NLRP1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following NLRP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe06119 XM_005619883.3
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe06116 XM_005619880.3
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe06117 XM_005619881.3
Latest version!
Canis lupus familiaris NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe06118 XM_005619882.2
Latest version!
Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe06116 XM_005619880.2 Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe06117 XM_005619881.2 Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe06119 XM_005619883.2 Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe06119
    Clone ID Related Accession (Same CDS sequence) XM_005619883.3 , XM_005619883.2
    Accession Version XM_005619883.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619883.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTGACGCCAC AGATGAAAAG CAATGGAAAC CACCGGGAAC CCGAATCAGC
    TCCATGAAGA CTTCAGATGT CGATATCTCT AGACACAGAA CCATCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619940.1
    CDS1807..5277
    Translation

    Target ORF information:

    RefSeq Version XM_005619883.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619883.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTGACGCCAC AGATGAAAAG CAATGGAAAC CACCGGGAAC CCGAATCAGC
    TCCATGAAGA CTTCAGATGT CGATATCTCT AGACACAGAA CCATCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06119
    Clone ID Related Accession (Same CDS sequence) XM_005619883.3 , XM_005619883.2
    Accession Version XM_005619883.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTGACGCCAC AGATGAAAAG CAATGGAAAC CACCGGGAAC CCGAATCAGC
    TCCATGAAGA CTTCAGATGT CGATATCTCT AGACACAGAA CCATCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619940.1
    CDS245..3715
    Translation

    Target ORF information:

    RefSeq Version XM_005619883.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTGACGCCAC AGATGAAAAG CAATGGAAAC CACCGGGAAC CCGAATCAGC
    TCCATGAAGA CTTCAGATGT CGATATCTCT AGACACAGAA CCATCAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06116
    Clone ID Related Accession (Same CDS sequence) XM_005619880.3 , XM_005619880.2
    Accession Version XM_005619880.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTAGCAGCGT GGACACCTCC CTGTTCTACG CGGCCCACTT AAAAGATGAG
    GGGATGCTGG TGGAGACGCC AGCCAGGGTG GAGGCAGACC ACGTAGTCCT GGAGAACCCC
    AGCTTCTCCC CCATAGGAGT CCTGCTGAGG ACCATCCACG CCGCCCTGCG CTTCCTCCCC
    GTCACCTCTG TTGTGCTGCT GTACCATCAC CTGAGGCCCG GGGAAGTCGC CTTCCACCTA
    TACCTCATCC CCAGTGACTA CTCCATCCGC AAGGCCATAG ATAATGAAGA GAAAAAGTTC
    CAGTTTGTGC AGATACACAA GCCGCCCCCA CTGGCCTCGC TCTACATGGG CTCCCGCTAC
    ACTGTGTCTG GCTCAGAGAA GCTAGAGATA ATCCCCAAGG AGCTGGAGCT CTGCTACCGC
    AGCCCCAGGG AACCCCAGCT CTTCTCTGAG TTCTACGTCA GCCACTTGGG GTCGGGGATC
    AGGCTGCAAG TGAGAGACAA AAAGGATGAC ACAGTGGTGT GGGAAGCCCT GGTGAAACCA
    GGGGATCTCC GGCCTGTGGC TTCTCTGGCT CCTCCAGCCC CCGCAGATGC CCCGGCCTGG
    CTGCACTTCG TGGACCGACA TCGGGAGCAA CTGGTGGCTC GGGTGACGTC GGTGGATCCT
    GTGCTGGACA TGCTGCATGG GCAGGTGTTG AGCGAGGAGC AGTACGAGAG GGTGCGGGCC
    GAGGCCACGA CCCCCAGCCA GATGCGGAAG CTGTTCAGTT TCAGCAGGTC GTGGGACCGG
    GCCTGCAAGG ACCAGGTGTA CCAGGCTCTG AAGGAGGCCC ACCCTCACCT CATCATGGAA
    CTCTGGGAGA AGTGGGGCGG CAACAGTGGC AAGCAGGCGC CTCCTGGCCA GCCCAGCAGC
    TGCATCTCCA GCACCTGCCA TGAGGCCTGG CTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619937.1
    CDS1807..6105
    Translation

    Target ORF information:

    RefSeq Version XM_005619880.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619880.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTAGCAGCGT GGACACCTCC CTGTTCTACG CGGCCCACTT AAAAGATGAG
    GGGATGCTGG TGGAGACGCC AGCCAGGGTG GAGGCAGACC ACGTAGTCCT GGAGAACCCC
    AGCTTCTCCC CCATAGGAGT CCTGCTGAGG ACCATCCACG CCGCCCTGCG CTTCCTCCCC
    GTCACCTCTG TTGTGCTGCT GTACCATCAC CTGAGGCCCG GGGAAGTCGC CTTCCACCTA
    TACCTCATCC CCAGTGACTA CTCCATCCGC AAGGCCATAG ATAATGAAGA GAAAAAGTTC
    CAGTTTGTGC AGATACACAA GCCGCCCCCA CTGGCCTCGC TCTACATGGG CTCCCGCTAC
    ACTGTGTCTG GCTCAGAGAA GCTAGAGATA ATCCCCAAGG AGCTGGAGCT CTGCTACCGC
    AGCCCCAGGG AACCCCAGCT CTTCTCTGAG TTCTACGTCA GCCACTTGGG GTCGGGGATC
    AGGCTGCAAG TGAGAGACAA AAAGGATGAC ACAGTGGTGT GGGAAGCCCT GGTGAAACCA
    GGGGATCTCC GGCCTGTGGC TTCTCTGGCT CCTCCAGCCC CCGCAGATGC CCCGGCCTGG
    CTGCACTTCG TGGACCGACA TCGGGAGCAA CTGGTGGCTC GGGTGACGTC GGTGGATCCT
    GTGCTGGACA TGCTGCATGG GCAGGTGTTG AGCGAGGAGC AGTACGAGAG GGTGCGGGCC
    GAGGCCACGA CCCCCAGCCA GATGCGGAAG CTGTTCAGTT TCAGCAGGTC GTGGGACCGG
    GCCTGCAAGG ACCAGGTGTA CCAGGCTCTG AAGGAGGCCC ACCCTCACCT CATCATGGAA
    CTCTGGGAGA AGTGGGGCGG CAACAGTGGC AAGCAGGCGC CTCCTGGCCA GCCCAGCAGC
    TGCATCTCCA GCACCTGCCA TGAGGCCTGG CTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06116
    Clone ID Related Accession (Same CDS sequence) XM_005619880.3 , XM_005619880.2
