VAC14 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAC14
Entrez Gene ID	489717
Full Name	Vac14, PIKFYVE complex component
General protein information	Preferred Names Vac14, PIKFYVE complex component Names protein VAC14 homolog
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_546837.6	XP_546837.2	protein VAC14 homolog
XM_546837.5	XP_546837.2	protein VAC14 homolog

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Vac14	NP_808791.1
Canis lupus familiaris (dog)	VAC14	XP_546837.2
Bos taurus (cattle)	VAC14	NP_001075913.1
Danio rerio (zebrafish)	vac14	NP_001004650.1
Kluyveromyces lactis	KLLA0E05193g	XP_002999395.1
Eremothecium gossypii	AGOS_AER049W	NP_984909.2
Neurospora crassa	NCU02409	XP_959537.2
Oryza sativa (rice)	Os03g0223700	NP_001049425.1
Mus musculus (house mouse)	Vac14	NP_666328.2
Drosophila melanogaster (fruit fly)	CG5608	NP_650208.1
Arabidopsis thaliana (thale cress)	AT2G01690	NP_973395.1
Pan troglodytes (chimpanzee)	VAC14	XP_001170940.1
Gallus gallus (chicken)	VAC14	NP_001025735.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000770	XP_311309.5
Caenorhabditis elegans (roundworm)	K04G2.6	NP_492215.1
Schizosaccharomyces pombe (fission yeast)	SPBC25H2.03	NP_596363.1
Magnaporthe oryzae (rice blast fungus)	MGG_03568	XP_003716347.1
Homo sapiens (human)	VAC14	NP_060522.3
	VAC14	XP_001106962.1
Saccharomyces cerevisiae (baker's yeast)	VAC14	NP_013490.3
Xenopus tropicalis (tropical clawed frog)	vac14	NP_001135664.1

Gene Ontology

The following VAC14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAC14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe07138	XM_546837.6 Latest version!	Canis lupus familiaris Vac14, PIKFYVE complex component (VAC14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
OCe07138	XM_546837.5	Canis lupus familiaris Vac14 homolog (S. cerevisiae) (VAC14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAC14 ( XM_546837.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_546837.2 Canis lupus familiaris Vac14, PIKFYVE complex component (VAC14), mRNA.

