NEURL4 cDNA ORF clone, Canis lupus familiaris(dog)

The following NEURL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEURL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe06223 XM_005619974.2
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe06224 XM_546587.5
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe06226 XM_005619976.2
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288100 XM_022418485.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288101 XM_022418486.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288102 XM_022418487.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288103 XM_022418488.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288104 XM_022418489.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288105 XM_022418490.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288106 XM_022418491.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe288107 XM_022418492.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe36406 XM_014113521.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe36407 XM_014113522.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe36408 XM_014113523.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe36409 XM_014113524.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OCe36410 XM_014113526.1
Latest version!
Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe06223
    Clone ID Related Accession (Same CDS sequence) XM_005619974.2
    Accession Version XM_005619974.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4668bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGCAGGGGAC ACTGTGGGCG TGGTGCGGCG GGAGGACGGG
    ACCCTGCACT TCTTCGTCAA CGGGATGACC CAGGGCCCTG CGGCCTGGAA TGTGCCCCCT
    GGCGTCTACG CTGTTGTGGA CCTCTATGGC CAGGCAGCCC AAGCTACCAT TGTGGATGAC
    GTGGAGGTGC CCCCGGCCCC TGAGCCCCTC TCTGAAGGGA ACAACCAGGT GTCTCCCAGC
    TCCCCATCAT CAGGGGCCGG GGGCTCTGAC CTGCGCTTCC ATCAGCTGCA CGGCAGTAAT
    GCAGTCATCA CCAACGGGGG CCGCACCGCA CTCCGGCACA ACTGCCGCAG CGAGTTCAAT
    GACGCCATCG TCATCTCCAA CCGGGCCCTG CGGGACGGAG AGCTGTTTGA AATCGTTATT
    CAGAAGATGG TGGACCGCTG GTCAGGCTCC ATTGAGGCTG GAGTGACTGC CATTCGGCCG
    GAAGACCTCG AATTCCCCAA CACCATGACA GACATTGACT ACGACACATG GATGCTGAGT
    GGCACGGCCA TCATGCAAGA CGGGAACACG ATGCGCAACA ACTACGGGTG TGACCTGGAC
    GCCCTGGGCA CGGGTGCACG CATCGGCATG ATGCGAACAG CCAAGGGTGA CCTGCACTAC
    TTCATCAACG GCCAGGACCA AGGCGCCGCC TGCTCAGGCT TGCCTCCGGG TAAAGAGGTG
    TACGCAGTAG TGGATCTTTA CGGCCAGTGT GTCCAAGTGT CCATCACCAA TGCCACCGGC
    CCCATGGACA ACAGCTTGGC AACCAGCAAC ACTGCCACCG AGAAATCCTT CCCTTTGCAC
    TCCCCAGCGG CTGGCGTGGC TCACCGATTC CACAGTACTT GCGGCAAGAA TGTGGCCCTG
    GAGGAGGATG GCACAAGGGC GGTACGTGCG GTGGGCTATG CGCATGGCCT GGTCTTCAGC
    ACCAAGGAAC TCAAGACGGA GGAAGTATTT GAGGTGAAAG TAGAGGAACT GGATGAGAAG
    TGGGCGGGCT CCCTCCGGCT GGGGCTGACC ACACTAGCGC CCGGGGAGAT GGGGCCTGGC
    GCAGGTGGGG GCCCAGGGCT GCCTCCCTCC CTCCCGGAGC TCCGGACAAA GACCACCTGG
    ATGGTGTCCA GCTGTGAAGT GAGGCGTGAC GGGCAGCTCC AGAGGATGAA CTACGGCCGG
    AGCCTGGAGA GGCTGGGGGT GGGGAGCCGC GTGGGCATTC GTCGGGGCGC AGATGACACG
    ATGCACATCC TGGTGGATGG AGAGGACATG GGGCCCGCAG CTACCGGCAT TGCCAAGAAT
    GTGTGGGCCG TGCTGGATCT CTACGGGCCG GTACGGAGTG TGTCTGTGGT CAGCTCCACA
    AGGCTGGAGG AGCCCGAGGG CACCCAGCCT CCATCTCCCA GCTCGGACAC GGGCAGCGAG
    GCCGAGGAAG ATGACGATGG GGAGGAGCAC AGCTTGAGAG GTCAGAGTCA AGTGGCCATG
    ATGTCGACGT CACTGGAGTT CCTGGAGAAC CATGGGAAGA ATATCCTTTT GTCCAACGGG
    AACCGTACGG CCACACGCGT AGCCAGCTAC AACCAGGGCA TCGTTGTCAT CAACCAGCCC
    CTGGTGCCCC AGCTCCTAGT CCAGGTGCGG ATAGACTTCC TGAACCGGCA GTGGACATCT
    TCCCTGGTCC TGGGAGTCAT CACCTGCCCG CCTGAGAGGC TCAACTTCCC TGCTTCTGCC
    TGCGCCCTCA AACGGGCAGC CTGGCTGCTG CGGGGCCGGG GCATCTTCCA CAATGGTCTC
    AAGATTTGTG AGAAGTTTGG GCCAAATCTG GACACGTGTC CTGAAGGCAC CATCCTGGGG
    CTGCGGCTAG ACAGCTCTGG GGGCCTGCAT CTCCACGTCA ATGGGATGGA CCAGGGGGTG
    GCGGTATCAG ATGTCCCCCA GCCCTGCCAT GCGCTTGTGG ACCTCTATGG GCAGTGTGAG
    CAGGTGACAA TCGTGAGTCC TGAACCAGGG GCAGCCAGTG GGAAGAGTGC TGGAACCCAA
    GGGGACATGG AGAAAGCGGA CATGGTGGAT GGTATCAAGG AAAGTGTGTG CTGGGGCCCC
    CTGCCAGCTG CCAGCCCTCT CAAGAGCTGC GAGTACCACG CCCTCTGCTC CCGCTTCCAA
    GAACTGCTGT TGCTTCCTGA GGACTATTTC ATGCCTCCAC CCAAGCGGAG CCTGTGCTAC
    TGTGAGTCTT GCCGGAAGCT TCGAGGTGAC GAGGCCCACA GGCGCCGTGG GGAGCCCCCC
    CGGGAATACG CGCTGCCCTT CGGCTGGTGC AGGTTCAATC TCAGGGTGAA TCCCCGCCTG
    GAAGCTGGAA CGATGACTAA GAAGTGGCAC ATGGCATATC ACGGGAGCAA CGTGGCAGCC
    ATCCGGAGGG TGCTAGACCG AGGAGAGCTG GGAGCAGGCA CCGCCTCCAT CCTGAGCTGC
    CGGCCCTTGA AAGGCAAGCC TGGGGTGGGG TTTGAGGAGC CTGGCGAGAA CTGCGCCCCA
    CCCCGGGAGG AGCAGCCCCC TTCCGTCTTG CTGTCCCCCT CCCTCCAGTA TGCTGGGGCC
    GAGACCCTAG CGTCCAAAGT GCAATTCCGG GATCCCAAGT CCCAGCGAAC GCACCAGGCC
    CAGGTGGCGT TCCAGGTGTG CGTGCGCCCG GGCTCCTACA CCCCCGGACC CCCTTCGGCC
    ACCCTCCGAG AACCTCCCGA CCCTCACTTC AGCCCCGCAG AACTCGAGTG GATAACCAAG
    GAGAAGGGGG CCACACTCCT CTATGCCCTG CTGGTGCGGG TGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620031.1
    CDS1..4668
    Translation

    Target ORF information:

    RefSeq Version XM_005619974.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619974.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGCAGGGGAC ACTGTGGGCG TGGTGCGGCG GGAGGACGGG
    ACCCTGCACT TCTTCGTCAA CGGGATGACC CAGGGCCCTG CGGCCTGGAA TGTGCCCCCT
    GGCGTCTACG CTGTTGTGGA CCTCTATGGC CAGGCAGCCC AAGCTACCAT TGTGGATGAC
    GTGGAGGTGC CCCCGGCCCC TGAGCCCCTC TCTGAAGGGA ACAACCAGGT GTCTCCCAGC
    TCCCCATCAT CAGGGGCCGG GGGCTCTGAC CTGCGCTTCC ATCAGCTGCA CGGCAGTAAT
    GCAGTCATCA CCAACGGGGG CCGCACCGCA CTCCGGCACA ACTGCCGCAG CGAGTTCAAT
    GACGCCATCG TCATCTCCAA CCGGGCCCTG CGGGACGGAG AGCTGTTTGA AATCGTTATT
    CAGAAGATGG TGGACCGCTG GTCAGGCTCC ATTGAGGCTG GAGTGACTGC CATTCGGCCG
    GAAGACCTCG AATTCCCCAA CACCATGACA GACATTGACT ACGACACATG GATGCTGAGT
    GGCACGGCCA TCATGCAAGA CGGGAACACG ATGCGCAACA ACTACGGGTG TGACCTGGAC
    GCCCTGGGCA CGGGTGCACG CATCGGCATG ATGCGAACAG CCAAGGGTGA CCTGCACTAC
    TTCATCAACG GCCAGGACCA AGGCGCCGCC TGCTCAGGCT TGCCTCCGGG TAAAGAGGTG
    TACGCAGTAG TGGATCTTTA CGGCCAGTGT GTCCAAGTGT CCATCACCAA TGCCACCGGC
    CCCATGGACA ACAGCTTGGC AACCAGCAAC ACTGCCACCG AGAAATCCTT CCCTTTGCAC
    TCCCCAGCGG CTGGCGTGGC TCACCGATTC CACAGTACTT GCGGCAAGAA TGTGGCCCTG
    GAGGAGGATG GCACAAGGGC GGTACGTGCG GTGGGCTATG CGCATGGCCT GGTCTTCAGC
    ACCAAGGAAC TCAAGACGGA GGAAGTATTT GAGGTGAAAG TAGAGGAACT GGATGAGAAG
    TGGGCGGGCT CCCTCCGGCT GGGGCTGACC ACACTAGCGC CCGGGGAGAT GGGGCCTGGC
    GCAGGTGGGG GCCCAGGGCT GCCTCCCTCC CTCCCGGAGC TCCGGACAAA GACCACCTGG
    ATGGTGTCCA GCTGTGAAGT GAGGCGTGAC GGGCAGCTCC AGAGGATGAA CTACGGCCGG
    AGCCTGGAGA GGCTGGGGGT GGGGAGCCGC GTGGGCATTC GTCGGGGCGC AGATGACACG
    ATGCACATCC TGGTGGATGG AGAGGACATG GGGCCCGCAG CTACCGGCAT TGCCAAGAAT
    GTGTGGGCCG TGCTGGATCT CTACGGGCCG GTACGGAGTG TGTCTGTGGT CAGCTCCACA
    AGGCTGGAGG AGCCCGAGGG CACCCAGCCT CCATCTCCCA GCTCGGACAC GGGCAGCGAG
    GCCGAGGAAG ATGACGATGG GGAGGAGCAC AGCTTGAGAG GTCAGAGTCA AGTGGCCATG
    ATGTCGACGT CACTGGAGTT CCTGGAGAAC CATGGGAAGA ATATCCTTTT GTCCAACGGG
    AACCGTACGG CCACACGCGT AGCCAGCTAC AACCAGGGCA TCGTTGTCAT CAACCAGCCC
    CTGGTGCCCC AGCTCCTAGT CCAGGTGCGG ATAGACTTCC TGAACCGGCA GTGGACATCT
    TCCCTGGTCC TGGGAGTCAT CACCTGCCCG CCTGAGAGGC TCAACTTCCC TGCTTCTGCC
    TGCGCCCTCA AACGGGCAGC CTGGCTGCTG CGGGGCCGGG GCATCTTCCA CAATGGTCTC
    AAGATTTGTG AGAAGTTTGG GCCAAATCTG GACACGTGTC CTGAAGGCAC CATCCTGGGG
    CTGCGGCTAG ACAGCTCTGG GGGCCTGCAT CTCCACGTCA ATGGGATGGA CCAGGGGGTG
    GCGGTATCAG ATGTCCCCCA GCCCTGCCAT GCGCTTGTGG ACCTCTATGG GCAGTGTGAG
    CAGGTGACAA TCGTGAGTCC TGAACCAGGG GCAGCCAGTG GGAAGAGTGC TGGAACCCAA
    GGGGACATGG AGAAAGCGGA CATGGTGGAT GGTATCAAGG AAAGTGTGTG CTGGGGCCCC
    CTGCCAGCTG CCAGCCCTCT CAAGAGCTGC GAGTACCACG CCCTCTGCTC CCGCTTCCAA
    GAACTGCTGT TGCTTCCTGA GGACTATTTC ATGCCTCCAC CCAAGCGGAG CCTGTGCTAC
    TGTGAGTCTT GCCGGAAGCT TCGAGGTGAC GAGGCCCACA GGCGCCGTGG GGAGCCCCCC
    CGGGAATACG CGCTGCCCTT CGGCTGGTGC AGGTTCAATC TCAGGGTGAA TCCCCGCCTG
    GAAGCTGGAA CGATGACTAA GAAGTGGCAC ATGGCATATC ACGGGAGCAA CGTGGCAGCC
    ATCCGGAGGG TGCTAGACCG AGGAGAGCTG GGAGCAGGCA CCGCCTCCAT CCTGAGCTGC
    CGGCCCTTGA AAGGCAAGCC TGGGGTGGGG TTTGAGGAGC CTGGCGAGAA CTGCGCCCCA
    CCCCGGGAGG AGCAGCCCCC TTCCGTCTTG CTGTCCCCCT CCCTCCAGTA TGCTGGGGCC
    GAGACCCTAG CGTCCAAAGT GCAATTCCGG GATCCCAAGT CCCAGCGAAC GCACCAGGCC
    CAGGTGGCGT TCCAGGTGTG CGTGCGCCCG GGCTCCTACA CCCCCGGACC CCCTTCGGCC
    ACCCTCCGAG AACCTCCCGA CCCTCACTTC AGCCCCGCAG AACTCGAGTG GATAACCAAG
    GAGAAGGGGG CCACACTCCT CTATGCCCTG CTGGTGCGGG TGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06224
    Clone ID Related Accession (Same CDS sequence) XM_546587.5
    Accession Version XM_546587.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546587.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGCAGGGGAC ACTGTGGGCG TGGTGCGGCG GGAGGACGGG
    ACCCTGCACT TCTTCGTCAA CGGGATGACC CAGGGCCCTG CGGCCTGGAA TGTGCCCCCT
    GGCGTCTACG CTGTTGTGGA CCTCTATGGC CAGGCAGCCC AAGCTACCAT TGTGGATGAC
    GTGGAGGTGC CCCCGGCCCC TGAGCCCCTC TCTGAAGGGA ACAACCAGGT GTCTCCCAGC
    TCCCCATCAT CAGGGGCCGG GGGCTCTGAC CTGCGCTTCC ATCAGCTGCA CGGCAGTAAT
    GCAGTCATCA CCAACGGGGG CCGCACCGCA CTCCGGCACA ACTGCCGCAG CGAGTTCAAT
    GACGCCATCG TCATCTCCAA CCGGGCCCTG CGGGACGGAG AGCTGTTTGA AATCGTTATT
    CAGAAGATGG TGGACCGCTG GTCAGGCTCC ATTGAGGCTG GAGTGACTGC CATTCGGCCG
    GAAGACCTCG AATTCCCCAA CACCATGACA GACATTGACT ACGACACATG GATGCTGAGT
    GGCACGGCCA TCATGCAAGA CGGGAACACG ATGCGCAACA ACTACGGGTG TGACCTGGAC
    GCCCTGGGCA CGGGTGCACG CATCGGCATG ATGCGAACAG CCAAGGGTGA CCTGCACTAC
    TTCATCAACG GCCAGGACCA AGGCGCCGCC TGCTCAGGCT TGCCTCCGGA GGTGTACGCA
    GTAGTGGATC TTTACGGCCA GTGTGTCCAA GTGTCCATCA CCAATGCCAC CGGCCCCATG
    GACAACAGCT TGGCAACCAG CAACACTGCC ACCGAGAAAT CCTTCCCTTT GCACTCCCCA
    GCGGCTGGCG TGGCTCACCG ATTCCACAGT ACTTGCGGCA AGAATGTGGC CCTGGAGGAG
    GATGGCACAA GGGCGGTACG TGCGGTGGGC TATGCGCATG GCCTGGTCTT CAGCACCAAG
    GAACTCAAGA CGGAGGAAGT ATTTGAGGTG AAAGTAGAGG AACTGGATGA GAAGTGGGCG
    GGCTCCCTCC GGCTGGGGCT GACCACACTA GCGCCCGGGG AGATGGGGCC TGGCGCAGGT
    GGGGGCCCAG GGCTGCCTCC CTCCCTCCCG GAGCTCCGGA CAAAGACCAC CTGGATGGTG
    TCCAGCTGTG AAGTGAGGCG TGACGGGCAG CTCCAGAGGA TGAACTACGG CCGGAGCCTG
    GAGAGGCTGG GGGTGGGGAG CCGCGTGGGC ATTCGTCGGG GCGCAGATGA CACGATGCAC
    ATCCTGGTGG ATGGAGAGGA CATGGGGCCC GCAGCTACCG GCATTGCCAA GAATGTGTGG
    GCCGTGCTGG ATCTCTACGG GCCGGTACGG AGTGTGTCTG TGGTCAGCTC CACAAGGCTG
    GAGGAGCCCG AGGGCACCCA GCCTCCATCT CCCAGCTCGG ACACGGGCAG CGAGGCCGAG
    GAAGATGACG ATGGGGAGGA GCACAGCTTG AGAGGTCAGA GTCAAGTGGC CATGATGTCG
    ACGTCACTGG AGTTCCTGGA GAACCATGGG AAGAATATCC TTTTGTCCAA CGGGAACCGT
    ACGGCCACAC GCGTAGCCAG CTACAACCAG GGCATCGTTG TCATCAACCA GCCCCTGGTG
    CCCCAGCTCC TAGTCCAGGT GCGGATAGAC TTCCTGAACC GGCAGTGGAC ATCTTCCCTG
    GTCCTGGGAG TCATCACCTG CCCGCCTGAG AGGCTCAACT TCCCTGCTTC TGCCTGCGCC
    CTCAAACGGG CAGCCTGGCT GCTGCGGGGC CGGGGCATCT TCCACAATGG TCTCAAGATT
    TGTGAGAAGT TTGGGCCAAA TCTGGACACG TGTCCTGAAG GCACCATCCT GGGGCTGCGG
    CTAGACAGCT CTGGGGGCCT GCATCTCCAC GTCAATGGGA TGGACCAGGG GGTGGCGGTA
    TCAGATGTCC CCCAGCCCTG CCATGCGCTT GTGGACCTCT ATGGGCAGTG TGAGCAGGTG
    ACAATCGTGA GTCCTGAACC AGGGGCAGCC AGTGGGAAGA GTGCTGGAAC CCAAGGGGAC
    ATGGAGAAAG CGGACATGGT GGATGGTATC AAGGAAAGTG TGTGCTGGGG CCCCCTGCCA
    GCTGCCAGCC CTCTCAAGAG CTGCGAGTAC CACGCCCTCT GCTCCCGCTT CCAAGAACTG
    CTGTTGCTTC CTGAGGACTA TTTCATGCCT CCACCCAAGC GGAGCCTGTG CTACTGTGAG
    TCTTGCCGGA AGCTTCGAGG TGACGAGGCC CACAGGCGCC GTGGGGAGCC CCCCCGGGAA
    TACGCGCTGC CCTTCGGCTG GTGCAGGTTC AATCTCAGGG TGAATCCCCG CCTGGAAGCT
    GGAACGATGA CTAAGAAGTG GCACATGGCA TATCACGGGA GCAACGTGGC AGCCATCCGG
    AGGGTGCTAG ACCGAGGAGA GCTGGGAGCA GGCACCGCCT CCATCCTGAG CTGCCGGCCC
    TTGAAAGGCA AGCCTGGGGT GGGGTTTGAG GAGCCTGGCG AGAACTGCGC CCCACCCCGG
    GAGGAGCAGC CCCCTTCCGT CTTGCTGTCC CCCTCCCTCC AGTATGCTGG GGCCGAGACC
    CTAGCGTCCA AAGTGCAATT CCGGGATCCC AAGTCCCAGC GAACGCACCA GGCCCAGGTG
    GCGTTCCAGG TGTGCGTGCG CCCGGGCTCC TACACCCCCG GACCCCCTTC GGCCACCCTC
    CGAGAACCTC CCGACCCTCA CTTCAGCCCC GCAGAACTCG AGTGGATAAC CAAGGAGAAG
    GGGGCCACAC TCCTCTATGC CCTGCTGGTG CGGGTGGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546587.2
    CDS1..4662
    Translation

    Target ORF information:

    RefSeq Version XM_546587.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546587.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGCAGGGGAC ACTGTGGGCG TGGTGCGGCG GGAGGACGGG
    ACCCTGCACT TCTTCGTCAA CGGGATGACC CAGGGCCCTG CGGCCTGGAA TGTGCCCCCT
    GGCGTCTACG CTGTTGTGGA CCTCTATGGC CAGGCAGCCC AAGCTACCAT TGTGGATGAC
    GTGGAGGTGC CCCCGGCCCC TGAGCCCCTC TCTGAAGGGA ACAACCAGGT GTCTCCCAGC
    TCCCCATCAT CAGGGGCCGG GGGCTCTGAC CTGCGCTTCC ATCAGCTGCA CGGCAGTAAT
    GCAGTCATCA CCAACGGGGG CCGCACCGCA CTCCGGCACA ACTGCCGCAG CGAGTTCAAT
    GACGCCATCG TCATCTCCAA CCGGGCCCTG CGGGACGGAG AGCTGTTTGA AATCGTTATT
    CAGAAGATGG TGGACCGCTG GTCAGGCTCC ATTGAGGCTG GAGTGACTGC CATTCGGCCG
    GAAGACCTCG AATTCCCCAA CACCATGACA GACATTGACT ACGACACATG GATGCTGAGT
    GGCACGGCCA TCATGCAAGA CGGGAACACG ATGCGCAACA ACTACGGGTG TGACCTGGAC
    GCCCTGGGCA CGGGTGCACG CATCGGCATG ATGCGAACAG CCAAGGGTGA CCTGCACTAC
    TTCATCAACG GCCAGGACCA AGGCGCCGCC TGCTCAGGCT TGCCTCCGGA GGTGTACGCA
    GTAGTGGATC TTTACGGCCA GTGTGTCCAA GTGTCCATCA CCAATGCCAC CGGCCCCATG
    GACAACAGCT TGGCAACCAG CAACACTGCC ACCGAGAAAT CCTTCCCTTT GCACTCCCCA
    GCGGCTGGCG TGGCTCACCG ATTCCACAGT ACTTGCGGCA AGAATGTGGC CCTGGAGGAG
    GATGGCACAA GGGCGGTACG TGCGGTGGGC TATGCGCATG GCCTGGTCTT CAGCACCAAG
    GAACTCAAGA CGGAGGAAGT ATTTGAGGTG AAAGTAGAGG AACTGGATGA GAAGTGGGCG
    GGCTCCCTCC GGCTGGGGCT GACCACACTA GCGCCCGGGG AGATGGGGCC TGGCGCAGGT
    GGGGGCCCAG GGCTGCCTCC CTCCCTCCCG GAGCTCCGGA CAAAGACCAC CTGGATGGTG
    TCCAGCTGTG AAGTGAGGCG TGACGGGCAG CTCCAGAGGA TGAACTACGG CCGGAGCCTG
    GAGAGGCTGG GGGTGGGGAG CCGCGTGGGC ATTCGTCGGG GCGCAGATGA CACGATGCAC
    ATCCTGGTGG ATGGAGAGGA CATGGGGCCC GCAGCTACCG GCATTGCCAA GAATGTGTGG
    GCCGTGCTGG ATCTCTACGG GCCGGTACGG AGTGTGTCTG TGGTCAGCTC CACAAGGCTG
    GAGGAGCCCG AGGGCACCCA GCCTCCATCT CCCAGCTCGG ACACGGGCAG CGAGGCCGAG
    GAAGATGACG ATGGGGAGGA GCACAGCTTG AGAGGTCAGA GTCAAGTGGC CATGATGTCG
    ACGTCACTGG AGTTCCTGGA GAACCATGGG AAGAATATCC TTTTGTCCAA CGGGAACCGT
    ACGGCCACAC GCGTAGCCAG CTACAACCAG GGCATCGTTG TCATCAACCA GCCCCTGGTG
    CCCCAGCTCC TAGTCCAGGT GCGGATAGAC TTCCTGAACC GGCAGTGGAC ATCTTCCCTG
    GTCCTGGGAG TCATCACCTG CCCGCCTGAG AGGCTCAACT TCCCTGCTTC TGCCTGCGCC
    CTCAAACGGG CAGCCTGGCT GCTGCGGGGC CGGGGCATCT TCCACAATGG TCTCAAGATT
    TGTGAGAAGT TTGGGCCAAA TCTGGACACG TGTCCTGAAG GCACCATCCT GGGGCTGCGG
    CTAGACAGCT CTGGGGGCCT GCATCTCCAC GTCAATGGGA TGGACCAGGG GGTGGCGGTA
    TCAGATGTCC CCCAGCCCTG CCATGCGCTT GTGGACCTCT ATGGGCAGTG TGAGCAGGTG
    ACAATCGTGA GTCCTGAACC AGGGGCAGCC AGTGGGAAGA GTGCTGGAAC CCAAGGGGAC
    ATGGAGAAAG CGGACATGGT GGATGGTATC AAGGAAAGTG TGTGCTGGGG CCCCCTGCCA
    GCTGCCAGCC CTCTCAAGAG CTGCGAGTAC CACGCCCTCT GCTCCCGCTT CCAAGAACTG
    CTGTTGCTTC CTGAGGACTA TTTCATGCCT CCACCCAAGC GGAGCCTGTG CTACTGTGAG
    TCTTGCCGGA AGCTTCGAGG TGACGAGGCC CACAGGCGCC GTGGGGAGCC CCCCCGGGAA
    TACGCGCTGC CCTTCGGCTG GTGCAGGTTC AATCTCAGGG TGAATCCCCG CCTGGAAGCT
    GGAACGATGA CTAAGAAGTG GCACATGGCA TATCACGGGA GCAACGTGGC AGCCATCCGG
    AGGGTGCTAG ACCGAGGAGA GCTGGGAGCA GGCACCGCCT CCATCCTGAG CTGCCGGCCC
    TTGAAAGGCA AGCCTGGGGT GGGGTTTGAG GAGCCTGGCG AGAACTGCGC CCCACCCCGG
    GAGGAGCAGC CCCCTTCCGT CTTGCTGTCC CCCTCCCTCC AGTATGCTGG GGCCGAGACC
    CTAGCGTCCA AAGTGCAATT CCGGGATCCC AAGTCCCAGC GAACGCACCA GGCCCAGGTG
    GCGTTCCAGG TGTGCGTGCG CCCGGGCTCC TACACCCCCG GACCCCCTTC GGCCACCCTC
    CGAGAACCTC CCGACCCTCA CTTCAGCCCC GCAGAACTCG AGTGGATAAC CAAGGAGAAG
    GGGGCCACAC TCCTCTATGC CCTGCTGGTG CGGGTGGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06226
    Clone ID Related Accession (Same CDS sequence) XM_005619976.2
    Accession Version XM_005619976.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4596bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619976.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGA CTTTGCCAGC
    ATGGAGCTCT CTGAGGTGGT GGGCAACGCC ATCCTTTCTG CCTACAACGG GGGGCTCCTA
    AATGTGAACC TGAGCTCCCC ACCGGCAGGG GAAGGACTGG GGTCTGGCGG GGCTGCCACC
    TCGCCCATCC TCACCTCCAA CGACGCCCTG CTCTTTCACG AGAAGTGTGG GACTCTCATC
    AAACTCAGCA ACAACAATAA GACAGCTGAG CGCCGCCGGC CCCTGGACGA ATTCAACAAC
    GGGGTTGTCA TGACCAACCG CCCTCTCCGG GACAATGAGA TGTTTGAGAT CCGCATTGAC
    AAGCTCGTAG ACAAGTGGTC GGGCTCCATT GAGATTGGTG TCACCACCCA CAACCCCAAC
    AACCTGGAGT ACCCTGCCAC CATGACCAAT TTACAGTCAG GCACCATCAT GATGAGCGGC
    TGTGGGATCC TGACCAATGG CAAGGGCACC CGCCGGGAGT ACTGTGAATT CAGCCTGGAT
    GAGCTGCAGG AGGGCGACCA CATTGGTCTC ACAAGGAAGT CCAACTCAGC CCTGCACTTC
    TTTATCAATG GCATTGATCA GGGCGTGGCT ACCCCACTGA CACCCCCAGT GGTGTACGGC
    GTGGTGGACT TGTACGGGAT GGCTGTGAAG GTGACCATCG TCCACAATAA CAACCACAGC
    GACCGGCTGC GTCGAAACAA TGCCATCCTG CGGGCACTGT CCCCCGAGGG CGCCCTGCGT
    CGCGCCACTC CTGCCACCCA GGCAGAGCCC GAGCGCCTGC TCTTCCACCC TAACTGCGGG
    CAGAAGGCAG CTATCACCCA CGAGGGACGC ACTGCCCTGA GGCCCCATGC CACAGATGAC
    TTCAACCATG GCGTGGTGCT GAGTAGCAGA GCCCTGCGGG ACGGAGAGGT GTTTCAGGTG
    CGCATCGACA AGATGGTGGA CAAGTGGGCT GGCTCCATTG AGATTGGTGT CACCACCCAC
    AACCCTGCCT ACCTCCAGTT GCCCTCCACC ATGACCAACT TGCGCTCTGG GACCTGGATG
    ATGACCGGGA ATGGGGTGAT GCACAACGGA ACGACCATTT TGGACGAGTA TGGGCACAAT
    CTGGACCGCC TCAAGGCAGG GGACACTGTG GGCGTGGTGC GGCGGGAGGA CGGGACCCTG
    CACTTCTTCG TCAACGGGAT GACCCAGGGC CCTGCGGCCT GGAATGTGCC CCCTGGCGTC
    TACGCTGTTG TGGACCTCTA TGGCCAGGCA GCCCAAGCTA CCATTGTGGA TGACGTGGAG
    GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA GGGAACAACC AGGTGTCTCC CAGCTCCCCA
    TCATCAGGGG CCGGGGGCTC TGACCTGCGC TTCCATCAGC TGCACGGCAG TAATGCAGTC
    ATCACCAACG GGGGCCGCAC CGCACTCCGG CACAACTGCC GCAGCGAGTT CAATGACGCC
    ATCGTCATCT CCAACCGGGC CCTGCGGGAC GGAGAGCTGT TTGAAATCGT TATTCAGAAG
    ATGGTGGACC GCTGGTCAGG CTCCATTGAG GCTGGAGTGA CTGCCATTCG GCCGGAAGAC
    CTCGAATTCC CCAACACCAT GACAGACATT GACTACGACA CATGGATGCT GAGTGGCACG
    GCCATCATGC AAGACGGGAA CACGATGCGC AACAACTACG GGTGTGACCT GGACGCCCTG
    GGCACGGGTG CACGCATCGG CATGATGCGA ACAGCCAAGG GTGACCTGCA CTACTTCATC
    AACGGCCAGG ACCAAGGCGC CGCCTGCTCA GGCTTGCCTC CGGAGGTGTA CGCAGTAGTG
    GATCTTTACG GCCAGTGTGT CCAAGTGTCC ATCACCAATG CCACCGGCCC CATGGACAAC
    AGCTTGGCAA CCAGCAACAC TGCCACCGAG AAATCCTTCC CTTTGCACTC CCCAGCGGCT
    GGCGTGGCTC ACCGATTCCA CAGTACTTGC GGCAAGAATG TGGCCCTGGA GGAGGATGGC
    ACAAGGGCGG TACGTGCGGT GGGCTATGCG CATGGCCTGG TCTTCAGCAC CAAGGAACTC
    AAGACGGAGG AAGTATTTGA GGTGAAAGTA GAGGAACTGG ATGAGAAGTG GGCGGGCTCC
    CTCCGGCTGG GGCTGACCAC ACTAGCGCCC GGGGAGATGG GGCCTGGCGC AGGTGGGGGC
    CCAGGGCTGC CTCCCTCCCT CCCGGAGCTC CGGACAAAGA CCACCTGGAT GGTGTCCAGC
    TGTGAAGTGA GGCGTGACGG GCAGCTCCAG AGGATGAACT ACGGCCGGAG CCTGGAGAGG
    CTGGGGGTGG GGAGCCGCGT GGGCATTCGT CGGGGCGCAG ATGACACGAT GCACATCCTG
    GTGGATGGAG AGGACATGGG GCCCGCAGCT ACCGGCATTG CCAAGAATGT GTGGGCCGTG
    CTGGATCTCT ACGGGCCGGT ACGGAGTGTG TCTGTGGTCA GCTCCACAAG GCTGGAGGAG
    CCCGAGGGCA CCCAGCCTCC ATCTCCCAGC TCGGACACGG GCAGCGAGGC CGAGGAAGAT
    GACGATGGGG AGGAGCACAG CTTGAGAGGT CAGAGTCAAG TGGCCATGAT GTCGACGTCA
    CTGGAGTTCC TGGAGAACCA TGGGAAGAAT ATCCTTTTGT CCAACGGGAA CCGTACGGCC
    ACACGCGTAG CCAGCTACAA CCAGGGCATC GTTGTCATCA ACCAGCCCCT GGTGCCCCAG
    CTCCTAGTCC AGGTGCGGAT AGACTTCCTG AACCGGCAGT GGACATCTTC CCTGGTCCTG
    GGAGTCATCA CCTGCCCGCC TGAGAGGCTC AACTTCCCTG CTTCTGCCTG CGCCCTCAAA
    CGGGCAGCCT GGCTGCTGCG GGGCCGGGGC ATCTTCCACA ATGGTCTCAA GATTTGTGAG
    AAGTTTGGGC CAAATCTGGA CACGTGTCCT GAAGGCACCA TCCTGGGGCT GCGGCTAGAC
    AGCTCTGGGG GCCTGCATCT CCACGTCAAT GGGATGGACC AGGGGGTGGC GGTATCAGAT
    GTCCCCCAGC CCTGCCATGC GCTTGTGGAC CTCTATGGGC AGTGTGAGCA GGTGACAATC
    GTGAGTCCTG AACCAGGGGC AGCCAGTGGG AAGAGTGCTG GAACCCAAGG GGACATGGAG
    AAAGCGGACA TGGTGGATGG TATCAAGGAA AGTGTGTGCT GGGGCCCCCT GCCAGCTGCC
    AGCCCTCTCA AGAGCTGCGA GTACCACGCC CTCTGCTCCC GCTTCCAAGA ACTGCTGTTG
    CTTCCTGAGG ACTATTTCAT GCCTCCACCC AAGCGGAGCC TGTGCTACTG TGAGTCTTGC
    CGGAAGCTTC GAGGTGACGA GGCCCACAGG CGCCGTGGGG AGCCCCCCCG GGAATACGCG
    CTGCCCTTCG GCTGGTGCAG GTTCAATCTC AGGGTGAATC CCCGCCTGGA AGCTGGAACG
    ATGACTAAGA AGTGGCACAT GGCATATCAC GGGAGCAACG TGGCAGCCAT CCGGAGGGTG
    CTAGACCGAG GAGAGCTGGG AGCAGGCACC GCCTCCATCC TGAGCTGCCG GCCCTTGAAA
    GGCAAGCCTG GGGTGGGGTT TGAGGAGCCT GGCGAGAACT GCGCCCCACC CCGGGAGGAG
    CAGCCCCCTT CCGTCTTGCT GTCCCCCTCC CTCCAGTATG CTGGGGCCGA GACCCTAGCG
    TCCAAAGTGC AATTCCGGGA TCCCAAGTCC CAGCGAACGC ACCAGGCCCA GGTGGCGTTC
    CAGGTGTGCG TGCGCCCGGG CTCCTACACC CCCGGACCCC CTTCGGCCAC CCTCCGAGAA
    CCTCCCGACC CTCACTTCAG CCCCGCAGAA CTCGAGTGGA TAACCAAGGA GAAGGGGGCC
    ACACTCCTCT ATGCCCTGCT GGTGCGGGTG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620033.1
    CDS1..4596
    Translation

