LRRC8D cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRRC8D
Entrez Gene ID	490167
Full Name	leucine rich repeat containing 8 VRAC subunit D
General protein information	Preferred Names leucine rich repeat containing 8 VRAC subunit D Names volume-regulated anion channel subunit LRRC8D leucine rich repeat containing 8 family member D
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022420523.1	XP_022276231.1	volume-regulated anion channel subunit LRRC8D
XM_022420526.1	XP_022276234.1	volume-regulated anion channel subunit LRRC8D
XM_014114851.1	XP_013970326.1	volume-regulated anion channel subunit LRRC8D
XM_547288.5	XP_547288.2	volume-regulated anion channel subunit LRRC8D
XM_022420525.1	XP_022276233.1	volume-regulated anion channel subunit LRRC8D
XM_005621949.2	XP_005622006.1	volume-regulated anion channel subunit LRRC8D
XM_022420524.1	XP_022276232.1	volume-regulated anion channel subunit LRRC8D

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRRC8D	NP_060573.2
Gallus gallus (chicken)	LRRC8D	XP_004936686.1
Xenopus tropicalis (tropical clawed frog)	lrrc8d	NP_001120353.1
Pan troglodytes (chimpanzee)	LRRC8D	XP_001150929.1
	LRRC8D	XP_002801674.1
Canis lupus familiaris (dog)	LRRC8D	XP_005622006.1
Bos taurus (cattle)	LRRC8D	NP_001077210.1
Mus musculus (house mouse)	Lrrc8d	NP_848816.3
Danio rerio (zebrafish)	lrrc8db	NP_001070848.1
Rattus norvegicus (Norway rat)	Lrrc8d	NP_001008339.2

Gene Ontology

The following LRRC8D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRRC8D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe08618	XM_547288.5 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08618	XM_005621949.2 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08618	XM_022420524.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08618	XM_022420525.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08618	XM_022420523.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08618	XM_022420526.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08618	XM_014114851.1 Latest version!	Canis lupus familiaris leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRRC8D ( XM_022420523.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276231.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_022420523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276231.1
CDS	659..3235
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_022420523.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420523.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8D ( XM_022420526.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276234.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_022420526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276234.1
CDS	26..2602
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_022420526.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420526.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8D ( XM_014114851.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970326.1 Canis lupus familiaris leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_014114851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970326.1
CDS	253..2829
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_014114851.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014114851.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8D ( XM_547288.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547288.2 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_547288.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547288.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547288.2
CDS	478..3054
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_547288.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547288.5

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8D ( XM_022420525.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276233.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_022420525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276233.1
CDS	416..2992
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_022420525.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420525.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8D ( XM_005621949.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005622006.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_005621949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621949.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005622006.1
CDS	574..3150
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_005621949.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621949.2

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRRC8D ( XM_022420524.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276232.1 Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X3, mRNA.

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CloneID	OCe08618
Clone ID Related Accession (Same CDS sequence)	XM_022420523.1 , XM_022420526.1 , XM_014114851.1 , XM_547288.5 , XM_022420525.1 , XM_005621949.2 , XM_022420524.1
Accession Version	XM_022420524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	volume-regulated anion channel subunit LRRC8D
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276232.1
CDS	583..3159
Translation	MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPPGNADVTTSIPKMEAATDQDRDGRTTSDISFGASAVTPDIPLRATYPHTDSTVPNQETKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTFIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLTGYEVFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLLHMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHVSRNAQDKQELHLFMLSGVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLYVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIATIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISVIPIEIGLLQNLQHLHITGNKVDLLPKQLFKCVKLRTLNLGQNCITFLSEKIGQLSQLTQLELKGNCLDRLPAQLGQCRLLKKSGLVVEDHLFDTLPLEVKEALNQDLNIPFANGI

Target ORF information:

RefSeq Version	XM_022420524.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine rich repeat containing 8 VRAC subunit D (LRRC8D), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420524.1

ORF Insert Sequence:

