ZZEF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following ZZEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZZEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe288037 XM_022418397.1
Latest version!
Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1371.30
$1959.00
OCe06092 XM_546554.6
Latest version!
Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe06093 XM_005619866.3
Latest version!
Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe288036 XM_022418395.1
Latest version!
Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OCe06092 XM_546554.5 Canis lupus familiaris zinc finger, ZZ-type with EF-hand domain 1 (ZZEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe06093 XM_005619866.2 Canis lupus familiaris zinc finger, ZZ-type with EF-hand domain 1 (ZZEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe288037
    Clone ID Related Accession (Same CDS sequence) XM_022418397.1
    Accession Version XM_022418397.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGACCA ACCCTGCCTT CGTCCAGACA GTGCTGCACA ACACGCAGAA GGCACTACAG 
    CACATGCCTC CACTTTCCCT CTCACCAGGA TCTACAGATG TCTCAACTTT CCTGTCTCCC
    AACGTTCTAG AAGAGGTGGA CAGCTTCCTC ATAAGGATAA CTAGCTGCTG TTCAACTCCA
    GAGGTGGAGC TGACTCTTCT GGCCTTTGCA CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG
    AGCTGTCTGT GTACCATTGC TGACCACCTG GACACACGCT ATGATGCCTC GTCCCTCATC
    TCCTCCATGG CATCAGTCAG GCAGACCCTG CTCCTTAAAT ACGGTAAACC TCTTCAGCTG
    ACCCTTCAGG CCTGTGATGT TAAAGGGAAA GAAGAGAAAT CAGGGCCCGA AAACCTCCTT
    GTCGAGCCGT GGACAAGAGA TGGGTTTCTT ACAGAGACGG GAAAGACCAG AGCAAGTACC
    ATTTTTTCTG CAGGAACTGA ATCTGCCTTC CAAGTTACAC AGATCAGGAT TATGGTTCGA
    CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG GTGTTTGCCT ATAACTCATC TTCTGAAAAA
    TTTCATGCCG AAGAACACTT CAAAAGGTTT GAAAAATATG ACAAGTGGAA GCTTCAGGAA
    TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT TGCTCAGCTG ATGACCTTGG CGAGGATGAC
    CCTATTGGCT GGTTTGAGCT AGAAGAAGAA TGGGATGAAG CAGATGTCAA GTTGCAGCAG
    TGCAGAGTTG CCAAATATTT GATGGTGAAG TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG
    CGCTTGGGAG TGCAAGGTCT CAGCATCAGC GGGTATGTCC GGCCAATGCG AGTGGAAGCG
    GAGCAGCACA TTGTCTGTGC CCACTGCAGA AAGGATGGGG AGGAGAGTGT CTGCGGGACC
    ACGCTACTCC TCAGGACGCT TCAGTTCACT CAGCAGCTTG CCCATGACCT GGTGCAGCAG
    AAGGAAAGTG GCTTAAAATA TAAATCTTTT CTGGACTTCG CGGGTCTTGA TTTGCAGATC
    TTCTGGAATT TTTATAGTAA ATTAAAGCAA AACCCGAGGG AAGAATGCAT CTGTGCCCAG
    ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC
    ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA
    CTCCACGCAC ACTTATGCGA CGTGGTGGAT AGGGTGGACG GAGACTCTGT GCCCATGGAA
    ACATTAAAAC AAGAAGTCAG GAACACCCTT CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT
    CGACAGAGCC GGAGGAGCCA TCTCTTCACC ATGATGAAGA ATGTCACCGA GCAGGAACAC
    AAGCAGTCCC AGCAGCTCAC TTTCCAGTCA CTATGCACCT ATTTTAGTGA CAAGGATCCA
    GGTGGTCTTC TGCTCTTGCC TGAGAAAGGT GACCTGGCTG ACATGAACAG CAGCGAAGTT
    CTGGCCGTCA TGAGCACCCT CCTGTCCGTT GCTGCTCGAG AGTGTGAGCT CCTGCTGCTT
    ACCAGGGCCC AGGGACAGCC CGGCTCCGTG CTCTTCTCCC TGTTCTGGGC CGTCCAGGGC
    AGCCTGCTCT CCTGGTGCTA CCTGCAGCTG AAGAGCACCG ACGCGGGAGC CCGGGGTCTC
    GCCGCCCACC TCATTGACCA GTATGTGGGA CAGTTTGTGG CAAGCACGAG AGTGATTCTG
    GAATCCCTTT TGTCACAGTA TAGTGGACAA ACCATTGTAG AGAAACTGTG TAATTCAGTG
    TTTTCGATGG CAGCTCGTCA ACTGGTGATC TTCCTGCTGG ACTTTTGCAC TTTAGACATT
    TCACACTGTG CACTCCTGAG AGAGTTCAGT ACACTGACAG AACTGTTAAA GCAGATCTGC
    GCGGACCCCA CAGGAGCGCC AGACAAGCTC GATGAAGACA CTTGGCAGCA GGAGTGTCCT
    GTGGTATTAC ACACCTGGAC CAAGGAGTCT GCCCACAACT ATGAGAACAA TTGCCATGAG
    GTGTCTGTCT TCATCAGCCC TGGGGCAACC TACTTTGAGG TAGAATTTGA TGAGCGCTGT
    GAGACTGAGA AGAGGTACGA TTATTTGGAG TTCACTGACG CTAGAGGCGG GAAAACACGC
    TATGACACCA AAGTTGGCAC TGAGAAGTGG CCCAGGAAAG TGATCTTCAA GGCTGGCCCC
    CGGCTGCAGT TCCTCTTTCA CTCTGACAGC AGTAACAATG AGTGGGGTTA CAAATTCACC
    GTCACCGCCT ATGGCCTGCC CGATGTGGCC GTGTCCTGGG CCCTGGATTT ACAGCTGCTC
    GTTTGCCGGC TGATGGGACG CCTCGCTGCC CAGTGCATGG CGCTCAAGTC TGCGCACCAG
    TTTGGAGGCG ACATGGCAGT GGCTCCAGCA AAAATGGCGT TGGTCCTGAA CTCTCCGCTG
    TGGAAACCTG TTTTCAGGCA TCAGTTGAGT CCAGAGTTGG GATTAGCAGT GAGCTGGCCC
    ACGCACCTGC ACCAGGATAG TAAGGAGGTC AAGAACATCC CCGATGATCC CTGCCACCAC
    TTTCTTCTTG AGTTTGCACA GTCAGACCCT GCCCAGAACT TCTGTGGGCC ATTTTCAGAA
    CTTTTCAAAG GATTCATACA GGCATGTAGA AAACAGGCCC CAAAGACAGA TATAGTTGCT
    GGTTCCACTA TTGATCAAGC TGTGAACGCC ACCTTTGCTG CTCTGGTGTG TCGTACTCCA
    GGCTTATATG AGAAGCTGCA AAAATATGTA AACAGTGGAG GCAACACAGC CCTGAGCGAG
    GACTTTGCAC AGGTGTATTG CTTGGCAGAT GGGATCCGAG TATGGATGCT GGAGATGAAG
    CAGAAGTCAT TGATGAACCT GGGGAATGAG GCAGAAGAAA AGCATGGCCC GGAAGCTGCC
    GAGGCAAACC CTGAGAGCCT GGCAAAAGAG TGTATTCAGA AGAGTCTTCT GTTACTACGA
    TTTTTGCCCA TGGGAGTAAG TTCAAAAGAA AGCTGTGACA AGCTGGTGGC TGCAGTAGAA
    ACAGATCACC TGCAGCCCTG CGACAGGTGC CCAAGGACGA GCTCTGTGGT GGAGGAGCAT
    TTCCAGGCCT CGGAGTCTCC CATGGACACA GGCACCCTTG CCGCAAGCAG TGGGGGTCCT
    AGCTCAGATG TGCCGCTGAA GCTGCTGCCC AGCGGTGGCC CCCCTGCCAC TGAGGTGTCC
    TTTGCCACTG CTGAAGAGCC CTCTTCACCT TCCGCTCCTG CCCGGCGGCC TCCCTTCACC
    CGAGGGCGAC TTCGACTGCT CTCCTTCCGG TCAGTGGAGG AGGCCAGACC CGTGCCCACC
    GTGAAGGAGA AATACCCCGT GCTGAAGGAT GTCCTGGACT TCATTAAGGA CCAGTCCCTC
    TCTCATGAGA GTGTCGTAAA GGTTCTTGCC TTGAGGAAAG CTCAAGCTCA GAGCATCCTG
    GAGGTCCTGA GGATCATTCA GTACTGCACG GAGTCTCTGG GACGGCCTCA TTGCTTCCAC
    CCGCCGTGTA TACTTTTCCT GATGGAGCTT CTCACCTGCC AGAAAGAGTT TACCAATTAT
    TTCGCACACA TGGAGGGCTG TGGTGCACGT CTGCGCAAAG AGATTCGAGA CACTTACTAC
    CAGTTTGTTC TGTTTTTGGT TAAAGCAGTT AAAGAATTTA GCAGCATGAA CGACAGGTCG
    CTGCTGCCTG CCTTGTCCTG TGTGCAGACA GCCCTGCTCC ACCTTCTGGA TATGGGCTGG
    GAACCCGGCG ATCTCGCTTT CTTTGTGGAC ATTGGGTTAC CACACCTCCT CATGAAACTG
    TCACAAGAGA ATATAAGCGT CCACGACAGC GTGATCAGCC AGTGCAGTGA AGAAGATGAG
    CTCGCGGATG CCAAGCAGAA TTCAGAATGG ATGGATGAGT GTCAGGATGG CATGTTTGAG
    GCCTGGTATG AAAAAATAGC CCAGGAAGAT CCAGAGAAGC AGAGGAAAAT GCACATGTTC
    ATTGCTCGCT ACTGCGACCT GCTAAACGTG GACATCTCCT GTGATGGCTG TGATGAGATT
    GCCCCCTGGC ACCGCTACCG GTGTCTGCAG TGCAGTGACA TGGATCTCTG CAAAACTTGC
    TTCCTAGGTG GGGTGAAGCC CGAGGGCCAT GGAGATGATC ATGAGATGGT CAGCATGGAG
    TTTACCTGTG ACCACTGCCA GGGTTTGATC ATCGGCCGGA GGATGAACTG CAACGTGTGT
    GATGACTTCG ATCTTTGCTA CGGGTGCTAC GCGGCAAAGA GATACTCTTA CGGCCACTTG
    CCCACCCACG GCGTCACAGC CCACCCGATG GTGACCATCC GGATCAGCGA CCGGCAGAGG
    CTTATCCAGC CGTACATCCA CAACTACGCC TGGCTGCTCT TTGCTGCCCT GGCTCTCTAC
    AGCGCCCACC TGGCCAGTGC AGAGGACGTG GATGGTGAGA AGCTGAATCC CCAGGACCGT
    GGCAGTGCCA GCCTGCTGCG GAGCCAGTGC ATGCAGCTTG TCGGGGACTG TCTGATGAAG
    GCTCACCAGG GAAAAGGTCT GAAAGCTCTA GCTCTCCTTG GTATATTGCC AGATGGTGAA
    CCTAGTCCAG AAAGTCGGGC CCCACCCGTC ACTGTGCCCA CCCACACGGC AGAGGAGGAG
    AGAGCTGTCT GGGGCCCTGA GGACCTGCCA CATACAGGCC TTTCTATGTG CTACAGTGGA
    AAGGAAGATG GTCCTAAGGA AGTCAGACCT TTAGATAGCA GGCAGAGAAG TAAGGCAGAT
    GAAGAAGGTG CATCCTTAAT GAATGGTCCT TCATGCCAGA CTCCAATCTC AGACACACCT
    GCAGATGGTA GCACCCCTGC AAGGCTTCCA GATGCTGAGA ATACAGTCAT GTTGTCTCAG
    GAGTCCATCG AGGAAAAGGC GGTTACTCCA AGTCCTGAGC AGGTGTTTGC TGAGTGCTCC
    CAGAAGAGGA TTTTAGGATT ACTAGCAGCC ATGTTACCTC CCTTAAAGTT GGACCCCACA
    GTCCCCGTGA TAGACCTGGA GCACGTCCTC CCACTCATGT TTCAGGTTGT CATCTCAAAC
    GTAGGCCACC TGAATGAGAC CTACCACCTC ACGCTGGGTC TTCTCGGCCA GCTCATCACC
    CGCCTCTTAC CAGCGGAGGT AGATGCTGCG GTGGTGAAAG TCCTCTCCGC CAAGCATACC
    TTGTTTGCAA CAGGGGACAG TTCTGTGGTG CCAGATGGCT GGAAGACCAC CCACCTGCTC
    TTTAGCCTGG GAGCCGTGTG TCTGGACAGC CGGGTGGGCT TGGACTGGGC ATGCTCCATG
    GCAGAGATCC TGCGGTCCCT CAACAGTGCC CCACTGTGGC GTGACGTCAT CGCCGCCTTC
    ACAGACCACT GTATCAGGCA GCTGCCTTTC CAGCTGAAGC ACACCAACAT CTTCACCCTG
    CTCGTGCTGG TCGGCTTCCC ACAGGTCCTC TGCGTGGGGA CCCGCTGTGT GTATGTGGAT
    CATGCCAATG AGCCACATAA TGTCATCATC TTGAAGCACT TCACGGAGAA GAACAGGGCT
    GTGATCGTTG ATGTCAAAAC CCGGAAAAGG AAAACAGTGA AGGACTACCA GCTGGTCCAG
    AAAGGACAGG AATGCAGCAG CTCTCAGGCC CAGCTGAGCC AGTACTCCCG GCACTTTGCC
    TTTATCGCCA GTCACCTTCT ACAGACCAGT ATGGACAGCC AATGTCCTGA GGCAGTAGAA
    GCAACATGGG TCCTGTCTCT GGCCCTGAAA GGACTGTATA AAACACTAAA GGCTCACAGT
    TTTGAAGAGA CCCACACCAC CTTCCTTCAG ACGGATTTGC TGAAGCTGCT GGTGAAAAAG
    TGCAGCAAAG GGACTGGTTT CAGTAAAACG TGGCTCCTCC GGGATCTGGA GATCCTGTCC
    ATCATGCTGT ACTCCTCCAA GAAGGAGAGC CATACCTCGG CTGAGCACAC AGCCCTGGAG
    CGAGATGAGA GAGGAGACCA AGAGGAGGAG GTGGATGGCA CAGTCAGCAG CCCTGGTGAG
    CCAGACCAAA AGAAGCTAGA CCCTCTGGAG AGCCTGGACG AGCCTACCAG AATATGTTTC
    TTGATGGCGC ACGATGCGCT CAATGCTCCT CTGCACATCC TCCGGGCCAT CTACGAGCTG
    CAGATGAAAA GGACGGATTC TTTCTTCCTG GAGGTTCAGA AGAGGTTTGA TGGGGATGAA
    CTCACCACAG ATGAGAGGAT TCGCACCCTG GCTCAGAGAT GGCAGCCCAG TAGGGGCCTG
    AGACTGGAGG AACAGAGCAC CAAAGCCGTG GATACAGACA TGATCATCTT ACCGTGTTTG
    TCCCGGCCTG CCCGCTGTGA CCAGGCCACG GCCGAATCCA ACCCCGTGAC CCAGAAGCTG
    ATCTCCAGCA CTGAGAGTGA ATTGCAGCAT AGCTACGCCA AACAGCGGCG CAGCAAGAGC
    ACCGCCCTCC TGCACAAGGA GCTGAATGGC AAGAGCAAGC GGGCCATCCG GGAGTACCTG
    TTCCGCGTGA ACGAAGCAAC AGCTGTCCTG TATGCCCGCC ATGTGCTGGC ATCCTTGCTC
    GCCGAGTGGC CGGGGCACGT ACCAGTGAGC GAGGACACCC TGGACCTCAG CGGCCCTGCC
    CACATGACCT ACATCTTGGA CATGTTCATG CAGCTGGAGG AGAAGCACCA GTGGGAGAAG
    ATCCTGCGGA AGGTGCTTCA GGGCTGCCGA GGGAGCATGC TGGGGACCAT GGCTCTGGCC
    GCCTGCCAGT TCATGGAGGA GCCGGGGATG GAGGTGCAAG TGAGAGAGTC AAAGCACCCG
    TATAACAACA ACACCAGCTT TGAGGATAAA GTCCACATCC CTGGTGCAAT CTACCTCTCG
    ATCAAATTTG ACTCGCAGTG TAGCACAGAG GAGGGCTGTG ACGAGCTAGC CATGTCCAGC
    AGTAGCGACT TCCAGCAGGA TCGACACACT TTCAGTGGGT CTCAGCAGAA ATGGAAGGAT
    TTTGAGCTTC CAGGGGATAC TCTATACTAC CGCTTCACCT CTGACATGAG CAACACGGAG
    TGGGGTTACA AGTTCACTGT GACAGCTGGA CACCTGGGCC GATTCCAGAC AGGCTTCGAG
    ATTTTGAAGC AGATGTTGTC AGAAGAAAGA GTTGTGCCAC ACCTCCCACT GGCCAAAATT
    TGGGAGTGGC TGGTGGGGGT GGCTTGTCGC CAGACTGGTC ATCAGCGATT GAAAGCCATC
    CACCTGCTTC TGAGGATTGT GCAATGCTGC ACCCACAGCG ACCTCTGTGA CCTTGCGCTG
    CTAAAGCCCC TGTGGCAGCT CTTCACTCAC ATGGAGTATA GCCTGTTCGA GGATGTGACC
    TCACCCGGCA TCCTCCTCCC CCTGCACCGT GCCCTCACTG AGCTCTTCTT CGTCACCGAG
    AACCGCGCCC AGGAGCTCGG CTTGCTGCAG GAGTATCTGC TGGCCTTGAC CACGGACGAC
    CACCTTCTCC GCTGCGCGGC ACAGGCCCTG CAGAATGTCG CTGCTATCAG CCTGGCCATC
    AACTACCCAA ACAAGGCAAC CCGCCTCTGG AACGTTGAGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274105.1
    CDS221..7885
    Translation

    Target ORF information:

    RefSeq Version XM_022418397.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418397.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGAGACCA ACCCTGCCTT CGTCCAGACA GTGCTGCACA ACACGCAGAA GGCACTACAG 
    CACATGCCTC CACTTTCCCT CTCACCAGGA TCTACAGATG TCTCAACTTT CCTGTCTCCC
    AACGTTCTAG AAGAGGTGGA CAGCTTCCTC ATAAGGATAA CTAGCTGCTG TTCAACTCCA
    GAGGTGGAGC TGACTCTTCT GGCCTTTGCA CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG
    AGCTGTCTGT GTACCATTGC TGACCACCTG GACACACGCT ATGATGCCTC GTCCCTCATC
    TCCTCCATGG CATCAGTCAG GCAGACCCTG CTCCTTAAAT ACGGTAAACC TCTTCAGCTG
    ACCCTTCAGG CCTGTGATGT TAAAGGGAAA GAAGAGAAAT CAGGGCCCGA AAACCTCCTT
    GTCGAGCCGT GGACAAGAGA TGGGTTTCTT ACAGAGACGG GAAAGACCAG AGCAAGTACC
    ATTTTTTCTG CAGGAACTGA ATCTGCCTTC CAAGTTACAC AGATCAGGAT TATGGTTCGA
    CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG GTGTTTGCCT ATAACTCATC TTCTGAAAAA
    TTTCATGCCG AAGAACACTT CAAAAGGTTT GAAAAATATG ACAAGTGGAA GCTTCAGGAA
    TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT TGCTCAGCTG ATGACCTTGG CGAGGATGAC
    CCTATTGGCT GGTTTGAGCT AGAAGAAGAA TGGGATGAAG CAGATGTCAA GTTGCAGCAG
    TGCAGAGTTG CCAAATATTT GATGGTGAAG TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG
    CGCTTGGGAG TGCAAGGTCT CAGCATCAGC GGGTATGTCC GGCCAATGCG AGTGGAAGCG
    GAGCAGCACA TTGTCTGTGC CCACTGCAGA AAGGATGGGG AGGAGAGTGT CTGCGGGACC
    ACGCTACTCC TCAGGACGCT TCAGTTCACT CAGCAGCTTG CCCATGACCT GGTGCAGCAG
    AAGGAAAGTG GCTTAAAATA TAAATCTTTT CTGGACTTCG CGGGTCTTGA TTTGCAGATC
    TTCTGGAATT TTTATAGTAA ATTAAAGCAA AACCCGAGGG AAGAATGCAT CTGTGCCCAG
    ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC
    ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA
    CTCCACGCAC ACTTATGCGA CGTGGTGGAT AGGGTGGACG GAGACTCTGT GCCCATGGAA
    ACATTAAAAC AAGAAGTCAG GAACACCCTT CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT
    CGACAGAGCC GGAGGAGCCA TCTCTTCACC ATGATGAAGA ATGTCACCGA GCAGGAACAC
    AAGCAGTCCC AGCAGCTCAC TTTCCAGTCA CTATGCACCT ATTTTAGTGA CAAGGATCCA
    GGTGGTCTTC TGCTCTTGCC TGAGAAAGGT GACCTGGCTG ACATGAACAG CAGCGAAGTT
    CTGGCCGTCA TGAGCACCCT CCTGTCCGTT GCTGCTCGAG AGTGTGAGCT CCTGCTGCTT
    ACCAGGGCCC AGGGACAGCC CGGCTCCGTG CTCTTCTCCC TGTTCTGGGC CGTCCAGGGC
    AGCCTGCTCT CCTGGTGCTA CCTGCAGCTG AAGAGCACCG ACGCGGGAGC CCGGGGTCTC
    GCCGCCCACC TCATTGACCA GTATGTGGGA CAGTTTGTGG CAAGCACGAG AGTGATTCTG
    GAATCCCTTT TGTCACAGTA TAGTGGACAA ACCATTGTAG AGAAACTGTG TAATTCAGTG
    TTTTCGATGG CAGCTCGTCA ACTGGTGATC TTCCTGCTGG ACTTTTGCAC TTTAGACATT
    TCACACTGTG CACTCCTGAG AGAGTTCAGT ACACTGACAG AACTGTTAAA GCAGATCTGC
    GCGGACCCCA CAGGAGCGCC AGACAAGCTC GATGAAGACA CTTGGCAGCA GGAGTGTCCT
    GTGGTATTAC ACACCTGGAC CAAGGAGTCT GCCCACAACT ATGAGAACAA TTGCCATGAG
    GTGTCTGTCT TCATCAGCCC TGGGGCAACC TACTTTGAGG TAGAATTTGA TGAGCGCTGT
    GAGACTGAGA AGAGGTACGA TTATTTGGAG TTCACTGACG CTAGAGGCGG GAAAACACGC
    TATGACACCA AAGTTGGCAC TGAGAAGTGG CCCAGGAAAG TGATCTTCAA GGCTGGCCCC
