAAGAA CCAGTAGTAG AAGTCTATTC TTGTTCTTAC 
TGTACAAATT CGCCAATATT CAACAGCGTT CTTAAGCTGA ACAAGCATAT CAAAGAGAAT 
CACAAGAACA TTCCCTTGGC CCTGAATTAT ATTCACAATG GGAAGAAATC CAGGGCCTTG 
AGCCCTTTAT CTCCTGTGGC CATAGAGCAG ACGTCTCTTA AGATGATGCA GGCGGTGGGA 
GGTGCGCCTG CGCGTCCGGC TGGAGAATAC ATCTGTAATC AGTGTGGTGC TAAGTACACG 
TCCCTAGATA GCTTTCAGAC TCACCTAAAA ACTCATCTCG ACACCGTGCT TCCAAAACTG 
ACCTGCCCAC AGTGCAACAA GGAATTTCCC AACCAGGAAT CCTTGCTGAA GCATGTTACT 
ATCCATTTTA TGATCACCTC AACGTATTAT ATCTGCGAGA GTTGTGATAA GCAGTTCACC 
TCAGTGGACG ACCTTCAGAA GCACCTGCTG GACATGCACA CGTTTGTCTT CTTCCGGTGC 
ACCCTCTGCC AAGAGGTTTT TGACTCAAAA GTCTCCATTC AACTCCACCT GGCCGTAAAG 
CACAGTAACG AAAAGAAGGT CTACCGGTGC ACGTCTTGCA ACTGGGACTT CCGCAATGAG 
ACTGACTTAC AGCTCCACGT GAAACACAAC CACCTGGAAA ACCAGGGGAA GGTGCACAAG 
TGCATTTTCT GCGGGGAGTC TTTCGGCACT GAGGTGGAGC TGCAGTGCCA CATCACCACT 
CACAGTAAGA AGTACAATTG CAAATTCTGC AGCAAAGCCT TCCATGCCAT CATCTTGCTG 
GAGAAACACT TGCGGGAAAA GCATTGCGTC TTTGAGACCA AGACTCCCAA CTGTGGGACG 
AATGGGGCTT CTGAGCAAGT GCAGAAGGAG GAGGTGGAGC TGCAGACCTT GCTGACCAAC 
AGCCAGGAGT CACACAACAG TCACGACGGC AGCGAAGAAG ATGTGGACAC CTCGGAGCCT 
ATGTATGGGT GCGACATCTG TGGGGCCGCC TACACCATGG AGACTCTGCT GCAGAACCAC 
CAGCTCCGAG ACCACAACAT CAGGCCCGGA GAGAGTGCCA TCGTGAAGAA GAAAGCCGAG 
CTCATTAAGG GGAATTATAA GTGTAACGTG TGCTCTCGAA CCTTCTTCTC TGAAAACGGC 
CTCCGTGAAC ACATGCAGAC CCACCTCGGC CCTGTCAAAC ACTACATGTG CCCTATTTGT 
GGAGAGCGGT TTCCCTCCCT TTTGACCCTT ACCGAACACA AAGTCACACA CAGTAAGAGT 
CTCGACACCG GAAACTGCCG GATTTGCAAG ATGCCTCTCC AGAGTGAAGA GGAGTTTTTA 
GAGCATTGCC AAATGCACCC TGACTTGCGG AATTCCCTGA CGGGATTTCG CTGCGTGGTC 
TGCATGCAGA CAGTGACCTC CACCTTAGAA CTCAAAATCC ACGGGACATT TCACATGCAA 
AAGACCGGGA ATGGGTCCAC AGTCCAGACC ACGGGGCGTG GCCAGCACGT CCAAAAACTG 
TACAAGTGTG CGTCTTGCCT CAAAGAGTTC CGTTCCAAGC AAGATCTGGT GAAACTTGAC 
ATCAACGGCC TGCCATATGG TCTGTGCGCC GGCTGTGTGA ATCTCAGCAA GAGTGGCAGC 
CCAGGCATCA ACATCCCTCC CGGCACGAAT AGGCCGGGCT TGGGCCAGAA TGAGAATCTC 
AGTGCCATCG AGGGGAAAGG CAAGGCAGGG GGACTGAAGA CTCGCTGTTC TAGCTGCAAC 
GTTAAGTTTG AGTCTGAAAG TGAACTCCAG AACCACATCC AAACGGTCCA CCGAGAGCTT 
GTGCCAGATA GCAACAGCAC ACAGTTGAAA ACTCCACAAG TGTCGCCAAT GCCCAGAATC 
AGTCCCTCCC AGTCGGATGA GAAGAAGACC TATCAGTGCA TCAAGTGTCA GATGGTTTTC 
TACAATGAAT GGGATATTCA GGTTCATGTT GCAAATCACA TGATTGATGA GGGACTGAAC 
