BACE1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	BACE1
Entrez Gene ID	489390
Full Name	beta-secretase 1
Synonyms	ASP2
General protein information	Preferred Names beta-secretase 1 Names beta-secretase 1 aspartyl protease 2 beta-site APP-cleaving enzyme 1 beta-site amyloid precursor protein cleaving enzyme 1 membrane-associated aspartic protease 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001284472.1	NP_001271401.1	beta-secretase 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	BACE1	NP_036236.1
Gallus gallus (chicken)	BACE1	XP_417908.3
Danio rerio (zebrafish)	bace1	NP_991267.1
Xenopus tropicalis (tropical clawed frog)	bace1	NP_001072485.1
Bos taurus (cattle)	BACE1	NP_001039996.1
Mus musculus (house mouse)	Bace1	NP_035922.4
Rattus norvegicus (Norway rat)	Bace1	NP_062077.1
Pan troglodytes (chimpanzee)	BACE1	XP_001158428.1
	BACE1	XP_001094263.1
Canis lupus familiaris (dog)	BACE1	XP_546508.3

Gene Ontology
Bibliography

Related articles in PubMed

Cloning, sequencing and expression in the dog of the main amyloid precursor protein isoforms and some of the enzymes related with their processing.
Sarasa L, Gallego C, Monle?n I, Olvera A, Canudas J, Monta??s M, Pesini P, Sarasa M
Neuroscience171(4)1091-101(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following BACE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BACE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34134	NM_001284472.1 Latest version!	Canis lupus familiaris beta-secretase 1 (BACE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BACE1 ( NM_001284472.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001271401.1 Canis lupus familiaris beta-secretase 1 (BACE1), mRNA.

Price & Availability↑

CloneID	OCe34134
Clone ID Related Accession (Same CDS sequence)	NM_001284472.1
Accession Version	NM_001284472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	beta-secretase 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB793314.1. ##Evidence-Data-START## Transcript exon combination :: AB793314.1, AB793312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCAAG CCCTGCCCTG GCTCCTGCTG TGGATGGGCT CCGGAGTGCT GCCCGCCCGC 
TGCACCCCGC CCGGCATCCG GCTGCCCCGG GAGACCGTCG AGGAGCCCGA CGAGCCGGGC 
CGGAGGGGCA GCTTTGTGGA GATGGTGGAC AACCTGAGAG GCAAGTCCGG TCAGGGCTAC 
TACGTGGAGA TGACCGTGGG CAGCCCCCCG CAGACGCTCA ACATCCTGGT GGACACAGGC 
AGCAGTAACT TTGCAGTGGG GGCTGCCCCC CACCCTTTCC TGCATCGCTA CTACCAGAGG 
CAGCTGTCCA GTACATACCG GGACCTCCGA AAGGGCGTGT ATGTGCCCTA CACCCAGGGC 
AAGTGGGAGG GGGAGCTGGG CACCGACCTG CTCTGTGGCG CTGGCTTCCC CCTCAACCAG 
TCAGAAGTGC TGGCCTCGGT TGGAGGGAGC ATGATCATCG GGGGTATCGA CCACTCACTG 
TACACGGGCA GCCTCTGGTA CACACCCATC CGGCGGGAGT GGTACTACGA GGTGATCATT 
GTAAGGGTGG AGATCAACGG GCAGGACCTG AAAATGGACT GCAAGGAGTA CAACTATGAC 
AAGAGCATTG TGGACAGTGG CACCACCAAC CTTCGTTTGC CCAAGAAAGT GTTTGAAGCT 
GCTGTCAAAT CCATCAAGGC AGCCTCTTCG ACGGAGAAGT TCCCGGATGG GTTCTGGCTG 
GGGGAGCAGC TGGTGTGCTG GCAGGCAGGC ACCACCCCTT GGAACATTTT CCCGGTCATC 
TCCCTCTACC TGATGGGCGA GGTCACCAAT CAGTCCTTCC GCATTACCAT CCTTCCTCAG 
CAATACCTGC GGCCAGTGGA AGACGTGGCC ACATCCCAAG ACGACTGCTA CAAATTCGCC 
ATTTCACAGT CATCCACGGG CACCGTAATG GGAGCTGTTA TCATGGAGGG CTTCTACGTT 
GTCTTTGACC GGGCCCGAAA ACGAATTGGC TTTGCCGTCA GTGCTTGTCA TGTGCACGAT 
GAGTTCAGGA CGGCAGCAGT GGAAGGCCCG TTTATCACCC TGGACATGGA AGACTGTGGC 
TACAACATTC CACAGACAGA TGAGTCAACC CTCATGACCA TAGCCTACGT CATGGCTGCC 
ATTTGCGCCC TCTTCATGCT GCCGCTCTGC CTCATGGTGT GCCAGTGGCG CTGCCTCCGC 
TGCCTGCGCC ATCAACATGA TGACTTTGCC GATGACATCT CCCTGCTGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271401.1
CDS	1..1254
Translation	MAQALPWLLLWMGSGVLPARCTPPGIRLPRETVEEPDEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFITLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRHQHDDFADDISLLK

Target ORF information:

RefSeq Version	NM_001284472.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris beta-secretase 1 (BACE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284472.1

ORF Insert Sequence:

ATGGCCCAAG CCCTGCCCTG GCTCCTGCTG TGGATGGGCT CCGGAGTGCT GCCCGCCCGC 
TGCACCCCGC CCGGCATCCG GCTGCCCCGG GAGACCGTCG AGGAGCCCGA CGAGCCGGGC 
CGGAGGGGCA GCTTTGTGGA GATGGTGGAC AACCTGAGAG GCAAGTCCGG TCAGGGCTAC 
TACGTGGAGA TGACCGTGGG CAGCCCCCCG CAGACGCTCA ACATCCTGGT GGACACAGGC 
AGCAGTAACT TTGCAGTGGG GGCTGCCCCC CACCCTTTCC TGCATCGCTA CTACCAGAGG 
CAGCTGTCCA GTACATACCG GGACCTCCGA AAGGGCGTGT ATGTGCCCTA CACCCAGGGC 
AAGTGGGAGG GGGAGCTGGG CACCGACCTG CTCTGTGGCG CTGGCTTCCC CCTCAACCAG 
TCAGAAGTGC TGGCCTCGGT TGGAGGGAGC ATGATCATCG GGGGTATCGA CCACTCACTG 
TACACGGGCA GCCTCTGGTA CACACCCATC CGGCGGGAGT GGTACTACGA GGTGATCATT 
GTAAGGGTGG AGATCAACGG GCAGGACCTG AAAATGGACT GCAAGGAGTA CAACTATGAC 
AAGAGCATTG TGGACAGTGG CACCACCAAC CTTCGTTTGC CCAAGAAAGT GTTTGAAGCT 
GCTGTCAAAT CCATCAAGGC AGCCTCTTCG ACGGAGAAGT TCCCGGATGG GTTCTGGCTG 
GGGGAGCAGC TGGTGTGCTG GCAGGCAGGC ACCACCCCTT GGAACATTTT CCCGGTCATC 
TCCCTCTACC TGATGGGCGA GGTCACCAAT CAGTCCTTCC GCATTACCAT CCTTCCTCAG 
CAATACCTGC GGCCAGTGGA AGACGTGGCC ACATCCCAAG ACGACTGCTA CAAATTCGCC 
ATTTCACAGT CATCCACGGG CACCGTAATG GGAGCTGTTA TCATGGAGGG CTTCTACGTT 
GTCTTTGACC GGGCCCGAAA ACGAATTGGC TTTGCCGTCA GTGCTTGTCA TGTGCACGAT 
GAGTTCAGGA CGGCAGCAGT GGAAGGCCCG TTTATCACCC TGGACATGGA AGACTGTGGC 
TACAACATTC CACAGACAGA TGAGTCAACC CTCATGACCA TAGCCTACGT CATGGCTGCC 
ATTTGCGCCC TCTTCATGCT GCCGCTCTGC CTCATGGTGT GCCAGTGGCG CTGCCTCCGC 
TGCCTGCGCC ATCAACATGA TGACTTTGCC GATGACATCT CCCTGCTGAA GTGA