RHBDF1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022420327.1	XP_022276035.1	inactive rhomboid protein 1 isoform X1
XM_547222.6	XP_547222.1	inactive rhomboid protein 1 isoform X4
XM_547222.5	XP_547222.1	inactive rhomboid protein 1 isoform X3
XM_005621771.2	XP_005621828.1	inactive rhomboid protein 1 isoform X2
XM_005621770.3	XP_005621827.1	inactive rhomboid protein 1 isoform X1
XM_005621771.3	XP_005621828.1	inactive rhomboid protein 1 isoform X2
XM_005621773.2	XP_005621830.1	inactive rhomboid protein 1 isoform X2
XM_005621773.3	XP_005621830.1	inactive rhomboid protein 1 isoform X2
XM_005621772.1	XP_005621829.1	inactive rhomboid protein 1 isoform X2
XM_005621770.2	XP_005621827.1	inactive rhomboid protein 1 isoform X1
XM_022420328.1	XP_022276036.1	inactive rhomboid protein 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe08413	XM_022420327.1 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08413	XM_005621770.3 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08414	XM_005621773.3 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08414	XM_005621772.1 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08414	XM_005621771.3 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08416	XM_547222.6 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08415	XM_022420328.1 Latest version!	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OCe08416	XM_547222.5	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08414	XM_005621771.2	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08414	XM_005621773.2	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe08413	XM_005621770.2	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

RHBDF1 ( XM_022420327.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276035.1 Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08413
Clone ID Related Accession (Same CDS sequence)	XM_022420327.1 , XM_005621770.3 , XM_005621770.2
Accession Version	XM_022420327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT 
GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG 
TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT 
GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG 
CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC 
AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC 
ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG 
AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC 
CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC 
TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG 
GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC 
GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG 
TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC 
ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC 
CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT 
CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG 
CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC 
ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC 
CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC 
TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG 
CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG 
CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG 
TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG 
CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG 
GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC 
CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG 
TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG 
ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC 
ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC 
TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC 
ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC 
CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC 
TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC 
ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG 
GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT 
TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG 
GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA 
GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG 
TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC 
CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC 
CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276035.1
CDS	334..3039
Translation	MEARPAPRGTMSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_022420327.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420327.1

ORF Insert Sequence:

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT 
GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG 
TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT 
GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG 
CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC 
AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC 
ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG 
AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC 
CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC 
TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG 
GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC 
GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG 
TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC 
ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC 
CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT 
CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG 
CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC 
ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC 
CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC 
TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG 
CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG 
CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG 
TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG 
CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG 
GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC 
CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG 
TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG 
ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC 
ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC 
TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC 
ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC 
CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC 
TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC 
ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG 
GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT 
TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG 
GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA 
GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG 
TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC 
CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC 
CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621770.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621827.1 Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08413
Clone ID Related Accession (Same CDS sequence)	XM_022420327.1 , XM_005621770.3 , XM_005621770.2
Accession Version	XM_005621770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive rhomboid protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT 
GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG 
TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT 
GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG 
CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC 
AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC 
ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG 
AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC 
CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC 
TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG 
GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC 
GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG 
TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC 
ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC 
CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT 
CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG 
CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC 
ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC 
CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC 
TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG 
CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG 
CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG 
TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG 
CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG 
GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC 
CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG 
TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG 
ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC 
ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC 
TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC 
ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC 
CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC 
TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC 
ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG 
GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT 
TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG 
GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA 
GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG 
TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC 
CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC 
CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621827.1
CDS	334..3039
Translation	MEARPAPRGTMSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621770.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621770.3

ORF Insert Sequence:

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT 
GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG 
TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT 
GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG 
CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC 
AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC 
ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG 
AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC 
CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC 
TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG 
GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC 
GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG 
TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC 
ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC 
CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT 
CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG 
CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC 
ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC 
CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC 
TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG 
CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG 
CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG 
TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG 
CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG 
GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC 
CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG 
TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG 
ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC 
ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC 
TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC 
ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC 
CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC 
TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC 
ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG 
GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT 
TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG 
GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA 
GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG 
TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC 
CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC 
CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621770.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621827.1 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe08413
Clone ID Related Accession (Same CDS sequence)	XM_022420327.1 , XM_005621770.3 , XM_005621770.2
Accession Version	XM_005621770.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive rhomboid protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT 
GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG 
TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT 
GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG 
CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC 
AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC 
ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG 
AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC 
CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC 
TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG 
GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC 
GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG 
TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC 
ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC 
CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT 
CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG 
CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC 
ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC 
CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC 
TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG 
CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG 
CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG 
TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG 
CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG 
GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC 
CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG 
TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG 
ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC 
ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC 
TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC 
ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC 
CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC 
TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC 
ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG 
GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT 
TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG 
GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA 
GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG 
TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC 
CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC 
CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621827.1
CDS	100..2805
Translation	MEARPAPRGTMSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621770.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621770.2