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CloneID	OCe07138
Clone ID Related Accession (Same CDS sequence)	XM_546837.6 , XM_546837.5
Accession Version	XM_546837.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein VAC14 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546837.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCGG AGAAGGACTT CGCGCCGCTC ACGCCCAACA TCGTGCGCGC CCTCAATGAC 
AAGCTCTACG AAAAGCGGAA GGTGGCAGCG CTGGAGATTG AGAAGCTGGT CCGGGAGTTC 
GTGGCCCAGA ACAACACCGT GCAAATCAAG CATGTGATCC AGACCCTGTC TCAGGAGTTC 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAG GGGGGCCTCA TCGGCCTGGC CGCCTGCTCT 
ATTGCACTGG GCAAGGACTC AGGGCTGTAC CTAAAGGAGC TCATTGAGCC GGTGCTGACC 
TGCTTCAATG ATGCAGACAG CAGGCTGCGC TACTACGCAT GCGAGGCACT CTACAACATC 
GTCAAGGTGG CCCGTGGCGC CGTGCTGCCT CATTTCAACG TGCTCTTCGA CGGGCTCAGC 
AAGCTGGCGG CGGACCCAGA CCCTAATGTA AAAAGTGGAT CTGAGCTCCT AGACCGCCTT 
TTAAAGGACA TTGTGACAGA GAGCAACAAG TTTGATCTGG TGGGCTTCAT CCCTTTGTTG 
CGGGAGAGGA TTTATTCCAA CAACCAGTAC GCCCGGCAGT TCATCATCTC CTGGATCCTG 
GTTCTGGAGT CCGTGCCAGA CATTAACCTG CTGGATTATT TGCCGGAGAT CCTGGATGGA 
CTCTTCCAGA TCCTGGGCGA CAACGGCAAA GAGATTCGGA AAATGTGTGA GGTAGTCCTT 
GGGGAATTCT TGAAAGAAAT CAAGAAGAAC CCCTCCAGTG TTAAGTTTGC TGAAATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TCCAGCTGAC AGCCATGTGC 
TGGATGCGAG AGTTCATCCA GCTGGCTGGC CGAGTGATGT TGCCCTACTC ATCTGGAATC 
CTGACCGCTG TCTTGCCCTG CCTGGCCTAC GATGACCGCA AGAAAAGCAT CAAGGAGGTG 
GCCAACGTGT GTAACCAAAG CCTGATGAAG CTGGTTACCC CTGAGGATGA TGAGCCTGAC 
GAGCTGAAGC CAGTGGTACA GAGGCAGGCA GAGCCCAATC CCGATGACTC CGTGGCCAAG 
CAGGAAGGGA CAGCCACTGG AGGCCCCGAT GGTTCTTGTG ACTCAAGCTT CAGTAGCAGC 
ATCAGCGTCT TCACGCCAGC CAGCACTGAA AGGGCCCCAG TGACCCTCCA CCTCGATGGG 
ATTGTGCAGG TCCTGAACTG CCACCTCAGT GACATGGCCA TCGGGATGAT GACCAGGATT 