    Target ORF information:

    RefSeq Version XM_005619976.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619976.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGA CTTTGCCAGC
    ATGGAGCTCT CTGAGGTGGT GGGCAACGCC ATCCTTTCTG CCTACAACGG GGGGCTCCTA
    AATGTGAACC TGAGCTCCCC ACCGGCAGGG GAAGGACTGG GGTCTGGCGG GGCTGCCACC
    TCGCCCATCC TCACCTCCAA CGACGCCCTG CTCTTTCACG AGAAGTGTGG GACTCTCATC
    AAACTCAGCA ACAACAATAA GACAGCTGAG CGCCGCCGGC CCCTGGACGA ATTCAACAAC
    GGGGTTGTCA TGACCAACCG CCCTCTCCGG GACAATGAGA TGTTTGAGAT CCGCATTGAC
    AAGCTCGTAG ACAAGTGGTC GGGCTCCATT GAGATTGGTG TCACCACCCA CAACCCCAAC
    AACCTGGAGT ACCCTGCCAC CATGACCAAT TTACAGTCAG GCACCATCAT GATGAGCGGC
    TGTGGGATCC TGACCAATGG CAAGGGCACC CGCCGGGAGT ACTGTGAATT CAGCCTGGAT
    GAGCTGCAGG AGGGCGACCA CATTGGTCTC ACAAGGAAGT CCAACTCAGC CCTGCACTTC
    TTTATCAATG GCATTGATCA GGGCGTGGCT ACCCCACTGA CACCCCCAGT GGTGTACGGC
    GTGGTGGACT TGTACGGGAT GGCTGTGAAG GTGACCATCG TCCACAATAA CAACCACAGC
    GACCGGCTGC GTCGAAACAA TGCCATCCTG CGGGCACTGT CCCCCGAGGG CGCCCTGCGT
    CGCGCCACTC CTGCCACCCA GGCAGAGCCC GAGCGCCTGC TCTTCCACCC TAACTGCGGG
    CAGAAGGCAG CTATCACCCA CGAGGGACGC ACTGCCCTGA GGCCCCATGC CACAGATGAC
    TTCAACCATG GCGTGGTGCT GAGTAGCAGA GCCCTGCGGG ACGGAGAGGT GTTTCAGGTG
    CGCATCGACA AGATGGTGGA CAAGTGGGCT GGCTCCATTG AGATTGGTGT CACCACCCAC
    AACCCTGCCT ACCTCCAGTT GCCCTCCACC ATGACCAACT TGCGCTCTGG GACCTGGATG
    ATGACCGGGA ATGGGGTGAT GCACAACGGA ACGACCATTT TGGACGAGTA TGGGCACAAT
    CTGGACCGCC TCAAGGCAGG GGACACTGTG GGCGTGGTGC GGCGGGAGGA CGGGACCCTG
    CACTTCTTCG TCAACGGGAT GACCCAGGGC CCTGCGGCCT GGAATGTGCC CCCTGGCGTC
    TACGCTGTTG TGGACCTCTA TGGCCAGGCA GCCCAAGCTA CCATTGTGGA TGACGTGGAG
    GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA GGGAACAACC AGGTGTCTCC CAGCTCCCCA
    TCATCAGGGG CCGGGGGCTC TGACCTGCGC TTCCATCAGC TGCACGGCAG TAATGCAGTC
    ATCACCAACG GGGGCCGCAC CGCACTCCGG CACAACTGCC GCAGCGAGTT CAATGACGCC
    ATCGTCATCT CCAACCGGGC CCTGCGGGAC GGAGAGCTGT TTGAAATCGT TATTCAGAAG
    ATGGTGGACC GCTGGTCAGG CTCCATTGAG GCTGGAGTGA CTGCCATTCG GCCGGAAGAC
    CTCGAATTCC CCAACACCAT GACAGACATT GACTACGACA CATGGATGCT GAGTGGCACG
    GCCATCATGC AAGACGGGAA CACGATGCGC AACAACTACG GGTGTGACCT GGACGCCCTG
    GGCACGGGTG CACGCATCGG CATGATGCGA ACAGCCAAGG GTGACCTGCA CTACTTCATC
    AACGGCCAGG ACCAAGGCGC CGCCTGCTCA GGCTTGCCTC CGGAGGTGTA CGCAGTAGTG
    GATCTTTACG GCCAGTGTGT CCAAGTGTCC ATCACCAATG CCACCGGCCC CATGGACAAC
    AGCTTGGCAA CCAGCAACAC TGCCACCGAG AAATCCTTCC CTTTGCACTC CCCAGCGGCT
    GGCGTGGCTC ACCGATTCCA CAGTACTTGC GGCAAGAATG TGGCCCTGGA GGAGGATGGC
    ACAAGGGCGG TACGTGCGGT GGGCTATGCG CATGGCCTGG TCTTCAGCAC CAAGGAACTC
    AAGACGGAGG AAGTATTTGA GGTGAAAGTA GAGGAACTGG ATGAGAAGTG GGCGGGCTCC
    CTCCGGCTGG GGCTGACCAC ACTAGCGCCC GGGGAGATGG GGCCTGGCGC AGGTGGGGGC
    CCAGGGCTGC CTCCCTCCCT CCCGGAGCTC CGGACAAAGA CCACCTGGAT GGTGTCCAGC
    TGTGAAGTGA GGCGTGACGG GCAGCTCCAG AGGATGAACT ACGGCCGGAG CCTGGAGAGG
    CTGGGGGTGG GGAGCCGCGT GGGCATTCGT CGGGGCGCAG ATGACACGAT GCACATCCTG
    GTGGATGGAG AGGACATGGG GCCCGCAGCT ACCGGCATTG CCAAGAATGT GTGGGCCGTG
    CTGGATCTCT ACGGGCCGGT ACGGAGTGTG TCTGTGGTCA GCTCCACAAG GCTGGAGGAG
    CCCGAGGGCA CCCAGCCTCC ATCTCCCAGC TCGGACACGG GCAGCGAGGC CGAGGAAGAT
    GACGATGGGG AGGAGCACAG CTTGAGAGGT CAGAGTCAAG TGGCCATGAT GTCGACGTCA
    CTGGAGTTCC TGGAGAACCA TGGGAAGAAT ATCCTTTTGT CCAACGGGAA CCGTACGGCC
    ACACGCGTAG CCAGCTACAA CCAGGGCATC GTTGTCATCA ACCAGCCCCT GGTGCCCCAG
    CTCCTAGTCC AGGTGCGGAT AGACTTCCTG AACCGGCAGT GGACATCTTC CCTGGTCCTG
    GGAGTCATCA CCTGCCCGCC TGAGAGGCTC AACTTCCCTG CTTCTGCCTG CGCCCTCAAA
    CGGGCAGCCT GGCTGCTGCG GGGCCGGGGC ATCTTCCACA ATGGTCTCAA GATTTGTGAG
    AAGTTTGGGC CAAATCTGGA CACGTGTCCT GAAGGCACCA TCCTGGGGCT GCGGCTAGAC
    AGCTCTGGGG GCCTGCATCT CCACGTCAAT GGGATGGACC AGGGGGTGGC GGTATCAGAT
    GTCCCCCAGC CCTGCCATGC GCTTGTGGAC CTCTATGGGC AGTGTGAGCA GGTGACAATC
    GTGAGTCCTG AACCAGGGGC AGCCAGTGGG AAGAGTGCTG GAACCCAAGG GGACATGGAG
    AAAGCGGACA TGGTGGATGG TATCAAGGAA AGTGTGTGCT GGGGCCCCCT GCCAGCTGCC
    AGCCCTCTCA AGAGCTGCGA GTACCACGCC CTCTGCTCCC GCTTCCAAGA ACTGCTGTTG
    CTTCCTGAGG ACTATTTCAT GCCTCCACCC AAGCGGAGCC TGTGCTACTG TGAGTCTTGC
    CGGAAGCTTC GAGGTGACGA GGCCCACAGG CGCCGTGGGG AGCCCCCCCG GGAATACGCG
    CTGCCCTTCG GCTGGTGCAG GTTCAATCTC AGGGTGAATC CCCGCCTGGA AGCTGGAACG
    ATGACTAAGA AGTGGCACAT GGCATATCAC GGGAGCAACG TGGCAGCCAT CCGGAGGGTG
    CTAGACCGAG GAGAGCTGGG AGCAGGCACC GCCTCCATCC TGAGCTGCCG GCCCTTGAAA
    GGCAAGCCTG GGGTGGGGTT TGAGGAGCCT GGCGAGAACT GCGCCCCACC CCGGGAGGAG
    CAGCCCCCTT CCGTCTTGCT GTCCCCCTCC CTCCAGTATG CTGGGGCCGA GACCCTAGCG
    TCCAAAGTGC AATTCCGGGA TCCCAAGTCC CAGCGAACGC ACCAGGCCCA GGTGGCGTTC
    CAGGTGTGCG TGCGCCCGGG CTCCTACACC CCCGGACCCC CTTCGGCCAC CCTCCGAGAA
    CCTCCCGACC CTCACTTCAG CCCCGCAGAA CTCGAGTGGA TAACCAAGGA GAAGGGGGCC
    ACACTCCTCT ATGCCCTGCT GGTGCGGGTG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288100
    Clone ID Related Accession (Same CDS sequence) XM_022418485.1
    Accession Version XM_022418485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGTGG GGGAGCAGGC AGGGGACACT
    GTGGGCGTGG TGCGGCGGGA GGACGGGACC CTGCACTTCT TCGTCAACGG GATGACCCAG
    GGCCCTGCGG CCTGGAATGT GCCCCCTGGC GTCTACGCTG TTGTGGACCT CTATGGCCAG
    GCAGCCCAAG CTACCATTGT GGATGACGTG GAGGTGCCCC CGGCCCCTGA GCCCCTCTCT
    GAAGGGAACA ACCAGGTGTC TCCCAGCTCC CCATCATCAG GGGCCGGGGG CTCTGACCTG
    CGCTTCCATC AGCTGCACGG CAGTAATGCA GTCATCACCA ACGGGGGCCG CACCGCACTC
    CGGCACAACT GCCGCAGCGA GTTCAATGAC GCCATCGTCA TCTCCAACCG GGCCCTGCGG
    GACGGAGAGC TGTTTGAAAT CGTTATTCAG AAGATGGTGG ACCGCTGGTC AGGCTCCATT
    GAGGCTGGAG TGACTGCCAT TCGGCCGGAA GACCTCGAAT TCCCCAACAC CATGACAGAC
    ATTGACTACG ACACATGGAT GCTGAGTGGC ACGGCCATCA TGCAAGACGG GAACACGATG
    CGCAACAACT ACGGGTGTGA CCTGGACGCC CTGGGCACGG GTGCACGCAT CGGCATGATG
    CGAACAGCCA AGGGTGACCT GCACTACTTC ATCAACGGCC AGGACCAAGG CGCCGCCTGC
    TCAGGCTTGC CTCCGGGTAA AGAGGTGTAC GCAGTAGTGG ATCTTTACGG CCAGTGTGTC
    CAAGTGTCCA TCACCAATGC CACCGGCCCC ATGGACAACA GCTTGGCAAC CAGCAACACT
    GCCACCGAGA AATCCTTCCC TTTGCACTCC CCAGCGGCTG GCGTGGCTCA CCGATTCCAC
    AGTACTTGCG GCAAGAATGT GGCCCTGGAG GAGGATGGCA CAAGGGCGGT ACGTGCGGTG
    GGCTATGCGC ATGGCCTGGT CTTCAGCACC AAGGAACTCA AGACGGAGGA AGTATTTGAG
    GTGAAAGTAG AGGAACTGGA TGAGAAGTGG GCGGGCTCCC TCCGGCTGGG GCTGACCACA
    CTAGCGCCCG GGGAGATGGG GCCTGGCGCA GGTGGGGGCC CAGGGCTGCC TCCCTCCCTC
    CCGGAGCTCC GGACAAAGAC CACCTGGATG GTGTCCAGCT GTGAAGTGAG GCGTGACGGG
    CAGCTCCAGA GGATGAACTA CGGCCGGAGC CTGGAGAGGC TGGGGGTGGG GAGCCGCGTG
    GGCATTCGTC GGGGCGCAGA TGACACGATG CACATCCTGG TGGATGGAGA GGACATGGGG
    CCCGCAGCTA CCGGCATTGC CAAGAATGTG TGGGCCGTGC TGGATCTCTA CGGGCCGGTA
    CGGAGTGTGT CTGTGGTCAG CTCCACAAGG CTGGAGGAGC CCGAGGGCAC CCAGCCTCCA
    TCTCCCAGCT CGGACACGGG CAGCGAGGCC GAGGAAGATG ACGATGGGGA GGAGCACAGC
    TTGAGAGGTC AGAGTCAAGT GGCCATGATG TCGACGTCAC TGGAGTTCCT GGAGAACCAT
    GGGAAGAATA TCCTTTTGTC CAACGGGAAC CGTACGGCCA CACGCGTAGC CAGCTACAAC
    CAGGGCATCG TTGTCATCAA CCAGCCCCTG GTGCCCCAGC TCCTAGTCCA GGTGCGGATA
    GACTTCCTGA ACCGGCAGTG GACATCTTCC CTGGTCCTGG GAGTCATCAC CTGCCCGCCT
    GAGAGGCTCA ACTTCCCTGC TTCTGCCTGC GCCCTCAAAC GGGCAGCCTG GCTGCTGCGG
    GGCCGGGGCA TCTTCCACAA TGGTCTCAAG ATTTGTGAGA AGTTTGGGCC AAATCTGGAC
    ACGTGTCCTG AAGGCACCAT CCTGGGGCTG CGGCTAGACA GCTCTGGGGG CCTGCATCTC
    CACGTCAATG GGATGGACCA GGGGGTGGCG GTATCAGATG TCCCCCAGCC CTGCCATGCG
    CTTGTGGACC TCTATGGGCA GTGTGAGCAG GTGACAATCG TGAGTCCTGA ACCAGGGGCA
    GCCAGTGGGA AGAGTGCTGG AACCCAAGGG GACATGGAGA AAGCGGACAT GGTGGATGGT
    ATCAAGGAAA GTGTGTGCTG GGGCCCCCTG CCAGCTGCCA GCCCTCTCAA GAGCTGCGAG
    TACCACGCCC TCTGCTCCCG CTTCCAAGAA CTGCTGTTGC TTCCTGAGGA CTATTTCATG
    CCTCCACCCA AGCGGAGCCT GTGCTACTGT GAGTCTTGCC GGAAGCTTCG AGGAGGCTCC
    CAAGAGGGTG CGTGGAAGAA GCCACAGAAC CTTTACTGGT CGAGGGGAGG ATCTTTGTCC
    AGTCTGCCCG GTCTACACCC TAGGGTGAAT CCCCGCCTGG AAGCTGGAAC GATGACTAAG
    AAGTGGCACA TGGCATATCA CGGGAGCAAC GTGGCAGCCA TCCGGAGGGT GCTAGACCGA
    GGAGAGCTGG GAGCAGGCAC CGCCTCCATC CTGAGCTGCC GGCCCTTGAA AGGCAAGCCT
    GGGGTGGGGT TTGAGGAGCC TGGCGAGAAC TGCGCCCCAC CCCGGGAGGA GCAGCCCCCT
    TCCGTCTTGC TGTCCCCCTC CCTCCAGTAT GCTGGGGCCG AGACCCTAGC GTCCAAAGTG
    CAATTCCGGG ATCCCAAGTC CCAGCGAACG CACCAGGCCC AGGTGGCGTT CCAGGTGTGC
    GTGCGCCCGG GCTCCTACAC CCCCGGACCC CCTTCGGCCA CCCTCCGAGA ACCTCCCGAC
    CCTCACTTCA GCCCCGCAGA ACTCGAGTGG ATAACCAAGG AGAAGGGGGC CACACTCCTC
    TATGCCCTGC TGGTGCGGGT GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274193.1
    CDS145..4911
    Translation

    Target ORF information:

    RefSeq Version XM_022418485.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGTGG GGGAGCAGGC AGGGGACACT
    GTGGGCGTGG TGCGGCGGGA GGACGGGACC CTGCACTTCT TCGTCAACGG GATGACCCAG
    GGCCCTGCGG CCTGGAATGT GCCCCCTGGC GTCTACGCTG TTGTGGACCT CTATGGCCAG
    GCAGCCCAAG CTACCATTGT GGATGACGTG GAGGTGCCCC CGGCCCCTGA GCCCCTCTCT
    GAAGGGAACA ACCAGGTGTC TCCCAGCTCC CCATCATCAG GGGCCGGGGG CTCTGACCTG
    CGCTTCCATC AGCTGCACGG CAGTAATGCA GTCATCACCA ACGGGGGCCG CACCGCACTC
    CGGCACAACT GCCGCAGCGA GTTCAATGAC GCCATCGTCA TCTCCAACCG GGCCCTGCGG
    GACGGAGAGC TGTTTGAAAT CGTTATTCAG AAGATGGTGG ACCGCTGGTC AGGCTCCATT
    GAGGCTGGAG TGACTGCCAT TCGGCCGGAA GACCTCGAAT TCCCCAACAC CATGACAGAC
    ATTGACTACG ACACATGGAT GCTGAGTGGC ACGGCCATCA TGCAAGACGG GAACACGATG
    CGCAACAACT ACGGGTGTGA CCTGGACGCC CTGGGCACGG GTGCACGCAT CGGCATGATG
    CGAACAGCCA AGGGTGACCT GCACTACTTC ATCAACGGCC AGGACCAAGG CGCCGCCTGC
    TCAGGCTTGC CTCCGGGTAA AGAGGTGTAC GCAGTAGTGG ATCTTTACGG CCAGTGTGTC
    CAAGTGTCCA TCACCAATGC CACCGGCCCC ATGGACAACA GCTTGGCAAC CAGCAACACT
    GCCACCGAGA AATCCTTCCC TTTGCACTCC CCAGCGGCTG GCGTGGCTCA CCGATTCCAC
    AGTACTTGCG GCAAGAATGT GGCCCTGGAG GAGGATGGCA CAAGGGCGGT ACGTGCGGTG
    GGCTATGCGC ATGGCCTGGT CTTCAGCACC AAGGAACTCA AGACGGAGGA AGTATTTGAG
    GTGAAAGTAG AGGAACTGGA TGAGAAGTGG GCGGGCTCCC TCCGGCTGGG GCTGACCACA
    CTAGCGCCCG GGGAGATGGG GCCTGGCGCA GGTGGGGGCC CAGGGCTGCC TCCCTCCCTC
    CCGGAGCTCC GGACAAAGAC CACCTGGATG GTGTCCAGCT GTGAAGTGAG GCGTGACGGG
    CAGCTCCAGA GGATGAACTA CGGCCGGAGC CTGGAGAGGC TGGGGGTGGG GAGCCGCGTG
    GGCATTCGTC GGGGCGCAGA TGACACGATG CACATCCTGG TGGATGGAGA GGACATGGGG
    CCCGCAGCTA CCGGCATTGC CAAGAATGTG TGGGCCGTGC TGGATCTCTA CGGGCCGGTA
    CGGAGTGTGT CTGTGGTCAG CTCCACAAGG CTGGAGGAGC CCGAGGGCAC CCAGCCTCCA
    TCTCCCAGCT CGGACACGGG CAGCGAGGCC GAGGAAGATG ACGATGGGGA GGAGCACAGC
    TTGAGAGGTC AGAGTCAAGT GGCCATGATG TCGACGTCAC TGGAGTTCCT GGAGAACCAT
    GGGAAGAATA TCCTTTTGTC CAACGGGAAC CGTACGGCCA CACGCGTAGC CAGCTACAAC
    CAGGGCATCG TTGTCATCAA CCAGCCCCTG GTGCCCCAGC TCCTAGTCCA GGTGCGGATA
    GACTTCCTGA ACCGGCAGTG GACATCTTCC CTGGTCCTGG GAGTCATCAC CTGCCCGCCT
    GAGAGGCTCA ACTTCCCTGC TTCTGCCTGC GCCCTCAAAC GGGCAGCCTG GCTGCTGCGG
    GGCCGGGGCA TCTTCCACAA TGGTCTCAAG ATTTGTGAGA AGTTTGGGCC AAATCTGGAC
    ACGTGTCCTG AAGGCACCAT CCTGGGGCTG CGGCTAGACA GCTCTGGGGG CCTGCATCTC
    CACGTCAATG GGATGGACCA GGGGGTGGCG GTATCAGATG TCCCCCAGCC CTGCCATGCG
    CTTGTGGACC TCTATGGGCA GTGTGAGCAG GTGACAATCG TGAGTCCTGA ACCAGGGGCA
    GCCAGTGGGA AGAGTGCTGG AACCCAAGGG GACATGGAGA AAGCGGACAT GGTGGATGGT
    ATCAAGGAAA GTGTGTGCTG GGGCCCCCTG CCAGCTGCCA GCCCTCTCAA GAGCTGCGAG
    TACCACGCCC TCTGCTCCCG CTTCCAAGAA CTGCTGTTGC TTCCTGAGGA CTATTTCATG
    CCTCCACCCA AGCGGAGCCT GTGCTACTGT GAGTCTTGCC GGAAGCTTCG AGGAGGCTCC
    CAAGAGGGTG CGTGGAAGAA GCCACAGAAC CTTTACTGGT CGAGGGGAGG ATCTTTGTCC
    AGTCTGCCCG GTCTACACCC TAGGGTGAAT CCCCGCCTGG AAGCTGGAAC GATGACTAAG
    AAGTGGCACA TGGCATATCA CGGGAGCAAC GTGGCAGCCA TCCGGAGGGT GCTAGACCGA
    GGAGAGCTGG GAGCAGGCAC CGCCTCCATC CTGAGCTGCC GGCCCTTGAA AGGCAAGCCT
    GGGGTGGGGT TTGAGGAGCC TGGCGAGAAC TGCGCCCCAC CCCGGGAGGA GCAGCCCCCT
    TCCGTCTTGC TGTCCCCCTC CCTCCAGTAT GCTGGGGCCG AGACCCTAGC GTCCAAAGTG
    CAATTCCGGG ATCCCAAGTC CCAGCGAACG CACCAGGCCC AGGTGGCGTT CCAGGTGTGC
    GTGCGCCCGG GCTCCTACAC CCCCGGACCC CCTTCGGCCA CCCTCCGAGA ACCTCCCGAC
    CCTCACTTCA GCCCCGCAGA ACTCGAGTGG ATAACCAAGG AGAAGGGGGC CACACTCCTC
    TATGCCCTGC TGGTGCGGGT GGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288101
    Clone ID Related Accession (Same CDS sequence) XM_022418486.1
    Accession Version XM_022418486.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGTGG GGGAGCAGGC AGGGGACACT
    GTGGGCGTGG TGCGGCGGGA GGACGGGACC CTGCACTTCT TCGTCAACGG GATGACCCAG
    GGCCCTGCGG CCTGGAATGT GCCCCCTGGC GTCTACGCTG TTGTGGACCT CTATGGCCAG
    GCAGCCCAAG CTACCATTGT GGATGACGTG GAGGTGCCCC CGGCCCCTGA GCCCCTCTCT
    GAAGGGAACA ACCAGGTGTC TCCCAGCTCC CCATCATCAG GGGCCGGGGG CTCTGACCTG
    CGCTTCCATC AGCTGCACGG CAGTAATGCA GTCATCACCA ACGGGGGCCG CACCGCACTC
    CGGCACAACT GCCGCAGCGA GTTCAATGAC GCCATCGTCA TCTCCAACCG GGCCCTGCGG
    GACGGAGAGC TGTTTGAAAT CGTTATTCAG AAGATGGTGG ACCGCTGGTC AGGCTCCATT
    GAGGCTGGAG TGACTGCCAT TCGGCCGGAA GACCTCGAAT TCCCCAACAC CATGACAGAC
    ATTGACTACG ACACATGGAT GCTGAGTGGC ACGGCCATCA TGCAAGACGG GAACACGATG
    CGCAACAACT ACGGGTGTGA CCTGGACGCC CTGGGCACGG GTGCACGCAT CGGCATGATG
    CGAACAGCCA AGGGTGACCT GCACTACTTC ATCAACGGCC AGGACCAAGG CGCCGCCTGC
    TCAGGCTTGC CTCCGGAGGT GTACGCAGTA GTGGATCTTT ACGGCCAGTG TGTCCAAGTG
    TCCATCACCA ATGCCACCGG CCCCATGGAC AACAGCTTGG CAACCAGCAA CACTGCCACC
    GAGAAATCCT TCCCTTTGCA CTCCCCAGCG GCTGGCGTGG CTCACCGATT CCACAGTACT
    TGCGGCAAGA ATGTGGCCCT GGAGGAGGAT GGCACAAGGG CGGTACGTGC GGTGGGCTAT
    GCGCATGGCC TGGTCTTCAG CACCAAGGAA CTCAAGACGG AGGAAGTATT TGAGGTGAAA
    GTAGAGGAAC TGGATGAGAA GTGGGCGGGC TCCCTCCGGC TGGGGCTGAC CACACTAGCG
    CCCGGGGAGA TGGGGCCTGG CGCAGGTGGG GGCCCAGGGC TGCCTCCCTC CCTCCCGGAG
    CTCCGGACAA AGACCACCTG GATGGTGTCC AGCTGTGAAG TGAGGCGTGA CGGGCAGCTC
    CAGAGGATGA ACTACGGCCG GAGCCTGGAG AGGCTGGGGG TGGGGAGCCG CGTGGGCATT
    CGTCGGGGCG CAGATGACAC GATGCACATC CTGGTGGATG GAGAGGACAT GGGGCCCGCA
    GCTACCGGCA TTGCCAAGAA TGTGTGGGCC GTGCTGGATC TCTACGGGCC GGTACGGAGT
    GTGTCTGTGG TCAGCTCCAC AAGGCTGGAG GAGCCCGAGG GCACCCAGCC TCCATCTCCC
    AGCTCGGACA CGGGCAGCGA GGCCGAGGAA GATGACGATG GGGAGGAGCA CAGCTTGAGA
    GGTCAGAGTC AAGTGGCCAT GATGTCGACG TCACTGGAGT TCCTGGAGAA CCATGGGAAG
    AATATCCTTT TGTCCAACGG GAACCGTACG GCCACACGCG TAGCCAGCTA CAACCAGGGC
    ATCGTTGTCA TCAACCAGCC CCTGGTGCCC CAGCTCCTAG TCCAGGTGCG GATAGACTTC
    CTGAACCGGC AGTGGACATC TTCCCTGGTC CTGGGAGTCA TCACCTGCCC GCCTGAGAGG
    CTCAACTTCC CTGCTTCTGC CTGCGCCCTC AAACGGGCAG CCTGGCTGCT GCGGGGCCGG
    GGCATCTTCC ACAATGGTCT CAAGATTTGT GAGAAGTTTG GGCCAAATCT GGACACGTGT
    CCTGAAGGCA CCATCCTGGG GCTGCGGCTA GACAGCTCTG GGGGCCTGCA TCTCCACGTC
    AATGGGATGG ACCAGGGGGT GGCGGTATCA GATGTCCCCC AGCCCTGCCA TGCGCTTGTG
    GACCTCTATG GGCAGTGTGA GCAGGTGACA ATCGTGAGTC CTGAACCAGG GGCAGCCAGT
    GGGAAGAGTG CTGGAACCCA AGGGGACATG GAGAAAGCGG ACATGGTGGA TGGTATCAAG
    GAAAGTGTGT GCTGGGGCCC CCTGCCAGCT GCCAGCCCTC TCAAGAGCTG CGAGTACCAC
    GCCCTCTGCT CCCGCTTCCA AGAACTGCTG TTGCTTCCTG AGGACTATTT CATGCCTCCA
    CCCAAGCGGA GCCTGTGCTA CTGTGAGTCT TGCCGGAAGC TTCGAGGAGG CTCCCAAGAG
    GGTGCGTGGA AGAAGCCACA GAACCTTTAC TGGTCGAGGG GAGGATCTTT GTCCAGTCTG
    CCCGGTCTAC ACCCTAGGGT GAATCCCCGC CTGGAAGCTG GAACGATGAC TAAGAAGTGG
    CACATGGCAT ATCACGGGAG CAACGTGGCA GCCATCCGGA GGGTGCTAGA CCGAGGAGAG
    CTGGGAGCAG GCACCGCCTC CATCCTGAGC TGCCGGCCCT TGAAAGGCAA GCCTGGGGTG
    GGGTTTGAGG AGCCTGGCGA GAACTGCGCC CCACCCCGGG AGGAGCAGCC CCCTTCCGTC
    TTGCTGTCCC CCTCCCTCCA GTATGCTGGG GCCGAGACCC TAGCGTCCAA AGTGCAATTC
    CGGGATCCCA AGTCCCAGCG AACGCACCAG GCCCAGGTGG CGTTCCAGGT GTGCGTGCGC
    CCGGGCTCCT ACACCCCCGG ACCCCCTTCG GCCACCCTCC GAGAACCTCC CGACCCTCAC
    TTCAGCCCCG CAGAACTCGA GTGGATAACC AAGGAGAAGG GGGCCACACT CCTCTATGCC
    CTGCTGGTGC GGGTGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274194.1
    CDS145..4905
    Translation