    CGGCTGCAGT TCCTCTTTCA CTCTGACAGC AGTAACAATG AGTGGGGTTA CAAATTCACC
    GTCACCGCCT ATGGCCTGCC CGATGTGGCC GTGTCCTGGG CCCTGGATTT ACAGCTGCTC
    GTTTGCCGGC TGATGGGACG CCTCGCTGCC CAGTGCATGG CGCTCAAGTC TGCGCACCAG
    TTTGGAGGCG ACATGGCAGT GGCTCCAGCA AAAATGGCGT TGGTCCTGAA CTCTCCGCTG
    TGGAAACCTG TTTTCAGGCA TCAGTTGAGT CCAGAGTTGG GATTAGCAGT GAGCTGGCCC
    ACGCACCTGC ACCAGGATAG TAAGGAGGTC AAGAACATCC CCGATGATCC CTGCCACCAC
    TTTCTTCTTG AGTTTGCACA GTCAGACCCT GCCCAGAACT TCTGTGGGCC ATTTTCAGAA
    CTTTTCAAAG GATTCATACA GGCATGTAGA AAACAGGCCC CAAAGACAGA TATAGTTGCT
    GGTTCCACTA TTGATCAAGC TGTGAACGCC ACCTTTGCTG CTCTGGTGTG TCGTACTCCA
    GGCTTATATG AGAAGCTGCA AAAATATGTA AACAGTGGAG GCAACACAGC CCTGAGCGAG
    GACTTTGCAC AGGTGTATTG CTTGGCAGAT GGGATCCGAG TATGGATGCT GGAGATGAAG
    CAGAAGTCAT TGATGAACCT GGGGAATGAG GCAGAAGAAA AGCATGGCCC GGAAGCTGCC
    GAGGCAAACC CTGAGAGCCT GGCAAAAGAG TGTATTCAGA AGAGTCTTCT GTTACTACGA
    TTTTTGCCCA TGGGAGTAAG TTCAAAAGAA AGCTGTGACA AGCTGGTGGC TGCAGTAGAA
    ACAGATCACC TGCAGCCCTG CGACAGGTGC CCAAGGACGA GCTCTGTGGT GGAGGAGCAT
    TTCCAGGCCT CGGAGTCTCC CATGGACACA GGCACCCTTG CCGCAAGCAG TGGGGGTCCT
    AGCTCAGATG TGCCGCTGAA GCTGCTGCCC AGCGGTGGCC CCCCTGCCAC TGAGGTGTCC
    TTTGCCACTG CTGAAGAGCC CTCTTCACCT TCCGCTCCTG CCCGGCGGCC TCCCTTCACC
    CGAGGGCGAC TTCGACTGCT CTCCTTCCGG TCAGTGGAGG AGGCCAGACC CGTGCCCACC
    GTGAAGGAGA AATACCCCGT GCTGAAGGAT GTCCTGGACT TCATTAAGGA CCAGTCCCTC
    TCTCATGAGA GTGTCGTAAA GGTTCTTGCC TTGAGGAAAG CTCAAGCTCA GAGCATCCTG
    GAGGTCCTGA GGATCATTCA GTACTGCACG GAGTCTCTGG GACGGCCTCA TTGCTTCCAC
    CCGCCGTGTA TACTTTTCCT GATGGAGCTT CTCACCTGCC AGAAAGAGTT TACCAATTAT
    TTCGCACACA TGGAGGGCTG TGGTGCACGT CTGCGCAAAG AGATTCGAGA CACTTACTAC
    CAGTTTGTTC TGTTTTTGGT TAAAGCAGTT AAAGAATTTA GCAGCATGAA CGACAGGTCG
    CTGCTGCCTG CCTTGTCCTG TGTGCAGACA GCCCTGCTCC ACCTTCTGGA TATGGGCTGG
    GAACCCGGCG ATCTCGCTTT CTTTGTGGAC ATTGGGTTAC CACACCTCCT CATGAAACTG
    TCACAAGAGA ATATAAGCGT CCACGACAGC GTGATCAGCC AGTGCAGTGA AGAAGATGAG
    CTCGCGGATG CCAAGCAGAA TTCAGAATGG ATGGATGAGT GTCAGGATGG CATGTTTGAG
    GCCTGGTATG AAAAAATAGC CCAGGAAGAT CCAGAGAAGC AGAGGAAAAT GCACATGTTC
    ATTGCTCGCT ACTGCGACCT GCTAAACGTG GACATCTCCT GTGATGGCTG TGATGAGATT
    GCCCCCTGGC ACCGCTACCG GTGTCTGCAG TGCAGTGACA TGGATCTCTG CAAAACTTGC
    TTCCTAGGTG GGGTGAAGCC CGAGGGCCAT GGAGATGATC ATGAGATGGT CAGCATGGAG
    TTTACCTGTG ACCACTGCCA GGGTTTGATC ATCGGCCGGA GGATGAACTG CAACGTGTGT
    GATGACTTCG ATCTTTGCTA CGGGTGCTAC GCGGCAAAGA GATACTCTTA CGGCCACTTG
    CCCACCCACG GCGTCACAGC CCACCCGATG GTGACCATCC GGATCAGCGA CCGGCAGAGG
    CTTATCCAGC CGTACATCCA CAACTACGCC TGGCTGCTCT TTGCTGCCCT GGCTCTCTAC
    AGCGCCCACC TGGCCAGTGC AGAGGACGTG GATGGTGAGA AGCTGAATCC CCAGGACCGT
    GGCAGTGCCA GCCTGCTGCG GAGCCAGTGC ATGCAGCTTG TCGGGGACTG TCTGATGAAG
    GCTCACCAGG GAAAAGGTCT GAAAGCTCTA GCTCTCCTTG GTATATTGCC AGATGGTGAA
    CCTAGTCCAG AAAGTCGGGC CCCACCCGTC ACTGTGCCCA CCCACACGGC AGAGGAGGAG
    AGAGCTGTCT GGGGCCCTGA GGACCTGCCA CATACAGGCC TTTCTATGTG CTACAGTGGA
    AAGGAAGATG GTCCTAAGGA AGTCAGACCT TTAGATAGCA GGCAGAGAAG TAAGGCAGAT
    GAAGAAGGTG CATCCTTAAT GAATGGTCCT TCATGCCAGA CTCCAATCTC AGACACACCT
    GCAGATGGTA GCACCCCTGC AAGGCTTCCA GATGCTGAGA ATACAGTCAT GTTGTCTCAG
    GAGTCCATCG AGGAAAAGGC GGTTACTCCA AGTCCTGAGC AGGTGTTTGC TGAGTGCTCC
    CAGAAGAGGA TTTTAGGATT ACTAGCAGCC ATGTTACCTC CCTTAAAGTT GGACCCCACA
    GTCCCCGTGA TAGACCTGGA GCACGTCCTC CCACTCATGT TTCAGGTTGT CATCTCAAAC
    GTAGGCCACC TGAATGAGAC CTACCACCTC ACGCTGGGTC TTCTCGGCCA GCTCATCACC
    CGCCTCTTAC CAGCGGAGGT AGATGCTGCG GTGGTGAAAG TCCTCTCCGC CAAGCATACC
    TTGTTTGCAA CAGGGGACAG TTCTGTGGTG CCAGATGGCT GGAAGACCAC CCACCTGCTC
    TTTAGCCTGG GAGCCGTGTG TCTGGACAGC CGGGTGGGCT TGGACTGGGC ATGCTCCATG
    GCAGAGATCC TGCGGTCCCT CAACAGTGCC CCACTGTGGC GTGACGTCAT CGCCGCCTTC
    ACAGACCACT GTATCAGGCA GCTGCCTTTC CAGCTGAAGC ACACCAACAT CTTCACCCTG
    CTCGTGCTGG TCGGCTTCCC ACAGGTCCTC TGCGTGGGGA CCCGCTGTGT GTATGTGGAT
    CATGCCAATG AGCCACATAA TGTCATCATC TTGAAGCACT TCACGGAGAA GAACAGGGCT
    GTGATCGTTG ATGTCAAAAC CCGGAAAAGG AAAACAGTGA AGGACTACCA GCTGGTCCAG
    AAAGGACAGG AATGCAGCAG CTCTCAGGCC CAGCTGAGCC AGTACTCCCG GCACTTTGCC
    TTTATCGCCA GTCACCTTCT ACAGACCAGT ATGGACAGCC AATGTCCTGA GGCAGTAGAA
    GCAACATGGG TCCTGTCTCT GGCCCTGAAA GGACTGTATA AAACACTAAA GGCTCACAGT
    TTTGAAGAGA CCCACACCAC CTTCCTTCAG ACGGATTTGC TGAAGCTGCT GGTGAAAAAG
    TGCAGCAAAG GGACTGGTTT CAGTAAAACG TGGCTCCTCC GGGATCTGGA GATCCTGTCC
    ATCATGCTGT ACTCCTCCAA GAAGGAGAGC CATACCTCGG CTGAGCACAC AGCCCTGGAG
    CGAGATGAGA GAGGAGACCA AGAGGAGGAG GTGGATGGCA CAGTCAGCAG CCCTGGTGAG
    CCAGACCAAA AGAAGCTAGA CCCTCTGGAG AGCCTGGACG AGCCTACCAG AATATGTTTC
    TTGATGGCGC ACGATGCGCT CAATGCTCCT CTGCACATCC TCCGGGCCAT CTACGAGCTG
    CAGATGAAAA GGACGGATTC TTTCTTCCTG GAGGTTCAGA AGAGGTTTGA TGGGGATGAA
    CTCACCACAG ATGAGAGGAT TCGCACCCTG GCTCAGAGAT GGCAGCCCAG TAGGGGCCTG
    AGACTGGAGG AACAGAGCAC CAAAGCCGTG GATACAGACA TGATCATCTT ACCGTGTTTG
    TCCCGGCCTG CCCGCTGTGA CCAGGCCACG GCCGAATCCA ACCCCGTGAC CCAGAAGCTG
    ATCTCCAGCA CTGAGAGTGA ATTGCAGCAT AGCTACGCCA AACAGCGGCG CAGCAAGAGC
    ACCGCCCTCC TGCACAAGGA GCTGAATGGC AAGAGCAAGC GGGCCATCCG GGAGTACCTG
    TTCCGCGTGA ACGAAGCAAC AGCTGTCCTG TATGCCCGCC ATGTGCTGGC ATCCTTGCTC
    GCCGAGTGGC CGGGGCACGT ACCAGTGAGC GAGGACACCC TGGACCTCAG CGGCCCTGCC
    CACATGACCT ACATCTTGGA CATGTTCATG CAGCTGGAGG AGAAGCACCA GTGGGAGAAG
    ATCCTGCGGA AGGTGCTTCA GGGCTGCCGA GGGAGCATGC TGGGGACCAT GGCTCTGGCC
    GCCTGCCAGT TCATGGAGGA GCCGGGGATG GAGGTGCAAG TGAGAGAGTC AAAGCACCCG
    TATAACAACA ACACCAGCTT TGAGGATAAA GTCCACATCC CTGGTGCAAT CTACCTCTCG
    ATCAAATTTG ACTCGCAGTG TAGCACAGAG GAGGGCTGTG ACGAGCTAGC CATGTCCAGC
    AGTAGCGACT TCCAGCAGGA TCGACACACT TTCAGTGGGT CTCAGCAGAA ATGGAAGGAT
    TTTGAGCTTC CAGGGGATAC TCTATACTAC CGCTTCACCT CTGACATGAG CAACACGGAG
    TGGGGTTACA AGTTCACTGT GACAGCTGGA CACCTGGGCC GATTCCAGAC AGGCTTCGAG
    ATTTTGAAGC AGATGTTGTC AGAAGAAAGA GTTGTGCCAC ACCTCCCACT GGCCAAAATT
    TGGGAGTGGC TGGTGGGGGT GGCTTGTCGC CAGACTGGTC ATCAGCGATT GAAAGCCATC
    CACCTGCTTC TGAGGATTGT GCAATGCTGC ACCCACAGCG ACCTCTGTGA CCTTGCGCTG
    CTAAAGCCCC TGTGGCAGCT CTTCACTCAC ATGGAGTATA GCCTGTTCGA GGATGTGACC
    TCACCCGGCA TCCTCCTCCC CCTGCACCGT GCCCTCACTG AGCTCTTCTT CGTCACCGAG
    AACCGCGCCC AGGAGCTCGG CTTGCTGCAG GAGTATCTGC TGGCCTTGAC CACGGACGAC
    CACCTTCTCC GCTGCGCGGC ACAGGCCCTG CAGAATGTCG CTGCTATCAG CCTGGCCATC
    AACTACCCAA ACAAGGCAAC CCGCCTCTGG AACGTTGAGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06092
    Clone ID Related Accession (Same CDS sequence) XM_546554.5 , XM_546554.6
    Accession Version XM_546554.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546554.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1141
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    1321
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    1501
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    1981
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    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAATG TCACCGAGCA GGAACACAAG CAGTCCCAGC AGCTCACTTT CCAGTCACTA
    TGCACCTATT TTAGTGACAA GGATCCAGGT GGTCTTCTGC TCTTGCCTGA GAAAGGTGAC
    CTGGCTGACA TGAACAGCAG CGAAGTTCTG GCCGTCATGA GCACCCTCCT GTCCGTTGCT
    GCTCGAGAGT GTGAGCTCCT GCTGCTTACC AGGGCCCAGG GACAGCCCGG CTCCGTGCTC
    TTCTCCCTGT TCTGGGCCGT CCAGGGCAGC CTGCTCTCCT GGTGCTACCT GCAGCTGAAG
    AGCACCGACG CGGGAGCCCG GGGTCTCGCC GCCCACCTCA TTGACCAGTA TGTGGGACAG
    TTTGTGGCAA GCACGAGAGT GATTCTGGAA TCCCTTTTGT CACAGTATAG TGGACAAACC
    ATTGTAGAGA AACTGTGTAA TTCAGTGTTT TCGATGGCAG CTCGTCAACT GGTGATCTTC
    CTGCTGGACT TTTGCACTTT AGACATTTCA CACTGTGCAC TCCTGAGAGA GTTCAGTACA
    CTGACAGAAC TGTTAAAGCA GATCTGCGCG GACCCCACAG GAGCGCCAGA CAAGCTCGAT
    GAAGACACTT GGCAGCAGGA GTGTCCTGTG GTATTACACA CCTGGACCAA GGAGTCTGCC
    CACAACTATG AGAACAATTG CCATGAGGTG TCTGTCTTCA TCAGCCCTGG GGCAACCTAC
    TTTGAGGTAG AATTTGATGA GCGCTGTGAG ACTGAGAAGA GGTACGATTA TTTGGAGTTC
    ACTGACGCTA GAGGCGGGAA AACACGCTAT GACACCAAAG TTGGCACTGA GAAGTGGCCC
    AGGAAAGTGA TCTTCAAGGC TGGCCCCCGG CTGCAGTTCC TCTTTCACTC TGACAGCAGT
    AACAATGAGT GGGGTTACAA ATTCACCGTC ACCGCCTATG GCCTGCCCGA TGTGGCCGTG
    TCCTGGGCCC TGGATTTACA GCTGCTCGTT TGCCGGCTGA TGGGACGCCT CGCTGCCCAG
    TGCATGGCGC TCAAGTCTGC GCACCAGTTT GGAGGCGACA TGGCAGTGGC TCCAGCAAAA
    ATGGCGTTGG TCCTGAACTC TCCGCTGTGG AAACCTGTTT TCAGGCATCA GTTGAGTCCA
    GAGTTGGGAT TAGCAGTGAG CTGGCCCACG CACCTGCACC AGGATAGTAA GGAGGTCAAG
    AACATCCCCG ATGATCCCTG CCACCACTTT CTTCTTGAGT TTGCACAGTC AGACCCTGCC
    CAGAACTTCT GTGGGCCATT TTCAGAACTT TTCAAAGGAT TCATACAGGC ATGTAGAAAA
    CAGGCCCCAA AGACAGATAT AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC
    TTTGCTGCTC TGGTGTGTCG TACTCCAGGC TTATATGAGA AGCTGCAAAA ATATGTAAAC
    AGTGGAGGCA ACACAGCCCT GAGCGAGGAC TTTGCACAGG TGTATTGCTT GGCAGATGGG
    ATCCGAGTAT GGATGCTGGA GATGAAGCAG AAGTCATTGA TGAACCTGGG GAATGAGGCA
    GAAGAAAAGC ATGGCCCGGA AGCTGCCGAG GCAAACCCTG AGAGCCTGGC AAAAGAGTGT
    ATTCAGAAGA GTCTTCTGTT ACTACGATTT TTGCCCATGG GAGTAAGTTC AAAAGAAAGC
    TGTGACAAGC TGGTGGCTGC AGTAGAAACA GATCACCTGC AGCCCTGCGA CAGGTGCCCA
    AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGG AGTCTCCCAT GGACACAGGC
    ACCCTTGCCG CAAGCAGTGG GGGTCCTAGC TCAGATGTGC CGCTGAAGCT GCTGCCCAGC
    GGTGGCCCCC CTGCCACTGA GGTGTCCTTT GCCACTGCTG AAGAGCCCTC TTCACCTTCC
    GCTCCTGCCC GGCGGCCTCC CTTCACCCGA GGGCGACTTC GACTGCTCTC CTTCCGGTCA
    GTGGAGGAGG CCAGACCCGT GCCCACCGTG AAGGAGAAAT ACCCCGTGCT GAAGGATGTC
    CTGGACTTCA TTAAGGACCA GTCCCTCTCT CATGAGAGTG TCGTAAAGGT TCTTGCCTTG
    AGGAAAGCTC AAGCTCAGAG CATCCTGGAG GTCCTGAGGA TCATTCAGTA CTGCACGGAG
    TCTCTGGGAC GGCCTCATTG CTTCCACCCG CCGTGTATAC TTTTCCTGAT GGAGCTTCTC
    ACCTGCCAGA AAGAGTTTAC CAATTATTTC GCACACATGG AGGGCTGTGG TGCACGTCTG
    CGCAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTTAA AGCAGTTAAA
    GAATTTAGCA GCATGAACGA CAGGTCGCTG CTGCCTGCCT TGTCCTGTGT GCAGACAGCC
    CTGCTCCACC TTCTGGATAT GGGCTGGGAA CCCGGCGATC TCGCTTTCTT TGTGGACATT
    GGGTTACCAC ACCTCCTCAT GAAACTGTCA CAAGAGAATA TAAGCGTCCA CGACAGCGTG
    ATCAGCCAGT GCAGTGAAGA AGATGAGCTC GCGGATGCCA AGCAGAATTC AGAATGGATG
    GATGAGTGTC AGGATGGCAT GTTTGAGGCC TGGTATGAAA AAATAGCCCA GGAAGATCCA
    GAGAAGCAGA GGAAAATGCA CATGTTCATT GCTCGCTACT GCGACCTGCT AAACGTGGAC
    ATCTCCTGTG ATGGCTGTGA TGAGATTGCC CCCTGGCACC GCTACCGGTG TCTGCAGTGC
    AGTGACATGG ATCTCTGCAA AACTTGCTTC CTAGGTGGGG TGAAGCCCGA GGGCCATGGA
    GATGATCATG AGATGGTCAG CATGGAGTTT ACCTGTGACC ACTGCCAGGG TTTGATCATC
    GGCCGGAGGA TGAACTGCAA CGTGTGTGAT GACTTCGATC TTTGCTACGG GTGCTACGCG
    GCAAAGAGAT ACTCTTACGG CCACTTGCCC ACCCACGGCG TCACAGCCCA CCCGATGGTG
    ACCATCCGGA TCAGCGACCG GCAGAGGCTT ATCCAGCCGT ACATCCACAA CTACGCCTGG
    CTGCTCTTTG CTGCCCTGGC TCTCTACAGC GCCCACCTGG CCAGTGCAGA GGACGTGGAT
    GGTGAGAAGC TGAATCCCCA GGACCGTGGC AGTGCCAGCC TGCTGCGGAG CCAGTGCATG
    CAGCTTGTCG GGGACTGTCT GATGAAGGCT CACCAGGGAA AAGGTCTGAA AGCTCTAGCT
    CTCCTTGGTA TATTGCCAGA TGGTGAACCT AGTCCAGAAA GTCGGGCCCC ACCCGTCACT
    GTGCCCACCC ACACGGCAGA GGAGGAGAGA GCTGTCTGGG GCCCTGAGGA CCTGCCACAT
    ACAGGCCTTT CTATGTGCTA CAGTGGAAAG GAAGATGGTC CTAAGGAAGT CAGACCTTTA
    GATAGCAGGC AGAGAAGTAA GGCAGATGAA GAAGGTGCAT CCTTAATGAA TGGTCCTTCA
    TGCCAGACTC CAATCTCAGA CACACCTGCA GATGGTAGCA CCCCTGCAAG GCTTCCAGAT
    GCTGAGAATA CAGTCATGTT GTCTCAGGAG TCCATCGAGG AAAAGGCGGT TACTCCAAGT
    CCTGAGCAGG TGTTTGCTGA GTGCTCCCAG AAGAGGATTT TAGGATTACT AGCAGCCATG
    TTACCTCCCT TAAAGTTGGA CCCCACAGTC CCCGTGATAG ACCTGGAGCA CGTCCTCCCA
    CTCATGTTTC AGGTTGTCAT CTCAAACGTA GGCCACCTGA ATGAGACCTA CCACCTCACG
    CTGGGTCTTC TCGGCCAGCT CATCACCCGC CTCTTACCAG CGGAGGTAGA TGCTGCGGTG
    GTGAAAGTCC TCTCCGCCAA GCATACCTTG TTTGCAACAG GGGACAGTTC TGTGGTGCCA
    GATGGCTGGA AGACCACCCA CCTGCTCTTT AGCCTGGGAG CCGTGTGTCT GGACAGCCGG
    GTGGGCTTGG ACTGGGCATG CTCCATGGCA GAGATCCTGC GGTCCCTCAA CAGTGCCCCA
    CTGTGGCGTG ACGTCATCGC CGCCTTCACA GACCACTGTA TCAGGCAGCT GCCTTTCCAG
    CTGAAGCACA CCAACATCTT CACCCTGCTC GTGCTGGTCG GCTTCCCACA GGTCCTCTGC
    GTGGGGACCC GCTGTGTGTA TGTGGATCAT GCCAATGAGC CACATAATGT CATCATCTTG
    AAGCACTTCA CGGAGAAGAA CAGGGCTGTG ATCGTTGATG TCAAAACCCG GAAAAGGAAA
    ACAGTGAAGG ACTACCAGCT GGTCCAGAAA GGACAGGAAT GCAGCAGCTC TCAGGCCCAG
    CTGAGCCAGT ACTCCCGGCA CTTTGCCTTT ATCGCCAGTC ACCTTCTACA GACCAGTATG
    GACAGCCAAT GTCCTGAGGC AGTAGAAGCA ACATGGGTCC TGTCTCTGGC CCTGAAAGGA
    CTGTATAAAA CACTAAAGGC TCACAGTTTT GAAGAGACCC ACACCACCTT CCTTCAGACG
    GATTTGCTGA AGCTGCTGGT GAAAAAGTGC AGCAAAGGGA CTGGTTTCAG TAAAACGTGG
    CTCCTCCGGG ATCTGGAGAT CCTGTCCATC ATGCTGTACT CCTCCAAGAA GGAGAGCCAT
    ACCTCGGCTG AGCACACAGC CCTGGAGCGA GATGAGAGAG GAGACCAAGA GGAGGAGGTG
    GATGGCACAG TCAGCAGCCC TGGTGAGCCA GACCAAAAGA AGCTAGACCC TCTGGAGAGC
    CTGGACGAGC CTACCAGAAT ATGTTTCTTG ATGGCGCACG ATGCGCTCAA TGCTCCTCTG
    CACATCCTCC GGGCCATCTA CGAGCTGCAG ATGAAAAGGA CGGATTCTTT CTTCCTGGAG
    GTTCAGAAGA GGTTTGATGG GGATGAACTC ACCACAGATG AGAGGATTCG CACCCTGGCT
    CAGAGATGGC AGCCCAGTAG GGGCCTGAGA CTGGAGGAAC AGAGCACCAA AGCCGTGGAT
    ACAGACATGA TCATCTTACC GTGTTTGTCC CGGCCTGCCC GCTGTGACCA GGCCACGGCC
    GAATCCAACC CCGTGACCCA GAAGCTGATC TCCAGCACTG AGAGTGAATT GCAGCATAGC
    TACGCCAAAC AGCGGCGCAG CAAGAGCACC GCCCTCCTGC ACAAGGAGCT GAATGGCAAG
    AGCAAGCGGG CCATCCGGGA GTACCTGTTC CGCGTGAACG AAGCAACAGC TGTCCTGTAT
    GCCCGCCATG TGCTGGCATC CTTGCTCGCC GAGTGGCCGG GGCACGTACC AGTGAGCGAG