ORF Insert Sequence:

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT 
GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG 
TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT 
GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG 
CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC 
AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC 
ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG 
AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC 
CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC 
TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG 
GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC 
GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG 
TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC 
ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC 
CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT 
CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG 
CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC 
ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC 
CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC 
TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG 
CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG 
CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG 
TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG 
CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG 
GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC 
CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG 
TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG 
ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC 
ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC 
TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC 
ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC 
CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC 
TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC 
ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG 
GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT 
TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG 
GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA 
GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG 
TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC 
CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC 
CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621771.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621828.1 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe08414
Clone ID Related Accession (Same CDS sequence)	XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
Accession Version	XM_005621771.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive rhomboid protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621828.1
CDS	371..3046
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621771.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621771.2

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621771.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621828.1 Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

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CloneID	OCe08414
Clone ID Related Accession (Same CDS sequence)	XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
Accession Version	XM_005621771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621828.1
CDS	53..2728
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621771.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621771.3

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621773.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621830.1 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe08414
Clone ID Related Accession (Same CDS sequence)	XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
Accession Version	XM_005621773.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive rhomboid protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621830.1
CDS	102..2777
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621773.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621773.2

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621773.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621830.1 Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe08414
Clone ID Related Accession (Same CDS sequence)	XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
Accession Version	XM_005621773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive rhomboid protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621830.1
CDS	44..2719
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621773.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621773.3

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_005621772.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005621829.1 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe08414
Clone ID Related Accession (Same CDS sequence)	XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
Accession Version	XM_005621772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive rhomboid protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005621829.1
CDS	60..2735
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRSRQVRFGRVHTLPLLGPPAARRALPQRRSLSRSLLRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_005621772.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005621772.1

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT 
GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA 
GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC 
ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC 
CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG 
GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC 
CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT 
GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA 
CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC 
CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC 
TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG 
GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG 
GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC 
CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG 
AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC 
GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT 
GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC 
CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG 
TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC 
TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC 
CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC 
TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG 
GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC 
AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG 
TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT 
GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC 
GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC 
ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG 
GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG 
GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC 
CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT 
GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG 
AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC 
CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT 
GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC 
TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG 
CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC 
GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC 
ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC 
TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC 
TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_547222.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547222.1 Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe08416
Clone ID Related Accession (Same CDS sequence)	XM_547222.6 , XM_547222.5
Accession Version	XM_547222.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive rhomboid protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_547222.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC 
ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC 
CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA 
CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC 
CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG 
GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC 
TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG 
TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG 
TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC 
CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG 
CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG 
GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG 
ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG 
GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT 
CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC 
TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG 
CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA 
GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG 
TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC 
CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC 
TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC 
AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC 
TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT 
GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC 
CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT 
GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG 
GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC 
ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG 
ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC 
TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547222.1
CDS	119..2689
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_547222.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547222.6

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC 
ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC 
CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA 
CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC 
CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG 
GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC 
TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG 
TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG 
TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC 
CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG 
CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG 
GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG 
ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG 
GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT 
CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC 
TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG 
CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA 
GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG 
TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC 
CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC 
TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC 
AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC 
TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT 
GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC 
CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT 
GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG 
GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC 
ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG 
ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC 
TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_547222.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_547222.1 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe08416
Clone ID Related Accession (Same CDS sequence)	XM_547222.6 , XM_547222.5
Accession Version	XM_547222.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive rhomboid protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547222.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC 
ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC 
CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA 
CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC 
CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG 
GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC 
TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG 
TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG 
TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC 
CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG 
CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG 
GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG 
ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG 
GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT 
CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC 
TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG 
CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA 
GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG 
TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC 
CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC 
TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC 
AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC 
TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT 
GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC 
CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT 
GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG 
GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC 
ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG 
ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC 
TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_547222.1
CDS	189..2759
Translation	MSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_547222.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_547222.5

ORF Insert Sequence:

ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC 
CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC 
TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC 
ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG 
TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC 
ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC 
CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC 
CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA 
CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC 
CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG 
GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC 
TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG 
TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG 
TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC 
CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG 
GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG 
CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG 
GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG 
ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG 
GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT 
CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC 
TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC 
TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG 
CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA 
GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG 
TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC 
CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC 
TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC 
AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC 
TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG 
GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT 
GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC 
CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC 
CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT 
GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA 
GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG 
GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC 
ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC 
CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC 
AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG 
ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC 
TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHBDF1 ( XM_022420328.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276036.1 Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.