ATGTTTACCC TTGCGGAAGT TGCTTCACTT AATGACATTC AACCAACTTA CCGAATCCTG 
AAACCATGGT GGGACGTGTT TATGGATTAC CTGGCTGTCG TTATGTTGAT GGTAGCCATC 
TTTGCAGGAA CCATGCAACT TACCAAAGAT CAGGTGGTCT GCTTGCCAGT ATTGCCATCT 
CCTGTAAATT CAAAGGCACA CACACCACCG GGAAATGCCG ACGTTACCAC CAGTATCCCC 
AAGATGGAAG CAGCCACTGA CCAAGACCGA GATGGGCGGA CGACAAGTGA CATTTCCTTC 
GGCGCATCTG CTGTGACACC TGACATACCT CTCAGAGCCA CATATCCTCA CACAGATTCG 
ACAGTTCCAA ATCAGGAGAC AAAGAAAGAG AAGAAAGATC CAACAGGCCG AAAGACAAAC 
TTGGATTTTC AGCAATATGT ATTTATTAAT CAGATGTGTT ACCATCTGGC CCTTCCGTGG 
TATTCTAAGT ACTTTCCATA CCTTGCTCTT ATACATACTA TTATTCTCAT GGTCAGTAGC 
AACTTTTGGT TCAAATATCC CAAAACATGC TCAAAAGTAG AACATTTTGT TTCAATATTA 
GGAAAGTGCT TTGAATCTCC TTGGACTACT AAAGCGTTGT CCGAGACAGC ATGTGAAGAC 
TCGGAGGAAA ACAAGCAGAG AATAACAGGT GCCCAGACTC TACCAAAGCA TGTGTCTACC 
AGCAGCGATG AAGGGAGCCC CAGTGCCAGT ACCCCAATGA TCAACAAAAC GGGCTTCAAA 
TTTTCAGCCG AGAAGCCTGT GATCGAAGTC CCCAGCATGA CCATCCTAGA TAAAAAAGAT 
GGGGAACAGG CCAAAGCCCT GTTTGAGAAA GTAAGGAAGT TCCGTGCTCA TGTGGAAGAC 
AGTGACTTGA TCTACAAACT CTATGTGGTC CAAACATTTA TCAAAACAGC CAAATTCATT 
TTTATCCTCT GCTATACTGC AAACTTTGTC AACGCAATCA GCTTTGAACA CGTCTGCAAG 
CCCAAAGTGG AGCACCTGAC CGGTTATGAG GTATTTGAGT GCACCCACAA CATGGCTTAC 
ATGCTGAAAA AGCTGCTTAT CAGTTACATA TCCATTATTT GTGTTTATGG TTTTATCTGC 
CTCTACACTC TCTTCTGGTT ATTCAGGATA CCTTTGAAGG AATATTCTTT CGAAAAAGTC 
AGAGAAGAGA GCAGTTTCAG TGACATTCCA GATGTCAAGA ACGATTTTGC GTTCCTCCTA 
CACATGGTGG ACCAATATGA TCAGCTGTAT TCGAAGCGCT TCGGTGTGTT CTTGTCAGAA 
GTTAGTGAAA ATAAACTTAG GGAAATTAGT CTGAACCATG AGTGGACATT TGAAAAACTT 
CGGCAGCACG TGTCCCGCAA CGCCCAGGAC AAGCAGGAGC TCCATCTGTT CATGCTGTCG 
GGTGTGCCCG ACGCCGTCTT CGACCTCACA GACCTGGATG TCCTAAAACT TGAACTGATT 
CCAGAAGCTA AAATTCCCGC TAAGATCTCT CAGATGACCA ACCTCCAAGA GCTCCACCTC 
TGCCACTGCC CTGCAAAAGT TGAACAGACT GCTTTTAGCT TCCTCCGAGA TCACTTGAGA 
TGCCTTTATG TGAAGTTCAC CGACGTGGCT GAAATCCCTG CCTGGGTGTA TTTGCTCAAA 
AACCTTCGAG AGTTGTACTT GATAGGCAAC TTGAACTCTG AAAACAATAA GATGATTGGA 
CTCGAGTCTC TCCGAGAGTT GCGGCACCTT AAGATTCTCC ACGTGAAGAG TAACTTGACC 
AAAGTTCCCT CCAACATTAC AGATGTGGCC CCACATCTTA CAAAGTTAGT CATTCATAAT 
GACGGCACTA AACTCTTGGT ACTGAACAGC CTTAAGAAAA TGATGAATGT CGCCGAGCTC 
GAACTTCAGA ACTGTGAGCT GGAGAGAATC CCCCATGCCA TTTTCAGCCT CTCAAATCTG 
CAGGAACTGG ATTTAAAATC AAATAACATA CGCACCATCG AGGAGATCAT CAGTTTCCAG 
CATTTAAAAC GACTGACGTG TTTAAAATTA TGGCATAATA AAATCGCCAC CATTCCTCCC 
TCCATTACCC ATGTCAAAAA CTTGGAGTCG CTTTATTTCT CAAACAATAA GCTCGAATCC 
TTACCGGTGG CAGTGTTCAG TTTACAGAAA CTCAGATGCT TAGATGTCAG CTACAACAAC 
ATTTCAGTGA TTCCGATAGA AATAGGGTTG CTTCAGAACC TACAGCATTT GCATATCACT 
GGGAACAAAG TGGACCTTCT GCCAAAACAG TTGTTCAAAT GCGTGAAGTT GAGGACTTTA 
AATCTGGGGC AAAACTGCAT CACCTTCCTG TCCGAGAAGA TCGGTCAGCT GTCCCAGCTC 
ACTCAGCTGG AGCTGAAGGG GAACTGCTTG GACCGCCTGC CAGCCCAGCT GGGCCAGTGT 
CGCCTGCTCA AGAAAAGCGG GCTTGTTGTG GAAGATCACC TTTTTGACAC CCTGCCACTA 
GAAGTCAAAG AAGCATTGAA TCAAGACCTC AATATCCCCT TCGCGAATGG GATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LRRC8D cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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