    Target ORF information:

    RefSeq Version XM_022418486.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418486.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGTGG GGGAGCAGGC AGGGGACACT
    GTGGGCGTGG TGCGGCGGGA GGACGGGACC CTGCACTTCT TCGTCAACGG GATGACCCAG
    GGCCCTGCGG CCTGGAATGT GCCCCCTGGC GTCTACGCTG TTGTGGACCT CTATGGCCAG
    GCAGCCCAAG CTACCATTGT GGATGACGTG GAGGTGCCCC CGGCCCCTGA GCCCCTCTCT
    GAAGGGAACA ACCAGGTGTC TCCCAGCTCC CCATCATCAG GGGCCGGGGG CTCTGACCTG
    CGCTTCCATC AGCTGCACGG CAGTAATGCA GTCATCACCA ACGGGGGCCG CACCGCACTC
    CGGCACAACT GCCGCAGCGA GTTCAATGAC GCCATCGTCA TCTCCAACCG GGCCCTGCGG
    GACGGAGAGC TGTTTGAAAT CGTTATTCAG AAGATGGTGG ACCGCTGGTC AGGCTCCATT
    GAGGCTGGAG TGACTGCCAT TCGGCCGGAA GACCTCGAAT TCCCCAACAC CATGACAGAC
    ATTGACTACG ACACATGGAT GCTGAGTGGC ACGGCCATCA TGCAAGACGG GAACACGATG
    CGCAACAACT ACGGGTGTGA CCTGGACGCC CTGGGCACGG GTGCACGCAT CGGCATGATG
    CGAACAGCCA AGGGTGACCT GCACTACTTC ATCAACGGCC AGGACCAAGG CGCCGCCTGC
    TCAGGCTTGC CTCCGGAGGT GTACGCAGTA GTGGATCTTT ACGGCCAGTG TGTCCAAGTG
    TCCATCACCA ATGCCACCGG CCCCATGGAC AACAGCTTGG CAACCAGCAA CACTGCCACC
    GAGAAATCCT TCCCTTTGCA CTCCCCAGCG GCTGGCGTGG CTCACCGATT CCACAGTACT
    TGCGGCAAGA ATGTGGCCCT GGAGGAGGAT GGCACAAGGG CGGTACGTGC GGTGGGCTAT
    GCGCATGGCC TGGTCTTCAG CACCAAGGAA CTCAAGACGG AGGAAGTATT TGAGGTGAAA
    GTAGAGGAAC TGGATGAGAA GTGGGCGGGC TCCCTCCGGC TGGGGCTGAC CACACTAGCG
    CCCGGGGAGA TGGGGCCTGG CGCAGGTGGG GGCCCAGGGC TGCCTCCCTC CCTCCCGGAG
    CTCCGGACAA AGACCACCTG GATGGTGTCC AGCTGTGAAG TGAGGCGTGA CGGGCAGCTC
    CAGAGGATGA ACTACGGCCG GAGCCTGGAG AGGCTGGGGG TGGGGAGCCG CGTGGGCATT
    CGTCGGGGCG CAGATGACAC GATGCACATC CTGGTGGATG GAGAGGACAT GGGGCCCGCA
    GCTACCGGCA TTGCCAAGAA TGTGTGGGCC GTGCTGGATC TCTACGGGCC GGTACGGAGT
    GTGTCTGTGG TCAGCTCCAC AAGGCTGGAG GAGCCCGAGG GCACCCAGCC TCCATCTCCC
    AGCTCGGACA CGGGCAGCGA GGCCGAGGAA GATGACGATG GGGAGGAGCA CAGCTTGAGA
    GGTCAGAGTC AAGTGGCCAT GATGTCGACG TCACTGGAGT TCCTGGAGAA CCATGGGAAG
    AATATCCTTT TGTCCAACGG GAACCGTACG GCCACACGCG TAGCCAGCTA CAACCAGGGC
    ATCGTTGTCA TCAACCAGCC CCTGGTGCCC CAGCTCCTAG TCCAGGTGCG GATAGACTTC
    CTGAACCGGC AGTGGACATC TTCCCTGGTC CTGGGAGTCA TCACCTGCCC GCCTGAGAGG
    CTCAACTTCC CTGCTTCTGC CTGCGCCCTC AAACGGGCAG CCTGGCTGCT GCGGGGCCGG
    GGCATCTTCC ACAATGGTCT CAAGATTTGT GAGAAGTTTG GGCCAAATCT GGACACGTGT
    CCTGAAGGCA CCATCCTGGG GCTGCGGCTA GACAGCTCTG GGGGCCTGCA TCTCCACGTC
    AATGGGATGG ACCAGGGGGT GGCGGTATCA GATGTCCCCC AGCCCTGCCA TGCGCTTGTG
    GACCTCTATG GGCAGTGTGA GCAGGTGACA ATCGTGAGTC CTGAACCAGG GGCAGCCAGT
    GGGAAGAGTG CTGGAACCCA AGGGGACATG GAGAAAGCGG ACATGGTGGA TGGTATCAAG
    GAAAGTGTGT GCTGGGGCCC CCTGCCAGCT GCCAGCCCTC TCAAGAGCTG CGAGTACCAC
    GCCCTCTGCT CCCGCTTCCA AGAACTGCTG TTGCTTCCTG AGGACTATTT CATGCCTCCA
    CCCAAGCGGA GCCTGTGCTA CTGTGAGTCT TGCCGGAAGC TTCGAGGAGG CTCCCAAGAG
    GGTGCGTGGA AGAAGCCACA GAACCTTTAC TGGTCGAGGG GAGGATCTTT GTCCAGTCTG
    CCCGGTCTAC ACCCTAGGGT GAATCCCCGC CTGGAAGCTG GAACGATGAC TAAGAAGTGG
    CACATGGCAT ATCACGGGAG CAACGTGGCA GCCATCCGGA GGGTGCTAGA CCGAGGAGAG
    CTGGGAGCAG GCACCGCCTC CATCCTGAGC TGCCGGCCCT TGAAAGGCAA GCCTGGGGTG
    GGGTTTGAGG AGCCTGGCGA GAACTGCGCC CCACCCCGGG AGGAGCAGCC CCCTTCCGTC
    TTGCTGTCCC CCTCCCTCCA GTATGCTGGG GCCGAGACCC TAGCGTCCAA AGTGCAATTC
    CGGGATCCCA AGTCCCAGCG AACGCACCAG GCCCAGGTGG CGTTCCAGGT GTGCGTGCGC
    CCGGGCTCCT ACACCCCCGG ACCCCCTTCG GCCACCCTCC GAGAACCTCC CGACCCTCAC
    TTCAGCCCCG CAGAACTCGA GTGGATAACC AAGGAGAAGG GGGCCACACT CCTCTATGCC
    CTGCTGGTGC GGGTGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288102
    Clone ID Related Accession (Same CDS sequence) XM_022418487.1
    Accession Version XM_022418487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGTGG GGGAGCAGGC AGGGGACACT
    GTGGGCGTGG TGCGGCGGGA GGACGGGACC CTGCACTTCT TCGTCAACGG GATGACCCAG
    GGCCCTGCGG CCTGGAATGT GCCCCCTGGC GTCTACGCTG TTGTGGACCT CTATGGCCAG
    GCAGCCCAAG CTACCATTGT GGATGACGTG GAGGTGCCCC CGGCCCCTGA GCCCCTCTCT
    GAAGGGAACA ACCAGGTGTC TCCCAGCTCC CCATCATCAG GGGCCGGGGG CTCTGACCTG
    CGCTTCCATC AGCTGCACGG CAGTAATGCA GTCATCACCA ACGGGGGCCG CACCGCACTC
    CGGCACAACT GCCGCAGCGA GTTCAATGAC GCCATCGTCA TCTCCAACCG GGCCCTGCGG
    GACGGAGAGC TGTTTGAAAT CGTTATTCAG AAGATGGTGG ACCGCTGGTC AGGCTCCATT
    GAGGCTGGAG TGACTGCCAT TCGGCCGGAA GACCTCGAAT TCCCCAACAC CATGACAGAC
    ATTGACTACG ACACATGGAT GCTGAGTGGC ACGGCCATCA TGCAAGACGG GAACACGATG
    CGCAACAACT ACGGGTGTGA CCTGGACGCC CTGGGCACGG GTGCACGCAT CGGCATGATG
    CGAACAGCCA AGGGTGACCT GCACTACTTC ATCAACGGCC AGGACCAAGG CGCCGCCTGC
    TCAGGCTTGC CTCCGGGTAA AGAGGTGTAC GCAGTAGTGG ATCTTTACGG CCAGTGTGTC
    CAAGTGTCCA TCACCAATGC CACCGGCCCC ATGGACAACA GCTTGGCAAC CAGCAACACT
    GCCACCGAGA AATCCTTCCC TTTGCACTCC CCAGCGGCTG GCGTGGCTCA CCGATTCCAC
    AGTACTTGCG GCAAGAATGT GGCCCTGGAG GAGGATGGCA CAAGGGCGGT ACGTGCGGTG
    GGCTATGCGC ATGGCCTGGT CTTCAGCACC AAGGAACTCA AGACGGAGGA AGTATTTGAG
    GTGAAAGTAG AGGAACTGGA TGAGAAGTGG GCGGGCTCCC TCCGGCTGGG GCTGACCACA
    CTAGCGCCCG GGGAGATGGG GCCTGGCGCA GGTGGGGGCC CAGGGCTGCC TCCCTCCCTC
    CCGGAGCTCC GGACAAAGAC CACCTGGATG GTGTCCAGCT GTGAAGTGAG GCGTGACGGG
    CAGCTCCAGA GGATGAACTA CGGCCGGAGC CTGGAGAGGC TGGGGGTGGG GAGCCGCGTG
    GGCATTCGTC GGGGCGCAGA TGACACGATG CACATCCTGG TGGATGGAGA GGACATGGGG
    CCCGCAGCTA CCGGCATTGC CAAGAATGTG TGGGCCGTGC TGGATCTCTA CGGGCCGGTA
    CGGAGTGTGT CTGTGGTCAG CTCCACAAGG CTGGAGGAGC CCGAGGGCAC CCAGCCTCCA
    TCTCCCAGCT CGGACACGGG CAGCGAGGCC GAGGAAGATG ACGATGGGGA GGAGCACAGC
    TTGAGAGGTC AGAGTCAAGT GGCCATGATG TCGACGTCAC TGGAGTTCCT GGAGAACCAT
    GGGAAGAATA TCCTTTTGTC CAACGGGAAC CGTACGGCCA CACGCGTAGC CAGCTACAAC
    CAGGGCATCG TTGTCATCAA CCAGCCCCTG GTGCCCCAGC TCCTAGTCCA GGTGCGGATA
    GACTTCCTGA ACCGGCAGTG GACATCTTCC CTGGTCCTGG GAGTCATCAC CTGCCCGCCT
    GAGAGGCTCA ACTTCCCTGC TTCTGCCTGC GCCCTCAAAC GGGCAGCCTG GCTGCTGCGG
    GGCCGGGGCA TCTTCCACAA TGGTCTCAAG ATTTGTGAGA AGTTTGGGCC AAATCTGGAC
    ACGTGTCCTG AAGGCACCAT CCTGGGGCTG CGGCTAGACA GCTCTGGGGG CCTGCATCTC
    CACGTCAATG GGATGGACCA GGGGGTGGCG GTATCAGATG TCCCCCAGCC CTGCCATGCG
    CTTGTGGACC TCTATGGGCA GTGTGAGCAG GTGACAATCG TGAGTCCTGA ACCAGGGGCA
    GCCAGTGGGA AGAGTGCTGG AACCCAAGGG GACATGGAGA AAGCGGACAT GGTGGATGGT
    ATCAAGGAAA GTGTGTGCTG GGGCCCCCTG CCAGCTGCCA GCCCTCTCAA GAGCTGCGAG
    TACCACGCCC TCTGCTCCCG CTTCCAAGAA CTGCTGTTGC TTCCTGAGGA CTATTTCATG
    CCTCCACCCA AGCGGAGCCT GTGCTACTGT GAGTCTTGCC GGAAGCTTCG AGGTGACGAG
    GCCCACAGGC GCCGTGGGGA GCCCCCCCGG GAATACGCGC TGCCCTTCGG CTGGTGCAGG
    TTCAATCTCA GGGTGAATCC CCGCCTGGAA GCTGGAACGA TGACTAAGAA GTGGCACATG
    GCATATCACG GGAGCAACGT GGCAGCCATC CGGAGGGTGC TAGACCGAGG AGAGCTGGGA
    GCAGGCACCG CCTCCATCCT GAGCTGCCGG CCCTTGAAAG GCAAGCCTGG GGTGGGGTTT
    GAGGAGCCTG GCGAGAACTG CGCCCCACCC CGGGAGGAGC AGCCCCCTTC CGTCTTGCTG
    TCCCCCTCCC TCCAGTATGC TGGGGCCGAG ACCCTAGCGT CCAAAGTGCA ATTCCGGGAT
    CCCAAGTCCC AGCGAACGCA CCAGGCCCAG GTGGCGTTCC AGGTGTGCGT GCGCCCGGGC
    TCCTACACCC CCGGACCCCC TTCGGCCACC CTCCGAGAAC CTCCCGACCC TCACTTCAGC
    CCCGCAGAAC TCGAGTGGAT AACCAAGGAG AAGGGGGCCA CACTCCTCTA TGCCCTGCTG
    GTGCGGGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274195.1
    CDS145..4899
    Translation

    Target ORF information:

    RefSeq Version XM_022418487.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGTGG GGGAGCAGGC AGGGGACACT
    GTGGGCGTGG TGCGGCGGGA GGACGGGACC CTGCACTTCT TCGTCAACGG GATGACCCAG
    GGCCCTGCGG CCTGGAATGT GCCCCCTGGC GTCTACGCTG TTGTGGACCT CTATGGCCAG
    GCAGCCCAAG CTACCATTGT GGATGACGTG GAGGTGCCCC CGGCCCCTGA GCCCCTCTCT
    GAAGGGAACA ACCAGGTGTC TCCCAGCTCC CCATCATCAG GGGCCGGGGG CTCTGACCTG
    CGCTTCCATC AGCTGCACGG CAGTAATGCA GTCATCACCA ACGGGGGCCG CACCGCACTC
    CGGCACAACT GCCGCAGCGA GTTCAATGAC GCCATCGTCA TCTCCAACCG GGCCCTGCGG
    GACGGAGAGC TGTTTGAAAT CGTTATTCAG AAGATGGTGG ACCGCTGGTC AGGCTCCATT
    GAGGCTGGAG TGACTGCCAT TCGGCCGGAA GACCTCGAAT TCCCCAACAC CATGACAGAC
    ATTGACTACG ACACATGGAT GCTGAGTGGC ACGGCCATCA TGCAAGACGG GAACACGATG
    CGCAACAACT ACGGGTGTGA CCTGGACGCC CTGGGCACGG GTGCACGCAT CGGCATGATG
    CGAACAGCCA AGGGTGACCT GCACTACTTC ATCAACGGCC AGGACCAAGG CGCCGCCTGC
    TCAGGCTTGC CTCCGGGTAA AGAGGTGTAC GCAGTAGTGG ATCTTTACGG CCAGTGTGTC
    CAAGTGTCCA TCACCAATGC CACCGGCCCC ATGGACAACA GCTTGGCAAC CAGCAACACT
    GCCACCGAGA AATCCTTCCC TTTGCACTCC CCAGCGGCTG GCGTGGCTCA CCGATTCCAC
    AGTACTTGCG GCAAGAATGT GGCCCTGGAG GAGGATGGCA CAAGGGCGGT ACGTGCGGTG
    GGCTATGCGC ATGGCCTGGT CTTCAGCACC AAGGAACTCA AGACGGAGGA AGTATTTGAG
    GTGAAAGTAG AGGAACTGGA TGAGAAGTGG GCGGGCTCCC TCCGGCTGGG GCTGACCACA
    CTAGCGCCCG GGGAGATGGG GCCTGGCGCA GGTGGGGGCC CAGGGCTGCC TCCCTCCCTC
    CCGGAGCTCC GGACAAAGAC CACCTGGATG GTGTCCAGCT GTGAAGTGAG GCGTGACGGG
    CAGCTCCAGA GGATGAACTA CGGCCGGAGC CTGGAGAGGC TGGGGGTGGG GAGCCGCGTG
    GGCATTCGTC GGGGCGCAGA TGACACGATG CACATCCTGG TGGATGGAGA GGACATGGGG
    CCCGCAGCTA CCGGCATTGC CAAGAATGTG TGGGCCGTGC TGGATCTCTA CGGGCCGGTA
    CGGAGTGTGT CTGTGGTCAG CTCCACAAGG CTGGAGGAGC CCGAGGGCAC CCAGCCTCCA
    TCTCCCAGCT CGGACACGGG CAGCGAGGCC GAGGAAGATG ACGATGGGGA GGAGCACAGC
    TTGAGAGGTC AGAGTCAAGT GGCCATGATG TCGACGTCAC TGGAGTTCCT GGAGAACCAT
    GGGAAGAATA TCCTTTTGTC CAACGGGAAC CGTACGGCCA CACGCGTAGC CAGCTACAAC
    CAGGGCATCG TTGTCATCAA CCAGCCCCTG GTGCCCCAGC TCCTAGTCCA GGTGCGGATA
    GACTTCCTGA ACCGGCAGTG GACATCTTCC CTGGTCCTGG GAGTCATCAC CTGCCCGCCT
    GAGAGGCTCA ACTTCCCTGC TTCTGCCTGC GCCCTCAAAC GGGCAGCCTG GCTGCTGCGG
    GGCCGGGGCA TCTTCCACAA TGGTCTCAAG ATTTGTGAGA AGTTTGGGCC AAATCTGGAC
    ACGTGTCCTG AAGGCACCAT CCTGGGGCTG CGGCTAGACA GCTCTGGGGG CCTGCATCTC
    CACGTCAATG GGATGGACCA GGGGGTGGCG GTATCAGATG TCCCCCAGCC CTGCCATGCG
    CTTGTGGACC TCTATGGGCA GTGTGAGCAG GTGACAATCG TGAGTCCTGA ACCAGGGGCA
    GCCAGTGGGA AGAGTGCTGG AACCCAAGGG GACATGGAGA AAGCGGACAT GGTGGATGGT
    ATCAAGGAAA GTGTGTGCTG GGGCCCCCTG CCAGCTGCCA GCCCTCTCAA GAGCTGCGAG
    TACCACGCCC TCTGCTCCCG CTTCCAAGAA CTGCTGTTGC TTCCTGAGGA CTATTTCATG
    CCTCCACCCA AGCGGAGCCT GTGCTACTGT GAGTCTTGCC GGAAGCTTCG AGGTGACGAG
    GCCCACAGGC GCCGTGGGGA GCCCCCCCGG GAATACGCGC TGCCCTTCGG CTGGTGCAGG
    TTCAATCTCA GGGTGAATCC CCGCCTGGAA GCTGGAACGA TGACTAAGAA GTGGCACATG
    GCATATCACG GGAGCAACGT GGCAGCCATC CGGAGGGTGC TAGACCGAGG AGAGCTGGGA
    GCAGGCACCG CCTCCATCCT GAGCTGCCGG CCCTTGAAAG GCAAGCCTGG GGTGGGGTTT
    GAGGAGCCTG GCGAGAACTG CGCCCCACCC CGGGAGGAGC AGCCCCCTTC CGTCTTGCTG
    TCCCCCTCCC TCCAGTATGC TGGGGCCGAG ACCCTAGCGT CCAAAGTGCA ATTCCGGGAT
    CCCAAGTCCC AGCGAACGCA CCAGGCCCAG GTGGCGTTCC AGGTGTGCGT GCGCCCGGGC
    TCCTACACCC CCGGACCCCC TTCGGCCACC CTCCGAGAAC CTCCCGACCC TCACTTCAGC
    CCCGCAGAAC TCGAGTGGAT AACCAAGGAG AAGGGGGCCA CACTCCTCTA TGCCCTGCTG
    GTGCGGGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288103
    Clone ID Related Accession (Same CDS sequence) XM_022418488.1
    Accession Version XM_022418488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGCAG GGGACACTGT GGGCGTGGTG
    CGGCGGGAGG ACGGGACCCT GCACTTCTTC GTCAACGGGA TGACCCAGGG CCCTGCGGCC
    TGGAATGTGC CCCCTGGCGT CTACGCTGTT GTGGACCTCT ATGGCCAGGC AGCCCAAGCT
    ACCATTGTGG ATGACGTGGA GGTGCCCCCG GCCCCTGAGC CCCTCTCTGA AGGGAACAAC
    CAGGTGTCTC CCAGCTCCCC ATCATCAGGG GCCGGGGGCT CTGACCTGCG CTTCCATCAG
    CTGCACGGCA GTAATGCAGT CATCACCAAC GGGGGCCGCA CCGCACTCCG GCACAACTGC
    CGCAGCGAGT TCAATGACGC CATCGTCATC TCCAACCGGG CCCTGCGGGA CGGAGAGCTG
    TTTGAAATCG TTATTCAGAA GATGGTGGAC CGCTGGTCAG GCTCCATTGA GGCTGGAGTG
    ACTGCCATTC GGCCGGAAGA CCTCGAATTC CCCAACACCA TGACAGACAT TGACTACGAC
    ACATGGATGC TGAGTGGCAC GGCCATCATG CAAGACGGGA ACACGATGCG CAACAACTAC
    GGGTGTGACC TGGACGCCCT GGGCACGGGT GCACGCATCG GCATGATGCG AACAGCCAAG
    GGTGACCTGC ACTACTTCAT CAACGGCCAG GACCAAGGCG CCGCCTGCTC AGGCTTGCCT
    CCGGGTAAAG AGGTGTACGC AGTAGTGGAT CTTTACGGCC AGTGTGTCCA AGTGTCCATC
    ACCAATGCCA CCGGCCCCAT GGACAACAGC TTGGCAACCA GCAACACTGC CACCGAGAAA
    TCCTTCCCTT TGCACTCCCC AGCGGCTGGC GTGGCTCACC GATTCCACAG TACTTGCGGC
    AAGAATGTGG CCCTGGAGGA GGATGGCACA AGGGCGGTAC GTGCGGTGGG CTATGCGCAT
    GGCCTGGTCT TCAGCACCAA GGAACTCAAG ACGGAGGAAG TATTTGAGGT GAAAGTAGAG
    GAACTGGATG AGAAGTGGGC GGGCTCCCTC CGGCTGGGGC TGACCACACT AGCGCCCGGG
    GAGATGGGGC CTGGCGCAGG TGGGGGCCCA GGGCTGCCTC CCTCCCTCCC GGAGCTCCGG
    ACAAAGACCA CCTGGATGGT GTCCAGCTGT GAAGTGAGGC GTGACGGGCA GCTCCAGAGG
    ATGAACTACG GCCGGAGCCT GGAGAGGCTG GGGGTGGGGA GCCGCGTGGG CATTCGTCGG
    GGCGCAGATG ACACGATGCA CATCCTGGTG GATGGAGAGG ACATGGGGCC CGCAGCTACC
    GGCATTGCCA AGAATGTGTG GGCCGTGCTG GATCTCTACG GGCCGGTACG GAGTGTGTCT
    GTGGTCAGCT CCACAAGGCT GGAGGAGCCC GAGGGCACCC AGCCTCCATC TCCCAGCTCG
    GACACGGGCA GCGAGGCCGA GGAAGATGAC GATGGGGAGG AGCACAGCTT GAGAGGTCAG
    AGTCAAGTGG CCATGATGTC GACGTCACTG GAGTTCCTGG AGAACCATGG GAAGAATATC
    CTTTTGTCCA ACGGGAACCG TACGGCCACA CGCGTAGCCA GCTACAACCA GGGCATCGTT
    GTCATCAACC AGCCCCTGGT GCCCCAGCTC CTAGTCCAGG TGCGGATAGA CTTCCTGAAC
    CGGCAGTGGA CATCTTCCCT GGTCCTGGGA GTCATCACCT GCCCGCCTGA GAGGCTCAAC
    TTCCCTGCTT CTGCCTGCGC CCTCAAACGG GCAGCCTGGC TGCTGCGGGG CCGGGGCATC
    TTCCACAATG GTCTCAAGAT TTGTGAGAAG TTTGGGCCAA ATCTGGACAC GTGTCCTGAA
    GGCACCATCC TGGGGCTGCG GCTAGACAGC TCTGGGGGCC TGCATCTCCA CGTCAATGGG
    ATGGACCAGG GGGTGGCGGT ATCAGATGTC CCCCAGCCCT GCCATGCGCT TGTGGACCTC
    TATGGGCAGT GTGAGCAGGT GACAATCGTG AGTCCTGAAC CAGGGGCAGC CAGTGGGAAG
    AGTGCTGGAA CCCAAGGGGA CATGGAGAAA GCGGACATGG TGGATGGTAT CAAGGAAAGT
    GTGTGCTGGG GCCCCCTGCC AGCTGCCAGC CCTCTCAAGA GCTGCGAGTA CCACGCCCTC
    TGCTCCCGCT TCCAAGAACT GCTGTTGCTT CCTGAGGACT ATTTCATGCC TCCACCCAAG
    CGGAGCCTGT GCTACTGTGA GTCTTGCCGG AAGCTTCGAG GAGGCTCCCA AGAGGGTGCG
    TGGAAGAAGC CACAGAACCT TTACTGGTCG AGGGGAGGAT CTTTGTCCAG TCTGCCCGGT
    CTACACCCTA GGGTGAATCC CCGCCTGGAA GCTGGAACGA TGACTAAGAA GTGGCACATG
    GCATATCACG GGAGCAACGT GGCAGCCATC CGGAGGGTGC TAGACCGAGG AGAGCTGGGA
    GCAGGCACCG CCTCCATCCT GAGCTGCCGG CCCTTGAAAG GCAAGCCTGG GGTGGGGTTT
    GAGGAGCCTG GCGAGAACTG CGCCCCACCC CGGGAGGAGC AGCCCCCTTC CGTCTTGCTG
    TCCCCCTCCC TCCAGTATGC TGGGGCCGAG ACCCTAGCGT CCAAAGTGCA ATTCCGGGAT
    CCCAAGTCCC AGCGAACGCA CCAGGCCCAG GTGGCGTTCC AGGTGTGCGT GCGCCCGGGC
    TCCTACACCC CCGGACCCCC TTCGGCCACC CTCCGAGAAC CTCCCGACCC TCACTTCAGC
    CCCGCAGAAC TCGAGTGGAT AACCAAGGAG AAGGGGGCCA CACTCCTCTA TGCCCTGCTG
    GTGCGGGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274196.1
    CDS145..4899
    Translation