CATGAGTGTA AACTCTGCAG CCAGACCTTC GACTCCCCTG CCAAACTCCA GTGCCATCTG 
ATAGAGCACA GCTTCGAAGG AATGGGAGGT ACCTTCAAGT GCCCCGTCTG CTTTACAGTG 
TTTGTTCAAG CAAACAAGTT GCAGCAGCAT ATTTTCTCTG CCCATGGACA AGAAGACAAG 
ATCTATGACT GCACACAATG TCCACAGAAG TTTTTCTTCC AAACAGAGCT GCAGAACCAT 
ACGATGACCC AACACAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970969.1
CDS	141..4064
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQYGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_014115494.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115494.2

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATAT GGAGTGGATG TTGAAGATGA TCCCACTTGC 
TCTTGGCCAG CTTCCTCGCC CTCTAGCAAG GACCAGACTT CCCCTAGCCA TGGAGAAGGT 
TGCGATTTTG GAGAGGAAGA AGGTGGCCCT GGACTCCCAT ACCCATGCCA ATTCTGTGAC 
AAGTCCTTTA GCCGCCTCAG CTACCTAAAG CACCATGAGC AGAGTCATAG CGACAAACTG 
CCTTTCAAAT GCACCTATTG CAGTCGGCTG TTCAAACACA AGCGGAGTCG AGACCGCCAC 
ATCAAACTCC ACACAGGGGA CAAGAAATAC CACTGCAGTG AATGTGATGC CGCATTCTCC 
AGAAGTGATC ACTTAAAGAT CCACTTAAAG ACTCACACGT CCAACAAGCC ATATAAATGT 
GCCATTTGTC GCCGTGGGTT CCTGTCCTCT AGTTCCTTAC ATGGACACAT GCAGGTTCAC 
GAGCGGAACA AGGACGGGTC CCAGTCTGGC TCCAGGATGG AGGACTGGAA GATGAAGGAC 
ACTCAGAAGT GCAGTCAGTG CGAGGAAGGC TTTGATTTTC CGGAAGACCT CCAGAAGCAC 
ATCGCCGAGT GCCACCCCGA GTGTTCCCCC AACGAGGACC GAGCGGCCCT CCAGTGTATC 
TACTGCCATG AGCTCTTTGT GGAGGAGACT TCCCTCGTGA ACCACATGGA GCAGGTGCAT 
GGTGGGGAGA AGAAGAACTC TTGCAGCATT TGCTCAGAGA CTTTCCACAC GGTTGAGGAA 
CTGTACAGCC ACATGGATAG CCACCAGCAA CCGGAGTCAT GCAACCACAG CAACAGCCCT 
TCGCTGGTCA CGGTGGGCTA CACTTCCGTG TCCAGCACGA CTCCGGATTC CAACCTCTCA 
GTGGACAGCT CAACCATGGT GGAAGCTGCC CCGCCCATCC CAAAGAGTCG AGGGAGGAAG 
CGGGCTGCTC AGCAAACCCC TGACATGACG GTTCCCTCGA GTAAACAGGC AAAAGTTACC 
TATAGCTGTA TTTACTGCAA CAAGCAGTTG TTTTCAAGCC TGGCAGTTCT GCAGATTCAC 
CTGAAAACTA TGCATTTAGA TAAGCCAGAA CAGGCCCATA TTTGTCAGTA TTGCTTAGAG 
GTGTTGCCCT CACTCTATAA CCTAAACGAA CATCTTAAGC AAGTGCATGA AGCTCAGGAC 
CCCGGTCTGA TTGTCTCTGC CATGCCTGCC ATAGTCTACC AGTGCAACTT CTGCTCCGAA 
GTTGTCAATG ACCTCAACAC CCTTCAGGAG CACATCCGAT GTTCTCACGG ATTTGCAAAC 
CCTGCCGCTA AGGATAGCAA CGCGTTCTTT TGTCCCCATT GCTACATGGG GTTTCTCACT 
GACTCTTCCC TAGAAGAGCA TATAAGACAG GTCCATTGTG ACCTCAGTGG CTCCCGGTTT 