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CloneID	OCe08415
Clone ID Related Accession (Same CDS sequence)	XM_022420328.1
Accession Version	XM_022420328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2601bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive rhomboid protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGGG GCACAGCTGA CTGGTTTGGA 
GTGAGCAAGG ACAGTGACAG CACCCAGAAG TGGCAGCGCA AGAGCATCCG CCACTGTAGC 
CAGCGCTATG GAAAGCTGAA GCCTCAGGTC ATCCGGGAGC TAGACCTGCC TAGCCAGGAC 
AACGTGTCCC TGACCAGTAC TGAGACCCCC CCTCCACTGT ATGTGGGGCC GTGCCAGCTG 
GGCATGCAGA AGATCATAGA CCCCCTGGCC CGGGGCCGGG CCTTCCGCGT GGCGGATGAC 
ACGGCCGATG GCCTGAGTAC CCCGCACACA CCTGTCACGC CTGGTGCCGC TTCCCTCTGC 
TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC CGGTTCCCGC GGCGACGCAA GCGTGAATCG 
GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT 
GGCACGTTGC GCCGGGTGCA GCGCCGAAGC TTCACTCCCG CCAGCTTCCT GGAGGAGGAC 
ACAGCTGACT TCCCCGACGA GCTTGACACG TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT 
GAAGAGCTGT CCACGTACCC AGACGAGGTG TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG 
GACTGGGAGA AAGCCACGGA GCAGGGCGAC CTCACGGGGG GCGCCCTGGA CCGCAGCGAG 
CTTGAGCGCA GCCACCTGAT GCTGCCCCTG GAACGAGGCT GGAGGAAGCA GAAGGAGGGT 
GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG CTGCGGCAGG AGGTCGTGAG CACGGCGGGT 
CAGCGGCGCG GCCAGCGCAT CGCCATGCCG GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG 
CCGTATGGGC TAGGCATGGT GGGCCGGCTG ACCAACCGCA CGTACCGCAA GCGCATCGAC 
AGCTACGTCA AACGCCAGAT CGAGGACATG GATGACCACA GGCCGTTCTT CACCTACTGG 
CTCACCTTCG TGCACTCGCT CGTCACCATT CTGGCCGTGT GCATCTACGG TATCGCACCT 
GTGGGCTTCT CGCAGCACGA GACCGTGGAC TCGGTGCTGC GGAACCGCGG GGTCTATGAG 
AATGTCAAGT ATGTGCAGCA GGAGAACTTC TGGATCGGGC CCAGCTCGGA GGCTCTCATT 
CACCTGGGTG CCAAGTTCTC GCCCTGCATG CGTCAGGACC CGCAGGTGCA CAGTTTCATC 
CGTGCTGCGC GGGAGCGTGA GAAGCACTCA GCCTGCTGCG TGCGCAATGA CCGGTCGGGC 
TGTGTGCAGA CATCGGAGGA GGAGTGCTCG TCCACGCTGG CTGTGTGGGT GAAGTGGCCC 
TTGCACCCCA GTGCCCCAGA CCTTGCTGGC CACAAGAGGC AGTTTGGCTC TGTCTGCCAC 