    Accession Version XM_005619880.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTAGCAGCGT GGACACCTCC CTGTTCTACG CGGCCCACTT AAAAGATGAG
    GGGATGCTGG TGGAGACGCC AGCCAGGGTG GAGGCAGACC ACGTAGTCCT GGAGAACCCC
    AGCTTCTCCC CCATAGGAGT CCTGCTGAGG ACCATCCACG CCGCCCTGCG CTTCCTCCCC
    GTCACCTCTG TTGTGCTGCT GTACCATCAC CTGAGGCCCG GGGAAGTCGC CTTCCACCTA
    TACCTCATCC CCAGTGACTA CTCCATCCGC AAGGCCATAG ATAATGAAGA GAAAAAGTTC
    CAGTTTGTGC AGATACACAA GCCGCCCCCA CTGGCCTCGC TCTACATGGG CTCCCGCTAC
    ACTGTGTCTG GCTCAGAGAA GCTAGAGATA ATCCCCAAGG AGCTGGAGCT CTGCTACCGC
    AGCCCCAGGG AACCCCAGCT CTTCTCTGAG TTCTACGTCA GCCACTTGGG GTCGGGGATC
    AGGCTGCAAG TGAGAGACAA AAAGGATGAC ACAGTGGTGT GGGAAGCCCT GGTGAAACCA
    GGGGATCTCC GGCCTGTGGC TTCTCTGGCT CCTCCAGCCC CCGCAGATGC CCCGGCCTGG
    CTGCACTTCG TGGACCGACA TCGGGAGCAA CTGGTGGCTC GGGTGACGTC GGTGGATCCT
    GTGCTGGACA TGCTGCATGG GCAGGTGTTG AGCGAGGAGC AGTACGAGAG GGTGCGGGCC
    GAGGCCACGA CCCCCAGCCA GATGCGGAAG CTGTTCAGTT TCAGCAGGTC GTGGGACCGG
    GCCTGCAAGG ACCAGGTGTA CCAGGCTCTG AAGGAGGCCC ACCCTCACCT CATCATGGAA
    CTCTGGGAGA AGTGGGGCGG CAACAGTGGC AAGCAGGCGC CTCCTGGCCA GCCCAGCAGC
    TGCATCTCCA GCACCTGCCA TGAGGCCTGG CTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619937.1
    CDS243..4541
    Translation

    Target ORF information:

    RefSeq Version XM_005619880.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGCA
    GGAATCACAG AAAGGTATCA GCACCAGAGG AGAAAGAAGC CGTACCCCAC ATGGTCGTGG
    GAAGACAAGG ACCTGCACCA GATATTCATA CAGCTGCTGC TTCTGCATCA GCCTTACCCT
    AGAGGCCACG AGTCCTTAAC CAAGGGAAGA TGGCACCATG GGGCAGAGAA GCAAGGACAT
    TTGATTGAGG TCGAAGACCT ATTTGGCCCA GGCCTAGGTG CCCAGGAAGA GCCCCAAACA
    GTCATACTGC ACGGGGTTGC AGGCATCGGG AAGTCAACAC TGGCCAGGCA GATAAGGACG
    GCGTGGAAGG AAGGCCAGCT CTACAAGGAC CGCTTCCGAC ATGTCTTCTA CTTCAACTGC
    AGAGAGCTGG CCCGGTCCGA GACCATGAGC CTCACTGAGC TCATCACCAA GGCCCAGGCC
    GGCCCTGTAC TTCCCGCTGA ACAGATCCTG TCTCAGCCAG GGCGGCTGCT CTTCATCCTC
    GATGATTTGG ATGAGCCAAG ATGGGTCTTA GGGGAGCAGG AGTCAGAGCT GCATTCGTCC
    TGGAGCAAGC AGCGGCCGGT GCACGCACTG CTGGGCAGCT TGCTGGGCAA AACTGTGCTT
    CCCGAGGTGT CCCTGCTGGT TACAGTGCGG ACCACAGCTC TGCGGAGGCT TGTTCCCTCT
    GTGGGGCAGC TATGTTGGGT GGAGGTCCTG GGGTTCTCCG AGTCCGGCAG GAGGGAGTAC
    TTCTACAAGT ATTTCAAGAA CAAAAGCCAA GCCACCCAAG CCTTCAGCTT GGTTGAGTTA
    AACCCAACGC TGTTAACCAT GTGCCTCGTG CCCTTGGTGT CCTGGCTGCT GTGCACCTGC
    CTGAAGAAGC AGATGCAGCG AGGAGAGGAG CTCTCACTGG GTTCATCCCA GACCACCACG
    GCCCTCTGGC TATACTACCT CTCCCAGGCT CTCCCAGCCC AACTCCTTGG GGCGCAGCTG
    AGGGGCTTCT GCTCCCTGGC CGCTGAGGGC TTCTGGCAGG GAACGAACCT GCTCAGCAGG
    AGGGACCTCA GGAAGCACGG GTTCGATGAG GCCACCATCT CCACTTTCTT AAAGACAGGC
    ATCCTCCGAA AGCATCCCTC CCCTCTGAAC TACCACCTGG CTCACCGCTG TCTCCAGGAG
    TTCTTTGCAG CCGTGTCCTG CGTGCTGGGG GGTGAGGATG GCGGAAGCCC TCACCCCGAC
    AGCATCAGAG GGGCAGAGAA ACTGCTAGAG GTGTATGAGG GACATGACCT GTTTGGGGCC
    CCAACCACGC ATTTCCTCTT TGGCCTTCTG AGTGAGCAAG GGGAGCGAGA GATGGAGGCG
    GTGTTCCAGT GCAAGCTGCC CCGGGAGAGG AAGCGGGACC TGCTGCGCTG GGCTGAGGCG
    GGGCTCTGGC CTCGGCTCTC CGCACCTCAG CCATGCTCTC TGCACTTGCT CCACTGCCTG
    TACGAGATTC AGGACGAGGA CTTGCTGACA CGGGCCATGG CCCATTTCCA TGGAAGGAGC
    ATGTGTGTCC AAACTGGCGT GGAGTTCCTG GTGTGCACTT TCTGCTTTAA ATTCTGCTCC
    CACGTGAAAA GGCTTCAACT GAATGAGAGC CGGTGGGATG GACAAGCATG GAGGCCCTCC
    AGTGTGGCTC TGTTCAGCTG GGTCCCAGTC ACAGATGCCT GCTGGCAGGC TCTCTTTTCC
    ACTCTCCGGG CCTCTGGAAG CCTGAAGGAG CTGGACCTGA GTGGAAATCA CCTGAGCCAC
    CCCGCAGTTC AGAGTCTTTG TAAAGTCCTG AAATTCCCTT GCTGCCACTT GGAGACTGTG
    CGGTTGGCCG GCTGCGGCCT GACGGCGGAA GGCTGCAGCG ACCTGGCCTC TGCGCTGAGT
    GCCAGCCCGG CCCTGGCGGA GCTGGAGCTG AGTTTCAACT TGCTCCTGGA CGCTGGAGCC
    CAGCATCTGA GCAGGGGGCT GAGGCAGCCG GCCTGCAGGC TCCGCCGGCT GCTGCTGGCT
    GGCTGCAGCC TCACCTCCCG CTGCTGCGGG GCCCTGGCCT CCGCGCTCAG CACCAGCCCC
    CTCCTCACGG AACTAGACCT GCAGCAGAAC GAGCTGGGCG ACGTCGGCGT GCGGCTGCTG
    TGCGGAGGCC TTGGGCACCC CACCTGCCAG CTCACGCTGC TGTGGCTGGA CCAGACCCAG
    TTAAGTGAGG AGATGACACA GATGCTGAGG GCCCTGCAGG AGGAGAAGCC TCAGCTGTTC
    GTCTGCAGCA AATGGAAGCC AAGGGCGACC GTCCCCACCG AGGGCCTGAG TCGGGGAGAG
    ACAAGTGGCA CGTCCTCACT CAAGCAGCAG AGACAAGGAT CAGAGAGAAG CTGCCCTCAG
    GTAGGCCAGG TGCAGCTGGG TCGCCCACCC TCTCCTGCTG CCCCGTGGGA CCTGAACACA
    GAGCCTCTGA GGATCGGGGA TGGCTTCTGG GGCCCCCTGG GGCCTGTGGC TCCCGAGTGG
    CTGGCCGAGG ATGGAAGCCT CTACAGGGTT CACCTGCCTG TGGCCGGCTC CTACCACTGG
    CCCGACACAG GCCTCCGTTT TGAGGTGAGA GGACCAGCAA CCATTGACAT TGAATTCTGC
    GTCTGGGACC AGCACCTGCC CAGGGCCGGC CTATGGCACA GCTGGATGGT GGCGGGGCCT
    CTGTTTGACA TCAAGGCTGA GCCAGGGGCC GTGGCTACTG TGTGCCTCCC TCACTTCGTG
    GACCTGCAAG GTAGCAGCGT GGACACCTCC CTGTTCTACG CGGCCCACTT AAAAGATGAG
    GGGATGCTGG TGGAGACGCC AGCCAGGGTG GAGGCAGACC ACGTAGTCCT GGAGAACCCC
    AGCTTCTCCC CCATAGGAGT CCTGCTGAGG ACCATCCACG CCGCCCTGCG CTTCCTCCCC
    GTCACCTCTG TTGTGCTGCT GTACCATCAC CTGAGGCCCG GGGAAGTCGC CTTCCACCTA
    TACCTCATCC CCAGTGACTA CTCCATCCGC AAGGCCATAG ATAATGAAGA GAAAAAGTTC
