RHBDF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following RHBDF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHBDF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe08413 XM_022420327.1
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08413 XM_005621770.3
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08414 XM_005621773.3
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08414 XM_005621772.1
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08414 XM_005621771.3
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08416 XM_547222.6
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08415 XM_022420328.1
Latest version!
Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
View Old Accession Versions >>
OCe08416 XM_547222.5 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08414 XM_005621771.2 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08414 XM_005621773.2 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OCe08413 XM_005621770.2 Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe08413
    Clone ID Related Accession (Same CDS sequence) XM_022420327.1 , XM_005621770.3 , XM_005621770.2
    Accession Version XM_022420327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive rhomboid protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT
    GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG
    TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT
    GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG
    CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC
    AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC
    ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG
    AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC
    CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC
    TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG
    GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC
    GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG
    TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC
    ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC
    CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT
    CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG
    CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC
    ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC
    CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC
    TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG
    CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG
    CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG
    TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG
    CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG
    GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC
    CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG
    TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG
    ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC
    ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC
    TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC
    ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC
    CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC
    TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC
    ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG
    GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT
    TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG
    GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA
    GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG
    TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC
    CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC
    CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276035.1
    CDS334..3039
    Translation

    Target ORF information:

    RefSeq Version XM_022420327.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT
    GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG
    TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT
    GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG
    CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC
    AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC
    ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG
    AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC
    CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC
    TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG
    GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC
    GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG
    TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC
    ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC
    CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT
    CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG
    CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC
    ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC
    CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC
    TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG
    CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG
    CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG
    TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG
    CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG
    GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC
    CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG
    TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG
    ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC
    ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC
    TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC
    ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC
    CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC
    TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC
    ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG
    GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT
    TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG
    GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA
    GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG
    TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC
    CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC
    CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08413
    Clone ID Related Accession (Same CDS sequence) XM_022420327.1 , XM_005621770.3 , XM_005621770.2
    Accession Version XM_005621770.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive rhomboid protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT
    GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG
    TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT
    GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG
    CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC
    AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC
    ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG
    AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC
    CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC
    TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG
    GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC
    GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG
    TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC
    ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC
    CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT
    CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG
    CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC
    ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC
    CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC
    TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG
    CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG
    CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG
    TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG
    CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG
    GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC
    CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG
    TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG
    ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC
    ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC
    TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC
    ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC
    CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC
    TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC
    ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG
    GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT
    TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG
    GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA
    GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG
    TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC
    CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC
    CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621827.1
    CDS334..3039
    Translation

    Target ORF information:

    RefSeq Version XM_005621770.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621770.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT
    GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG
    TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT
    GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG
    CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC
    AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC
    ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG
    AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC
    CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC
    TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG
    GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC
    GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG
    TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC
    ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC
    CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT
    CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG
    CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC
    ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC
    CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC
    TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG
    CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG
    CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG
    TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG
    CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG
    GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC
    CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG
    TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG
    ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC
    ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC
    TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC
    ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC
    CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC
    TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC
    ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG
    GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT
    TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG
    GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA
    GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG
    TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC
    CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC
    CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08413
    Clone ID Related Accession (Same CDS sequence) XM_022420327.1 , XM_005621770.3 , XM_005621770.2
    Accession Version XM_005621770.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2706bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive rhomboid protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT
    GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG
    TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT
    GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG
    CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC
    AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC
    ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG
    AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC
    CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC
    TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG
    GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC
    GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG
    TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC
    ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC
    CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT
    CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG
    CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC
    ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC
    CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC
    TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG
    CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG
    CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG
    TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG
    CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG
    GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC
    CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG
    TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG
    ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC
    ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC
    TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC
    ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC
    CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC
    TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC
    ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG
    GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT
    TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG
    GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA
    GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG
    TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC
    CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC
    CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621827.1
    CDS100..2805
    Translation

    Target ORF information:

