PHLDB1 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022418227.1	XP_022273935.1	pleckstrin homology-like domain family B member 1 isoform X3
XM_005619708.3	XP_005619765.1	pleckstrin homology-like domain family B member 1 isoform X15
XM_022418238.1	XP_022273946.1	pleckstrin homology-like domain family B member 1 isoform X16
XM_014113376.1	XP_013968851.1	pleckstrin homology-like domain family B member 1 isoform X7
XM_014113377.1	XP_013968852.1	pleckstrin homology-like domain family B member 1 isoform X11
XM_005619704.2	XP_005619761.1	pleckstrin homology-like domain family B member 1 isoform X6
XM_005619703.2	XP_005619760.1	pleckstrin homology-like domain family B member 1 isoform X5
XM_005619706.2	XP_005619763.1	pleckstrin homology-like domain family B member 1 isoform X9
XM_855372.4	XP_860465.3	pleckstrin homology-like domain family B member 1 isoform X14
XM_022418226.1	XP_022273934.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_022418233.1	XP_022273941.1	pleckstrin homology-like domain family B member 1 isoform X9
XM_022418237.1	XP_022273945.1	pleckstrin homology-like domain family B member 1 isoform X13
XM_005619707.2	XP_005619764.1	pleckstrin homology-like domain family B member 1 isoform X10
XM_546499.4	XP_546499.3	pleckstrin homology-like domain family B member 1 isoform X12
XM_005619699.2	XP_005619756.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_005619701.2	XP_005619758.1	pleckstrin homology-like domain family B member 1 isoform X3
XM_005619705.2	XP_005619762.1	pleckstrin homology-like domain family B member 1 isoform X8
XM_022418232.1	XP_022273940.1	pleckstrin homology-like domain family B member 1 isoform X8
XM_022418235.1	XP_022273943.1	pleckstrin homology-like domain family B member 1 isoform X11
XM_546499.5	XP_546499.3	pleckstrin homology-like domain family B member 1 isoform X14
XM_855372.5	XP_860465.3	pleckstrin homology-like domain family B member 1 isoform X17
XM_005619700.2	XP_005619757.1	pleckstrin homology-like domain family B member 1 isoform X2
XM_005619702.2	XP_005619759.1	pleckstrin homology-like domain family B member 1 isoform X4
XM_022418228.1	XP_022273936.1	pleckstrin homology-like domain family B member 1 isoform X4
XM_022418230.1	XP_022273938.1	pleckstrin homology-like domain family B member 1 isoform X6
XM_022418234.1	XP_022273942.1	pleckstrin homology-like domain family B member 1 isoform X10
XM_005619708.2	XP_005619765.1	pleckstrin homology-like domain family B member 1 isoform X13
XM_022418225.1	XP_022273933.1	pleckstrin homology-like domain family B member 1 isoform X1
XM_022418229.1	XP_022273937.1	pleckstrin homology-like domain family B member 1 isoform X5
XM_022418231.1	XP_022273939.1	pleckstrin homology-like domain family B member 1 isoform X7
XM_022418236.1	XP_022273944.1	pleckstrin homology-like domain family B member 1 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe287945	XM_022418237.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe287946	XM_022418238.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe05896	XM_005619707.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe05899	XM_855372.5 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe05888	XM_005619699.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05889	XM_005619700.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05890	XM_005619701.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05891	XM_005619702.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05892	XM_005619703.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05893	XM_005619704.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05894	XM_005619705.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe05895	XM_005619706.2 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe05897	XM_546499.5 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe05898	XM_005619708.3 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe287933	XM_022418225.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287934	XM_022418226.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287935	XM_022418227.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287936	XM_022418228.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287937	XM_022418229.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287938	XM_022418230.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287939	XM_022418231.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287940	XM_022418232.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287941	XM_022418233.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287942	XM_022418234.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe287943	XM_022418235.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe287944	XM_022418236.1 Latest version!	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe36310	XM_014113376.1 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe36311	XM_014113377.1 Latest version!	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OCe05897	XM_546499.4	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe05899	XM_855372.4	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe05898	XM_005619708.2	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
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PHLDB1 ( XM_022418237.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273945.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe287945
Clone ID Related Accession (Same CDS sequence)	XM_022418237.1
Accession Version	XM_022418237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GTTTACCGCT CCAAGATGGA TGGTGAGGCC ACCAGCCCCC TGCCCCGGAC CCGCAGTGGC 
CCCCTCCCCT CCTCCTCTGG TTCTTCTTCC TCTTCTTCGC AGCTCAGCGT GGCTACTCTG 
GGCCGGAGCC CCTCCCCAAA GAGTGCCCTA CTTGCCCAGA ATGGCACAGG CAGCCTTCCT 
CGCAACCTGG CGGCCACGCT GCAGGACATT GAGACCAAGC GCCAGCTGGC CCTGCAGCAG 
AAGGGCGAGT CGCTTCCTGC AGAGCCCCCC CCAGCAGACG ACCCAGCAGG ACAGCAGGTG 
ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG 
TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC 
CCGCTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG 
GGCGAGCACG CCTACGACAC CCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC 
TCCACAGGCG GCAACTCAGC CTGCTCGCCC GACAACATGT CCAGTGCGAG CGGCCTGGAT 
GTGGGCAAAA TCGAGGAGAT GGAGAAGATG CTGAAAGAGG CACACGCCGA GAAGAGCCGG 
CTCATGGAGT CCAGGGAGCG GGAGATGGAG CTCCGGCGGC AGGCCCTGGA GGAGGAACGG 
AGGAGGCGAG AACAGGTGGA ACGGAGGCTA CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG 
GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA CAGTTTTCCC AGGCACGACC CCTGACCCGC 
TACCTGCCAA TCCGGAAGGA GGACTTTGAC CTGAAGACCC ACATCGAGTC GTCGGGCCAC 
GGTGTCGATA CCTGCCTGCA CGTGGTGCTT AGCAGCAAGG TCTGCCGCGG CTACTTGGTC 
AAGATGGGCG GCAAGATTAA ATCATGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG 
CGCACCCTTT CCTATTATGT GGACAAGCAC GAGACGAAGC TGAAGGGGGT CATCTACTTC 
CAGGCCATCG AGGAAGTGTA CTACGATCAC CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC 
AGCTTTTCTG TGGTGACTGA GAGCCCGAAC CCAGCCCTCA CCTTCTGTGT GAAGACCCAC 
GACCGGCTGT ATTACATGGT GGCCCCGTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC 
ATCGTCACGG GAGCGGAGGG CTACACTCAG TTCATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273945.1
CDS	89..4450
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418237.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418237.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GTTTACCGCT CCAAGATGGA TGGTGAGGCC ACCAGCCCCC TGCCCCGGAC CCGCAGTGGC 
CCCCTCCCCT CCTCCTCTGG TTCTTCTTCC TCTTCTTCGC AGCTCAGCGT GGCTACTCTG 
GGCCGGAGCC CCTCCCCAAA GAGTGCCCTA CTTGCCCAGA ATGGCACAGG CAGCCTTCCT 
CGCAACCTGG CGGCCACGCT GCAGGACATT GAGACCAAGC GCCAGCTGGC CCTGCAGCAG 
AAGGGCGAGT CGCTTCCTGC AGAGCCCCCC CCAGCAGACG ACCCAGCAGG ACAGCAGGTG 
ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG 
TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC 
CCGCTCATGC ACCACTCCAT CCTGCACCAC CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG 
GGCGAGCACG CCTACGACAC CCTGAGCCTG GAGAGCTCCG ACAGCATGGA GACCAGCATC 
TCCACAGGCG GCAACTCAGC CTGCTCGCCC GACAACATGT CCAGTGCGAG CGGCCTGGAT 
GTGGGCAAAA TCGAGGAGAT GGAGAAGATG CTGAAAGAGG CACACGCCGA GAAGAGCCGG 
CTCATGGAGT CCAGGGAGCG GGAGATGGAG CTCCGGCGGC AGGCCCTGGA GGAGGAACGG 
AGGAGGCGAG AACAGGTGGA ACGGAGGCTA CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG 
GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA CAGTTTTCCC AGGCACGACC CCTGACCCGC 
TACCTGCCAA TCCGGAAGGA GGACTTTGAC CTGAAGACCC ACATCGAGTC GTCGGGCCAC 
GGTGTCGATA CCTGCCTGCA CGTGGTGCTT AGCAGCAAGG TCTGCCGCGG CTACTTGGTC 
AAGATGGGCG GCAAGATTAA ATCATGGAAG AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG 
CGCACCCTTT CCTATTATGT GGACAAGCAC GAGACGAAGC TGAAGGGGGT CATCTACTTC 
CAGGCCATCG AGGAAGTGTA CTACGATCAC CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC 
AGCTTTTCTG TGGTGACTGA GAGCCCGAAC CCAGCCCTCA CCTTCTGTGT GAAGACCCAC 
GACCGGCTGT ATTACATGGT GGCCCCGTCT GCAGAGGCCA TGCGCATCTG GATGGACGTC 
ATCGTCACGG GAGCGGAGGG CTACACTCAG TTCATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418238.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273946.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.