CAGGACCCCA GGGTGTGTGA TGAGCCCTCC TCAGAGGACC CCCACGAGTG GCCAGATGAC 
ATCACCAAGT GGCCGATCTG CACCAAAAAC AGCGCCGGGA ACCACACCAA CCACCCGCAC 
ATGGACTGTG TCCTCACAGG CCGGCCCTGC TGCATTGGCA CCAAGGGCAG GTGTGAGATC 
ACCTCCCGGG AGTACTGTGA CTTCATGAGG GGCTACTTCC ACGAGGAGGC CACACTGTGC 
TCCCAGGTGC ATTGCATGGA TGACGTGTGT GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG 
CCCGACCAGT TCTACCGCCT GTGGCTGTCC CTCTTCTTGC ACGCTGGGAT CCTGCACTGC 
CTGGTGTCCG TCTGCTTCCA GATGACCGTC CTGCGGGACC TGGAGAAGTT GGCAGGCTGG 
CACCGCATAG CCATCATCTA CCTGTTGAGT GGTGTCACTG GTAACCTGGC CAGTGCCATT 
TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC 
CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC 
AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC ACCTTCGGGC TGCTGCCCTG GATCGACAAC 
TTCGCTCACA TCTCAGGCTT CATCAGCGGC CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC 
ATCAGCTTCG GCAAGTTTGA CTTGTACCGC AAGCGCTGCC AGATCATCGT CTTCCAGGTG 
GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG ATCCTCTTCT ACTTCTACCC CGTGCGCTGC 
GAGTGGTGCG AGTTCCTCAC CTGCATCCCC TTCACCGACA AGTTCTGCGA GAAGTACGAG 
CTGGATGCCC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276036.1
CDS	334..2934
Translation	MEARPAPRGTMSEARRDSASSLQRKKPPWLKLDIPAAAPPAPEEPSCLQPLRRQAFLRSVSMPAETAPVPSPHPELRRLGLQRQTSITQTIRRGTADWFGVSKDSDSTQKWQRKSIRHCSQRYGKLKPQVIRELDLPSQDNVSLTSTETPPPLYVGPCQLGMQKIIDPLARGRAFRVADDTADGLSTPHTPVTPGAASLCSFSSSRSGFNRFPRRRKRESVAKMSFRAAAALVKGRSVRDGTLRRVQRRSFTPASFLEEDTADFPDELDTSFFAREGVLHEELSTYPDEVFESPSEAALKDWEKATEQGDLTGGALDRSELERSHLMLPLERGWRKQKEGGAAVPQPKVRLRQEVVSTAGQRRGQRIAMPVRKFFAREKRPYGLGMVGRLTNRTYRKRIDSYVKRQIEDMDDHRPFFTYWLTFVHSLVTILAVCIYGIAPVGFSQHETVDSVLRNRGVYENVKYVQQENFWIGPSSEALIHLGAKFSPCMRQDPQVHSFIRAAREREKHSACCVRNDRSGCVQTSEEECSSTLAVWVKWPLHPSAPDLAGHKRQFGSVCHQDPRVCDEPSSEDPHEWPDDITKWPICTKNSAGNHTNHPHMDCVLTGRPCCIGTKGRCEITSREYCDFMRGYFHEEATLCSQVHCMDDVCGLLPFLNPEVPDQFYRLWLSLFLHAGILHCLVSVCFQMTVLRDLEKLAGWHRIAIIYLLSGVTGNLASAIFLPYRAEVGPAGSQFGILACLFVELFQSWQVLARPWRAFFKLSAVVLFLFTFGLLPWIDNFAHISGFISGLFLSFAFLPYISFGKFDLYRKRCQIIVFQVVFLGLLAGLVILFYFYPVRCEWCEFLTCIPFTDKFCEKYELDAQLH