    RefSeq Version XM_005621770.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621770.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGCC GACAGGTGCG CTTTGGGCGT
    GTCCACACTC TGCCCCTCTT GGGACCCCCT GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG
    TCTCTCTCTC GGTCCCTGCT CAGGGGCACA GCTGACTGGT TTGGAGTGAG CAAGGACAGT
    GACAGCACCC AGAAGTGGCA GCGCAAGAGC ATCCGCCACT GTAGCCAGCG CTATGGAAAG
    CTGAAGCCTC AGGTCATCCG GGAGCTAGAC CTGCCTAGCC AGGACAACGT GTCCCTGACC
    AGTACTGAGA CCCCCCCTCC ACTGTATGTG GGGCCGTGCC AGCTGGGCAT GCAGAAGATC
    ATAGACCCCC TGGCCCGGGG CCGGGCCTTC CGCGTGGCGG ATGACACGGC CGATGGCCTG
    AGTACCCCGC ACACACCTGT CACGCCTGGT GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC
    CGCTCGGGCT TCAACCGGTT CCCGCGGCGA CGCAAGCGTG AATCGGTGGC CAAGATGAGC
    TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC CGCTCTGTTA GGGATGGCAC GTTGCGCCGG
    GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC TTCCTGGAGG AGGACACAGC TGACTTCCCC
    GACGAGCTTG ACACGTCCTT CTTCGCCCGG GAAGGAGTCC TCCATGAAGA GCTGTCCACG
    TACCCAGACG AGGTGTTTGA GTCCCCCTCG GAGGCAGCGC TCAAGGACTG GGAGAAAGCC
    ACGGAGCAGG GCGACCTCAC GGGGGGCGCC CTGGACCGCA GCGAGCTTGA GCGCAGCCAC
    CTGATGCTGC CCCTGGAACG AGGCTGGAGG AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT
    CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC GTGAGCACGG CGGGTCAGCG GCGCGGCCAG
    CGCATCGCCA TGCCGGTGCG CAAGTTCTTT GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC
    ATGGTGGGCC GGCTGACCAA CCGCACGTAC CGCAAGCGCA TCGACAGCTA CGTCAAACGC
    CAGATCGAGG ACATGGATGA CCACAGGCCG TTCTTCACCT ACTGGCTCAC CTTCGTGCAC
    TCGCTCGTCA CCATTCTGGC CGTGTGCATC TACGGTATCG CACCTGTGGG CTTCTCGCAG
    CACGAGACCG TGGACTCGGT GCTGCGGAAC CGCGGGGTCT ATGAGAATGT CAAGTATGTG
    CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC TCGGAGGCTC TCATTCACCT GGGTGCCAAG
    TTCTCGCCCT GCATGCGTCA GGACCCGCAG GTGCACAGTT TCATCCGTGC TGCGCGGGAG
    CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC AATGACCGGT CGGGCTGTGT GCAGACATCG
    GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC
    CCAGACCTTG CTGGCCACAA GAGGCAGTTT GGCTCTGTCT GCCACCAGGA CCCCAGGGTG
    TGTGATGAGC CCTCCTCAGA GGACCCCCAC GAGTGGCCAG ATGACATCAC CAAGTGGCCG
    ATCTGCACCA AAAACAGCGC CGGGAACCAC ACCAACCACC CGCACATGGA CTGTGTCCTC
    ACAGGCCGGC CCTGCTGCAT TGGCACCAAG GGCAGGTGTG AGATCACCTC CCGGGAGTAC
    TGTGACTTCA TGAGGGGCTA CTTCCACGAG GAGGCCACAC TGTGCTCCCA GGTGCATTGC
    ATGGATGACG TGTGTGGGCT CCTGCCCTTC CTGAACCCTG AGGTGCCCGA CCAGTTCTAC
    CGCCTGTGGC TGTCCCTCTT CTTGCACGCT GGGATCCTGC ACTGCCTGGT GTCCGTCTGC
    TTCCAGATGA CCGTCCTGCG GGACCTGGAG AAGTTGGCAG GCTGGCACCG CATAGCCATC
    ATCTACCTGT TGAGTGGTGT CACTGGTAAC CTGGCCAGTG CCATTTTCCT GCCATACCGG
    GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT GGCATCCTGG CTTGCCTGTT CGTGGAGCTT
    TTCCAGAGCT GGCAGGTCCT GGCACGGCCC TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG
    GTCCTCTTCC TGTTCACCTT CGGGCTGCTG CCCTGGATCG ACAACTTCGC TCACATCTCA
    GGCTTCATCA GCGGCCTCTT CCTGTCCTTC GCCTTCCTGC CCTACATCAG CTTCGGCAAG
    TTTGACTTGT ACCGCAAGCG CTGCCAGATC ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC
    CTGGCCGGCC TGGTGATCCT CTTCTACTTC TACCCCGTGC GCTGCGAGTG GTGCGAGTTC
    CTCACCTGCA TCCCCTTCAC CGACAAGTTC TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC
    CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08414
    Clone ID Related Accession (Same CDS sequence) XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
    Accession Version XM_005621771.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive rhomboid protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621828.1
    CDS371..3046
    Translation