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CloneID	OCe287946
Clone ID Related Accession (Same CDS sequence)	XM_022418238.1
Accession Version	XM_022418238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC 
CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG 
AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC 
CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC 
GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA GACGACCCAG CAGGACAGCA GGTGATCGAG 
GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG 
TGGGACGCCC TGCACGGGGC GGCCCCCTTC CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC 
ATGCACCACT CCATCCTGCA CCACCTGCCG GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG 
CACGCCTACG ACACCCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACA 
GGCGGCAACT CAGCCTGCTC GCCCGACAAC ATGTCCAGTG CGAGCGGCCT GGATGTGGGC 
AAAATCGAGG AGATGGAGAA GATGCTGAAA GAGGCACACG CCGAGAAGAG CCGGCTCATG 
GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG 
CGAGAACAGG TGGAACGGAG GCTACAGAGC GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG 
AAGGAGGTCA AGATGCGAGA GAAACAGTTT TCCCAGGCAC GACCCCTGAC CCGCTACCTG 
CCAATCCGGA AGGAGGACTT TGACCTGAAG ACCCACATCG AGTCGTCGGG CCACGGTGTC 
GATACCTGCC TGCACGTGGT GCTTAGCAGC AAGGTCTGCC GCGGCTACTT GGTCAAGATG 
GGCGGCAAGA TTAAATCATG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC 
CTTTCCTATT ATGTGGACAA GCACGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC 
ATCGAGGAAG TGTACTACGA TCACCTGCGC AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT 
TCTGTGGTGA CTGAGAGCCC GAACCCAGCC CTCACCTTCT GTGTGAAGAC CCACGACCGG 
CTGTATTACA TGGTGGCCCC GTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATCGTC 
ACGGGAGCGG AGGGCTACAC TCAGTTCATG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273946.1
CDS	86..4201
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418238.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418238.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC 
CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG 
AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC 
CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC 
GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA GACGACCCAG CAGGACAGCA GGTGATCGAG 
GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG 
TGGGACGCCC TGCACGGGGC GGCCCCCTTC CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC 
ATGCACCACT CCATCCTGCA CCACCTGCCG GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG 
CACGCCTACG ACACCCTGAG CCTGGAGAGC TCCGACAGCA TGGAGACCAG CATCTCCACA 
GGCGGCAACT CAGCCTGCTC GCCCGACAAC ATGTCCAGTG CGAGCGGCCT GGATGTGGGC 
AAAATCGAGG AGATGGAGAA GATGCTGAAA GAGGCACACG CCGAGAAGAG CCGGCTCATG 
GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG 
CGAGAACAGG TGGAACGGAG GCTACAGAGC GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG 
AAGGAGGTCA AGATGCGAGA GAAACAGTTT TCCCAGGCAC GACCCCTGAC CCGCTACCTG 
CCAATCCGGA AGGAGGACTT TGACCTGAAG ACCCACATCG AGTCGTCGGG CCACGGTGTC 
GATACCTGCC TGCACGTGGT GCTTAGCAGC AAGGTCTGCC GCGGCTACTT GGTCAAGATG 
GGCGGCAAGA TTAAATCATG GAAGAAGCGC TGGTTTGTCT TCGACCGGCT CAAGCGCACC 
CTTTCCTATT ATGTGGACAA GCACGAGACG AAGCTGAAGG GGGTCATCTA CTTCCAGGCC 
ATCGAGGAAG TGTACTACGA TCACCTGCGC AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT 
TCTGTGGTGA CTGAGAGCCC GAACCCAGCC CTCACCTTCT GTGTGAAGAC CCACGACCGG 
CTGTATTACA TGGTGGCCCC GTCTGCAGAG GCCATGCGCA TCTGGATGGA CGTCATCGTC 
ACGGGAGCGG AGGGCTACAC TCAGTTCATG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619707.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619764.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe05896
Clone ID Related Accession (Same CDS sequence)	XM_005619707.2
Accession Version	XM_005619707.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619707.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGTTTACC GCTCCAAGAT GGATGGTGAG GCCACCAGCC CCCTGCCCCG GACCCGCAGT 
GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT TCCTCTTCTT CGCAGCTCAG CGTGGCTACT 
CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC CTACTTGCCC AGAATGGCAC AGGCAGCCTT 
CCTCGCAACC TGGCGGCCAC GCTGCAGGAC ATTGAGACCA AGCGCCAGCT GGCCCTGCAG 
CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC CCCCCAGCAG ACGACCCAGC AGGACAGCAG 
GTGATCGAGG AGCAGCGGCG GCGCCTGGCC GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG 
CAGTGCCAGT GGGACGCCCT GCACGGGGCG GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC 
CCCCCGCTCA TGCACCACTC CATCCTGCAC CACCTGCCGG CTGGCCGGGA GCGCGGGGAG 
GAGGGCGAGC ACGCCTACGA CACCCTGAGC CTGGAGAGCT CCGACAGCAT GGAGACCAGC 
ATCTCCACAG GCGGCAACTC AGCCTGCTCG CCCGACAACA TGTCCAGTGC GAGCGGCCTG 
GATGTGGGCA AAATCGAGGA GATGGAGAAG ATGCTGAAAG AGGCACACGC CGAGAAGAGC 
CGGCTCATGG AGTCCAGGGA GCGGGAGATG GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA 
CGGAGGAGGC GAGAACAGGT GGAACGGAGG CTACAGAGCG AGAGCGCCAG GAGGCAGCAG 
CTGGTGGAGA AGGAGGTCAA GATGCGAGAG AAACAGTTTT CCCAGGCACG ACCCCTGACC 
CGCTACCTGC CAATCCGGAA GGAGGACTTT GACCTGAAGA CCCACATCGA GTCGTCGGGC 
CACGGTGTCG ATACCTGCCT GCACGTGGTG CTTAGCAGCA AGGTCTGCCG CGGCTACTTG 
GTCAAGATGG GCGGCAAGAT TAAATCATGG AAGAAGCGCT GGTTTGTCTT CGACCGGCTC 
AAGCGCACCC TTTCCTATTA TGTGGACAAG CACGAGACGA AGCTGAAGGG GGTCATCTAC 
TTCCAGGCCA TCGAGGAAGT GTACTACGAT CACCTGCGCA GTGCAGCCAA GAGGAGGTTT 
TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG AACCCAGCCC TCACCTTCTG TGTGAAGACC 
CACGACCGGC TGTATTACAT GGTGGCCCCG TCTGCAGAGG CCATGCGCAT CTGGATGGAC 
GTCATCGTCA CGGGAGCGGA GGGCTACACT CAGTTCATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619764.1
CDS	412..4656
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619707.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619707.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGTTTACC GCTCCAAGAT GGATGGTGAG GCCACCAGCC CCCTGCCCCG GACCCGCAGT 
GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT TCCTCTTCTT CGCAGCTCAG CGTGGCTACT 
CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC CTACTTGCCC AGAATGGCAC AGGCAGCCTT 
CCTCGCAACC TGGCGGCCAC GCTGCAGGAC ATTGAGACCA AGCGCCAGCT GGCCCTGCAG 
CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC CCCCCAGCAG ACGACCCAGC AGGACAGCAG 
GTGATCGAGG AGCAGCGGCG GCGCCTGGCC GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG 
CAGTGCCAGT GGGACGCCCT GCACGGGGCG GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC 
CCCCCGCTCA TGCACCACTC CATCCTGCAC CACCTGCCGG CTGGCCGGGA GCGCGGGGAG 
GAGGGCGAGC ACGCCTACGA CACCCTGAGC CTGGAGAGCT CCGACAGCAT GGAGACCAGC 
ATCTCCACAG GCGGCAACTC AGCCTGCTCG CCCGACAACA TGTCCAGTGC GAGCGGCCTG 
GATGTGGGCA AAATCGAGGA GATGGAGAAG ATGCTGAAAG AGGCACACGC CGAGAAGAGC 
CGGCTCATGG AGTCCAGGGA GCGGGAGATG GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA 
CGGAGGAGGC GAGAACAGGT GGAACGGAGG CTACAGAGCG AGAGCGCCAG GAGGCAGCAG 
CTGGTGGAGA AGGAGGTCAA GATGCGAGAG AAACAGTTTT CCCAGGCACG ACCCCTGACC 
CGCTACCTGC CAATCCGGAA GGAGGACTTT GACCTGAAGA CCCACATCGA GTCGTCGGGC 
CACGGTGTCG ATACCTGCCT GCACGTGGTG CTTAGCAGCA AGGTCTGCCG CGGCTACTTG 
GTCAAGATGG GCGGCAAGAT TAAATCATGG AAGAAGCGCT GGTTTGTCTT CGACCGGCTC 
AAGCGCACCC TTTCCTATTA TGTGGACAAG CACGAGACGA AGCTGAAGGG GGTCATCTAC 
TTCCAGGCCA TCGAGGAAGT GTACTACGAT CACCTGCGCA GTGCAGCCAA GAGGAGGTTT 
TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG AACCCAGCCC TCACCTTCTG TGTGAAGACC 
CACGACCGGC TGTATTACAT GGTGGCCCCG TCTGCAGAGG CCATGCGCAT CTGGATGGAC 
GTCATCGTCA CGGGAGCGGA GGGCTACACT CAGTTCATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_855372.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860465.3 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.

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CloneID	OCe05899
Clone ID Related Accession (Same CDS sequence)	XM_855372.4 , XM_855372.5
Accession Version	XM_855372.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_855372.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC 
CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG 
AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC 
CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC 
GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG 
GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC 
GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC 
GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG 
AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG 
CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC 
CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC 
TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC 
CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC 
CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC 
ACTCAGTTCA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860465.3
CDS	406..4443
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_855372.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855372.4

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC 
CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG 
AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC 
CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC 
GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG 
GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC 
GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC 
GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG 
AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG 
CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC 
CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC 
TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC 
CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC 
CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC 
ACTCAGTTCA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_855372.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_860465.3 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.

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CloneID	OCe05899
Clone ID Related Accession (Same CDS sequence)	XM_855372.4 , XM_855372.5
Accession Version	XM_855372.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_855372.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC 
CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG 
AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC 
CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC 
GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG 
GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC 
GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC 
GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG 
AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG 
CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC 
CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC 
TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC 
CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC 
CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC 
ACTCAGTTCA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_860465.3
CDS	85..4122
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_855372.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_855372.5

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGTTTAC 
CGCTCCAAGA TGGATGGTGA GGCCACCAGC CCCCTGCCCC GGACCCGCAG TGGCCCCCTC 
CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG 
AGCCCCTCCC CAAAGAGTGC CCTACTTGCC CAGAATGGCA CAGGCAGCCT TCCTCGCAAC 
CTGGCGGCCA CGCTGCAGGA CATTGAGACC AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA 
CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC 
GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG 
GGCTTCCCCC CGCTCATGCA CCACTCCATC CTGCACCACC TGCCGGCTGG CCGGGAGCGC 
GGGGAGGAGG GCGAGCACGC CTACGACACC CTGAGCCTGG AGAGCTCCGA CAGCATGGAG 
ACCAGCATCT CCACAGGCGG CAACTCAGCC TGCTCGCCCG ACAACATGTC CAGTGCGAGC 
GGCCTGGATG TGGGCAAAAT CGAGGAGATG GAGAAGATGC TGAAAGAGGC ACACGCCGAG 
AAGAGCCGGC TCATGGAGTC CAGGGAGCGG GAGATGGAGC TCCGGCGGCA GGCCCTGGAG 
GAGGAACGGA GGAGGCGAGA ACAGGTGGAA CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG 
CAGCAGCTGG TGGAGAAGGA GGTCAAGATG CGAGAGAAAC AGTTTTCCCA GGCACGACCC 
CTGACCCGCT ACCTGCCAAT CCGGAAGGAG GACTTTGACC TGAAGACCCA CATCGAGTCG 
TCGGGCCACG GTGTCGATAC CTGCCTGCAC GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC 
TACTTGGTCA AGATGGGCGG CAAGATTAAA TCATGGAAGA AGCGCTGGTT TGTCTTCGAC 
CGGCTCAAGC GCACCCTTTC CTATTATGTG GACAAGCACG AGACGAAGCT GAAGGGGGTC 
ATCTACTTCC AGGCCATCGA GGAAGTGTAC TACGATCACC TGCGCAGTGC AGCCAAGAGC 
CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC 
CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC 
ACTCAGTTCA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619699.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619756.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe05888
Clone ID Related Accession (Same CDS sequence)	XM_005619699.2
Accession Version	XM_005619699.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT 
GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG 
CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG 
CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC 
ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC 
ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA 
GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG 
ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG 
CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG 
GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG 
ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG 
GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG 
CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT 
AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT 
GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG 
TACTACGATC ACCTGCGCAG TGCAGCCAAG AGGAGGTTTT TCAGCTTTTC TGTGGTGACT 
GAGAGCCCGA ACCCAGCCCT CACCTTCTGT GTGAAGACCC ACGACCGGCT GTATTACATG 
GTGGCCCCGT CTGCAGAGGC CATGCGCATC TGGATGGACG TCATCGTCAC GGGAGCGGAG 
GGCTACACTC AGTTCATGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619756.1
CDS	419..5122
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619699.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619699.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT 
GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG 
CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG 
CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC 
ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC 
ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA 
GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG 
ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG 
CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG 
GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG 
ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG 
GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG 
CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT 
AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT 
GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG 
TACTACGATC ACCTGCGCAG TGCAGCCAAG AGGAGGTTTT TCAGCTTTTC TGTGGTGACT 
GAGAGCCCGA ACCCAGCCCT CACCTTCTGT GTGAAGACCC ACGACCGGCT GTATTACATG 
GTGGCCCCGT CTGCAGAGGC CATGCGCATC TGGATGGACG TCATCGTCAC GGGAGCGGAG 
GGCTACACTC AGTTCATGAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619700.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619757.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe05889
Clone ID Related Accession (Same CDS sequence)	XM_005619700.2
Accession Version	XM_005619700.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA 
CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA 
ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT 
TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT 
GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC 
TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT 
TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA 
CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT 
CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG 
GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG 
GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT 
CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC 
CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC 
AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG 
CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC 
CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA 
GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC 
GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT 
CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG 
AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC 
CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC 
ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA 
GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG 
AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC 
ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT 
GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG 
TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG 
AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT 
CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG 
GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG 
CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG 
CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG 
GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC 
CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC 
ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG 
GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT 
CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT 
GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT 
TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG 
AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG 
CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA 
GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC 
CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC 
GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC 
TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG 
GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG 
GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA 
CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG 
CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC 
GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA 
TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG 
GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC 
TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG 
AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG 
GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC 
TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619757.1
CDS	419..5119
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619700.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619700.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA 
CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA 
ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT 
TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT 
GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC 
TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT 
TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA 
CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT 
CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG 
GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG 
GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT 
CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC 
CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC 
AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG 
CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC 
CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA 
GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC 
GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT 
CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG 
AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC 
CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC 
ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA 
GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG 
AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC 
ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT 
GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG 
TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG 
AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT 
CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG 
GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG 
CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG 
CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG 
GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC 
CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC 
ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG 
GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT 
CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT 
GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT 
TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG 
AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG 
CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA 
GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC 
CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC 
GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC 
TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG 
GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG 
GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA 
CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG 
CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC 
GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA 
TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG 
GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC 
TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG 
AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG 
GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC 
TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619701.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619758.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.