    CAGTTTGTGC AGATACACAA GCCGCCCCCA CTGGCCTCGC TCTACATGGG CTCCCGCTAC
    ACTGTGTCTG GCTCAGAGAA GCTAGAGATA ATCCCCAAGG AGCTGGAGCT CTGCTACCGC
    AGCCCCAGGG AACCCCAGCT CTTCTCTGAG TTCTACGTCA GCCACTTGGG GTCGGGGATC
    AGGCTGCAAG TGAGAGACAA AAAGGATGAC ACAGTGGTGT GGGAAGCCCT GGTGAAACCA
    GGGGATCTCC GGCCTGTGGC TTCTCTGGCT CCTCCAGCCC CCGCAGATGC CCCGGCCTGG
    CTGCACTTCG TGGACCGACA TCGGGAGCAA CTGGTGGCTC GGGTGACGTC GGTGGATCCT
    GTGCTGGACA TGCTGCATGG GCAGGTGTTG AGCGAGGAGC AGTACGAGAG GGTGCGGGCC
    GAGGCCACGA CCCCCAGCCA GATGCGGAAG CTGTTCAGTT TCAGCAGGTC GTGGGACCGG
    GCCTGCAAGG ACCAGGTGTA CCAGGCTCTG AAGGAGGCCC ACCCTCACCT CATCATGGAA
    CTCTGGGAGA AGTGGGGCGG CAACAGTGGC AAGCAGGCGC CTCCTGGCCA GCCCAGCAGC
    TGCATCTCCA GCACCTGCCA TGAGGCCTGG CTCTGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06117
    Clone ID Related Accession (Same CDS sequence) XM_005619881.2 , XM_005619881.3
    Accession Version XM_005619881.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619881.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGGA
    ATCACAGAAA GGTATCAGCA CCAGAGGAGA AAGAAGCCGT ACCCCACATG GTCGTGGGAA
    GACAAGGACC TGCACCAGAT ATTCATACAG CTGCTGCTTC TGCATCAGCC TTACCCTAGA
    GGCCACGAGT CCTTAACCAA GGGAAGATGG CACCATGGGG CAGAGAAGCA AGGACATTTG
    ATTGAGGTCG AAGACCTATT TGGCCCAGGC CTAGGTGCCC AGGAAGAGCC CCAAACAGTC
    ATACTGCACG GGGTTGCAGG CATCGGGAAG TCAACACTGG CCAGGCAGAT AAGGACGGCG
    TGGAAGGAAG GCCAGCTCTA CAAGGACCGC TTCCGACATG TCTTCTACTT CAACTGCAGA
    GAGCTGGCCC GGTCCGAGAC CATGAGCCTC ACTGAGCTCA TCACCAAGGC CCAGGCCGGC
    CCTGTACTTC CCGCTGAACA GATCCTGTCT CAGCCAGGGC GGCTGCTCTT CATCCTCGAT
    GATTTGGATG AGCCAAGATG GGTCTTAGGG GAGCAGGAGT CAGAGCTGCA TTCGTCCTGG
    AGCAAGCAGC GGCCGGTGCA CGCACTGCTG GGCAGCTTGC TGGGCAAAAC TGTGCTTCCC
    GAGGTGTCCC TGCTGGTTAC AGTGCGGACC ACAGCTCTGC GGAGGCTTGT TCCCTCTGTG
    GGGCAGCTAT GTTGGGTGGA GGTCCTGGGG TTCTCCGAGT CCGGCAGGAG GGAGTACTTC
    TACAAGTATT TCAAGAACAA AAGCCAAGCC ACCCAAGCCT TCAGCTTGGT TGAGTTAAAC
    CCAACGCTGT TAACCATGTG CCTCGTGCCC TTGGTGTCCT GGCTGCTGTG CACCTGCCTG
    AAGAAGCAGA TGCAGCGAGG AGAGGAGCTC TCACTGGGTT CATCCCAGAC CACCACGGCC
    CTCTGGCTAT ACTACCTCTC CCAGGCTCTC CCAGCCCAAC TCCTTGGGGC GCAGCTGAGG
    GGCTTCTGCT CCCTGGCCGC TGAGGGCTTC TGGCAGGGAA CGAACCTGCT CAGCAGGAGG
    GACCTCAGGA AGCACGGGTT CGATGAGGCC ACCATCTCCA CTTTCTTAAA GACAGGCATC
    CTCCGAAAGC ATCCCTCCCC TCTGAACTAC CACCTGGCTC ACCGCTGTCT CCAGGAGTTC
    TTTGCAGCCG TGTCCTGCGT GCTGGGGGGT GAGGATGGCG GAAGCCCTCA CCCCGACAGC
    ATCAGAGGGG CAGAGAAACT GCTAGAGGTG TATGAGGGAC ATGACCTGTT TGGGGCCCCA
    ACCACGCATT TCCTCTTTGG CCTTCTGAGT GAGCAAGGGG AGCGAGAGAT GGAGGCGGTG
    TTCCAGTGCA AGCTGCCCCG GGAGAGGAAG CGGGACCTGC TGCGCTGGGC TGAGGCGGGG
    CTCTGGCCTC GGCTCTCCGC ACCTCAGCCA TGCTCTCTGC ACTTGCTCCA CTGCCTGTAC
    GAGATTCAGG ACGAGGACTT GCTGACACGG GCCATGGCCC ATTTCCATGG AAGGAGCATG
    TGTGTCCAAA CTGGCGTGGA GTTCCTGGTG TGCACTTTCT GCTTTAAATT CTGCTCCCAC
    GTGAAAAGGC TTCAACTGAA TGAGAGCCGG TGGGATGGAC AAGCATGGAG GCCCTCCAGT
    GTGGCTCTGT TCAGCTGGGT CCCAGTCACA GATGCCTGCT GGCAGGCTCT CTTTTCCACT
    CTCCGGGCCT CTGGAAGCCT GAAGGAGCTG GACCTGAGTG GAAATCACCT GAGCCACCCC
    GCAGTTCAGA GTCTTTGTAA AGTCCTGAAA TTCCCTTGCT GCCACTTGGA GACTGTGCGG
    TTGGCCGGCT GCGGCCTGAC GGCGGAAGGC TGCAGCGACC TGGCCTCTGC GCTGAGTGCC
    AGCCCGGCCC TGGCGGAGCT GGAGCTGAGT TTCAACTTGC TCCTGGACGC TGGAGCCCAG
    CATCTGAGCA GGGGGCTGAG GCAGCCGGCC TGCAGGCTCC GCCGGCTGCT GCTGGCTGGC
    TGCAGCCTCA CCTCCCGCTG CTGCGGGGCC CTGGCCTCCG CGCTCAGCAC CAGCCCCCTC
    CTCACGGAAC TAGACCTGCA GCAGAACGAG CTGGGCGACG TCGGCGTGCG GCTGCTGTGC
    GGAGGCCTTG GGCACCCCAC CTGCCAGCTC ACGCTGCTGT GGCTGGACCA GACCCAGTTA
    AGTGAGGAGA TGACACAGAT GCTGAGGGCC CTGCAGGAGG AGAAGCCTCA GCTGTTCGTC
    TGCAGCAAAT GGAAGCCAAG GGCGACCGTC CCCACCGAGG GCCTGAGTCG GGGAGAGACA
    AGTGGCACGT CCTCACTCAA GCAGCAGAGA CAAGGATCAG AGAGAAGCTG CCCTCAGGTA
    GGCCAGGTGC AGCTGGGTCG CCCACCCTCT CCTGCTGCCC CGTGGGACCT GAACACAGAG
    CCTCTGAGGA TCGGGGATGG CTTCTGGGGC CCCCTGGGGC CTGTGGCTCC CGAGTGGCTG
    GCCGAGGATG GAAGCCTCTA CAGGGTTCAC CTGCCTGTGG CCGGCTCCTA CCACTGGCCC
    GACACAGGCC TCCGTTTTGA GGTGAGAGGA CCAGCAACCA TTGACATTGA ATTCTGCGTC
    TGGGACCAGC ACCTGCCCAG GGCCGGCCTA TGGCACAGCT GGATGGTGGC GGGGCCTCTG
    TTTGACATCA AGGCTGAGCC AGGGGCCGTG GCTACTGTGT GCCTCCCTCA CTTCGTGGAC
    CTGCAAGGTA GCAGCGTGGA CACCTCCCTG TTCTACGCGG CCCACTTAAA AGATGAGGGG
    ATGCTGGTGG AGACGCCAGC CAGGGTGGAG GCAGACCACG TAGTCCTGGA GAACCCCAGC
    TTCTCCCCCA TAGGAGTCCT GCTGAGGACC ATCCACGCCG CCCTGCGCTT CCTCCCCGTC
    ACCTCTGTTG TGCTGCTGTA CCATCACCTG AGGCCCGGGG AAGTCGCCTT CCACCTATAC
    CTCATCCCCA GTGACTACTC CATCCGCAAG GCCATAGATA ATGAAGAGAA AAAGTTCCAG
    TTTGTGCAGA TACACAAGCC GCCCCCACTG GCCTCGCTCT ACATGGGCTC CCGCTACACT
    GTGTCTGGCT CAGAGAAGCT AGAGATAATC CCCAAGGAGC TGGAGCTCTG CTACCGCAGC
    CCCAGGGAAC CCCAGCTCTT CTCTGAGTTC TACGTCAGCC ACTTGGGGTC GGGGATCAGG
    CTGCAAGTGA GAGACAAAAA GGATGACACA GTGGTGTGGG AAGCCCTGGT GAAACCAGGG
    GATCTCCGGC CTGTGGCTTC TCTGGCTCCT CCAGCCCCCG CAGATGCCCC GGCCTGGCTG