    Target ORF information:

    RefSeq Version XM_005621771.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621771.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08414
    Clone ID Related Accession (Same CDS sequence) XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
    Accession Version XM_005621771.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive rhomboid protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621828.1
    CDS53..2728
    Translation

    Target ORF information:

    RefSeq Version XM_005621771.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621771.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08414
    Clone ID Related Accession (Same CDS sequence) XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
    Accession Version XM_005621773.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive rhomboid protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005621773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621830.1
    CDS102..2777
    Translation

    Target ORF information:

    RefSeq Version XM_005621773.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621773.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08414
    Clone ID Related Accession (Same CDS sequence) XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
    Accession Version XM_005621773.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive rhomboid protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005621773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621830.1
    CDS44..2719
    Translation

    Target ORF information:

    RefSeq Version XM_005621773.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621773.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08414
    Clone ID Related Accession (Same CDS sequence) XM_005621771.2 , XM_005621771.3 , XM_005621773.2 , XM_005621773.3 , XM_005621772.1
    Accession Version XM_005621772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive rhomboid protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005621829.1
    CDS60..2735
    Translation

    Target ORF information:

    RefSeq Version XM_005621772.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005621772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGCC GACAGGTGCG CTTTGGGCGT GTCCACACTC TGCCCCTCTT GGGACCCCCT
    GCTGCCCGTA GGGCTCTCCC CCAGCGCCGG TCTCTCTCTC GGTCCCTGCT CAGGGGCACA
    GCTGACTGGT TTGGAGTGAG CAAGGACAGT GACAGCACCC AGAAGTGGCA GCGCAAGAGC
    ATCCGCCACT GTAGCCAGCG CTATGGAAAG CTGAAGCCTC AGGTCATCCG GGAGCTAGAC
    CTGCCTAGCC AGGACAACGT GTCCCTGACC AGTACTGAGA CCCCCCCTCC ACTGTATGTG
    GGGCCGTGCC AGCTGGGCAT GCAGAAGATC ATAGACCCCC TGGCCCGGGG CCGGGCCTTC
    CGCGTGGCGG ATGACACGGC CGATGGCCTG AGTACCCCGC ACACACCTGT CACGCCTGGT
    GCCGCTTCCC TCTGCTCCTT CTCCAGCTCC CGCTCGGGCT TCAACCGGTT CCCGCGGCGA
    CGCAAGCGTG AATCGGTGGC CAAGATGAGC TTCCGGGCAG CCGCGGCCCT AGTGAAGGGC
    CGCTCTGTTA GGGATGGCAC GTTGCGCCGG GTGCAGCGCC GAAGCTTCAC TCCCGCCAGC
    TTCCTGGAGG AGGACACAGC TGACTTCCCC GACGAGCTTG ACACGTCCTT CTTCGCCCGG
    GAAGGAGTCC TCCATGAAGA GCTGTCCACG TACCCAGACG AGGTGTTTGA GTCCCCCTCG
    GAGGCAGCGC TCAAGGACTG GGAGAAAGCC ACGGAGCAGG GCGACCTCAC GGGGGGCGCC
    CTGGACCGCA GCGAGCTTGA GCGCAGCCAC CTGATGCTGC CCCTGGAACG AGGCTGGAGG
    AAGCAGAAGG AGGGTGGAGC AGCTGTCCCT CAGCCTAAGG TGCGGCTGCG GCAGGAGGTC
    GTGAGCACGG CGGGTCAGCG GCGCGGCCAG CGCATCGCCA TGCCGGTGCG CAAGTTCTTT
    GCCAGGGAGA AGCGGCCGTA TGGGCTAGGC ATGGTGGGCC GGCTGACCAA CCGCACGTAC
    CGCAAGCGCA TCGACAGCTA CGTCAAACGC CAGATCGAGG ACATGGATGA CCACAGGCCG
    TTCTTCACCT ACTGGCTCAC CTTCGTGCAC TCGCTCGTCA CCATTCTGGC CGTGTGCATC
    TACGGTATCG CACCTGTGGG CTTCTCGCAG CACGAGACCG TGGACTCGGT GCTGCGGAAC
    CGCGGGGTCT ATGAGAATGT CAAGTATGTG CAGCAGGAGA ACTTCTGGAT CGGGCCCAGC
    TCGGAGGCTC TCATTCACCT GGGTGCCAAG TTCTCGCCCT GCATGCGTCA GGACCCGCAG
    GTGCACAGTT TCATCCGTGC TGCGCGGGAG CGTGAGAAGC ACTCAGCCTG CTGCGTGCGC