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CloneID	OCe05890
Clone ID Related Accession (Same CDS sequence)	XM_005619701.2
Accession Version	XM_005619701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT 
GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG 
CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG 
CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC 
ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC 
ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA 
GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG 
ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG 
CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG 
GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG 
ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG 
GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG 
CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT 
AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT 
GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG 
TACTACGATC ACCTGCGCAG TGCAGCCAAG AGCCCGAACC CAGCCCTCAC CTTCTGTGTG 
AAGACCCACG ACCGGCTGTA TTACATGGTG GCCCCGTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TCGTCACGGG AGCGGAGGGC TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619758.1
CDS	417..5087
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619701.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619701.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT 
GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG 
CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG 
CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC 
ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC 
ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA 
GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG 
ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG 
CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG 
GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG 
ATGCGAGAGA AACAGTTTTC CCAGGCACGA CCCCTGACCC GCTACCTGCC AATCCGGAAG 
GAGGACTTTG ACCTGAAGAC CCACATCGAG TCGTCGGGCC ACGGTGTCGA TACCTGCCTG 
CACGTGGTGC TTAGCAGCAA GGTCTGCCGC GGCTACTTGG TCAAGATGGG CGGCAAGATT 
AAATCATGGA AGAAGCGCTG GTTTGTCTTC GACCGGCTCA AGCGCACCCT TTCCTATTAT 
GTGGACAAGC ACGAGACGAA GCTGAAGGGG GTCATCTACT TCCAGGCCAT CGAGGAAGTG 
TACTACGATC ACCTGCGCAG TGCAGCCAAG AGCCCGAACC CAGCCCTCAC CTTCTGTGTG 
AAGACCCACG ACCGGCTGTA TTACATGGTG GCCCCGTCTG CAGAGGCCAT GCGCATCTGG 
ATGGACGTCA TCGTCACGGG AGCGGAGGGC TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619702.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619759.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.

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CloneID	OCe05891
Clone ID Related Accession (Same CDS sequence)	XM_005619702.2
Accession Version	XM_005619702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGACA GCAGGTGATC 
GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC 
CAGTGGGACG CCCTGCACGG GGCGGCCCCC TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG 
CTCATGCACC ACTCCATCCT GCACCACCTG CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC 
GAGCACGCCT ACGACACCCT GAGCCTGGAG AGCTCCGACA GCATGGAGAC CAGCATCTCC 
ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC AACATGTCCA GTGCGAGCGG CCTGGATGTG 
GGCAAAATCG AGGAGATGGA GAAGATGCTG AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC 
ATGGAGTCCA GGGAGCGGGA GATGGAGCTC CGGCGGCAGG CCCTGGAGGA GGAACGGAGG 
AGGCGAGAAC AGGTGGAACG GAGGCTACAG AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG 
GAGAAGGAGG TCAAGATGCG AGAGAAACAG TTTTCCCAGG CACGACCCCT GACCCGCTAC 
CTGCCAATCC GGAAGGAGGA CTTTGACCTG AAGACCCACA TCGAGTCGTC GGGCCACGGT 
GTCGATACCT GCCTGCACGT GGTGCTTAGC AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG 
ATGGGCGGCA AGATTAAATC ATGGAAGAAG CGCTGGTTTG TCTTCGACCG GCTCAAGCGC 
ACCCTTTCCT ATTATGTGGA CAAGCACGAG ACGAAGCTGA AGGGGGTCAT CTACTTCCAG 
GCCATCGAGG AAGTGTACTA CGATCACCTG CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC 
TTTTCTGTGG TGACTGAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC 
CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC 
GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619759.1
CDS	418..5076
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619702.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619702.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGACA GCAGGTGATC 
GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC 
CAGTGGGACG CCCTGCACGG GGCGGCCCCC TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG 
CTCATGCACC ACTCCATCCT GCACCACCTG CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC 
GAGCACGCCT ACGACACCCT GAGCCTGGAG AGCTCCGACA GCATGGAGAC CAGCATCTCC 
ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC AACATGTCCA GTGCGAGCGG CCTGGATGTG 
GGCAAAATCG AGGAGATGGA GAAGATGCTG AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC 
ATGGAGTCCA GGGAGCGGGA GATGGAGCTC CGGCGGCAGG CCCTGGAGGA GGAACGGAGG 
AGGCGAGAAC AGGTGGAACG GAGGCTACAG AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG 
GAGAAGGAGG TCAAGATGCG AGAGAAACAG TTTTCCCAGG CACGACCCCT GACCCGCTAC 
CTGCCAATCC GGAAGGAGGA CTTTGACCTG AAGACCCACA TCGAGTCGTC GGGCCACGGT 
GTCGATACCT GCCTGCACGT GGTGCTTAGC AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG 
ATGGGCGGCA AGATTAAATC ATGGAAGAAG CGCTGGTTTG TCTTCGACCG GCTCAAGCGC 
ACCCTTTCCT ATTATGTGGA CAAGCACGAG ACGAAGCTGA AGGGGGTCAT CTACTTCCAG 
GCCATCGAGG AAGTGTACTA CGATCACCTG CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC 
TTTTCTGTGG TGACTGAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC 
CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC 
GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619703.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619760.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.

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CloneID	OCe05892
Clone ID Related Accession (Same CDS sequence)	XM_005619703.2
Accession Version	XM_005619703.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619703.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG 
GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG 
GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC 
AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT 
ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA 
GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC 
TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC 
ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC 
CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT 
GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA 
CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC 
ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG 
CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC 
GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC 
TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG 
GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT 
GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG 
GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC 
CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG 
CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA 
TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT 
CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG 
GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC 
TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC 
CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG 
AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT 
ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA 
CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC 
CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC 
GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG 
GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG 
GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC 
ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG 
GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG 
CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG 
GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG 
CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG 
GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG 
ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG 
GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG 
GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC 
CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT 
CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG 
AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA 
TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT 
GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC 
CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG 
CTCTGCCCCA AAGCACAAGA GTACGTGACG CTTGAGCAGC TAAAGGTGAT GTGGGGCACC 
TCACCCGTGC CCACAGCCCC CGCGCCGGGC CTGCCTTTCT GGGCCTCTGC CTCCCGGGAC 
CTGGTTCCCA CCACCTGCCT TCCTCCCGTG CTGCCCTCTT CCTCCTCCTC CGCTTCTCTC 
ACGCCTTCAC CCAAGATGGA GGAGCTGCTA CTCCCTGCTG TAGACTTAGA GCAGTGGTAC 
CAGGAGCTGA TGGCCGGGCT GGGGACCGGC CCCGCTGCAG CCTCCCCGCG CTCCTCCCCC 
CCGCCTCTGC CCGCCAAAGC TTCCCGGCAG CTGCAGGTTT ACCGCTCCAA GATGGATGGT 
GAGGCCACCA GCCCCCTGCC CCGGACCCGC AGTGGCCCCC TCCCCTCCTC CTCTGGTTCT 
TCTTCCTCTT CTTCGCAGCT CAGCGTGGCT ACTCTGGGCC GGAGCCCCTC CCCAAAGAGT 
GCCCTACTTG CCCAGAATGG CACAGGCAGC CTTCCTCGCA ACCTGGCGGC CACGCTGCAG 
GACATTGAGA CCAAGCGCCA GCTGGCCCTG CAGCAGAAGG GCGAGTCGCT TCCTGCAGAG 
CCCCCCCCAG CAGACGACCC AGCAGGACAG CAGGTGATCG AGGAGCAGCG GCGGCGCCTG 
GCCGAGCTGA AGCAGAAGGC TGCGGCCGAG GCGCAGTGCC AGTGGGACGC CCTGCACGGG 
GCGGCCCCCT TCCCCCCGGG CCCCTCGGGC TTCCCCCCGC TCATGCACCA CTCCATCCTG 
CACCACCTGC CGGCTGGCCG GGAGCGCGGG GAGGAGGGCG AGCACGCCTA CGACACCCTG 
AGCCTGGAGA GCTCCGACAG CATGGAGACC AGCATCTCCA CAGGCGGCAA CTCAGCCTGC 
TCGCCCGACA ACATGTCCAG TGCGAGCGGC CTGGATGTGG GCAAAATCGA GGAGATGGAG 
AAGATGCTGA AAGAGGCACA CGCCGAGAAG AGCCGGCTCA TGGAGTCCAG GGAGCGGGAG 
ATGGAGCTCC GGCGGCAGGC CCTGGAGGAG GAACGGAGGA GGCGAGAACA GGTGGAACGG 
AGGCTACAGA GCGAGAGCGC CAGGAGGCAG CAGCTGGTGG AGAAGGAGGT CAAGATGCGA 
GAGAAACAGT TTTCCCAGGC ACGACCCCTG ACCCGCTACC TGCCAATCCG GAAGGAGGAC 
TTTGACCTGA AGACCCACAT CGAGTCGTCG GGCCACGGTG TCGATACCTG CCTGCACGTG 
GTGCTTAGCA GCAAGGTCTG CCGCGGCTAC TTGGTCAAGA TGGGCGGCAA GATTAAATCA 
TGGAAGAAGC GCTGGTTTGT CTTCGACCGG CTCAAGCGCA CCCTTTCCTA TTATGTGGAC 
AAGCACGAGA CGAAGCTGAA GGGGGTCATC TACTTCCAGG CCATCGAGGA AGTGTACTAC 
GATCACCTGC GCAGTGCAGC CAAGAGGAGG TTTTTCAGCT TTTCTGTGGT GACTGAGAGC 
CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC 
CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC 
ACTCAGTTCA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619760.1
CDS	324..4961
Translation	MDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619703.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619703.2

ORF Insert Sequence:

ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG 
GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG 
GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC 
AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT 
ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA 
GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC 
TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC 
ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC 
CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT 
GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA 
CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC 
ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG 
CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC 
GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC 
TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG 
GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT 
GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG 
GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC 
CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG 
CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA 
TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT 
CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG 
GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC 
TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC 
CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG 
AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT 
ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA 
CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC 
CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC 
GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG 
GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG 
GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC 
ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG 
GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG 
CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG 
GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG 
CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG 
GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG 
ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG 
GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG 
GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC 
CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT 
CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG 
AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA 
TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT 
GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC 
CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG 
CTCTGCCCCA AAGCACAAGA GTACGTGACG CTTGAGCAGC TAAAGGTGAT GTGGGGCACC 
TCACCCGTGC CCACAGCCCC CGCGCCGGGC CTGCCTTTCT GGGCCTCTGC CTCCCGGGAC 
CTGGTTCCCA CCACCTGCCT TCCTCCCGTG CTGCCCTCTT CCTCCTCCTC CGCTTCTCTC 
ACGCCTTCAC CCAAGATGGA GGAGCTGCTA CTCCCTGCTG TAGACTTAGA GCAGTGGTAC 
CAGGAGCTGA TGGCCGGGCT GGGGACCGGC CCCGCTGCAG CCTCCCCGCG CTCCTCCCCC 
CCGCCTCTGC CCGCCAAAGC TTCCCGGCAG CTGCAGGTTT ACCGCTCCAA GATGGATGGT 
GAGGCCACCA GCCCCCTGCC CCGGACCCGC AGTGGCCCCC TCCCCTCCTC CTCTGGTTCT 
TCTTCCTCTT CTTCGCAGCT CAGCGTGGCT ACTCTGGGCC GGAGCCCCTC CCCAAAGAGT 
GCCCTACTTG CCCAGAATGG CACAGGCAGC CTTCCTCGCA ACCTGGCGGC CACGCTGCAG 
GACATTGAGA CCAAGCGCCA GCTGGCCCTG CAGCAGAAGG GCGAGTCGCT TCCTGCAGAG 
CCCCCCCCAG CAGACGACCC AGCAGGACAG CAGGTGATCG AGGAGCAGCG GCGGCGCCTG 
GCCGAGCTGA AGCAGAAGGC TGCGGCCGAG GCGCAGTGCC AGTGGGACGC CCTGCACGGG 
GCGGCCCCCT TCCCCCCGGG CCCCTCGGGC TTCCCCCCGC TCATGCACCA CTCCATCCTG 
CACCACCTGC CGGCTGGCCG GGAGCGCGGG GAGGAGGGCG AGCACGCCTA CGACACCCTG 
AGCCTGGAGA GCTCCGACAG CATGGAGACC AGCATCTCCA CAGGCGGCAA CTCAGCCTGC 
TCGCCCGACA ACATGTCCAG TGCGAGCGGC CTGGATGTGG GCAAAATCGA GGAGATGGAG 
AAGATGCTGA AAGAGGCACA CGCCGAGAAG AGCCGGCTCA TGGAGTCCAG GGAGCGGGAG 
ATGGAGCTCC GGCGGCAGGC CCTGGAGGAG GAACGGAGGA GGCGAGAACA GGTGGAACGG 
AGGCTACAGA GCGAGAGCGC CAGGAGGCAG CAGCTGGTGG AGAAGGAGGT CAAGATGCGA 
GAGAAACAGT TTTCCCAGGC ACGACCCCTG ACCCGCTACC TGCCAATCCG GAAGGAGGAC 
TTTGACCTGA AGACCCACAT CGAGTCGTCG GGCCACGGTG TCGATACCTG CCTGCACGTG 
GTGCTTAGCA GCAAGGTCTG CCGCGGCTAC TTGGTCAAGA TGGGCGGCAA GATTAAATCA 
TGGAAGAAGC GCTGGTTTGT CTTCGACCGG CTCAAGCGCA CCCTTTCCTA TTATGTGGAC 
AAGCACGAGA CGAAGCTGAA GGGGGTCATC TACTTCCAGG CCATCGAGGA AGTGTACTAC 
GATCACCTGC GCAGTGCAGC CAAGAGGAGG TTTTTCAGCT TTTCTGTGGT GACTGAGAGC 
CCGAACCCAG CCCTCACCTT CTGTGTGAAG ACCCACGACC GGCTGTATTA CATGGTGGCC 
CCGTCTGCAG AGGCCATGCG CATCTGGATG GACGTCATCG TCACGGGAGC GGAGGGCTAC 
ACTCAGTTCA TGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619704.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619761.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.