GGCTCTCCAG TTCTTGGAAC TCCAAAAGAA CCAGTAGTAG AAGTCTATTC TTGTTCTTAC 
TGTACAAATT CGCCAATATT CAACAGCGTT CTTAAGCTGA ACAAGCATAT CAAAGAGAAT 
CACAAGAACA TTCCCTTGGC CCTGAATTAT ATTCACAATG GGAAGAAATC CAGGGCCTTG 
AGCCCTTTAT CTCCTGTGGC CATAGAGCAG ACGTCTCTTA AGATGATGCA GGCGGTGGGA 
GGTGCGCCTG CGCGTCCGGC TGGAGAATAC ATCTGTAATC AGTGTGGTGC TAAGTACACG 
TCCCTAGATA GCTTTCAGAC TCACCTAAAA ACTCATCTCG ACACCGTGCT TCCAAAACTG 
ACCTGCCCAC AGTGCAACAA GGAATTTCCC AACCAGGAAT CCTTGCTGAA GCATGTTACT 
ATCCATTTTA TGATCACCTC AACGTATTAT ATCTGCGAGA GTTGTGATAA GCAGTTCACC 
TCAGTGGACG ACCTTCAGAA GCACCTGCTG GACATGCACA CGTTTGTCTT CTTCCGGTGC 
ACCCTCTGCC AAGAGGTTTT TGACTCAAAA GTCTCCATTC AACTCCACCT GGCCGTAAAG 
CACAGTAACG AAAAGAAGGT CTACCGGTGC ACGTCTTGCA ACTGGGACTT CCGCAATGAG 
ACTGACTTAC AGCTCCACGT GAAACACAAC CACCTGGAAA ACCAGGGGAA GGTGCACAAG 
TGCATTTTCT GCGGGGAGTC TTTCGGCACT GAGGTGGAGC TGCAGTGCCA CATCACCACT 
CACAGTAAGA AGTACAATTG CAAATTCTGC AGCAAAGCCT TCCATGCCAT CATCTTGCTG 
GAGAAACACT TGCGGGAAAA GCATTGCGTC TTTGAGACCA AGACTCCCAA CTGTGGGACG 
AATGGGGCTT CTGAGCAAGT GCAGAAGGAG GAGGTGGAGC TGCAGACCTT GCTGACCAAC 
AGCCAGGAGT CACACAACAG TCACGACGGC AGCGAAGAAG ATGTGGACAC CTCGGAGCCT 
ATGTATGGGT GCGACATCTG TGGGGCCGCC TACACCATGG AGACTCTGCT GCAGAACCAC 
CAGCTCCGAG ACCACAACAT CAGGCCCGGA GAGAGTGCCA TCGTGAAGAA GAAAGCCGAG 
CTCATTAAGG GGAATTATAA GTGTAACGTG TGCTCTCGAA CCTTCTTCTC TGAAAACGGC 
CTCCGTGAAC ACATGCAGAC CCACCTCGGC CCTGTCAAAC ACTACATGTG CCCTATTTGT 
GGAGAGCGGT TTCCCTCCCT TTTGACCCTT ACCGAACACA AAGTCACACA CAGTAAGAGT 
CTCGACACCG GAAACTGCCG GATTTGCAAG ATGCCTCTCC AGAGTGAAGA GGAGTTTTTA 
GAGCATTGCC AAATGCACCC TGACTTGCGG AATTCCCTGA CGGGATTTCG CTGCGTGGTC 
TGCATGCAGA CAGTGACCTC CACCTTAGAA CTCAAAATCC ACGGGACATT TCACATGCAA 
AAGACCGGGA ATGGGTCCAC AGTCCAGACC ACGGGGCGTG GCCAGCACGT CCAAAAACTG 
TACAAGTGTG CGTCTTGCCT CAAAGAGTTC CGTTCCAAGC AAGATCTGGT GAAACTTGAC 
ATCAACGGCC TGCCATATGG TCTGTGCGCC GGCTGTGTGA ATCTCAGCAA GAGTGGCAGC 
CCAGGCATCA ACATCCCTCC CGGCACGAAT AGGCCGGGCT TGGGCCAGAA TGAGAATCTC 
AGTGCCATCG AGGGGAAAGG CAAGGCAGGG GGACTGAAGA CTCGCTGTTC TAGCTGCAAC 
GTTAAGTTTG AGTCTGAAAG TGAACTCCAG AACCACATCC AAACGGTCCA CCGAGAGCTT 
GTGCCAGATA GCAACAGCAC ACAGTTGAAA ACTCCACAAG TGTCGCCAAT GCCCAGAATC 
AGTCCCTCCC AGTCGGATGA GAAGAAGACC TATCAGTGCA TCAAGTGTCA GATGGTTTTC 
TACAATGAAT GGGATATTCA GGTTCATGTT GCAAATCACA TGATTGATGA GGGACTGAAC 