    AATGACCGGT CGGGCTGTGT GCAGACATCG GAGGAGGAGT GCTCGTCCAC GCTGGCTGTG
    TGGGTGAAGT GGCCCTTGCA CCCCAGTGCC CCAGACCTTG CTGGCCACAA GAGGCAGTTT
    GGCTCTGTCT GCCACCAGGA CCCCAGGGTG TGTGATGAGC CCTCCTCAGA GGACCCCCAC
    GAGTGGCCAG ATGACATCAC CAAGTGGCCG ATCTGCACCA AAAACAGCGC CGGGAACCAC
    ACCAACCACC CGCACATGGA CTGTGTCCTC ACAGGCCGGC CCTGCTGCAT TGGCACCAAG
    GGCAGGTGTG AGATCACCTC CCGGGAGTAC TGTGACTTCA TGAGGGGCTA CTTCCACGAG
    GAGGCCACAC TGTGCTCCCA GGTGCATTGC ATGGATGACG TGTGTGGGCT CCTGCCCTTC
    CTGAACCCTG AGGTGCCCGA CCAGTTCTAC CGCCTGTGGC TGTCCCTCTT CTTGCACGCT
    GGGATCCTGC ACTGCCTGGT GTCCGTCTGC TTCCAGATGA CCGTCCTGCG GGACCTGGAG
    AAGTTGGCAG GCTGGCACCG CATAGCCATC ATCTACCTGT TGAGTGGTGT CACTGGTAAC
    CTGGCCAGTG CCATTTTCCT GCCATACCGG GCAGAGGTGG GCCCAGCCGG CTCGCAGTTT
    GGCATCCTGG CTTGCCTGTT CGTGGAGCTT TTCCAGAGCT GGCAGGTCCT GGCACGGCCC
    TGGCGTGCCT TCTTCAAGCT GTCGGCTGTG GTCCTCTTCC TGTTCACCTT CGGGCTGCTG
    CCCTGGATCG ACAACTTCGC TCACATCTCA GGCTTCATCA GCGGCCTCTT CCTGTCCTTC
    GCCTTCCTGC CCTACATCAG CTTCGGCAAG TTTGACTTGT ACCGCAAGCG CTGCCAGATC
    ATCGTCTTCC AGGTGGTCTT CCTGGGCCTC CTGGCCGGCC TGGTGATCCT CTTCTACTTC
    TACCCCGTGC GCTGCGAGTG GTGCGAGTTC CTCACCTGCA TCCCCTTCAC CGACAAGTTC
    TGCGAGAAGT ACGAGCTGGA TGCCCAGCTC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08416
    Clone ID Related Accession (Same CDS sequence) XM_547222.6 , XM_547222.5
    Accession Version XM_547222.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive rhomboid protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_547222.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG
    TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC
    ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC
    CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA
    CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC
    CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG
    GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC
    TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG
    TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG
    TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC
    CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG
    GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG
    CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG
    GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG
    ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG
    GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT
    CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC
    TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC
    TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG
    CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA
    GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG
    TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC
    CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC
    TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC
    AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC
    TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG
    GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT
    GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC
    CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC
    CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT
    GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA
    GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG
    GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC
    ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC
    CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC
    AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG
    ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC
    TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_547222.1
    CDS119..2689
    Translation

    Target ORF information:

    RefSeq Version XM_547222.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_547222.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG
    TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC
    ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC
    CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA
    CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC
    CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG
    GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC
    TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG
    TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG
    TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC
    CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG
    GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG
    CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG
    GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG
    ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG
    GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT
    CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC
    TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC
    TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG
    CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA
    GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG
    TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC
    CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC
    TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC
    AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC
    TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG
    GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT
    GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC
    CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC
    CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT
    GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA
    GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG
    GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC
    ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC
    CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC
    AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG
    ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC
    TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08416
    Clone ID Related Accession (Same CDS sequence) XM_547222.6 , XM_547222.5
    Accession Version XM_547222.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product inactive rhomboid protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726065.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_547222.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG
    TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC
    ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC
    CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA
    CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC
    CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG
    GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC
    TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG
    TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG
    TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC
    CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG
    GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG
    CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG
    GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG
    ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG
    GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT
    CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC
    TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC
    TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG
    CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA
    GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG
    TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC
    CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC
    TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC
    AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC
    TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG
    GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT
    GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC
    CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC
    CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT
    GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA
    GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG
    GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC
    ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC
    CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC
    AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG
    ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC
    TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_547222.1
    CDS189..2759
    Translation

    Target ORF information:

    RefSeq Version XM_547222.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (Drosophila) (RHBDF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_547222.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG 
    AAGCTGGACA TCCCGGCCGC AGCACCCCCG GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC
    CTGAGACGCC AGGCCTTCCT GCGGAGCGTG AGTATGCCGG CAGAGACCGC CCCCGTGCCC
    TCACCCCACC CGGAGCTCCG GCGGCTGGGG CTGCAGCGCC AGACGTCCAT CACGCAGACC
    ATCCGCAGGG GCACAGCTGA CTGGTTTGGA GTGAGCAAGG ACAGTGACAG CACCCAGAAG
    TGGCAGCGCA AGAGCATCCG CCACTGTAGC CAGCGCTATG GAAAGCTGAA GCCTCAGGTC
    ATCCGGGAGC TAGACCTGCC TAGCCAGGAC AACGTGTCCC TGACCAGTAC TGAGACCCCC
    CCTCCACTGT ATGTGGGGCC GTGCCAGCTG GGCATGCAGA AGATCATAGA CCCCCTGGCC
    CGGGGCCGGG CCTTCCGCGT GGCGGATGAC ACGGCCGATG GCCTGAGTAC CCCGCACACA
    CCTGTCACGC CTGGTGCCGC TTCCCTCTGC TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC
    CGGTTCCCGC GGCGACGCAA GCGTGAATCG GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG
    GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT GGCACGTTGC GCCGGGTGCA GCGCCGAAGC
    TTCACTCCCG CCAGCTTCCT GGAGGAGGAC ACAGCTGACT TCCCCGACGA GCTTGACACG
    TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT GAAGAGCTGT CCACGTACCC AGACGAGGTG
    TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG GACTGGGAGA AAGCCACGGA GCAGGGCGAC
    CTCACGGGGG GCGCCCTGGA CCGCAGCGAG CTTGAGCGCA GCCACCTGAT GCTGCCCCTG
    GAACGAGGCT GGAGGAAGCA GAAGGAGGGT GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG
    CTGCGGCAGG AGGTCGTGAG CACGGCGGGT CAGCGGCGCG GCCAGCGCAT CGCCATGCCG
    GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG CCGTATGGGC TAGGCATGGT GGGCCGGCTG
    ACCAACCGCA CGTACCGCAA GCGCATCGAC AGCTACGTCA AACGCCAGAT CGAGGACATG
    GATGACCACA GGCCGTTCTT CACCTACTGG CTCACCTTCG TGCACTCGCT CGTCACCATT
    CTGGCCGTGT GCATCTACGG TATCGCACCT GTGGGCTTCT CGCAGCACGA GACCGTGGAC
    TCGGTGCTGC GGAACCGCGG GGTCTATGAG AATGTCAAGT ATGTGCAGCA GGAGAACTTC
    TGGATCGGGC CCAGCTCGGA GGCTCTCATT CACCTGGGTG CCAAGTTCTC GCCCTGCATG
    CGTCAGGACC CGCAGGTGCA CAGTTTCATC CGTGCTGCGC GGGAGCGTGA GAAGCACTCA
    GCCTGCTGCG TGCGCAATGA CCGGTCGGGC TGTGTGCAGA CATCGGAGGA GGAGTGCTCG
    TCCACGCTGG CTGTGTGGGT GAAGTGGCCC TTGCACCCCA GTGCCCCAGA CCTTGCTGGC
    CACAAGAGGC AGTTTGGCTC TGTCTGCCAC CAGGACCCCA GGGTGTGTGA TGAGCCCTCC
    TCAGAGGACC CCCACGAGTG GCCAGATGAC ATCACCAAGT GGCCGATCTG CACCAAAAAC
    AGCGCCGGGA ACCACACCAA CCACCCGCAC ATGGACTGTG TCCTCACAGG CCGGCCCTGC
    TGCATTGGCA CCAAGGGCAG GTGTGAGATC ACCTCCCGGG AGTACTGTGA CTTCATGAGG
    GGCTACTTCC ACGAGGAGGC CACACTGTGC TCCCAGGTGC ATTGCATGGA TGACGTGTGT
    GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG CCCGACCAGT TCTACCGCCT GTGGCTGTCC
    CTCTTCTTGC ACGCTGGGAT CCTGCACTGC CTGGTGTCCG TCTGCTTCCA GATGACCGTC
    CTGCGGGACC TGGAGAAGTT GGCAGGCTGG CACCGCATAG CCATCATCTA CCTGTTGAGT