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CloneID	OCe05893
Clone ID Related Accession (Same CDS sequence)	XM_005619704.2
Accession Version	XM_005619704.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4575bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619704.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCA 
CCCGTGCCCA CAGCCCCCGC GCCGGGCCTG CCTTTCTGGG CCTCTGCCTC CCGGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGTGCTG CCCTCTTCCT CCTCCTCCGC TTCTCTCACG 
CCTTCACCCA AGATGGAGGA GCTGCTACTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACCGGCCCC GCTGCAGCCT CCCCGCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGGCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGTGAG 
GCCACCAGCC CCCTGCCCCG GACCCGCAGT GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT 
TCCTCTTCTT CGCAGCTCAG CGTGGCTACT CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC 
CTACTTGCCC AGAATGGCAC AGGCAGCCTT CCTCGCAACC TGGCGGCCAC GCTGCAGGAC 
ATTGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC 
CCCCCAGCAG ACGACCCAGC AGGACAGCAG GTGATCGAGG AGCAGCGGCG GCGCCTGGCC 
GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG CAGTGCCAGT GGGACGCCCT GCACGGGGCG 
GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC CCCCCGCTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CTGGCCGGGA GCGCGGGGAG GAGGGCGAGC ACGCCTACGA CACCCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACAG GCGGCAACTC AGCCTGCTCG 
CCCGACAACA TGTCCAGTGC GAGCGGCCTG GATGTGGGCA AAATCGAGGA GATGGAGAAG 
ATGCTGAAAG AGGCACACGC CGAGAAGAGC CGGCTCATGG AGTCCAGGGA GCGGGAGATG 
GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA CGGAGGAGGC GAGAACAGGT GGAACGGAGG 
CTACAGAGCG AGAGCGCCAG GAGGCAGCAG CTGGTGGAGA AGGAGGTCAA GATGCGAGAG 
AAACAGTTTT CCCAGGCACG ACCCCTGACC CGCTACCTGC CAATCCGGAA GGAGGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCGGGC CACGGTGTCG ATACCTGCCT GCACGTGGTG 
CTTAGCAGCA AGGTCTGCCG CGGCTACTTG GTCAAGATGG GCGGCAAGAT TAAATCATGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA TGTGGACAAG 
CACGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAAGT GTACTACGAT 
CACCTGCGCA GTGCAGCCAA GAGGAGGTTT TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG 
AACCCAGCCC TCACCTTCTG TGTGAAGACC CACGACCGGC TGTATTACAT GGTGGCCCCG 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATCGTCA CGGGAGCGGA GGGCTACACT 
CAGTTCATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619761.1
CDS	417..4991
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619704.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619704.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGTA CGTGACGCTT GAGCAGCTAA AGGTGATGTG GGGCACCTCA 
CCCGTGCCCA CAGCCCCCGC GCCGGGCCTG CCTTTCTGGG CCTCTGCCTC CCGGGACCTG 
GTTCCCACCA CCTGCCTTCC TCCCGTGCTG CCCTCTTCCT CCTCCTCCGC TTCTCTCACG 
CCTTCACCCA AGATGGAGGA GCTGCTACTC CCTGCTGTAG ACTTAGAGCA GTGGTACCAG 
GAGCTGATGG CCGGGCTGGG GACCGGCCCC GCTGCAGCCT CCCCGCGCTC CTCCCCCCCG 
CCTCTGCCCG CCAAAGCTTC CCGGCAGCTG CAGGTTTACC GCTCCAAGAT GGATGGTGAG 
GCCACCAGCC CCCTGCCCCG GACCCGCAGT GGCCCCCTCC CCTCCTCCTC TGGTTCTTCT 
TCCTCTTCTT CGCAGCTCAG CGTGGCTACT CTGGGCCGGA GCCCCTCCCC AAAGAGTGCC 
CTACTTGCCC AGAATGGCAC AGGCAGCCTT CCTCGCAACC TGGCGGCCAC GCTGCAGGAC 
ATTGAGACCA AGCGCCAGCT GGCCCTGCAG CAGAAGGGCG AGTCGCTTCC TGCAGAGCCC 
CCCCCAGCAG ACGACCCAGC AGGACAGCAG GTGATCGAGG AGCAGCGGCG GCGCCTGGCC 
GAGCTGAAGC AGAAGGCTGC GGCCGAGGCG CAGTGCCAGT GGGACGCCCT GCACGGGGCG 
GCCCCCTTCC CCCCGGGCCC CTCGGGCTTC CCCCCGCTCA TGCACCACTC CATCCTGCAC 
CACCTGCCGG CTGGCCGGGA GCGCGGGGAG GAGGGCGAGC ACGCCTACGA CACCCTGAGC 
CTGGAGAGCT CCGACAGCAT GGAGACCAGC ATCTCCACAG GCGGCAACTC AGCCTGCTCG 
CCCGACAACA TGTCCAGTGC GAGCGGCCTG GATGTGGGCA AAATCGAGGA GATGGAGAAG 
ATGCTGAAAG AGGCACACGC CGAGAAGAGC CGGCTCATGG AGTCCAGGGA GCGGGAGATG 
GAGCTCCGGC GGCAGGCCCT GGAGGAGGAA CGGAGGAGGC GAGAACAGGT GGAACGGAGG 
CTACAGAGCG AGAGCGCCAG GAGGCAGCAG CTGGTGGAGA AGGAGGTCAA GATGCGAGAG 
AAACAGTTTT CCCAGGCACG ACCCCTGACC CGCTACCTGC CAATCCGGAA GGAGGACTTT 
GACCTGAAGA CCCACATCGA GTCGTCGGGC CACGGTGTCG ATACCTGCCT GCACGTGGTG 
CTTAGCAGCA AGGTCTGCCG CGGCTACTTG GTCAAGATGG GCGGCAAGAT TAAATCATGG 
AAGAAGCGCT GGTTTGTCTT CGACCGGCTC AAGCGCACCC TTTCCTATTA TGTGGACAAG 
CACGAGACGA AGCTGAAGGG GGTCATCTAC TTCCAGGCCA TCGAGGAAGT GTACTACGAT 
CACCTGCGCA GTGCAGCCAA GAGGAGGTTT TTCAGCTTTT CTGTGGTGAC TGAGAGCCCG 
AACCCAGCCC TCACCTTCTG TGTGAAGACC CACGACCGGC TGTATTACAT GGTGGCCCCG 
TCTGCAGAGG CCATGCGCAT CTGGATGGAC GTCATCGTCA CGGGAGCGGA GGGCTACACT 
CAGTTCATGA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619705.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619762.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe05894
Clone ID Related Accession (Same CDS sequence)	XM_005619705.2
Accession Version	XM_005619705.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619705.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGATG GAGGAGCTGC TACTCCCTGC TGTAGACTTA 
GAGCAGTGGT ACCAGGAGCT GATGGCCGGG CTGGGGACCG GCCCCGCTGC AGCCTCCCCG 
CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA GCTTCCCGGC AGCTGCAGGT TTACCGCTCC 
AAGATGGATG GTGAGGCCAC CAGCCCCCTG CCCCGGACCC GCAGTGGCCC CCTCCCCTCC 
TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG CTCAGCGTGG CTACTCTGGG CCGGAGCCCC 
TCCCCAAAGA GTGCCCTACT TGCCCAGAAT GGCACAGGCA GCCTTCCTCG CAACCTGGCG 
GCCACGCTGC AGGACATTGA GACCAAGCGC CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG 
CTTCCTGCAG AGCCCCCCCC AGCAGACGAC CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC 
GCCCTGCACG GGGCGGCCCC CTTCCCCCCG GGCCCCTCGG GCTTCCCCCC GCTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCTGGC CGGGAGCGCG GGGAGGAGGG CGAGCACGCC 
TACGACACCC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACAGGCGGC 
AACTCAGCCT GCTCGCCCGA CAACATGTCC AGTGCGAGCG GCCTGGATGT GGGCAAAATC 
GAGGAGATGG AGAAGATGCT GAAAGAGGCA CACGCCGAGA AGAGCCGGCT CATGGAGTCC 
AGGGAGCGGG AGATGGAGCT CCGGCGGCAG GCCCTGGAGG AGGAACGGAG GAGGCGAGAA 
CAGGTGGAAC GGAGGCTACA GAGCGAGAGC GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG 
GTCAAGATGC GAGAGAAACA GTTTTCCCAG GCACGACCCC TGACCCGCTA CCTGCCAATC 
CGGAAGGAGG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CGGGCCACGG TGTCGATACC 
TGCCTGCACG TGGTGCTTAG CAGCAAGGTC TGCCGCGGCT ACTTGGTCAA GATGGGCGGC 
AAGATTAAAT CATGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTATGTGG ACAAGCACGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAAGTGTACT ACGATCACCT GCGCAGTGCA GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG 
GTGACTGAGA GCCCGAACCC AGCCCTCACC TTCTGTGTGA AGACCCACGA CCGGCTGTAT 
TACATGGTGG CCCCGTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT CGTCACGGGA 
GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619762.1
CDS	417..4946
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619705.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619705.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGATG GAGGAGCTGC TACTCCCTGC TGTAGACTTA 
GAGCAGTGGT ACCAGGAGCT GATGGCCGGG CTGGGGACCG GCCCCGCTGC AGCCTCCCCG 
CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA GCTTCCCGGC AGCTGCAGGT TTACCGCTCC 
AAGATGGATG GTGAGGCCAC CAGCCCCCTG CCCCGGACCC GCAGTGGCCC CCTCCCCTCC 
TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG CTCAGCGTGG CTACTCTGGG CCGGAGCCCC 
TCCCCAAAGA GTGCCCTACT TGCCCAGAAT GGCACAGGCA GCCTTCCTCG CAACCTGGCG 
GCCACGCTGC AGGACATTGA GACCAAGCGC CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG 
CTTCCTGCAG AGCCCCCCCC AGCAGACGAC CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG 
CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC 
GCCCTGCACG GGGCGGCCCC CTTCCCCCCG GGCCCCTCGG GCTTCCCCCC GCTCATGCAC 
CACTCCATCC TGCACCACCT GCCGGCTGGC CGGGAGCGCG GGGAGGAGGG CGAGCACGCC 
TACGACACCC TGAGCCTGGA GAGCTCCGAC AGCATGGAGA CCAGCATCTC CACAGGCGGC 
AACTCAGCCT GCTCGCCCGA CAACATGTCC AGTGCGAGCG GCCTGGATGT GGGCAAAATC 
GAGGAGATGG AGAAGATGCT GAAAGAGGCA CACGCCGAGA AGAGCCGGCT CATGGAGTCC 
AGGGAGCGGG AGATGGAGCT CCGGCGGCAG GCCCTGGAGG AGGAACGGAG GAGGCGAGAA 
CAGGTGGAAC GGAGGCTACA GAGCGAGAGC GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG 
GTCAAGATGC GAGAGAAACA GTTTTCCCAG GCACGACCCC TGACCCGCTA CCTGCCAATC 
CGGAAGGAGG ACTTTGACCT GAAGACCCAC ATCGAGTCGT CGGGCCACGG TGTCGATACC 
TGCCTGCACG TGGTGCTTAG CAGCAAGGTC TGCCGCGGCT ACTTGGTCAA GATGGGCGGC 
AAGATTAAAT CATGGAAGAA GCGCTGGTTT GTCTTCGACC GGCTCAAGCG CACCCTTTCC 
TATTATGTGG ACAAGCACGA GACGAAGCTG AAGGGGGTCA TCTACTTCCA GGCCATCGAG 
GAAGTGTACT ACGATCACCT GCGCAGTGCA GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG 
GTGACTGAGA GCCCGAACCC AGCCCTCACC TTCTGTGTGA AGACCCACGA CCGGCTGTAT 
TACATGGTGG CCCCGTCTGC AGAGGCCATG CGCATCTGGA TGGACGTCAT CGTCACGGGA 
GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619706.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619763.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.