CATGAGTGTA AACTCTGCAG CCAGACCTTC GACTCCCCTG CCAAACTCCA GTGCCATCTG 
ATAGAGCACA GCTTCGAAGG AATGGGAGGT ACCTTCAAGT GCCCCGTCTG CTTTACAGTG 
TTTGTTCAAG CAAACAAGTT GCAGCAGCAT ATTTTCTCTG CCCATGGACA AGAAGACAAG 
ATCTATGACT GCACACAATG TCCACAGAAG TTTTTCTTCC AAACAGAGCT GCAGAACCAT 
ACGATGACCC AACACAGCAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_014115495.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970970.1 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe37670
Clone ID Related Accession (Same CDS sequence)	XM_014115495.1
Accession Version	XM_014115495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	zinc finger protein 521 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970970.1
CDS	789..4064
Translation	MQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_014115495.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115495.1

ORF Insert Sequence:

ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_547633.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547633.2 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe09800
Clone ID Related Accession (Same CDS sequence)	XM_547633.6 , XM_014115493.1 , XM_547633.5
Accession Version	XM_547633.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	zinc finger protein 521 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_547633.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547633.2
CDS	141..4076
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_547633.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547633.6

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_014115493.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970968.1 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

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CloneID	OCe09800
Clone ID Related Accession (Same CDS sequence)	XM_547633.6 , XM_014115493.1 , XM_547633.5
Accession Version	XM_014115493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	zinc finger protein 521 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970968.1
CDS	278..4213
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_014115493.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115493.1