    GACACCCTGG ACCTCAGCGG CCCTGCCCAC ATGACCTACA TCTTGGACAT GTTCATGCAG
    CTGGAGGAGA AGCACCAGTG GGAGAAGATC CTGCGGAAGG TGCTTCAGGG CTGCCGAGGG
    AGCATGCTGG GGACCATGGC TCTGGCCGCC TGCCAGTTCA TGGAGGAGCC GGGGATGGAG
    GTGCAAGTGA GAGAGTCAAA GCACCCGTAT AACAACAACA CCAGCTTTGA GGATAAAGTC
    CACATCCCTG GTGCAATCTA CCTCTCGATC AAATTTGACT CGCAGTGTAG CACAGAGGAG
    GGCTGTGACG AGCTAGCCAT GTCCAGCAGT AGCGACTTCC AGCAGGATCG ACACACTTTC
    AGTGGGTCTC AGCAGAAATG GAAGGATTTT GAGCTTCCAG GGGATACTCT ATACTACCGC
    TTCACCTCTG ACATGAGCAA CACGGAGTGG GGTTACAAGT TCACTGTGAC AGCTGGACAC
    CTGGGCCGAT TCCAGACAGG CTTCGAGATT TTGAAGCAGA TGTTGTCAGA AGAAAGAGTT
    GTGCCACACC TCCCACTGGC CAAAATTTGG GAGTGGCTGG TGGGGGTGGC TTGTCGCCAG
    ACTGGTCATC AGCGATTGAA AGCCATCCAC CTGCTTCTGA GGATTGTGCA ATGCTGCACC
    CACAGCGACC TCTGTGACCT TGCGCTGCTA AAGCCCCTGT GGCAGCTCTT CACTCACATG
    GAGTATAGCC TGTTCGAGGA TGTGACCTCA CCCGGCATCC TCCTCCCCCT GCACCGTGCC
    CTCACTGAGC TCTTCTTCGT CACCGAGAAC CGCGCCCAGG AGCTCGGCTT GCTGCAGGAG
    TATCTGCTGG CCTTGACCAC GGACGACCAC CTTCTCCGCT GCGCGGCACA GGCCCTGCAG
    AATGTCGCTG CTATCAGCCT GGCCATCAAC TACCCAAACA AGGCAACCCG CCTCTGGAAC
    GTTGAGTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546554.3
    CDS173..9064
    Translation

    Target ORF information:

    RefSeq Version XM_546554.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris zinc finger, ZZ-type with EF-hand domain 1 (ZZEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546554.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAATG TCACCGAGCA GGAACACAAG CAGTCCCAGC AGCTCACTTT CCAGTCACTA
    TGCACCTATT TTAGTGACAA GGATCCAGGT GGTCTTCTGC TCTTGCCTGA GAAAGGTGAC
    CTGGCTGACA TGAACAGCAG CGAAGTTCTG GCCGTCATGA GCACCCTCCT GTCCGTTGCT
    GCTCGAGAGT GTGAGCTCCT GCTGCTTACC AGGGCCCAGG GACAGCCCGG CTCCGTGCTC
    TTCTCCCTGT TCTGGGCCGT CCAGGGCAGC CTGCTCTCCT GGTGCTACCT GCAGCTGAAG
    AGCACCGACG CGGGAGCCCG GGGTCTCGCC GCCCACCTCA TTGACCAGTA TGTGGGACAG
    TTTGTGGCAA GCACGAGAGT GATTCTGGAA TCCCTTTTGT CACAGTATAG TGGACAAACC
    ATTGTAGAGA AACTGTGTAA TTCAGTGTTT TCGATGGCAG CTCGTCAACT GGTGATCTTC
    CTGCTGGACT TTTGCACTTT AGACATTTCA CACTGTGCAC TCCTGAGAGA GTTCAGTACA
    CTGACAGAAC TGTTAAAGCA GATCTGCGCG GACCCCACAG GAGCGCCAGA CAAGCTCGAT
    GAAGACACTT GGCAGCAGGA GTGTCCTGTG GTATTACACA CCTGGACCAA GGAGTCTGCC
    CACAACTATG AGAACAATTG CCATGAGGTG TCTGTCTTCA TCAGCCCTGG GGCAACCTAC
    TTTGAGGTAG AATTTGATGA GCGCTGTGAG ACTGAGAAGA GGTACGATTA TTTGGAGTTC
    ACTGACGCTA GAGGCGGGAA AACACGCTAT GACACCAAAG TTGGCACTGA GAAGTGGCCC
    AGGAAAGTGA TCTTCAAGGC TGGCCCCCGG CTGCAGTTCC TCTTTCACTC TGACAGCAGT
    AACAATGAGT GGGGTTACAA ATTCACCGTC ACCGCCTATG GCCTGCCCGA TGTGGCCGTG
    TCCTGGGCCC TGGATTTACA GCTGCTCGTT TGCCGGCTGA TGGGACGCCT CGCTGCCCAG
    TGCATGGCGC TCAAGTCTGC GCACCAGTTT GGAGGCGACA TGGCAGTGGC TCCAGCAAAA
    ATGGCGTTGG TCCTGAACTC TCCGCTGTGG AAACCTGTTT TCAGGCATCA GTTGAGTCCA
    GAGTTGGGAT TAGCAGTGAG CTGGCCCACG CACCTGCACC AGGATAGTAA GGAGGTCAAG
    AACATCCCCG ATGATCCCTG CCACCACTTT CTTCTTGAGT TTGCACAGTC AGACCCTGCC
    CAGAACTTCT GTGGGCCATT TTCAGAACTT TTCAAAGGAT TCATACAGGC ATGTAGAAAA
    CAGGCCCCAA AGACAGATAT AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC
    TTTGCTGCTC TGGTGTGTCG TACTCCAGGC TTATATGAGA AGCTGCAAAA ATATGTAAAC
    AGTGGAGGCA ACACAGCCCT GAGCGAGGAC TTTGCACAGG TGTATTGCTT GGCAGATGGG
    ATCCGAGTAT GGATGCTGGA GATGAAGCAG AAGTCATTGA TGAACCTGGG GAATGAGGCA
    GAAGAAAAGC ATGGCCCGGA AGCTGCCGAG GCAAACCCTG AGAGCCTGGC AAAAGAGTGT
    ATTCAGAAGA GTCTTCTGTT ACTACGATTT TTGCCCATGG GAGTAAGTTC AAAAGAAAGC
    TGTGACAAGC TGGTGGCTGC AGTAGAAACA GATCACCTGC AGCCCTGCGA CAGGTGCCCA
    AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGG AGTCTCCCAT GGACACAGGC
    ACCCTTGCCG CAAGCAGTGG GGGTCCTAGC TCAGATGTGC CGCTGAAGCT GCTGCCCAGC
    GGTGGCCCCC CTGCCACTGA GGTGTCCTTT GCCACTGCTG AAGAGCCCTC TTCACCTTCC
    GCTCCTGCCC GGCGGCCTCC CTTCACCCGA GGGCGACTTC GACTGCTCTC CTTCCGGTCA
    GTGGAGGAGG CCAGACCCGT GCCCACCGTG AAGGAGAAAT ACCCCGTGCT GAAGGATGTC
    CTGGACTTCA TTAAGGACCA GTCCCTCTCT CATGAGAGTG TCGTAAAGGT TCTTGCCTTG
    AGGAAAGCTC AAGCTCAGAG CATCCTGGAG GTCCTGAGGA TCATTCAGTA CTGCACGGAG
    TCTCTGGGAC GGCCTCATTG CTTCCACCCG CCGTGTATAC TTTTCCTGAT GGAGCTTCTC
    ACCTGCCAGA AAGAGTTTAC CAATTATTTC GCACACATGG AGGGCTGTGG TGCACGTCTG
    CGCAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTTAA AGCAGTTAAA
    GAATTTAGCA GCATGAACGA CAGGTCGCTG CTGCCTGCCT TGTCCTGTGT GCAGACAGCC
    CTGCTCCACC TTCTGGATAT GGGCTGGGAA CCCGGCGATC TCGCTTTCTT TGTGGACATT
    GGGTTACCAC ACCTCCTCAT GAAACTGTCA CAAGAGAATA TAAGCGTCCA CGACAGCGTG
    ATCAGCCAGT GCAGTGAAGA AGATGAGCTC GCGGATGCCA AGCAGAATTC AGAATGGATG
    GATGAGTGTC AGGATGGCAT GTTTGAGGCC TGGTATGAAA AAATAGCCCA GGAAGATCCA
    GAGAAGCAGA GGAAAATGCA CATGTTCATT GCTCGCTACT GCGACCTGCT AAACGTGGAC
    ATCTCCTGTG ATGGCTGTGA TGAGATTGCC CCCTGGCACC GCTACCGGTG TCTGCAGTGC
    AGTGACATGG ATCTCTGCAA AACTTGCTTC CTAGGTGGGG TGAAGCCCGA GGGCCATGGA
    GATGATCATG AGATGGTCAG CATGGAGTTT ACCTGTGACC ACTGCCAGGG TTTGATCATC
    GGCCGGAGGA TGAACTGCAA CGTGTGTGAT GACTTCGATC TTTGCTACGG GTGCTACGCG
    GCAAAGAGAT ACTCTTACGG CCACTTGCCC ACCCACGGCG TCACAGCCCA CCCGATGGTG
    ACCATCCGGA TCAGCGACCG GCAGAGGCTT ATCCAGCCGT ACATCCACAA CTACGCCTGG
    CTGCTCTTTG CTGCCCTGGC TCTCTACAGC GCCCACCTGG CCAGTGCAGA GGACGTGGAT
    GGTGAGAAGC TGAATCCCCA GGACCGTGGC AGTGCCAGCC TGCTGCGGAG CCAGTGCATG
    CAGCTTGTCG GGGACTGTCT GATGAAGGCT CACCAGGGAA AAGGTCTGAA AGCTCTAGCT
    CTCCTTGGTA TATTGCCAGA TGGTGAACCT AGTCCAGAAA GTCGGGCCCC ACCCGTCACT
    GTGCCCACCC ACACGGCAGA GGAGGAGAGA GCTGTCTGGG GCCCTGAGGA CCTGCCACAT
    ACAGGCCTTT CTATGTGCTA CAGTGGAAAG GAAGATGGTC CTAAGGAAGT CAGACCTTTA
    GATAGCAGGC AGAGAAGTAA GGCAGATGAA GAAGGTGCAT CCTTAATGAA TGGTCCTTCA
    TGCCAGACTC CAATCTCAGA CACACCTGCA GATGGTAGCA CCCCTGCAAG GCTTCCAGAT
    GCTGAGAATA CAGTCATGTT GTCTCAGGAG TCCATCGAGG AAAAGGCGGT TACTCCAAGT
    CCTGAGCAGG TGTTTGCTGA GTGCTCCCAG AAGAGGATTT TAGGATTACT AGCAGCCATG
    TTACCTCCCT TAAAGTTGGA CCCCACAGTC CCCGTGATAG ACCTGGAGCA CGTCCTCCCA
    CTCATGTTTC AGGTTGTCAT CTCAAACGTA GGCCACCTGA ATGAGACCTA CCACCTCACG
    CTGGGTCTTC TCGGCCAGCT CATCACCCGC CTCTTACCAG CGGAGGTAGA TGCTGCGGTG
    GTGAAAGTCC TCTCCGCCAA GCATACCTTG TTTGCAACAG GGGACAGTTC TGTGGTGCCA
    GATGGCTGGA AGACCACCCA CCTGCTCTTT AGCCTGGGAG CCGTGTGTCT GGACAGCCGG
    GTGGGCTTGG ACTGGGCATG CTCCATGGCA GAGATCCTGC GGTCCCTCAA CAGTGCCCCA
    CTGTGGCGTG ACGTCATCGC CGCCTTCACA GACCACTGTA TCAGGCAGCT GCCTTTCCAG
    CTGAAGCACA CCAACATCTT CACCCTGCTC GTGCTGGTCG GCTTCCCACA GGTCCTCTGC
    GTGGGGACCC GCTGTGTGTA TGTGGATCAT GCCAATGAGC CACATAATGT CATCATCTTG
    AAGCACTTCA CGGAGAAGAA CAGGGCTGTG ATCGTTGATG TCAAAACCCG GAAAAGGAAA
    ACAGTGAAGG ACTACCAGCT GGTCCAGAAA GGACAGGAAT GCAGCAGCTC TCAGGCCCAG
    CTGAGCCAGT ACTCCCGGCA CTTTGCCTTT ATCGCCAGTC ACCTTCTACA GACCAGTATG
    GACAGCCAAT GTCCTGAGGC AGTAGAAGCA ACATGGGTCC TGTCTCTGGC CCTGAAAGGA
    CTGTATAAAA CACTAAAGGC TCACAGTTTT GAAGAGACCC ACACCACCTT CCTTCAGACG
    GATTTGCTGA AGCTGCTGGT GAAAAAGTGC AGCAAAGGGA CTGGTTTCAG TAAAACGTGG
    CTCCTCCGGG ATCTGGAGAT CCTGTCCATC ATGCTGTACT CCTCCAAGAA GGAGAGCCAT
    ACCTCGGCTG AGCACACAGC CCTGGAGCGA GATGAGAGAG GAGACCAAGA GGAGGAGGTG
    GATGGCACAG TCAGCAGCCC TGGTGAGCCA GACCAAAAGA AGCTAGACCC TCTGGAGAGC
    CTGGACGAGC CTACCAGAAT ATGTTTCTTG ATGGCGCACG ATGCGCTCAA TGCTCCTCTG
    CACATCCTCC GGGCCATCTA CGAGCTGCAG ATGAAAAGGA CGGATTCTTT CTTCCTGGAG
    GTTCAGAAGA GGTTTGATGG GGATGAACTC ACCACAGATG AGAGGATTCG CACCCTGGCT
    CAGAGATGGC AGCCCAGTAG GGGCCTGAGA CTGGAGGAAC AGAGCACCAA AGCCGTGGAT
    ACAGACATGA TCATCTTACC GTGTTTGTCC CGGCCTGCCC GCTGTGACCA GGCCACGGCC
    GAATCCAACC CCGTGACCCA GAAGCTGATC TCCAGCACTG AGAGTGAATT GCAGCATAGC
    TACGCCAAAC AGCGGCGCAG CAAGAGCACC GCCCTCCTGC ACAAGGAGCT GAATGGCAAG
    AGCAAGCGGG CCATCCGGGA GTACCTGTTC CGCGTGAACG AAGCAACAGC TGTCCTGTAT
    GCCCGCCATG TGCTGGCATC CTTGCTCGCC GAGTGGCCGG GGCACGTACC AGTGAGCGAG
    GACACCCTGG ACCTCAGCGG CCCTGCCCAC ATGACCTACA TCTTGGACAT GTTCATGCAG
    CTGGAGGAGA AGCACCAGTG GGAGAAGATC CTGCGGAAGG TGCTTCAGGG CTGCCGAGGG
    AGCATGCTGG GGACCATGGC TCTGGCCGCC TGCCAGTTCA TGGAGGAGCC GGGGATGGAG
    GTGCAAGTGA GAGAGTCAAA GCACCCGTAT AACAACAACA CCAGCTTTGA GGATAAAGTC
    CACATCCCTG GTGCAATCTA CCTCTCGATC AAATTTGACT CGCAGTGTAG CACAGAGGAG
    GGCTGTGACG AGCTAGCCAT GTCCAGCAGT AGCGACTTCC AGCAGGATCG ACACACTTTC
    AGTGGGTCTC AGCAGAAATG GAAGGATTTT GAGCTTCCAG GGGATACTCT ATACTACCGC
    TTCACCTCTG ACATGAGCAA CACGGAGTGG GGTTACAAGT TCACTGTGAC AGCTGGACAC
    CTGGGCCGAT TCCAGACAGG CTTCGAGATT TTGAAGCAGA TGTTGTCAGA AGAAAGAGTT
    GTGCCACACC TCCCACTGGC CAAAATTTGG GAGTGGCTGG TGGGGGTGGC TTGTCGCCAG
    ACTGGTCATC AGCGATTGAA AGCCATCCAC CTGCTTCTGA GGATTGTGCA ATGCTGCACC
    CACAGCGACC TCTGTGACCT TGCGCTGCTA AAGCCCCTGT GGCAGCTCTT CACTCACATG
    GAGTATAGCC TGTTCGAGGA TGTGACCTCA CCCGGCATCC TCCTCCCCCT GCACCGTGCC
    CTCACTGAGC TCTTCTTCGT CACCGAGAAC CGCGCCCAGG AGCTCGGCTT GCTGCAGGAG
    TATCTGCTGG CCTTGACCAC GGACGACCAC CTTCTCCGCT GCGCGGCACA GGCCCTGCAG
    AATGTCGCTG CTATCAGCCT GGCCATCAAC TACCCAAACA AGGCAACCCG CCTCTGGAAC
    GTTGAGTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06092
    Clone ID Related Accession (Same CDS sequence) XM_546554.5 , XM_546554.6
    Accession Version XM_546554.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8892bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546554.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAATG TCACCGAGCA GGAACACAAG CAGTCCCAGC AGCTCACTTT CCAGTCACTA
    TGCACCTATT TTAGTGACAA GGATCCAGGT GGTCTTCTGC TCTTGCCTGA GAAAGGTGAC
    CTGGCTGACA TGAACAGCAG CGAAGTTCTG GCCGTCATGA GCACCCTCCT GTCCGTTGCT
    GCTCGAGAGT GTGAGCTCCT GCTGCTTACC AGGGCCCAGG GACAGCCCGG CTCCGTGCTC
    TTCTCCCTGT TCTGGGCCGT CCAGGGCAGC CTGCTCTCCT GGTGCTACCT GCAGCTGAAG
    AGCACCGACG CGGGAGCCCG GGGTCTCGCC GCCCACCTCA TTGACCAGTA TGTGGGACAG
    TTTGTGGCAA GCACGAGAGT GATTCTGGAA TCCCTTTTGT CACAGTATAG TGGACAAACC
    ATTGTAGAGA AACTGTGTAA TTCAGTGTTT TCGATGGCAG CTCGTCAACT GGTGATCTTC
    CTGCTGGACT TTTGCACTTT AGACATTTCA CACTGTGCAC TCCTGAGAGA GTTCAGTACA
    CTGACAGAAC TGTTAAAGCA GATCTGCGCG GACCCCACAG GAGCGCCAGA CAAGCTCGAT
    GAAGACACTT GGCAGCAGGA GTGTCCTGTG GTATTACACA CCTGGACCAA GGAGTCTGCC
    CACAACTATG AGAACAATTG CCATGAGGTG TCTGTCTTCA TCAGCCCTGG GGCAACCTAC
    TTTGAGGTAG AATTTGATGA GCGCTGTGAG ACTGAGAAGA GGTACGATTA TTTGGAGTTC
    ACTGACGCTA GAGGCGGGAA AACACGCTAT GACACCAAAG TTGGCACTGA GAAGTGGCCC
    AGGAAAGTGA TCTTCAAGGC TGGCCCCCGG CTGCAGTTCC TCTTTCACTC TGACAGCAGT
    AACAATGAGT GGGGTTACAA ATTCACCGTC ACCGCCTATG GCCTGCCCGA TGTGGCCGTG
    TCCTGGGCCC TGGATTTACA GCTGCTCGTT TGCCGGCTGA TGGGACGCCT CGCTGCCCAG
    TGCATGGCGC TCAAGTCTGC GCACCAGTTT GGAGGCGACA TGGCAGTGGC TCCAGCAAAA
    ATGGCGTTGG TCCTGAACTC TCCGCTGTGG AAACCTGTTT TCAGGCATCA GTTGAGTCCA
    GAGTTGGGAT TAGCAGTGAG CTGGCCCACG CACCTGCACC AGGATAGTAA GGAGGTCAAG
    AACATCCCCG ATGATCCCTG CCACCACTTT CTTCTTGAGT TTGCACAGTC AGACCCTGCC
    CAGAACTTCT GTGGGCCATT TTCAGAACTT TTCAAAGGAT TCATACAGGC ATGTAGAAAA
    CAGGCCCCAA AGACAGATAT AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC
    TTTGCTGCTC TGGTGTGTCG TACTCCAGGC TTATATGAGA AGCTGCAAAA ATATGTAAAC
    AGTGGAGGCA ACACAGCCCT GAGCGAGGAC TTTGCACAGG TGTATTGCTT GGCAGATGGG
    ATCCGAGTAT GGATGCTGGA GATGAAGCAG AAGTCATTGA TGAACCTGGG GAATGAGGCA
    GAAGAAAAGC ATGGCCCGGA AGCTGCCGAG GCAAACCCTG AGAGCCTGGC AAAAGAGTGT
    ATTCAGAAGA GTCTTCTGTT ACTACGATTT TTGCCCATGG GAGTAAGTTC AAAAGAAAGC
    TGTGACAAGC TGGTGGCTGC AGTAGAAACA GATCACCTGC AGCCCTGCGA CAGGTGCCCA
    AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGG AGTCTCCCAT GGACACAGGC
    ACCCTTGCCG CAAGCAGTGG GGGTCCTAGC TCAGATGTGC CGCTGAAGCT GCTGCCCAGC
    GGTGGCCCCC CTGCCACTGA GGTGTCCTTT GCCACTGCTG AAGAGCCCTC TTCACCTTCC
    GCTCCTGCCC GGCGGCCTCC CTTCACCCGA GGGCGACTTC GACTGCTCTC CTTCCGGTCA
    GTGGAGGAGG CCAGACCCGT GCCCACCGTG AAGGAGAAAT ACCCCGTGCT GAAGGATGTC
    CTGGACTTCA TTAAGGACCA GTCCCTCTCT CATGAGAGTG TCGTAAAGGT TCTTGCCTTG
    AGGAAAGCTC AAGCTCAGAG CATCCTGGAG GTCCTGAGGA TCATTCAGTA CTGCACGGAG
    TCTCTGGGAC GGCCTCATTG CTTCCACCCG CCGTGTATAC TTTTCCTGAT GGAGCTTCTC
    ACCTGCCAGA AAGAGTTTAC CAATTATTTC GCACACATGG AGGGCTGTGG TGCACGTCTG
    CGCAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTTAA AGCAGTTAAA
    GAATTTAGCA GCATGAACGA CAGGTCGCTG CTGCCTGCCT TGTCCTGTGT GCAGACAGCC
    CTGCTCCACC TTCTGGATAT GGGCTGGGAA CCCGGCGATC TCGCTTTCTT TGTGGACATT
    GGGTTACCAC ACCTCCTCAT GAAACTGTCA CAAGAGAATA TAAGCGTCCA CGACAGCGTG
    ATCAGCCAGT GCAGTGAAGA AGATGAGCTC GCGGATGCCA AGCAGAATTC AGAATGGATG
    GATGAGTGTC AGGATGGCAT GTTTGAGGCC TGGTATGAAA AAATAGCCCA GGAAGATCCA
    GAGAAGCAGA GGAAAATGCA CATGTTCATT GCTCGCTACT GCGACCTGCT AAACGTGGAC
    ATCTCCTGTG ATGGCTGTGA TGAGATTGCC CCCTGGCACC GCTACCGGTG TCTGCAGTGC
    AGTGACATGG ATCTCTGCAA AACTTGCTTC CTAGGTGGGG TGAAGCCCGA GGGCCATGGA
    GATGATCATG AGATGGTCAG CATGGAGTTT ACCTGTGACC ACTGCCAGGG TTTGATCATC
    GGCCGGAGGA TGAACTGCAA CGTGTGTGAT GACTTCGATC TTTGCTACGG GTGCTACGCG
    GCAAAGAGAT ACTCTTACGG CCACTTGCCC ACCCACGGCG TCACAGCCCA CCCGATGGTG
    ACCATCCGGA TCAGCGACCG GCAGAGGCTT ATCCAGCCGT ACATCCACAA CTACGCCTGG
    CTGCTCTTTG CTGCCCTGGC TCTCTACAGC GCCCACCTGG CCAGTGCAGA GGACGTGGAT
    GGTGAGAAGC TGAATCCCCA GGACCGTGGC AGTGCCAGCC TGCTGCGGAG CCAGTGCATG
    CAGCTTGTCG GGGACTGTCT GATGAAGGCT CACCAGGGAA AAGGTCTGAA AGCTCTAGCT
    CTCCTTGGTA TATTGCCAGA TGGTGAACCT AGTCCAGAAA GTCGGGCCCC ACCCGTCACT