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CloneID	OCe05895
Clone ID Related Accession (Same CDS sequence)	XM_005619706.2
Accession Version	XM_005619706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC 
AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC 
AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC 
CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619763.1
CDS	414..4799
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619706.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619706.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC 
AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC 
AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC 
CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_546499.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_546499.3 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.

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CloneID	OCe05897
Clone ID Related Accession (Same CDS sequence)	XM_546499.4 , XM_546499.5
Accession Version	XM_546499.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_546499.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC 
CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG 
TTCATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_546499.3
CDS	410..4621
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_546499.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_546499.4

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC 
CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG 
TTCATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_546499.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_546499.3 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.

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CloneID	OCe05897
Clone ID Related Accession (Same CDS sequence)	XM_546499.4 , XM_546499.5
Accession Version	XM_546499.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_546499.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC 
CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG 
TTCATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_546499.3
CDS	87..4298
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_546499.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_546499.5

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG GAGGTTTTTC AGCTTTTCTG TGGTGACTGA GAGCCCGAAC 
CCAGCCCTCA CCTTCTGTGT GAAGACCCAC GACCGGCTGT ATTACATGGT GGCCCCGTCT 
GCAGAGGCCA TGCGCATCTG GATGGACGTC ATCGTCACGG GAGCGGAGGG CTACACTCAG 
TTCATGAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619708.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619765.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.

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CloneID	OCe05898
Clone ID Related Accession (Same CDS sequence)	XM_005619708.3 , XM_005619708.2
Accession Version	XM_005619708.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619708.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC 
CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC 
GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619765.1
CDS	86..4264
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619708.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619708.3

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC 
CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC 
GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_005619708.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005619765.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.

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CloneID	OCe05898
Clone ID Related Accession (Same CDS sequence)	XM_005619708.3 , XM_005619708.2
Accession Version	XM_005619708.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005619708.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC 
CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC 
GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005619765.1
CDS	409..4587
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_005619708.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005619708.2

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG ACAGCAGGTG ATCGAGGAGC AGCGGCGGCG CCTGGCCGAG 
CTGAAGCAGA AGGCTGCGGC CGAGGCGCAG TGCCAGTGGG ACGCCCTGCA CGGGGCGGCC 
CCCTTCCCCC CGGGCCCCTC GGGCTTCCCC CCGCTCATGC ACCACTCCAT CCTGCACCAC 
CTGCCGGCTG GCCGGGAGCG CGGGGAGGAG GGCGAGCACG CCTACGACAC CCTGAGCCTG 
GAGAGCTCCG ACAGCATGGA GACCAGCATC TCCACAGGCG GCAACTCAGC CTGCTCGCCC 
GACAACATGT CCAGTGCGAG CGGCCTGGAT GTGGGCAAAA TCGAGGAGAT GGAGAAGATG 
CTGAAAGAGG CACACGCCGA GAAGAGCCGG CTCATGGAGT CCAGGGAGCG GGAGATGGAG 
CTCCGGCGGC AGGCCCTGGA GGAGGAACGG AGGAGGCGAG AACAGGTGGA ACGGAGGCTA 
CAGAGCGAGA GCGCCAGGAG GCAGCAGCTG GTGGAGAAGG AGGTCAAGAT GCGAGAGAAA 
CAGTTTTCCC AGGCACGACC CCTGACCCGC TACCTGCCAA TCCGGAAGGA GGACTTTGAC 
CTGAAGACCC ACATCGAGTC GTCGGGCCAC GGTGTCGATA CCTGCCTGCA CGTGGTGCTT 
AGCAGCAAGG TCTGCCGCGG CTACTTGGTC AAGATGGGCG GCAAGATTAA ATCATGGAAG 
AAGCGCTGGT TTGTCTTCGA CCGGCTCAAG CGCACCCTTT CCTATTATGT GGACAAGCAC 
GAGACGAAGC TGAAGGGGGT CATCTACTTC CAGGCCATCG AGGAAGTGTA CTACGATCAC 
CTGCGCAGTG CAGCCAAGAG CCCGAACCCA GCCCTCACCT TCTGTGTGAA GACCCACGAC 
CGGCTGTATT ACATGGTGGC CCCGTCTGCA GAGGCCATGC GCATCTGGAT GGACGTCATC 
GTCACGGGAG CGGAGGGCTA CACTCAGTTC ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418225.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273933.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.

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CloneID	OCe287933
Clone ID Related Accession (Same CDS sequence)	XM_022418225.1
Accession Version	XM_022418225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC 
AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC 
AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC 
CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273933.1
CDS	364..5289
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418225.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418225.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC 
AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC 
AGTGCAGCCA AGAGGAGGTT TTTCAGCTTT TCTGTGGTGA CTGAGAGCCC GAACCCAGCC 
CTCACCTTCT GTGTGAAGAC CCACGACCGG CTGTATTACA TGGTGGCCCC GTCTGCAGAG 
GCCATGCGCA TCTGGATGGA CGTCATCGTC ACGGGAGCGG AGGGCTACAC TCAGTTCATG 
AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418226.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273934.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe287934
Clone ID Related Accession (Same CDS sequence)	XM_022418226.1
Accession Version	XM_022418226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA 
CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA 
ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT 
TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT 
GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC 
TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT 
TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA 
CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT 
CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG 
GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG 
GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT 
CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC 
CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC 
AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG 
CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC 
CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA 
GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC 
GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT 
CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG 
AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC 
CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC 
ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA 
GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG 
AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC 
ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT 
GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG 
TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG 
AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT 
CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG 
GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG 
CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG 
CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG 
GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC 
CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA 
AGGGTCAGTT TCACCAGCCC CCCTTCCCTG GCCCCCGAGG GCCACCCACC CAGAAGAGAG 
GAGAGCAGTG GGACGGGACC ACCCCGGGCC CTGCAGTACC TGCCGGACCC GGACACCAGG 
GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG 
GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC 
CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT 
CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG 
AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA 
TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT 
GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC 
CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG 
CTCTGCCCCA AAGCACAAGA GGAGTATGTG AGCCTGGCAG AGGTTCTCCA GCTGTGCTTC 
CGCTTGGACC CCCATGCTTC CGCCACCTCC CCCAGCGTGT TCGCCCAGCC CCTGCCCGAC 
AGTGAGTACG TGACGCTTGA GCAGCTAAAG GTGATGTGGG GCACCTCACC CGTGCCCACA 
GCCCCCGCGC CGGGCCTGCC TTTCTGGGCC TCTGCCTCCC GGGACCTGGT TCCCACCACC 
TGCCTTCCTC CCGTGCTGCC CTCTTCCTCC TCCTCCGCTT CTCTCACGCC TTCACCCAAG 
ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC 
GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC 
AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC 
CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG 
CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG 
AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG 
CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC 
GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG 
AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC 
CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT 
GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG 
TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG 
GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG 
AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC 
CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG 
GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT 
GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC 
ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273934.1
CDS	362..5284
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418226.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418226.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGAAT CAGAAAGCCT GGTGAACGGG AACCACACCC CACAGCCTGC CACCCGGGGA 
CCCTCCGCCT GTGCCAGCCA CAGCTCCCTG GTGAGCTCTA TTGAGAAGGA CCTGCAGGAA 
ATCATGGACT CACTGGTGCT AGAGGAGCCT GGGGCGGCAG GCAAGAAGCC TGCTGCGACT 
TCCCCACTGT CGCCGATGGC TAACGGTGGG CGCTACTTGC TGTCCCCGCC GACCAGCCCT 
GGTGCCATGT CCGTGGGCTC CAGCTATGAG AACACCTCTC CAGCCTTCTC TCCACTCTCC 
TCACCAGCCA GCAGTGGAAG CTGTGCCAGC CACTCACCCA GCGGGCAGGA GCCAGCACCT 
TCCATGCCCC CGCTGGTCCC TGCCCGTTCC TCCAGCTACC ATCTGGCTCT GCAGCCCCCA 
CAGCCCCGAC CGAGTGGTGC CCGCCCCTCT GAGAGCCCCC GGTTGGGCAG GAAAGGGGGT 
CACGAGAGGC CTCCCAGTCC TGGCCTCCGA GGTCTGCTGA CAGACAGCCC TGCAGCCACG 
GTCTTGGCTG AGGCCCGCAG AGCCACTGAG AGCCCCCGGC TGGGTGGACA GCTGCCCGTG 
GTGGCCATCA GCCTGAGTGA GTACCCGGCT TCCGGTGCCC GAAGCCAACC CACCAGCATT 
CCTGGCAGCC CCAAGTTCCA GCCTCCAGTC CCCGCTCCCC GAAACAAGAT CGGCACCCTC 
CAGGACCGTC CTCCCAGCCC TTTCCGTGAG CTGCCCAGCA CCGAGCGGGT GCTGACAACC 
AGCCCCTCAC GCCAGCTGGT GGGCCGGACA TTTTCAGATG GGTCAGTCGC CCGCACTCTG 
CAGCCCCCCG AGAGCCCCCG CCTGGGCCGG CGGGGCCTGG ACAGTATGCG GGAATTGCCC 
CCATTGAGCC CGTCACTGTC CCGACGGGCG CTGTCCCCGA TGCCCACCAG GACTACTCCA 
GATCCCAAGC TAACCAGAGA AGTGGCTGAG AGTCCCCGGC CCCGGCGCTG GGCAGCCCAC 
GGGGCATCAC CAGAGGACTT CTCACTGACC CTGGGGGCAC GGGGCCGTAG GACTCGGAGT 
CCCTCACCCA CACTTGGGGA GTCCCTGGCG CCCCGCAAGG GCAGCTTCAG TGGCAGGCTG 
AGCCCGGCCT ACAGTCTGGG CTCTCTGACA GGGGCTTCGC CCCGTCAGAG CCCCCGCGCC 
CAGAGGAAGC TGTCCAGTGG GGACCTGCGG GTACCTGTCA CTCGGGAGCG GAAAAATAGC 
ATTACGGAGA TCAGCGACAA TGAGGACGAC CTCCTGGAGT ACCACCGGCG GCAGCGCCAA 
GAACGGCTCT GGGAGCAGGA GATGGAGAGG CTGGAGCGCC AGCGCCTGGA GACCATACTG 
AACCTGTGTG CCGAGTACAG CCGTGCTGAT GGGGGCCTCG AGGCGGGGGA GCTGCCTAGC 
ATCGGGGAGG CCGCCGCGGC ACTGGCGCTG GCGGGCCGCA GGCCCTCACG AGGCCTTTCT 
GGGGCCCCCG GAGCCTCGGG GCGGAGCACT GAAGAGCCTG GGGGTGCCAC CCCGCGCCTG 
TGGGAGTGTG TGGAGCGCTC AGATGAGGAG AACCTGAAGG AGGAGTGCAG CAGCACGGAG 
AGCACCCAGC AGGAGCACGA AGATGCCCCT AGCACCAAGC TTCAGGGAGA GGTGCTGGCT 
CTGGAAGAGG AGCGGGCTCA GGTGCTGGGG CGTGTGGAGC AGCTCAAAGT CCGCGTGAAG 
GAGCTGGAGC AGCAGCTGCA GGAGTCAGCC CGAGAGGCTG AGATGGAACG GGCGTTGCTG 
CAGGGGGAGA GGGAGGCAGA GCGGGCACTG CTGCAGAAGG AGCAGAAGGC GGTGGACCAG 
CTGCAGGAGA AGTTAGTGAC CTTGGAGACT GGCATCCAGA AGGAGAGGGA CAAGGAGAGG 
GCGGAGCTGG CCGCGGGACG GAGGCACCTG GAGGCCCGCC AGGCGCTCTA CGCCGAGCTC 
CAGACGCAGC TCGATAACTG CCCCGAGTCA GTGCGGGAAC AGTTACAGGA GCAGCTGAGA 
AGGGTCAGTT TCACCAGCCC CCCTTCCCTG GCCCCCGAGG GCCACCCACC CAGAAGAGAG 
GAGAGCAGTG GGACGGGACC ACCCCGGGCC CTGCAGTACC TGCCGGACCC GGACACCAGG 
GAGGCCGAGG CCCTGGAGAC GGAGACAAAG CTGTTTGAGG ACTTGGAGTT CCAGCAGCTG 
GAGCGGGAGA GCCGCGTGGA GGAGGAGCGG GAGCTGGCAG GCCAGGGGCT GCTCCGGAGC 
CAGGCCGAGC TGCTCCGCAG CATCACCAAG AGGAAGGAGC GCCTGGCAGT CCTGGACAGT 
CAGGCGGGGC AGATCCGGGC CCAGGCCGTG CAGGAGTCCG AGCGCCTGGC GAGGGACAAG 
AACGCTTCCC TGCAGCTGCT GCAGAAGGAG AAGGAGAGAC TGACTATGTT GGAGGGAAGA 
TACCACTCAC TCACAGGAGG CAGGCCCTTC CCGAAGACCA CGTCCACCCT CAAAGAGGCT 
GAACTGCTCA TCTCCGAGTC CTCAGAGATG GGGCTGGGGT CGGCGACTCT GGTCCCTTTC 
CCAGGGTCTT CTCAGCCCGG GGCCTTCTCT GTCCCCCTAA CCCAACCTGC TTCCACTCAG 
CTCTGCCCCA AAGCACAAGA GGAGTATGTG AGCCTGGCAG AGGTTCTCCA GCTGTGCTTC 
CGCTTGGACC CCCATGCTTC CGCCACCTCC CCCAGCGTGT TCGCCCAGCC CCTGCCCGAC 
AGTGAGTACG TGACGCTTGA GCAGCTAAAG GTGATGTGGG GCACCTCACC CGTGCCCACA 
GCCCCCGCGC CGGGCCTGCC TTTCTGGGCC TCTGCCTCCC GGGACCTGGT TCCCACCACC 
TGCCTTCCTC CCGTGCTGCC CTCTTCCTCC TCCTCCGCTT CTCTCACGCC TTCACCCAAG 
ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC 
GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC 
AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC 
CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG 
CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG 
AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG 
CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC 
GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG 
AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC 
CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT 
GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG 
TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG 
GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG 
AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC 
CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG 
GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT 
GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC 
ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418227.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273935.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.