    GGTGTCACTG GTAACCTGGC CAGTGCCATT TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA
    GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG
    GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC
    ACCTTCGGGC TGCTGCCCTG GATCGACAAC TTCGCTCACA TCTCAGGCTT CATCAGCGGC
    CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC ATCAGCTTCG GCAAGTTTGA CTTGTACCGC
    AAGCGCTGCC AGATCATCGT CTTCCAGGTG GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG
    ATCCTCTTCT ACTTCTACCC CGTGCGCTGC GAGTGGTGCG AGTTCCTCAC CTGCATCCCC
    TTCACCGACA AGTTCTGCGA GAAGTACGAG CTGGATGCCC AGCTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe08415
    Clone ID Related Accession (Same CDS sequence) XM_022420328.1
    Accession Version XM_022420328.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product inactive rhomboid protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006588.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGGG GCACAGCTGA CTGGTTTGGA
    GTGAGCAAGG ACAGTGACAG CACCCAGAAG TGGCAGCGCA AGAGCATCCG CCACTGTAGC
    CAGCGCTATG GAAAGCTGAA GCCTCAGGTC ATCCGGGAGC TAGACCTGCC TAGCCAGGAC
    AACGTGTCCC TGACCAGTAC TGAGACCCCC CCTCCACTGT ATGTGGGGCC GTGCCAGCTG
    GGCATGCAGA AGATCATAGA CCCCCTGGCC CGGGGCCGGG CCTTCCGCGT GGCGGATGAC
    ACGGCCGATG GCCTGAGTAC CCCGCACACA CCTGTCACGC CTGGTGCCGC TTCCCTCTGC
    TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC CGGTTCCCGC GGCGACGCAA GCGTGAATCG
    GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT
    GGCACGTTGC GCCGGGTGCA GCGCCGAAGC TTCACTCCCG CCAGCTTCCT GGAGGAGGAC
    ACAGCTGACT TCCCCGACGA GCTTGACACG TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT
    GAAGAGCTGT CCACGTACCC AGACGAGGTG TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG
    GACTGGGAGA AAGCCACGGA GCAGGGCGAC CTCACGGGGG GCGCCCTGGA CCGCAGCGAG
    CTTGAGCGCA GCCACCTGAT GCTGCCCCTG GAACGAGGCT GGAGGAAGCA GAAGGAGGGT
    GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG CTGCGGCAGG AGGTCGTGAG CACGGCGGGT
    CAGCGGCGCG GCCAGCGCAT CGCCATGCCG GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG
    CCGTATGGGC TAGGCATGGT GGGCCGGCTG ACCAACCGCA CGTACCGCAA GCGCATCGAC
    AGCTACGTCA AACGCCAGAT CGAGGACATG GATGACCACA GGCCGTTCTT CACCTACTGG
    CTCACCTTCG TGCACTCGCT CGTCACCATT CTGGCCGTGT GCATCTACGG TATCGCACCT
    GTGGGCTTCT CGCAGCACGA GACCGTGGAC TCGGTGCTGC GGAACCGCGG GGTCTATGAG
    AATGTCAAGT ATGTGCAGCA GGAGAACTTC TGGATCGGGC CCAGCTCGGA GGCTCTCATT
    CACCTGGGTG CCAAGTTCTC GCCCTGCATG CGTCAGGACC CGCAGGTGCA CAGTTTCATC
    CGTGCTGCGC GGGAGCGTGA GAAGCACTCA GCCTGCTGCG TGCGCAATGA CCGGTCGGGC
    TGTGTGCAGA CATCGGAGGA GGAGTGCTCG TCCACGCTGG CTGTGTGGGT GAAGTGGCCC
    TTGCACCCCA GTGCCCCAGA CCTTGCTGGC CACAAGAGGC AGTTTGGCTC TGTCTGCCAC
    CAGGACCCCA GGGTGTGTGA TGAGCCCTCC TCAGAGGACC CCCACGAGTG GCCAGATGAC
    ATCACCAAGT GGCCGATCTG CACCAAAAAC AGCGCCGGGA ACCACACCAA CCACCCGCAC
    ATGGACTGTG TCCTCACAGG CCGGCCCTGC TGCATTGGCA CCAAGGGCAG GTGTGAGATC
    ACCTCCCGGG AGTACTGTGA CTTCATGAGG GGCTACTTCC ACGAGGAGGC CACACTGTGC
    TCCCAGGTGC ATTGCATGGA TGACGTGTGT GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG
    CCCGACCAGT TCTACCGCCT GTGGCTGTCC CTCTTCTTGC ACGCTGGGAT CCTGCACTGC
    CTGGTGTCCG TCTGCTTCCA GATGACCGTC CTGCGGGACC TGGAGAAGTT GGCAGGCTGG
    CACCGCATAG CCATCATCTA CCTGTTGAGT GGTGTCACTG GTAACCTGGC CAGTGCCATT
    TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC
    CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC
    AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC ACCTTCGGGC TGCTGCCCTG GATCGACAAC
    TTCGCTCACA TCTCAGGCTT CATCAGCGGC CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC
    ATCAGCTTCG GCAAGTTTGA CTTGTACCGC AAGCGCTGCC AGATCATCGT CTTCCAGGTG
    GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG ATCCTCTTCT ACTTCTACCC CGTGCGCTGC
    GAGTGGTGCG AGTTCCTCAC CTGCATCCCC TTCACCGACA AGTTCTGCGA GAAGTACGAG
    CTGGATGCCC AGCTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276036.1
    CDS334..2934
    Translation