GCTGTCCTGA AGTGGCTCTA CCATCTGTAT ATCAAGACTC CCCGGAAGAT GTTCCGGCAC 
ACGGACAGCC TCTTCCCCAT CCTACTGCAG ACGTTATCGG ACGAATCTGA TGAGGTTATC 
CTGAAGGATC TAGAGGTATT GGCAGAAATT GCCTCCTCCC CTGCAGGCCA GACGGATGAC 
CCAGGCCCCC TTGATGGCCC TGACTTCCGG GTCAGCCACT CAGAGCTTCA GGTGCCCACC 
CCTGGCAGAG CCAACCTACT GAATACTCCT GGTACCAAAG GCTTAGAATG TTCTCCTTCC 
ACCCCCACCA TGAACTCCTA CTTTTATAAG TTCATGATCA ACCTTCTCAA GAGGTTCAGC 
AGTGAACGCA AGCTCCTGGA GGCCAGAGGC GCTTTCATCA TCAGGCAGCT CTGCCTCCTG 
CTGAATGCAG AGAACATCTT TCACTCGATG GCAGACATCC TGCTCCGAGA GGAGGACCTC 
AAGTTCGCCT CAACCATGGT CCACACGCTC AACACCATCC TGCTCACCTC CACAGAGCTC 
TTCCAGCTGA GGAACCAGCT CAAGGACCTG AAGACTCTGG AGAGCCAGAA CCTGTTCTGC 
TGCCTGTACC GCTCCTGGTG CCACAACCCA GTCACAACAG TGTCCCTGTG CTTCCTCACC 
CAGAACTACC GGCACGCCTA CGACCTCATC CAGAAGTTCG GGGACCTGGA GGTCACGGTG 
GATTTCCTCA CTGAGGTGGA CAAGCTGGTG CAGCTGATCG AGTGCCCCAT CTTCACATAC 
CTGCGCCTGC AGCTGCTCGA CGTGAAGAAC AACCCGTACC TGATCAAGGC CCTCTACGGC 
CTGCTCATGC TGCTGCCCCA GAGCGGAGCC TTCCAGCTGC TCTCCCACCG GCTGCAGTGT 
GTGCCCAACC CCGAGCTGCT ACAGACCGAA GACAGTCCGC AGGCCACCCC CAAGTCACAG 
AAAGCAGATT CCCCCAGCAT CGACTATGCA GAGCTGTTGC GGCACTTTGA GAAGGTCCAG 
AAGAAGCACC TGGAAGTGAG ACACCAGCGG AGTGGGCGCG GGGACCACCT GGACCGAAGG 
GTTGTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_546837.2
CDS	288..2639
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVREFVAQNNTVQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESNKFDLVGFIPLLRERIYSNNQYARQFIISWILVLESVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDELKPVVQRQAEPNPDDSVAKQEGTATGGPDGSCDSSFSSSISVFTPASTERAPVTLHLDGIVQVLNCHLSDMAIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQTDDPGPLDGPDFRVSHSELQVPTPGRANLLNTPGTKGLECSPSTPTMNSYFYKFMINLLKRFSSERKLLEARGAFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSGAFQLLSHRLQCVPNPELLQTEDSPQATPKSQKADSPSIDYAELLRHFEKVQKKHLEVRHQRSGRGDHLDRRVVL