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CloneID	OCe287935
Clone ID Related Accession (Same CDS sequence)	XM_022418227.1
Accession Version	XM_022418227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC 
AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC 
AGTGCAGCCA AGAGCCCGAA CCCAGCCCTC ACCTTCTGTG TGAAGACCCA CGACCGGCTG 
TATTACATGG TGGCCCCGTC TGCAGAGGCC ATGCGCATCT GGATGGACGT CATCGTCACG 
GGAGCGGAGG GCTACACTCA GTTCATGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273935.1
CDS	91..4983
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKSPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418227.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418227.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGGCAC GACCCCTGAC CCGCTACCTG CCAATCCGGA AGGAGGACTT TGACCTGAAG 
ACCCACATCG AGTCGTCGGG CCACGGTGTC GATACCTGCC TGCACGTGGT GCTTAGCAGC 
AAGGTCTGCC GCGGCTACTT GGTCAAGATG GGCGGCAAGA TTAAATCATG GAAGAAGCGC 
TGGTTTGTCT TCGACCGGCT CAAGCGCACC CTTTCCTATT ATGTGGACAA GCACGAGACG 
AAGCTGAAGG GGGTCATCTA CTTCCAGGCC ATCGAGGAAG TGTACTACGA TCACCTGCGC 
AGTGCAGCCA AGAGCCCGAA CCCAGCCCTC ACCTTCTGTG TGAAGACCCA CGACCGGCTG 
TATTACATGG TGGCCCCGTC TGCAGAGGCC ATGCGCATCT GGATGGACGT CATCGTCACG 
GGAGCGGAGG GCTACACTCA GTTCATGAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418228.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273936.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.

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CloneID	OCe287936
Clone ID Related Accession (Same CDS sequence)	XM_022418228.1
Accession Version	XM_022418228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC 
CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC 
GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC 
TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG 
GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG 
GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA 
CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG 
CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC 
GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA 
TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG 
GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC 
TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG 
AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG 
GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC 
TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273936.1
CDS	360..5240
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418228.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418228.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC 
CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC 
GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC 
TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG 
GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG 
GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA 
CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG 
CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC 
GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA 
TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG 
GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC 
TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG 
AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG 
GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC 
TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418229.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273937.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.

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CloneID	OCe287937
Clone ID Related Accession (Same CDS sequence)	XM_022418229.1
Accession Version	XM_022418229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG 
GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG 
GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC 
AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT 
ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA 
GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC 
TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC 
ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC 
CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT 
GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA 
CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC 
ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG 
CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC 
GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC 
TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG 
GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT 
GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG 
GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC 
CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG 
CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA 
TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT 
CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG 
GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC 
TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC 
CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG 
AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT 
ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA 
CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC 
CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC 
GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG 
GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG 
GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC 
ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG 
GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG 
CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG 
GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG 
CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG 
GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG 
ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG 
GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG 
AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC 
TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273937.1
CDS	322..5181
Translation	MDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418229.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418229.1

ORF Insert Sequence:

ATGGATGCTC TTAATAGGAA CCAGGTAGGC CCTGGATGCA AGACCCAAGC AATGGTAAAG 
AAAGGGCCCT TGGACCTGAT CGAGACAGGC AAAGGGCTGA AGGTGCAAAC GGACAAACCC 
CATCTGGTGA GCCTGGGCAG TGGGCGGCTG AGCACGGCTA TCACCCTTCT GCCTCTGGAG 
GAAGGGAAGA CGGTGATTGG CTCCGCAGCC AGGGACATCT CCCTGCAGGG CCCAGGCTTG 
GCTCCGGAGC ACTGCTACAT CGAGAACGTG CGGGGCACCC TCACCCTCTA CCCTTGTGGC 
AATGCCTGCA GTATTGACGG GCTCCCCATC CGGCAGCCCA CCAGGCTCAC TCAGGGCTGT 
ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA 
GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC 
TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC 
ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC 
CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT 
GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA 
CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC 
ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG 
CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC 
GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC 
TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG 
GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT 
GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG 
GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC 
CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG 
CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA 
TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT 
CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG 
GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC 
TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC 
CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG 
AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT 
ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA 
CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC 
CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC 
GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG 
GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG 
GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC 
ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG 
GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG 
CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG 
GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG 
CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG 
GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG 
ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG 
GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG 
AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC 
TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418230.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273938.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.