    CACTTCGTGG ACCGACATCG GGAGCAACTG GTGGCTCGGG TGACGTCGGT GGATCCTGTG
    CTGGACATGC TGCATGGGCA GGTGTTGAGC GAGGAGCAGT ACGAGAGGGT GCGGGCCGAG
    GCCACGACCC CCAGCCAGAT GCGGAAGCTG TTCAGTTTCA GCAGGTCGTG GGACCGGGCC
    TGCAAGGACC AGGTGTACCA GGCTCTGAAG GAGGCCCACC CTCACCTCAT CATGGAACTC
    TGGGAGAAGT GGGGCGGCAA CAGTGGCAAG CAGGCGCCTC CTGGCCAGCC CAGCAGCTGC
    ATCTCCAGCA CCTGCCATGA GGCCTGGCTC TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619938.1
    CDS243..4538
    Translation

    Target ORF information:

    RefSeq Version XM_005619881.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGGA
    ATCACAGAAA GGTATCAGCA CCAGAGGAGA AAGAAGCCGT ACCCCACATG GTCGTGGGAA
    GACAAGGACC TGCACCAGAT ATTCATACAG CTGCTGCTTC TGCATCAGCC TTACCCTAGA
    GGCCACGAGT CCTTAACCAA GGGAAGATGG CACCATGGGG CAGAGAAGCA AGGACATTTG
    ATTGAGGTCG AAGACCTATT TGGCCCAGGC CTAGGTGCCC AGGAAGAGCC CCAAACAGTC
    ATACTGCACG GGGTTGCAGG CATCGGGAAG TCAACACTGG CCAGGCAGAT AAGGACGGCG
    TGGAAGGAAG GCCAGCTCTA CAAGGACCGC TTCCGACATG TCTTCTACTT CAACTGCAGA
    GAGCTGGCCC GGTCCGAGAC CATGAGCCTC ACTGAGCTCA TCACCAAGGC CCAGGCCGGC
    CCTGTACTTC CCGCTGAACA GATCCTGTCT CAGCCAGGGC GGCTGCTCTT CATCCTCGAT
    GATTTGGATG AGCCAAGATG GGTCTTAGGG GAGCAGGAGT CAGAGCTGCA TTCGTCCTGG
    AGCAAGCAGC GGCCGGTGCA CGCACTGCTG GGCAGCTTGC TGGGCAAAAC TGTGCTTCCC
    GAGGTGTCCC TGCTGGTTAC AGTGCGGACC ACAGCTCTGC GGAGGCTTGT TCCCTCTGTG
    GGGCAGCTAT GTTGGGTGGA GGTCCTGGGG TTCTCCGAGT CCGGCAGGAG GGAGTACTTC
    TACAAGTATT TCAAGAACAA AAGCCAAGCC ACCCAAGCCT TCAGCTTGGT TGAGTTAAAC
    CCAACGCTGT TAACCATGTG CCTCGTGCCC TTGGTGTCCT GGCTGCTGTG CACCTGCCTG
    AAGAAGCAGA TGCAGCGAGG AGAGGAGCTC TCACTGGGTT CATCCCAGAC CACCACGGCC
    CTCTGGCTAT ACTACCTCTC CCAGGCTCTC CCAGCCCAAC TCCTTGGGGC GCAGCTGAGG
    GGCTTCTGCT CCCTGGCCGC TGAGGGCTTC TGGCAGGGAA CGAACCTGCT CAGCAGGAGG
    GACCTCAGGA AGCACGGGTT CGATGAGGCC ACCATCTCCA CTTTCTTAAA GACAGGCATC
    CTCCGAAAGC ATCCCTCCCC TCTGAACTAC CACCTGGCTC ACCGCTGTCT CCAGGAGTTC
    TTTGCAGCCG TGTCCTGCGT GCTGGGGGGT GAGGATGGCG GAAGCCCTCA CCCCGACAGC
    ATCAGAGGGG CAGAGAAACT GCTAGAGGTG TATGAGGGAC ATGACCTGTT TGGGGCCCCA
    ACCACGCATT TCCTCTTTGG CCTTCTGAGT GAGCAAGGGG AGCGAGAGAT GGAGGCGGTG
    TTCCAGTGCA AGCTGCCCCG GGAGAGGAAG CGGGACCTGC TGCGCTGGGC TGAGGCGGGG
    CTCTGGCCTC GGCTCTCCGC ACCTCAGCCA TGCTCTCTGC ACTTGCTCCA CTGCCTGTAC
    GAGATTCAGG ACGAGGACTT GCTGACACGG GCCATGGCCC ATTTCCATGG AAGGAGCATG
    TGTGTCCAAA CTGGCGTGGA GTTCCTGGTG TGCACTTTCT GCTTTAAATT CTGCTCCCAC
    GTGAAAAGGC TTCAACTGAA TGAGAGCCGG TGGGATGGAC AAGCATGGAG GCCCTCCAGT
    GTGGCTCTGT TCAGCTGGGT CCCAGTCACA GATGCCTGCT GGCAGGCTCT CTTTTCCACT
    CTCCGGGCCT CTGGAAGCCT GAAGGAGCTG GACCTGAGTG GAAATCACCT GAGCCACCCC
    GCAGTTCAGA GTCTTTGTAA AGTCCTGAAA TTCCCTTGCT GCCACTTGGA GACTGTGCGG
    TTGGCCGGCT GCGGCCTGAC GGCGGAAGGC TGCAGCGACC TGGCCTCTGC GCTGAGTGCC
    AGCCCGGCCC TGGCGGAGCT GGAGCTGAGT TTCAACTTGC TCCTGGACGC TGGAGCCCAG
    CATCTGAGCA GGGGGCTGAG GCAGCCGGCC TGCAGGCTCC GCCGGCTGCT GCTGGCTGGC
    TGCAGCCTCA CCTCCCGCTG CTGCGGGGCC CTGGCCTCCG CGCTCAGCAC CAGCCCCCTC
    CTCACGGAAC TAGACCTGCA GCAGAACGAG CTGGGCGACG TCGGCGTGCG GCTGCTGTGC
    GGAGGCCTTG GGCACCCCAC CTGCCAGCTC ACGCTGCTGT GGCTGGACCA GACCCAGTTA
    AGTGAGGAGA TGACACAGAT GCTGAGGGCC CTGCAGGAGG AGAAGCCTCA GCTGTTCGTC
    TGCAGCAAAT GGAAGCCAAG GGCGACCGTC CCCACCGAGG GCCTGAGTCG GGGAGAGACA
    AGTGGCACGT CCTCACTCAA GCAGCAGAGA CAAGGATCAG AGAGAAGCTG CCCTCAGGTA
    GGCCAGGTGC AGCTGGGTCG CCCACCCTCT CCTGCTGCCC CGTGGGACCT GAACACAGAG
    CCTCTGAGGA TCGGGGATGG CTTCTGGGGC CCCCTGGGGC CTGTGGCTCC CGAGTGGCTG
    GCCGAGGATG GAAGCCTCTA CAGGGTTCAC CTGCCTGTGG CCGGCTCCTA CCACTGGCCC
    GACACAGGCC TCCGTTTTGA GGTGAGAGGA CCAGCAACCA TTGACATTGA ATTCTGCGTC
    TGGGACCAGC ACCTGCCCAG GGCCGGCCTA TGGCACAGCT GGATGGTGGC GGGGCCTCTG
    TTTGACATCA AGGCTGAGCC AGGGGCCGTG GCTACTGTGT GCCTCCCTCA CTTCGTGGAC
    CTGCAAGGTA GCAGCGTGGA CACCTCCCTG TTCTACGCGG CCCACTTAAA AGATGAGGGG
    ATGCTGGTGG AGACGCCAGC CAGGGTGGAG GCAGACCACG TAGTCCTGGA GAACCCCAGC
    TTCTCCCCCA TAGGAGTCCT GCTGAGGACC ATCCACGCCG CCCTGCGCTT CCTCCCCGTC
    ACCTCTGTTG TGCTGCTGTA CCATCACCTG AGGCCCGGGG AAGTCGCCTT CCACCTATAC
    CTCATCCCCA GTGACTACTC CATCCGCAAG GCCATAGATA ATGAAGAGAA AAAGTTCCAG
    TTTGTGCAGA TACACAAGCC GCCCCCACTG GCCTCGCTCT ACATGGGCTC CCGCTACACT
    GTGTCTGGCT CAGAGAAGCT AGAGATAATC CCCAAGGAGC TGGAGCTCTG CTACCGCAGC
    CCCAGGGAAC CCCAGCTCTT CTCTGAGTTC TACGTCAGCC ACTTGGGGTC GGGGATCAGG
    CTGCAAGTGA GAGACAAAAA GGATGACACA GTGGTGTGGG AAGCCCTGGT GAAACCAGGG
    GATCTCCGGC CTGTGGCTTC TCTGGCTCCT CCAGCCCCCG CAGATGCCCC GGCCTGGCTG
    CACTTCGTGG ACCGACATCG GGAGCAACTG GTGGCTCGGG TGACGTCGGT GGATCCTGTG
    CTGGACATGC TGCATGGGCA GGTGTTGAGC GAGGAGCAGT ACGAGAGGGT GCGGGCCGAG
    GCCACGACCC CCAGCCAGAT GCGGAAGCTG TTCAGTTTCA GCAGGTCGTG GGACCGGGCC
    TGCAAGGACC AGGTGTACCA GGCTCTGAAG GAGGCCCACC CTCACCTCAT CATGGAACTC
    TGGGAGAAGT GGGGCGGCAA CAGTGGCAAG CAGGCGCCTC CTGGCCAGCC CAGCAGCTGC
    ATCTCCAGCA CCTGCCATGA GGCCTGGCTC TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06117