Target ORF information:

RefSeq Version	XM_546837.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Vac14, PIKFYVE complex component (VAC14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_546837.6

ORF Insert Sequence:

ATGAACCCGG AGAAGGACTT CGCGCCGCTC ACGCCCAACA TCGTGCGCGC CCTCAATGAC 
AAGCTCTACG AAAAGCGGAA GGTGGCAGCG CTGGAGATTG AGAAGCTGGT CCGGGAGTTC 
GTGGCCCAGA ACAACACCGT GCAAATCAAG CATGTGATCC AGACCCTGTC TCAGGAGTTC 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAG GGGGGCCTCA TCGGCCTGGC CGCCTGCTCT 
ATTGCACTGG GCAAGGACTC AGGGCTGTAC CTAAAGGAGC TCATTGAGCC GGTGCTGACC 
TGCTTCAATG ATGCAGACAG CAGGCTGCGC TACTACGCAT GCGAGGCACT CTACAACATC 
GTCAAGGTGG CCCGTGGCGC CGTGCTGCCT CATTTCAACG TGCTCTTCGA CGGGCTCAGC 
AAGCTGGCGG CGGACCCAGA CCCTAATGTA AAAAGTGGAT CTGAGCTCCT AGACCGCCTT 
TTAAAGGACA TTGTGACAGA GAGCAACAAG TTTGATCTGG TGGGCTTCAT CCCTTTGTTG 
CGGGAGAGGA TTTATTCCAA CAACCAGTAC GCCCGGCAGT TCATCATCTC CTGGATCCTG 
GTTCTGGAGT CCGTGCCAGA CATTAACCTG CTGGATTATT TGCCGGAGAT CCTGGATGGA 
CTCTTCCAGA TCCTGGGCGA CAACGGCAAA GAGATTCGGA AAATGTGTGA GGTAGTCCTT 
GGGGAATTCT TGAAAGAAAT CAAGAAGAAC CCCTCCAGTG TTAAGTTTGC TGAAATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TCCAGCTGAC AGCCATGTGC 
TGGATGCGAG AGTTCATCCA GCTGGCTGGC CGAGTGATGT TGCCCTACTC ATCTGGAATC 
CTGACCGCTG TCTTGCCCTG CCTGGCCTAC GATGACCGCA AGAAAAGCAT CAAGGAGGTG 
GCCAACGTGT GTAACCAAAG CCTGATGAAG CTGGTTACCC CTGAGGATGA TGAGCCTGAC 
GAGCTGAAGC CAGTGGTACA GAGGCAGGCA GAGCCCAATC CCGATGACTC CGTGGCCAAG 
CAGGAAGGGA CAGCCACTGG AGGCCCCGAT GGTTCTTGTG ACTCAAGCTT CAGTAGCAGC 
ATCAGCGTCT TCACGCCAGC CAGCACTGAA AGGGCCCCAG TGACCCTCCA CCTCGATGGG 
ATTGTGCAGG TCCTGAACTG CCACCTCAGT GACATGGCCA TCGGGATGAT GACCAGGATT 
GCTGTCCTGA AGTGGCTCTA CCATCTGTAT ATCAAGACTC CCCGGAAGAT GTTCCGGCAC 
ACGGACAGCC TCTTCCCCAT CCTACTGCAG ACGTTATCGG ACGAATCTGA TGAGGTTATC 
CTGAAGGATC TAGAGGTATT GGCAGAAATT GCCTCCTCCC CTGCAGGCCA GACGGATGAC 
CCAGGCCCCC TTGATGGCCC TGACTTCCGG GTCAGCCACT CAGAGCTTCA GGTGCCCACC 
CCTGGCAGAG CCAACCTACT GAATACTCCT GGTACCAAAG GCTTAGAATG TTCTCCTTCC 
ACCCCCACCA TGAACTCCTA CTTTTATAAG TTCATGATCA ACCTTCTCAA GAGGTTCAGC 
AGTGAACGCA AGCTCCTGGA GGCCAGAGGC GCTTTCATCA TCAGGCAGCT CTGCCTCCTG 
CTGAATGCAG AGAACATCTT TCACTCGATG GCAGACATCC TGCTCCGAGA GGAGGACCTC 
AAGTTCGCCT CAACCATGGT CCACACGCTC AACACCATCC TGCTCACCTC CACAGAGCTC 
TTCCAGCTGA GGAACCAGCT CAAGGACCTG AAGACTCTGG AGAGCCAGAA CCTGTTCTGC 
TGCCTGTACC GCTCCTGGTG CCACAACCCA GTCACAACAG TGTCCCTGTG CTTCCTCACC 
CAGAACTACC GGCACGCCTA CGACCTCATC CAGAAGTTCG GGGACCTGGA GGTCACGGTG 
GATTTCCTCA CTGAGGTGGA CAAGCTGGTG CAGCTGATCG AGTGCCCCAT CTTCACATAC 
CTGCGCCTGC AGCTGCTCGA CGTGAAGAAC AACCCGTACC TGATCAAGGC CCTCTACGGC 
CTGCTCATGC TGCTGCCCCA GAGCGGAGCC TTCCAGCTGC TCTCCCACCG GCTGCAGTGT 
GTGCCCAACC CCGAGCTGCT ACAGACCGAA GACAGTCCGC AGGCCACCCC CAAGTCACAG 
AAAGCAGATT CCCCCAGCAT CGACTATGCA GAGCTGTTGC GGCACTTTGA GAAGGTCCAG 
AAGAAGCACC TGGAAGTGAG ACACCAGCGG AGTGGGCGCG GGGACCACCT GGACCGAAGG 
GTTGTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAC14 ( XM_546837.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_546837.2 Canis lupus familiaris Vac14 homolog (S. cerevisiae) (VAC14), mRNA.