Target ORF information:

RefSeq Version	XM_022420328.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022420328.1

ORF Insert Sequence:

ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG 
GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG 
AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG 
CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGGG GCACAGCTGA CTGGTTTGGA 
GTGAGCAAGG ACAGTGACAG CACCCAGAAG TGGCAGCGCA AGAGCATCCG CCACTGTAGC 
CAGCGCTATG GAAAGCTGAA GCCTCAGGTC ATCCGGGAGC TAGACCTGCC TAGCCAGGAC 
AACGTGTCCC TGACCAGTAC TGAGACCCCC CCTCCACTGT ATGTGGGGCC GTGCCAGCTG 
GGCATGCAGA AGATCATAGA CCCCCTGGCC CGGGGCCGGG CCTTCCGCGT GGCGGATGAC 
ACGGCCGATG GCCTGAGTAC CCCGCACACA CCTGTCACGC CTGGTGCCGC TTCCCTCTGC 
TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC CGGTTCCCGC GGCGACGCAA GCGTGAATCG 
GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT 
GGCACGTTGC GCCGGGTGCA GCGCCGAAGC TTCACTCCCG CCAGCTTCCT GGAGGAGGAC 
ACAGCTGACT TCCCCGACGA GCTTGACACG TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT 
GAAGAGCTGT CCACGTACCC AGACGAGGTG TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG 
GACTGGGAGA AAGCCACGGA GCAGGGCGAC CTCACGGGGG GCGCCCTGGA CCGCAGCGAG 
CTTGAGCGCA GCCACCTGAT GCTGCCCCTG GAACGAGGCT GGAGGAAGCA GAAGGAGGGT 
GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG CTGCGGCAGG AGGTCGTGAG CACGGCGGGT 
CAGCGGCGCG GCCAGCGCAT CGCCATGCCG GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG 
CCGTATGGGC TAGGCATGGT GGGCCGGCTG ACCAACCGCA CGTACCGCAA GCGCATCGAC 
AGCTACGTCA AACGCCAGAT CGAGGACATG GATGACCACA GGCCGTTCTT CACCTACTGG 
CTCACCTTCG TGCACTCGCT CGTCACCATT CTGGCCGTGT GCATCTACGG TATCGCACCT 
GTGGGCTTCT CGCAGCACGA GACCGTGGAC TCGGTGCTGC GGAACCGCGG GGTCTATGAG 
AATGTCAAGT ATGTGCAGCA GGAGAACTTC TGGATCGGGC CCAGCTCGGA GGCTCTCATT 
CACCTGGGTG CCAAGTTCTC GCCCTGCATG CGTCAGGACC CGCAGGTGCA CAGTTTCATC 
CGTGCTGCGC GGGAGCGTGA GAAGCACTCA GCCTGCTGCG TGCGCAATGA CCGGTCGGGC 
TGTGTGCAGA CATCGGAGGA GGAGTGCTCG TCCACGCTGG CTGTGTGGGT GAAGTGGCCC 
TTGCACCCCA GTGCCCCAGA CCTTGCTGGC CACAAGAGGC AGTTTGGCTC TGTCTGCCAC 
CAGGACCCCA GGGTGTGTGA TGAGCCCTCC TCAGAGGACC CCCACGAGTG GCCAGATGAC 
ATCACCAAGT GGCCGATCTG CACCAAAAAC AGCGCCGGGA ACCACACCAA CCACCCGCAC 
ATGGACTGTG TCCTCACAGG CCGGCCCTGC TGCATTGGCA CCAAGGGCAG GTGTGAGATC 
ACCTCCCGGG AGTACTGTGA CTTCATGAGG GGCTACTTCC ACGAGGAGGC CACACTGTGC 
TCCCAGGTGC ATTGCATGGA TGACGTGTGT GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG 
CCCGACCAGT TCTACCGCCT GTGGCTGTCC CTCTTCTTGC ACGCTGGGAT CCTGCACTGC 
CTGGTGTCCG TCTGCTTCCA GATGACCGTC CTGCGGGACC TGGAGAAGTT GGCAGGCTGG 
CACCGCATAG CCATCATCTA CCTGTTGAGT GGTGTCACTG GTAACCTGGC CAGTGCCATT 
TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC 
CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC 
AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC ACCTTCGGGC TGCTGCCCTG GATCGACAAC 
TTCGCTCACA TCTCAGGCTT CATCAGCGGC CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC 
ATCAGCTTCG GCAAGTTTGA CTTGTACCGC AAGCGCTGCC AGATCATCGT CTTCCAGGTG 
GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG ATCCTCTTCT ACTTCTACCC CGTGCGCTGC 
GAGTGGTGCG AGTTCCTCAC CTGCATCCCC TTCACCGACA AGTTCTGCGA GAAGTACGAG 
CTGGATGCCC AGCTCCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RHBDF1
Entrez Gene ID	490101
Full Name	rhomboid 5 homolog 1
General protein information	Preferred Names rhomboid 5 homolog 1 Names inactive rhomboid protein 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Pan troglodytes (chimpanzee)	RHBDF1	XP_510701.3
Rattus norvegicus (Norway rat)	Rhbdf1	NP_001025205.1
Danio rerio (zebrafish)	rhbdf1	NP_001002228.1
Xenopus tropicalis (tropical clawed frog)	rhbdf1	XP_004917998.1
Homo sapiens (human)	RHBDF1	NP_071895.3
Gallus gallus (chicken)	RHBDF1	XP_004945406.1
Canis lupus familiaris (dog)	RHBDF1	XP_005621827.1
Bos taurus (cattle)	RHBDF1	NP_001098870.1
Mus musculus (house mouse)	Rhbdf1	NP_034247.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RHBDF1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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