    Target ORF information:

    RefSeq Version XM_022418488.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGGTGTG GGGAGAAGCC TGCTGCAGGG ACTCTAACAG GATCGGAGGA GGGGTGGAGA
    AGCAGGAGTG CCACAGATGA CTTCAACCAT GGCGTGGTGC TGAGTAGCAG AGCCCTGCGG
    GACGGAGAGG TGTTTCAGGT GCGCATCGAC AAGATGGTGG ACAAGTGGGC TGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCTGCC TACCTCCAGT TGCCCTCCAC CATGACCAAC
    TTGCGCTCTG GGACCTGGAT GATGACCGGG AATGGGGTGA TGCACAACGG AACGACCATT
    TTGGACGAGT ATGGGCACAA TCTGGACCGC CTCAAGGCAG GGGACACTGT GGGCGTGGTG
    CGGCGGGAGG ACGGGACCCT GCACTTCTTC GTCAACGGGA TGACCCAGGG CCCTGCGGCC
    TGGAATGTGC CCCCTGGCGT CTACGCTGTT GTGGACCTCT ATGGCCAGGC AGCCCAAGCT
    ACCATTGTGG ATGACGTGGA GGTGCCCCCG GCCCCTGAGC CCCTCTCTGA AGGGAACAAC
    CAGGTGTCTC CCAGCTCCCC ATCATCAGGG GCCGGGGGCT CTGACCTGCG CTTCCATCAG
    CTGCACGGCA GTAATGCAGT CATCACCAAC GGGGGCCGCA CCGCACTCCG GCACAACTGC
    CGCAGCGAGT TCAATGACGC CATCGTCATC TCCAACCGGG CCCTGCGGGA CGGAGAGCTG
    TTTGAAATCG TTATTCAGAA GATGGTGGAC CGCTGGTCAG GCTCCATTGA GGCTGGAGTG
    ACTGCCATTC GGCCGGAAGA CCTCGAATTC CCCAACACCA TGACAGACAT TGACTACGAC
    ACATGGATGC TGAGTGGCAC GGCCATCATG CAAGACGGGA ACACGATGCG CAACAACTAC
    GGGTGTGACC TGGACGCCCT GGGCACGGGT GCACGCATCG GCATGATGCG AACAGCCAAG
    GGTGACCTGC ACTACTTCAT CAACGGCCAG GACCAAGGCG CCGCCTGCTC AGGCTTGCCT
    CCGGGTAAAG AGGTGTACGC AGTAGTGGAT CTTTACGGCC AGTGTGTCCA AGTGTCCATC
    ACCAATGCCA CCGGCCCCAT GGACAACAGC TTGGCAACCA GCAACACTGC CACCGAGAAA
    TCCTTCCCTT TGCACTCCCC AGCGGCTGGC GTGGCTCACC GATTCCACAG TACTTGCGGC
    AAGAATGTGG CCCTGGAGGA GGATGGCACA AGGGCGGTAC GTGCGGTGGG CTATGCGCAT
    GGCCTGGTCT TCAGCACCAA GGAACTCAAG ACGGAGGAAG TATTTGAGGT GAAAGTAGAG
    GAACTGGATG AGAAGTGGGC GGGCTCCCTC CGGCTGGGGC TGACCACACT AGCGCCCGGG
    GAGATGGGGC CTGGCGCAGG TGGGGGCCCA GGGCTGCCTC CCTCCCTCCC GGAGCTCCGG
    ACAAAGACCA CCTGGATGGT GTCCAGCTGT GAAGTGAGGC GTGACGGGCA GCTCCAGAGG
    ATGAACTACG GCCGGAGCCT GGAGAGGCTG GGGGTGGGGA GCCGCGTGGG CATTCGTCGG
    GGCGCAGATG ACACGATGCA CATCCTGGTG GATGGAGAGG ACATGGGGCC CGCAGCTACC
    GGCATTGCCA AGAATGTGTG GGCCGTGCTG GATCTCTACG GGCCGGTACG GAGTGTGTCT
    GTGGTCAGCT CCACAAGGCT GGAGGAGCCC GAGGGCACCC AGCCTCCATC TCCCAGCTCG
    GACACGGGCA GCGAGGCCGA GGAAGATGAC GATGGGGAGG AGCACAGCTT GAGAGGTCAG
    AGTCAAGTGG CCATGATGTC GACGTCACTG GAGTTCCTGG AGAACCATGG GAAGAATATC
    CTTTTGTCCA ACGGGAACCG TACGGCCACA CGCGTAGCCA GCTACAACCA GGGCATCGTT
    GTCATCAACC AGCCCCTGGT GCCCCAGCTC CTAGTCCAGG TGCGGATAGA CTTCCTGAAC
    CGGCAGTGGA CATCTTCCCT GGTCCTGGGA GTCATCACCT GCCCGCCTGA GAGGCTCAAC
    TTCCCTGCTT CTGCCTGCGC CCTCAAACGG GCAGCCTGGC TGCTGCGGGG CCGGGGCATC
    TTCCACAATG GTCTCAAGAT TTGTGAGAAG TTTGGGCCAA ATCTGGACAC GTGTCCTGAA
    GGCACCATCC TGGGGCTGCG GCTAGACAGC TCTGGGGGCC TGCATCTCCA CGTCAATGGG
    ATGGACCAGG GGGTGGCGGT ATCAGATGTC CCCCAGCCCT GCCATGCGCT TGTGGACCTC
    TATGGGCAGT GTGAGCAGGT GACAATCGTG AGTCCTGAAC CAGGGGCAGC CAGTGGGAAG
    AGTGCTGGAA CCCAAGGGGA CATGGAGAAA GCGGACATGG TGGATGGTAT CAAGGAAAGT
    GTGTGCTGGG GCCCCCTGCC AGCTGCCAGC CCTCTCAAGA GCTGCGAGTA CCACGCCCTC
    TGCTCCCGCT TCCAAGAACT GCTGTTGCTT CCTGAGGACT ATTTCATGCC TCCACCCAAG
    CGGAGCCTGT GCTACTGTGA GTCTTGCCGG AAGCTTCGAG GAGGCTCCCA AGAGGGTGCG
    TGGAAGAAGC CACAGAACCT TTACTGGTCG AGGGGAGGAT CTTTGTCCAG TCTGCCCGGT
    CTACACCCTA GGGTGAATCC CCGCCTGGAA GCTGGAACGA TGACTAAGAA GTGGCACATG
    GCATATCACG GGAGCAACGT GGCAGCCATC CGGAGGGTGC TAGACCGAGG AGAGCTGGGA
    GCAGGCACCG CCTCCATCCT GAGCTGCCGG CCCTTGAAAG GCAAGCCTGG GGTGGGGTTT
    GAGGAGCCTG GCGAGAACTG CGCCCCACCC CGGGAGGAGC AGCCCCCTTC CGTCTTGCTG
    TCCCCCTCCC TCCAGTATGC TGGGGCCGAG ACCCTAGCGT CCAAAGTGCA ATTCCGGGAT
    CCCAAGTCCC AGCGAACGCA CCAGGCCCAG GTGGCGTTCC AGGTGTGCGT GCGCCCGGGC
    TCCTACACCC CCGGACCCCC TTCGGCCACC CTCCGAGAAC CTCCCGACCC TCACTTCAGC
    CCCGCAGAAC TCGAGTGGAT AACCAAGGAG AAGGGGGCCA CACTCCTCTA TGCCCTGCTG
    GTGCGGGTGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288104
    Clone ID Related Accession (Same CDS sequence) XM_022418489.1
    Accession Version XM_022418489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGTGGGGG AGCAGGCAGG GGACACTGTG
    GGCGTGGTGC GGCGGGAGGA CGGGACCCTG CACTTCTTCG TCAACGGGAT GACCCAGGGC
    CCTGCGGCCT GGAATGTGCC CCCTGGCGTC TACGCTGTTG TGGACCTCTA TGGCCAGGCA
    GCCCAAGCTA CCATTGTGGA TGACGTGGAG GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA
    GGGAACAACC AGGTGTCTCC CAGCTCCCCA TCATCAGGGG CCGGGGGCTC TGACCTGCGC
    TTCCATCAGC TGCACGGCAG TAATGCAGTC ATCACCAACG GGGGCCGCAC CGCACTCCGG
    CACAACTGCC GCAGCGAGTT CAATGACGCC ATCGTCATCT CCAACCGGGC CCTGCGGGAC
    GGAGAGCTGT TTGAAATCGT TATTCAGAAG ATGGTGGACC GCTGGTCAGG CTCCATTGAG
    GCTGGAGTGA CTGCCATTCG GCCGGAAGAC CTCGAATTCC CCAACACCAT GACAGACATT
    GACTACGACA CATGGATGCT GAGTGGCACG GCCATCATGC AAGACGGGAA CACGATGCGC
    AACAACTACG GGTGTGACCT GGACGCCCTG GGCACGGGTG CACGCATCGG CATGATGCGA
    ACAGCCAAGG GTGACCTGCA CTACTTCATC AACGGCCAGG ACCAAGGCGC CGCCTGCTCA
    GGCTTGCCTC CGGGTAAAGA GGTGTACGCA GTAGTGGATC TTTACGGCCA GTGTGTCCAA
    GTGTCCATCA CCAATGCCAC CGGCCCCATG GACAACAGCT TGGCAACCAG CAACACTGCC
    ACCGAGAAAT CCTTCCCTTT GCACTCCCCA GCGGCTGGCG TGGCTCACCG ATTCCACAGT
    ACTTGCGGCA AGAATGTGGC CCTGGAGGAG GATGGCACAA GGGCGGTACG TGCGGTGGGC
    TATGCGCATG GCCTGGTCTT CAGCACCAAG GAACTCAAGA CGGAGGAAGT ATTTGAGGTG
    AAAGTAGAGG AACTGGATGA GAAGTGGGCG GGCTCCCTCC GGCTGGGGCT GACCACACTA
    GCGCCCGGGG AGATGGGGCC TGGCGCAGGT GGGGGCCCAG GGCTGCCTCC CTCCCTCCCG
    GAGCTCCGGA CAAAGACCAC CTGGATGGTG TCCAGCTGTG AAGTGAGGCG TGACGGGCAG
    CTCCAGAGGA TGAACTACGG CCGGAGCCTG GAGAGGCTGG GGGTGGGGAG CCGCGTGGGC
    ATTCGTCGGG GCGCAGATGA CACGATGCAC ATCCTGGTGG ATGGAGAGGA CATGGGGCCC
    GCAGCTACCG GCATTGCCAA GAATGTGTGG GCCGTGCTGG ATCTCTACGG GCCGGTACGG
    AGTGTGTCTG TGGTCAGCTC CACAAGGCTG GAGGAGCCCG AGGGCACCCA GCCTCCATCT
    CCCAGCTCGG ACACGGGCAG CGAGGCCGAG GAAGATGACG ATGGGGAGGA GCACAGCTTG
    AGAGGTCAGA GTCAAGTGGC CATGATGTCG ACGTCACTGG AGTTCCTGGA GAACCATGGG
    AAGAATATCC TTTTGTCCAA CGGGAACCGT ACGGCCACAC GCGTAGCCAG CTACAACCAG
    GGCATCGTTG TCATCAACCA GCCCCTGGTG CCCCAGCTCC TAGTCCAGGT GCGGATAGAC
    TTCCTGAACC GGCAGTGGAC ATCTTCCCTG GTCCTGGGAG TCATCACCTG CCCGCCTGAG
    AGGCTCAACT TCCCTGCTTC TGCCTGCGCC CTCAAACGGG CAGCCTGGCT GCTGCGGGGC
    CGGGGCATCT TCCACAATGG TCTCAAGATT TGTGAGAAGT TTGGGCCAAA TCTGGACACG
    TGTCCTGAAG GCACCATCCT GGGGCTGCGG CTAGACAGCT CTGGGGGCCT GCATCTCCAC
    GTCAATGGGA TGGACCAGGG GGTGGCGGTA TCAGATGTCC CCCAGCCCTG CCATGCGCTT
    GTGGACCTCT ATGGGCAGTG TGAGCAGGTG ACAATCGTGA GTCCTGAACC AGGGGCAGCC
    AGTGGGAAGA GTGCTGGAAC CCAAGGGGAC ATGGAGAAAG CGGACATGGT GGATGGTATC
    AAGGAAAGTG TGTGCTGGGG CCCCCTGCCA GCTGCCAGCC CTCTCAAGAG CTGCGAGTAC
    CACGCCCTCT GCTCCCGCTT CCAAGAACTG CTGTTGCTTC CTGAGGACTA TTTCATGCCT
    CCACCCAAGC GGAGCCTGTG CTACTGTGAG TCTTGCCGGA AGCTTCGAGG AGGCTCCCAA
    GAGGGTGCGT GGAAGAAGCC ACAGAACCTT TACTGGTCGA GGGGAGGATC TTTGTCCAGT
    CTGCCCGGTC TACACCCTAG GGTGAATCCC CGCCTGGAAG CTGGAACGAT GACTAAGAAG
    TGGCACATGG CATATCACGG GAGCAACGTG GCAGCCATCC GGAGGGTGCT AGACCGAGGA
    GAGCTGGGAG CAGGCACCGC CTCCATCCTG AGCTGCCGGC CCTTGAAAGG CAAGCCTGGG
    GTGGGGTTTG AGGAGCCTGG CGAGAACTGC GCCCCACCCC GGGAGGAGCA GCCCCCTTCC
    GTCTTGCTGT CCCCCTCCCT CCAGTATGCT GGGGCCGAGA CCCTAGCGTC CAAAGTGCAA
    TTCCGGGATC CCAAGTCCCA GCGAACGCAC CAGGCCCAGG TGGCGTTCCA GGTGTGCGTG
    CGCCCGGGCT CCTACACCCC CGGACCCCCT TCGGCCACCC TCCGAGAACC TCCCGACCCT
    CACTTCAGCC CCGCAGAACT CGAGTGGATA ACCAAGGAGA AGGGGGCCAC ACTCCTCTAT
    GCCCTGCTGG TGCGGGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274197.1
    CDS145..4848
    Translation

    Target ORF information:

    RefSeq Version XM_022418489.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGTGGGGG AGCAGGCAGG GGACACTGTG
    GGCGTGGTGC GGCGGGAGGA CGGGACCCTG CACTTCTTCG TCAACGGGAT GACCCAGGGC
    CCTGCGGCCT GGAATGTGCC CCCTGGCGTC TACGCTGTTG TGGACCTCTA TGGCCAGGCA
    GCCCAAGCTA CCATTGTGGA TGACGTGGAG GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA
    GGGAACAACC AGGTGTCTCC CAGCTCCCCA TCATCAGGGG CCGGGGGCTC TGACCTGCGC
    TTCCATCAGC TGCACGGCAG TAATGCAGTC ATCACCAACG GGGGCCGCAC CGCACTCCGG
    CACAACTGCC GCAGCGAGTT CAATGACGCC ATCGTCATCT CCAACCGGGC CCTGCGGGAC
    GGAGAGCTGT TTGAAATCGT TATTCAGAAG ATGGTGGACC GCTGGTCAGG CTCCATTGAG
    GCTGGAGTGA CTGCCATTCG GCCGGAAGAC CTCGAATTCC CCAACACCAT GACAGACATT
    GACTACGACA CATGGATGCT GAGTGGCACG GCCATCATGC AAGACGGGAA CACGATGCGC
    AACAACTACG GGTGTGACCT GGACGCCCTG GGCACGGGTG CACGCATCGG CATGATGCGA
    ACAGCCAAGG GTGACCTGCA CTACTTCATC AACGGCCAGG ACCAAGGCGC CGCCTGCTCA
    GGCTTGCCTC CGGGTAAAGA GGTGTACGCA GTAGTGGATC TTTACGGCCA GTGTGTCCAA
    GTGTCCATCA CCAATGCCAC CGGCCCCATG GACAACAGCT TGGCAACCAG CAACACTGCC
    ACCGAGAAAT CCTTCCCTTT GCACTCCCCA GCGGCTGGCG TGGCTCACCG ATTCCACAGT
    ACTTGCGGCA AGAATGTGGC CCTGGAGGAG GATGGCACAA GGGCGGTACG TGCGGTGGGC
    TATGCGCATG GCCTGGTCTT CAGCACCAAG GAACTCAAGA CGGAGGAAGT ATTTGAGGTG
    AAAGTAGAGG AACTGGATGA GAAGTGGGCG GGCTCCCTCC GGCTGGGGCT GACCACACTA
    GCGCCCGGGG AGATGGGGCC TGGCGCAGGT GGGGGCCCAG GGCTGCCTCC CTCCCTCCCG
    GAGCTCCGGA CAAAGACCAC CTGGATGGTG TCCAGCTGTG AAGTGAGGCG TGACGGGCAG
    CTCCAGAGGA TGAACTACGG CCGGAGCCTG GAGAGGCTGG GGGTGGGGAG CCGCGTGGGC
    ATTCGTCGGG GCGCAGATGA CACGATGCAC ATCCTGGTGG ATGGAGAGGA CATGGGGCCC
    GCAGCTACCG GCATTGCCAA GAATGTGTGG GCCGTGCTGG ATCTCTACGG GCCGGTACGG
    AGTGTGTCTG TGGTCAGCTC CACAAGGCTG GAGGAGCCCG AGGGCACCCA GCCTCCATCT
    CCCAGCTCGG ACACGGGCAG CGAGGCCGAG GAAGATGACG ATGGGGAGGA GCACAGCTTG
    AGAGGTCAGA GTCAAGTGGC CATGATGTCG ACGTCACTGG AGTTCCTGGA GAACCATGGG
    AAGAATATCC TTTTGTCCAA CGGGAACCGT ACGGCCACAC GCGTAGCCAG CTACAACCAG
    GGCATCGTTG TCATCAACCA GCCCCTGGTG CCCCAGCTCC TAGTCCAGGT GCGGATAGAC
    TTCCTGAACC GGCAGTGGAC ATCTTCCCTG GTCCTGGGAG TCATCACCTG CCCGCCTGAG
    AGGCTCAACT TCCCTGCTTC TGCCTGCGCC CTCAAACGGG CAGCCTGGCT GCTGCGGGGC
    CGGGGCATCT TCCACAATGG TCTCAAGATT TGTGAGAAGT TTGGGCCAAA TCTGGACACG
    TGTCCTGAAG GCACCATCCT GGGGCTGCGG CTAGACAGCT CTGGGGGCCT GCATCTCCAC
    GTCAATGGGA TGGACCAGGG GGTGGCGGTA TCAGATGTCC CCCAGCCCTG CCATGCGCTT
    GTGGACCTCT ATGGGCAGTG TGAGCAGGTG ACAATCGTGA GTCCTGAACC AGGGGCAGCC
    AGTGGGAAGA GTGCTGGAAC CCAAGGGGAC ATGGAGAAAG CGGACATGGT GGATGGTATC
    AAGGAAAGTG TGTGCTGGGG CCCCCTGCCA GCTGCCAGCC CTCTCAAGAG CTGCGAGTAC
    CACGCCCTCT GCTCCCGCTT CCAAGAACTG CTGTTGCTTC CTGAGGACTA TTTCATGCCT
    CCACCCAAGC GGAGCCTGTG CTACTGTGAG TCTTGCCGGA AGCTTCGAGG AGGCTCCCAA
    GAGGGTGCGT GGAAGAAGCC ACAGAACCTT TACTGGTCGA GGGGAGGATC TTTGTCCAGT
    CTGCCCGGTC TACACCCTAG GGTGAATCCC CGCCTGGAAG CTGGAACGAT GACTAAGAAG
    TGGCACATGG CATATCACGG GAGCAACGTG GCAGCCATCC GGAGGGTGCT AGACCGAGGA
    GAGCTGGGAG CAGGCACCGC CTCCATCCTG AGCTGCCGGC CCTTGAAAGG CAAGCCTGGG
    GTGGGGTTTG AGGAGCCTGG CGAGAACTGC GCCCCACCCC GGGAGGAGCA GCCCCCTTCC
    GTCTTGCTGT CCCCCTCCCT CCAGTATGCT GGGGCCGAGA CCCTAGCGTC CAAAGTGCAA
    TTCCGGGATC CCAAGTCCCA GCGAACGCAC CAGGCCCAGG TGGCGTTCCA GGTGTGCGTG
    CGCCCGGGCT CCTACACCCC CGGACCCCCT TCGGCCACCC TCCGAGAACC TCCCGACCCT
    CACTTCAGCC CCGCAGAACT CGAGTGGATA ACCAAGGAGA AGGGGGCCAC ACTCCTCTAT
    GCCCTGCTGG TGCGGGTGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288105
    Clone ID Related Accession (Same CDS sequence) XM_022418490.1
    Accession Version XM_022418490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGA CTTTGCCAGC
    ATGGAGCTCT CTGAGGTGGT GGGCAACGCC ATCCTTTCTG CCTACAACGG GGGGCTCCTA
    AATGTGAACC TGAGCTCCCC ACCGGCAGGG GAAGGACTGG GGTCTGGCGG GGCTGCCACC
    TCGCCCATCC TCACCTCCAA CGACGCCCTG CTCTTTCACG AGAAGTGTGG GACTCTCATC
    AAACTCAGCA ACAACAATAA GACAGCTGAG CGCCGCCGGC CCCTGGACGA ATTCAACAAC
    GGGGTTGTCA TGACCAACCG CCCTCTCCGG GACAATGAGA TGTTTGAGAT CCGCATTGAC
    AAGCTCGTAG ACAAGTGGTC GGGCTCCATT GAGATTGGTG TCACCACCCA CAACCCCAAC
    AACCTGGAGT ACCCTGCCAC CATGACCAAT TTACAGTCAG GCACCATCAT GATGAGCGGC
    TGTGGGATCC TGACCAATGG CAAGGGCACC CGCCGGGAGT ACTGTGAATT CAGCCTGGAT
    GAGCTGCAGG AGGGCGACCA CATTGGTCTC ACAAGGAAGT CCAACTCAGC CCTGCACTTC
    TTTATCAATG GCATTGATCA GGGCGTGGCT ACCCCACTGA CACCCCCAGT GGTGTACGGC
    GTGGTGGACT TGTACGGGAT GGCTGTGAAG GTGACCATCG TCCACAATAA CAACCACAGC
    GACCGGCTGC GTCGAAACAA TGCCATCCTG CGGGCACTGT CCCCCGAGGG CGCCCTGCGT
    CGCGCCACTC CTGCCACCCA GGCAGAGCCC GAGCGCCTGC TCTTCCACCC TAACTGCGGG
    CAGAAGGCAG CTATCACCCA CGAGGGACGC ACTGCCCTGA GGCCCCAGTA TGGCCCATGG
    TGTGGGGAGA AGCCTGCTGC AGGGACTCTA ACAGGATCGG AGGAGGGGTG GAGAAGCAGG
    AGTGCCACAG ATGACTTCAA CCATGGCGTG GTGCTGAGTA GCAGAGCCCT GCGGGACGGA
    GAGGTGTTTC AGGTGCGCAT CGACAAGATG GTGGACAAGT GGGCTGGCTC CATTGAGATT
    GGTGTCACCA CCCACAACCC TGCCTACCTC CAGTTGCCCT CCACCATGAC CAACTTGCGC
    TCTGGGACCT GGATGATGAC CGGGAATGGG GTGATGCACA ACGGAACGAC CATTTTGGAC
    GAGTATGGGC ACAATCTGGA CCGCCTCAAG GTGGGGGAGC AGGCAGGGGA CACTGTGGGC
    GTGGTGCGGC GGGAGGACGG GACCCTGCAC TTCTTCGTCA ACGGGATGAC CCAGGGCCCT
    GCGGCCTGGA ATGTGCCCCC TGGCGTCTAC GCTGTTGTGG ACCTCTATGG CCAGGCAGCC
    CAAGCTACCA TTGTGGATGA CGTGGAGGTG CCCCCGGCCC CTGAGCCCCT CTCTGAAGGG
    AACAACCAGG TGTCTCCCAG CTCCCCATCA TCAGGGGCCG GGGGCTCTGA CCTGCGCTTC
    CATCAGCTGC ACGGCAGTAA TGCAGTCATC ACCAACGGGG GCCGCACCGC ACTCCGGCAC
    AACTGCCGCA GCGAGTTCAA TGACGCCATC GTCATCTCCA ACCGGGCCCT GCGGGACGGA
    GAGCTGTTTG AAATCGTTAT TCAGAAGATG GTGGACCGCT GGTCAGGCTC CATTGAGGCT
    GGAGTGACTG CCATTCGGCC GGAAGACCTC GAATTCCCCA ACACCATGAC AGACATTGAC
    TACGACACAT GGATGCTGAG TGGCACGGCC ATCATGCAAG ACGGGAACAC GATGCGCAAC
    AACTACGGGT GTGACCTGGA CGCCCTGGGC ACGGGTGCAC GCATCGGCAT GATGCGAACA
    GCCAAGGGTG ACCTGCACTA CTTCATCAAC GGCCAGGACC AAGGCGCCGC CTGCTCAGGC
    TTGCCTCCGG GTAAAGAGGT GTACGCAGTA GTGGATCTTT ACGGCCAGTG TGTCCAAGTG
    TCCATCACCA ATGCCACCGG CCCCATGGAC AACAGCTTGG CAACCAGCAA CACTGCCACC
    GAGAAATCCT TCCCTTTGCA CTCCCCAGCG GCTGGCGTGG CTCACCGATT CCACAGTACT
    TGCGGCAAGA ATGTGGCCCT GGAGGAGGAT GGCACAAGGG CGGTACGTGC GGTGGGCTAT
    GCGCATGGCC TGGTCTTCAG CACCAAGGAA CTCAAGACGG AGGAAGTATT TGAGGTGAAA
    GTAGAGGAAC TGGATGAGAA GTGGGCGGGC TCCCTCCGGC TGGGGCTGAC CACACTAGCG
    CCCGGGGAGA TGGGGCCTGG CGCAGGTGGG GGCCCAGGGC TGCCTCCCTC CCTCCCGGAG
    CTCCGGACAA AGACCACCTG GATGGTGTCC AGCTGTGAAG TGAGGCGTGA CGGGCAGCTC
    CAGAGGATGA ACTACGGCCG GAGCCTGGAG AGGCTGGGGG TGGGGAGCCG CGTGGGCATT
    CGTCGGGGCG CAGATGACAC GATGCACATC CTGGTGGATG GAGAGGACAT GGGGCCCGCA
    GCTACCGGCA TTGCCAAGAA TGTGTGGGCC GTGCTGGATC TCTACGGGCC GGTACGGAGT
    GTGTCTGTGG TCAGCTCCAC AAGGCTGGAG GAGCCCGAGG GCACCCAGCC TCCATCTCCC
    AGCTCGGACA CGGGCAGCGA GGCCGAGGAA GATGACGATG GGGAGGAGCA CAGCTTGAGA
    GGTCAGAGTC AAGTGGCCAT GATGTCGACG TCACTGGAGT TCCTGGAGAA CCATGGGAAG
    AATATCCTTT TGTCCAACGG GAACCGTACG GCCACACGCG TAGCCAGCTA CAACCAGGGC
    ATCGTTGTCA TCAACCAGCC CCTGGTGCCC CAGCTCCTAG TCCAGGTGCG GATAGACTTC
    CTGAACCGGC AGTGGACATC TTCCCTGGTC CTGGGAGTCA TCACCTGCCC GCCTGAGAGG
    CTCAACTTCC CTGCTTCTGC CTGCGCCCTC AAACGGGCAG CCTGGCTGCT GCGGGGCCGG
    GGCATCTTCC ACAATGGTCT CAAGATTTGT GAGAAGTTTG GGCCAAATCT GGACACGTGT
    CCTGAAGGCA CCATCCTGGG GCTGCGGCTA GACAGCTCTG GGGGCCTGCA TCTCCACGTC
    AATGGGATGG ACCAGGGGGT GGCGGTATCA GATGTCCCCC AGCCCTGCCA TGCGCTTGTG
    GACCTCTATG GGCAGTGTGA GCAGGTGACA ATCGTGAGTC CTGAACCAGG GGCAGCCAGT
    GGGAAGAGTG CTGGAACCCA AGGGGACATG GAGAAAGCGG ACATGGTGGA TGGTATCAAG
    GAAAGTGTGT GCTGGGGCCC CCTGCCAGCT GCCAGCCCTC TCAAGAGCTG CGAGTACCAC
    GCCCTCTGCT CCCGCTTCCA AGAACTGCTG TTGCTTCCTG AGGACTATTT CATGCCTCCA
    CCCAAGCGGA GCCTGTGCTA CTGTGAGTCT TGCCGGAAGC TTCGAGGAGG CTCCCAAGAG
    GGTGCGTGGA AGAAGCCACA GAACCTTTAC TGGTCGAGGG GAGGATCTTT GTCCAGTCTG
    CCCGGTCTAC ACCCTAGGGT GAATCCCCGC CTGGAAGCTG GAACGATGAC TAAGAAGTGG
    CACATGGCAT ATCACGGGAG CAACGTGGCA GCCATCCGGA GGGTGCTAGA CCGAGGAGAG
    CTGGGAGCAG GCACCGCCTC CATCCTGAGC TGCCGGCCCT TGAAAGGCAA GCCTGGGGTG
    GGGTTTGAGG AGCCTGGCGA GAACTGCGCC CCACCCCGGG AGGAGCAGCC CCCTTCCGTC
    TTGCTGTCCC CCTCCCTCCA GTATGCTGGG GCCGAGACCC TAGCGTCCAA AGTGCAATTC
    CGGGATCCCA AGTCCCAGCG AACGCACCAG GCCCAGGTGG CGTTCCAGGT GTGCGTGCGC
    CCGGGCTCCT ACACCCCCGG ACCCCCTTCG GCCACCCTCC GAGAACCTCC CGACCCTCAC
    TTCAGCCCCG CAGAACTCGA GTGGATAACC AAGGAGAAGG GGGCCACACT CCTCTATGCC
    CTGCTGGTGC GGGTGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274198.1
    CDS145..4845
    Translation