Price & Availability↑

CloneID	OCe07138
Clone ID Related Accession (Same CDS sequence)	XM_546837.6 , XM_546837.5
Accession Version	XM_546837.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein VAC14 homolog
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546837.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCGG AGAAGGACTT CGCGCCGCTC ACGCCCAACA TCGTGCGCGC CCTCAATGAC 
AAGCTCTACG AAAAGCGGAA GGTGGCAGCG CTGGAGATTG AGAAGCTGGT CCGGGAGTTC 
GTGGCCCAGA ACAACACCGT GCAAATCAAG CATGTGATCC AGACCCTGTC TCAGGAGTTC 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAG GGGGGCCTCA TCGGCCTGGC CGCCTGCTCT 
ATTGCACTGG GCAAGGACTC AGGGCTGTAC CTAAAGGAGC TCATTGAGCC GGTGCTGACC 
TGCTTCAATG ATGCAGACAG CAGGCTGCGC TACTACGCAT GCGAGGCACT CTACAACATC 
GTCAAGGTGG CCCGTGGCGC CGTGCTGCCT CATTTCAACG TGCTCTTCGA CGGGCTCAGC 
AAGCTGGCGG CGGACCCAGA CCCTAATGTA AAAAGTGGAT CTGAGCTCCT AGACCGCCTT 
TTAAAGGACA TTGTGACAGA GAGCAACAAG TTTGATCTGG TGGGCTTCAT CCCTTTGTTG 
CGGGAGAGGA TTTATTCCAA CAACCAGTAC GCCCGGCAGT TCATCATCTC CTGGATCCTG 
GTTCTGGAGT CCGTGCCAGA CATTAACCTG CTGGATTATT TGCCGGAGAT CCTGGATGGA 
CTCTTCCAGA TCCTGGGCGA CAACGGCAAA GAGATTCGGA AAATGTGTGA GGTAGTCCTT 
GGGGAATTCT TGAAAGAAAT CAAGAAGAAC CCCTCCAGTG TTAAGTTTGC TGAAATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TCCAGCTGAC AGCCATGTGC 
TGGATGCGAG AGTTCATCCA GCTGGCTGGC CGAGTGATGT TGCCCTACTC ATCTGGAATC 
CTGACCGCTG TCTTGCCCTG CCTGGCCTAC GATGACCGCA AGAAAAGCAT CAAGGAGGTG 
GCCAACGTGT GTAACCAAAG CCTGATGAAG CTGGTTACCC CTGAGGATGA TGAGCCTGAC 
GAGCTGAAGC CAGTGGTACA GAGGCAGGCA GAGCCCAATC CCGATGACTC CGTGGCCAAG 
CAGGAAGGGA CAGCCACTGG AGGCCCCGAT GGTTCTTGTG ACTCAAGCTT CAGTAGCAGC 
ATCAGCGTCT TCACGCCAGC CAGCACTGAA AGGGCCCCAG TGACCCTCCA CCTCGATGGG 
ATTGTGCAGG TCCTGAACTG CCACCTCAGT GACATGGCCA TCGGGATGAT GACCAGGATT 
GCTGTCCTGA AGTGGCTCTA CCATCTGTAT ATCAAGACTC CCCGGAAGAT GTTCCGGCAC 
ACGGACAGCC TCTTCCCCAT CCTACTGCAG ACGTTATCGG ACGAATCTGA TGAGGTTATC 
CTGAAGGATC TAGAGGTATT GGCAGAAATT GCCTCCTCCC CTGCAGGCCA GACGGATGAC 