    Clone ID Related Accession (Same CDS sequence) XM_005619881.2 , XM_005619881.3
    Accession Version XM_005619881.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619881.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGGA
    ATCACAGAAA GGTATCAGCA CCAGAGGAGA AAGAAGCCGT ACCCCACATG GTCGTGGGAA
    GACAAGGACC TGCACCAGAT ATTCATACAG CTGCTGCTTC TGCATCAGCC TTACCCTAGA
    GGCCACGAGT CCTTAACCAA GGGAAGATGG CACCATGGGG CAGAGAAGCA AGGACATTTG
    ATTGAGGTCG AAGACCTATT TGGCCCAGGC CTAGGTGCCC AGGAAGAGCC CCAAACAGTC
    ATACTGCACG GGGTTGCAGG CATCGGGAAG TCAACACTGG CCAGGCAGAT AAGGACGGCG
    TGGAAGGAAG GCCAGCTCTA CAAGGACCGC TTCCGACATG TCTTCTACTT CAACTGCAGA
    GAGCTGGCCC GGTCCGAGAC CATGAGCCTC ACTGAGCTCA TCACCAAGGC CCAGGCCGGC
    CCTGTACTTC CCGCTGAACA GATCCTGTCT CAGCCAGGGC GGCTGCTCTT CATCCTCGAT
    GATTTGGATG AGCCAAGATG GGTCTTAGGG GAGCAGGAGT CAGAGCTGCA TTCGTCCTGG
    AGCAAGCAGC GGCCGGTGCA CGCACTGCTG GGCAGCTTGC TGGGCAAAAC TGTGCTTCCC
    GAGGTGTCCC TGCTGGTTAC AGTGCGGACC ACAGCTCTGC GGAGGCTTGT TCCCTCTGTG
    GGGCAGCTAT GTTGGGTGGA GGTCCTGGGG TTCTCCGAGT CCGGCAGGAG GGAGTACTTC
    TACAAGTATT TCAAGAACAA AAGCCAAGCC ACCCAAGCCT TCAGCTTGGT TGAGTTAAAC
    CCAACGCTGT TAACCATGTG CCTCGTGCCC TTGGTGTCCT GGCTGCTGTG CACCTGCCTG
    AAGAAGCAGA TGCAGCGAGG AGAGGAGCTC TCACTGGGTT CATCCCAGAC CACCACGGCC
    CTCTGGCTAT ACTACCTCTC CCAGGCTCTC CCAGCCCAAC TCCTTGGGGC GCAGCTGAGG
    GGCTTCTGCT CCCTGGCCGC TGAGGGCTTC TGGCAGGGAA CGAACCTGCT CAGCAGGAGG
    GACCTCAGGA AGCACGGGTT CGATGAGGCC ACCATCTCCA CTTTCTTAAA GACAGGCATC
    CTCCGAAAGC ATCCCTCCCC TCTGAACTAC CACCTGGCTC ACCGCTGTCT CCAGGAGTTC
    TTTGCAGCCG TGTCCTGCGT GCTGGGGGGT GAGGATGGCG GAAGCCCTCA CCCCGACAGC
    ATCAGAGGGG CAGAGAAACT GCTAGAGGTG TATGAGGGAC ATGACCTGTT TGGGGCCCCA
    ACCACGCATT TCCTCTTTGG CCTTCTGAGT GAGCAAGGGG AGCGAGAGAT GGAGGCGGTG
    TTCCAGTGCA AGCTGCCCCG GGAGAGGAAG CGGGACCTGC TGCGCTGGGC TGAGGCGGGG
    CTCTGGCCTC GGCTCTCCGC ACCTCAGCCA TGCTCTCTGC ACTTGCTCCA CTGCCTGTAC
    GAGATTCAGG ACGAGGACTT GCTGACACGG GCCATGGCCC ATTTCCATGG AAGGAGCATG
    TGTGTCCAAA CTGGCGTGGA GTTCCTGGTG TGCACTTTCT GCTTTAAATT CTGCTCCCAC
    GTGAAAAGGC TTCAACTGAA TGAGAGCCGG TGGGATGGAC AAGCATGGAG GCCCTCCAGT
    GTGGCTCTGT TCAGCTGGGT CCCAGTCACA GATGCCTGCT GGCAGGCTCT CTTTTCCACT
    CTCCGGGCCT CTGGAAGCCT GAAGGAGCTG GACCTGAGTG GAAATCACCT GAGCCACCCC
    GCAGTTCAGA GTCTTTGTAA AGTCCTGAAA TTCCCTTGCT GCCACTTGGA GACTGTGCGG
    TTGGCCGGCT GCGGCCTGAC GGCGGAAGGC TGCAGCGACC TGGCCTCTGC GCTGAGTGCC
    AGCCCGGCCC TGGCGGAGCT GGAGCTGAGT TTCAACTTGC TCCTGGACGC TGGAGCCCAG
    CATCTGAGCA GGGGGCTGAG GCAGCCGGCC TGCAGGCTCC GCCGGCTGCT GCTGGCTGGC
    TGCAGCCTCA CCTCCCGCTG CTGCGGGGCC CTGGCCTCCG CGCTCAGCAC CAGCCCCCTC
    CTCACGGAAC TAGACCTGCA GCAGAACGAG CTGGGCGACG TCGGCGTGCG GCTGCTGTGC
    GGAGGCCTTG GGCACCCCAC CTGCCAGCTC ACGCTGCTGT GGCTGGACCA GACCCAGTTA
    AGTGAGGAGA TGACACAGAT GCTGAGGGCC CTGCAGGAGG AGAAGCCTCA GCTGTTCGTC
    TGCAGCAAAT GGAAGCCAAG GGCGACCGTC CCCACCGAGG GCCTGAGTCG GGGAGAGACA
    AGTGGCACGT CCTCACTCAA GCAGCAGAGA CAAGGATCAG AGAGAAGCTG CCCTCAGGTA
    GGCCAGGTGC AGCTGGGTCG CCCACCCTCT CCTGCTGCCC CGTGGGACCT GAACACAGAG
    CCTCTGAGGA TCGGGGATGG CTTCTGGGGC CCCCTGGGGC CTGTGGCTCC CGAGTGGCTG
    GCCGAGGATG GAAGCCTCTA CAGGGTTCAC CTGCCTGTGG CCGGCTCCTA CCACTGGCCC
    GACACAGGCC TCCGTTTTGA GGTGAGAGGA CCAGCAACCA TTGACATTGA ATTCTGCGTC
    TGGGACCAGC ACCTGCCCAG GGCCGGCCTA TGGCACAGCT GGATGGTGGC GGGGCCTCTG
    TTTGACATCA AGGCTGAGCC AGGGGCCGTG GCTACTGTGT GCCTCCCTCA CTTCGTGGAC
    CTGCAAGGTA GCAGCGTGGA CACCTCCCTG TTCTACGCGG CCCACTTAAA AGATGAGGGG
    ATGCTGGTGG AGACGCCAGC CAGGGTGGAG GCAGACCACG TAGTCCTGGA GAACCCCAGC
    TTCTCCCCCA TAGGAGTCCT GCTGAGGACC ATCCACGCCG CCCTGCGCTT CCTCCCCGTC
    ACCTCTGTTG TGCTGCTGTA CCATCACCTG AGGCCCGGGG AAGTCGCCTT CCACCTATAC
    CTCATCCCCA GTGACTACTC CATCCGCAAG GCCATAGATA ATGAAGAGAA AAAGTTCCAG
    TTTGTGCAGA TACACAAGCC GCCCCCACTG GCCTCGCTCT ACATGGGCTC CCGCTACACT
    GTGTCTGGCT CAGAGAAGCT AGAGATAATC CCCAAGGAGC TGGAGCTCTG CTACCGCAGC
    CCCAGGGAAC CCCAGCTCTT CTCTGAGTTC TACGTCAGCC ACTTGGGGTC GGGGATCAGG
    CTGCAAGTGA GAGACAAAAA GGATGACACA GTGGTGTGGG AAGCCCTGGT GAAACCAGGG
    GATCTCCGGC CTGTGGCTTC TCTGGCTCCT CCAGCCCCCG CAGATGCCCC GGCCTGGCTG
    CACTTCGTGG ACCGACATCG GGAGCAACTG GTGGCTCGGG TGACGTCGGT GGATCCTGTG
    CTGGACATGC TGCATGGGCA GGTGTTGAGC GAGGAGCAGT ACGAGAGGGT GCGGGCCGAG
    GCCACGACCC CCAGCCAGAT GCGGAAGCTG TTCAGTTTCA GCAGGTCGTG GGACCGGGCC
    TGCAAGGACC AGGTGTACCA GGCTCTGAAG GAGGCCCACC CTCACCTCAT CATGGAACTC
    TGGGAGAAGT GGGGCGGCAA CAGTGGCAAG CAGGCGCCTC CTGGCCAGCC CAGCAGCTGC
    ATCTCCAGCA CCTGCCATGA GGCCTGGCTC TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619938.1
    CDS1807..6102
    Translation

    Target ORF information:

    RefSeq Version XM_005619881.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NLR family pyrin domain containing 1 (NLRP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619881.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAA GACCCTTCCT CGTGCAAGTA CCAAGAAGGC