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF521 ( XM_547633.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547633.2 Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

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CloneID	OCe09800
Clone ID Related Accession (Same CDS sequence)	XM_547633.6 , XM_014115493.1 , XM_547633.5
Accession Version	XM_547633.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	zinc finger protein 521 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547633.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547633.2
CDS	89..4024
Translation	MSRRKQAKPRSLKDPNCKLEDKTEDGEAIDCKKRPEDGEELEDEAVHSCDSCLQVFESLSDITEHKINQCQLTDGVDVEDDPTCSWPASSPSSKDQTSPSHGEGCDFGEEEGGPGLPYPCQFCDKSFSRLSYLKHHEQSHSDKLPFKCTYCSRLFKHKRSRDRHIKLHTGDKKYHCSECDAAFSRSDHLKIHLKTHTSNKPYKCAICRRGFLSSSSLHGHMQVHERNKDGSQSGSRMEDWKMKDTQKCSQCEEGFDFPEDLQKHIAECHPECSPNEDRAALQCIYCHELFVEETSLVNHMEQVHGGEKKNSCSICSETFHTVEELYSHMDSHQQPESCNHSNSPSLVTVGYTSVSSTTPDSNLSVDSSTMVEAAPPIPKSRGRKRAAQQTPDMTVPSSKQAKVTYSCIYCNKQLFSSLAVLQIHLKTMHLDKPEQAHICQYCLEVLPSLYNLNEHLKQVHEAQDPGLIVSAMPAIVYQCNFCSEVVNDLNTLQEHIRCSHGFANPAAKDSNAFFCPHCYMGFLTDSSLEEHIRQVHCDLSGSRFGSPVLGTPKEPVVEVYSCSYCTNSPIFNSVLKLNKHIKENHKNIPLALNYIHNGKKSRALSPLSPVAIEQTSLKMMQAVGGAPARPAGEYICNQCGAKYTSLDSFQTHLKTHLDTVLPKLTCPQCNKEFPNQESLLKHVTIHFMITSTYYICESCDKQFTSVDDLQKHLLDMHTFVFFRCTLCQEVFDSKVSIQLHLAVKHSNEKKVYRCTSCNWDFRNETDLQLHVKHNHLENQGKVHKCIFCGESFGTEVELQCHITTHSKKYNCKFCSKAFHAIILLEKHLREKHCVFETKTPNCGTNGASEQVQKEEVELQTLLTNSQESHNSHDGSEEDVDTSEPMYGCDICGAAYTMETLLQNHQLRDHNIRPGESAIVKKKAELIKGNYKCNVCSRTFFSENGLREHMQTHLGPVKHYMCPICGERFPSLLTLTEHKVTHSKSLDTGNCRICKMPLQSEEEFLEHCQMHPDLRNSLTGFRCVVCMQTVTSTLELKIHGTFHMQKTGNGSTVQTTGRGQHVQKLYKCASCLKEFRSKQDLVKLDINGLPYGLCAGCVNLSKSGSPGINIPPGTNRPGLGQNENLSAIEGKGKAGGLKTRCSSCNVKFESESELQNHIQTVHRELVPDSNSTQLKTPQVSPMPRISPSQSDEKKTYQCIKCQMVFYNEWDIQVHVANHMIDEGLNHECKLCSQTFDSPAKLQCHLIEHSFEGMGGTFKCPVCFTVFVQANKLQQHIFSAHGQEDKIYDCTQCPQKFFFQTELQNHTMTQHSS

Target ORF information:

RefSeq Version	XM_547633.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris zinc finger protein 521 (ZNF521), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547633.5

ORF Insert Sequence:

ATGTCTCGCC GCAAGCAAGC GAAACCGAGA TCCCTCAAAG ACCCCAACTG TAAACTTGAA 
GACAAGACTG AAGATGGAGA GGCAATAGAC TGTAAGAAGA GGCCGGAAGA CGGCGAGGAG 