CCAGGCCCCC TTGATGGCCC TGACTTCCGG GTCAGCCACT CAGAGCTTCA GGTGCCCACC 
CCTGGCAGAG CCAACCTACT GAATACTCCT GGTACCAAAG GCTTAGAATG TTCTCCTTCC 
ACCCCCACCA TGAACTCCTA CTTTTATAAG TTCATGATCA ACCTTCTCAA GAGGTTCAGC 
AGTGAACGCA AGCTCCTGGA GGCCAGAGGC GCTTTCATCA TCAGGCAGCT CTGCCTCCTG 
CTGAATGCAG AGAACATCTT TCACTCGATG GCAGACATCC TGCTCCGAGA GGAGGACCTC 
AAGTTCGCCT CAACCATGGT CCACACGCTC AACACCATCC TGCTCACCTC CACAGAGCTC 
TTCCAGCTGA GGAACCAGCT CAAGGACCTG AAGACTCTGG AGAGCCAGAA CCTGTTCTGC 
TGCCTGTACC GCTCCTGGTG CCACAACCCA GTCACAACAG TGTCCCTGTG CTTCCTCACC 
CAGAACTACC GGCACGCCTA CGACCTCATC CAGAAGTTCG GGGACCTGGA GGTCACGGTG 
GATTTCCTCA CTGAGGTGGA CAAGCTGGTG CAGCTGATCG AGTGCCCCAT CTTCACATAC 
CTGCGCCTGC AGCTGCTCGA CGTGAAGAAC AACCCGTACC TGATCAAGGC CCTCTACGGC 
CTGCTCATGC TGCTGCCCCA GAGCGGAGCC TTCCAGCTGC TCTCCCACCG GCTGCAGTGT 
GTGCCCAACC CCGAGCTGCT ACAGACCGAA GACAGTCCGC AGGCCACCCC CAAGTCACAG 
AAAGCAGATT CCCCCAGCAT CGACTATGCA GAGCTGTTGC GGCACTTTGA GAAGGTCCAG 
AAGAAGCACC TGGAAGTGAG ACACCAGCGG AGTGGGCGCG GGGACCACCT GGACCGAAGG 
GTTGTCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_546837.2
CDS	305..2656
Translation	MNPEKDFAPLTPNIVRALNDKLYEKRKVAALEIEKLVREFVAQNNTVQIKHVIQTLSQEFALSQHPHSRKGGLIGLAACSIALGKDSGLYLKELIEPVLTCFNDADSRLRYYACEALYNIVKVARGAVLPHFNVLFDGLSKLAADPDPNVKSGSELLDRLLKDIVTESNKFDLVGFIPLLRERIYSNNQYARQFIISWILVLESVPDINLLDYLPEILDGLFQILGDNGKEIRKMCEVVLGEFLKEIKKNPSSVKFAEMANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILTAVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDEPDELKPVVQRQAEPNPDDSVAKQEGTATGGPDGSCDSSFSSSISVFTPASTERAPVTLHLDGIVQVLNCHLSDMAIGMMTRIAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQTDDPGPLDGPDFRVSHSELQVPTPGRANLLNTPGTKGLECSPSTPTMNSYFYKFMINLLKRFSSERKLLEARGAFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHTLNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLTEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSGAFQLLSHRLQCVPNPELLQTEDSPQATPKSQKADSPSIDYAELLRHFEKVQKKHLEVRHQRSGRGDHLDRRVVL