    CTTCCAGACT CCCCAGAGTT TCAAGGTTCA CCACACCAGG AGTCACCCAG TGCCCCCATG
    CCCACGGCAG TGCTAGGGGG CTGGGAGGCC CCACCTCAGC CCAGCCCAGT GCCCGGGAAG
    CAGGAGGCGC CCAAGGCTGG GTGGCCCGTG GCTGGAACAT CTGGAAACCA CAACCCAGGA
    ATCACAGAAA GGTATCAGCA CCAGAGGAGA AAGAAGCCGT ACCCCACATG GTCGTGGGAA
    GACAAGGACC TGCACCAGAT ATTCATACAG CTGCTGCTTC TGCATCAGCC TTACCCTAGA
    GGCCACGAGT CCTTAACCAA GGGAAGATGG CACCATGGGG CAGAGAAGCA AGGACATTTG
    ATTGAGGTCG AAGACCTATT TGGCCCAGGC CTAGGTGCCC AGGAAGAGCC CCAAACAGTC
    ATACTGCACG GGGTTGCAGG CATCGGGAAG TCAACACTGG CCAGGCAGAT AAGGACGGCG
    TGGAAGGAAG GCCAGCTCTA CAAGGACCGC TTCCGACATG TCTTCTACTT CAACTGCAGA
    GAGCTGGCCC GGTCCGAGAC CATGAGCCTC ACTGAGCTCA TCACCAAGGC CCAGGCCGGC
    CCTGTACTTC CCGCTGAACA GATCCTGTCT CAGCCAGGGC GGCTGCTCTT CATCCTCGAT
    GATTTGGATG AGCCAAGATG GGTCTTAGGG GAGCAGGAGT CAGAGCTGCA TTCGTCCTGG
    AGCAAGCAGC GGCCGGTGCA CGCACTGCTG GGCAGCTTGC TGGGCAAAAC TGTGCTTCCC
    GAGGTGTCCC TGCTGGTTAC AGTGCGGACC ACAGCTCTGC GGAGGCTTGT TCCCTCTGTG
    GGGCAGCTAT GTTGGGTGGA GGTCCTGGGG TTCTCCGAGT CCGGCAGGAG GGAGTACTTC
    TACAAGTATT TCAAGAACAA AAGCCAAGCC ACCCAAGCCT TCAGCTTGGT TGAGTTAAAC
    CCAACGCTGT TAACCATGTG CCTCGTGCCC TTGGTGTCCT GGCTGCTGTG CACCTGCCTG
    AAGAAGCAGA TGCAGCGAGG AGAGGAGCTC TCACTGGGTT CATCCCAGAC CACCACGGCC
    CTCTGGCTAT ACTACCTCTC CCAGGCTCTC CCAGCCCAAC TCCTTGGGGC GCAGCTGAGG
    GGCTTCTGCT CCCTGGCCGC TGAGGGCTTC TGGCAGGGAA CGAACCTGCT CAGCAGGAGG
    GACCTCAGGA AGCACGGGTT CGATGAGGCC ACCATCTCCA CTTTCTTAAA GACAGGCATC
    CTCCGAAAGC ATCCCTCCCC TCTGAACTAC CACCTGGCTC ACCGCTGTCT CCAGGAGTTC
    TTTGCAGCCG TGTCCTGCGT GCTGGGGGGT GAGGATGGCG GAAGCCCTCA CCCCGACAGC
    ATCAGAGGGG CAGAGAAACT GCTAGAGGTG TATGAGGGAC ATGACCTGTT TGGGGCCCCA
    ACCACGCATT TCCTCTTTGG CCTTCTGAGT GAGCAAGGGG AGCGAGAGAT GGAGGCGGTG
    TTCCAGTGCA AGCTGCCCCG GGAGAGGAAG CGGGACCTGC TGCGCTGGGC TGAGGCGGGG
    CTCTGGCCTC GGCTCTCCGC ACCTCAGCCA TGCTCTCTGC ACTTGCTCCA CTGCCTGTAC
    GAGATTCAGG ACGAGGACTT GCTGACACGG GCCATGGCCC ATTTCCATGG AAGGAGCATG
    TGTGTCCAAA CTGGCGTGGA GTTCCTGGTG TGCACTTTCT GCTTTAAATT CTGCTCCCAC
    GTGAAAAGGC TTCAACTGAA TGAGAGCCGG TGGGATGGAC AAGCATGGAG GCCCTCCAGT
    GTGGCTCTGT TCAGCTGGGT CCCAGTCACA GATGCCTGCT GGCAGGCTCT CTTTTCCACT
    CTCCGGGCCT CTGGAAGCCT GAAGGAGCTG GACCTGAGTG GAAATCACCT GAGCCACCCC
    GCAGTTCAGA GTCTTTGTAA AGTCCTGAAA TTCCCTTGCT GCCACTTGGA GACTGTGCGG
    TTGGCCGGCT GCGGCCTGAC GGCGGAAGGC TGCAGCGACC TGGCCTCTGC GCTGAGTGCC
    AGCCCGGCCC TGGCGGAGCT GGAGCTGAGT TTCAACTTGC TCCTGGACGC TGGAGCCCAG
    CATCTGAGCA GGGGGCTGAG GCAGCCGGCC TGCAGGCTCC GCCGGCTGCT GCTGGCTGGC
    TGCAGCCTCA CCTCCCGCTG CTGCGGGGCC CTGGCCTCCG CGCTCAGCAC CAGCCCCCTC
    CTCACGGAAC TAGACCTGCA GCAGAACGAG CTGGGCGACG TCGGCGTGCG GCTGCTGTGC
    GGAGGCCTTG GGCACCCCAC CTGCCAGCTC ACGCTGCTGT GGCTGGACCA GACCCAGTTA
    AGTGAGGAGA TGACACAGAT GCTGAGGGCC CTGCAGGAGG AGAAGCCTCA GCTGTTCGTC
    TGCAGCAAAT GGAAGCCAAG GGCGACCGTC CCCACCGAGG GCCTGAGTCG GGGAGAGACA
    AGTGGCACGT CCTCACTCAA GCAGCAGAGA CAAGGATCAG AGAGAAGCTG CCCTCAGGTA
    GGCCAGGTGC AGCTGGGTCG CCCACCCTCT CCTGCTGCCC CGTGGGACCT GAACACAGAG
    CCTCTGAGGA TCGGGGATGG CTTCTGGGGC CCCCTGGGGC CTGTGGCTCC CGAGTGGCTG
    GCCGAGGATG GAAGCCTCTA CAGGGTTCAC CTGCCTGTGG CCGGCTCCTA CCACTGGCCC
    GACACAGGCC TCCGTTTTGA GGTGAGAGGA CCAGCAACCA TTGACATTGA ATTCTGCGTC
    TGGGACCAGC ACCTGCCCAG GGCCGGCCTA TGGCACAGCT GGATGGTGGC GGGGCCTCTG
    TTTGACATCA AGGCTGAGCC AGGGGCCGTG GCTACTGTGT GCCTCCCTCA CTTCGTGGAC
    CTGCAAGGTA GCAGCGTGGA CACCTCCCTG TTCTACGCGG CCCACTTAAA AGATGAGGGG
    ATGCTGGTGG AGACGCCAGC CAGGGTGGAG GCAGACCACG TAGTCCTGGA GAACCCCAGC
    TTCTCCCCCA TAGGAGTCCT GCTGAGGACC ATCCACGCCG CCCTGCGCTT CCTCCCCGTC
    ACCTCTGTTG TGCTGCTGTA CCATCACCTG AGGCCCGGGG AAGTCGCCTT CCACCTATAC
    CTCATCCCCA GTGACTACTC CATCCGCAAG GCCATAGATA ATGAAGAGAA AAAGTTCCAG
    TTTGTGCAGA TACACAAGCC GCCCCCACTG GCCTCGCTCT ACATGGGCTC CCGCTACACT
    GTGTCTGGCT CAGAGAAGCT AGAGATAATC CCCAAGGAGC TGGAGCTCTG CTACCGCAGC
    CCCAGGGAAC CCCAGCTCTT CTCTGAGTTC TACGTCAGCC ACTTGGGGTC GGGGATCAGG
    CTGCAAGTGA GAGACAAAAA GGATGACACA GTGGTGTGGG AAGCCCTGGT GAAACCAGGG
    GATCTCCGGC CTGTGGCTTC TCTGGCTCCT CCAGCCCCCG CAGATGCCCC GGCCTGGCTG
    CACTTCGTGG ACCGACATCG GGAGCAACTG GTGGCTCGGG TGACGTCGGT GGATCCTGTG
    CTGGACATGC TGCATGGGCA GGTGTTGAGC GAGGAGCAGT ACGAGAGGGT GCGGGCCGAG
    GCCACGACCC CCAGCCAGAT GCGGAAGCTG TTCAGTTTCA GCAGGTCGTG GGACCGGGCC
    TGCAAGGACC AGGTGTACCA GGCTCTGAAG GAGGCCCACC CTCACCTCAT CATGGAACTC
    TGGGAGAAGT GGGGCGGCAA CAGTGGCAAG CAGGCGCCTC CTGGCCAGCC CAGCAGCTGC
    ATCTCCAGCA CCTGCCATGA GGCCTGGCTC TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06118
    Clone ID Related Accession (Same CDS sequence) XM_005619882.2
    Accession Version XM_005619882.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NACHT, LRR and PYD domains-containing protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619882.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAC CCTTCCTCGT GCAAGTACCA AGAAGGCCTT
    CCAGACTCCC CAGAGTTTCA AGGTTCACCA CACCAGGAGT CACCCAGTGC CCCCATGCCC