    GTGCCCACCC ACACGGCAGA GGAGGAGAGA GCTGTCTGGG GCCCTGAGGA CCTGCCACAT
    ACAGGCCTTT CTATGTGCTA CAGTGGAAAG GAAGATGGTC CTAAGGAAGT CAGACCTTTA
    GATAGCAGGC AGAGAAGTAA GGCAGATGAA GAAGGTGCAT CCTTAATGAA TGGTCCTTCA
    TGCCAGACTC CAATCTCAGA CACACCTGCA GATGGTAGCA CCCCTGCAAG GCTTCCAGAT
    GCTGAGAATA CAGTCATGTT GTCTCAGGAG TCCATCGAGG AAAAGGCGGT TACTCCAAGT
    CCTGAGCAGG TGTTTGCTGA GTGCTCCCAG AAGAGGATTT TAGGATTACT AGCAGCCATG
    TTACCTCCCT TAAAGTTGGA CCCCACAGTC CCCGTGATAG ACCTGGAGCA CGTCCTCCCA
    CTCATGTTTC AGGTTGTCAT CTCAAACGTA GGCCACCTGA ATGAGACCTA CCACCTCACG
    CTGGGTCTTC TCGGCCAGCT CATCACCCGC CTCTTACCAG CGGAGGTAGA TGCTGCGGTG
    GTGAAAGTCC TCTCCGCCAA GCATACCTTG TTTGCAACAG GGGACAGTTC TGTGGTGCCA
    GATGGCTGGA AGACCACCCA CCTGCTCTTT AGCCTGGGAG CCGTGTGTCT GGACAGCCGG
    GTGGGCTTGG ACTGGGCATG CTCCATGGCA GAGATCCTGC GGTCCCTCAA CAGTGCCCCA
    CTGTGGCGTG ACGTCATCGC CGCCTTCACA GACCACTGTA TCAGGCAGCT GCCTTTCCAG
    CTGAAGCACA CCAACATCTT CACCCTGCTC GTGCTGGTCG GCTTCCCACA GGTCCTCTGC
    GTGGGGACCC GCTGTGTGTA TGTGGATCAT GCCAATGAGC CACATAATGT CATCATCTTG
    AAGCACTTCA CGGAGAAGAA CAGGGCTGTG ATCGTTGATG TCAAAACCCG GAAAAGGAAA
    ACAGTGAAGG ACTACCAGCT GGTCCAGAAA GGACAGGAAT GCAGCAGCTC TCAGGCCCAG
    CTGAGCCAGT ACTCCCGGCA CTTTGCCTTT ATCGCCAGTC ACCTTCTACA GACCAGTATG
    GACAGCCAAT GTCCTGAGGC AGTAGAAGCA ACATGGGTCC TGTCTCTGGC CCTGAAAGGA
    CTGTATAAAA CACTAAAGGC TCACAGTTTT GAAGAGACCC ACACCACCTT CCTTCAGACG
    GATTTGCTGA AGCTGCTGGT GAAAAAGTGC AGCAAAGGGA CTGGTTTCAG TAAAACGTGG
    CTCCTCCGGG ATCTGGAGAT CCTGTCCATC ATGCTGTACT CCTCCAAGAA GGAGAGCCAT
    ACCTCGGCTG AGCACACAGC CCTGGAGCGA GATGAGAGAG GAGACCAAGA GGAGGAGGTG
    GATGGCACAG TCAGCAGCCC TGGTGAGCCA GACCAAAAGA AGCTAGACCC TCTGGAGAGC
    CTGGACGAGC CTACCAGAAT ATGTTTCTTG ATGGCGCACG ATGCGCTCAA TGCTCCTCTG
    CACATCCTCC GGGCCATCTA CGAGCTGCAG ATGAAAAGGA CGGATTCTTT CTTCCTGGAG
    GTTCAGAAGA GGTTTGATGG GGATGAACTC ACCACAGATG AGAGGATTCG CACCCTGGCT
    CAGAGATGGC AGCCCAGTAG GGGCCTGAGA CTGGAGGAAC AGAGCACCAA AGCCGTGGAT
    ACAGACATGA TCATCTTACC GTGTTTGTCC CGGCCTGCCC GCTGTGACCA GGCCACGGCC
    GAATCCAACC CCGTGACCCA GAAGCTGATC TCCAGCACTG AGAGTGAATT GCAGCATAGC
    TACGCCAAAC AGCGGCGCAG CAAGAGCACC GCCCTCCTGC ACAAGGAGCT GAATGGCAAG
    AGCAAGCGGG CCATCCGGGA GTACCTGTTC CGCGTGAACG AAGCAACAGC TGTCCTGTAT
    GCCCGCCATG TGCTGGCATC CTTGCTCGCC GAGTGGCCGG GGCACGTACC AGTGAGCGAG
    GACACCCTGG ACCTCAGCGG CCCTGCCCAC ATGACCTACA TCTTGGACAT GTTCATGCAG
    CTGGAGGAGA AGCACCAGTG GGAGAAGATC CTGCGGAAGG TGCTTCAGGG CTGCCGAGGG
    AGCATGCTGG GGACCATGGC TCTGGCCGCC TGCCAGTTCA TGGAGGAGCC GGGGATGGAG
    GTGCAAGTGA GAGAGTCAAA GCACCCGTAT AACAACAACA CCAGCTTTGA GGATAAAGTC
    CACATCCCTG GTGCAATCTA CCTCTCGATC AAATTTGACT CGCAGTGTAG CACAGAGGAG
    GGCTGTGACG AGCTAGCCAT GTCCAGCAGT AGCGACTTCC AGCAGGATCG ACACACTTTC
    AGTGGGTCTC AGCAGAAATG GAAGGATTTT GAGCTTCCAG GGGATACTCT ATACTACCGC
    TTCACCTCTG ACATGAGCAA CACGGAGTGG GGTTACAAGT TCACTGTGAC AGCTGGACAC
    CTGGGCCGAT TCCAGACAGG CTTCGAGATT TTGAAGCAGA TGTTGTCAGA AGAAAGAGTT
    GTGCCACACC TCCCACTGGC CAAAATTTGG GAGTGGCTGG TGGGGGTGGC TTGTCGCCAG
    ACTGGTCATC AGCGATTGAA AGCCATCCAC CTGCTTCTGA GGATTGTGCA ATGCTGCACC
    CACAGCGACC TCTGTGACCT TGCGCTGCTA AAGCCCCTGT GGCAGCTCTT CACTCACATG
    GAGTATAGCC TGTTCGAGGA TGTGACCTCA CCCGGCATCC TCCTCCCCCT GCACCGTGCC
    CTCACTGAGC TCTTCTTCGT CACCGAGAAC CGCGCCCAGG AGCTCGGCTT GCTGCAGGAG
    TATCTGCTGG CCTTGACCAC GGACGACCAC CTTCTCCGCT GCGCGGCACA GGCCCTGCAG
    AATGTCGCTG CTATCAGCCT GGCCATCAAC TACCCAAACA AGGCAACCCG CCTCTGGAAC
    GTTGAGTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_546554.3
    CDS1..8892
    Translation

    Target ORF information:

    RefSeq Version XM_546554.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_546554.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAATG TCACCGAGCA GGAACACAAG CAGTCCCAGC AGCTCACTTT CCAGTCACTA
    TGCACCTATT TTAGTGACAA GGATCCAGGT GGTCTTCTGC TCTTGCCTGA GAAAGGTGAC
    CTGGCTGACA TGAACAGCAG CGAAGTTCTG GCCGTCATGA GCACCCTCCT GTCCGTTGCT
    GCTCGAGAGT GTGAGCTCCT GCTGCTTACC AGGGCCCAGG GACAGCCCGG CTCCGTGCTC
    TTCTCCCTGT TCTGGGCCGT CCAGGGCAGC CTGCTCTCCT GGTGCTACCT GCAGCTGAAG
    AGCACCGACG CGGGAGCCCG GGGTCTCGCC GCCCACCTCA TTGACCAGTA TGTGGGACAG
    TTTGTGGCAA GCACGAGAGT GATTCTGGAA TCCCTTTTGT CACAGTATAG TGGACAAACC
    ATTGTAGAGA AACTGTGTAA TTCAGTGTTT TCGATGGCAG CTCGTCAACT GGTGATCTTC
    CTGCTGGACT TTTGCACTTT AGACATTTCA CACTGTGCAC TCCTGAGAGA GTTCAGTACA
    CTGACAGAAC TGTTAAAGCA GATCTGCGCG GACCCCACAG GAGCGCCAGA CAAGCTCGAT
    GAAGACACTT GGCAGCAGGA GTGTCCTGTG GTATTACACA CCTGGACCAA GGAGTCTGCC
    CACAACTATG AGAACAATTG CCATGAGGTG TCTGTCTTCA TCAGCCCTGG GGCAACCTAC
    TTTGAGGTAG AATTTGATGA GCGCTGTGAG ACTGAGAAGA GGTACGATTA TTTGGAGTTC
    ACTGACGCTA GAGGCGGGAA AACACGCTAT GACACCAAAG TTGGCACTGA GAAGTGGCCC
    AGGAAAGTGA TCTTCAAGGC TGGCCCCCGG CTGCAGTTCC TCTTTCACTC TGACAGCAGT
    AACAATGAGT GGGGTTACAA ATTCACCGTC ACCGCCTATG GCCTGCCCGA TGTGGCCGTG
    TCCTGGGCCC TGGATTTACA GCTGCTCGTT TGCCGGCTGA TGGGACGCCT CGCTGCCCAG
    TGCATGGCGC TCAAGTCTGC GCACCAGTTT GGAGGCGACA TGGCAGTGGC TCCAGCAAAA
    ATGGCGTTGG TCCTGAACTC TCCGCTGTGG AAACCTGTTT TCAGGCATCA GTTGAGTCCA
    GAGTTGGGAT TAGCAGTGAG CTGGCCCACG CACCTGCACC AGGATAGTAA GGAGGTCAAG
    AACATCCCCG ATGATCCCTG CCACCACTTT CTTCTTGAGT TTGCACAGTC AGACCCTGCC
    CAGAACTTCT GTGGGCCATT TTCAGAACTT TTCAAAGGAT TCATACAGGC ATGTAGAAAA
    CAGGCCCCAA AGACAGATAT AGTTGCTGGT TCCACTATTG ATCAAGCTGT GAACGCCACC
    TTTGCTGCTC TGGTGTGTCG TACTCCAGGC TTATATGAGA AGCTGCAAAA ATATGTAAAC
    AGTGGAGGCA ACACAGCCCT GAGCGAGGAC TTTGCACAGG TGTATTGCTT GGCAGATGGG
    ATCCGAGTAT GGATGCTGGA GATGAAGCAG AAGTCATTGA TGAACCTGGG GAATGAGGCA
    GAAGAAAAGC ATGGCCCGGA AGCTGCCGAG GCAAACCCTG AGAGCCTGGC AAAAGAGTGT
    ATTCAGAAGA GTCTTCTGTT ACTACGATTT TTGCCCATGG GAGTAAGTTC AAAAGAAAGC
    TGTGACAAGC TGGTGGCTGC AGTAGAAACA GATCACCTGC AGCCCTGCGA CAGGTGCCCA
    AGGACGAGCT CTGTGGTGGA GGAGCATTTC CAGGCCTCGG AGTCTCCCAT GGACACAGGC
    ACCCTTGCCG CAAGCAGTGG GGGTCCTAGC TCAGATGTGC CGCTGAAGCT GCTGCCCAGC
    GGTGGCCCCC CTGCCACTGA GGTGTCCTTT GCCACTGCTG AAGAGCCCTC TTCACCTTCC
    GCTCCTGCCC GGCGGCCTCC CTTCACCCGA GGGCGACTTC GACTGCTCTC CTTCCGGTCA
    GTGGAGGAGG CCAGACCCGT GCCCACCGTG AAGGAGAAAT ACCCCGTGCT GAAGGATGTC
    CTGGACTTCA TTAAGGACCA GTCCCTCTCT CATGAGAGTG TCGTAAAGGT TCTTGCCTTG
    AGGAAAGCTC AAGCTCAGAG CATCCTGGAG GTCCTGAGGA TCATTCAGTA CTGCACGGAG
    TCTCTGGGAC GGCCTCATTG CTTCCACCCG CCGTGTATAC TTTTCCTGAT GGAGCTTCTC
    ACCTGCCAGA AAGAGTTTAC CAATTATTTC GCACACATGG AGGGCTGTGG TGCACGTCTG
    CGCAAAGAGA TTCGAGACAC TTACTACCAG TTTGTTCTGT TTTTGGTTAA AGCAGTTAAA
    GAATTTAGCA GCATGAACGA CAGGTCGCTG CTGCCTGCCT TGTCCTGTGT GCAGACAGCC
    CTGCTCCACC TTCTGGATAT GGGCTGGGAA CCCGGCGATC TCGCTTTCTT TGTGGACATT
    GGGTTACCAC ACCTCCTCAT GAAACTGTCA CAAGAGAATA TAAGCGTCCA CGACAGCGTG
    ATCAGCCAGT GCAGTGAAGA AGATGAGCTC GCGGATGCCA AGCAGAATTC AGAATGGATG
    GATGAGTGTC AGGATGGCAT GTTTGAGGCC TGGTATGAAA AAATAGCCCA GGAAGATCCA
    GAGAAGCAGA GGAAAATGCA CATGTTCATT GCTCGCTACT GCGACCTGCT AAACGTGGAC
    ATCTCCTGTG ATGGCTGTGA TGAGATTGCC CCCTGGCACC GCTACCGGTG TCTGCAGTGC
    AGTGACATGG ATCTCTGCAA AACTTGCTTC CTAGGTGGGG TGAAGCCCGA GGGCCATGGA
    GATGATCATG AGATGGTCAG CATGGAGTTT ACCTGTGACC ACTGCCAGGG TTTGATCATC
    GGCCGGAGGA TGAACTGCAA CGTGTGTGAT GACTTCGATC TTTGCTACGG GTGCTACGCG
    GCAAAGAGAT ACTCTTACGG CCACTTGCCC ACCCACGGCG TCACAGCCCA CCCGATGGTG
    ACCATCCGGA TCAGCGACCG GCAGAGGCTT ATCCAGCCGT ACATCCACAA CTACGCCTGG
    CTGCTCTTTG CTGCCCTGGC TCTCTACAGC GCCCACCTGG CCAGTGCAGA GGACGTGGAT
    GGTGAGAAGC TGAATCCCCA GGACCGTGGC AGTGCCAGCC TGCTGCGGAG CCAGTGCATG
    CAGCTTGTCG GGGACTGTCT GATGAAGGCT CACCAGGGAA AAGGTCTGAA AGCTCTAGCT
    CTCCTTGGTA TATTGCCAGA TGGTGAACCT AGTCCAGAAA GTCGGGCCCC ACCCGTCACT
    GTGCCCACCC ACACGGCAGA GGAGGAGAGA GCTGTCTGGG GCCCTGAGGA CCTGCCACAT
    ACAGGCCTTT CTATGTGCTA CAGTGGAAAG GAAGATGGTC CTAAGGAAGT CAGACCTTTA
    GATAGCAGGC AGAGAAGTAA GGCAGATGAA GAAGGTGCAT CCTTAATGAA TGGTCCTTCA
    TGCCAGACTC CAATCTCAGA CACACCTGCA GATGGTAGCA CCCCTGCAAG GCTTCCAGAT
    GCTGAGAATA CAGTCATGTT GTCTCAGGAG TCCATCGAGG AAAAGGCGGT TACTCCAAGT
    CCTGAGCAGG TGTTTGCTGA GTGCTCCCAG AAGAGGATTT TAGGATTACT AGCAGCCATG
    TTACCTCCCT TAAAGTTGGA CCCCACAGTC CCCGTGATAG ACCTGGAGCA CGTCCTCCCA
    CTCATGTTTC AGGTTGTCAT CTCAAACGTA GGCCACCTGA ATGAGACCTA CCACCTCACG
    CTGGGTCTTC TCGGCCAGCT CATCACCCGC CTCTTACCAG CGGAGGTAGA TGCTGCGGTG
    GTGAAAGTCC TCTCCGCCAA GCATACCTTG TTTGCAACAG GGGACAGTTC TGTGGTGCCA
    GATGGCTGGA AGACCACCCA CCTGCTCTTT AGCCTGGGAG CCGTGTGTCT GGACAGCCGG
    GTGGGCTTGG ACTGGGCATG CTCCATGGCA GAGATCCTGC GGTCCCTCAA CAGTGCCCCA
    CTGTGGCGTG ACGTCATCGC CGCCTTCACA GACCACTGTA TCAGGCAGCT GCCTTTCCAG
    CTGAAGCACA CCAACATCTT CACCCTGCTC GTGCTGGTCG GCTTCCCACA GGTCCTCTGC
    GTGGGGACCC GCTGTGTGTA TGTGGATCAT GCCAATGAGC CACATAATGT CATCATCTTG
    AAGCACTTCA CGGAGAAGAA CAGGGCTGTG ATCGTTGATG TCAAAACCCG GAAAAGGAAA
    ACAGTGAAGG ACTACCAGCT GGTCCAGAAA GGACAGGAAT GCAGCAGCTC TCAGGCCCAG
    CTGAGCCAGT ACTCCCGGCA CTTTGCCTTT ATCGCCAGTC ACCTTCTACA GACCAGTATG
    GACAGCCAAT GTCCTGAGGC AGTAGAAGCA ACATGGGTCC TGTCTCTGGC CCTGAAAGGA
    CTGTATAAAA CACTAAAGGC TCACAGTTTT GAAGAGACCC ACACCACCTT CCTTCAGACG
    GATTTGCTGA AGCTGCTGGT GAAAAAGTGC AGCAAAGGGA CTGGTTTCAG TAAAACGTGG
    CTCCTCCGGG ATCTGGAGAT CCTGTCCATC ATGCTGTACT CCTCCAAGAA GGAGAGCCAT
    ACCTCGGCTG AGCACACAGC CCTGGAGCGA GATGAGAGAG GAGACCAAGA GGAGGAGGTG
    GATGGCACAG TCAGCAGCCC TGGTGAGCCA GACCAAAAGA AGCTAGACCC TCTGGAGAGC
    CTGGACGAGC CTACCAGAAT ATGTTTCTTG ATGGCGCACG ATGCGCTCAA TGCTCCTCTG
    CACATCCTCC GGGCCATCTA CGAGCTGCAG ATGAAAAGGA CGGATTCTTT CTTCCTGGAG
    GTTCAGAAGA GGTTTGATGG GGATGAACTC ACCACAGATG AGAGGATTCG CACCCTGGCT
    CAGAGATGGC AGCCCAGTAG GGGCCTGAGA CTGGAGGAAC AGAGCACCAA AGCCGTGGAT
    ACAGACATGA TCATCTTACC GTGTTTGTCC CGGCCTGCCC GCTGTGACCA GGCCACGGCC
    GAATCCAACC CCGTGACCCA GAAGCTGATC TCCAGCACTG AGAGTGAATT GCAGCATAGC
    TACGCCAAAC AGCGGCGCAG CAAGAGCACC GCCCTCCTGC ACAAGGAGCT GAATGGCAAG
    AGCAAGCGGG CCATCCGGGA GTACCTGTTC CGCGTGAACG AAGCAACAGC TGTCCTGTAT
    GCCCGCCATG TGCTGGCATC CTTGCTCGCC GAGTGGCCGG GGCACGTACC AGTGAGCGAG
    GACACCCTGG ACCTCAGCGG CCCTGCCCAC ATGACCTACA TCTTGGACAT GTTCATGCAG
    CTGGAGGAGA AGCACCAGTG GGAGAAGATC CTGCGGAAGG TGCTTCAGGG CTGCCGAGGG
    AGCATGCTGG GGACCATGGC TCTGGCCGCC TGCCAGTTCA TGGAGGAGCC GGGGATGGAG
    GTGCAAGTGA GAGAGTCAAA GCACCCGTAT AACAACAACA CCAGCTTTGA GGATAAAGTC
    CACATCCCTG GTGCAATCTA CCTCTCGATC AAATTTGACT CGCAGTGTAG CACAGAGGAG
    GGCTGTGACG AGCTAGCCAT GTCCAGCAGT AGCGACTTCC AGCAGGATCG ACACACTTTC
    AGTGGGTCTC AGCAGAAATG GAAGGATTTT GAGCTTCCAG GGGATACTCT ATACTACCGC
    TTCACCTCTG ACATGAGCAA CACGGAGTGG GGTTACAAGT TCACTGTGAC AGCTGGACAC
    CTGGGCCGAT TCCAGACAGG CTTCGAGATT TTGAAGCAGA TGTTGTCAGA AGAAAGAGTT
    GTGCCACACC TCCCACTGGC CAAAATTTGG GAGTGGCTGG TGGGGGTGGC TTGTCGCCAG
    ACTGGTCATC AGCGATTGAA AGCCATCCAC CTGCTTCTGA GGATTGTGCA ATGCTGCACC
    CACAGCGACC TCTGTGACCT TGCGCTGCTA AAGCCCCTGT GGCAGCTCTT CACTCACATG
    GAGTATAGCC TGTTCGAGGA TGTGACCTCA CCCGGCATCC TCCTCCCCCT GCACCGTGCC
    CTCACTGAGC TCTTCTTCGT CACCGAGAAC CGCGCCCAGG AGCTCGGCTT GCTGCAGGAG
    TATCTGCTGG CCTTGACCAC GGACGACCAC CTTCTCCGCT GCGCGGCACA GGCCCTGCAG
    AATGTCGCTG CTATCAGCCT GGCCATCAAC TACCCAAACA AGGCAACCCG CCTCTGGAAC
    GTTGAGTGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06093
    Clone ID Related Accession (Same CDS sequence) XM_005619866.2 , XM_005619866.3
    Accession Version XM_005619866.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAAGA ATGTCACCGA GCAGGAACAC AAGCAGTCCC AGCAGCTCAC TTTCCAGTCA
    CTATGCACCT ATTTTAGTGA CAAGGATCCA GGTGGTCTTC TGCTCTTGCC TGAGAAAGGT
    GACCTGGCTG ACATGAACAG CAGCGAAGTT CTGGCCGTCA TGAGCACCCT CCTGTCCGTT
    GCTGCTCGAG AGTGTGAGCT CCTGCTGCTT ACCAGGGCCC AGGGACAGCC CGGCTCCGTG
    CTCTTCTCCC TGTTCTGGGC CGTCCAGGGC AGCCTGCTCT CCTGGTGCTA CCTGCAGCTG
    AAGAGCACCG ACGCGGGAGC CCGGGGTCTC GCCGCCCACC TCATTGACCA GTATGTGGGA
    CAGTTTGTGG CAAGCACGAG AGTGATTCTG GAATCCCTTT TGTCACAGTA TAGTGGACAA
    ACCATTGTAG AGAAACTGTG TAATTCAGTG TTTTCGATGG CAGCTCGTCA ACTGGTGATC
    TTCCTGCTGG ACTTTTGCAC TTTAGACATT TCACACTGTG CACTCCTGAG AGAGTTCAGT
    ACACTGACAG AACTGTTAAA GCAGATCTGC GCGGACCCCA CAGGAGCGCC AGACAAGCTC
    GATGAAGACA CTTGGCAGCA GGAGTGTCCT GTGGTATTAC ACACCTGGAC CAAGGAGTCT
    GCCCACAACT ATGAGAACAA TTGCCATGAG GTGTCTGTCT TCATCAGCCC TGGGGCAACC
    TACTTTGAGG TAGAATTTGA TGAGCGCTGT GAGACTGAGA AGAGGTACGA TTATTTGGAG
    TTCACTGACG CTAGAGGCGG GAAAACACGC TATGACACCA AAGTTGGCAC TGAGAAGTGG
    CCCAGGAAAG TGATCTTCAA GGCTGGCCCC CGGCTGCAGT TCCTCTTTCA CTCTGACAGC
    AGTAACAATG AGTGGGGTTA CAAATTCACC GTCACCGCCT ATGGCCTGCC CGATGTGGCC
    GTGTCCTGGG CCCTGGATTT ACAGCTGCTC GTTTGCCGGC TGATGGGACG CCTCGCTGCC
    CAGTGCATGG CGCTCAAGTC TGCGCACCAG TTTGGAGGCG ACATGGCAGT GGCTCCAGCA
    AAAATGGCGT TGGTCCTGAA CTCTCCGCTG TGGAAACCTG TTTTCAGGCA TCAGTTGAGT
    CCAGAGTTGG GATTAGCAGT GAGCTGGCCC ACGCACCTGC ACCAGGATAG TAAGGAGGTC
    AAGAACATCC CCGATGATCC CTGCCACCAC TTTCTTCTTG AGTTTGCACA GTCAGACCCT
    GCCCAGAACT TCTGTGGGCC ATTTTCAGAA CTTTTCAAAG GATTCATACA GGCATGTAGA
    AAACAGGCCC CAAAGACAGA TATAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC
    ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GGCTTATATG AGAAGCTGCA AAAATATGTA
    AACAGTGGAG GCAACACAGC CCTGAGCGAG GACTTTGCAC AGGTGTATTG CTTGGCAGAT
    GGGATCCGAG TATGGATGCT GGAGATGAAG CAGAAGTCAT TGATGAACCT GGGGAATGAG