    Target ORF information:

    RefSeq Version XM_022418490.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGA CTTTGCCAGC
    ATGGAGCTCT CTGAGGTGGT GGGCAACGCC ATCCTTTCTG CCTACAACGG GGGGCTCCTA
    AATGTGAACC TGAGCTCCCC ACCGGCAGGG GAAGGACTGG GGTCTGGCGG GGCTGCCACC
    TCGCCCATCC TCACCTCCAA CGACGCCCTG CTCTTTCACG AGAAGTGTGG GACTCTCATC
    AAACTCAGCA ACAACAATAA GACAGCTGAG CGCCGCCGGC CCCTGGACGA ATTCAACAAC
    GGGGTTGTCA TGACCAACCG CCCTCTCCGG GACAATGAGA TGTTTGAGAT CCGCATTGAC
    AAGCTCGTAG ACAAGTGGTC GGGCTCCATT GAGATTGGTG TCACCACCCA CAACCCCAAC
    AACCTGGAGT ACCCTGCCAC CATGACCAAT TTACAGTCAG GCACCATCAT GATGAGCGGC
    TGTGGGATCC TGACCAATGG CAAGGGCACC CGCCGGGAGT ACTGTGAATT CAGCCTGGAT
    GAGCTGCAGG AGGGCGACCA CATTGGTCTC ACAAGGAAGT CCAACTCAGC CCTGCACTTC
    TTTATCAATG GCATTGATCA GGGCGTGGCT ACCCCACTGA CACCCCCAGT GGTGTACGGC
    GTGGTGGACT TGTACGGGAT GGCTGTGAAG GTGACCATCG TCCACAATAA CAACCACAGC
    GACCGGCTGC GTCGAAACAA TGCCATCCTG CGGGCACTGT CCCCCGAGGG CGCCCTGCGT
    CGCGCCACTC CTGCCACCCA GGCAGAGCCC GAGCGCCTGC TCTTCCACCC TAACTGCGGG
    CAGAAGGCAG CTATCACCCA CGAGGGACGC ACTGCCCTGA GGCCCCAGTA TGGCCCATGG
    TGTGGGGAGA AGCCTGCTGC AGGGACTCTA ACAGGATCGG AGGAGGGGTG GAGAAGCAGG
    AGTGCCACAG ATGACTTCAA CCATGGCGTG GTGCTGAGTA GCAGAGCCCT GCGGGACGGA
    GAGGTGTTTC AGGTGCGCAT CGACAAGATG GTGGACAAGT GGGCTGGCTC CATTGAGATT
    GGTGTCACCA CCCACAACCC TGCCTACCTC CAGTTGCCCT CCACCATGAC CAACTTGCGC
    TCTGGGACCT GGATGATGAC CGGGAATGGG GTGATGCACA ACGGAACGAC CATTTTGGAC
    GAGTATGGGC ACAATCTGGA CCGCCTCAAG GTGGGGGAGC AGGCAGGGGA CACTGTGGGC
    GTGGTGCGGC GGGAGGACGG GACCCTGCAC TTCTTCGTCA ACGGGATGAC CCAGGGCCCT
    GCGGCCTGGA ATGTGCCCCC TGGCGTCTAC GCTGTTGTGG ACCTCTATGG CCAGGCAGCC
    CAAGCTACCA TTGTGGATGA CGTGGAGGTG CCCCCGGCCC CTGAGCCCCT CTCTGAAGGG
    AACAACCAGG TGTCTCCCAG CTCCCCATCA TCAGGGGCCG GGGGCTCTGA CCTGCGCTTC
    CATCAGCTGC ACGGCAGTAA TGCAGTCATC ACCAACGGGG GCCGCACCGC ACTCCGGCAC
    AACTGCCGCA GCGAGTTCAA TGACGCCATC GTCATCTCCA ACCGGGCCCT GCGGGACGGA
    GAGCTGTTTG AAATCGTTAT TCAGAAGATG GTGGACCGCT GGTCAGGCTC CATTGAGGCT
    GGAGTGACTG CCATTCGGCC GGAAGACCTC GAATTCCCCA ACACCATGAC AGACATTGAC
    TACGACACAT GGATGCTGAG TGGCACGGCC ATCATGCAAG ACGGGAACAC GATGCGCAAC
    AACTACGGGT GTGACCTGGA CGCCCTGGGC ACGGGTGCAC GCATCGGCAT GATGCGAACA
    GCCAAGGGTG ACCTGCACTA CTTCATCAAC GGCCAGGACC AAGGCGCCGC CTGCTCAGGC
    TTGCCTCCGG GTAAAGAGGT GTACGCAGTA GTGGATCTTT ACGGCCAGTG TGTCCAAGTG
    TCCATCACCA ATGCCACCGG CCCCATGGAC AACAGCTTGG CAACCAGCAA CACTGCCACC
    GAGAAATCCT TCCCTTTGCA CTCCCCAGCG GCTGGCGTGG CTCACCGATT CCACAGTACT
    TGCGGCAAGA ATGTGGCCCT GGAGGAGGAT GGCACAAGGG CGGTACGTGC GGTGGGCTAT
    GCGCATGGCC TGGTCTTCAG CACCAAGGAA CTCAAGACGG AGGAAGTATT TGAGGTGAAA
    GTAGAGGAAC TGGATGAGAA GTGGGCGGGC TCCCTCCGGC TGGGGCTGAC CACACTAGCG
    CCCGGGGAGA TGGGGCCTGG CGCAGGTGGG GGCCCAGGGC TGCCTCCCTC CCTCCCGGAG
    CTCCGGACAA AGACCACCTG GATGGTGTCC AGCTGTGAAG TGAGGCGTGA CGGGCAGCTC
    CAGAGGATGA ACTACGGCCG GAGCCTGGAG AGGCTGGGGG TGGGGAGCCG CGTGGGCATT
    CGTCGGGGCG CAGATGACAC GATGCACATC CTGGTGGATG GAGAGGACAT GGGGCCCGCA
    GCTACCGGCA TTGCCAAGAA TGTGTGGGCC GTGCTGGATC TCTACGGGCC GGTACGGAGT
    GTGTCTGTGG TCAGCTCCAC AAGGCTGGAG GAGCCCGAGG GCACCCAGCC TCCATCTCCC
    AGCTCGGACA CGGGCAGCGA GGCCGAGGAA GATGACGATG GGGAGGAGCA CAGCTTGAGA
    GGTCAGAGTC AAGTGGCCAT GATGTCGACG TCACTGGAGT TCCTGGAGAA CCATGGGAAG
    AATATCCTTT TGTCCAACGG GAACCGTACG GCCACACGCG TAGCCAGCTA CAACCAGGGC
    ATCGTTGTCA TCAACCAGCC CCTGGTGCCC CAGCTCCTAG TCCAGGTGCG GATAGACTTC
    CTGAACCGGC AGTGGACATC TTCCCTGGTC CTGGGAGTCA TCACCTGCCC GCCTGAGAGG
    CTCAACTTCC CTGCTTCTGC CTGCGCCCTC AAACGGGCAG CCTGGCTGCT GCGGGGCCGG
    GGCATCTTCC ACAATGGTCT CAAGATTTGT GAGAAGTTTG GGCCAAATCT GGACACGTGT
    CCTGAAGGCA CCATCCTGGG GCTGCGGCTA GACAGCTCTG GGGGCCTGCA TCTCCACGTC
    AATGGGATGG ACCAGGGGGT GGCGGTATCA GATGTCCCCC AGCCCTGCCA TGCGCTTGTG
    GACCTCTATG GGCAGTGTGA GCAGGTGACA ATCGTGAGTC CTGAACCAGG GGCAGCCAGT
    GGGAAGAGTG CTGGAACCCA AGGGGACATG GAGAAAGCGG ACATGGTGGA TGGTATCAAG
    GAAAGTGTGT GCTGGGGCCC CCTGCCAGCT GCCAGCCCTC TCAAGAGCTG CGAGTACCAC
    GCCCTCTGCT CCCGCTTCCA AGAACTGCTG TTGCTTCCTG AGGACTATTT CATGCCTCCA
    CCCAAGCGGA GCCTGTGCTA CTGTGAGTCT TGCCGGAAGC TTCGAGGAGG CTCCCAAGAG
    GGTGCGTGGA AGAAGCCACA GAACCTTTAC TGGTCGAGGG GAGGATCTTT GTCCAGTCTG
    CCCGGTCTAC ACCCTAGGGT GAATCCCCGC CTGGAAGCTG GAACGATGAC TAAGAAGTGG
    CACATGGCAT ATCACGGGAG CAACGTGGCA GCCATCCGGA GGGTGCTAGA CCGAGGAGAG
    CTGGGAGCAG GCACCGCCTC CATCCTGAGC TGCCGGCCCT TGAAAGGCAA GCCTGGGGTG
    GGGTTTGAGG AGCCTGGCGA GAACTGCGCC CCACCCCGGG AGGAGCAGCC CCCTTCCGTC
    TTGCTGTCCC CCTCCCTCCA GTATGCTGGG GCCGAGACCC TAGCGTCCAA AGTGCAATTC
    CGGGATCCCA AGTCCCAGCG AACGCACCAG GCCCAGGTGG CGTTCCAGGT GTGCGTGCGC
    CCGGGCTCCT ACACCCCCGG ACCCCCTTCG GCCACCCTCC GAGAACCTCC CGACCCTCAC
    TTCAGCCCCG CAGAACTCGA GTGGATAACC AAGGAGAAGG GGGCCACACT CCTCTATGCC
    CTGCTGGTGC GGGTGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288106
    Clone ID Related Accession (Same CDS sequence) XM_022418491.1
    Accession Version XM_022418491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGTGGGGGAG CAGGCAGGGG ACACTGTGGG CGTGGTGCGG
    CGGGAGGACG GGACCCTGCA CTTCTTCGTC AACGGGATGA CCCAGGGCCC TGCGGCCTGG
    AATGTGCCCC CTGGCGTCTA CGCTGTTGTG GACCTCTATG GCCAGGCAGC CCAAGCTACC
    ATTGTGGATG ACGTGGAGGT GCCCCCGGCC CCTGAGCCCC TCTCTGAAGG GAACAACCAG
    GTGTCTCCCA GCTCCCCATC ATCAGGGGCC GGGGGCTCTG ACCTGCGCTT CCATCAGCTG
    CACGGCAGTA ATGCAGTCAT CACCAACGGG GGCCGCACCG CACTCCGGCA CAACTGCCGC
    AGCGAGTTCA ATGACGCCAT CGTCATCTCC AACCGGGCCC TGCGGGACGG AGAGCTGTTT
    GAAATCGTTA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACT
    GCCATTCGGC CGGAAGACCT CGAATTCCCC AACACCATGA CAGACATTGA CTACGACACA
    TGGATGCTGA GTGGCACGGC CATCATGCAA GACGGGAACA CGATGCGCAA CAACTACGGG
    TGTGACCTGG ACGCCCTGGG CACGGGTGCA CGCATCGGCA TGATGCGAAC AGCCAAGGGT
    GACCTGCACT ACTTCATCAA CGGCCAGGAC CAAGGCGCCG CCTGCTCAGG CTTGCCTCCG
    GGTAAAGAGG TGTACGCAGT AGTGGATCTT TACGGCCAGT GTGTCCAAGT GTCCATCACC
    AATGCCACCG GCCCCATGGA CAACAGCTTG GCAACCAGCA ACACTGCCAC CGAGAAATCC
    TTCCCTTTGC ACTCCCCAGC GGCTGGCGTG GCTCACCGAT TCCACAGTAC TTGCGGCAAG
    AATGTGGCCC TGGAGGAGGA TGGCACAAGG GCGGTACGTG CGGTGGGCTA TGCGCATGGC
    CTGGTCTTCA GCACCAAGGA ACTCAAGACG GAGGAAGTAT TTGAGGTGAA AGTAGAGGAA
    CTGGATGAGA AGTGGGCGGG CTCCCTCCGG CTGGGGCTGA CCACACTAGC GCCCGGGGAG
    ATGGGGCCTG GCGCAGGTGG GGGCCCAGGG CTGCCTCCCT CCCTCCCGGA GCTCCGGACA
    AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGTG ACGGGCAGCT CCAGAGGATG
    AACTACGGCC GGAGCCTGGA GAGGCTGGGG GTGGGGAGCC GCGTGGGCAT TCGTCGGGGC
    GCAGATGACA CGATGCACAT CCTGGTGGAT GGAGAGGACA TGGGGCCCGC AGCTACCGGC
    ATTGCCAAGA ATGTGTGGGC CGTGCTGGAT CTCTACGGGC CGGTACGGAG TGTGTCTGTG
    GTCAGCTCCA CAAGGCTGGA GGAGCCCGAG GGCACCCAGC CTCCATCTCC CAGCTCGGAC
    ACGGGCAGCG AGGCCGAGGA AGATGACGAT GGGGAGGAGC ACAGCTTGAG AGGTCAGAGT
    CAAGTGGCCA TGATGTCGAC GTCACTGGAG TTCCTGGAGA ACCATGGGAA GAATATCCTT
    TTGTCCAACG GGAACCGTAC GGCCACACGC GTAGCCAGCT ACAACCAGGG CATCGTTGTC
    ATCAACCAGC CCCTGGTGCC CCAGCTCCTA GTCCAGGTGC GGATAGACTT CCTGAACCGG
    CAGTGGACAT CTTCCCTGGT CCTGGGAGTC ATCACCTGCC CGCCTGAGAG GCTCAACTTC
    CCTGCTTCTG CCTGCGCCCT CAAACGGGCA GCCTGGCTGC TGCGGGGCCG GGGCATCTTC
    CACAATGGTC TCAAGATTTG TGAGAAGTTT GGGCCAAATC TGGACACGTG TCCTGAAGGC
    ACCATCCTGG GGCTGCGGCT AGACAGCTCT GGGGGCCTGC ATCTCCACGT CAATGGGATG
    GACCAGGGGG TGGCGGTATC AGATGTCCCC CAGCCCTGCC ATGCGCTTGT GGACCTCTAT
    GGGCAGTGTG AGCAGGTGAC AATCGTGAGT CCTGAACCAG GGGCAGCCAG TGGGAAGAGT
    GCTGGAACCC AAGGGGACAT GGAGAAAGCG GACATGGTGG ATGGTATCAA GGAAAGTGTG
    TGCTGGGGCC CCCTGCCAGC TGCCAGCCCT CTCAAGAGCT GCGAGTACCA CGCCCTCTGC
    TCCCGCTTCC AAGAACTGCT GTTGCTTCCT GAGGACTATT TCATGCCTCC ACCCAAGCGG
    AGCCTGTGCT ACTGTGAGTC TTGCCGGAAG CTTCGAGGAG GCTCCCAAGA GGGTGCGTGG
    AAGAAGCCAC AGAACCTTTA CTGGTCGAGG GGAGGATCTT TGTCCAGTCT GCCCGGTCTA
    CACCCTAGGG TGAATCCCCG CCTGGAAGCT GGAACGATGA CTAAGAAGTG GCACATGGCA
    TATCACGGGA GCAACGTGGC AGCCATCCGG AGGGTGCTAG ACCGAGGAGA GCTGGGAGCA
    GGCACCGCCT CCATCCTGAG CTGCCGGCCC TTGAAAGGCA AGCCTGGGGT GGGGTTTGAG
    GAGCCTGGCG AGAACTGCGC CCCACCCCGG GAGGAGCAGC CCCCTTCCGT CTTGCTGTCC
    CCCTCCCTCC AGTATGCTGG GGCCGAGACC CTAGCGTCCA AAGTGCAATT CCGGGATCCC
    AAGTCCCAGC GAACGCACCA GGCCCAGGTG GCGTTCCAGG TGTGCGTGCG CCCGGGCTCC
    TACACCCCCG GACCCCCTTC GGCCACCCTC CGAGAACCTC CCGACCCTCA CTTCAGCCCC
    GCAGAACTCG AGTGGATAAC CAAGGAGAAG GGGGCCACAC TCCTCTATGC CCTGCTGGTG
    CGGGTGGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274199.1
    CDS145..4836
    Translation

    Target ORF information:

    RefSeq Version XM_022418491.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGTGGGGGAG CAGGCAGGGG ACACTGTGGG CGTGGTGCGG
    CGGGAGGACG GGACCCTGCA CTTCTTCGTC AACGGGATGA CCCAGGGCCC TGCGGCCTGG
    AATGTGCCCC CTGGCGTCTA CGCTGTTGTG GACCTCTATG GCCAGGCAGC CCAAGCTACC
    ATTGTGGATG ACGTGGAGGT GCCCCCGGCC CCTGAGCCCC TCTCTGAAGG GAACAACCAG
    GTGTCTCCCA GCTCCCCATC ATCAGGGGCC GGGGGCTCTG ACCTGCGCTT CCATCAGCTG
    CACGGCAGTA ATGCAGTCAT CACCAACGGG GGCCGCACCG CACTCCGGCA CAACTGCCGC
    AGCGAGTTCA ATGACGCCAT CGTCATCTCC AACCGGGCCC TGCGGGACGG AGAGCTGTTT
    GAAATCGTTA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACT
    GCCATTCGGC CGGAAGACCT CGAATTCCCC AACACCATGA CAGACATTGA CTACGACACA
    TGGATGCTGA GTGGCACGGC CATCATGCAA GACGGGAACA CGATGCGCAA CAACTACGGG
    TGTGACCTGG ACGCCCTGGG CACGGGTGCA CGCATCGGCA TGATGCGAAC AGCCAAGGGT
    GACCTGCACT ACTTCATCAA CGGCCAGGAC CAAGGCGCCG CCTGCTCAGG CTTGCCTCCG
    GGTAAAGAGG TGTACGCAGT AGTGGATCTT TACGGCCAGT GTGTCCAAGT GTCCATCACC
    AATGCCACCG GCCCCATGGA CAACAGCTTG GCAACCAGCA ACACTGCCAC CGAGAAATCC
    TTCCCTTTGC ACTCCCCAGC GGCTGGCGTG GCTCACCGAT TCCACAGTAC TTGCGGCAAG
    AATGTGGCCC TGGAGGAGGA TGGCACAAGG GCGGTACGTG CGGTGGGCTA TGCGCATGGC
    CTGGTCTTCA GCACCAAGGA ACTCAAGACG GAGGAAGTAT TTGAGGTGAA AGTAGAGGAA
    CTGGATGAGA AGTGGGCGGG CTCCCTCCGG CTGGGGCTGA CCACACTAGC GCCCGGGGAG
    ATGGGGCCTG GCGCAGGTGG GGGCCCAGGG CTGCCTCCCT CCCTCCCGGA GCTCCGGACA
    AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGTG ACGGGCAGCT CCAGAGGATG
    AACTACGGCC GGAGCCTGGA GAGGCTGGGG GTGGGGAGCC GCGTGGGCAT TCGTCGGGGC
    GCAGATGACA CGATGCACAT CCTGGTGGAT GGAGAGGACA TGGGGCCCGC AGCTACCGGC
    ATTGCCAAGA ATGTGTGGGC CGTGCTGGAT CTCTACGGGC CGGTACGGAG TGTGTCTGTG
    GTCAGCTCCA CAAGGCTGGA GGAGCCCGAG GGCACCCAGC CTCCATCTCC CAGCTCGGAC
    ACGGGCAGCG AGGCCGAGGA AGATGACGAT GGGGAGGAGC ACAGCTTGAG AGGTCAGAGT
    CAAGTGGCCA TGATGTCGAC GTCACTGGAG TTCCTGGAGA ACCATGGGAA GAATATCCTT
    TTGTCCAACG GGAACCGTAC GGCCACACGC GTAGCCAGCT ACAACCAGGG CATCGTTGTC
    ATCAACCAGC CCCTGGTGCC CCAGCTCCTA GTCCAGGTGC GGATAGACTT CCTGAACCGG
    CAGTGGACAT CTTCCCTGGT CCTGGGAGTC ATCACCTGCC CGCCTGAGAG GCTCAACTTC
    CCTGCTTCTG CCTGCGCCCT CAAACGGGCA GCCTGGCTGC TGCGGGGCCG GGGCATCTTC
    CACAATGGTC TCAAGATTTG TGAGAAGTTT GGGCCAAATC TGGACACGTG TCCTGAAGGC
    ACCATCCTGG GGCTGCGGCT AGACAGCTCT GGGGGCCTGC ATCTCCACGT CAATGGGATG
    GACCAGGGGG TGGCGGTATC AGATGTCCCC CAGCCCTGCC ATGCGCTTGT GGACCTCTAT
    GGGCAGTGTG AGCAGGTGAC AATCGTGAGT CCTGAACCAG GGGCAGCCAG TGGGAAGAGT
    GCTGGAACCC AAGGGGACAT GGAGAAAGCG GACATGGTGG ATGGTATCAA GGAAAGTGTG
    TGCTGGGGCC CCCTGCCAGC TGCCAGCCCT CTCAAGAGCT GCGAGTACCA CGCCCTCTGC
    TCCCGCTTCC AAGAACTGCT GTTGCTTCCT GAGGACTATT TCATGCCTCC ACCCAAGCGG
    AGCCTGTGCT ACTGTGAGTC TTGCCGGAAG CTTCGAGGAG GCTCCCAAGA GGGTGCGTGG
    AAGAAGCCAC AGAACCTTTA CTGGTCGAGG GGAGGATCTT TGTCCAGTCT GCCCGGTCTA
    CACCCTAGGG TGAATCCCCG CCTGGAAGCT GGAACGATGA CTAAGAAGTG GCACATGGCA
    TATCACGGGA GCAACGTGGC AGCCATCCGG AGGGTGCTAG ACCGAGGAGA GCTGGGAGCA
    GGCACCGCCT CCATCCTGAG CTGCCGGCCC TTGAAAGGCA AGCCTGGGGT GGGGTTTGAG
    GAGCCTGGCG AGAACTGCGC CCCACCCCGG GAGGAGCAGC CCCCTTCCGT CTTGCTGTCC
    CCCTCCCTCC AGTATGCTGG GGCCGAGACC CTAGCGTCCA AAGTGCAATT CCGGGATCCC
    AAGTCCCAGC GAACGCACCA GGCCCAGGTG GCGTTCCAGG TGTGCGTGCG CCCGGGCTCC
    TACACCCCCG GACCCCCTTC GGCCACCCTC CGAGAACCTC CCGACCCTCA CTTCAGCCCC
    GCAGAACTCG AGTGGATAAC CAAGGAGAAG GGGGCCACAC TCCTCTATGC CCTGCTGGTG
    CGGGTGGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288107
    Clone ID Related Accession (Same CDS sequence) XM_022418492.1
    Accession Version XM_022418492.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neuralized-like protein 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGTTTCTG GGGAGGTGAA AAGATGGAGG GATGTCGGGG ATGTGGTAGG AGCGCAGGAG 
    AGAAGCCGGG GAAGGACCGG CCGTTGGAGG ATCAGCTGGG GCGAAGAGGT CAACTCCTGG
    AGTGGCTCCA TTGAGATTGG GGTGACGGCC CTAGACCCCA GTGTTCTGGA CTTCCCAAGC
    AGCGCCACAG GGCTGAAGGG GGGCTCATGG GTAGTGTCAG GCTGCTCGGT GTTGAGGGAT
    GGACGCTCTG TGCTGGAGGA GTATGGTCAG GACCTGGACC AGCTTGGTGA AGGGGACCGT
    GTGGGCGTGG AGCGCACAGT TGCTGGGGAG CTGCGGCTCT GGGTGAATGG GCGGGACTGT
    GGTGTTGCCG CCACGGGCCT CCCCGCCCGG GTCTGGGCCG TGGTGGACCT TTACGGCAAG
    TGCACCCAGA TCACCGTGCT GCCCCCCGAG CCAGGTTTCA GCCCCCCTGC TCCCATCCCC
    ACACCTCCCC TCGAGCCCTC TGCACCCCCT GAAGATTCCA CCTTGACTGA ACAGGCGACC
    TCTGGGGATG AAGCCTTCAT GGTGTCCCCA GCGCAGCCCC GGCCGGAGAC GTTTCCTAAC
    AGCCTTGAGT CGCATAATGA CTTTGCCAGC ATGGAGCTCT CTGAGGTGGT GGGCAACGCC
    ATCCTTTCTG CCTACAACGG GGGGCTCCTA AATGTGAACC TGAGCTCCCC ACCGGCAGGG
    GAAGGACTGG GGTCTGGCGG GGCTGCCACC TCGCCCATCC TCACCTCCAA CGACGCCCTG
    CTCTTTCACG AGAAGTGTGG GACTCTCATC AAACTCAGCA ACAACAATAA GACAGCTGAG
    CGCCGCCGGC CCCTGGACGA ATTCAACAAC GGGGTTGTCA TGACCAACCG CCCTCTCCGG
    GACAATGAGA TGTTTGAGAT CCGCATTGAC AAGCTCGTAG ACAAGTGGTC GGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCCAAC AACCTGGAGT ACCCTGCCAC CATGACCAAT
    TTACAGTCAG GCACCATCAT GATGAGCGGC TGTGGGATCC TGACCAATGG CAAGGGCACC
    CGCCGGGAGT ACTGTGAATT CAGCCTGGAT GAGCTGCAGG AGGGCGACCA CATTGGTCTC
    ACAAGGAAGT CCAACTCAGC CCTGCACTTC TTTATCAATG GCATTGATCA GGGCGTGGCT
    ACCCCACTGA CACCCCCAGT GGTGTACGGC GTGGTGGACT TGTACGGGAT GGCTGTGAAG
    GTGACCATCG TCCACAATAA CAACCACAGC GACCGGCTGC GTCGAAACAA TGCCATCCTG
    CGGGCACTGT CCCCCGAGGG CGCCCTGCGT CGCGCCACTC CTGCCACCCA GGCAGAGCCC
    GAGCGCCTGC TCTTCCACCC TAACTGCGGG CAGAAGGCAG CTATCACCCA CGAGGGACGC
    ACTGCCCTGA GGCCCCAGTA TGGCCCATGG TGTGGGGAGA AGCCTGCTGC AGGGACTCTA
    ACAGGATCGG AGGAGGGGTG GAGAAGCAGG AGTGCCACAG ATGACTTCAA CCATGGCGTG
    GTGCTGAGTA GCAGAGCCCT GCGGGACGGA GAGGTGTTTC AGGTGCGCAT CGACAAGATG
    GTGGACAAGT GGGCTGGCTC CATTGAGATT GGTGTCACCA CCCACAACCC TGCCTACCTC
    CAGTTGCCCT CCACCATGAC CAACTTGCGC TCTGGGACCT GGATGATGAC CGGGAATGGG
    GTGATGCACA ACGGAACGAC CATTTTGGAC GAGTATGGGC ACAATCTGGA CCGCCTCAAG
    GTGGGGGAGC AGGCAGGGGA CACTGTGGGC GTGGTGCGGC GGGAGGACGG GACCCTGCAC
    TTCTTCGTCA ACGGGATGAC CCAGGGCCCT GCGGCCTGGA ATGTGCCCCC TGGCGTCTAC
    GCTGTTGTGG ACCTCTATGG CCAGGCAGCC CAAGCTACCA TTGTGGATGA CGTGGAGGTG
    CCCCCGGCCC CTGAGCCCCT CTCTGAAGGG AACAACCAGG TGTCTCCCAG CTCCCCATCA
    TCAGGGGCCG GGGGCTCTGA CCTGCGCTTC CATCAGCTGC ACGGCAGTAA TGCAGTCATC
    ACCAACGGGG GCCGCACCGC ACTCCGGCAC AACTGCCGCA GCGAGTTCAA TGACGCCATC
    GTCATCTCCA ACCGGGCCCT GCGGGACGGA GAGCTGTTTG AAATCGTTAT TCAGAAGATG
    GTGGACCGCT GGTCAGGCTC CATTGAGGCT GGAGTGACTG CCATTCGGCC GGAAGACCTC
    GAATTCCCCA ACACCATGAC AGACATTGAC TACGACACAT GGATGCTGAG TGGCACGGCC
    ATCATGCAAG ACGGGAACAC GATGCGCAAC AACTACGGGT GTGACCTGGA CGCCCTGGGC
    ACGGGTGCAC GCATCGGCAT GATGCGAACA GCCAAGGGTG ACCTGCACTA CTTCATCAAC
    GGCCAGGACC AAGGCGCCGC CTGCTCAGGC TTGCCTCCGG GTAAAGAGGT GTACGCAGTA
    GTGGATCTTT ACGGCCAGTG TGTCCAAGTG TCCATCACCA ATGCCACCGG CCCCATGGAC
    AACAGCTTGG CAACCAGCAA CACTGCCACC GAGAAATCCT TCCCTTTGCA CTCCCCAGCG
    GCTGGCGTGG CTCACCGATT CCACAGTACT TGCGGCAAGA ATGTGGCCCT GGAGGAGGAT
    GGCACAAGGG CGGTACGTGC GGTGGGCTAT GCGCATGGCC TGGTCTTCAG CACCAAGGAA
    CTCAAGACGG AGGAAGTATT TGAGGTGAAA GTAGAGGAAC TGGATGAGAA GTGGGCGGGC
    TCCCTCCGGC TGGGGCTGAC CACACTAGCG CCCGGGGAGA TGGGGCCTGG CGCAGGTGGG
    GGCCCAGGGC TGCCTCCCTC CCTCCCGGAG CTCCGGACAA AGACCACCTG GATGGTGTCC
    AGCTGTGAAG TGAGGCGTGA CGGGCAGCTC CAGAGGATGA ACTACGGCCG GAGCCTGGAG
    AGGCTGGGGG TGGGGAGCCG CGTGGGCATT CGTCGGGGCG CAGATGACAC GATGCACATC
    CTGGTGGATG GAGAGGACAT GGGGCCCGCA GCTACCGGCA TTGCCAAGAA TGTGTGGGCC
    GTGCTGGATC TCTACGGGCC GGTACGGAGT GTGTCTGTGG TCAGCTCCAC AAGGCTGGAG
    GAGCCCGAGG GCACCCAGCC TCCATCTCCC AGCTCGGACA CGGGCAGCGA GGCCGAGGAA
    GATGACGATG GGGAGGAGCA CAGCTTGAGA GGTCAGAGTC AAGTGGCCAT GATGTCGACG
    TCACTGGAGT TCCTGGAGAA CCATGGGAAG AATATCCTTT TGTCCAACGG GAACCGTACG
    GCCACACGCG TAGCCAGCTA CAACCAGGGC ATCGTTGTCA TCAACCAGCC CCTGGTGCCC
    CAGCTCCTAG TCCAGGTGCG GATAGACTTC CTGAACCGGC AGTGGACATC TTCCCTGGTC
    CTGGGAGTCA TCACCTGCCC GCCTGAGAGG CTCAACTTCC CTGCTTCTGC CTGCGCCCTC
    AAACGGGCAG CCTGGCTGCT GCGGGGCCGG GGCATCTTCC ACAATGGTCT CAAGATTTGT
    GAGAAGTTTG GGCCAAATCT GGACACGTGT CCTGAAGGCA CCATCCTGGG GCTGCGGCTA
    GACAGCTCTG GGGGCCTGCA TCTCCACGTC AATGGGATGG ACCAGGGGGT GGCGGTATCA
    GATGTCCCCC AGCCCTGCCA TGCGCTTGTG GACCTCTATG GGCAGTGTGA GCAGGTGACA
    ATCGTGAGTC CTGAACCAGG GGCAGCCAGT GGGAAGAGTG CTGGAACCCA AGGGGACATG
    GAGAAAGCGG ACATGGTGGA TGGTATCAAG GAAAGTGTGT GCTGGGGCCC CCTGCCAGCT
    GCCAGCCCTC TCAAGAGCTG CGAGTACCAC GCCCTCTGCT CCCGCTTCCA AGAACTGCTG
    TTGCTTCCTG AGGACTATTT CATGCCTCCA CCCAAGCGGA GCCTGTGCTA CTGTGAGTCT
    TGCCGGAAGC TTCGAGGAGG CTCCCAAGAG GGTGCGTGGA AGAAGCCACA GAACCTTTAC
    TGGTCGAGGG GAGGATCTTT GTCCAGTCTG CCCGGTCTAC ACCCTAGGGT GAATCCCCGC
    CTGGAAGCTG GAACGATGAC TAAGAAGTGG CACATGGCAT ATCACGGGAG CAACGTGGCA
    GCCATCCGGA GGGTGCTAGA CCGAGGAGAG CTGGGAGCAG GCACCGCCTC CATCCTGAGC
    TGCCGGCCCT TGAAAGGCAA GCCTGGGGTG GGGTTTGAGG AGCCTGGCGA GAACTGCGCC
    CCACCCCGGG AGGAGCAGCC CCCTTCCGTC TTGCTGTCCC CCTCCCTCCA GTATGCTGGG
    GCCGAGACCC TAGCGTCCAA AGTGCAATTC CGGGATCCCA AGTCCCAGCG AACGCACCAG
    GCCCAGGTGG CGTTCCAGGT GTGCGTGCGC CCGGGCTCCT ACACCCCCGG ACCCCCTTCG
    GCCACCCTCC GAGAACCTCC CGACCCTCAC TTCAGCCCCG CAGAACTCGA GTGGATAACC
    AAGGAGAAGG GGGCCACACT CCTCTATGCC CTGCTGGTGC GGGTGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274200.1
    CDS153..4763
    Translation