    ACGGCAGTGC TAGGGGGCTG GGAGGCCCCA CCTCAGCCCA GCCCAGTGCC CGGGAAGCAG
    GAGGCGCCCA AGGCTGGGTG GCCCGTGGCT GGAACATCTG GAAACCACAA CCCAGCAGGA
    ATCACAGAAA GGTATCAGCA CCAGAGGAGA AAGAAGCCGT ACCCCACATG GTCGTGGGAA
    GACAAGGACC TGCACCAGAT ATTCATACAG CTGCTGCTTC TGCATCAGCC TTACCCTAGA
    GGCCACGAGT CCTTAACCAA GGGAAGATGG CACCATGGGG CAGAGAAGCA AGGACATTTG
    ATTGAGGTCG AAGACCTATT TGGCCCAGGC CTAGGTGCCC AGGAAGAGCC CCAAACAGTC
    ATACTGCACG GGGTTGCAGG CATCGGGAAG TCAACACTGG CCAGGCAGAT AAGGACGGCG
    TGGAAGGAAG GCCAGCTCTA CAAGGACCGC TTCCGACATG TCTTCTACTT CAACTGCAGA
    GAGCTGGCCC GGTCCGAGAC CATGAGCCTC ACTGAGCTCA TCACCAAGGC CCAGGCCGGC
    CCTGTACTTC CCGCTGAACA GATCCTGTCT CAGCCAGGGC GGCTGCTCTT CATCCTCGAT
    GATTTGGATG AGCCAAGATG GGTCTTAGGG GAGCAGGAGT CAGAGCTGCA TTCGTCCTGG
    AGCAAGCAGC GGCCGGTGCA CGCACTGCTG GGCAGCTTGC TGGGCAAAAC TGTGCTTCCC
    GAGGTGTCCC TGCTGGTTAC AGTGCGGACC ACAGCTCTGC GGAGGCTTGT TCCCTCTGTG
    GGGCAGCTAT GTTGGGTGGA GGTCCTGGGG TTCTCCGAGT CCGGCAGGAG GGAGTACTTC
    TACAAGTATT TCAAGAACAA AAGCCAAGCC ACCCAAGCCT TCAGCTTGGT TGAGTTAAAC
    CCAACGCTGT TAACCATGTG CCTCGTGCCC TTGGTGTCCT GGCTGCTGTG CACCTGCCTG
    AAGAAGCAGA TGCAGCGAGG AGAGGAGCTC TCACTGGGTT CATCCCAGAC CACCACGGCC
    CTCTGGCTAT ACTACCTCTC CCAGGCTCTC CCAGCCCAAC TCCTTGGGGC GCAGCTGAGG
    GGCTTCTGCT CCCTGGCCGC TGAGGGCTTC TGGCAGGGAA CGAACCTGCT CAGCAGGAGG
    GACCTCAGGA AGCACGGGTT CGATGAGGCC ACCATCTCCA CTTTCTTAAA GACAGGCATC
    CTCCGAAAGC ATCCCTCCCC TCTGAACTAC CACCTGGCTC ACCGCTGTCT CCAGGAGTTC
    TTTGCAGCCG TGTCCTGCGT GCTGGGGGGT GAGGATGGCG GAAGCCCTCA CCCCGACAGC
    ATCAGAGGGG CAGAGAAACT GCTAGAGGTG TATGAGGGAC ATGACCTGTT TGGGGCCCCA
    ACCACGCATT TCCTCTTTGG CCTTCTGAGT GAGCAAGGGG AGCGAGAGAT GGAGGCGGTG
    TTCCAGTGCA AGCTGCCCCG GGAGAGGAAG CGGGACCTGC TGCGCTGGGC TGAGGCGGGG
    CTCTGGCCTC GGCTCTCCGC ACCTCAGCCA TGCTCTCTGC ACTTGCTCCA CTGCCTGTAC
    GAGATTCAGG ACGAGGACTT GCTGACACGG GCCATGGCCC ATTTCCATGG AAGGAGCATG
    TGTGTCCAAA CTGGCGTGGA GTTCCTGGTG TGCACTTTCT GCTTTAAATT CTGCTCCCAC
    GTGAAAAGGC TTCAACTGAA TGAGAGCCGG TGGGATGGAC AAGCATGGAG GCCCTCCAGT
    GTGGCTCTGT TCAGCTGGGT CCCAGTCACA GATGCCTGCT GGCAGGCTCT CTTTTCCACT
    CTCCGGGCCT CTGGAAGCCT GAAGGAGCTG GACCTGAGTG GAAATCACCT GAGCCACCCC
    GCAGTTCAGA GTCTTTGTAA AGTCCTGAAA TTCCCTTGCT GCCACTTGGA GACTGTGCGG
    TTGGCCGGCT GCGGCCTGAC GGCGGAAGGC TGCAGCGACC TGGCCTCTGC GCTGAGTGCC
    AGCCCGGCCC TGGCGGAGCT GGAGCTGAGT TTCAACTTGC TCCTGGACGC TGGAGCCCAG
    CATCTGAGCA GGGGGCTGAG GCAGCCGGCC TGCAGGCTCC GCCGGCTGCT GCTGGCTGGC
    TGCAGCCTCA CCTCCCGCTG CTGCGGGGCC CTGGCCTCCG CGCTCAGCAC CAGCCCCCTC
    CTCACGGAAC TAGACCTGCA GCAGAACGAG CTGGGCGACG TCGGCGTGCG GCTGCTGTGC
    GGAGGCCTTG GGCACCCCAC CTGCCAGCTC ACGCTGCTGT GGCTGGACCA GACCCAGTTA
    AGTGAGGAGA TGACACAGAT GCTGAGGGCC CTGCAGGAGG AGAAGCCTCA GCTGTTCGTC
    TGCAGCAAAT GGAAGCCAAG GGCGACCGTC CCCACCGAGG GCCTGAGTCG GGGAGAGACA
    AGTGGCACGT CCTCACTCAA GCAGCAGAGA CAAGGATCAG AGAGAAGCTG CCCTCAGGTA
    GGCCAGGTGC AGCTGGGTCG CCCACCCTCT CCTGCTGCCC CGTGGGACCT GAACACAGAG
    CCTCTGAGGA TCGGGGATGG CTTCTGGGGC CCCCTGGGGC CTGTGGCTCC CGAGTGGCTG
    GCCGAGGATG GAAGCCTCTA CAGGGTTCAC CTGCCTGTGG CCGGCTCCTA CCACTGGCCC
    GACACAGGCC TCCGTTTTGA GGTGAGAGGA CCAGCAACCA TTGACATTGA ATTCTGCGTC
    TGGGACCAGC ACCTGCCCAG GGCCGGCCTA TGGCACAGCT GGATGGTGGC GGGGCCTCTG
    TTTGACATCA AGGCTGAGCC AGGGGCCGTG GCTACTGTGT GCCTCCCTCA CTTCGTGGAC
    CTGCAAGGTA GCAGCGTGGA CACCTCCCTG TTCTACGCGG CCCACTTAAA AGATGAGGGG
    ATGCTGGTGG AGACGCCAGC CAGGGTGGAG GCAGACCACG TAGTCCTGGA GAACCCCAGC
    TTCTCCCCCA TAGGAGTCCT GCTGAGGACC ATCCACGCCG CCCTGCGCTT CCTCCCCGTC
    ACCTCTGTTG TGCTGCTGTA CCATCACCTG AGGCCCGGGG AAGTCGCCTT CCACCTATAC
    CTCATCCCCA GTGACTACTC CATCCGCAAG GCCATAGATA ATGAAGAGAA AAAGTTCCAG
    TTTGTGCAGA TACACAAGCC GCCCCCACTG GCCTCGCTCT ACATGGGCTC CCGCTACACT
    GTGTCTGGCT CAGAGAAGCT AGAGATAATC CCCAAGGAGC TGGAGCTCTG CTACCGCAGC
    CCCAGGGAAC CCCAGCTCTT CTCTGAGTTC TACGTCAGCC ACTTGGGGTC GGGGATCAGG
    CTGCAAGTGA GAGACAAAAA GGATGACACA GTGGTGTGGG AAGCCCTGGT GAAACCAGGG
    GATCTCCGGC CTGTGGCTTC TCTGGCTCCT CCAGCCCCCG CAGATGCCCC GGCCTGGCTG
    CACTTCGTGG ACCGACATCG GGAGCAACTG GTGGCTCGGG TGACGTCGGT GGATCCTGTG
    CTGGACATGC TGCATGGGCA GGTGTTGAGC GAGGAGCAGT ACGAGAGGGT GCGGGCCGAG
    GCCACGACCC CCAGCCAGAT GCGGAAGCTG TTCAGTTTCA GCAGGTCGTG GGACCGGGCC
    TGCAAGGACC AGGTGTACCA GGCTCTGAAG GAGGCCCACC CTCACCTCAT CATGGAACTC
    TGGGAGAAGT GGGGCGGCAA CAGTGGCAAG CAGGCGCCTC CTGGCCAGCC CAGCAGCTGC
    ATCTCCAGCA CCTGCCATGA GGCCTGGCTC TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619939.1
    CDS243..4538
    Translation

    Target ORF information:

    RefSeq Version XM_005619882.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NLR family, pyrin domain containing 1 (NLRP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCCTGCA GAGTCCAGTG GCAGCTGGCC TGGTACCTGG AGATGATGGG GAAAGAGGAG 
    CTAAAGGAGT TCCAGCTTCG GCTGCTGGAA CAGCAGTTCT GGGGGGACCC TCCCCACGCC
    CTGCGGGCCC AGCTGGGGAA GGCACGCGGC TTGGAGGTGG CCTCGCGCCT GGTGGCCCAG