    GCAGAAGAAA AGCATGGCCC GGAAGCTGCC GAGGCAAACC CTGAGAGCCT GGCAAAAGAG
    TGTATTCAGA AGAGTCTTCT GTTACTACGA TTTTTGCCCA TGGGAGTAAG TTCAAAAGAA
    AGCTGTGACA AGCTGGTGGC TGCAGTAGAA ACAGATCACC TGCAGCCCTG CGACAGGTGC
    CCAAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGGAGTCTCC CATGGACACA
    GGCACCCTTG CCGCAAGCAG TGGGGGTCCT AGCTCAGATG TGCCGCTGAA GCTGCTGCCC
    AGCGGTGGCC CCCCTGCCAC TGAGGTGTCC TTTGCCACTG CTGAAGAGCC CTCTTCACCT
    TCCGCTCCTG CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TTCGACTGCT CTCCTTCCGG
    TCAGTGGAGG AGGCCAGACC CGTGCCCACC GTGAAGGAGA AATACCCCGT GCTGAAGGAT
    GTCCTGGACT TCATTAAGGA CCAGTCCCTC TCTCATGAGA GTGTCGTAAA GGTTCTTGCC
    TTGAGGAAAG CTCAAGCTCA GAGCATCCTG GAGGTCCTGA GGATCATTCA GTACTGCACG
    GAGTCTCTGG GACGGCCTCA TTGCTTCCAC CCGCCGTGTA TACTTTTCCT GATGGAGCTT
    CTCACCTGCC AGAAAGAGTT TACCAATTAT TTCGCACACA TGGAGGGCTG TGGTGCACGT
    CTGCGCAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT TAAAGCAGTT
    AAAGAATTTA GCAGCATGAA CGACAGGTCG CTGCTGCCTG CCTTGTCCTG TGTGCAGACA
    GCCCTGCTCC ACCTTCTGGA TATGGGCTGG GAACCCGGCG ATCTCGCTTT CTTTGTGGAC
    ATTGGGTTAC CACACCTCCT CATGAAACTG TCACAAGAGA ATATAAGCGT CCACGACAGC
    GTGATCAGCC AGTGCAGTGA AGAAGATGAG CTCGCGGATG CCAAGCAGAA TTCAGAATGG
    ATGGATGAGT GTCAGGATGG CATGTTTGAG GCCTGGTATG AAAAAATAGC CCAGGAAGAT
    CCAGAGAAGC AGAGGAAAAT GCACATGTTC ATTGCTCGCT ACTGCGACCT GCTAAACGTG
    GACATCTCCT GTGATGGCTG TGATGAGATT GCCCCCTGGC ACCGCTACCG GTGTCTGCAG
    TGCAGTGACA TGGATCTCTG CAAAACTTGC TTCCTAGGTG GGGTGAAGCC CGAGGGCCAT
    GGAGATGATC ATGAGATGGT CAGCATGGAG TTTACCTGTG ACCACTGCCA GGGTTTGATC
    ATCGGCCGGA GGATGAACTG CAACGTGTGT GATGACTTCG ATCTTTGCTA CGGGTGCTAC
    GCGGCAAAGA GATACTCTTA CGGCCACTTG CCCACCCACG GCGTCACAGC CCACCCGATG
    GTGACCATCC GGATCAGCGA CCGGCAGAGG CTTATCCAGC CGTACATCCA CAACTACGCC
    TGGCTGCTCT TTGCTGCCCT GGCTCTCTAC AGCGCCCACC TGGCCAGTGC AGAGGACGTG
    GATGGTGAGA AGCTGAATCC CCAGGACCGT GGCAGTGCCA GCCTGCTGCG GAGCCAGTGC
    ATGCAGCTTG TCGGGGACTG TCTGATGAAG GCTCACCAGG GAAAAGGTCT GAAAGCTCTA
    GCTCTCCTTG GTATATTGCC AGATGGTGAA CCTAGTCCAG AAAGTCGGGC CCCACCCGTC
    ACTGTGCCCA CCCACACGGC AGAGGAGGAG AGAGCTGTCT GGGGCCCTGA GGACCTGCCA
    CATACAGGCC TTTCTATGTG CTACAGTGGA AAGGAAGATG GTCCTAAGGA AGTCAGACCT
    TTAGATAGCA GGCAGAGAAG TAAGGCAGAT GAAGAAGGTG CATCCTTAAT GAATGGTCCT
    TCATGCCAGA CTCCAATCTC AGACACACCT GCAGATGGTA GCACCCCTGC AAGGCTTCCA
    GATGCTGAGA ATACAGTCAT GTTGTCTCAG GAGTCCATCG AGGAAAAGGC GGTTACTCCA
    AGTCCTGAGC AGGTGTTTGC TGAGTGCTCC CAGAAGAGGA TTTTAGGATT ACTAGCAGCC
    ATGTTACCTC CCTTAAAGTT GGACCCCACA GTCCCCGTGA TAGACCTGGA GCACGTCCTC
    CCACTCATGT TTCAGGTTGT CATCTCAAAC GTAGGCCACC TGAATGAGAC CTACCACCTC
    ACGCTGGGTC TTCTCGGCCA GCTCATCACC CGCCTCTTAC CAGCGGAGGT AGATGCTGCG
    GTGGTGAAAG TCCTCTCCGC CAAGCATACC TTGTTTGCAA CAGGGGACAG TTCTGTGGTG
    CCAGATGGCT GGAAGACCAC CCACCTGCTC TTTAGCCTGG GAGCCGTGTG TCTGGACAGC
    CGGGTGGGCT TGGACTGGGC ATGCTCCATG GCAGAGATCC TGCGGTCCCT CAACAGTGCC
    CCACTGTGGC GTGACGTCAT CGCCGCCTTC ACAGACCACT GTATCAGGCA GCTGCCTTTC
    CAGCTGAAGC ACACCAACAT CTTCACCCTG CTCGTGCTGG TCGGCTTCCC ACAGGTCCTC
    TGCGTGGGGA CCCGCTGTGT GTATGTGGAT CATGCCAATG AGCCACATAA TGTCATCATC
    TTGAAGCACT TCACGGAGAA GAACAGGGCT GTGATCGTTG ATGTCAAAAC CCGGAAAAGG
    AAAACAGTGA AGGACTACCA GCTGGTCCAG AAAGGACAGG AATGCAGCAG CTCTCAGGCC
    CAGCTGAGCC AGTACTCCCG GCACTTTGCC TTTATCGCCA GTCACCTTCT ACAGACCAGT
    ATGGACAGCC AATGTCCTGA GGCAGTAGAA GCAACATGGG TCCTGTCTCT GGCCCTGAAA
    GGACTGTATA AAACACTAAA GGCTCACAGT TTTGAAGAGA CCCACACCAC CTTCCTTCAG
    ACGGATTTGC TGAAGCTGCT GGTGAAAAAG TGCAGCAAAG GGACTGGTTT CAGTAAAACG
    TGGCTCCTCC GGGATCTGGA GATCCTGTCC ATCATGCTGT ACTCCTCCAA GAAGGAGAGC
    CATACCTCGG CTGAGCACAC AGCCCTGGAG CGAGATGAGA GAGGAGACCA AGAGGAGGAG
    GTGGATGGCA CAGTCAGCAG CCCTGGTGAG CCAGACCAAA AGAAGCTAGA CCCTCTGGAG
    AGCCTGGACG AGCCTACCAG AATATGTTTC TTGATGGCGC ACGATGCGCT CAATGCTCCT
    CTGCACATCC TCCGGGCCAT CTACGAGCTG CAGATGAAAA GGACGGATTC TTTCTTCCTG
    GAGGTTCAGA AGAGGTTTGA TGGGGATGAA CTCACCACAG ATGAGAGGAT TCGCACCCTG
    GCTCAGAGAT GGCAGCCCAG TAGGGGCCTG AGACTGGAGG AACAGAGCAC CAAAGCCGTG
    GATACAGACA TGATCATCTT ACCGTGTTTG TCCCGGCCTG CCCGCTGTGA CCAGGCCACG
    GCCGAATCCA ACCCCGTGAC CCAGAAGCTG ATCTCCAGCA CTGAGAGTGA ATTGCAGCAT
    AGCTACGCCA AACAGCGGCG CAGCAAGAGC ACCGCCCTCC TGCACAAGGA GCTGAATGGC
    AAGAGCAAGC GGGCCATCCG GGAGTACCTG TTCCGCGTGA ACGAAGCAAC AGCTGTCCTG
    TATGCCCGCC ATGTGCTGGC ATCCTTGCTC GCCGAGTGGC CGGGGCACGT ACCAGTGAGC
    GAGGACACCC TGGACCTCAG CGGCCCTGCC CACATGACCT ACATCTTGGA CATGTTCATG
    CAGCTGGAGG AGAAGCACCA GTGGGAGAAG ATCCTGCGGA AGGTGCTTCA GGGCTGCCGA
    GGGAGCATGC TGGGGACCAT GGCTCTGGCC GCCTGCCAGT TCATGGAGGA GCCGGGGATG
    GAGGTGCAAG TGAGAGAGTC AAAGCACCCG TATAACAACA ACACCAGCTT TGAGGATAAA
    GTCCACATCC CTGGTGCAAT CTACCTCTCG ATCAAATTTG ACTCGCAGTG TAGCACAGAG
    GAGGGCTGTG ACGAGCTAGC CATGTCCAGC AGTAGCGACT TCCAGCAGGA TCGACACACT
    TTCAGTGGGT CTCAGCAGAA ATGGAAGGAT TTTGAGCTTC CAGGGGATAC TCTATACTAC
    CGCTTCACCT CTGACATGAG CAACACGGAG TGGGGTTACA AGTTCACTGT GACAGCTGGA
    CACCTGGGCC GATTCCAGAC AGGCTTCGAG ATTTTGAAGC AGATGTTGTC AGAAGAAAGA
    GTTGTGCCAC ACCTCCCACT GGCCAAAATT TGGGAGTGGC TGGTGGGGGT GGCTTGTCGC
    CAGACTGGTC ATCAGCGATT GAAAGCCATC CACCTGCTTC TGAGGATTGT GCAATGCTGC
    ACCCACAGCG ACCTCTGTGA CCTTGCGCTG CTAAAGCCCC TGTGGCAGCT CTTCACTCAC
    ATGGAGTATA GCCTGTTCGA GGATGTGACC TCACCCGGCA TCCTCCTCCC CCTGCACCGT
    GCCCTCACTG AGCTCTTCTT CGTCACCGAG AACCGCGCCC AGGAGCTCGG CTTGCTGCAG
    GAGTATCTGC TGGCCTTGAC CACGGACGAC CACCTTCTCC GCTGCGCGGC ACAGGCCCTG
    CAGAATGTCG CTGCTATCAG CCTGGCCATC AACTACCCAA ACAAGGCAAC CCGCCTCTGG
    AACGTTGAGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619923.1
    CDS173..9067
    Translation

    Target ORF information:

    RefSeq Version XM_005619866.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris zinc finger, ZZ-type with EF-hand domain 1 (ZZEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619866.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAAGA ATGTCACCGA GCAGGAACAC AAGCAGTCCC AGCAGCTCAC TTTCCAGTCA
    CTATGCACCT ATTTTAGTGA CAAGGATCCA GGTGGTCTTC TGCTCTTGCC TGAGAAAGGT
    GACCTGGCTG ACATGAACAG CAGCGAAGTT CTGGCCGTCA TGAGCACCCT CCTGTCCGTT
    GCTGCTCGAG AGTGTGAGCT CCTGCTGCTT ACCAGGGCCC AGGGACAGCC CGGCTCCGTG
    CTCTTCTCCC TGTTCTGGGC CGTCCAGGGC AGCCTGCTCT CCTGGTGCTA CCTGCAGCTG
    AAGAGCACCG ACGCGGGAGC CCGGGGTCTC GCCGCCCACC TCATTGACCA GTATGTGGGA
    CAGTTTGTGG CAAGCACGAG AGTGATTCTG GAATCCCTTT TGTCACAGTA TAGTGGACAA
    ACCATTGTAG AGAAACTGTG TAATTCAGTG TTTTCGATGG CAGCTCGTCA ACTGGTGATC
    TTCCTGCTGG ACTTTTGCAC TTTAGACATT TCACACTGTG CACTCCTGAG AGAGTTCAGT
    ACACTGACAG AACTGTTAAA GCAGATCTGC GCGGACCCCA CAGGAGCGCC AGACAAGCTC
    GATGAAGACA CTTGGCAGCA GGAGTGTCCT GTGGTATTAC ACACCTGGAC CAAGGAGTCT
    GCCCACAACT ATGAGAACAA TTGCCATGAG GTGTCTGTCT TCATCAGCCC TGGGGCAACC
    TACTTTGAGG TAGAATTTGA TGAGCGCTGT GAGACTGAGA AGAGGTACGA TTATTTGGAG
    TTCACTGACG CTAGAGGCGG GAAAACACGC TATGACACCA AAGTTGGCAC TGAGAAGTGG
    CCCAGGAAAG TGATCTTCAA GGCTGGCCCC CGGCTGCAGT TCCTCTTTCA CTCTGACAGC
    AGTAACAATG AGTGGGGTTA CAAATTCACC GTCACCGCCT ATGGCCTGCC CGATGTGGCC
    GTGTCCTGGG CCCTGGATTT ACAGCTGCTC GTTTGCCGGC TGATGGGACG CCTCGCTGCC
    CAGTGCATGG CGCTCAAGTC TGCGCACCAG TTTGGAGGCG ACATGGCAGT GGCTCCAGCA
    AAAATGGCGT TGGTCCTGAA CTCTCCGCTG TGGAAACCTG TTTTCAGGCA TCAGTTGAGT
    CCAGAGTTGG GATTAGCAGT GAGCTGGCCC ACGCACCTGC ACCAGGATAG TAAGGAGGTC
    AAGAACATCC CCGATGATCC CTGCCACCAC TTTCTTCTTG AGTTTGCACA GTCAGACCCT
    GCCCAGAACT TCTGTGGGCC ATTTTCAGAA CTTTTCAAAG GATTCATACA GGCATGTAGA
    AAACAGGCCC CAAAGACAGA TATAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC
    ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GGCTTATATG AGAAGCTGCA AAAATATGTA
    AACAGTGGAG GCAACACAGC CCTGAGCGAG GACTTTGCAC AGGTGTATTG CTTGGCAGAT
    GGGATCCGAG TATGGATGCT GGAGATGAAG CAGAAGTCAT TGATGAACCT GGGGAATGAG
    GCAGAAGAAA AGCATGGCCC GGAAGCTGCC GAGGCAAACC CTGAGAGCCT GGCAAAAGAG
    TGTATTCAGA AGAGTCTTCT GTTACTACGA TTTTTGCCCA TGGGAGTAAG TTCAAAAGAA
    AGCTGTGACA AGCTGGTGGC TGCAGTAGAA ACAGATCACC TGCAGCCCTG CGACAGGTGC
    CCAAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGGAGTCTCC CATGGACACA
    GGCACCCTTG CCGCAAGCAG TGGGGGTCCT AGCTCAGATG TGCCGCTGAA GCTGCTGCCC
    AGCGGTGGCC CCCCTGCCAC TGAGGTGTCC TTTGCCACTG CTGAAGAGCC CTCTTCACCT
    TCCGCTCCTG CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TTCGACTGCT CTCCTTCCGG
    TCAGTGGAGG AGGCCAGACC CGTGCCCACC GTGAAGGAGA AATACCCCGT GCTGAAGGAT
    GTCCTGGACT TCATTAAGGA CCAGTCCCTC TCTCATGAGA GTGTCGTAAA GGTTCTTGCC
    TTGAGGAAAG CTCAAGCTCA GAGCATCCTG GAGGTCCTGA GGATCATTCA GTACTGCACG
    GAGTCTCTGG GACGGCCTCA TTGCTTCCAC CCGCCGTGTA TACTTTTCCT GATGGAGCTT
    CTCACCTGCC AGAAAGAGTT TACCAATTAT TTCGCACACA TGGAGGGCTG TGGTGCACGT
    CTGCGCAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT TAAAGCAGTT
    AAAGAATTTA GCAGCATGAA CGACAGGTCG CTGCTGCCTG CCTTGTCCTG TGTGCAGACA
    GCCCTGCTCC ACCTTCTGGA TATGGGCTGG GAACCCGGCG ATCTCGCTTT CTTTGTGGAC
    ATTGGGTTAC CACACCTCCT CATGAAACTG TCACAAGAGA ATATAAGCGT CCACGACAGC
    GTGATCAGCC AGTGCAGTGA AGAAGATGAG CTCGCGGATG CCAAGCAGAA TTCAGAATGG
    ATGGATGAGT GTCAGGATGG CATGTTTGAG GCCTGGTATG AAAAAATAGC CCAGGAAGAT
    CCAGAGAAGC AGAGGAAAAT GCACATGTTC ATTGCTCGCT ACTGCGACCT GCTAAACGTG
    GACATCTCCT GTGATGGCTG TGATGAGATT GCCCCCTGGC ACCGCTACCG GTGTCTGCAG
    TGCAGTGACA TGGATCTCTG CAAAACTTGC TTCCTAGGTG GGGTGAAGCC CGAGGGCCAT
    GGAGATGATC ATGAGATGGT CAGCATGGAG TTTACCTGTG ACCACTGCCA GGGTTTGATC
    ATCGGCCGGA GGATGAACTG CAACGTGTGT GATGACTTCG ATCTTTGCTA CGGGTGCTAC
    GCGGCAAAGA GATACTCTTA CGGCCACTTG CCCACCCACG GCGTCACAGC CCACCCGATG
    GTGACCATCC GGATCAGCGA CCGGCAGAGG CTTATCCAGC CGTACATCCA CAACTACGCC
    TGGCTGCTCT TTGCTGCCCT GGCTCTCTAC AGCGCCCACC TGGCCAGTGC AGAGGACGTG
    GATGGTGAGA AGCTGAATCC CCAGGACCGT GGCAGTGCCA GCCTGCTGCG GAGCCAGTGC
    ATGCAGCTTG TCGGGGACTG TCTGATGAAG GCTCACCAGG GAAAAGGTCT GAAAGCTCTA
    GCTCTCCTTG GTATATTGCC AGATGGTGAA CCTAGTCCAG AAAGTCGGGC CCCACCCGTC
    ACTGTGCCCA CCCACACGGC AGAGGAGGAG AGAGCTGTCT GGGGCCCTGA GGACCTGCCA
    CATACAGGCC TTTCTATGTG CTACAGTGGA AAGGAAGATG GTCCTAAGGA AGTCAGACCT
    TTAGATAGCA GGCAGAGAAG TAAGGCAGAT GAAGAAGGTG CATCCTTAAT GAATGGTCCT
    TCATGCCAGA CTCCAATCTC AGACACACCT GCAGATGGTA GCACCCCTGC AAGGCTTCCA
    GATGCTGAGA ATACAGTCAT GTTGTCTCAG GAGTCCATCG AGGAAAAGGC GGTTACTCCA
    AGTCCTGAGC AGGTGTTTGC TGAGTGCTCC CAGAAGAGGA TTTTAGGATT ACTAGCAGCC
    ATGTTACCTC CCTTAAAGTT GGACCCCACA GTCCCCGTGA TAGACCTGGA GCACGTCCTC
    CCACTCATGT TTCAGGTTGT CATCTCAAAC GTAGGCCACC TGAATGAGAC CTACCACCTC
    ACGCTGGGTC TTCTCGGCCA GCTCATCACC CGCCTCTTAC CAGCGGAGGT AGATGCTGCG
    GTGGTGAAAG TCCTCTCCGC CAAGCATACC TTGTTTGCAA CAGGGGACAG TTCTGTGGTG
    CCAGATGGCT GGAAGACCAC CCACCTGCTC TTTAGCCTGG GAGCCGTGTG TCTGGACAGC
    CGGGTGGGCT TGGACTGGGC ATGCTCCATG GCAGAGATCC TGCGGTCCCT CAACAGTGCC
    CCACTGTGGC GTGACGTCAT CGCCGCCTTC ACAGACCACT GTATCAGGCA GCTGCCTTTC
    CAGCTGAAGC ACACCAACAT CTTCACCCTG CTCGTGCTGG TCGGCTTCCC ACAGGTCCTC
    TGCGTGGGGA CCCGCTGTGT GTATGTGGAT CATGCCAATG AGCCACATAA TGTCATCATC
    TTGAAGCACT TCACGGAGAA GAACAGGGCT GTGATCGTTG ATGTCAAAAC CCGGAAAAGG
    AAAACAGTGA AGGACTACCA GCTGGTCCAG AAAGGACAGG AATGCAGCAG CTCTCAGGCC
    CAGCTGAGCC AGTACTCCCG GCACTTTGCC TTTATCGCCA GTCACCTTCT ACAGACCAGT
    ATGGACAGCC AATGTCCTGA GGCAGTAGAA GCAACATGGG TCCTGTCTCT GGCCCTGAAA
    GGACTGTATA AAACACTAAA GGCTCACAGT TTTGAAGAGA CCCACACCAC CTTCCTTCAG
    ACGGATTTGC TGAAGCTGCT GGTGAAAAAG TGCAGCAAAG GGACTGGTTT CAGTAAAACG
    TGGCTCCTCC GGGATCTGGA GATCCTGTCC ATCATGCTGT ACTCCTCCAA GAAGGAGAGC
    CATACCTCGG CTGAGCACAC AGCCCTGGAG CGAGATGAGA GAGGAGACCA AGAGGAGGAG
    GTGGATGGCA CAGTCAGCAG CCCTGGTGAG CCAGACCAAA AGAAGCTAGA CCCTCTGGAG
    AGCCTGGACG AGCCTACCAG AATATGTTTC TTGATGGCGC ACGATGCGCT CAATGCTCCT
    CTGCACATCC TCCGGGCCAT CTACGAGCTG CAGATGAAAA GGACGGATTC TTTCTTCCTG
    GAGGTTCAGA AGAGGTTTGA TGGGGATGAA CTCACCACAG ATGAGAGGAT TCGCACCCTG
    GCTCAGAGAT GGCAGCCCAG TAGGGGCCTG AGACTGGAGG AACAGAGCAC CAAAGCCGTG
    GATACAGACA TGATCATCTT ACCGTGTTTG TCCCGGCCTG CCCGCTGTGA CCAGGCCACG
    GCCGAATCCA ACCCCGTGAC CCAGAAGCTG ATCTCCAGCA CTGAGAGTGA ATTGCAGCAT
    AGCTACGCCA AACAGCGGCG CAGCAAGAGC ACCGCCCTCC TGCACAAGGA GCTGAATGGC
    AAGAGCAAGC GGGCCATCCG GGAGTACCTG TTCCGCGTGA ACGAAGCAAC AGCTGTCCTG
    TATGCCCGCC ATGTGCTGGC ATCCTTGCTC GCCGAGTGGC CGGGGCACGT ACCAGTGAGC
    GAGGACACCC TGGACCTCAG CGGCCCTGCC CACATGACCT ACATCTTGGA CATGTTCATG
    CAGCTGGAGG AGAAGCACCA GTGGGAGAAG ATCCTGCGGA AGGTGCTTCA GGGCTGCCGA
    GGGAGCATGC TGGGGACCAT GGCTCTGGCC GCCTGCCAGT TCATGGAGGA GCCGGGGATG
    GAGGTGCAAG TGAGAGAGTC AAAGCACCCG TATAACAACA ACACCAGCTT TGAGGATAAA
    GTCCACATCC CTGGTGCAAT CTACCTCTCG ATCAAATTTG ACTCGCAGTG TAGCACAGAG
    GAGGGCTGTG ACGAGCTAGC CATGTCCAGC AGTAGCGACT TCCAGCAGGA TCGACACACT
    TTCAGTGGGT CTCAGCAGAA ATGGAAGGAT TTTGAGCTTC CAGGGGATAC TCTATACTAC
    CGCTTCACCT CTGACATGAG CAACACGGAG TGGGGTTACA AGTTCACTGT GACAGCTGGA
    CACCTGGGCC GATTCCAGAC AGGCTTCGAG ATTTTGAAGC AGATGTTGTC AGAAGAAAGA
    GTTGTGCCAC ACCTCCCACT GGCCAAAATT TGGGAGTGGC TGGTGGGGGT GGCTTGTCGC
    CAGACTGGTC ATCAGCGATT GAAAGCCATC CACCTGCTTC TGAGGATTGT GCAATGCTGC
    ACCCACAGCG ACCTCTGTGA CCTTGCGCTG CTAAAGCCCC TGTGGCAGCT CTTCACTCAC
    ATGGAGTATA GCCTGTTCGA GGATGTGACC TCACCCGGCA TCCTCCTCCC CCTGCACCGT