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CloneID	OCe287938
Clone ID Related Accession (Same CDS sequence)	XM_022418230.1
Accession Version	XM_022418230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC 
ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG 
GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT 
CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT 
GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT 
TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG 
AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG 
CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA 
GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC 
CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC 
GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC 
TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG 
GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG 
GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA 
CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG 
CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC 
GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA 
TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG 
GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC 
TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG 
AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG 
GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC 
TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273938.1
CDS	359..5179
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418230.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418230.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGTACGTG ACGCTTGAGC AGCTAAAGGT GATGTGGGGC 
ACCTCACCCG TGCCCACAGC CCCCGCGCCG GGCCTGCCTT TCTGGGCCTC TGCCTCCCGG 
GACCTGGTTC CCACCACCTG CCTTCCTCCC GTGCTGCCCT CTTCCTCCTC CTCCGCTTCT 
CTCACGCCTT CACCCAAGAT GGAGGAGCTG CTACTCCCTG CTGTAGACTT AGAGCAGTGG 
TACCAGGAGC TGATGGCCGG GCTGGGGACC GGCCCCGCTG CAGCCTCCCC GCGCTCCTCC 
CCCCCGCCTC TGCCCGCCAA AGCTTCCCGG CAGCTGCAGG TTTACCGCTC CAAGATGGAT 
GGTGAGGCCA CCAGCCCCCT GCCCCGGACC CGCAGTGGCC CCCTCCCCTC CTCCTCTGGT 
TCTTCTTCCT CTTCTTCGCA GCTCAGCGTG GCTACTCTGG GCCGGAGCCC CTCCCCAAAG 
AGTGCCCTAC TTGCCCAGAA TGGCACAGGC AGCCTTCCTC GCAACCTGGC GGCCACGCTG 
CAGGACATTG AGACCAAGCG CCAGCTGGCC CTGCAGCAGA AGGGCGAGTC GCTTCCTGCA 
GAGCCCCCCC CAGCAGACGA CCCAGCAGGA CAGCAGGTGA TCGAGGAGCA GCGGCGGCGC 
CTGGCCGAGC TGAAGCAGAA GGCTGCGGCC GAGGCGCAGT GCCAGTGGGA CGCCCTGCAC 
GGGGCGGCCC CCTTCCCCCC GGGCCCCTCG GGCTTCCCCC CGCTCATGCA CCACTCCATC 
CTGCACCACC TGCCGGCTGG CCGGGAGCGC GGGGAGGAGG GCGAGCACGC CTACGACACC 
CTGAGCCTGG AGAGCTCCGA CAGCATGGAG ACCAGCATCT CCACAGGCGG CAACTCAGCC 
TGCTCGCCCG ACAACATGTC CAGTGCGAGC GGCCTGGATG TGGGCAAAAT CGAGGAGATG 
GAGAAGATGC TGAAAGAGGC ACACGCCGAG AAGAGCCGGC TCATGGAGTC CAGGGAGCGG 
GAGATGGAGC TCCGGCGGCA GGCCCTGGAG GAGGAACGGA GGAGGCGAGA ACAGGTGGAA 
CGGAGGCTAC AGAGCGAGAG CGCCAGGAGG CAGCAGCTGG TGGAGAAGGA GGTCAAGATG 
CGAGAGAAAC AGTTTTCCCA GGCACGACCC CTGACCCGCT ACCTGCCAAT CCGGAAGGAG 
GACTTTGACC TGAAGACCCA CATCGAGTCG TCGGGCCACG GTGTCGATAC CTGCCTGCAC 
GTGGTGCTTA GCAGCAAGGT CTGCCGCGGC TACTTGGTCA AGATGGGCGG CAAGATTAAA 
TCATGGAAGA AGCGCTGGTT TGTCTTCGAC CGGCTCAAGC GCACCCTTTC CTATTATGTG 
GACAAGCACG AGACGAAGCT GAAGGGGGTC ATCTACTTCC AGGCCATCGA GGAAGTGTAC 
TACGATCACC TGCGCAGTGC AGCCAAGAGG AGGTTTTTCA GCTTTTCTGT GGTGACTGAG 
AGCCCGAACC CAGCCCTCAC CTTCTGTGTG AAGACCCACG ACCGGCTGTA TTACATGGTG 
GCCCCGTCTG CAGAGGCCAT GCGCATCTGG ATGGACGTCA TCGTCACGGG AGCGGAGGGC 
TACACTCAGT TCATGAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418231.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273939.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe287939
Clone ID Related Accession (Same CDS sequence)	XM_022418231.1
Accession Version	XM_022418231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGGAG TATGTGAGCC TGGCAGAGGT TCTCCAGCTG 
TGCTTCCGCT TGGACCCCCA TGCTTCCGCC ACCTCCCCCA GCGTGTTCGC CCAGCCCCTG 
CCCGACAGTG AGTACGTGAC GCTTGAGCAG CTAAAGGTGA TGTGGGGCAC CTCACCCGTG 
CCCACAGCCC CCGCGCCGGG CCTGCCTTTC TGGGCCTCTG CCTCCCGGGA CCTGGTTCCC 
ACCACCTGCC TTCCTCCCGT GCTGCCCTCT TCCTCCTCCT CCGCTTCTCT CACGCCTTCA 
CCCAAGATGG AGGAGCTGCT ACTCCCTGCT GTAGACTTAG AGCAGTGGTA CCAGGAGCTG 
ATGGCCGGGC TGGGGACCGG CCCCGCTGCA GCCTCCCCGC GCTCCTCCCC CCCGCCTCTG 
CCCGCCAAAG CTTCCCGGCA GCTGCAGGTT TACCGCTCCA AGATGGATGG TGAGGCCACC 
AGCCCCCTGC CCCGGACCCG CAGTGGCCCC CTCCCCTCCT CCTCTGGTTC TTCTTCCTCT 
TCTTCGCAGC TCAGCGTGGC TACTCTGGGC CGGAGCCCCT CCCCAAAGAG TGCCCTACTT 
GCCCAGAATG GCACAGGCAG CCTTCCTCGC AACCTGGCGG CCACGCTGCA GGACATTGAG 
ACCAAGCGCC AGCTGGCCCT GCAGCAGAAG GGCGAGTCGC TTCCTGCAGA GCCCCCCCCA 
GCAGACGACC CAGCAGGACA GCAGGTGATC GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG 
AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC CAGTGGGACG CCCTGCACGG GGCGGCCCCC 
TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG CTCATGCACC ACTCCATCCT GCACCACCTG 
CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC GAGCACGCCT ACGACACCCT GAGCCTGGAG 
AGCTCCGACA GCATGGAGAC CAGCATCTCC ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC 
AACATGTCCA GTGCGAGCGG CCTGGATGTG GGCAAAATCG AGGAGATGGA GAAGATGCTG 
AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC ATGGAGTCCA GGGAGCGGGA GATGGAGCTC 
CGGCGGCAGG CCCTGGAGGA GGAACGGAGG AGGCGAGAAC AGGTGGAACG GAGGCTACAG 
AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG GAGAAGGAGG TCAAGATGCG AGAGAAACAG 
TTTTCCCAGG CACGACCCCT GACCCGCTAC CTGCCAATCC GGAAGGAGGA CTTTGACCTG 
AAGACCCACA TCGAGTCGTC GGGCCACGGT GTCGATACCT GCCTGCACGT GGTGCTTAGC 
AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG ATGGGCGGCA AGATTAAATC ATGGAAGAAG 
CGCTGGTTTG TCTTCGACCG GCTCAAGCGC ACCCTTTCCT ATTATGTGGA CAAGCACGAG 
ACGAAGCTGA AGGGGGTCAT CTACTTCCAG GCCATCGAGG AAGTGTACTA CGATCACCTG 
CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC TTTTCTGTGG TGACTGAGAG CCCGAACCCA 
GCCCTCACCT TCTGTGTGAA GACCCACGAC CGGCTGTATT ACATGGTGGC CCCGTCTGCA 
GAGGCCATGC GCATCTGGAT GGACGTCATC GTCACGGGAG CGGAGGGCTA CACTCAGTTC 
ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273939.1
CDS	91..4899
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418231.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418231.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGGAG TATGTGAGCC TGGCAGAGGT TCTCCAGCTG 
TGCTTCCGCT TGGACCCCCA TGCTTCCGCC ACCTCCCCCA GCGTGTTCGC CCAGCCCCTG 
CCCGACAGTG AGTACGTGAC GCTTGAGCAG CTAAAGGTGA TGTGGGGCAC CTCACCCGTG 
CCCACAGCCC CCGCGCCGGG CCTGCCTTTC TGGGCCTCTG CCTCCCGGGA CCTGGTTCCC 
ACCACCTGCC TTCCTCCCGT GCTGCCCTCT TCCTCCTCCT CCGCTTCTCT CACGCCTTCA 
CCCAAGATGG AGGAGCTGCT ACTCCCTGCT GTAGACTTAG AGCAGTGGTA CCAGGAGCTG 
ATGGCCGGGC TGGGGACCGG CCCCGCTGCA GCCTCCCCGC GCTCCTCCCC CCCGCCTCTG 
CCCGCCAAAG CTTCCCGGCA GCTGCAGGTT TACCGCTCCA AGATGGATGG TGAGGCCACC 
AGCCCCCTGC CCCGGACCCG CAGTGGCCCC CTCCCCTCCT CCTCTGGTTC TTCTTCCTCT 
TCTTCGCAGC TCAGCGTGGC TACTCTGGGC CGGAGCCCCT CCCCAAAGAG TGCCCTACTT 
GCCCAGAATG GCACAGGCAG CCTTCCTCGC AACCTGGCGG CCACGCTGCA GGACATTGAG 
ACCAAGCGCC AGCTGGCCCT GCAGCAGAAG GGCGAGTCGC TTCCTGCAGA GCCCCCCCCA 
GCAGACGACC CAGCAGGACA GCAGGTGATC GAGGAGCAGC GGCGGCGCCT GGCCGAGCTG 
AAGCAGAAGG CTGCGGCCGA GGCGCAGTGC CAGTGGGACG CCCTGCACGG GGCGGCCCCC 
TTCCCCCCGG GCCCCTCGGG CTTCCCCCCG CTCATGCACC ACTCCATCCT GCACCACCTG 
CCGGCTGGCC GGGAGCGCGG GGAGGAGGGC GAGCACGCCT ACGACACCCT GAGCCTGGAG 
AGCTCCGACA GCATGGAGAC CAGCATCTCC ACAGGCGGCA ACTCAGCCTG CTCGCCCGAC 
AACATGTCCA GTGCGAGCGG CCTGGATGTG GGCAAAATCG AGGAGATGGA GAAGATGCTG 
AAAGAGGCAC ACGCCGAGAA GAGCCGGCTC ATGGAGTCCA GGGAGCGGGA GATGGAGCTC 
CGGCGGCAGG CCCTGGAGGA GGAACGGAGG AGGCGAGAAC AGGTGGAACG GAGGCTACAG 
AGCGAGAGCG CCAGGAGGCA GCAGCTGGTG GAGAAGGAGG TCAAGATGCG AGAGAAACAG 
TTTTCCCAGG CACGACCCCT GACCCGCTAC CTGCCAATCC GGAAGGAGGA CTTTGACCTG 
AAGACCCACA TCGAGTCGTC GGGCCACGGT GTCGATACCT GCCTGCACGT GGTGCTTAGC 
AGCAAGGTCT GCCGCGGCTA CTTGGTCAAG ATGGGCGGCA AGATTAAATC ATGGAAGAAG 
CGCTGGTTTG TCTTCGACCG GCTCAAGCGC ACCCTTTCCT ATTATGTGGA CAAGCACGAG 
ACGAAGCTGA AGGGGGTCAT CTACTTCCAG GCCATCGAGG AAGTGTACTA CGATCACCTG 
CGCAGTGCAG CCAAGAGGAG GTTTTTCAGC TTTTCTGTGG TGACTGAGAG CCCGAACCCA 
GCCCTCACCT TCTGTGTGAA GACCCACGAC CGGCTGTATT ACATGGTGGC CCCGTCTGCA 
GAGGCCATGC GCATCTGGAT GGACGTCATC GTCACGGGAG CGGAGGGCTA CACTCAGTTC 
ATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418232.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273940.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe287940
Clone ID Related Accession (Same CDS sequence)	XM_022418232.1
Accession Version	XM_022418232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC 
TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273940.1
CDS	90..4769
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418232.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418232.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC 
TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418233.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273941.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.

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CloneID	OCe287941
Clone ID Related Accession (Same CDS sequence)	XM_022418233.1
Accession Version	XM_022418233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
TACGTGACGC TTGAGCAGCT AAAGGTGATG TGGGGCACCT CACCCGTGCC CACAGCCCCC 
GCGCCGGGCC TGCCTTTCTG GGCCTCTGCC TCCCGGGACC TGGTTCCCAC CACCTGCCTT 
CCTCCCGTGC TGCCCTCTTC CTCCTCCTCC GCTTCTCTCA CGCCTTCACC CAAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG CGAGTCGCTT CCTGCAGAGC CCCCCCCAGC AGACGACCCA 
GCAGGACAGC AGGTGATCGA GGAGCAGCGG CGGCGCCTGG CCGAGCTGAA GCAGAAGGCT 
GCGGCCGAGG CGCAGTGCCA GTGGGACGCC CTGCACGGGG CGGCCCCCTT CCCCCCGGGC 
CCCTCGGGCT TCCCCCCGCT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCTGGCCGG 
GAGCGCGGGG AGGAGGGCGA GCACGCCTAC GACACCCTGA GCCTGGAGAG CTCCGACAGC 
ATGGAGACCA GCATCTCCAC AGGCGGCAAC TCAGCCTGCT CGCCCGACAA CATGTCCAGT 
GCGAGCGGCC TGGATGTGGG CAAAATCGAG GAGATGGAGA AGATGCTGAA AGAGGCACAC 
GCCGAGAAGA GCCGGCTCAT GGAGTCCAGG GAGCGGGAGA TGGAGCTCCG GCGGCAGGCC 
CTGGAGGAGG AACGGAGGAG GCGAGAACAG GTGGAACGGA GGCTACAGAG CGAGAGCGCC 
AGGAGGCAGC AGCTGGTGGA GAAGGAGGTC AAGATGCGAG AGAAACAGTT TTCCCAGGCA 
CGACCCCTGA CCCGCTACCT GCCAATCCGG AAGGAGGACT TTGACCTGAA GACCCACATC 
GAGTCGTCGG GCCACGGTGT CGATACCTGC CTGCACGTGG TGCTTAGCAG CAAGGTCTGC 
CGCGGCTACT TGGTCAAGAT GGGCGGCAAG ATTAAATCAT GGAAGAAGCG CTGGTTTGTC 
TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TATGTGGACA AGCACGAGAC GAAGCTGAAG 
GGGGTCATCT ACTTCCAGGC CATCGAGGAA GTGTACTACG ATCACCTGCG CAGTGCAGCC 
AAGAGGAGGT TTTTCAGCTT TTCTGTGGTG ACTGAGAGCC CGAACCCAGC CCTCACCTTC 
TGTGTGAAGA CCCACGACCG GCTGTATTAC ATGGTGGCCC CGTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATCGT CACGGGAGCG GAGGGCTACA CTCAGTTCAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273941.1
CDS	91..4767
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418233.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418233.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
TACGTGACGC TTGAGCAGCT AAAGGTGATG TGGGGCACCT CACCCGTGCC CACAGCCCCC 
GCGCCGGGCC TGCCTTTCTG GGCCTCTGCC TCCCGGGACC TGGTTCCCAC CACCTGCCTT 
CCTCCCGTGC TGCCCTCTTC CTCCTCCTCC GCTTCTCTCA CGCCTTCACC CAAGATGGAG 
GAGCTGCTAC TCCCTGCTGT AGACTTAGAG CAGTGGTACC AGGAGCTGAT GGCCGGGCTG 
GGGACCGGCC CCGCTGCAGC CTCCCCGCGC TCCTCCCCCC CGCCTCTGCC CGCCAAAGCT 
TCCCGGCAGC TGCAGGTTTA CCGCTCCAAG ATGGATGGTG AGGCCACCAG CCCCCTGCCC 
CGGACCCGCA GTGGCCCCCT CCCCTCCTCC TCTGGTTCTT CTTCCTCTTC TTCGCAGCTC 
AGCGTGGCTA CTCTGGGCCG GAGCCCCTCC CCAAAGAGTG CCCTACTTGC CCAGAATGGC 
ACAGGCAGCC TTCCTCGCAA CCTGGCGGCC ACGCTGCAGG ACATTGAGAC CAAGCGCCAG 
CTGGCCCTGC AGCAGAAGGG CGAGTCGCTT CCTGCAGAGC CCCCCCCAGC AGACGACCCA 
GCAGGACAGC AGGTGATCGA GGAGCAGCGG CGGCGCCTGG CCGAGCTGAA GCAGAAGGCT 
GCGGCCGAGG CGCAGTGCCA GTGGGACGCC CTGCACGGGG CGGCCCCCTT CCCCCCGGGC 
CCCTCGGGCT TCCCCCCGCT CATGCACCAC TCCATCCTGC ACCACCTGCC GGCTGGCCGG 
GAGCGCGGGG AGGAGGGCGA GCACGCCTAC GACACCCTGA GCCTGGAGAG CTCCGACAGC 
ATGGAGACCA GCATCTCCAC AGGCGGCAAC TCAGCCTGCT CGCCCGACAA CATGTCCAGT 
GCGAGCGGCC TGGATGTGGG CAAAATCGAG GAGATGGAGA AGATGCTGAA AGAGGCACAC 
GCCGAGAAGA GCCGGCTCAT GGAGTCCAGG GAGCGGGAGA TGGAGCTCCG GCGGCAGGCC 
CTGGAGGAGG AACGGAGGAG GCGAGAACAG GTGGAACGGA GGCTACAGAG CGAGAGCGCC 
AGGAGGCAGC AGCTGGTGGA GAAGGAGGTC AAGATGCGAG AGAAACAGTT TTCCCAGGCA 
CGACCCCTGA CCCGCTACCT GCCAATCCGG AAGGAGGACT TTGACCTGAA GACCCACATC 
GAGTCGTCGG GCCACGGTGT CGATACCTGC CTGCACGTGG TGCTTAGCAG CAAGGTCTGC 
CGCGGCTACT TGGTCAAGAT GGGCGGCAAG ATTAAATCAT GGAAGAAGCG CTGGTTTGTC 
TTCGACCGGC TCAAGCGCAC CCTTTCCTAT TATGTGGACA AGCACGAGAC GAAGCTGAAG 
GGGGTCATCT ACTTCCAGGC CATCGAGGAA GTGTACTACG ATCACCTGCG CAGTGCAGCC 
AAGAGGAGGT TTTTCAGCTT TTCTGTGGTG ACTGAGAGCC CGAACCCAGC CCTCACCTTC 
TGTGTGAAGA CCCACGACCG GCTGTATTAC ATGGTGGCCC CGTCTGCAGA GGCCATGCGC 
ATCTGGATGG ACGTCATCGT CACGGGAGCG GAGGGCTACA CTCAGTTCAT GAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418234.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273942.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.