Target ORF information:

RefSeq Version	XM_546837.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Vac14 homolog (S. cerevisiae) (VAC14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_546837.5

ORF Insert Sequence:

ATGAACCCGG AGAAGGACTT CGCGCCGCTC ACGCCCAACA TCGTGCGCGC CCTCAATGAC 
AAGCTCTACG AAAAGCGGAA GGTGGCAGCG CTGGAGATTG AGAAGCTGGT CCGGGAGTTC 
GTGGCCCAGA ACAACACCGT GCAAATCAAG CATGTGATCC AGACCCTGTC TCAGGAGTTC 
GCCCTGTCCC AGCACCCCCA CAGCCGGAAG GGGGGCCTCA TCGGCCTGGC CGCCTGCTCT 
ATTGCACTGG GCAAGGACTC AGGGCTGTAC CTAAAGGAGC TCATTGAGCC GGTGCTGACC 
TGCTTCAATG ATGCAGACAG CAGGCTGCGC TACTACGCAT GCGAGGCACT CTACAACATC 
GTCAAGGTGG CCCGTGGCGC CGTGCTGCCT CATTTCAACG TGCTCTTCGA CGGGCTCAGC 
AAGCTGGCGG CGGACCCAGA CCCTAATGTA AAAAGTGGAT CTGAGCTCCT AGACCGCCTT 
TTAAAGGACA TTGTGACAGA GAGCAACAAG TTTGATCTGG TGGGCTTCAT CCCTTTGTTG 
CGGGAGAGGA TTTATTCCAA CAACCAGTAC GCCCGGCAGT TCATCATCTC CTGGATCCTG 
GTTCTGGAGT CCGTGCCAGA CATTAACCTG CTGGATTATT TGCCGGAGAT CCTGGATGGA 
CTCTTCCAGA TCCTGGGCGA CAACGGCAAA GAGATTCGGA AAATGTGTGA GGTAGTCCTT 
GGGGAATTCT TGAAAGAAAT CAAGAAGAAC CCCTCCAGTG TTAAGTTTGC TGAAATGGCC 
AACATCCTGG TGATCCACTG CCAGACCACA GATGACCTGA TCCAGCTGAC AGCCATGTGC 
TGGATGCGAG AGTTCATCCA GCTGGCTGGC CGAGTGATGT TGCCCTACTC ATCTGGAATC 
CTGACCGCTG TCTTGCCCTG CCTGGCCTAC GATGACCGCA AGAAAAGCAT CAAGGAGGTG 
GCCAACGTGT GTAACCAAAG CCTGATGAAG CTGGTTACCC CTGAGGATGA TGAGCCTGAC 
GAGCTGAAGC CAGTGGTACA GAGGCAGGCA GAGCCCAATC CCGATGACTC CGTGGCCAAG 
CAGGAAGGGA CAGCCACTGG AGGCCCCGAT GGTTCTTGTG ACTCAAGCTT CAGTAGCAGC 
ATCAGCGTCT TCACGCCAGC CAGCACTGAA AGGGCCCCAG TGACCCTCCA CCTCGATGGG 
ATTGTGCAGG TCCTGAACTG CCACCTCAGT GACATGGCCA TCGGGATGAT GACCAGGATT 
GCTGTCCTGA AGTGGCTCTA CCATCTGTAT ATCAAGACTC CCCGGAAGAT GTTCCGGCAC 
ACGGACAGCC TCTTCCCCAT CCTACTGCAG ACGTTATCGG ACGAATCTGA TGAGGTTATC 
CTGAAGGATC TAGAGGTATT GGCAGAAATT GCCTCCTCCC CTGCAGGCCA GACGGATGAC 
CCAGGCCCCC TTGATGGCCC TGACTTCCGG GTCAGCCACT CAGAGCTTCA GGTGCCCACC 
CCTGGCAGAG CCAACCTACT GAATACTCCT GGTACCAAAG GCTTAGAATG TTCTCCTTCC 
ACCCCCACCA TGAACTCCTA CTTTTATAAG TTCATGATCA ACCTTCTCAA GAGGTTCAGC 
AGTGAACGCA AGCTCCTGGA GGCCAGAGGC GCTTTCATCA TCAGGCAGCT CTGCCTCCTG 
CTGAATGCAG AGAACATCTT TCACTCGATG GCAGACATCC TGCTCCGAGA GGAGGACCTC 
AAGTTCGCCT CAACCATGGT CCACACGCTC AACACCATCC TGCTCACCTC CACAGAGCTC 
TTCCAGCTGA GGAACCAGCT CAAGGACCTG AAGACTCTGG AGAGCCAGAA CCTGTTCTGC 
TGCCTGTACC GCTCCTGGTG CCACAACCCA GTCACAACAG TGTCCCTGTG CTTCCTCACC 
CAGAACTACC GGCACGCCTA CGACCTCATC CAGAAGTTCG GGGACCTGGA GGTCACGGTG 
GATTTCCTCA CTGAGGTGGA CAAGCTGGTG CAGCTGATCG AGTGCCCCAT CTTCACATAC 
CTGCGCCTGC AGCTGCTCGA CGTGAAGAAC AACCCGTACC TGATCAAGGC CCTCTACGGC 
CTGCTCATGC TGCTGCCCCA GAGCGGAGCC TTCCAGCTGC TCTCCCACCG GCTGCAGTGT 
GTGCCCAACC CCGAGCTGCT ACAGACCGAA GACAGTCCGC AGGCCACCCC CAAGTCACAG 
AAAGCAGATT CCCCCAGCAT CGACTATGCA GAGCTGTTGC GGCACTTTGA GAAGGTCCAG 
AAGAAGCACC TGGAAGTGAG ACACCAGCGG AGTGGGCGCG GGGACCACCT GGACCGAAGG 
GTTGTCCTCT GA