    GCCCTCACTG AGCTCTTCTT CGTCACCGAG AACCGCGCCC AGGAGCTCGG CTTGCTGCAG
    GAGTATCTGC TGGCCTTGAC CACGGACGAC CACCTTCTCC GCTGCGCGGC ACAGGCCCTG
    CAGAATGTCG CTGCTATCAG CCTGGCCATC AACTACCCAA ACAAGGCAAC CCGCCTCTGG
    AACGTTGAGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06093
    Clone ID Related Accession (Same CDS sequence) XM_005619866.2 , XM_005619866.3
    Accession Version XM_005619866.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8895bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619866.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAAGA ATGTCACCGA GCAGGAACAC AAGCAGTCCC AGCAGCTCAC TTTCCAGTCA
    CTATGCACCT ATTTTAGTGA CAAGGATCCA GGTGGTCTTC TGCTCTTGCC TGAGAAAGGT
    GACCTGGCTG ACATGAACAG CAGCGAAGTT CTGGCCGTCA TGAGCACCCT CCTGTCCGTT
    GCTGCTCGAG AGTGTGAGCT CCTGCTGCTT ACCAGGGCCC AGGGACAGCC CGGCTCCGTG
    CTCTTCTCCC TGTTCTGGGC CGTCCAGGGC AGCCTGCTCT CCTGGTGCTA CCTGCAGCTG
    AAGAGCACCG ACGCGGGAGC CCGGGGTCTC GCCGCCCACC TCATTGACCA GTATGTGGGA
    CAGTTTGTGG CAAGCACGAG AGTGATTCTG GAATCCCTTT TGTCACAGTA TAGTGGACAA
    ACCATTGTAG AGAAACTGTG TAATTCAGTG TTTTCGATGG CAGCTCGTCA ACTGGTGATC
    TTCCTGCTGG ACTTTTGCAC TTTAGACATT TCACACTGTG CACTCCTGAG AGAGTTCAGT
    ACACTGACAG AACTGTTAAA GCAGATCTGC GCGGACCCCA CAGGAGCGCC AGACAAGCTC
    GATGAAGACA CTTGGCAGCA GGAGTGTCCT GTGGTATTAC ACACCTGGAC CAAGGAGTCT
    GCCCACAACT ATGAGAACAA TTGCCATGAG GTGTCTGTCT TCATCAGCCC TGGGGCAACC
    TACTTTGAGG TAGAATTTGA TGAGCGCTGT GAGACTGAGA AGAGGTACGA TTATTTGGAG
    TTCACTGACG CTAGAGGCGG GAAAACACGC TATGACACCA AAGTTGGCAC TGAGAAGTGG
    CCCAGGAAAG TGATCTTCAA GGCTGGCCCC CGGCTGCAGT TCCTCTTTCA CTCTGACAGC
    AGTAACAATG AGTGGGGTTA CAAATTCACC GTCACCGCCT ATGGCCTGCC CGATGTGGCC
    GTGTCCTGGG CCCTGGATTT ACAGCTGCTC GTTTGCCGGC TGATGGGACG CCTCGCTGCC
    CAGTGCATGG CGCTCAAGTC TGCGCACCAG TTTGGAGGCG ACATGGCAGT GGCTCCAGCA
    AAAATGGCGT TGGTCCTGAA CTCTCCGCTG TGGAAACCTG TTTTCAGGCA TCAGTTGAGT
    CCAGAGTTGG GATTAGCAGT GAGCTGGCCC ACGCACCTGC ACCAGGATAG TAAGGAGGTC
    AAGAACATCC CCGATGATCC CTGCCACCAC TTTCTTCTTG AGTTTGCACA GTCAGACCCT
    GCCCAGAACT TCTGTGGGCC ATTTTCAGAA CTTTTCAAAG GATTCATACA GGCATGTAGA
    AAACAGGCCC CAAAGACAGA TATAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC
    ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GGCTTATATG AGAAGCTGCA AAAATATGTA
    AACAGTGGAG GCAACACAGC CCTGAGCGAG GACTTTGCAC AGGTGTATTG CTTGGCAGAT
    GGGATCCGAG TATGGATGCT GGAGATGAAG CAGAAGTCAT TGATGAACCT GGGGAATGAG
    GCAGAAGAAA AGCATGGCCC GGAAGCTGCC GAGGCAAACC CTGAGAGCCT GGCAAAAGAG
    TGTATTCAGA AGAGTCTTCT GTTACTACGA TTTTTGCCCA TGGGAGTAAG TTCAAAAGAA
    AGCTGTGACA AGCTGGTGGC TGCAGTAGAA ACAGATCACC TGCAGCCCTG CGACAGGTGC
    CCAAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGGAGTCTCC CATGGACACA
    GGCACCCTTG CCGCAAGCAG TGGGGGTCCT AGCTCAGATG TGCCGCTGAA GCTGCTGCCC
    AGCGGTGGCC CCCCTGCCAC TGAGGTGTCC TTTGCCACTG CTGAAGAGCC CTCTTCACCT
    TCCGCTCCTG CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TTCGACTGCT CTCCTTCCGG
    TCAGTGGAGG AGGCCAGACC CGTGCCCACC GTGAAGGAGA AATACCCCGT GCTGAAGGAT
    GTCCTGGACT TCATTAAGGA CCAGTCCCTC TCTCATGAGA GTGTCGTAAA GGTTCTTGCC
    TTGAGGAAAG CTCAAGCTCA GAGCATCCTG GAGGTCCTGA GGATCATTCA GTACTGCACG
    GAGTCTCTGG GACGGCCTCA TTGCTTCCAC CCGCCGTGTA TACTTTTCCT GATGGAGCTT
    CTCACCTGCC AGAAAGAGTT TACCAATTAT TTCGCACACA TGGAGGGCTG TGGTGCACGT
    CTGCGCAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT TAAAGCAGTT
    AAAGAATTTA GCAGCATGAA CGACAGGTCG CTGCTGCCTG CCTTGTCCTG TGTGCAGACA
    GCCCTGCTCC ACCTTCTGGA TATGGGCTGG GAACCCGGCG ATCTCGCTTT CTTTGTGGAC
    ATTGGGTTAC CACACCTCCT CATGAAACTG TCACAAGAGA ATATAAGCGT CCACGACAGC
    GTGATCAGCC AGTGCAGTGA AGAAGATGAG CTCGCGGATG CCAAGCAGAA TTCAGAATGG
    ATGGATGAGT GTCAGGATGG CATGTTTGAG GCCTGGTATG AAAAAATAGC CCAGGAAGAT
    CCAGAGAAGC AGAGGAAAAT GCACATGTTC ATTGCTCGCT ACTGCGACCT GCTAAACGTG
    GACATCTCCT GTGATGGCTG TGATGAGATT GCCCCCTGGC ACCGCTACCG GTGTCTGCAG
    TGCAGTGACA TGGATCTCTG CAAAACTTGC TTCCTAGGTG GGGTGAAGCC CGAGGGCCAT
    GGAGATGATC ATGAGATGGT CAGCATGGAG TTTACCTGTG ACCACTGCCA GGGTTTGATC
    ATCGGCCGGA GGATGAACTG CAACGTGTGT GATGACTTCG ATCTTTGCTA CGGGTGCTAC
    GCGGCAAAGA GATACTCTTA CGGCCACTTG CCCACCCACG GCGTCACAGC CCACCCGATG
    GTGACCATCC GGATCAGCGA CCGGCAGAGG CTTATCCAGC CGTACATCCA CAACTACGCC
    TGGCTGCTCT TTGCTGCCCT GGCTCTCTAC AGCGCCCACC TGGCCAGTGC AGAGGACGTG
    GATGGTGAGA AGCTGAATCC CCAGGACCGT GGCAGTGCCA GCCTGCTGCG GAGCCAGTGC
    ATGCAGCTTG TCGGGGACTG TCTGATGAAG GCTCACCAGG GAAAAGGTCT GAAAGCTCTA
    GCTCTCCTTG GTATATTGCC AGATGGTGAA CCTAGTCCAG AAAGTCGGGC CCCACCCGTC
    ACTGTGCCCA CCCACACGGC AGAGGAGGAG AGAGCTGTCT GGGGCCCTGA GGACCTGCCA
    CATACAGGCC TTTCTATGTG CTACAGTGGA AAGGAAGATG GTCCTAAGGA AGTCAGACCT
    TTAGATAGCA GGCAGAGAAG TAAGGCAGAT GAAGAAGGTG CATCCTTAAT GAATGGTCCT
    TCATGCCAGA CTCCAATCTC AGACACACCT GCAGATGGTA GCACCCCTGC AAGGCTTCCA
    GATGCTGAGA ATACAGTCAT GTTGTCTCAG GAGTCCATCG AGGAAAAGGC GGTTACTCCA
    AGTCCTGAGC AGGTGTTTGC TGAGTGCTCC CAGAAGAGGA TTTTAGGATT ACTAGCAGCC
    ATGTTACCTC CCTTAAAGTT GGACCCCACA GTCCCCGTGA TAGACCTGGA GCACGTCCTC
    CCACTCATGT TTCAGGTTGT CATCTCAAAC GTAGGCCACC TGAATGAGAC CTACCACCTC
    ACGCTGGGTC TTCTCGGCCA GCTCATCACC CGCCTCTTAC CAGCGGAGGT AGATGCTGCG
    GTGGTGAAAG TCCTCTCCGC CAAGCATACC TTGTTTGCAA CAGGGGACAG TTCTGTGGTG
    CCAGATGGCT GGAAGACCAC CCACCTGCTC TTTAGCCTGG GAGCCGTGTG TCTGGACAGC
    CGGGTGGGCT TGGACTGGGC ATGCTCCATG GCAGAGATCC TGCGGTCCCT CAACAGTGCC
    CCACTGTGGC GTGACGTCAT CGCCGCCTTC ACAGACCACT GTATCAGGCA GCTGCCTTTC
    CAGCTGAAGC ACACCAACAT CTTCACCCTG CTCGTGCTGG TCGGCTTCCC ACAGGTCCTC
    TGCGTGGGGA CCCGCTGTGT GTATGTGGAT CATGCCAATG AGCCACATAA TGTCATCATC
    TTGAAGCACT TCACGGAGAA GAACAGGGCT GTGATCGTTG ATGTCAAAAC CCGGAAAAGG
    AAAACAGTGA AGGACTACCA GCTGGTCCAG AAAGGACAGG AATGCAGCAG CTCTCAGGCC
    CAGCTGAGCC AGTACTCCCG GCACTTTGCC TTTATCGCCA GTCACCTTCT ACAGACCAGT
    ATGGACAGCC AATGTCCTGA GGCAGTAGAA GCAACATGGG TCCTGTCTCT GGCCCTGAAA
    GGACTGTATA AAACACTAAA GGCTCACAGT TTTGAAGAGA CCCACACCAC CTTCCTTCAG
    ACGGATTTGC TGAAGCTGCT GGTGAAAAAG TGCAGCAAAG GGACTGGTTT CAGTAAAACG
    TGGCTCCTCC GGGATCTGGA GATCCTGTCC ATCATGCTGT ACTCCTCCAA GAAGGAGAGC
    CATACCTCGG CTGAGCACAC AGCCCTGGAG CGAGATGAGA GAGGAGACCA AGAGGAGGAG
    GTGGATGGCA CAGTCAGCAG CCCTGGTGAG CCAGACCAAA AGAAGCTAGA CCCTCTGGAG
    AGCCTGGACG AGCCTACCAG AATATGTTTC TTGATGGCGC ACGATGCGCT CAATGCTCCT
    CTGCACATCC TCCGGGCCAT CTACGAGCTG CAGATGAAAA GGACGGATTC TTTCTTCCTG
    GAGGTTCAGA AGAGGTTTGA TGGGGATGAA CTCACCACAG ATGAGAGGAT TCGCACCCTG
    GCTCAGAGAT GGCAGCCCAG TAGGGGCCTG AGACTGGAGG AACAGAGCAC CAAAGCCGTG
    GATACAGACA TGATCATCTT ACCGTGTTTG TCCCGGCCTG CCCGCTGTGA CCAGGCCACG
    GCCGAATCCA ACCCCGTGAC CCAGAAGCTG ATCTCCAGCA CTGAGAGTGA ATTGCAGCAT
    AGCTACGCCA AACAGCGGCG CAGCAAGAGC ACCGCCCTCC TGCACAAGGA GCTGAATGGC
    AAGAGCAAGC GGGCCATCCG GGAGTACCTG TTCCGCGTGA ACGAAGCAAC AGCTGTCCTG
    TATGCCCGCC ATGTGCTGGC ATCCTTGCTC GCCGAGTGGC CGGGGCACGT ACCAGTGAGC
    GAGGACACCC TGGACCTCAG CGGCCCTGCC CACATGACCT ACATCTTGGA CATGTTCATG
    CAGCTGGAGG AGAAGCACCA GTGGGAGAAG ATCCTGCGGA AGGTGCTTCA GGGCTGCCGA
    GGGAGCATGC TGGGGACCAT GGCTCTGGCC GCCTGCCAGT TCATGGAGGA GCCGGGGATG
    GAGGTGCAAG TGAGAGAGTC AAAGCACCCG TATAACAACA ACACCAGCTT TGAGGATAAA
    GTCCACATCC CTGGTGCAAT CTACCTCTCG ATCAAATTTG ACTCGCAGTG TAGCACAGAG
    GAGGGCTGTG ACGAGCTAGC CATGTCCAGC AGTAGCGACT TCCAGCAGGA TCGACACACT
    TTCAGTGGGT CTCAGCAGAA ATGGAAGGAT TTTGAGCTTC CAGGGGATAC TCTATACTAC
    CGCTTCACCT CTGACATGAG CAACACGGAG TGGGGTTACA AGTTCACTGT GACAGCTGGA
    CACCTGGGCC GATTCCAGAC AGGCTTCGAG ATTTTGAAGC AGATGTTGTC AGAAGAAAGA
    GTTGTGCCAC ACCTCCCACT GGCCAAAATT TGGGAGTGGC TGGTGGGGGT GGCTTGTCGC
    CAGACTGGTC ATCAGCGATT GAAAGCCATC CACCTGCTTC TGAGGATTGT GCAATGCTGC
    ACCCACAGCG ACCTCTGTGA CCTTGCGCTG CTAAAGCCCC TGTGGCAGCT CTTCACTCAC
    ATGGAGTATA GCCTGTTCGA GGATGTGACC TCACCCGGCA TCCTCCTCCC CCTGCACCGT
    GCCCTCACTG AGCTCTTCTT CGTCACCGAG AACCGCGCCC AGGAGCTCGG CTTGCTGCAG
    GAGTATCTGC TGGCCTTGAC CACGGACGAC CACCTTCTCC GCTGCGCGGC ACAGGCCCTG
    CAGAATGTCG CTGCTATCAG CCTGGCCATC AACTACCCAA ACAAGGCAAC CCGCCTCTGG
    AACGTTGAGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619923.1
    CDS1..8895
    Translation

    Target ORF information:

    RefSeq Version XM_005619866.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619866.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    ATGGGGAACG CTCCGAGTCA CAGCAGTGAA GACGAAGCGG GTGCCGCCGG GGGAGAGGGC 
    TGGGGCCCGC ACCAGGACTG GGCTGCGGAC GCGAGCGCCA TCCCCGGCTC GGGCCCCGCG
    GCCCCAGCGC TGCCCCCCGC CGCCGCGCTG CTGGAGCCGG CCCGACTGCG AGAGGCGGCG
    GCGGCGCTGC GGCCCGCGCC GCCTTGCGAG TCTCTGGTGT CCCGGCACCA CGGCGCGCTG
    TTGCGCTGGC TGGAGGAGCG GCTGGGCCGC GGCGAAGAGG CCGTCACCCT GGAGCAGTTC
    CGCGAGCTGC TGGAGGCTCG CGGCGCCGGC TGCTCGGGCG AGCAGTTCGA GGAGGCCTTT
    GCCCAGTTTG ATGCTGAGGG CGATGGTACT GTTGATGCTG AGAACATGCT GGAGACCCTG
    AAGAATTCCA GTGGAGCCAA TCTCCAGGGG GAGCTGAGCC ACATCATCAG ACAGCTGCAG
    GCCTGTTCTC TTGTCCCAGG TTTCATAGAC ATATTCTCAG AGTCCAAGGA GGGTCTGGGT
    GTGCACGCAT CCTTGATCCT GCGTTTCCTG CACCGCAATC GGCTCTCCGG CATGGCCATC
    CCCTGCCCCA TGCTGGACCA CTGCAGTAAC ATGTGCTCCA TGAGGGCTTC TGTTCTGAAG
    GAGTCTTTGG ACCAGCTGGT ACAGAGAGAA AAGGAAAGTC CTGGAGATCT AACCAGAAGC
    CCAGAGATGG ATAAACTCAA GTCAGTAGCA AAGTGCTACG CTTACATAGA AACATCCTCC
    AACCCTGCAG ACATCGACAA GATGACAAAT GGAGAAACAT CATCATACTG GCAATCAGAT
    GGCAGTGCTC GCTCACACTG GATTCGCTTA AAGATGAAGC CAGATGTTGT GCTTAGGCAC
    TTGTCCATTG CTGTGGCTGC CACTGACCAG AGCTACATGC CGCAGCAGGT GACGGTCGCT
    GTGGGCAGAA GTGCCAGCGA TCTTCAGGAT GTCCGCGATG TGCACATTCC CAGCAATGTC
    ACTGGCTATG TGACACTTCT GGAGAACGCC AACATTAGTC AGCTGTATGT CCAGATTAAC
    ATAAAACGTT GTCTTAGCGA TGGATGTGAC ACTAGAATTC ATGGTCTGAG GGCTGTTGGC
    TTTCAGAGAG TTAAGAAGTC TGGGGTCTCC GTGTCAGATG CTTCTGCAAT CTGGTACTGG
    TCTCTGCTGA CCTCTCTGGT GACGGCGTCC ATGGAGACCA ACCCTGCCTT CGTCCAGACA
    GTGCTGCACA ACACGCAGAA GGCACTACAG CACATGCCTC CACTTTCCCT CTCACCAGGA
    TCTACAGATG TCTCAACTTT CCTGTCTCCC AACGTTCTAG AAGAGGTGGA CAGCTTCCTC
    ATAAGGATAA CTAGCTGCTG TTCAACTCCA GAGGTGGAGC TGACTCTTCT GGCCTTTGCA
    CTAGCAAGAG GAAGTGTTGC CAAAGTGCTG AGCTGTCTGT GTACCATTGC TGACCACCTG
    GACACACGCT ATGATGCCTC GTCCCTCATC TCCTCCATGG CATCAGTCAG GCAGACCCTG
    CTCCTTAAAT ACGGTAAACC TCTTCAGCTG ACCCTTCAGG CCTGTGATGT TAAAGGGAAA
    GAAGAGAAAT CAGGGCCCGA AAACCTCCTT GTCGAGCCGT GGACAAGAGA TGGGTTTCTT
    ACAGAGACGG GAAAGACCAG AGCAAGTACC ATTTTTTCTG CAGGAACTGA ATCTGCCTTC
    CAAGTTACAC AGATCAGGAT TATGGTTCGA CGTGGTGGCA TCGGTGCCCA ATGTGGGTTG
    GTGTTTGCCT ATAACTCATC TTCTGAAAAA TTTCATGCCG AAGAACACTT CAAAAGGTTT
    GAAAAATATG ACAAGTGGAA GCTTCAGGAA TTCAGGCAAT TTGTAAAGAG CAGGGTTGGT
    TGCTCAGCTG ATGACCTTGG CGAGGATGAC CCTATTGGCT GGTTTGAGCT AGAAGAAGAA
    TGGGATGAAG CAGATGTCAA GTTGCAGCAG TGCAGAGTTG CCAAATATTT GATGGTGAAG
    TTCCTCTGCA CCCGTCAGGA GTCGGCAGAG CGCTTGGGAG TGCAAGGTCT CAGCATCAGC
    GGGTATGTCC GGCCAATGCG AGTGGAAGCG GAGCAGCACA TTGTCTGTGC CCACTGCAGA
    AAGGATGGGG AGGAGAGTGT CTGCGGGACC ACGCTACTCC TCAGGACGCT TCAGTTCACT
    CAGCAGCTTG CCCATGACCT GGTGCAGCAG AAGGAAAGTG GCTTAAAATA TAAATCTTTT
    CTGGACTTCG CGGGTCTTGA TTTGCAGATC TTCTGGAATT TTTATAGTAA ATTAAAGCAA
    AACCCGAGGG AAGAATGCAT CTGTGCCCAG ACCCTGCTTC TGCGGCTCCT GCAGAGCTGC
    TTGTCAGTGC TGCAGAGAGA CGGGCCAGCC ACCCCCGAGG AGGCCAAGGC TCCCCGGCAG
    AGCCCTGGAG GGGCGGGGGC TGCCAAGGAA CTCCACGCAC ACTTATGCGA CGTGGTGGAT
    AGGGTGGACG GAGACTCTGT GCCCATGGAA ACATTAAAAC AAGAAGTCAG GAACACCCTT
    CTCAGTGGGG CTGCCGTCTT TTTTCCTGAT CGACAGAGCC GGAGGAGCCA TCTCTTCACC
    ATGATGAAGA ATGTCACCGA GCAGGAACAC AAGCAGTCCC AGCAGCTCAC TTTCCAGTCA
    CTATGCACCT ATTTTAGTGA CAAGGATCCA GGTGGTCTTC TGCTCTTGCC TGAGAAAGGT
    GACCTGGCTG ACATGAACAG CAGCGAAGTT CTGGCCGTCA TGAGCACCCT CCTGTCCGTT
    GCTGCTCGAG AGTGTGAGCT CCTGCTGCTT ACCAGGGCCC AGGGACAGCC CGGCTCCGTG
    CTCTTCTCCC TGTTCTGGGC CGTCCAGGGC AGCCTGCTCT CCTGGTGCTA CCTGCAGCTG
    AAGAGCACCG ACGCGGGAGC CCGGGGTCTC GCCGCCCACC TCATTGACCA GTATGTGGGA
    CAGTTTGTGG CAAGCACGAG AGTGATTCTG GAATCCCTTT TGTCACAGTA TAGTGGACAA
    ACCATTGTAG AGAAACTGTG TAATTCAGTG TTTTCGATGG CAGCTCGTCA ACTGGTGATC
    TTCCTGCTGG ACTTTTGCAC TTTAGACATT TCACACTGTG CACTCCTGAG AGAGTTCAGT
    ACACTGACAG AACTGTTAAA GCAGATCTGC GCGGACCCCA CAGGAGCGCC AGACAAGCTC
    GATGAAGACA CTTGGCAGCA GGAGTGTCCT GTGGTATTAC ACACCTGGAC CAAGGAGTCT
    GCCCACAACT ATGAGAACAA TTGCCATGAG GTGTCTGTCT TCATCAGCCC TGGGGCAACC
    TACTTTGAGG TAGAATTTGA TGAGCGCTGT GAGACTGAGA AGAGGTACGA TTATTTGGAG
    TTCACTGACG CTAGAGGCGG GAAAACACGC TATGACACCA AAGTTGGCAC TGAGAAGTGG
    CCCAGGAAAG TGATCTTCAA GGCTGGCCCC CGGCTGCAGT TCCTCTTTCA CTCTGACAGC
    AGTAACAATG AGTGGGGTTA CAAATTCACC GTCACCGCCT ATGGCCTGCC CGATGTGGCC
    GTGTCCTGGG CCCTGGATTT ACAGCTGCTC GTTTGCCGGC TGATGGGACG CCTCGCTGCC
    CAGTGCATGG CGCTCAAGTC TGCGCACCAG TTTGGAGGCG ACATGGCAGT GGCTCCAGCA
    AAAATGGCGT TGGTCCTGAA CTCTCCGCTG TGGAAACCTG TTTTCAGGCA TCAGTTGAGT
    CCAGAGTTGG GATTAGCAGT GAGCTGGCCC ACGCACCTGC ACCAGGATAG TAAGGAGGTC
    AAGAACATCC CCGATGATCC CTGCCACCAC TTTCTTCTTG AGTTTGCACA GTCAGACCCT
    GCCCAGAACT TCTGTGGGCC ATTTTCAGAA CTTTTCAAAG GATTCATACA GGCATGTAGA
    AAACAGGCCC CAAAGACAGA TATAGTTGCT GGTTCCACTA TTGATCAAGC TGTGAACGCC
    ACCTTTGCTG CTCTGGTGTG TCGTACTCCA GGCTTATATG AGAAGCTGCA AAAATATGTA
    AACAGTGGAG GCAACACAGC CCTGAGCGAG GACTTTGCAC AGGTGTATTG CTTGGCAGAT
    GGGATCCGAG TATGGATGCT GGAGATGAAG CAGAAGTCAT TGATGAACCT GGGGAATGAG