    Target ORF information:

    RefSeq Version XM_022418492.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGTTTCTG GGGAGGTGAA AAGATGGAGG GATGTCGGGG ATGTGGTAGG AGCGCAGGAG 
    AGAAGCCGGG GAAGGACCGG CCGTTGGAGG ATCAGCTGGG GCGAAGAGGT CAACTCCTGG
    AGTGGCTCCA TTGAGATTGG GGTGACGGCC CTAGACCCCA GTGTTCTGGA CTTCCCAAGC
    AGCGCCACAG GGCTGAAGGG GGGCTCATGG GTAGTGTCAG GCTGCTCGGT GTTGAGGGAT
    GGACGCTCTG TGCTGGAGGA GTATGGTCAG GACCTGGACC AGCTTGGTGA AGGGGACCGT
    GTGGGCGTGG AGCGCACAGT TGCTGGGGAG CTGCGGCTCT GGGTGAATGG GCGGGACTGT
    GGTGTTGCCG CCACGGGCCT CCCCGCCCGG GTCTGGGCCG TGGTGGACCT TTACGGCAAG
    TGCACCCAGA TCACCGTGCT GCCCCCCGAG CCAGGTTTCA GCCCCCCTGC TCCCATCCCC
    ACACCTCCCC TCGAGCCCTC TGCACCCCCT GAAGATTCCA CCTTGACTGA ACAGGCGACC
    TCTGGGGATG AAGCCTTCAT GGTGTCCCCA GCGCAGCCCC GGCCGGAGAC GTTTCCTAAC
    AGCCTTGAGT CGCATAATGA CTTTGCCAGC ATGGAGCTCT CTGAGGTGGT GGGCAACGCC
    ATCCTTTCTG CCTACAACGG GGGGCTCCTA AATGTGAACC TGAGCTCCCC ACCGGCAGGG
    GAAGGACTGG GGTCTGGCGG GGCTGCCACC TCGCCCATCC TCACCTCCAA CGACGCCCTG
    CTCTTTCACG AGAAGTGTGG GACTCTCATC AAACTCAGCA ACAACAATAA GACAGCTGAG
    CGCCGCCGGC CCCTGGACGA ATTCAACAAC GGGGTTGTCA TGACCAACCG CCCTCTCCGG
    GACAATGAGA TGTTTGAGAT CCGCATTGAC AAGCTCGTAG ACAAGTGGTC GGGCTCCATT
    GAGATTGGTG TCACCACCCA CAACCCCAAC AACCTGGAGT ACCCTGCCAC CATGACCAAT
    TTACAGTCAG GCACCATCAT GATGAGCGGC TGTGGGATCC TGACCAATGG CAAGGGCACC
    CGCCGGGAGT ACTGTGAATT CAGCCTGGAT GAGCTGCAGG AGGGCGACCA CATTGGTCTC
    ACAAGGAAGT CCAACTCAGC CCTGCACTTC TTTATCAATG GCATTGATCA GGGCGTGGCT
    ACCCCACTGA CACCCCCAGT GGTGTACGGC GTGGTGGACT TGTACGGGAT GGCTGTGAAG
    GTGACCATCG TCCACAATAA CAACCACAGC GACCGGCTGC GTCGAAACAA TGCCATCCTG
    CGGGCACTGT CCCCCGAGGG CGCCCTGCGT CGCGCCACTC CTGCCACCCA GGCAGAGCCC
    GAGCGCCTGC TCTTCCACCC TAACTGCGGG CAGAAGGCAG CTATCACCCA CGAGGGACGC
    ACTGCCCTGA GGCCCCAGTA TGGCCCATGG TGTGGGGAGA AGCCTGCTGC AGGGACTCTA
    ACAGGATCGG AGGAGGGGTG GAGAAGCAGG AGTGCCACAG ATGACTTCAA CCATGGCGTG
    GTGCTGAGTA GCAGAGCCCT GCGGGACGGA GAGGTGTTTC AGGTGCGCAT CGACAAGATG
    GTGGACAAGT GGGCTGGCTC CATTGAGATT GGTGTCACCA CCCACAACCC TGCCTACCTC
    CAGTTGCCCT CCACCATGAC CAACTTGCGC TCTGGGACCT GGATGATGAC CGGGAATGGG
    GTGATGCACA ACGGAACGAC CATTTTGGAC GAGTATGGGC ACAATCTGGA CCGCCTCAAG
    GTGGGGGAGC AGGCAGGGGA CACTGTGGGC GTGGTGCGGC GGGAGGACGG GACCCTGCAC
    TTCTTCGTCA ACGGGATGAC CCAGGGCCCT GCGGCCTGGA ATGTGCCCCC TGGCGTCTAC
    GCTGTTGTGG ACCTCTATGG CCAGGCAGCC CAAGCTACCA TTGTGGATGA CGTGGAGGTG
    CCCCCGGCCC CTGAGCCCCT CTCTGAAGGG AACAACCAGG TGTCTCCCAG CTCCCCATCA
    TCAGGGGCCG GGGGCTCTGA CCTGCGCTTC CATCAGCTGC ACGGCAGTAA TGCAGTCATC
    ACCAACGGGG GCCGCACCGC ACTCCGGCAC AACTGCCGCA GCGAGTTCAA TGACGCCATC
    GTCATCTCCA ACCGGGCCCT GCGGGACGGA GAGCTGTTTG AAATCGTTAT TCAGAAGATG
    GTGGACCGCT GGTCAGGCTC CATTGAGGCT GGAGTGACTG CCATTCGGCC GGAAGACCTC
    GAATTCCCCA ACACCATGAC AGACATTGAC TACGACACAT GGATGCTGAG TGGCACGGCC
    ATCATGCAAG ACGGGAACAC GATGCGCAAC AACTACGGGT GTGACCTGGA CGCCCTGGGC
    ACGGGTGCAC GCATCGGCAT GATGCGAACA GCCAAGGGTG ACCTGCACTA CTTCATCAAC
    GGCCAGGACC AAGGCGCCGC CTGCTCAGGC TTGCCTCCGG GTAAAGAGGT GTACGCAGTA
    GTGGATCTTT ACGGCCAGTG TGTCCAAGTG TCCATCACCA ATGCCACCGG CCCCATGGAC
    AACAGCTTGG CAACCAGCAA CACTGCCACC GAGAAATCCT TCCCTTTGCA CTCCCCAGCG
    GCTGGCGTGG CTCACCGATT CCACAGTACT TGCGGCAAGA ATGTGGCCCT GGAGGAGGAT
    GGCACAAGGG CGGTACGTGC GGTGGGCTAT GCGCATGGCC TGGTCTTCAG CACCAAGGAA
    CTCAAGACGG AGGAAGTATT TGAGGTGAAA GTAGAGGAAC TGGATGAGAA GTGGGCGGGC
    TCCCTCCGGC TGGGGCTGAC CACACTAGCG CCCGGGGAGA TGGGGCCTGG CGCAGGTGGG
    GGCCCAGGGC TGCCTCCCTC CCTCCCGGAG CTCCGGACAA AGACCACCTG GATGGTGTCC
    AGCTGTGAAG TGAGGCGTGA CGGGCAGCTC CAGAGGATGA ACTACGGCCG GAGCCTGGAG
    AGGCTGGGGG TGGGGAGCCG CGTGGGCATT CGTCGGGGCG CAGATGACAC GATGCACATC
    CTGGTGGATG GAGAGGACAT GGGGCCCGCA GCTACCGGCA TTGCCAAGAA TGTGTGGGCC
    GTGCTGGATC TCTACGGGCC GGTACGGAGT GTGTCTGTGG TCAGCTCCAC AAGGCTGGAG
    GAGCCCGAGG GCACCCAGCC TCCATCTCCC AGCTCGGACA CGGGCAGCGA GGCCGAGGAA
    GATGACGATG GGGAGGAGCA CAGCTTGAGA GGTCAGAGTC AAGTGGCCAT GATGTCGACG
    TCACTGGAGT TCCTGGAGAA CCATGGGAAG AATATCCTTT TGTCCAACGG GAACCGTACG
    GCCACACGCG TAGCCAGCTA CAACCAGGGC ATCGTTGTCA TCAACCAGCC CCTGGTGCCC
    CAGCTCCTAG TCCAGGTGCG GATAGACTTC CTGAACCGGC AGTGGACATC TTCCCTGGTC
    CTGGGAGTCA TCACCTGCCC GCCTGAGAGG CTCAACTTCC CTGCTTCTGC CTGCGCCCTC
    AAACGGGCAG CCTGGCTGCT GCGGGGCCGG GGCATCTTCC ACAATGGTCT CAAGATTTGT
    GAGAAGTTTG GGCCAAATCT GGACACGTGT CCTGAAGGCA CCATCCTGGG GCTGCGGCTA
    GACAGCTCTG GGGGCCTGCA TCTCCACGTC AATGGGATGG ACCAGGGGGT GGCGGTATCA
    GATGTCCCCC AGCCCTGCCA TGCGCTTGTG GACCTCTATG GGCAGTGTGA GCAGGTGACA
    ATCGTGAGTC CTGAACCAGG GGCAGCCAGT GGGAAGAGTG CTGGAACCCA AGGGGACATG
    GAGAAAGCGG ACATGGTGGA TGGTATCAAG GAAAGTGTGT GCTGGGGCCC CCTGCCAGCT
    GCCAGCCCTC TCAAGAGCTG CGAGTACCAC GCCCTCTGCT CCCGCTTCCA AGAACTGCTG
    TTGCTTCCTG AGGACTATTT CATGCCTCCA CCCAAGCGGA GCCTGTGCTA CTGTGAGTCT
    TGCCGGAAGC TTCGAGGAGG CTCCCAAGAG GGTGCGTGGA AGAAGCCACA GAACCTTTAC
    TGGTCGAGGG GAGGATCTTT GTCCAGTCTG CCCGGTCTAC ACCCTAGGGT GAATCCCCGC
    CTGGAAGCTG GAACGATGAC TAAGAAGTGG CACATGGCAT ATCACGGGAG CAACGTGGCA
    GCCATCCGGA GGGTGCTAGA CCGAGGAGAG CTGGGAGCAG GCACCGCCTC CATCCTGAGC
    TGCCGGCCCT TGAAAGGCAA GCCTGGGGTG GGGTTTGAGG AGCCTGGCGA GAACTGCGCC
    CCACCCCGGG AGGAGCAGCC CCCTTCCGTC TTGCTGTCCC CCTCCCTCCA GTATGCTGGG
    GCCGAGACCC TAGCGTCCAA AGTGCAATTC CGGGATCCCA AGTCCCAGCG AACGCACCAG
    GCCCAGGTGG CGTTCCAGGT GTGCGTGCGC CCGGGCTCCT ACACCCCCGG ACCCCCTTCG
    GCCACCCTCC GAGAACCTCC CGACCCTCAC TTCAGCCCCG CAGAACTCGA GTGGATAACC
    AAGGAGAAGG GGGCCACACT CCTCTATGCC CTGCTGGTGC GGGTGGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36406
    Clone ID Related Accession (Same CDS sequence) XM_014113521.1
    Accession Version XM_014113521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuralized-like protein 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGTGGGGG AGCAGGCAGG GGACACTGTG
    GGCGTGGTGC GGCGGGAGGA CGGGACCCTG CACTTCTTCG TCAACGGGAT GACCCAGGGC
    CCTGCGGCCT GGAATGTGCC CCCTGGCGTC TACGCTGTTG TGGACCTCTA TGGCCAGGCA
    GCCCAAGCTA CCATTGTGGA TGACGTGGAG GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA
    GGGAACAACC AGGTGTCTCC CAGCTCCCCA TCATCAGGGG CCGGGGGCTC TGACCTGCGC
    TTCCATCAGC TGCACGGCAG TAATGCAGTC ATCACCAACG GGGGCCGCAC CGCACTCCGG
    CACAACTGCC GCAGCGAGTT CAATGACGCC ATCGTCATCT CCAACCGGGC CCTGCGGGAC
    GGAGAGCTGT TTGAAATCGT TATTCAGAAG ATGGTGGACC GCTGGTCAGG CTCCATTGAG
    GCTGGAGTGA CTGCCATTCG GCCGGAAGAC CTCGAATTCC CCAACACCAT GACAGACATT
    GACTACGACA CATGGATGCT GAGTGGCACG GCCATCATGC AAGACGGGAA CACGATGCGC
    AACAACTACG GGTGTGACCT GGACGCCCTG GGCACGGGTG CACGCATCGG CATGATGCGA
    ACAGCCAAGG GTGACCTGCA CTACTTCATC AACGGCCAGG ACCAAGGCGC CGCCTGCTCA
    GGCTTGCCTC CGGGTAAAGA GGTGTACGCA GTAGTGGATC TTTACGGCCA GTGTGTCCAA
    GTGTCCATCA CCAATGCCAC CGGCCCCATG GACAACAGCT TGGCAACCAG CAACACTGCC
    ACCGAGAAAT CCTTCCCTTT GCACTCCCCA GCGGCTGGCG TGGCTCACCG ATTCCACAGT
    ACTTGCGGCA AGAATGTGGC CCTGGAGGAG GATGGCACAA GGGCGGTACG TGCGGTGGGC
    TATGCGCATG GCCTGGTCTT CAGCACCAAG GAACTCAAGA CGGAGGAAGT ATTTGAGGTG
    AAAGTAGAGG AACTGGATGA GAAGTGGGCG GGCTCCCTCC GGCTGGGGCT GACCACACTA
    GCGCCCGGGG AGATGGGGCC TGGCGCAGGT GGGGGCCCAG GGCTGCCTCC CTCCCTCCCG
    GAGCTCCGGA CAAAGACCAC CTGGATGGTG TCCAGCTGTG AAGTGAGGCG TGACGGGCAG
    CTCCAGAGGA TGAACTACGG CCGGAGCCTG GAGAGGCTGG GGGTGGGGAG CCGCGTGGGC
    ATTCGTCGGG GCGCAGATGA CACGATGCAC ATCCTGGTGG ATGGAGAGGA CATGGGGCCC
    GCAGCTACCG GCATTGCCAA GAATGTGTGG GCCGTGCTGG ATCTCTACGG GCCGGTACGG
    AGTGTGTCTG TGGTCAGCTC CACAAGGCTG GAGGAGCCCG AGGGCACCCA GCCTCCATCT
    CCCAGCTCGG ACACGGGCAG CGAGGCCGAG GAAGATGACG ATGGGGAGGA GCACAGCTTG
    AGAGGTCAGA GTCAAGTGGC CATGATGTCG ACGTCACTGG AGTTCCTGGA GAACCATGGG
    AAGAATATCC TTTTGTCCAA CGGGAACCGT ACGGCCACAC GCGTAGCCAG CTACAACCAG
    GGCATCGTTG TCATCAACCA GCCCCTGGTG CCCCAGCTCC TAGTCCAGGT GCGGATAGAC
    TTCCTGAACC GGCAGTGGAC ATCTTCCCTG GTCCTGGGAG TCATCACCTG CCCGCCTGAG
    AGGCTCAACT TCCCTGCTTC TGCCTGCGCC CTCAAACGGG CAGCCTGGCT GCTGCGGGGC
    CGGGGCATCT TCCACAATGG TCTCAAGATT TGTGAGAAGT TTGGGCCAAA TCTGGACACG
    TGTCCTGAAG GCACCATCCT GGGGCTGCGG CTAGACAGCT CTGGGGGCCT GCATCTCCAC
    GTCAATGGGA TGGACCAGGG GGTGGCGGTA TCAGATGTCC CCCAGCCCTG CCATGCGCTT
    GTGGACCTCT ATGGGCAGTG TGAGCAGGTG ACAATCGTGA GTCCTGAACC AGGGGCAGCC
    AGTGGGAAGA GTGCTGGAAC CCAAGGGGAC ATGGAGAAAG CGGACATGGT GGATGGTATC
    AAGGAAAGTG TGTGCTGGGG CCCCCTGCCA GCTGCCAGCC CTCTCAAGAG CTGCGAGTAC
    CACGCCCTCT GCTCCCGCTT CCAAGAACTG CTGTTGCTTC CTGAGGACTA TTTCATGCCT
    CCACCCAAGC GGAGCCTGTG CTACTGTGAG TCTTGCCGGA AGCTTCGAGG TGACGAGGCC
    CACAGGCGCC GTGGGGAGCC CCCCCGGGAA TACGCGCTGC CCTTCGGCTG GTGCAGGTTC
    AATCTCAGGG TGAATCCCCG CCTGGAAGCT GGAACGATGA CTAAGAAGTG GCACATGGCA
    TATCACGGGA GCAACGTGGC AGCCATCCGG AGGGTGCTAG ACCGAGGAGA GCTGGGAGCA
    GGCACCGCCT CCATCCTGAG CTGCCGGCCC TTGAAAGGCA AGCCTGGGGT GGGGTTTGAG
    GAGCCTGGCG AGAACTGCGC CCCACCCCGG GAGGAGCAGC CCCCTTCCGT CTTGCTGTCC
    CCCTCCCTCC AGTATGCTGG GGCCGAGACC CTAGCGTCCA AAGTGCAATT CCGGGATCCC
    AAGTCCCAGC GAACGCACCA GGCCCAGGTG GCGTTCCAGG TGTGCGTGCG CCCGGGCTCC
    TACACCCCCG GACCCCCTTC GGCCACCCTC CGAGAACCTC CCGACCCTCA CTTCAGCCCC
    GCAGAACTCG AGTGGATAAC CAAGGAGAAG GGGGCCACAC TCCTCTATGC CCTGCTGGTG
    CGGGTGGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968996.1
    CDS370..5061
    Translation

    Target ORF information:

    RefSeq Version XM_014113521.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGTGGGGG AGCAGGCAGG GGACACTGTG
    GGCGTGGTGC GGCGGGAGGA CGGGACCCTG CACTTCTTCG TCAACGGGAT GACCCAGGGC
    CCTGCGGCCT GGAATGTGCC CCCTGGCGTC TACGCTGTTG TGGACCTCTA TGGCCAGGCA
    GCCCAAGCTA CCATTGTGGA TGACGTGGAG GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA
    GGGAACAACC AGGTGTCTCC CAGCTCCCCA TCATCAGGGG CCGGGGGCTC TGACCTGCGC
    TTCCATCAGC TGCACGGCAG TAATGCAGTC ATCACCAACG GGGGCCGCAC CGCACTCCGG
    CACAACTGCC GCAGCGAGTT CAATGACGCC ATCGTCATCT CCAACCGGGC CCTGCGGGAC
    GGAGAGCTGT TTGAAATCGT TATTCAGAAG ATGGTGGACC GCTGGTCAGG CTCCATTGAG
    GCTGGAGTGA CTGCCATTCG GCCGGAAGAC CTCGAATTCC CCAACACCAT GACAGACATT
    GACTACGACA CATGGATGCT GAGTGGCACG GCCATCATGC AAGACGGGAA CACGATGCGC
    AACAACTACG GGTGTGACCT GGACGCCCTG GGCACGGGTG CACGCATCGG CATGATGCGA
    ACAGCCAAGG GTGACCTGCA CTACTTCATC AACGGCCAGG ACCAAGGCGC CGCCTGCTCA
    GGCTTGCCTC CGGGTAAAGA GGTGTACGCA GTAGTGGATC TTTACGGCCA GTGTGTCCAA
    GTGTCCATCA CCAATGCCAC CGGCCCCATG GACAACAGCT TGGCAACCAG CAACACTGCC
    ACCGAGAAAT CCTTCCCTTT GCACTCCCCA GCGGCTGGCG TGGCTCACCG ATTCCACAGT
    ACTTGCGGCA AGAATGTGGC CCTGGAGGAG GATGGCACAA GGGCGGTACG TGCGGTGGGC
    TATGCGCATG GCCTGGTCTT CAGCACCAAG GAACTCAAGA CGGAGGAAGT ATTTGAGGTG
    AAAGTAGAGG AACTGGATGA GAAGTGGGCG GGCTCCCTCC GGCTGGGGCT GACCACACTA
    GCGCCCGGGG AGATGGGGCC TGGCGCAGGT GGGGGCCCAG GGCTGCCTCC CTCCCTCCCG
    GAGCTCCGGA CAAAGACCAC CTGGATGGTG TCCAGCTGTG AAGTGAGGCG TGACGGGCAG
    CTCCAGAGGA TGAACTACGG CCGGAGCCTG GAGAGGCTGG GGGTGGGGAG CCGCGTGGGC
    ATTCGTCGGG GCGCAGATGA CACGATGCAC ATCCTGGTGG ATGGAGAGGA CATGGGGCCC
    GCAGCTACCG GCATTGCCAA GAATGTGTGG GCCGTGCTGG ATCTCTACGG GCCGGTACGG
    AGTGTGTCTG TGGTCAGCTC CACAAGGCTG GAGGAGCCCG AGGGCACCCA GCCTCCATCT
    CCCAGCTCGG ACACGGGCAG CGAGGCCGAG GAAGATGACG ATGGGGAGGA GCACAGCTTG
    AGAGGTCAGA GTCAAGTGGC CATGATGTCG ACGTCACTGG AGTTCCTGGA GAACCATGGG
    AAGAATATCC TTTTGTCCAA CGGGAACCGT ACGGCCACAC GCGTAGCCAG CTACAACCAG
    GGCATCGTTG TCATCAACCA GCCCCTGGTG CCCCAGCTCC TAGTCCAGGT GCGGATAGAC
    TTCCTGAACC GGCAGTGGAC ATCTTCCCTG GTCCTGGGAG TCATCACCTG CCCGCCTGAG
    AGGCTCAACT TCCCTGCTTC TGCCTGCGCC CTCAAACGGG CAGCCTGGCT GCTGCGGGGC
    CGGGGCATCT TCCACAATGG TCTCAAGATT TGTGAGAAGT TTGGGCCAAA TCTGGACACG
    TGTCCTGAAG GCACCATCCT GGGGCTGCGG CTAGACAGCT CTGGGGGCCT GCATCTCCAC
    GTCAATGGGA TGGACCAGGG GGTGGCGGTA TCAGATGTCC CCCAGCCCTG CCATGCGCTT
    GTGGACCTCT ATGGGCAGTG TGAGCAGGTG ACAATCGTGA GTCCTGAACC AGGGGCAGCC
    AGTGGGAAGA GTGCTGGAAC CCAAGGGGAC ATGGAGAAAG CGGACATGGT GGATGGTATC
    AAGGAAAGTG TGTGCTGGGG CCCCCTGCCA GCTGCCAGCC CTCTCAAGAG CTGCGAGTAC
    CACGCCCTCT GCTCCCGCTT CCAAGAACTG CTGTTGCTTC CTGAGGACTA TTTCATGCCT
    CCACCCAAGC GGAGCCTGTG CTACTGTGAG TCTTGCCGGA AGCTTCGAGG TGACGAGGCC
    CACAGGCGCC GTGGGGAGCC CCCCCGGGAA TACGCGCTGC CCTTCGGCTG GTGCAGGTTC
    AATCTCAGGG TGAATCCCCG CCTGGAAGCT GGAACGATGA CTAAGAAGTG GCACATGGCA
    TATCACGGGA GCAACGTGGC AGCCATCCGG AGGGTGCTAG ACCGAGGAGA GCTGGGAGCA
    GGCACCGCCT CCATCCTGAG CTGCCGGCCC TTGAAAGGCA AGCCTGGGGT GGGGTTTGAG
    GAGCCTGGCG AGAACTGCGC CCCACCCCGG GAGGAGCAGC CCCCTTCCGT CTTGCTGTCC
    CCCTCCCTCC AGTATGCTGG GGCCGAGACC CTAGCGTCCA AAGTGCAATT CCGGGATCCC
    AAGTCCCAGC GAACGCACCA GGCCCAGGTG GCGTTCCAGG TGTGCGTGCG CCCGGGCTCC
    TACACCCCCG GACCCCCTTC GGCCACCCTC CGAGAACCTC CCGACCCTCA CTTCAGCCCC
    GCAGAACTCG AGTGGATAAC CAAGGAGAAG GGGGCCACAC TCCTCTATGC CCTGCTGGTG
    CGGGTGGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36407
    Clone ID Related Accession (Same CDS sequence) XM_014113522.1
    Accession Version XM_014113522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuralized-like protein 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGTGGGGG AGCAGGCAGG GGACACTGTG
    GGCGTGGTGC GGCGGGAGGA CGGGACCCTG CACTTCTTCG TCAACGGGAT GACCCAGGGC
    CCTGCGGCCT GGAATGTGCC CCCTGGCGTC TACGCTGTTG TGGACCTCTA TGGCCAGGCA
    GCCCAAGCTA CCATTGTGGA TGACGTGGAG GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA
    GGGAACAACC AGGTGTCTCC CAGCTCCCCA TCATCAGGGG CCGGGGGCTC TGACCTGCGC
    TTCCATCAGC TGCACGGCAG TAATGCAGTC ATCACCAACG GGGGCCGCAC CGCACTCCGG
    CACAACTGCC GCAGCGAGTT CAATGACGCC ATCGTCATCT CCAACCGGGC CCTGCGGGAC
    GGAGAGCTGT TTGAAATCGT TATTCAGAAG ATGGTGGACC GCTGGTCAGG CTCCATTGAG
    GCTGGAGTGA CTGCCATTCG GCCGGAAGAC CTCGAATTCC CCAACACCAT GACAGACATT
    GACTACGACA CATGGATGCT GAGTGGCACG GCCATCATGC AAGACGGGAA CACGATGCGC
    AACAACTACG GGTGTGACCT GGACGCCCTG GGCACGGGTG CACGCATCGG CATGATGCGA
    ACAGCCAAGG GTGACCTGCA CTACTTCATC AACGGCCAGG ACCAAGGCGC CGCCTGCTCA
    GGCTTGCCTC CGGAGGTGTA CGCAGTAGTG GATCTTTACG GCCAGTGTGT CCAAGTGTCC
    ATCACCAATG CCACCGGCCC CATGGACAAC AGCTTGGCAA CCAGCAACAC TGCCACCGAG
    AAATCCTTCC CTTTGCACTC CCCAGCGGCT GGCGTGGCTC ACCGATTCCA CAGTACTTGC
    GGCAAGAATG TGGCCCTGGA GGAGGATGGC ACAAGGGCGG TACGTGCGGT GGGCTATGCG
    CATGGCCTGG TCTTCAGCAC CAAGGAACTC AAGACGGAGG AAGTATTTGA GGTGAAAGTA
    GAGGAACTGG ATGAGAAGTG GGCGGGCTCC CTCCGGCTGG GGCTGACCAC ACTAGCGCCC
    GGGGAGATGG GGCCTGGCGC AGGTGGGGGC CCAGGGCTGC CTCCCTCCCT CCCGGAGCTC
    CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGTGACGG GCAGCTCCAG
    AGGATGAACT ACGGCCGGAG CCTGGAGAGG CTGGGGGTGG GGAGCCGCGT GGGCATTCGT
    CGGGGCGCAG ATGACACGAT GCACATCCTG GTGGATGGAG AGGACATGGG GCCCGCAGCT
    ACCGGCATTG CCAAGAATGT GTGGGCCGTG CTGGATCTCT ACGGGCCGGT ACGGAGTGTG
    TCTGTGGTCA GCTCCACAAG GCTGGAGGAG CCCGAGGGCA CCCAGCCTCC ATCTCCCAGC
    TCGGACACGG GCAGCGAGGC CGAGGAAGAT GACGATGGGG AGGAGCACAG CTTGAGAGGT
    CAGAGTCAAG TGGCCATGAT GTCGACGTCA CTGGAGTTCC TGGAGAACCA TGGGAAGAAT
    ATCCTTTTGT CCAACGGGAA CCGTACGGCC ACACGCGTAG CCAGCTACAA CCAGGGCATC
    GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTCCTAGTCC AGGTGCGGAT AGACTTCCTG
    AACCGGCAGT GGACATCTTC CCTGGTCCTG GGAGTCATCA CCTGCCCGCC TGAGAGGCTC
    AACTTCCCTG CTTCTGCCTG CGCCCTCAAA CGGGCAGCCT GGCTGCTGCG GGGCCGGGGC
    ATCTTCCACA ATGGTCTCAA GATTTGTGAG AAGTTTGGGC CAAATCTGGA CACGTGTCCT
    GAAGGCACCA TCCTGGGGCT GCGGCTAGAC AGCTCTGGGG GCCTGCATCT CCACGTCAAT
    GGGATGGACC AGGGGGTGGC GGTATCAGAT GTCCCCCAGC CCTGCCATGC GCTTGTGGAC
    CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGTCCTG AACCAGGGGC AGCCAGTGGG
    AAGAGTGCTG GAACCCAAGG GGACATGGAG AAAGCGGACA TGGTGGATGG TATCAAGGAA
    AGTGTGTGCT GGGGCCCCCT GCCAGCTGCC AGCCCTCTCA AGAGCTGCGA GTACCACGCC
    CTCTGCTCCC GCTTCCAAGA ACTGCTGTTG CTTCCTGAGG ACTATTTCAT GCCTCCACCC
    AAGCGGAGCC TGTGCTACTG TGAGTCTTGC CGGAAGCTTC GAGGTGACGA GGCCCACAGG
    CGCCGTGGGG AGCCCCCCCG GGAATACGCG CTGCCCTTCG GCTGGTGCAG GTTCAATCTC
    AGGGTGAATC CCCGCCTGGA AGCTGGAACG ATGACTAAGA AGTGGCACAT GGCATATCAC
    GGGAGCAACG TGGCAGCCAT CCGGAGGGTG CTAGACCGAG GAGAGCTGGG AGCAGGCACC
    GCCTCCATCC TGAGCTGCCG GCCCTTGAAA GGCAAGCCTG GGGTGGGGTT TGAGGAGCCT
    GGCGAGAACT GCGCCCCACC CCGGGAGGAG CAGCCCCCTT CCGTCTTGCT GTCCCCCTCC
    CTCCAGTATG CTGGGGCCGA GACCCTAGCG TCCAAAGTGC AATTCCGGGA TCCCAAGTCC
    CAGCGAACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGCG TGCGCCCGGG CTCCTACACC
    CCCGGACCCC CTTCGGCCAC CCTCCGAGAA CCTCCCGACC CTCACTTCAG CCCCGCAGAA
    CTCGAGTGGA TAACCAAGGA GAAGGGGGCC ACACTCCTCT ATGCCCTGCT GGTGCGGGTG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968997.1
    CDS370..5055
    Translation