CTGGAAGACG AAGCTGTGCA CAGCTGTGAC AGCTGCCTCC AGGTGTTCGA GTCACTGAGC 
GATATCACCG AACACAAGAT TAATCAATGT CAGTTGACAG ATGGAGTGGA TGTTGAAGAT 
GATCCCACTT GCTCTTGGCC AGCTTCCTCG CCCTCTAGCA AGGACCAGAC TTCCCCTAGC 
CATGGAGAAG GTTGCGATTT TGGAGAGGAA GAAGGTGGCC CTGGACTCCC ATACCCATGC 
CAATTCTGTG ACAAGTCCTT TAGCCGCCTC AGCTACCTAA AGCACCATGA GCAGAGTCAT 
AGCGACAAAC TGCCTTTCAA ATGCACCTAT TGCAGTCGGC TGTTCAAACA CAAGCGGAGT 
CGAGACCGCC ACATCAAACT CCACACAGGG GACAAGAAAT ACCACTGCAG TGAATGTGAT 
GCCGCATTCT CCAGAAGTGA TCACTTAAAG ATCCACTTAA AGACTCACAC GTCCAACAAG 
CCATATAAAT GTGCCATTTG TCGCCGTGGG TTCCTGTCCT CTAGTTCCTT ACATGGACAC 
ATGCAGGTTC ACGAGCGGAA CAAGGACGGG TCCCAGTCTG GCTCCAGGAT GGAGGACTGG 
AAGATGAAGG ACACTCAGAA GTGCAGTCAG TGCGAGGAAG GCTTTGATTT TCCGGAAGAC 
CTCCAGAAGC ACATCGCCGA GTGCCACCCC GAGTGTTCCC CCAACGAGGA CCGAGCGGCC 
CTCCAGTGTA TCTACTGCCA TGAGCTCTTT GTGGAGGAGA CTTCCCTCGT GAACCACATG 
GAGCAGGTGC ATGGTGGGGA GAAGAAGAAC TCTTGCAGCA TTTGCTCAGA GACTTTCCAC 
ACGGTTGAGG AACTGTACAG CCACATGGAT AGCCACCAGC AACCGGAGTC ATGCAACCAC 
AGCAACAGCC CTTCGCTGGT CACGGTGGGC TACACTTCCG TGTCCAGCAC GACTCCGGAT 
TCCAACCTCT CAGTGGACAG CTCAACCATG GTGGAAGCTG CCCCGCCCAT CCCAAAGAGT 
CGAGGGAGGA AGCGGGCTGC TCAGCAAACC CCTGACATGA CGGTTCCCTC GAGTAAACAG 
GCAAAAGTTA CCTATAGCTG TATTTACTGC AACAAGCAGT TGTTTTCAAG CCTGGCAGTT 
CTGCAGATTC ACCTGAAAAC TATGCATTTA GATAAGCCAG AACAGGCCCA TATTTGTCAG 
TATTGCTTAG AGGTGTTGCC CTCACTCTAT AACCTAAACG AACATCTTAA GCAAGTGCAT 
GAAGCTCAGG ACCCCGGTCT GATTGTCTCT GCCATGCCTG CCATAGTCTA CCAGTGCAAC 
TTCTGCTCCG AAGTTGTCAA TGACCTCAAC ACCCTTCAGG AGCACATCCG ATGTTCTCAC 
GGATTTGCAA ACCCTGCCGC TAAGGATAGC AACGCGTTCT TTTGTCCCCA TTGCTACATG 
GGGTTTCTCA CTGACTCTTC CCTAGAAGAG CATATAAGAC AGGTCCATTG TGACCTCAGT 
GGCTCCCGGT TTGGCTCTCC AGTTCTTGGA ACTCCAAAAG AACCAGTAGT AGAAGTCTAT 
TCTTGTTCTT ACTGTACAAA TTCGCCAATA TTCAACAGCG TTCTTAAGCT GAACAAGCAT 
ATCAAAGAGA ATCACAAGAA CATTCCCTTG GCCCTGAATT ATATTCACAA TGGGAAGAAA 
TCCAGGGCCT TGAGCCCTTT ATCTCCTGTG GCCATAGAGC AGACGTCTCT TAAGATGATG 
CAGGCGGTGG GAGGTGCGCC TGCGCGTCCG GCTGGAGAAT ACATCTGTAA TCAGTGTGGT 
GCTAAGTACA CGTCCCTAGA TAGCTTTCAG ACTCACCTAA AAACTCATCT CGACACCGTG 
CTTCCAAAAC TGACCTGCCC ACAGTGCAAC AAGGAATTTC CCAACCAGGA ATCCTTGCTG 
AAGCATGTTA CTATCCATTT TATGATCACC TCAACGTATT ATATCTGCGA GAGTTGTGAT 