    TATGGGGAGC AGCAGGCCTG GGTGCTGGCC CTCCGCACTT GGGAGGAAAT GGGCCTGAGC
    CGGCTCTGCG CCCAGTCCCG GGCAGAGGCA GGCTTGATGT CAGCCGCACA CCCCCTATCC
    CCCGGCTCCC CGATGGCACC CAGCATGCAG TCCCCAAACT CACCTATCTC CACTGAGGTG
    CTGAGGTTCA TCCAGGACCC AAGGACAAGG AATTCGGGAC AGCCACTGCC ACCACTGCTG
    CCGAACACAT CTGGACACAT GTGGAATGAC CCTTCCTCGT GCAAGTACCA AGAAGGCCTT
    CCAGACTCCC CAGAGTTTCA AGGTTCACCA CACCAGGAGT CACCCAGTGC CCCCATGCCC
    ACGGCAGTGC TAGGGGGCTG GGAGGCCCCA CCTCAGCCCA GCCCAGTGCC CGGGAAGCAG
    GAGGCGCCCA AGGCTGGGTG GCCCGTGGCT GGAACATCTG GAAACCACAA CCCAGCAGGA
    ATCACAGAAA GGTATCAGCA CCAGAGGAGA AAGAAGCCGT ACCCCACATG GTCGTGGGAA
    GACAAGGACC TGCACCAGAT ATTCATACAG CTGCTGCTTC TGCATCAGCC TTACCCTAGA
    GGCCACGAGT CCTTAACCAA GGGAAGATGG CACCATGGGG CAGAGAAGCA AGGACATTTG
    ATTGAGGTCG AAGACCTATT TGGCCCAGGC CTAGGTGCCC AGGAAGAGCC CCAAACAGTC
    ATACTGCACG GGGTTGCAGG CATCGGGAAG TCAACACTGG CCAGGCAGAT AAGGACGGCG
    TGGAAGGAAG GCCAGCTCTA CAAGGACCGC TTCCGACATG TCTTCTACTT CAACTGCAGA
    GAGCTGGCCC GGTCCGAGAC CATGAGCCTC ACTGAGCTCA TCACCAAGGC CCAGGCCGGC
    CCTGTACTTC CCGCTGAACA GATCCTGTCT CAGCCAGGGC GGCTGCTCTT CATCCTCGAT
    GATTTGGATG AGCCAAGATG GGTCTTAGGG GAGCAGGAGT CAGAGCTGCA TTCGTCCTGG
    AGCAAGCAGC GGCCGGTGCA CGCACTGCTG GGCAGCTTGC TGGGCAAAAC TGTGCTTCCC
    GAGGTGTCCC TGCTGGTTAC AGTGCGGACC ACAGCTCTGC GGAGGCTTGT TCCCTCTGTG
    GGGCAGCTAT GTTGGGTGGA GGTCCTGGGG TTCTCCGAGT CCGGCAGGAG GGAGTACTTC
    TACAAGTATT TCAAGAACAA AAGCCAAGCC ACCCAAGCCT TCAGCTTGGT TGAGTTAAAC
    CCAACGCTGT TAACCATGTG CCTCGTGCCC TTGGTGTCCT GGCTGCTGTG CACCTGCCTG
    AAGAAGCAGA TGCAGCGAGG AGAGGAGCTC TCACTGGGTT CATCCCAGAC CACCACGGCC
    CTCTGGCTAT ACTACCTCTC CCAGGCTCTC CCAGCCCAAC TCCTTGGGGC GCAGCTGAGG
    GGCTTCTGCT CCCTGGCCGC TGAGGGCTTC TGGCAGGGAA CGAACCTGCT CAGCAGGAGG
    GACCTCAGGA AGCACGGGTT CGATGAGGCC ACCATCTCCA CTTTCTTAAA GACAGGCATC
    CTCCGAAAGC ATCCCTCCCC TCTGAACTAC CACCTGGCTC ACCGCTGTCT CCAGGAGTTC
    TTTGCAGCCG TGTCCTGCGT GCTGGGGGGT GAGGATGGCG GAAGCCCTCA CCCCGACAGC
    ATCAGAGGGG CAGAGAAACT GCTAGAGGTG TATGAGGGAC ATGACCTGTT TGGGGCCCCA
    ACCACGCATT TCCTCTTTGG CCTTCTGAGT GAGCAAGGGG AGCGAGAGAT GGAGGCGGTG
    TTCCAGTGCA AGCTGCCCCG GGAGAGGAAG CGGGACCTGC TGCGCTGGGC TGAGGCGGGG
    CTCTGGCCTC GGCTCTCCGC ACCTCAGCCA TGCTCTCTGC ACTTGCTCCA CTGCCTGTAC
    GAGATTCAGG ACGAGGACTT GCTGACACGG GCCATGGCCC ATTTCCATGG AAGGAGCATG
    TGTGTCCAAA CTGGCGTGGA GTTCCTGGTG TGCACTTTCT GCTTTAAATT CTGCTCCCAC
    GTGAAAAGGC TTCAACTGAA TGAGAGCCGG TGGGATGGAC AAGCATGGAG GCCCTCCAGT
    GTGGCTCTGT TCAGCTGGGT CCCAGTCACA GATGCCTGCT GGCAGGCTCT CTTTTCCACT
    CTCCGGGCCT CTGGAAGCCT GAAGGAGCTG GACCTGAGTG GAAATCACCT GAGCCACCCC
    GCAGTTCAGA GTCTTTGTAA AGTCCTGAAA TTCCCTTGCT GCCACTTGGA GACTGTGCGG
    TTGGCCGGCT GCGGCCTGAC GGCGGAAGGC TGCAGCGACC TGGCCTCTGC GCTGAGTGCC
    AGCCCGGCCC TGGCGGAGCT GGAGCTGAGT TTCAACTTGC TCCTGGACGC TGGAGCCCAG
    CATCTGAGCA GGGGGCTGAG GCAGCCGGCC TGCAGGCTCC GCCGGCTGCT GCTGGCTGGC
    TGCAGCCTCA CCTCCCGCTG CTGCGGGGCC CTGGCCTCCG CGCTCAGCAC CAGCCCCCTC
    CTCACGGAAC TAGACCTGCA GCAGAACGAG CTGGGCGACG TCGGCGTGCG GCTGCTGTGC
    GGAGGCCTTG GGCACCCCAC CTGCCAGCTC ACGCTGCTGT GGCTGGACCA GACCCAGTTA
    AGTGAGGAGA TGACACAGAT GCTGAGGGCC CTGCAGGAGG AGAAGCCTCA GCTGTTCGTC
    TGCAGCAAAT GGAAGCCAAG GGCGACCGTC CCCACCGAGG GCCTGAGTCG GGGAGAGACA
    AGTGGCACGT CCTCACTCAA GCAGCAGAGA CAAGGATCAG AGAGAAGCTG CCCTCAGGTA
    GGCCAGGTGC AGCTGGGTCG CCCACCCTCT CCTGCTGCCC CGTGGGACCT GAACACAGAG
    CCTCTGAGGA TCGGGGATGG CTTCTGGGGC CCCCTGGGGC CTGTGGCTCC CGAGTGGCTG
    GCCGAGGATG GAAGCCTCTA CAGGGTTCAC CTGCCTGTGG CCGGCTCCTA CCACTGGCCC
    GACACAGGCC TCCGTTTTGA GGTGAGAGGA CCAGCAACCA TTGACATTGA ATTCTGCGTC
    TGGGACCAGC ACCTGCCCAG GGCCGGCCTA TGGCACAGCT GGATGGTGGC GGGGCCTCTG
    TTTGACATCA AGGCTGAGCC AGGGGCCGTG GCTACTGTGT GCCTCCCTCA CTTCGTGGAC
    CTGCAAGGTA GCAGCGTGGA CACCTCCCTG TTCTACGCGG CCCACTTAAA AGATGAGGGG
    ATGCTGGTGG AGACGCCAGC CAGGGTGGAG GCAGACCACG TAGTCCTGGA GAACCCCAGC
    TTCTCCCCCA TAGGAGTCCT GCTGAGGACC ATCCACGCCG CCCTGCGCTT CCTCCCCGTC
    ACCTCTGTTG TGCTGCTGTA CCATCACCTG AGGCCCGGGG AAGTCGCCTT CCACCTATAC
    CTCATCCCCA GTGACTACTC CATCCGCAAG GCCATAGATA ATGAAGAGAA AAAGTTCCAG
    TTTGTGCAGA TACACAAGCC GCCCCCACTG GCCTCGCTCT ACATGGGCTC CCGCTACACT
    GTGTCTGGCT CAGAGAAGCT AGAGATAATC CCCAAGGAGC TGGAGCTCTG CTACCGCAGC
    CCCAGGGAAC CCCAGCTCTT CTCTGAGTTC TACGTCAGCC ACTTGGGGTC GGGGATCAGG
    CTGCAAGTGA GAGACAAAAA GGATGACACA GTGGTGTGGG AAGCCCTGGT GAAACCAGGG
    GATCTCCGGC CTGTGGCTTC TCTGGCTCCT CCAGCCCCCG CAGATGCCCC GGCCTGGCTG
    CACTTCGTGG ACCGACATCG GGAGCAACTG GTGGCTCGGG TGACGTCGGT GGATCCTGTG
    CTGGACATGC TGCATGGGCA GGTGTTGAGC GAGGAGCAGT ACGAGAGGGT GCGGGCCGAG
    GCCACGACCC CCAGCCAGAT GCGGAAGCTG TTCAGTTTCA GCAGGTCGTG GGACCGGGCC
    TGCAAGGACC AGGTGTACCA GGCTCTGAAG GAGGCCCACC CTCACCTCAT CATGGAACTC
    TGGGAGAAGT GGGGCGGCAA CAGTGGCAAG CAGGCGCCTC CTGGCCAGCC CAGCAGCTGC
    ATCTCCAGCA CCTGCCATGA GGCCTGGCTC TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.