    Target ORF information:

    RefSeq Version XM_014113522.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGTGGGGG AGCAGGCAGG GGACACTGTG
    GGCGTGGTGC GGCGGGAGGA CGGGACCCTG CACTTCTTCG TCAACGGGAT GACCCAGGGC
    CCTGCGGCCT GGAATGTGCC CCCTGGCGTC TACGCTGTTG TGGACCTCTA TGGCCAGGCA
    GCCCAAGCTA CCATTGTGGA TGACGTGGAG GTGCCCCCGG CCCCTGAGCC CCTCTCTGAA
    GGGAACAACC AGGTGTCTCC CAGCTCCCCA TCATCAGGGG CCGGGGGCTC TGACCTGCGC
    TTCCATCAGC TGCACGGCAG TAATGCAGTC ATCACCAACG GGGGCCGCAC CGCACTCCGG
    CACAACTGCC GCAGCGAGTT CAATGACGCC ATCGTCATCT CCAACCGGGC CCTGCGGGAC
    GGAGAGCTGT TTGAAATCGT TATTCAGAAG ATGGTGGACC GCTGGTCAGG CTCCATTGAG
    GCTGGAGTGA CTGCCATTCG GCCGGAAGAC CTCGAATTCC CCAACACCAT GACAGACATT
    GACTACGACA CATGGATGCT GAGTGGCACG GCCATCATGC AAGACGGGAA CACGATGCGC
    AACAACTACG GGTGTGACCT GGACGCCCTG GGCACGGGTG CACGCATCGG CATGATGCGA
    ACAGCCAAGG GTGACCTGCA CTACTTCATC AACGGCCAGG ACCAAGGCGC CGCCTGCTCA
    GGCTTGCCTC CGGAGGTGTA CGCAGTAGTG GATCTTTACG GCCAGTGTGT CCAAGTGTCC
    ATCACCAATG CCACCGGCCC CATGGACAAC AGCTTGGCAA CCAGCAACAC TGCCACCGAG
    AAATCCTTCC CTTTGCACTC CCCAGCGGCT GGCGTGGCTC ACCGATTCCA CAGTACTTGC
    GGCAAGAATG TGGCCCTGGA GGAGGATGGC ACAAGGGCGG TACGTGCGGT GGGCTATGCG
    CATGGCCTGG TCTTCAGCAC CAAGGAACTC AAGACGGAGG AAGTATTTGA GGTGAAAGTA
    GAGGAACTGG ATGAGAAGTG GGCGGGCTCC CTCCGGCTGG GGCTGACCAC ACTAGCGCCC
    GGGGAGATGG GGCCTGGCGC AGGTGGGGGC CCAGGGCTGC CTCCCTCCCT CCCGGAGCTC
    CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGTGACGG GCAGCTCCAG
    AGGATGAACT ACGGCCGGAG CCTGGAGAGG CTGGGGGTGG GGAGCCGCGT GGGCATTCGT
    CGGGGCGCAG ATGACACGAT GCACATCCTG GTGGATGGAG AGGACATGGG GCCCGCAGCT
    ACCGGCATTG CCAAGAATGT GTGGGCCGTG CTGGATCTCT ACGGGCCGGT ACGGAGTGTG
    TCTGTGGTCA GCTCCACAAG GCTGGAGGAG CCCGAGGGCA CCCAGCCTCC ATCTCCCAGC
    TCGGACACGG GCAGCGAGGC CGAGGAAGAT GACGATGGGG AGGAGCACAG CTTGAGAGGT
    CAGAGTCAAG TGGCCATGAT GTCGACGTCA CTGGAGTTCC TGGAGAACCA TGGGAAGAAT
    ATCCTTTTGT CCAACGGGAA CCGTACGGCC ACACGCGTAG CCAGCTACAA CCAGGGCATC
    GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTCCTAGTCC AGGTGCGGAT AGACTTCCTG
    AACCGGCAGT GGACATCTTC CCTGGTCCTG GGAGTCATCA CCTGCCCGCC TGAGAGGCTC
    AACTTCCCTG CTTCTGCCTG CGCCCTCAAA CGGGCAGCCT GGCTGCTGCG GGGCCGGGGC
    ATCTTCCACA ATGGTCTCAA GATTTGTGAG AAGTTTGGGC CAAATCTGGA CACGTGTCCT
    GAAGGCACCA TCCTGGGGCT GCGGCTAGAC AGCTCTGGGG GCCTGCATCT CCACGTCAAT
    GGGATGGACC AGGGGGTGGC GGTATCAGAT GTCCCCCAGC CCTGCCATGC GCTTGTGGAC
    CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGTCCTG AACCAGGGGC AGCCAGTGGG
    AAGAGTGCTG GAACCCAAGG GGACATGGAG AAAGCGGACA TGGTGGATGG TATCAAGGAA
    AGTGTGTGCT GGGGCCCCCT GCCAGCTGCC AGCCCTCTCA AGAGCTGCGA GTACCACGCC
    CTCTGCTCCC GCTTCCAAGA ACTGCTGTTG CTTCCTGAGG ACTATTTCAT GCCTCCACCC
    AAGCGGAGCC TGTGCTACTG TGAGTCTTGC CGGAAGCTTC GAGGTGACGA GGCCCACAGG
    CGCCGTGGGG AGCCCCCCCG GGAATACGCG CTGCCCTTCG GCTGGTGCAG GTTCAATCTC
    AGGGTGAATC CCCGCCTGGA AGCTGGAACG ATGACTAAGA AGTGGCACAT GGCATATCAC
    GGGAGCAACG TGGCAGCCAT CCGGAGGGTG CTAGACCGAG GAGAGCTGGG AGCAGGCACC
    GCCTCCATCC TGAGCTGCCG GCCCTTGAAA GGCAAGCCTG GGGTGGGGTT TGAGGAGCCT
    GGCGAGAACT GCGCCCCACC CCGGGAGGAG CAGCCCCCTT CCGTCTTGCT GTCCCCCTCC
    CTCCAGTATG CTGGGGCCGA GACCCTAGCG TCCAAAGTGC AATTCCGGGA TCCCAAGTCC
    CAGCGAACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGCG TGCGCCCGGG CTCCTACACC
    CCCGGACCCC CTTCGGCCAC CCTCCGAGAA CCTCCCGACC CTCACTTCAG CCCCGCAGAA
    CTCGAGTGGA TAACCAAGGA GAAGGGGGCC ACACTCCTCT ATGCCCTGCT GGTGCGGGTG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36408
    Clone ID Related Accession (Same CDS sequence) XM_014113523.1
    Accession Version XM_014113523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuralized-like protein 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGCAGGGG ACACTGTGGG CGTGGTGCGG
    CGGGAGGACG GGACCCTGCA CTTCTTCGTC AACGGGATGA CCCAGGGCCC TGCGGCCTGG
    AATGTGCCCC CTGGCGTCTA CGCTGTTGTG GACCTCTATG GCCAGGCAGC CCAAGCTACC
    ATTGTGGATG ACGTGGAGGT GCCCCCGGCC CCTGAGCCCC TCTCTGAAGG GAACAACCAG
    GTGTCTCCCA GCTCCCCATC ATCAGGGGCC GGGGGCTCTG ACCTGCGCTT CCATCAGCTG
    CACGGCAGTA ATGCAGTCAT CACCAACGGG GGCCGCACCG CACTCCGGCA CAACTGCCGC
    AGCGAGTTCA ATGACGCCAT CGTCATCTCC AACCGGGCCC TGCGGGACGG AGAGCTGTTT
    GAAATCGTTA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACT
    GCCATTCGGC CGGAAGACCT CGAATTCCCC AACACCATGA CAGACATTGA CTACGACACA
    TGGATGCTGA GTGGCACGGC CATCATGCAA GACGGGAACA CGATGCGCAA CAACTACGGG
    TGTGACCTGG ACGCCCTGGG CACGGGTGCA CGCATCGGCA TGATGCGAAC AGCCAAGGGT
    GACCTGCACT ACTTCATCAA CGGCCAGGAC CAAGGCGCCG CCTGCTCAGG CTTGCCTCCG
    GGTAAAGAGG TGTACGCAGT AGTGGATCTT TACGGCCAGT GTGTCCAAGT GTCCATCACC
    AATGCCACCG GCCCCATGGA CAACAGCTTG GCAACCAGCA ACACTGCCAC CGAGAAATCC
    TTCCCTTTGC ACTCCCCAGC GGCTGGCGTG GCTCACCGAT TCCACAGTAC TTGCGGCAAG
    AATGTGGCCC TGGAGGAGGA TGGCACAAGG GCGGTACGTG CGGTGGGCTA TGCGCATGGC
    CTGGTCTTCA GCACCAAGGA ACTCAAGACG GAGGAAGTAT TTGAGGTGAA AGTAGAGGAA
    CTGGATGAGA AGTGGGCGGG CTCCCTCCGG CTGGGGCTGA CCACACTAGC GCCCGGGGAG
    ATGGGGCCTG GCGCAGGTGG GGGCCCAGGG CTGCCTCCCT CCCTCCCGGA GCTCCGGACA
    AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGTG ACGGGCAGCT CCAGAGGATG
    AACTACGGCC GGAGCCTGGA GAGGCTGGGG GTGGGGAGCC GCGTGGGCAT TCGTCGGGGC
    GCAGATGACA CGATGCACAT CCTGGTGGAT GGAGAGGACA TGGGGCCCGC AGCTACCGGC
    ATTGCCAAGA ATGTGTGGGC CGTGCTGGAT CTCTACGGGC CGGTACGGAG TGTGTCTGTG
    GTCAGCTCCA CAAGGCTGGA GGAGCCCGAG GGCACCCAGC CTCCATCTCC CAGCTCGGAC
    ACGGGCAGCG AGGCCGAGGA AGATGACGAT GGGGAGGAGC ACAGCTTGAG AGGTCAGAGT
    CAAGTGGCCA TGATGTCGAC GTCACTGGAG TTCCTGGAGA ACCATGGGAA GAATATCCTT
    TTGTCCAACG GGAACCGTAC GGCCACACGC GTAGCCAGCT ACAACCAGGG CATCGTTGTC
    ATCAACCAGC CCCTGGTGCC CCAGCTCCTA GTCCAGGTGC GGATAGACTT CCTGAACCGG
    CAGTGGACAT CTTCCCTGGT CCTGGGAGTC ATCACCTGCC CGCCTGAGAG GCTCAACTTC
    CCTGCTTCTG CCTGCGCCCT CAAACGGGCA GCCTGGCTGC TGCGGGGCCG GGGCATCTTC
    CACAATGGTC TCAAGATTTG TGAGAAGTTT GGGCCAAATC TGGACACGTG TCCTGAAGGC
    ACCATCCTGG GGCTGCGGCT AGACAGCTCT GGGGGCCTGC ATCTCCACGT CAATGGGATG
    GACCAGGGGG TGGCGGTATC AGATGTCCCC CAGCCCTGCC ATGCGCTTGT GGACCTCTAT
    GGGCAGTGTG AGCAGGTGAC AATCGTGAGT CCTGAACCAG GGGCAGCCAG TGGGAAGAGT
    GCTGGAACCC AAGGGGACAT GGAGAAAGCG GACATGGTGG ATGGTATCAA GGAAAGTGTG
    TGCTGGGGCC CCCTGCCAGC TGCCAGCCCT CTCAAGAGCT GCGAGTACCA CGCCCTCTGC
    TCCCGCTTCC AAGAACTGCT GTTGCTTCCT GAGGACTATT TCATGCCTCC ACCCAAGCGG
    AGCCTGTGCT ACTGTGAGTC TTGCCGGAAG CTTCGAGGTG ACGAGGCCCA CAGGCGCCGT
    GGGGAGCCCC CCCGGGAATA CGCGCTGCCC TTCGGCTGGT GCAGGTTCAA TCTCAGGGTG
    AATCCCCGCC TGGAAGCTGG AACGATGACT AAGAAGTGGC ACATGGCATA TCACGGGAGC
    AACGTGGCAG CCATCCGGAG GGTGCTAGAC CGAGGAGAGC TGGGAGCAGG CACCGCCTCC
    ATCCTGAGCT GCCGGCCCTT GAAAGGCAAG CCTGGGGTGG GGTTTGAGGA GCCTGGCGAG
    AACTGCGCCC CACCCCGGGA GGAGCAGCCC CCTTCCGTCT TGCTGTCCCC CTCCCTCCAG
    TATGCTGGGG CCGAGACCCT AGCGTCCAAA GTGCAATTCC GGGATCCCAA GTCCCAGCGA
    ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGCGTGCGCC CGGGCTCCTA CACCCCCGGA
    CCCCCTTCGG CCACCCTCCG AGAACCTCCC GACCCTCACT TCAGCCCCGC AGAACTCGAG
    TGGATAACCA AGGAGAAGGG GGCCACACTC CTCTATGCCC TGCTGGTGCG GGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968998.1
    CDS370..5049
    Translation

    Target ORF information:

    RefSeq Version XM_014113523.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCAGTATGGC
    CCATGTGCCA CAGATGACTT CAACCATGGC GTGGTGCTGA GTAGCAGAGC CCTGCGGGAC
    GGAGAGGTGT TTCAGGTGCG CATCGACAAG ATGGTGGACA AGTGGGCTGG CTCCATTGAG
    ATTGGTGTCA CCACCCACAA CCCTGCCTAC CTCCAGTTGC CCTCCACCAT GACCAACTTG
    CGCTCTGGGA CCTGGATGAT GACCGGGAAT GGGGTGATGC ACAACGGAAC GACCATTTTG
    GACGAGTATG GGCACAATCT GGACCGCCTC AAGGCAGGGG ACACTGTGGG CGTGGTGCGG
    CGGGAGGACG GGACCCTGCA CTTCTTCGTC AACGGGATGA CCCAGGGCCC TGCGGCCTGG
    AATGTGCCCC CTGGCGTCTA CGCTGTTGTG GACCTCTATG GCCAGGCAGC CCAAGCTACC
    ATTGTGGATG ACGTGGAGGT GCCCCCGGCC CCTGAGCCCC TCTCTGAAGG GAACAACCAG
    GTGTCTCCCA GCTCCCCATC ATCAGGGGCC GGGGGCTCTG ACCTGCGCTT CCATCAGCTG
    CACGGCAGTA ATGCAGTCAT CACCAACGGG GGCCGCACCG CACTCCGGCA CAACTGCCGC
    AGCGAGTTCA ATGACGCCAT CGTCATCTCC AACCGGGCCC TGCGGGACGG AGAGCTGTTT
    GAAATCGTTA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACT
    GCCATTCGGC CGGAAGACCT CGAATTCCCC AACACCATGA CAGACATTGA CTACGACACA
    TGGATGCTGA GTGGCACGGC CATCATGCAA GACGGGAACA CGATGCGCAA CAACTACGGG
    TGTGACCTGG ACGCCCTGGG CACGGGTGCA CGCATCGGCA TGATGCGAAC AGCCAAGGGT
    GACCTGCACT ACTTCATCAA CGGCCAGGAC CAAGGCGCCG CCTGCTCAGG CTTGCCTCCG
    GGTAAAGAGG TGTACGCAGT AGTGGATCTT TACGGCCAGT GTGTCCAAGT GTCCATCACC
    AATGCCACCG GCCCCATGGA CAACAGCTTG GCAACCAGCA ACACTGCCAC CGAGAAATCC
    TTCCCTTTGC ACTCCCCAGC GGCTGGCGTG GCTCACCGAT TCCACAGTAC TTGCGGCAAG
    AATGTGGCCC TGGAGGAGGA TGGCACAAGG GCGGTACGTG CGGTGGGCTA TGCGCATGGC
    CTGGTCTTCA GCACCAAGGA ACTCAAGACG GAGGAAGTAT TTGAGGTGAA AGTAGAGGAA
    CTGGATGAGA AGTGGGCGGG CTCCCTCCGG CTGGGGCTGA CCACACTAGC GCCCGGGGAG
    ATGGGGCCTG GCGCAGGTGG GGGCCCAGGG CTGCCTCCCT CCCTCCCGGA GCTCCGGACA
    AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGTG ACGGGCAGCT CCAGAGGATG
    AACTACGGCC GGAGCCTGGA GAGGCTGGGG GTGGGGAGCC GCGTGGGCAT TCGTCGGGGC
    GCAGATGACA CGATGCACAT CCTGGTGGAT GGAGAGGACA TGGGGCCCGC AGCTACCGGC
    ATTGCCAAGA ATGTGTGGGC CGTGCTGGAT CTCTACGGGC CGGTACGGAG TGTGTCTGTG
    GTCAGCTCCA CAAGGCTGGA GGAGCCCGAG GGCACCCAGC CTCCATCTCC CAGCTCGGAC
    ACGGGCAGCG AGGCCGAGGA AGATGACGAT GGGGAGGAGC ACAGCTTGAG AGGTCAGAGT
    CAAGTGGCCA TGATGTCGAC GTCACTGGAG TTCCTGGAGA ACCATGGGAA GAATATCCTT
    TTGTCCAACG GGAACCGTAC GGCCACACGC GTAGCCAGCT ACAACCAGGG CATCGTTGTC
    ATCAACCAGC CCCTGGTGCC CCAGCTCCTA GTCCAGGTGC GGATAGACTT CCTGAACCGG
    CAGTGGACAT CTTCCCTGGT CCTGGGAGTC ATCACCTGCC CGCCTGAGAG GCTCAACTTC
    CCTGCTTCTG CCTGCGCCCT CAAACGGGCA GCCTGGCTGC TGCGGGGCCG GGGCATCTTC
    CACAATGGTC TCAAGATTTG TGAGAAGTTT GGGCCAAATC TGGACACGTG TCCTGAAGGC
    ACCATCCTGG GGCTGCGGCT AGACAGCTCT GGGGGCCTGC ATCTCCACGT CAATGGGATG
    GACCAGGGGG TGGCGGTATC AGATGTCCCC CAGCCCTGCC ATGCGCTTGT GGACCTCTAT
    GGGCAGTGTG AGCAGGTGAC AATCGTGAGT CCTGAACCAG GGGCAGCCAG TGGGAAGAGT
    GCTGGAACCC AAGGGGACAT GGAGAAAGCG GACATGGTGG ATGGTATCAA GGAAAGTGTG
    TGCTGGGGCC CCCTGCCAGC TGCCAGCCCT CTCAAGAGCT GCGAGTACCA CGCCCTCTGC
    TCCCGCTTCC AAGAACTGCT GTTGCTTCCT GAGGACTATT TCATGCCTCC ACCCAAGCGG
    AGCCTGTGCT ACTGTGAGTC TTGCCGGAAG CTTCGAGGTG ACGAGGCCCA CAGGCGCCGT
    GGGGAGCCCC CCCGGGAATA CGCGCTGCCC TTCGGCTGGT GCAGGTTCAA TCTCAGGGTG
    AATCCCCGCC TGGAAGCTGG AACGATGACT AAGAAGTGGC ACATGGCATA TCACGGGAGC
    AACGTGGCAG CCATCCGGAG GGTGCTAGAC CGAGGAGAGC TGGGAGCAGG CACCGCCTCC
    ATCCTGAGCT GCCGGCCCTT GAAAGGCAAG CCTGGGGTGG GGTTTGAGGA GCCTGGCGAG
    AACTGCGCCC CACCCCGGGA GGAGCAGCCC CCTTCCGTCT TGCTGTCCCC CTCCCTCCAG
    TATGCTGGGG CCGAGACCCT AGCGTCCAAA GTGCAATTCC GGGATCCCAA GTCCCAGCGA
    ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGCGTGCGCC CGGGCTCCTA CACCCCCGGA
    CCCCCTTCGG CCACCCTCCG AGAACCTCCC GACCCTCACT TCAGCCCCGC AGAACTCGAG
    TGGATAACCA AGGAGAAGGG GGCCACACTC CTCTATGCCC TGCTGGTGCG GGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36409
    Clone ID Related Accession (Same CDS sequence) XM_014113524.1
    Accession Version XM_014113524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuralized-like protein 4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGTGGGGGAG CAGGCAGGGG ACACTGTGGG CGTGGTGCGG
    CGGGAGGACG GGACCCTGCA CTTCTTCGTC AACGGGATGA CCCAGGGCCC TGCGGCCTGG
    AATGTGCCCC CTGGCGTCTA CGCTGTTGTG GACCTCTATG GCCAGGCAGC CCAAGCTACC
    ATTGTGGATG ACGTGGAGGT GCCCCCGGCC CCTGAGCCCC TCTCTGAAGG GAACAACCAG
    GTGTCTCCCA GCTCCCCATC ATCAGGGGCC GGGGGCTCTG ACCTGCGCTT CCATCAGCTG
    CACGGCAGTA ATGCAGTCAT CACCAACGGG GGCCGCACCG CACTCCGGCA CAACTGCCGC
    AGCGAGTTCA ATGACGCCAT CGTCATCTCC AACCGGGCCC TGCGGGACGG AGAGCTGTTT
    GAAATCGTTA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACT
    GCCATTCGGC CGGAAGACCT CGAATTCCCC AACACCATGA CAGACATTGA CTACGACACA
    TGGATGCTGA GTGGCACGGC CATCATGCAA GACGGGAACA CGATGCGCAA CAACTACGGG
    TGTGACCTGG ACGCCCTGGG CACGGGTGCA CGCATCGGCA TGATGCGAAC AGCCAAGGGT
    GACCTGCACT ACTTCATCAA CGGCCAGGAC CAAGGCGCCG CCTGCTCAGG CTTGCCTCCG
    GGTAAAGAGG TGTACGCAGT AGTGGATCTT TACGGCCAGT GTGTCCAAGT GTCCATCACC
    AATGCCACCG GCCCCATGGA CAACAGCTTG GCAACCAGCA ACACTGCCAC CGAGAAATCC
    TTCCCTTTGC ACTCCCCAGC GGCTGGCGTG GCTCACCGAT TCCACAGTAC TTGCGGCAAG
    AATGTGGCCC TGGAGGAGGA TGGCACAAGG GCGGTACGTG CGGTGGGCTA TGCGCATGGC
    CTGGTCTTCA GCACCAAGGA ACTCAAGACG GAGGAAGTAT TTGAGGTGAA AGTAGAGGAA
    CTGGATGAGA AGTGGGCGGG CTCCCTCCGG CTGGGGCTGA CCACACTAGC GCCCGGGGAG
    ATGGGGCCTG GCGCAGGTGG GGGCCCAGGG CTGCCTCCCT CCCTCCCGGA GCTCCGGACA
    AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGTG ACGGGCAGCT CCAGAGGATG
    AACTACGGCC GGAGCCTGGA GAGGCTGGGG GTGGGGAGCC GCGTGGGCAT TCGTCGGGGC
    GCAGATGACA CGATGCACAT CCTGGTGGAT GGAGAGGACA TGGGGCCCGC AGCTACCGGC
    ATTGCCAAGA ATGTGTGGGC CGTGCTGGAT CTCTACGGGC CGGTACGGAG TGTGTCTGTG
    GTCAGCTCCA CAAGGCTGGA GGAGCCCGAG GGCACCCAGC CTCCATCTCC CAGCTCGGAC
    ACGGGCAGCG AGGCCGAGGA AGATGACGAT GGGGAGGAGC ACAGCTTGAG AGGTCAGAGT
    CAAGTGGCCA TGATGTCGAC GTCACTGGAG TTCCTGGAGA ACCATGGGAA GAATATCCTT
    TTGTCCAACG GGAACCGTAC GGCCACACGC GTAGCCAGCT ACAACCAGGG CATCGTTGTC
    ATCAACCAGC CCCTGGTGCC CCAGCTCCTA GTCCAGGTGC GGATAGACTT CCTGAACCGG
    CAGTGGACAT CTTCCCTGGT CCTGGGAGTC ATCACCTGCC CGCCTGAGAG GCTCAACTTC
    CCTGCTTCTG CCTGCGCCCT CAAACGGGCA GCCTGGCTGC TGCGGGGCCG GGGCATCTTC
    CACAATGGTC TCAAGATTTG TGAGAAGTTT GGGCCAAATC TGGACACGTG TCCTGAAGGC
    ACCATCCTGG GGCTGCGGCT AGACAGCTCT GGGGGCCTGC ATCTCCACGT CAATGGGATG
    GACCAGGGGG TGGCGGTATC AGATGTCCCC CAGCCCTGCC ATGCGCTTGT GGACCTCTAT
    GGGCAGTGTG AGCAGGTGAC AATCGTGAGT CCTGAACCAG GGGCAGCCAG TGGGAAGAGT
    GCTGGAACCC AAGGGGACAT GGAGAAAGCG GACATGGTGG ATGGTATCAA GGAAAGTGTG
    TGCTGGGGCC CCCTGCCAGC TGCCAGCCCT CTCAAGAGCT GCGAGTACCA CGCCCTCTGC
    TCCCGCTTCC AAGAACTGCT GTTGCTTCCT GAGGACTATT TCATGCCTCC ACCCAAGCGG
    AGCCTGTGCT ACTGTGAGTC TTGCCGGAAG CTTCGAGGTG ACGAGGCCCA CAGGCGCCGT
    GGGGAGCCCC CCCGGGAATA CGCGCTGCCC TTCGGCTGGT GCAGGTTCAA TCTCAGGGTG
    AATCCCCGCC TGGAAGCTGG AACGATGACT AAGAAGTGGC ACATGGCATA TCACGGGAGC
    AACGTGGCAG CCATCCGGAG GGTGCTAGAC CGAGGAGAGC TGGGAGCAGG CACCGCCTCC
    ATCCTGAGCT GCCGGCCCTT GAAAGGCAAG CCTGGGGTGG GGTTTGAGGA GCCTGGCGAG
    AACTGCGCCC CACCCCGGGA GGAGCAGCCC CCTTCCGTCT TGCTGTCCCC CTCCCTCCAG
    TATGCTGGGG CCGAGACCCT AGCGTCCAAA GTGCAATTCC GGGATCCCAA GTCCCAGCGA
    ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGCGTGCGCC CGGGCTCCTA CACCCCCGGA
    CCCCCTTCGG CCACCCTCCG AGAACCTCCC GACCCTCACT TCAGCCCCGC AGAACTCGAG
    TGGATAACCA AGGAGAAGGG GGCCACACTC CTCTATGCCC TGCTGGTGCG GGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968999.1
    CDS370..5049
    Translation