AAGCAGTTCA CCTCAGTGGA CGACCTTCAG AAGCACCTGC TGGACATGCA CACGTTTGTC 
TTCTTCCGGT GCACCCTCTG CCAAGAGGTT TTTGACTCAA AAGTCTCCAT TCAACTCCAC 
CTGGCCGTAA AGCACAGTAA CGAAAAGAAG GTCTACCGGT GCACGTCTTG CAACTGGGAC 
TTCCGCAATG AGACTGACTT ACAGCTCCAC GTGAAACACA ACCACCTGGA AAACCAGGGG 
AAGGTGCACA AGTGCATTTT CTGCGGGGAG TCTTTCGGCA CTGAGGTGGA GCTGCAGTGC 
CACATCACCA CTCACAGTAA GAAGTACAAT TGCAAATTCT GCAGCAAAGC CTTCCATGCC 
ATCATCTTGC TGGAGAAACA CTTGCGGGAA AAGCATTGCG TCTTTGAGAC CAAGACTCCC 
AACTGTGGGA CGAATGGGGC TTCTGAGCAA GTGCAGAAGG AGGAGGTGGA GCTGCAGACC 
TTGCTGACCA ACAGCCAGGA GTCACACAAC AGTCACGACG GCAGCGAAGA AGATGTGGAC 
ACCTCGGAGC CTATGTATGG GTGCGACATC TGTGGGGCCG CCTACACCAT GGAGACTCTG 
CTGCAGAACC ACCAGCTCCG AGACCACAAC ATCAGGCCCG GAGAGAGTGC CATCGTGAAG 
AAGAAAGCCG AGCTCATTAA GGGGAATTAT AAGTGTAACG TGTGCTCTCG AACCTTCTTC 
TCTGAAAACG GCCTCCGTGA ACACATGCAG ACCCACCTCG GCCCTGTCAA ACACTACATG 
TGCCCTATTT GTGGAGAGCG GTTTCCCTCC CTTTTGACCC TTACCGAACA CAAAGTCACA 
CACAGTAAGA GTCTCGACAC CGGAAACTGC CGGATTTGCA AGATGCCTCT CCAGAGTGAA 
GAGGAGTTTT TAGAGCATTG CCAAATGCAC CCTGACTTGC GGAATTCCCT GACGGGATTT 
CGCTGCGTGG TCTGCATGCA GACAGTGACC TCCACCTTAG AACTCAAAAT CCACGGGACA 
TTTCACATGC AAAAGACCGG GAATGGGTCC ACAGTCCAGA CCACGGGGCG TGGCCAGCAC 
GTCCAAAAAC TGTACAAGTG TGCGTCTTGC CTCAAAGAGT TCCGTTCCAA GCAAGATCTG 
GTGAAACTTG ACATCAACGG CCTGCCATAT GGTCTGTGCG CCGGCTGTGT GAATCTCAGC 
AAGAGTGGCA GCCCAGGCAT CAACATCCCT CCCGGCACGA ATAGGCCGGG CTTGGGCCAG 
AATGAGAATC TCAGTGCCAT CGAGGGGAAA GGCAAGGCAG GGGGACTGAA GACTCGCTGT 
TCTAGCTGCA ACGTTAAGTT TGAGTCTGAA AGTGAACTCC AGAACCACAT CCAAACGGTC 
CACCGAGAGC TTGTGCCAGA TAGCAACAGC ACACAGTTGA AAACTCCACA AGTGTCGCCA 
ATGCCCAGAA TCAGTCCCTC CCAGTCGGAT GAGAAGAAGA CCTATCAGTG CATCAAGTGT 
CAGATGGTTT TCTACAATGA ATGGGATATT CAGGTTCATG TTGCAAATCA CATGATTGAT 
GAGGGACTGA ACCATGAGTG TAAACTCTGC AGCCAGACCT TCGACTCCCC TGCCAAACTC 
CAGTGCCATC TGATAGAGCA CAGCTTCGAA GGAATGGGAG GTACCTTCAA GTGCCCCGTC 
TGCTTTACAG TGTTTGTTCA AGCAAACAAG TTGCAGCAGC ATATTTTCTC TGCCCATGGA 
CAAGAAGACA AGATCTATGA CTGCACACAA TGTCCACAGA AGTTTTTCTT CCAAACAGAG 
CTGCAGAACC ATACGATGAC CCAACACAGC AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ZNF521 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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