    GCAGAAGAAA AGCATGGCCC GGAAGCTGCC GAGGCAAACC CTGAGAGCCT GGCAAAAGAG
    TGTATTCAGA AGAGTCTTCT GTTACTACGA TTTTTGCCCA TGGGAGTAAG TTCAAAAGAA
    AGCTGTGACA AGCTGGTGGC TGCAGTAGAA ACAGATCACC TGCAGCCCTG CGACAGGTGC
    CCAAGGACGA GCTCTGTGGT GGAGGAGCAT TTCCAGGCCT CGGAGTCTCC CATGGACACA
    GGCACCCTTG CCGCAAGCAG TGGGGGTCCT AGCTCAGATG TGCCGCTGAA GCTGCTGCCC
    AGCGGTGGCC CCCCTGCCAC TGAGGTGTCC TTTGCCACTG CTGAAGAGCC CTCTTCACCT
    TCCGCTCCTG CCCGGCGGCC TCCCTTCACC CGAGGGCGAC TTCGACTGCT CTCCTTCCGG
    TCAGTGGAGG AGGCCAGACC CGTGCCCACC GTGAAGGAGA AATACCCCGT GCTGAAGGAT
    GTCCTGGACT TCATTAAGGA CCAGTCCCTC TCTCATGAGA GTGTCGTAAA GGTTCTTGCC
    TTGAGGAAAG CTCAAGCTCA GAGCATCCTG GAGGTCCTGA GGATCATTCA GTACTGCACG
    GAGTCTCTGG GACGGCCTCA TTGCTTCCAC CCGCCGTGTA TACTTTTCCT GATGGAGCTT
    CTCACCTGCC AGAAAGAGTT TACCAATTAT TTCGCACACA TGGAGGGCTG TGGTGCACGT
    CTGCGCAAAG AGATTCGAGA CACTTACTAC CAGTTTGTTC TGTTTTTGGT TAAAGCAGTT
    AAAGAATTTA GCAGCATGAA CGACAGGTCG CTGCTGCCTG CCTTGTCCTG TGTGCAGACA
    GCCCTGCTCC ACCTTCTGGA TATGGGCTGG GAACCCGGCG ATCTCGCTTT CTTTGTGGAC
    ATTGGGTTAC CACACCTCCT CATGAAACTG TCACAAGAGA ATATAAGCGT CCACGACAGC
    GTGATCAGCC AGTGCAGTGA AGAAGATGAG CTCGCGGATG CCAAGCAGAA TTCAGAATGG
    ATGGATGAGT GTCAGGATGG CATGTTTGAG GCCTGGTATG AAAAAATAGC CCAGGAAGAT
    CCAGAGAAGC AGAGGAAAAT GCACATGTTC ATTGCTCGCT ACTGCGACCT GCTAAACGTG
    GACATCTCCT GTGATGGCTG TGATGAGATT GCCCCCTGGC ACCGCTACCG GTGTCTGCAG
    TGCAGTGACA TGGATCTCTG CAAAACTTGC TTCCTAGGTG GGGTGAAGCC CGAGGGCCAT
    GGAGATGATC ATGAGATGGT CAGCATGGAG TTTACCTGTG ACCACTGCCA GGGTTTGATC
    ATCGGCCGGA GGATGAACTG CAACGTGTGT GATGACTTCG ATCTTTGCTA CGGGTGCTAC
    GCGGCAAAGA GATACTCTTA CGGCCACTTG CCCACCCACG GCGTCACAGC CCACCCGATG
    GTGACCATCC GGATCAGCGA CCGGCAGAGG CTTATCCAGC CGTACATCCA CAACTACGCC
    TGGCTGCTCT TTGCTGCCCT GGCTCTCTAC AGCGCCCACC TGGCCAGTGC AGAGGACGTG
    GATGGTGAGA AGCTGAATCC CCAGGACCGT GGCAGTGCCA GCCTGCTGCG GAGCCAGTGC
    ATGCAGCTTG TCGGGGACTG TCTGATGAAG GCTCACCAGG GAAAAGGTCT GAAAGCTCTA
    GCTCTCCTTG GTATATTGCC AGATGGTGAA CCTAGTCCAG AAAGTCGGGC CCCACCCGTC
    ACTGTGCCCA CCCACACGGC AGAGGAGGAG AGAGCTGTCT GGGGCCCTGA GGACCTGCCA
    CATACAGGCC TTTCTATGTG CTACAGTGGA AAGGAAGATG GTCCTAAGGA AGTCAGACCT
    TTAGATAGCA GGCAGAGAAG TAAGGCAGAT GAAGAAGGTG CATCCTTAAT GAATGGTCCT
    TCATGCCAGA CTCCAATCTC AGACACACCT GCAGATGGTA GCACCCCTGC AAGGCTTCCA
    GATGCTGAGA ATACAGTCAT GTTGTCTCAG GAGTCCATCG AGGAAAAGGC GGTTACTCCA
    AGTCCTGAGC AGGTGTTTGC TGAGTGCTCC CAGAAGAGGA TTTTAGGATT ACTAGCAGCC
    ATGTTACCTC CCTTAAAGTT GGACCCCACA GTCCCCGTGA TAGACCTGGA GCACGTCCTC
    CCACTCATGT TTCAGGTTGT CATCTCAAAC GTAGGCCACC TGAATGAGAC CTACCACCTC
    ACGCTGGGTC TTCTCGGCCA GCTCATCACC CGCCTCTTAC CAGCGGAGGT AGATGCTGCG
    GTGGTGAAAG TCCTCTCCGC CAAGCATACC TTGTTTGCAA CAGGGGACAG TTCTGTGGTG
    CCAGATGGCT GGAAGACCAC CCACCTGCTC TTTAGCCTGG GAGCCGTGTG TCTGGACAGC
    CGGGTGGGCT TGGACTGGGC ATGCTCCATG GCAGAGATCC TGCGGTCCCT CAACAGTGCC
    CCACTGTGGC GTGACGTCAT CGCCGCCTTC ACAGACCACT GTATCAGGCA GCTGCCTTTC
    CAGCTGAAGC ACACCAACAT CTTCACCCTG CTCGTGCTGG TCGGCTTCCC ACAGGTCCTC
    TGCGTGGGGA CCCGCTGTGT GTATGTGGAT CATGCCAATG AGCCACATAA TGTCATCATC
    TTGAAGCACT TCACGGAGAA GAACAGGGCT GTGATCGTTG ATGTCAAAAC CCGGAAAAGG
    AAAACAGTGA AGGACTACCA GCTGGTCCAG AAAGGACAGG AATGCAGCAG CTCTCAGGCC
    CAGCTGAGCC AGTACTCCCG GCACTTTGCC TTTATCGCCA GTCACCTTCT ACAGACCAGT
    ATGGACAGCC AATGTCCTGA GGCAGTAGAA GCAACATGGG TCCTGTCTCT GGCCCTGAAA
    GGACTGTATA AAACACTAAA GGCTCACAGT TTTGAAGAGA CCCACACCAC CTTCCTTCAG
    ACGGATTTGC TGAAGCTGCT GGTGAAAAAG TGCAGCAAAG GGACTGGTTT CAGTAAAACG
    TGGCTCCTCC GGGATCTGGA GATCCTGTCC ATCATGCTGT ACTCCTCCAA GAAGGAGAGC
    CATACCTCGG CTGAGCACAC AGCCCTGGAG CGAGATGAGA GAGGAGACCA AGAGGAGGAG
    GTGGATGGCA CAGTCAGCAG CCCTGGTGAG CCAGACCAAA AGAAGCTAGA CCCTCTGGAG
    AGCCTGGACG AGCCTACCAG AATATGTTTC TTGATGGCGC ACGATGCGCT CAATGCTCCT
    CTGCACATCC TCCGGGCCAT CTACGAGCTG CAGATGAAAA GGACGGATTC TTTCTTCCTG
    GAGGTTCAGA AGAGGTTTGA TGGGGATGAA CTCACCACAG ATGAGAGGAT TCGCACCCTG
    GCTCAGAGAT GGCAGCCCAG TAGGGGCCTG AGACTGGAGG AACAGAGCAC CAAAGCCGTG
    GATACAGACA TGATCATCTT ACCGTGTTTG TCCCGGCCTG CCCGCTGTGA CCAGGCCACG
    GCCGAATCCA ACCCCGTGAC CCAGAAGCTG ATCTCCAGCA CTGAGAGTGA ATTGCAGCAT
    AGCTACGCCA AACAGCGGCG CAGCAAGAGC ACCGCCCTCC TGCACAAGGA GCTGAATGGC
    AAGAGCAAGC GGGCCATCCG GGAGTACCTG TTCCGCGTGA ACGAAGCAAC AGCTGTCCTG
    TATGCCCGCC ATGTGCTGGC ATCCTTGCTC GCCGAGTGGC CGGGGCACGT ACCAGTGAGC
    GAGGACACCC TGGACCTCAG CGGCCCTGCC CACATGACCT ACATCTTGGA CATGTTCATG
    CAGCTGGAGG AGAAGCACCA GTGGGAGAAG ATCCTGCGGA AGGTGCTTCA GGGCTGCCGA
    GGGAGCATGC TGGGGACCAT GGCTCTGGCC GCCTGCCAGT TCATGGAGGA GCCGGGGATG
    GAGGTGCAAG TGAGAGAGTC AAAGCACCCG TATAACAACA ACACCAGCTT TGAGGATAAA
    GTCCACATCC CTGGTGCAAT CTACCTCTCG ATCAAATTTG ACTCGCAGTG TAGCACAGAG
    GAGGGCTGTG ACGAGCTAGC CATGTCCAGC AGTAGCGACT TCCAGCAGGA TCGACACACT
    TTCAGTGGGT CTCAGCAGAA ATGGAAGGAT TTTGAGCTTC CAGGGGATAC TCTATACTAC
    CGCTTCACCT CTGACATGAG CAACACGGAG TGGGGTTACA AGTTCACTGT GACAGCTGGA
    CACCTGGGCC GATTCCAGAC AGGCTTCGAG ATTTTGAAGC AGATGTTGTC AGAAGAAAGA
    GTTGTGCCAC ACCTCCCACT GGCCAAAATT TGGGAGTGGC TGGTGGGGGT GGCTTGTCGC
    CAGACTGGTC ATCAGCGATT GAAAGCCATC CACCTGCTTC TGAGGATTGT GCAATGCTGC
    ACCCACAGCG ACCTCTGTGA CCTTGCGCTG CTAAAGCCCC TGTGGCAGCT CTTCACTCAC
    ATGGAGTATA GCCTGTTCGA GGATGTGACC TCACCCGGCA TCCTCCTCCC CCTGCACCGT
    GCCCTCACTG AGCTCTTCTT CGTCACCGAG AACCGCGCCC AGGAGCTCGG CTTGCTGCAG
    GAGTATCTGC TGGCCTTGAC CACGGACGAC CACCTTCTCC GCTGCGCGGC ACAGGCCCTG
    CAGAATGTCG CTGCTATCAG CCTGGCCATC AACTACCCAA ACAAGGCAAC CCGCCTCTGG
    AACGTTGAGT GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe288036
    Clone ID Related Accession (Same CDS sequence) XM_022418395.1
    Accession Version XM_022418395.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8490bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product zinc finger ZZ-type and EF-hand domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCTGGAGA CCCTGAAGAA TTCCAGTGGA GCCAATCTCC AGGGGGAGCT GAGCCACATC 
    ATCAGACAGC TGCAGGCCTG TTCTCTTGTC CCAGGTTTCA TAGACATATT CTCAGAGTCC
    AAGGAGGGTC TGGGTGTGCA CGCATCCTTG ATCCTGCGTT TCCTGCACCG CAATCGGCTC
    TCCGGCATGG CCATCCCCTG CCCCATGCTG GACCACTGCA GTAACATGTG CTCCATGAGG
    GCTTCTGTTC TGAAGGAGTC TTTGGACCAG CTGGTACAGA GAGAAAAGGA AAGTCCTGGA
    GATCTAACCA GAAGCCCAGA GATGGATAAA CTCAAGTCAG TAGCAAAGTG CTACGCTTAC
    ATAGAAACAT CCTCCAACCC TGCAGACATC GACAAGATGA CAAATGGAGA AACATCATCA
    TACTGGCAAT CAGATGGCAG TGCTCGCTCA CACTGGATTC GCTTAAAGAT GAAGCCAGAT
    GTTGTGCTTA GGCACTTGTC CATTGCTGTG GCTGCCACTG ACCAGAGCTA CATGCCGCAG
    CAGGTGACGG TCGCTGTGGG CAGAAGTGCC AGCGATCTTC AGGATGTCCG CGATGTGCAC
    ATTCCCAGCA ATGTCACTGG CTATGTGACA CTTCTGGAGA ACGCCAACAT TAGTCAGCTG
    TATGTCCAGA TTAACATAAA ACGTTGTCTT AGCGATGGAT GTGACACTAG AATTCATGGT
    CTGAGGGCTG TTGGCTTTCA GAGAGTTAAG AAGTCTGGGG TCTCCGTGTC AGATGCTTCT
    GCAATCTGGT ACTGGTCTCT GCTGACCTCT CTGGTGACGG CGTCCATGGA GACCAACCCT
    GCCTTCGTCC AGACAGTGCT GCACAACACG CAGAAGGCAC TACAGCACAT GCCTCCACTT
    TCCCTCTCAC CAGGATCTAC AGATGTCTCA ACTTTCCTGT CTCCCAACGT TCTAGAAGAG
    GTGGACAGCT TCCTCATAAG GATAACTAGC TGCTGTTCAA CTCCAGAGGT GGAGCTGACT
    CTTCTGGCCT TTGCACTAGC AAGAGGAAGT GTTGCCAAAG TGCTGAGCTG TCTGTGTACC
    ATTGCTGACC ACCTGGACAC ACGCTATGAT GCCTCGTCCC TCATCTCCTC CATGGCATCA
    GTCAGGCAGA CCCTGCTCCT TAAATACGGT AAACCTCTTC AGCTGACCCT TCAGGCCTGT
    GATGTTAAAG GGAAAGAAGA GAAATCAGGG CCCGAAAACC TCCTTGTCGA GCCGTGGACA
    AGAGATGGGT TTCTTACAGA GACGGGAAAG ACCAGAGCAA GTACCATTTT TTCTGCAGGA
    ACTGAATCTG CCTTCCAAGT TACACAGATC AGGATTATGG TTCGACGTGG TGGCATCGGT
    GCCCAATGTG GGTTGGTGTT TGCCTATAAC TCATCTTCTG AAAAATTTCA TGCCGAAGAA
    CACTTCAAAA GGTTTGAAAA ATATGACAAG TGGAAGCTTC AGGAATTCAG GCAATTTGTA
    AAGAGCAGGG TTGGTTGCTC AGCTGATGAC CTTGGCGAGG ATGACCCTAT TGGCTGGTTT
    GAGCTAGAAG AAGAATGGGA TGAAGCAGAT GTCAAGTTGC AGCAGTGCAG AGTTGCCAAA
    TATTTGATGG TGAAGTTCCT CTGCACCCGT CAGGAGTCGG CAGAGCGCTT GGGAGTGCAA
    GGTCTCAGCA TCAGCGGGTA TGTCCGGCCA ATGCGAGTGG AAGCGGAGCA GCACATTGTC
    TGTGCCCACT GCAGAAAGGA TGGGGAGGAG AGTGTCTGCG GGACCACGCT ACTCCTCAGG
    ACGCTTCAGT TCACTCAGCA GCTTGCCCAT GACCTGGTGC AGCAGAAGGA AAGTGGCTTA
    AAATATAAAT CTTTTCTGGA CTTCGCGGGT CTTGATTTGC AGATCTTCTG GAATTTTTAT
    AGTAAATTAA AGCAAAACCC GAGGGAAGAA TGCATCTGTG CCCAGACCCT GCTTCTGCGG
    CTCCTGCAGA GCTGCTTGTC AGTGCTGCAG AGAGACGGGC CAGCCACCCC CGAGGAGGCC
    AAGGCTCCCC GGCAGAGCCC TGGAGGGGCG GGGGCTGCCA AGGAACTCCA CGCACACTTA
    TGCGACGTGG TGGATAGGGT GGACGGAGAC TCTGTGCCCA TGGAAACATT AAAACAAGAA
    GTCAGGAACA CCCTTCTCAG TGGGGCTGCC GTCTTTTTTC CTGATCGACA GAGCCGGAGG
    AGCCATCTCT TCACCATGAT GAAGAATGTC ACCGAGCAGG AACACAAGCA GTCCCAGCAG
    CTCACTTTCC AGTCACTATG CACCTATTTT AGTGACAAGG ATCCAGGTGG TCTTCTGCTC
    TTGCCTGAGA AAGGTGACCT GGCTGACATG AACAGCAGCG AAGTTCTGGC CGTCATGAGC
    ACCCTCCTGT CCGTTGCTGC TCGAGAGTGT GAGCTCCTGC TGCTTACCAG GGCCCAGGGA
    CAGCCCGGCT CCGTGCTCTT CTCCCTGTTC TGGGCCGTCC AGGGCAGCCT GCTCTCCTGG
    TGCTACCTGC AGCTGAAGAG CACCGACGCG GGAGCCCGGG GTCTCGCCGC CCACCTCATT
    GACCAGTATG TGGGACAGTT TGTGGCAAGC ACGAGAGTGA TTCTGGAATC CCTTTTGTCA
    CAGTATAGTG GACAAACCAT TGTAGAGAAA CTGTGTAATT CAGTGTTTTC GATGGCAGCT
    CGTCAACTGG TGATCTTCCT GCTGGACTTT TGCACTTTAG ACATTTCACA CTGTGCACTC
    CTGAGAGAGT TCAGTACACT GACAGAACTG TTAAAGCAGA TCTGCGCGGA CCCCACAGGA
    GCGCCAGACA AGCTCGATGA AGACACTTGG CAGCAGGAGT GTCCTGTGGT ATTACACACC
    TGGACCAAGG AGTCTGCCCA CAACTATGAG AACAATTGCC ATGAGGTGTC TGTCTTCATC
    AGCCCTGGGG CAACCTACTT TGAGGTAGAA TTTGATGAGC GCTGTGAGAC TGAGAAGAGG
    TACGATTATT TGGAGTTCAC TGACGCTAGA GGCGGGAAAA CACGCTATGA CACCAAAGTT
    GGCACTGAGA AGTGGCCCAG GAAAGTGATC TTCAAGGCTG GCCCCCGGCT GCAGTTCCTC
    TTTCACTCTG ACAGCAGTAA CAATGAGTGG GGTTACAAAT TCACCGTCAC CGCCTATGGC
    CTGCCCGATG TGGCCGTGTC CTGGGCCCTG GATTTACAGC TGCTCGTTTG CCGGCTGATG
    GGACGCCTCG CTGCCCAGTG CATGGCGCTC AAGTCTGCGC ACCAGTTTGG AGGCGACATG
    GCAGTGGCTC CAGCAAAAAT GGCGTTGGTC CTGAACTCTC CGCTGTGGAA ACCTGTTTTC
    AGGCATCAGT TGAGTCCAGA GTTGGGATTA GCAGTGAGCT GGCCCACGCA CCTGCACCAG
    GATAGTAAGG AGGTCAAGAA CATCCCCGAT GATCCCTGCC ACCACTTTCT TCTTGAGTTT
    GCACAGTCAG ACCCTGCCCA GAACTTCTGT GGGCCATTTT CAGAACTTTT CAAAGGATTC
    ATACAGGCAT GTAGAAAACA GGCCCCAAAG ACAGATATAG TTGCTGGTTC CACTATTGAT
    CAAGCTGTGA ACGCCACCTT TGCTGCTCTG GTGTGTCGTA CTCCAGGCTT ATATGAGAAG
    CTGCAAAAAT ATGTAAACAG TGGAGGCAAC ACAGCCCTGA GCGAGGACTT TGCACAGGTG
    TATTGCTTGG CAGATGGGAT CCGAGTATGG ATGCTGGAGA TGAAGCAGAA GTCATTGATG
    AACCTGGGGA ATGAGGCAGA AGAAAAGCAT GGCCCGGAAG CTGCCGAGGC AAACCCTGAG
    AGCCTGGCAA AAGAGTGTAT TCAGAAGAGT CTTCTGTTAC TACGATTTTT GCCCATGGGA
    GTAAGTTCAA AAGAAAGCTG TGACAAGCTG GTGGCTGCAG TAGAAACAGA TCACCTGCAG
    CCCTGCGACA GGTGCCCAAG GACGAGCTCT GTGGTGGAGG AGCATTTCCA GGCCTCGGAG
    TCTCCCATGG ACACAGGCAC CCTTGCCGCA AGCAGTGGGG GTCCTAGCTC AGATGTGCCG
    CTGAAGCTGC TGCCCAGCGG TGGCCCCCCT GCCACTGAGG TGTCCTTTGC CACTGCTGAA
    GAGCCCTCTT CACCTTCCGC TCCTGCCCGG CGGCCTCCCT TCACCCGAGG GCGACTTCGA
    CTGCTCTCCT TCCGGTCAGT GGAGGAGGCC AGACCCGTGC CCACCGTGAA GGAGAAATAC
    CCCGTGCTGA AGGATGTCCT GGACTTCATT AAGGACCAGT CCCTCTCTCA TGAGAGTGTC
    GTAAAGGTTC TTGCCTTGAG GAAAGCTCAA GCTCAGAGCA TCCTGGAGGT CCTGAGGATC
    ATTCAGTACT GCACGGAGTC TCTGGGACGG CCTCATTGCT TCCACCCGCC GTGTATACTT
    TTCCTGATGG AGCTTCTCAC CTGCCAGAAA GAGTTTACCA ATTATTTCGC ACACATGGAG
    GGCTGTGGTG CACGTCTGCG CAAAGAGATT CGAGACACTT ACTACCAGTT TGTTCTGTTT
    TTGGTTAAAG CAGTTAAAGA ATTTAGCAGC ATGAACGACA GGTCGCTGCT GCCTGCCTTG
    TCCTGTGTGC AGACAGCCCT GCTCCACCTT CTGGATATGG GCTGGGAACC CGGCGATCTC
    GCTTTCTTTG TGGACATTGG GTTACCACAC CTCCTCATGA AACTGTCACA AGAGAATATA
    AGCGTCCACG ACAGCGTGAT CAGCCAGTGC AGTGAAGAAG ATGAGCTCGC GGATGCCAAG
    CAGAATTCAG AATGGATGGA TGAGTGTCAG GATGGCATGT TTGAGGCCTG GTATGAAAAA
    ATAGCCCAGG AAGATCCAGA GAAGCAGAGG AAAATGCACA TGTTCATTGC TCGCTACTGC
    GACCTGCTAA ACGTGGACAT CTCCTGTGAT GGCTGTGATG AGATTGCCCC CTGGCACCGC
    TACCGGTGTC TGCAGTGCAG TGACATGGAT CTCTGCAAAA CTTGCTTCCT AGGTGGGGTG
    AAGCCCGAGG GCCATGGAGA TGATCATGAG ATGGTCAGCA TGGAGTTTAC CTGTGACCAC
    TGCCAGGGTT TGATCATCGG CCGGAGGATG AACTGCAACG TGTGTGATGA CTTCGATCTT
    TGCTACGGGT GCTACGCGGC AAAGAGATAC TCTTACGGCC ACTTGCCCAC CCACGGCGTC
    ACAGCCCACC CGATGGTGAC CATCCGGATC AGCGACCGGC AGAGGCTTAT CCAGCCGTAC
    ATCCACAACT ACGCCTGGCT GCTCTTTGCT GCCCTGGCTC TCTACAGCGC CCACCTGGCC
    AGTGCAGAGG ACGTGGATGG TGAGAAGCTG AATCCCCAGG ACCGTGGCAG TGCCAGCCTG
    CTGCGGAGCC AGTGCATGCA GCTTGTCGGG GACTGTCTGA TGAAGGCTCA CCAGGGAAAA
    GGTCTGAAAG CTCTAGCTCT CCTTGGTATA TTGCCAGATG GTGAACCTAG TCCAGAAAGT
    CGGGCCCCAC CCGTCACTGT GCCCACCCAC ACGGCAGAGG AGGAGAGAGC TGTCTGGGGC
    CCTGAGGACC TGCCACATAC AGGCCTTTCT ATGTGCTACA GTGGAAAGGA AGATGGTCCT
    AAGGAAGTCA GACCTTTAGA TAGCAGGCAG AGAAGTAAGG CAGATGAAGA AGGTGCATCC
    TTAATGAATG GTCCTTCATG CCAGACTCCA ATCTCAGACA CACCTGCAGA TGGTAGCACC
    CCTGCAAGGC TTCCAGATGC TGAGAATACA GTCATGTTGT CTCAGGAGTC CATCGAGGAA
    AAGGCGGTTA CTCCAAGTCC TGAGCAGGTG TTTGCTGAGT GCTCCCAGAA GAGGATTTTA
    GGATTACTAG CAGCCATGTT ACCTCCCTTA AAGTTGGACC CCACAGTCCC CGTGATAGAC
    CTGGAGCACG TCCTCCCACT CATGTTTCAG GTTGTCATCT CAAACGTAGG CCACCTGAAT
    GAGACCTACC ACCTCACGCT GGGTCTTCTC GGCCAGCTCA TCACCCGCCT CTTACCAGCG
    GAGGTAGATG CTGCGGTGGT GAAAGTCCTC TCCGCCAAGC ATACCTTGTT TGCAACAGGG
    GACAGTTCTG TGGTGCCAGA TGGCTGGAAG ACCACCCACC TGCTCTTTAG CCTGGGAGCC