    Target ORF information:

    RefSeq Version XM_014113524.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGC CTTCATGGTG
    TCCCCAGCGC AGCCCCGGCC GGAGACGTTT CCTAACAGCC TTGAGTCGCA TAATGACTTT
    GCCAGCATGG AGCTCTCTGA GGTGGTGGGC AACGCCATCC TTTCTGCCTA CAACGGGGGG
    CTCCTAAATG TGAACCTGAG CTCCCCACCG GCAGGGGAAG GACTGGGGTC TGGCGGGGCT
    GCCACCTCGC CCATCCTCAC CTCCAACGAC GCCCTGCTCT TTCACGAGAA GTGTGGGACT
    CTCATCAAAC TCAGCAACAA CAATAAGACA GCTGAGCGCC GCCGGCCCCT GGACGAATTC
    AACAACGGGG TTGTCATGAC CAACCGCCCT CTCCGGGACA ATGAGATGTT TGAGATCCGC
    ATTGACAAGC TCGTAGACAA GTGGTCGGGC TCCATTGAGA TTGGTGTCAC CACCCACAAC
    CCCAACAACC TGGAGTACCC TGCCACCATG ACCAATTTAC AGTCAGGCAC CATCATGATG
    AGCGGCTGTG GGATCCTGAC CAATGGCAAG GGCACCCGCC GGGAGTACTG TGAATTCAGC
    CTGGATGAGC TGCAGGAGGG CGACCACATT GGTCTCACAA GGAAGTCCAA CTCAGCCCTG
    CACTTCTTTA TCAATGGCAT TGATCAGGGC GTGGCTACCC CACTGACACC CCCAGTGGTG
    TACGGCGTGG TGGACTTGTA CGGGATGGCT GTGAAGGTGA CCATCGTCCA CAATAACAAC
    CACAGCGACC GGCTGCGTCG AAACAATGCC ATCCTGCGGG CACTGTCCCC CGAGGGCGCC
    CTGCGTCGCG CCACTCCTGC CACCCAGGCA GAGCCCGAGC GCCTGCTCTT CCACCCTAAC
    TGCGGGCAGA AGGCAGCTAT CACCCACGAG GGACGCACTG CCCTGAGGCC CCATGCCACA
    GATGACTTCA ACCATGGCGT GGTGCTGAGT AGCAGAGCCC TGCGGGACGG AGAGGTGTTT
    CAGGTGCGCA TCGACAAGAT GGTGGACAAG TGGGCTGGCT CCATTGAGAT TGGTGTCACC
    ACCCACAACC CTGCCTACCT CCAGTTGCCC TCCACCATGA CCAACTTGCG CTCTGGGACC
    TGGATGATGA CCGGGAATGG GGTGATGCAC AACGGAACGA CCATTTTGGA CGAGTATGGG
    CACAATCTGG ACCGCCTCAA GGTGGGGGAG CAGGCAGGGG ACACTGTGGG CGTGGTGCGG
    CGGGAGGACG GGACCCTGCA CTTCTTCGTC AACGGGATGA CCCAGGGCCC TGCGGCCTGG
    AATGTGCCCC CTGGCGTCTA CGCTGTTGTG GACCTCTATG GCCAGGCAGC CCAAGCTACC
    ATTGTGGATG ACGTGGAGGT GCCCCCGGCC CCTGAGCCCC TCTCTGAAGG GAACAACCAG
    GTGTCTCCCA GCTCCCCATC ATCAGGGGCC GGGGGCTCTG ACCTGCGCTT CCATCAGCTG
    CACGGCAGTA ATGCAGTCAT CACCAACGGG GGCCGCACCG CACTCCGGCA CAACTGCCGC
    AGCGAGTTCA ATGACGCCAT CGTCATCTCC AACCGGGCCC TGCGGGACGG AGAGCTGTTT
    GAAATCGTTA TTCAGAAGAT GGTGGACCGC TGGTCAGGCT CCATTGAGGC TGGAGTGACT
    GCCATTCGGC CGGAAGACCT CGAATTCCCC AACACCATGA CAGACATTGA CTACGACACA
    TGGATGCTGA GTGGCACGGC CATCATGCAA GACGGGAACA CGATGCGCAA CAACTACGGG
    TGTGACCTGG ACGCCCTGGG CACGGGTGCA CGCATCGGCA TGATGCGAAC AGCCAAGGGT
    GACCTGCACT ACTTCATCAA CGGCCAGGAC CAAGGCGCCG CCTGCTCAGG CTTGCCTCCG
    GGTAAAGAGG TGTACGCAGT AGTGGATCTT TACGGCCAGT GTGTCCAAGT GTCCATCACC
    AATGCCACCG GCCCCATGGA CAACAGCTTG GCAACCAGCA ACACTGCCAC CGAGAAATCC
    TTCCCTTTGC ACTCCCCAGC GGCTGGCGTG GCTCACCGAT TCCACAGTAC TTGCGGCAAG
    AATGTGGCCC TGGAGGAGGA TGGCACAAGG GCGGTACGTG CGGTGGGCTA TGCGCATGGC
    CTGGTCTTCA GCACCAAGGA ACTCAAGACG GAGGAAGTAT TTGAGGTGAA AGTAGAGGAA
    CTGGATGAGA AGTGGGCGGG CTCCCTCCGG CTGGGGCTGA CCACACTAGC GCCCGGGGAG
    ATGGGGCCTG GCGCAGGTGG GGGCCCAGGG CTGCCTCCCT CCCTCCCGGA GCTCCGGACA
    AAGACCACCT GGATGGTGTC CAGCTGTGAA GTGAGGCGTG ACGGGCAGCT CCAGAGGATG
    AACTACGGCC GGAGCCTGGA GAGGCTGGGG GTGGGGAGCC GCGTGGGCAT TCGTCGGGGC
    GCAGATGACA CGATGCACAT CCTGGTGGAT GGAGAGGACA TGGGGCCCGC AGCTACCGGC
    ATTGCCAAGA ATGTGTGGGC CGTGCTGGAT CTCTACGGGC CGGTACGGAG TGTGTCTGTG
    GTCAGCTCCA CAAGGCTGGA GGAGCCCGAG GGCACCCAGC CTCCATCTCC CAGCTCGGAC
    ACGGGCAGCG AGGCCGAGGA AGATGACGAT GGGGAGGAGC ACAGCTTGAG AGGTCAGAGT
    CAAGTGGCCA TGATGTCGAC GTCACTGGAG TTCCTGGAGA ACCATGGGAA GAATATCCTT
    TTGTCCAACG GGAACCGTAC GGCCACACGC GTAGCCAGCT ACAACCAGGG CATCGTTGTC
    ATCAACCAGC CCCTGGTGCC CCAGCTCCTA GTCCAGGTGC GGATAGACTT CCTGAACCGG
    CAGTGGACAT CTTCCCTGGT CCTGGGAGTC ATCACCTGCC CGCCTGAGAG GCTCAACTTC
    CCTGCTTCTG CCTGCGCCCT CAAACGGGCA GCCTGGCTGC TGCGGGGCCG GGGCATCTTC
    CACAATGGTC TCAAGATTTG TGAGAAGTTT GGGCCAAATC TGGACACGTG TCCTGAAGGC
    ACCATCCTGG GGCTGCGGCT AGACAGCTCT GGGGGCCTGC ATCTCCACGT CAATGGGATG
    GACCAGGGGG TGGCGGTATC AGATGTCCCC CAGCCCTGCC ATGCGCTTGT GGACCTCTAT
    GGGCAGTGTG AGCAGGTGAC AATCGTGAGT CCTGAACCAG GGGCAGCCAG TGGGAAGAGT
    GCTGGAACCC AAGGGGACAT GGAGAAAGCG GACATGGTGG ATGGTATCAA GGAAAGTGTG
    TGCTGGGGCC CCCTGCCAGC TGCCAGCCCT CTCAAGAGCT GCGAGTACCA CGCCCTCTGC
    TCCCGCTTCC AAGAACTGCT GTTGCTTCCT GAGGACTATT TCATGCCTCC ACCCAAGCGG
    AGCCTGTGCT ACTGTGAGTC TTGCCGGAAG CTTCGAGGTG ACGAGGCCCA CAGGCGCCGT
    GGGGAGCCCC CCCGGGAATA CGCGCTGCCC TTCGGCTGGT GCAGGTTCAA TCTCAGGGTG
    AATCCCCGCC TGGAAGCTGG AACGATGACT AAGAAGTGGC ACATGGCATA TCACGGGAGC
    AACGTGGCAG CCATCCGGAG GGTGCTAGAC CGAGGAGAGC TGGGAGCAGG CACCGCCTCC
    ATCCTGAGCT GCCGGCCCTT GAAAGGCAAG CCTGGGGTGG GGTTTGAGGA GCCTGGCGAG
    AACTGCGCCC CACCCCGGGA GGAGCAGCCC CCTTCCGTCT TGCTGTCCCC CTCCCTCCAG
    TATGCTGGGG CCGAGACCCT AGCGTCCAAA GTGCAATTCC GGGATCCCAA GTCCCAGCGA
    ACGCACCAGG CCCAGGTGGC GTTCCAGGTG TGCGTGCGCC CGGGCTCCTA CACCCCCGGA
    CCCCCTTCGG CCACCCTCCG AGAACCTCCC GACCCTCACT TCAGCCCCGC AGAACTCGAG
    TGGATAACCA AGGAGAAGGG GGCCACACTC CTCTATGCCC TGCTGGTGCG GGTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36410
    Clone ID Related Accession (Same CDS sequence) XM_014113526.1
    Accession Version XM_014113526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4626bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neuralized-like protein 4 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGA CTTTGCCAGC
    ATGGAGCTCT CTGAGGTGGT GGGCAACGCC ATCCTTTCTG CCTACAACGG GGGGCTCCTA
    AATGTGAACC TGAGCTCCCC ACCGGCAGGG GAAGGACTGG GGTCTGGCGG GGCTGCCACC
    TCGCCCATCC TCACCTCCAA CGACGCCCTG CTCTTTCACG AGAAGTGTGG GACTCTCATC
    AAACTCAGCA ACAACAATAA GACAGCTGAG CGCCGCCGGC CCCTGGACGA ATTCAACAAC
    GGGGTTGTCA TGACCAACCG CCCTCTCCGG GACAATGAGA TGTTTGAGAT CCGCATTGAC
    AAGCTCGTAG ACAAGTGGTC GGGCTCCATT GAGATTGGTG TCACCACCCA CAACCCCAAC
    AACCTGGAGT ACCCTGCCAC CATGACCAAT TTACAGTCAG GCACCATCAT GATGAGCGGC
    TGTGGGATCC TGACCAATGG CAAGGGCACC CGCCGGGAGT ACTGTGAATT CAGCCTGGAT
    GAGCTGCAGG AGGGCGACCA CATTGGTCTC ACAAGGAAGT CCAACTCAGC CCTGCACTTC
    TTTATCAATG GCATTGATCA GGGCGTGGCT ACCCCACTGA CACCCCCAGT GGTGTACGGC
    GTGGTGGACT TGTACGGGAT GGCTGTGAAG GTGACCATCG TCCACAATAA CAACCACAGC
    GACCGGCTGC GTCGAAACAA TGCCATCCTG CGGGCACTGT CCCCCGAGGG CGCCCTGCGT
    CGCGCCACTC CTGCCACCCA GGCAGAGCCC GAGCGCCTGC TCTTCCACCC TAACTGCGGG
    CAGAAGGCAG CTATCACCCA CGAGGGACGC ACTGCCCTGA GGCCCCAGTA TGGCCCATGT
    GCCACAGATG ACTTCAACCA TGGCGTGGTG CTGAGTAGCA GAGCCCTGCG GGACGGAGAG
    GTGTTTCAGG TGCGCATCGA CAAGATGGTG GACAAGTGGG CTGGCTCCAT TGAGATTGGT
    GTCACCACCC ACAACCCTGC CTACCTCCAG TTGCCCTCCA CCATGACCAA CTTGCGCTCT
    GGGACCTGGA TGATGACCGG GAATGGGGTG ATGCACAACG GAACGACCAT TTTGGACGAG
    TATGGGCACA ATCTGGACCG CCTCAAGGTG GGGGAGCAGG CAGGGGACAC TGTGGGCGTG
    GTGCGGCGGG AGGACGGGAC CCTGCACTTC TTCGTCAACG GGATGACCCA GGGCCCTGCG
    GCCTGGAATG TGCCCCCTGG CGTCTACGCT GTTGTGGACC TCTATGGCCA GGCAGCCCAA
    GCTACCATTG TGGATGACGT GGAGGTGCCC CCGGCCCCTG AGCCCCTCTC TGAAGGGAAC
    AACCAGGTGT CTCCCAGCTC CCCATCATCA GGGGCCGGGG GCTCTGACCT GCGCTTCCAT
    CAGCTGCACG GCAGTAATGC AGTCATCACC AACGGGGGCC GCACCGCACT CCGGCACAAC
    TGCCGCAGCG AGTTCAATGA CGCCATCGTC ATCTCCAACC GGGCCCTGCG GGACGGAGAG
    CTGTTTGAAA TCGTTATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA
    GTGACTGCCA TTCGGCCGGA AGACCTCGAA TTCCCCAACA CCATGACAGA CATTGACTAC
    GACACATGGA TGCTGAGTGG CACGGCCATC ATGCAAGACG GGAACACGAT GCGCAACAAC
    TACGGGTGTG ACCTGGACGC CCTGGGCACG GGTGCACGCA TCGGCATGAT GCGAACAGCC
    AAGGGTGACC TGCACTACTT CATCAACGGC CAGGACCAAG GCGCCGCCTG CTCAGGCTTG
    CCTCCGGGTA AAGAGGTGTA CGCAGTAGTG GATCTTTACG GCCAGTGTGT CCAAGTGTCC
    ATCACCAATG CCACCGGCCC CATGGACAAC AGCTTGGCAA CCAGCAACAC TGCCACCGAG
    AAATCCTTCC CTTTGCACTC CCCAGCGGCT GGCGTGGCTC ACCGATTCCA CAGTACTTGC
    GGCAAGAATG TGGCCCTGGA GGAGGATGGC ACAAGGGCGG TACGTGCGGT GGGCTATGCG
    CATGGCCTGG TCTTCAGCAC CAAGGAACTC AAGACGGAGG AAGTATTTGA GGTGAAAGTA
    GAGGAACTGG ATGAGAAGTG GGCGGGCTCC CTCCGGCTGG GGCTGACCAC ACTAGCGCCC
    GGGGAGATGG GGCCTGGCGC AGGTGGGGGC CCAGGGCTGC CTCCCTCCCT CCCGGAGCTC
    CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGTGACGG GCAGCTCCAG
    AGGATGAACT ACGGCCGGAG CCTGGAGAGG CTGGGGGTGG GGAGCCGCGT GGGCATTCGT
    CGGGGCGCAG ATGACACGAT GCACATCCTG GTGGATGGAG AGGACATGGG GCCCGCAGCT
    ACCGGCATTG CCAAGAATGT GTGGGCCGTG CTGGATCTCT ACGGGCCGGT ACGGAGTGTG
    TCTGTGGTCA GCTCCACAAG GCTGGAGGAG CCCGAGGGCA CCCAGCCTCC ATCTCCCAGC
    TCGGACACGG GCAGCGAGGC CGAGGAAGAT GACGATGGGG AGGAGCACAG CTTGAGAGGT
    CAGAGTCAAG TGGCCATGAT GTCGACGTCA CTGGAGTTCC TGGAGAACCA TGGGAAGAAT
    ATCCTTTTGT CCAACGGGAA CCGTACGGCC ACACGCGTAG CCAGCTACAA CCAGGGCATC
    GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTCCTAGTCC AGGTGCGGAT AGACTTCCTG
    AACCGGCAGT GGACATCTTC CCTGGTCCTG GGAGTCATCA CCTGCCCGCC TGAGAGGCTC
    AACTTCCCTG CTTCTGCCTG CGCCCTCAAA CGGGCAGCCT GGCTGCTGCG GGGCCGGGGC
    ATCTTCCACA ATGGTCTCAA GATTTGTGAG AAGTTTGGGC CAAATCTGGA CACGTGTCCT
    GAAGGCACCA TCCTGGGGCT GCGGCTAGAC AGCTCTGGGG GCCTGCATCT CCACGTCAAT
    GGGATGGACC AGGGGGTGGC GGTATCAGAT GTCCCCCAGC CCTGCCATGC GCTTGTGGAC
    CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGTCCTG AACCAGGGGC AGCCAGTGGG
    AAGAGTGCTG GAACCCAAGG GGACATGGAG AAAGCGGACA TGGTGGATGG TATCAAGGAA
    AGTGTGTGCT GGGGCCCCCT GCCAGCTGCC AGCCCTCTCA AGAGCTGCGA GTACCACGCC
    CTCTGCTCCC GCTTCCAAGA ACTGCTGTTG CTTCCTGAGG ACTATTTCAT GCCTCCACCC
    AAGCGGAGCC TGTGCTACTG TGAGTCTTGC CGGAAGCTTC GAGGTGACGA GGCCCACAGG
    CGCCGTGGGG AGCCCCCCCG GGAATACGCG CTGCCCTTCG GCTGGTGCAG GTTCAATCTC
    AGGGTGAATC CCCGCCTGGA AGCTGGAACG ATGACTAAGA AGTGGCACAT GGCATATCAC
    GGGAGCAACG TGGCAGCCAT CCGGAGGGTG CTAGACCGAG GAGAGCTGGG AGCAGGCACC
    GCCTCCATCC TGAGCTGCCG GCCCTTGAAA GGCAAGCCTG GGGTGGGGTT TGAGGAGCCT
    GGCGAGAACT GCGCCCCACC CCGGGAGGAG CAGCCCCCTT CCGTCTTGCT GTCCCCCTCC
    CTCCAGTATG CTGGGGCCGA GACCCTAGCG TCCAAAGTGC AATTCCGGGA TCCCAAGTCC
    CAGCGAACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGCG TGCGCCCGGG CTCCTACACC
    CCCGGACCCC CTTCGGCCAC CCTCCGAGAA CCTCCCGACC CTCACTTCAG CCCCGCAGAA
    CTCGAGTGGA TAACCAAGGA GAAGGGGGCC ACACTCCTCT ATGCCCTGCT GGTGCGGGTG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969001.1
    CDS370..4995
    Translation

    Target ORF information:

    RefSeq Version XM_014113526.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris neuralized E3 ubiquitin protein ligase 4 (NEURL4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCGGCGG GGTCGGGTGG GAGCGGGGGC TCCGGGGGTG GCCCCGGGCC GGGGCCTGGC 
    GGGGGTGGGG GCCCCGGCGG GAGCGGCGCA GGGCCGGGGT CCGGCGGGGA GTTGCACCCC
    CGCACCGGGC GCCTGGTGAG CCTGTCGGCC TGCGGGCGTA CCGCGCGGCG GCAGCAGCCG
    GGCCAGGAGT TTAACCACGG GCTGGTGTTG AGCCGGGAGC CCTTGCGCGA TGGACGCGTC
    TTCACCGTCC GCATCGACCG CAAGGTCAAC TCCTGGAGTG GCTCCATTGA GATTGGGGTG
    ACGGCCCTAG ACCCCAGTGT TCTGGACTTC CCAAGCAGCG CCACAGGGCT GAAGGGGGGC
    TCATGGGTAG TGTCAGGCTG CTCGGTGTTG AGGGATGGAC GCTCTGTGCT GGAGGAGTAT
    GGTCAGGACC TGGACCAGCT TGGTGAAGGG GACCGTGTGG GCGTGGAGCG CACAGTTGCT
    GGGGAGCTGC GGCTCTGGGT GAATGGGCGG GACTGTGGTG TTGCCGCCAC GGGCCTCCCC
    GCCCGGGTCT GGGCCGTGGT GGACCTTTAC GGCAAGTGCA CCCAGATCAC CGTGCTGCCC
    CCCGAGCCAG GTTTCAGCCC CCCTGCTCCC ATCCCCACAC CTCCCCTCGA GCCCTCTGCA
    CCCCCTGAAG ATTCCACCTT GACTGAACAG GCGACCTCTG GGGATGAAGA CTTTGCCAGC
    ATGGAGCTCT CTGAGGTGGT GGGCAACGCC ATCCTTTCTG CCTACAACGG GGGGCTCCTA
    AATGTGAACC TGAGCTCCCC ACCGGCAGGG GAAGGACTGG GGTCTGGCGG GGCTGCCACC
    TCGCCCATCC TCACCTCCAA CGACGCCCTG CTCTTTCACG AGAAGTGTGG GACTCTCATC
    AAACTCAGCA ACAACAATAA GACAGCTGAG CGCCGCCGGC CCCTGGACGA ATTCAACAAC
    GGGGTTGTCA TGACCAACCG CCCTCTCCGG GACAATGAGA TGTTTGAGAT CCGCATTGAC
    AAGCTCGTAG ACAAGTGGTC GGGCTCCATT GAGATTGGTG TCACCACCCA CAACCCCAAC
    AACCTGGAGT ACCCTGCCAC CATGACCAAT TTACAGTCAG GCACCATCAT GATGAGCGGC
    TGTGGGATCC TGACCAATGG CAAGGGCACC CGCCGGGAGT ACTGTGAATT CAGCCTGGAT
    GAGCTGCAGG AGGGCGACCA CATTGGTCTC ACAAGGAAGT CCAACTCAGC CCTGCACTTC
    TTTATCAATG GCATTGATCA GGGCGTGGCT ACCCCACTGA CACCCCCAGT GGTGTACGGC
    GTGGTGGACT TGTACGGGAT GGCTGTGAAG GTGACCATCG TCCACAATAA CAACCACAGC
    GACCGGCTGC GTCGAAACAA TGCCATCCTG CGGGCACTGT CCCCCGAGGG CGCCCTGCGT
    CGCGCCACTC CTGCCACCCA GGCAGAGCCC GAGCGCCTGC TCTTCCACCC TAACTGCGGG
    CAGAAGGCAG CTATCACCCA CGAGGGACGC ACTGCCCTGA GGCCCCAGTA TGGCCCATGT
    GCCACAGATG ACTTCAACCA TGGCGTGGTG CTGAGTAGCA GAGCCCTGCG GGACGGAGAG
    GTGTTTCAGG TGCGCATCGA CAAGATGGTG GACAAGTGGG CTGGCTCCAT TGAGATTGGT
    GTCACCACCC ACAACCCTGC CTACCTCCAG TTGCCCTCCA CCATGACCAA CTTGCGCTCT
    GGGACCTGGA TGATGACCGG GAATGGGGTG ATGCACAACG GAACGACCAT TTTGGACGAG
    TATGGGCACA ATCTGGACCG CCTCAAGGTG GGGGAGCAGG CAGGGGACAC TGTGGGCGTG
    GTGCGGCGGG AGGACGGGAC CCTGCACTTC TTCGTCAACG GGATGACCCA GGGCCCTGCG
    GCCTGGAATG TGCCCCCTGG CGTCTACGCT GTTGTGGACC TCTATGGCCA GGCAGCCCAA
    GCTACCATTG TGGATGACGT GGAGGTGCCC CCGGCCCCTG AGCCCCTCTC TGAAGGGAAC
    AACCAGGTGT CTCCCAGCTC CCCATCATCA GGGGCCGGGG GCTCTGACCT GCGCTTCCAT
    CAGCTGCACG GCAGTAATGC AGTCATCACC AACGGGGGCC GCACCGCACT CCGGCACAAC
    TGCCGCAGCG AGTTCAATGA CGCCATCGTC ATCTCCAACC GGGCCCTGCG GGACGGAGAG
    CTGTTTGAAA TCGTTATTCA GAAGATGGTG GACCGCTGGT CAGGCTCCAT TGAGGCTGGA
    GTGACTGCCA TTCGGCCGGA AGACCTCGAA TTCCCCAACA CCATGACAGA CATTGACTAC
    GACACATGGA TGCTGAGTGG CACGGCCATC ATGCAAGACG GGAACACGAT GCGCAACAAC
    TACGGGTGTG ACCTGGACGC CCTGGGCACG GGTGCACGCA TCGGCATGAT GCGAACAGCC
    AAGGGTGACC TGCACTACTT CATCAACGGC CAGGACCAAG GCGCCGCCTG CTCAGGCTTG
    CCTCCGGGTA AAGAGGTGTA CGCAGTAGTG GATCTTTACG GCCAGTGTGT CCAAGTGTCC
    ATCACCAATG CCACCGGCCC CATGGACAAC AGCTTGGCAA CCAGCAACAC TGCCACCGAG
    AAATCCTTCC CTTTGCACTC CCCAGCGGCT GGCGTGGCTC ACCGATTCCA CAGTACTTGC
    GGCAAGAATG TGGCCCTGGA GGAGGATGGC ACAAGGGCGG TACGTGCGGT GGGCTATGCG
    CATGGCCTGG TCTTCAGCAC CAAGGAACTC AAGACGGAGG AAGTATTTGA GGTGAAAGTA
    GAGGAACTGG ATGAGAAGTG GGCGGGCTCC CTCCGGCTGG GGCTGACCAC ACTAGCGCCC
    GGGGAGATGG GGCCTGGCGC AGGTGGGGGC CCAGGGCTGC CTCCCTCCCT CCCGGAGCTC
    CGGACAAAGA CCACCTGGAT GGTGTCCAGC TGTGAAGTGA GGCGTGACGG GCAGCTCCAG
    AGGATGAACT ACGGCCGGAG CCTGGAGAGG CTGGGGGTGG GGAGCCGCGT GGGCATTCGT
    CGGGGCGCAG ATGACACGAT GCACATCCTG GTGGATGGAG AGGACATGGG GCCCGCAGCT
    ACCGGCATTG CCAAGAATGT GTGGGCCGTG CTGGATCTCT ACGGGCCGGT ACGGAGTGTG
    TCTGTGGTCA GCTCCACAAG GCTGGAGGAG CCCGAGGGCA CCCAGCCTCC ATCTCCCAGC
    TCGGACACGG GCAGCGAGGC CGAGGAAGAT GACGATGGGG AGGAGCACAG CTTGAGAGGT
    CAGAGTCAAG TGGCCATGAT GTCGACGTCA CTGGAGTTCC TGGAGAACCA TGGGAAGAAT
    ATCCTTTTGT CCAACGGGAA CCGTACGGCC ACACGCGTAG CCAGCTACAA CCAGGGCATC
    GTTGTCATCA ACCAGCCCCT GGTGCCCCAG CTCCTAGTCC AGGTGCGGAT AGACTTCCTG
    AACCGGCAGT GGACATCTTC CCTGGTCCTG GGAGTCATCA CCTGCCCGCC TGAGAGGCTC
    AACTTCCCTG CTTCTGCCTG CGCCCTCAAA CGGGCAGCCT GGCTGCTGCG GGGCCGGGGC
    ATCTTCCACA ATGGTCTCAA GATTTGTGAG AAGTTTGGGC CAAATCTGGA CACGTGTCCT
    GAAGGCACCA TCCTGGGGCT GCGGCTAGAC AGCTCTGGGG GCCTGCATCT CCACGTCAAT
    GGGATGGACC AGGGGGTGGC GGTATCAGAT GTCCCCCAGC CCTGCCATGC GCTTGTGGAC
    CTCTATGGGC AGTGTGAGCA GGTGACAATC GTGAGTCCTG AACCAGGGGC AGCCAGTGGG
    AAGAGTGCTG GAACCCAAGG GGACATGGAG AAAGCGGACA TGGTGGATGG TATCAAGGAA
    AGTGTGTGCT GGGGCCCCCT GCCAGCTGCC AGCCCTCTCA AGAGCTGCGA GTACCACGCC
    CTCTGCTCCC GCTTCCAAGA ACTGCTGTTG CTTCCTGAGG ACTATTTCAT GCCTCCACCC
    AAGCGGAGCC TGTGCTACTG TGAGTCTTGC CGGAAGCTTC GAGGTGACGA GGCCCACAGG
    CGCCGTGGGG AGCCCCCCCG GGAATACGCG CTGCCCTTCG GCTGGTGCAG GTTCAATCTC
    AGGGTGAATC CCCGCCTGGA AGCTGGAACG ATGACTAAGA AGTGGCACAT GGCATATCAC
    GGGAGCAACG TGGCAGCCAT CCGGAGGGTG CTAGACCGAG GAGAGCTGGG AGCAGGCACC
    GCCTCCATCC TGAGCTGCCG GCCCTTGAAA GGCAAGCCTG GGGTGGGGTT TGAGGAGCCT
    GGCGAGAACT GCGCCCCACC CCGGGAGGAG CAGCCCCCTT CCGTCTTGCT GTCCCCCTCC
    CTCCAGTATG CTGGGGCCGA GACCCTAGCG TCCAAAGTGC AATTCCGGGA TCCCAAGTCC
    CAGCGAACGC ACCAGGCCCA GGTGGCGTTC CAGGTGTGCG TGCGCCCGGG CTCCTACACC
    CCCGGACCCC CTTCGGCCAC CCTCCGAGAA CCTCCCGACC CTCACTTCAG CCCCGCAGAA
    CTCGAGTGGA TAACCAAGGA GAAGGGGGCC ACACTCCTCT ATGCCCTGCT GGTGCGGGTG
    GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.