    Target ORF information:

    RefSeq Version XM_022420328.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rhomboid 5 homolog 1 (RHBDF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGGCCC GCCCTGCCCC ACGCGGAACC ATGAGTGAGG CCCGCAGGGA CAGCGCGAGC 
    AGCCTGCAGC GCAAGAAGCC ACCCTGGCTG AAGCTGGACA TCCCGGCCGC AGCACCCCCG
    GCCCCGGAGG AGCCCAGCTG CTTGCAGCCC CTGAGACGCC AGGCCTTCCT GCGGAGCGTG
    AGTATGCCGG CAGAGACCGC CCCCGTGCCC TCACCCCACC CGGAGCTCCG GCGGCTGGGG
    CTGCAGCGCC AGACGTCCAT CACGCAGACC ATCCGCAGGG GCACAGCTGA CTGGTTTGGA
    GTGAGCAAGG ACAGTGACAG CACCCAGAAG TGGCAGCGCA AGAGCATCCG CCACTGTAGC
    CAGCGCTATG GAAAGCTGAA GCCTCAGGTC ATCCGGGAGC TAGACCTGCC TAGCCAGGAC
    AACGTGTCCC TGACCAGTAC TGAGACCCCC CCTCCACTGT ATGTGGGGCC GTGCCAGCTG
    GGCATGCAGA AGATCATAGA CCCCCTGGCC CGGGGCCGGG CCTTCCGCGT GGCGGATGAC
    ACGGCCGATG GCCTGAGTAC CCCGCACACA CCTGTCACGC CTGGTGCCGC TTCCCTCTGC
    TCCTTCTCCA GCTCCCGCTC GGGCTTCAAC CGGTTCCCGC GGCGACGCAA GCGTGAATCG
    GTGGCCAAGA TGAGCTTCCG GGCAGCCGCG GCCCTAGTGA AGGGCCGCTC TGTTAGGGAT
    GGCACGTTGC GCCGGGTGCA GCGCCGAAGC TTCACTCCCG CCAGCTTCCT GGAGGAGGAC
    ACAGCTGACT TCCCCGACGA GCTTGACACG TCCTTCTTCG CCCGGGAAGG AGTCCTCCAT
    GAAGAGCTGT CCACGTACCC AGACGAGGTG TTTGAGTCCC CCTCGGAGGC AGCGCTCAAG
    GACTGGGAGA AAGCCACGGA GCAGGGCGAC CTCACGGGGG GCGCCCTGGA CCGCAGCGAG
    CTTGAGCGCA GCCACCTGAT GCTGCCCCTG GAACGAGGCT GGAGGAAGCA GAAGGAGGGT
    GGAGCAGCTG TCCCTCAGCC TAAGGTGCGG CTGCGGCAGG AGGTCGTGAG CACGGCGGGT
    CAGCGGCGCG GCCAGCGCAT CGCCATGCCG GTGCGCAAGT TCTTTGCCAG GGAGAAGCGG
    CCGTATGGGC TAGGCATGGT GGGCCGGCTG ACCAACCGCA CGTACCGCAA GCGCATCGAC
    AGCTACGTCA AACGCCAGAT CGAGGACATG GATGACCACA GGCCGTTCTT CACCTACTGG
    CTCACCTTCG TGCACTCGCT CGTCACCATT CTGGCCGTGT GCATCTACGG TATCGCACCT
    GTGGGCTTCT CGCAGCACGA GACCGTGGAC TCGGTGCTGC GGAACCGCGG GGTCTATGAG
    AATGTCAAGT ATGTGCAGCA GGAGAACTTC TGGATCGGGC CCAGCTCGGA GGCTCTCATT
    CACCTGGGTG CCAAGTTCTC GCCCTGCATG CGTCAGGACC CGCAGGTGCA CAGTTTCATC
    CGTGCTGCGC GGGAGCGTGA GAAGCACTCA GCCTGCTGCG TGCGCAATGA CCGGTCGGGC
    TGTGTGCAGA CATCGGAGGA GGAGTGCTCG TCCACGCTGG CTGTGTGGGT GAAGTGGCCC
    TTGCACCCCA GTGCCCCAGA CCTTGCTGGC CACAAGAGGC AGTTTGGCTC TGTCTGCCAC
    CAGGACCCCA GGGTGTGTGA TGAGCCCTCC TCAGAGGACC CCCACGAGTG GCCAGATGAC
    ATCACCAAGT GGCCGATCTG CACCAAAAAC AGCGCCGGGA ACCACACCAA CCACCCGCAC
    ATGGACTGTG TCCTCACAGG CCGGCCCTGC TGCATTGGCA CCAAGGGCAG GTGTGAGATC
    ACCTCCCGGG AGTACTGTGA CTTCATGAGG GGCTACTTCC ACGAGGAGGC CACACTGTGC
    TCCCAGGTGC ATTGCATGGA TGACGTGTGT GGGCTCCTGC CCTTCCTGAA CCCTGAGGTG
    CCCGACCAGT TCTACCGCCT GTGGCTGTCC CTCTTCTTGC ACGCTGGGAT CCTGCACTGC
    CTGGTGTCCG TCTGCTTCCA GATGACCGTC CTGCGGGACC TGGAGAAGTT GGCAGGCTGG
    CACCGCATAG CCATCATCTA CCTGTTGAGT GGTGTCACTG GTAACCTGGC CAGTGCCATT
    TTCCTGCCAT ACCGGGCAGA GGTGGGCCCA GCCGGCTCGC AGTTTGGCAT CCTGGCTTGC
    CTGTTCGTGG AGCTTTTCCA GAGCTGGCAG GTCCTGGCAC GGCCCTGGCG TGCCTTCTTC
    AAGCTGTCGG CTGTGGTCCT CTTCCTGTTC ACCTTCGGGC TGCTGCCCTG GATCGACAAC
    TTCGCTCACA TCTCAGGCTT CATCAGCGGC CTCTTCCTGT CCTTCGCCTT CCTGCCCTAC
    ATCAGCTTCG GCAAGTTTGA CTTGTACCGC AAGCGCTGCC AGATCATCGT CTTCCAGGTG
    GTCTTCCTGG GCCTCCTGGC CGGCCTGGTG ATCCTCTTCT ACTTCTACCC CGTGCGCTGC
    GAGTGGTGCG AGTTCCTCAC CTGCATCCCC TTCACCGACA AGTTCTGCGA GAAGTACGAG
    CTGGATGCCC AGCTCCACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.