    GTGTGTCTGG ACAGCCGGGT GGGCTTGGAC TGGGCATGCT CCATGGCAGA GATCCTGCGG
    TCCCTCAACA GTGCCCCACT GTGGCGTGAC GTCATCGCCG CCTTCACAGA CCACTGTATC
    AGGCAGCTGC CTTTCCAGCT GAAGCACACC AACATCTTCA CCCTGCTCGT GCTGGTCGGC
    TTCCCACAGG TCCTCTGCGT GGGGACCCGC TGTGTGTATG TGGATCATGC CAATGAGCCA
    CATAATGTCA TCATCTTGAA GCACTTCACG GAGAAGAACA GGGCTGTGAT CGTTGATGTC
    AAAACCCGGA AAAGGAAAAC AGTGAAGGAC TACCAGCTGG TCCAGAAAGG ACAGGAATGC
    AGCAGCTCTC AGGCCCAGCT GAGCCAGTAC TCCCGGCACT TTGCCTTTAT CGCCAGTCAC
    CTTCTACAGA CCAGTATGGA CAGCCAATGT CCTGAGGCAG TAGAAGCAAC ATGGGTCCTG
    TCTCTGGCCC TGAAAGGACT GTATAAAACA CTAAAGGCTC ACAGTTTTGA AGAGACCCAC
    ACCACCTTCC TTCAGACGGA TTTGCTGAAG CTGCTGGTGA AAAAGTGCAG CAAAGGGACT
    GGTTTCAGTA AAACGTGGCT CCTCCGGGAT CTGGAGATCC TGTCCATCAT GCTGTACTCC
    TCCAAGAAGG AGAGCCATAC CTCGGCTGAG CACACAGCCC TGGAGCGAGA TGAGAGAGGA
    GACCAAGAGG AGGAGGTGGA TGGCACAGTC AGCAGCCCTG GTGAGCCAGA CCAAAAGAAG
    CTAGACCCTC TGGAGAGCCT GGACGAGCCT ACCAGAATAT GTTTCTTGAT GGCGCACGAT
    GCGCTCAATG CTCCTCTGCA CATCCTCCGG GCCATCTACG AGCTGCAGAT GAAAAGGACG
    GATTCTTTCT TCCTGGAGGT TCAGAAGAGG TTTGATGGGG ATGAACTCAC CACAGATGAG
    AGGATTCGCA CCCTGGCTCA GAGATGGCAG CCCAGTAGGG GCCTGAGACT GGAGGAACAG
    AGCACCAAAG CCGTGGATAC AGACATGATC ATCTTACCGT GTTTGTCCCG GCCTGCCCGC
    TGTGACCAGG CCACGGCCGA ATCCAACCCC GTGACCCAGA AGCTGATCTC CAGCACTGAG
    AGTGAATTGC AGCATAGCTA CGCCAAACAG CGGCGCAGCA AGAGCACCGC CCTCCTGCAC
    AAGGAGCTGA ATGGCAAGAG CAAGCGGGCC ATCCGGGAGT ACCTGTTCCG CGTGAACGAA
    GCAACAGCTG TCCTGTATGC CCGCCATGTG CTGGCATCCT TGCTCGCCGA GTGGCCGGGG
    CACGTACCAG TGAGCGAGGA CACCCTGGAC CTCAGCGGCC CTGCCCACAT GACCTACATC
    TTGGACATGT TCATGCAGCT GGAGGAGAAG CACCAGTGGG AGAAGATCCT GCGGAAGGTG
    CTTCAGGGCT GCCGAGGGAG CATGCTGGGG ACCATGGCTC TGGCCGCCTG CCAGTTCATG
    GAGGAGCCGG GGATGGAGGT GCAAGTGAGA GAGTCAAAGC ACCCGTATAA CAACAACACC
    AGCTTTGAGG ATAAAGTCCA CATCCCTGGT GCAATCTACC TCTCGATCAA ATTTGACTCG
    CAGTGTAGCA CAGAGGAGGG CTGTGACGAG CTAGCCATGT CCAGCAGTAG CGACTTCCAG
    CAGGATCGAC ACACTTTCAG TGGGTCTCAG CAGAAATGGA AGGATTTTGA GCTTCCAGGG
    GATACTCTAT ACTACCGCTT CACCTCTGAC ATGAGCAACA CGGAGTGGGG TTACAAGTTC
    ACTGTGACAG CTGGACACCT GGGCCGATTC CAGACAGGCT TCGAGATTTT GAAGCAGATG
    TTGTCAGAAG AAAGAGTTGT GCCACACCTC CCACTGGCCA AAATTTGGGA GTGGCTGGTG
    GGGGTGGCTT GTCGCCAGAC TGGTCATCAG CGATTGAAAG CCATCCACCT GCTTCTGAGG
    ATTGTGCAAT GCTGCACCCA CAGCGACCTC TGTGACCTTG CGCTGCTAAA GCCCCTGTGG
    CAGCTCTTCA CTCACATGGA GTATAGCCTG TTCGAGGATG TGACCTCACC CGGCATCCTC
    CTCCCCCTGC ACCGTGCCCT CACTGAGCTC TTCTTCGTCA CCGAGAACCG CGCCCAGGAG
    CTCGGCTTGC TGCAGGAGTA TCTGCTGGCC TTGACCACGG ACGACCACCT TCTCCGCTGC
    GCGGCACAGG CCCTGCAGAA TGTCGCTGCT ATCAGCCTGG CCATCAACTA CCCAAACAAG
    GCAACCCGCC TCTGGAACGT TGAGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274103.1
    CDS68..8557
    Translation

    Target ORF information:

    RefSeq Version XM_022418395.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris zinc finger ZZ-type and EF-hand domain containing 1 (ZZEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418395.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGCTGGAGA CCCTGAAGAA TTCCAGTGGA GCCAATCTCC AGGGGGAGCT GAGCCACATC 
    ATCAGACAGC TGCAGGCCTG TTCTCTTGTC CCAGGTTTCA TAGACATATT CTCAGAGTCC
    AAGGAGGGTC TGGGTGTGCA CGCATCCTTG ATCCTGCGTT TCCTGCACCG CAATCGGCTC
    TCCGGCATGG CCATCCCCTG CCCCATGCTG GACCACTGCA GTAACATGTG CTCCATGAGG
    GCTTCTGTTC TGAAGGAGTC TTTGGACCAG CTGGTACAGA GAGAAAAGGA AAGTCCTGGA
    GATCTAACCA GAAGCCCAGA GATGGATAAA CTCAAGTCAG TAGCAAAGTG CTACGCTTAC
    ATAGAAACAT CCTCCAACCC TGCAGACATC GACAAGATGA CAAATGGAGA AACATCATCA
    TACTGGCAAT CAGATGGCAG TGCTCGCTCA CACTGGATTC GCTTAAAGAT GAAGCCAGAT
    GTTGTGCTTA GGCACTTGTC CATTGCTGTG GCTGCCACTG ACCAGAGCTA CATGCCGCAG
    CAGGTGACGG TCGCTGTGGG CAGAAGTGCC AGCGATCTTC AGGATGTCCG CGATGTGCAC
    ATTCCCAGCA ATGTCACTGG CTATGTGACA CTTCTGGAGA ACGCCAACAT TAGTCAGCTG
    TATGTCCAGA TTAACATAAA ACGTTGTCTT AGCGATGGAT GTGACACTAG AATTCATGGT
    CTGAGGGCTG TTGGCTTTCA GAGAGTTAAG AAGTCTGGGG TCTCCGTGTC AGATGCTTCT
    GCAATCTGGT ACTGGTCTCT GCTGACCTCT CTGGTGACGG CGTCCATGGA GACCAACCCT
    GCCTTCGTCC AGACAGTGCT GCACAACACG CAGAAGGCAC TACAGCACAT GCCTCCACTT
    TCCCTCTCAC CAGGATCTAC AGATGTCTCA ACTTTCCTGT CTCCCAACGT TCTAGAAGAG
    GTGGACAGCT TCCTCATAAG GATAACTAGC TGCTGTTCAA CTCCAGAGGT GGAGCTGACT
    CTTCTGGCCT TTGCACTAGC AAGAGGAAGT GTTGCCAAAG TGCTGAGCTG TCTGTGTACC
    ATTGCTGACC ACCTGGACAC ACGCTATGAT GCCTCGTCCC TCATCTCCTC CATGGCATCA
    GTCAGGCAGA CCCTGCTCCT TAAATACGGT AAACCTCTTC AGCTGACCCT TCAGGCCTGT
    GATGTTAAAG GGAAAGAAGA GAAATCAGGG CCCGAAAACC TCCTTGTCGA GCCGTGGACA
    AGAGATGGGT TTCTTACAGA GACGGGAAAG ACCAGAGCAA GTACCATTTT TTCTGCAGGA
    ACTGAATCTG CCTTCCAAGT TACACAGATC AGGATTATGG TTCGACGTGG TGGCATCGGT
    GCCCAATGTG GGTTGGTGTT TGCCTATAAC TCATCTTCTG AAAAATTTCA TGCCGAAGAA
    CACTTCAAAA GGTTTGAAAA ATATGACAAG TGGAAGCTTC AGGAATTCAG GCAATTTGTA
    AAGAGCAGGG TTGGTTGCTC AGCTGATGAC CTTGGCGAGG ATGACCCTAT TGGCTGGTTT
    GAGCTAGAAG AAGAATGGGA TGAAGCAGAT GTCAAGTTGC AGCAGTGCAG AGTTGCCAAA
    TATTTGATGG TGAAGTTCCT CTGCACCCGT CAGGAGTCGG CAGAGCGCTT GGGAGTGCAA
    GGTCTCAGCA TCAGCGGGTA TGTCCGGCCA ATGCGAGTGG AAGCGGAGCA GCACATTGTC
    TGTGCCCACT GCAGAAAGGA TGGGGAGGAG AGTGTCTGCG GGACCACGCT ACTCCTCAGG
    ACGCTTCAGT TCACTCAGCA GCTTGCCCAT GACCTGGTGC AGCAGAAGGA AAGTGGCTTA
    AAATATAAAT CTTTTCTGGA CTTCGCGGGT CTTGATTTGC AGATCTTCTG GAATTTTTAT
    AGTAAATTAA AGCAAAACCC GAGGGAAGAA TGCATCTGTG CCCAGACCCT GCTTCTGCGG
    CTCCTGCAGA GCTGCTTGTC AGTGCTGCAG AGAGACGGGC CAGCCACCCC CGAGGAGGCC
    AAGGCTCCCC GGCAGAGCCC TGGAGGGGCG GGGGCTGCCA AGGAACTCCA CGCACACTTA
    TGCGACGTGG TGGATAGGGT GGACGGAGAC TCTGTGCCCA TGGAAACATT AAAACAAGAA
    GTCAGGAACA CCCTTCTCAG TGGGGCTGCC GTCTTTTTTC CTGATCGACA GAGCCGGAGG
    AGCCATCTCT TCACCATGAT GAAGAATGTC ACCGAGCAGG AACACAAGCA GTCCCAGCAG
    CTCACTTTCC AGTCACTATG CACCTATTTT AGTGACAAGG ATCCAGGTGG TCTTCTGCTC
    TTGCCTGAGA AAGGTGACCT GGCTGACATG AACAGCAGCG AAGTTCTGGC CGTCATGAGC
    ACCCTCCTGT CCGTTGCTGC TCGAGAGTGT GAGCTCCTGC TGCTTACCAG GGCCCAGGGA
    CAGCCCGGCT CCGTGCTCTT CTCCCTGTTC TGGGCCGTCC AGGGCAGCCT GCTCTCCTGG
    TGCTACCTGC AGCTGAAGAG CACCGACGCG GGAGCCCGGG GTCTCGCCGC CCACCTCATT
    GACCAGTATG TGGGACAGTT TGTGGCAAGC ACGAGAGTGA TTCTGGAATC CCTTTTGTCA
    CAGTATAGTG GACAAACCAT TGTAGAGAAA CTGTGTAATT CAGTGTTTTC GATGGCAGCT
    CGTCAACTGG TGATCTTCCT GCTGGACTTT TGCACTTTAG ACATTTCACA CTGTGCACTC
    CTGAGAGAGT TCAGTACACT GACAGAACTG TTAAAGCAGA TCTGCGCGGA CCCCACAGGA
    GCGCCAGACA AGCTCGATGA AGACACTTGG CAGCAGGAGT GTCCTGTGGT ATTACACACC
    TGGACCAAGG AGTCTGCCCA CAACTATGAG AACAATTGCC ATGAGGTGTC TGTCTTCATC
    AGCCCTGGGG CAACCTACTT TGAGGTAGAA TTTGATGAGC GCTGTGAGAC TGAGAAGAGG
    TACGATTATT TGGAGTTCAC TGACGCTAGA GGCGGGAAAA CACGCTATGA CACCAAAGTT
    GGCACTGAGA AGTGGCCCAG GAAAGTGATC TTCAAGGCTG GCCCCCGGCT GCAGTTCCTC
    TTTCACTCTG ACAGCAGTAA CAATGAGTGG GGTTACAAAT TCACCGTCAC CGCCTATGGC
    CTGCCCGATG TGGCCGTGTC CTGGGCCCTG GATTTACAGC TGCTCGTTTG CCGGCTGATG
    GGACGCCTCG CTGCCCAGTG CATGGCGCTC AAGTCTGCGC ACCAGTTTGG AGGCGACATG
    GCAGTGGCTC CAGCAAAAAT GGCGTTGGTC CTGAACTCTC CGCTGTGGAA ACCTGTTTTC
    AGGCATCAGT TGAGTCCAGA GTTGGGATTA GCAGTGAGCT GGCCCACGCA CCTGCACCAG
    GATAGTAAGG AGGTCAAGAA CATCCCCGAT GATCCCTGCC ACCACTTTCT TCTTGAGTTT
    GCACAGTCAG ACCCTGCCCA GAACTTCTGT GGGCCATTTT CAGAACTTTT CAAAGGATTC
    ATACAGGCAT GTAGAAAACA GGCCCCAAAG ACAGATATAG TTGCTGGTTC CACTATTGAT
    CAAGCTGTGA ACGCCACCTT TGCTGCTCTG GTGTGTCGTA CTCCAGGCTT ATATGAGAAG
    CTGCAAAAAT ATGTAAACAG TGGAGGCAAC ACAGCCCTGA GCGAGGACTT TGCACAGGTG
    TATTGCTTGG CAGATGGGAT CCGAGTATGG ATGCTGGAGA TGAAGCAGAA GTCATTGATG
    AACCTGGGGA ATGAGGCAGA AGAAAAGCAT GGCCCGGAAG CTGCCGAGGC AAACCCTGAG
    AGCCTGGCAA AAGAGTGTAT TCAGAAGAGT CTTCTGTTAC TACGATTTTT GCCCATGGGA
    GTAAGTTCAA AAGAAAGCTG TGACAAGCTG GTGGCTGCAG TAGAAACAGA TCACCTGCAG
    CCCTGCGACA GGTGCCCAAG GACGAGCTCT GTGGTGGAGG AGCATTTCCA GGCCTCGGAG
    TCTCCCATGG ACACAGGCAC CCTTGCCGCA AGCAGTGGGG GTCCTAGCTC AGATGTGCCG
    CTGAAGCTGC TGCCCAGCGG TGGCCCCCCT GCCACTGAGG TGTCCTTTGC CACTGCTGAA
    GAGCCCTCTT CACCTTCCGC TCCTGCCCGG CGGCCTCCCT TCACCCGAGG GCGACTTCGA
    CTGCTCTCCT TCCGGTCAGT GGAGGAGGCC AGACCCGTGC CCACCGTGAA GGAGAAATAC
    CCCGTGCTGA AGGATGTCCT GGACTTCATT AAGGACCAGT CCCTCTCTCA TGAGAGTGTC
    GTAAAGGTTC TTGCCTTGAG GAAAGCTCAA GCTCAGAGCA TCCTGGAGGT CCTGAGGATC
    ATTCAGTACT GCACGGAGTC TCTGGGACGG CCTCATTGCT TCCACCCGCC GTGTATACTT
    TTCCTGATGG AGCTTCTCAC CTGCCAGAAA GAGTTTACCA ATTATTTCGC ACACATGGAG
    GGCTGTGGTG CACGTCTGCG CAAAGAGATT CGAGACACTT ACTACCAGTT TGTTCTGTTT
    TTGGTTAAAG CAGTTAAAGA ATTTAGCAGC ATGAACGACA GGTCGCTGCT GCCTGCCTTG
    TCCTGTGTGC AGACAGCCCT GCTCCACCTT CTGGATATGG GCTGGGAACC CGGCGATCTC
    GCTTTCTTTG TGGACATTGG GTTACCACAC CTCCTCATGA AACTGTCACA AGAGAATATA
    AGCGTCCACG ACAGCGTGAT CAGCCAGTGC AGTGAAGAAG ATGAGCTCGC GGATGCCAAG
    CAGAATTCAG AATGGATGGA TGAGTGTCAG GATGGCATGT TTGAGGCCTG GTATGAAAAA
    ATAGCCCAGG AAGATCCAGA GAAGCAGAGG AAAATGCACA TGTTCATTGC TCGCTACTGC
    GACCTGCTAA ACGTGGACAT CTCCTGTGAT GGCTGTGATG AGATTGCCCC CTGGCACCGC
    TACCGGTGTC TGCAGTGCAG TGACATGGAT CTCTGCAAAA CTTGCTTCCT AGGTGGGGTG
    AAGCCCGAGG GCCATGGAGA TGATCATGAG ATGGTCAGCA TGGAGTTTAC CTGTGACCAC
    TGCCAGGGTT TGATCATCGG CCGGAGGATG AACTGCAACG TGTGTGATGA CTTCGATCTT
    TGCTACGGGT GCTACGCGGC AAAGAGATAC TCTTACGGCC ACTTGCCCAC CCACGGCGTC
    ACAGCCCACC CGATGGTGAC CATCCGGATC AGCGACCGGC AGAGGCTTAT CCAGCCGTAC
    ATCCACAACT ACGCCTGGCT GCTCTTTGCT GCCCTGGCTC TCTACAGCGC CCACCTGGCC
    AGTGCAGAGG ACGTGGATGG TGAGAAGCTG AATCCCCAGG ACCGTGGCAG TGCCAGCCTG
    CTGCGGAGCC AGTGCATGCA GCTTGTCGGG GACTGTCTGA TGAAGGCTCA CCAGGGAAAA
    GGTCTGAAAG CTCTAGCTCT CCTTGGTATA TTGCCAGATG GTGAACCTAG TCCAGAAAGT
    CGGGCCCCAC CCGTCACTGT GCCCACCCAC ACGGCAGAGG AGGAGAGAGC TGTCTGGGGC
    CCTGAGGACC TGCCACATAC AGGCCTTTCT ATGTGCTACA GTGGAAAGGA AGATGGTCCT
    AAGGAAGTCA GACCTTTAGA TAGCAGGCAG AGAAGTAAGG CAGATGAAGA AGGTGCATCC
    TTAATGAATG GTCCTTCATG CCAGACTCCA ATCTCAGACA CACCTGCAGA TGGTAGCACC
    CCTGCAAGGC TTCCAGATGC TGAGAATACA GTCATGTTGT CTCAGGAGTC CATCGAGGAA
    AAGGCGGTTA CTCCAAGTCC TGAGCAGGTG TTTGCTGAGT GCTCCCAGAA GAGGATTTTA
    GGATTACTAG CAGCCATGTT ACCTCCCTTA AAGTTGGACC CCACAGTCCC CGTGATAGAC
    CTGGAGCACG TCCTCCCACT CATGTTTCAG GTTGTCATCT CAAACGTAGG CCACCTGAAT
    GAGACCTACC ACCTCACGCT GGGTCTTCTC GGCCAGCTCA TCACCCGCCT CTTACCAGCG
    GAGGTAGATG CTGCGGTGGT GAAAGTCCTC TCCGCCAAGC ATACCTTGTT TGCAACAGGG
    GACAGTTCTG TGGTGCCAGA TGGCTGGAAG ACCACCCACC TGCTCTTTAG CCTGGGAGCC
    GTGTGTCTGG ACAGCCGGGT GGGCTTGGAC TGGGCATGCT CCATGGCAGA GATCCTGCGG
    TCCCTCAACA GTGCCCCACT GTGGCGTGAC GTCATCGCCG CCTTCACAGA CCACTGTATC
    AGGCAGCTGC CTTTCCAGCT GAAGCACACC AACATCTTCA CCCTGCTCGT GCTGGTCGGC
    TTCCCACAGG TCCTCTGCGT GGGGACCCGC TGTGTGTATG TGGATCATGC CAATGAGCCA
    CATAATGTCA TCATCTTGAA GCACTTCACG GAGAAGAACA GGGCTGTGAT CGTTGATGTC
    AAAACCCGGA AAAGGAAAAC AGTGAAGGAC TACCAGCTGG TCCAGAAAGG ACAGGAATGC
    AGCAGCTCTC AGGCCCAGCT GAGCCAGTAC TCCCGGCACT TTGCCTTTAT CGCCAGTCAC
    CTTCTACAGA CCAGTATGGA CAGCCAATGT CCTGAGGCAG TAGAAGCAAC ATGGGTCCTG
    TCTCTGGCCC TGAAAGGACT GTATAAAACA CTAAAGGCTC ACAGTTTTGA AGAGACCCAC
    ACCACCTTCC TTCAGACGGA TTTGCTGAAG CTGCTGGTGA AAAAGTGCAG CAAAGGGACT
    GGTTTCAGTA AAACGTGGCT CCTCCGGGAT CTGGAGATCC TGTCCATCAT GCTGTACTCC
    TCCAAGAAGG AGAGCCATAC CTCGGCTGAG CACACAGCCC TGGAGCGAGA TGAGAGAGGA
    GACCAAGAGG AGGAGGTGGA TGGCACAGTC AGCAGCCCTG GTGAGCCAGA CCAAAAGAAG
    CTAGACCCTC TGGAGAGCCT GGACGAGCCT ACCAGAATAT GTTTCTTGAT GGCGCACGAT
    GCGCTCAATG CTCCTCTGCA CATCCTCCGG GCCATCTACG AGCTGCAGAT GAAAAGGACG
    GATTCTTTCT TCCTGGAGGT TCAGAAGAGG TTTGATGGGG ATGAACTCAC CACAGATGAG
    AGGATTCGCA CCCTGGCTCA GAGATGGCAG CCCAGTAGGG GCCTGAGACT GGAGGAACAG
    AGCACCAAAG CCGTGGATAC AGACATGATC ATCTTACCGT GTTTGTCCCG GCCTGCCCGC
    TGTGACCAGG CCACGGCCGA ATCCAACCCC GTGACCCAGA AGCTGATCTC CAGCACTGAG
    AGTGAATTGC AGCATAGCTA CGCCAAACAG CGGCGCAGCA AGAGCACCGC CCTCCTGCAC
    AAGGAGCTGA ATGGCAAGAG CAAGCGGGCC ATCCGGGAGT ACCTGTTCCG CGTGAACGAA
    GCAACAGCTG TCCTGTATGC CCGCCATGTG CTGGCATCCT TGCTCGCCGA GTGGCCGGGG
    CACGTACCAG TGAGCGAGGA CACCCTGGAC CTCAGCGGCC CTGCCCACAT GACCTACATC
    TTGGACATGT TCATGCAGCT GGAGGAGAAG CACCAGTGGG AGAAGATCCT GCGGAAGGTG
    CTTCAGGGCT GCCGAGGGAG CATGCTGGGG ACCATGGCTC TGGCCGCCTG CCAGTTCATG
    GAGGAGCCGG GGATGGAGGT GCAAGTGAGA GAGTCAAAGC ACCCGTATAA CAACAACACC
    AGCTTTGAGG ATAAAGTCCA CATCCCTGGT GCAATCTACC TCTCGATCAA ATTTGACTCG
    CAGTGTAGCA CAGAGGAGGG CTGTGACGAG CTAGCCATGT CCAGCAGTAG CGACTTCCAG
    CAGGATCGAC ACACTTTCAG TGGGTCTCAG CAGAAATGGA AGGATTTTGA GCTTCCAGGG
    GATACTCTAT ACTACCGCTT CACCTCTGAC ATGAGCAACA CGGAGTGGGG TTACAAGTTC
    ACTGTGACAG CTGGACACCT GGGCCGATTC CAGACAGGCT TCGAGATTTT GAAGCAGATG
    TTGTCAGAAG AAAGAGTTGT GCCACACCTC CCACTGGCCA AAATTTGGGA GTGGCTGGTG
    GGGGTGGCTT GTCGCCAGAC TGGTCATCAG CGATTGAAAG CCATCCACCT GCTTCTGAGG
    ATTGTGCAAT GCTGCACCCA CAGCGACCTC TGTGACCTTG CGCTGCTAAA GCCCCTGTGG
    CAGCTCTTCA CTCACATGGA GTATAGCCTG TTCGAGGATG TGACCTCACC CGGCATCCTC
    CTCCCCCTGC ACCGTGCCCT CACTGAGCTC TTCTTCGTCA CCGAGAACCG CGCCCAGGAG
    CTCGGCTTGC TGCAGGAGTA TCTGCTGGCC TTGACCACGG ACGACCACCT TCTCCGCTGC
    GCGGCACAGG CCCTGCAGAA TGTCGCTGCT ATCAGCCTGG CCATCAACTA CCCAAACAAG
    GCAACCCGCC TCTGGAACGT TGAGTGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.