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CloneID	OCe287942
Clone ID Related Accession (Same CDS sequence)	XM_022418234.1
Accession Version	XM_022418234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4503bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC 
GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC 
AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC 
CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG 
CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG 
AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG 
CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC 
GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG 
AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC 
CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT 
GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG 
TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG 
GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG 
AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC 
CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG 
GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT 
GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC 
ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273942.1
CDS	90..4592
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418234.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418234.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
ATGGAGGAGC TGCTACTCCC TGCTGTAGAC TTAGAGCAGT GGTACCAGGA GCTGATGGCC 
GGGCTGGGGA CCGGCCCCGC TGCAGCCTCC CCGCGCTCCT CCCCCCCGCC TCTGCCCGCC 
AAAGCTTCCC GGCAGCTGCA GGTTTACCGC TCCAAGATGG ATGGTGAGGC CACCAGCCCC 
CTGCCCCGGA CCCGCAGTGG CCCCCTCCCC TCCTCCTCTG GTTCTTCTTC CTCTTCTTCG 
CAGCTCAGCG TGGCTACTCT GGGCCGGAGC CCCTCCCCAA AGAGTGCCCT ACTTGCCCAG 
AATGGCACAG GCAGCCTTCC TCGCAACCTG GCGGCCACGC TGCAGGACAT TGAGACCAAG 
CGCCAGCTGG CCCTGCAGCA GAAGGGCGAG TCGCTTCCTG CAGAGCCCCC CCCAGCAGAC 
GACCCAGCAG GACAGCAGGT GATCGAGGAG CAGCGGCGGC GCCTGGCCGA GCTGAAGCAG 
AAGGCTGCGG CCGAGGCGCA GTGCCAGTGG GACGCCCTGC ACGGGGCGGC CCCCTTCCCC 
CCGGGCCCCT CGGGCTTCCC CCCGCTCATG CACCACTCCA TCCTGCACCA CCTGCCGGCT 
GGCCGGGAGC GCGGGGAGGA GGGCGAGCAC GCCTACGACA CCCTGAGCCT GGAGAGCTCC 
GACAGCATGG AGACCAGCAT CTCCACAGGC GGCAACTCAG CCTGCTCGCC CGACAACATG 
TCCAGTGCGA GCGGCCTGGA TGTGGGCAAA ATCGAGGAGA TGGAGAAGAT GCTGAAAGAG 
GCACACGCCG AGAAGAGCCG GCTCATGGAG TCCAGGGAGC GGGAGATGGA GCTCCGGCGG 
CAGGCCCTGG AGGAGGAACG GAGGAGGCGA GAACAGGTGG AACGGAGGCT ACAGAGCGAG 
AGCGCCAGGA GGCAGCAGCT GGTGGAGAAG GAGGTCAAGA TGCGAGAGAA ACAGTTTTCC 
CAGGCACGAC CCCTGACCCG CTACCTGCCA ATCCGGAAGG AGGACTTTGA CCTGAAGACC 
CACATCGAGT CGTCGGGCCA CGGTGTCGAT ACCTGCCTGC ACGTGGTGCT TAGCAGCAAG 
GTCTGCCGCG GCTACTTGGT CAAGATGGGC GGCAAGATTA AATCATGGAA GAAGCGCTGG 
TTTGTCTTCG ACCGGCTCAA GCGCACCCTT TCCTATTATG TGGACAAGCA CGAGACGAAG 
CTGAAGGGGG TCATCTACTT CCAGGCCATC GAGGAAGTGT ACTACGATCA CCTGCGCAGT 
GCAGCCAAGA GGAGGTTTTT CAGCTTTTCT GTGGTGACTG AGAGCCCGAA CCCAGCCCTC 
ACCTTCTGTG TGAAGACCCA CGACCGGCTG TATTACATGG TGGCCCCGTC TGCAGAGGCC 
ATGCGCATCT GGATGGACGT CATCGTCACG GGAGCGGAGG GCTACACTCA GTTCATGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418235.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273943.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe287943
Clone ID Related Accession (Same CDS sequence)	XM_022418235.1
Accession Version	XM_022418235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA 
GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC 
TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC 
ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC 
CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT 
GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA 
CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC 
ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG 
CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC 
GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC 
TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG 
GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT 
GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG 
GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC 
CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG 
CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA 
TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT 
CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG 
GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC 
TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC 
CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG 
AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT 
ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA 
CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC 
CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC 
GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG 
GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG 
GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC 
ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG 
GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG 
CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG 
GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG 
CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG 
GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG 
ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG 
GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG 
AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC 
TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273943.1
CDS	140..4639
Translation	MVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_022418235.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418235.1

ORF Insert Sequence:

ATGGTGTGCC TGGGTCAGTC CACCTTCCTC CGTTTTAACC ACCCAGCTGA AGCCAAGTGG 
ATGAAGAGCA TGATTCCAGC AGGGGGCCGG GCCCCAGGGC CCCCCTACAG TCCTGGCTCA 
GCAGAATCAG AAAGCCTGGT GAACGGGAAC CACACCCCAC AGCCTGCCAC CCGGGGACCC 
TCCGCCTGTG CCAGCCACAG CTCCCTGGTG AGCTCTATTG AGAAGGACCT GCAGGAAATC 
ATGGACTCAC TGGTGCTAGA GGAGCCTGGG GCGGCAGGCA AGAAGCCTGC TGCGACTTCC 
CCACTGTCGC CGATGGCTAA CGGTGGGCGC TACTTGCTGT CCCCGCCGAC CAGCCCTGGT 
GCCATGTCCG TGGGCTCCAG CTATGAGAAC ACCTCTCCAG CCTTCTCTCC ACTCTCCTCA 
CCAGCCAGCA GTGGAAGCTG TGCCAGCCAC TCACCCAGCG GGCAGGAGCC AGCACCTTCC 
ATGCCCCCGC TGGTCCCTGC CCGTTCCTCC AGCTACCATC TGGCTCTGCA GCCCCCACAG 
CCCCGACCGA GTGGTGCCCG CCCCTCTGAG AGCCCCCGGT TGGGCAGGAA AGGGGGTCAC 
GAGAGGCCTC CCAGTCCTGG CCTCCGAGGT CTGCTGACAG ACAGCCCTGC AGCCACGGTC 
TTGGCTGAGG CCCGCAGAGC CACTGAGAGC CCCCGGCTGG GTGGACAGCT GCCCGTGGTG 
GCCATCAGCC TGAGTGAGTA CCCGGCTTCC GGTGCCCGAA GCCAACCCAC CAGCATTCCT 
GGCAGCCCCA AGTTCCAGCC TCCAGTCCCC GCTCCCCGAA ACAAGATCGG CACCCTCCAG 
GACCGTCCTC CCAGCCCTTT CCGTGAGCTG CCCAGCACCG AGCGGGTGCT GACAACCAGC 
CCCTCACGCC AGCTGGTGGG CCGGACATTT TCAGATGGGT CAGTCGCCCG CACTCTGCAG 
CCCCCCGAGA GCCCCCGCCT GGGCCGGCGG GGCCTGGACA GTATGCGGGA ATTGCCCCCA 
TTGAGCCCGT CACTGTCCCG ACGGGCGCTG TCCCCGATGC CCACCAGGAC TACTCCAGAT 
CCCAAGCTAA CCAGAGAAGT GGCTGAGAGT CCCCGGCCCC GGCGCTGGGC AGCCCACGGG 
GCATCACCAG AGGACTTCTC ACTGACCCTG GGGGCACGGG GCCGTAGGAC TCGGAGTCCC 
TCACCCACAC TTGGGGAGTC CCTGGCGCCC CGCAAGGGCA GCTTCAGTGG CAGGCTGAGC 
CCGGCCTACA GTCTGGGCTC TCTGACAGGG GCTTCGCCCC GTCAGAGCCC CCGCGCCCAG 
AGGAAGCTGT CCAGTGGGGA CCTGCGGGTA CCTGTCACTC GGGAGCGGAA AAATAGCATT 
ACGGAGATCA GCGACAATGA GGACGACCTC CTGGAGTACC ACCGGCGGCA GCGCCAAGAA 
CGGCTCTGGG AGCAGGAGAT GGAGAGGCTG GAGCGCCAGC GCCTGGAGAC CATACTGAAC 
CTGTGTGCCG AGTACAGCCG TGCTGATGGG GGCCTCGAGG CGGGGGAGCT GCCTAGCATC 
GGGGAGGCCG CCGCGGCACT GGCGCTGGCG GGCCGCAGGC CCTCACGAGG CCTTTCTGGG 
GCCCCCGGAG CCTCGGGGCG GAGCACTGAA GAGCCTGGGG GTGCCACCCC GCGCCTGTGG 
GAGTGTGTGG AGCGCTCAGA TGAGGAGAAC CTGAAGGAGG AGTGCAGCAG CACGGAGAGC 
ACCCAGCAGG AGCACGAAGA TGCCCCTAGC ACCAAGCTTC AGGGAGAGGT GCTGGCTCTG 
GAAGAGGAGC GGGCTCAGGT GCTGGGGCGT GTGGAGCAGC TCAAAGTCCG CGTGAAGGAG 
CTGGAGCAGC AGCTGCAGGA GTCAGCCCGA GAGGCTGAGA TGGAACGGGC GTTGCTGCAG 
GGGGAGAGGG AGGCAGAGCG GGCACTGCTG CAGAAGGAGC AGAAGGCGGT GGACCAGCTG 
CAGGAGAAGT TAGTGACCTT GGAGACTGGC ATCCAGAAGG AGAGGGACAA GGAGAGGGCG 
GAGCTGGCCG CGGGACGGAG GCACCTGGAG GCCCGCCAGG CGCTCTACGC CGAGCTCCAG 
ACGCAGCTCG ATAACTGCCC CGAGTCAGTG CGGGAACAGT TACAGGAGCA GCTGAGAAGG 
GTCAGTTTCA CCAGCCCCCC TTCCCTGGCC CCCGAGGGCC ACCCACCCAG AAGAGAGGAG 
AGCAGTGGGA CGGGACCACC CCGGGCCCTG CAGTACCTGC CGGACCCGGA CACCAGGGAG 
GCCGAGGCCC TGGAGACGGA GACAAAGCTG TTTGAGGACT TGGAGTTCCA GCAGCTGGAG 
CGGGAGAGCC GCGTGGAGGA GGAGCGGGAG CTGGCAGGCC AGGGGCTGCT CCGGAGCCAG 
GCCGAGCTGC TCCGCAGCAT CACCAAGAGG AAGGAGCGCC TGGCAGTCCT GGACAGTCAG 
GCGGGGCAGA TCCGGGCCCA GGCCGTGCAG GAGTCCGAGC GCCTGGCGAG GGACAAGAAC 
GCTTCCCTGC AGCTGCTGCA GAAGGAGAAG GAGAGACTGA CTATGTTGGA GGGAAGATAC 
CACTCACTCA CAGGAGGCAG GCCCTTCCCG AAGACCACGT CCACCCTCAA AGAGGCTGAA 
CTGCTCATCT CCGAGTCCTC AGAGATGGGG CTGGGGTCGG CGACTCTGGT CCCTTTCCCA 
GGGTCTTCTC AGCCCGGGGC CTTCTCTGTC CCCCTAACCC AACCTGCTTC CACTCAGCTC 
TGCCCCAAAG CACAAGAGGA GTATGTGAGC CTGGCAGAGG TTCTCCAGCT GTGCTTCCGC 
TTGGACCCCC ATGCTTCCGC CACCTCCCCC AGCGTGTTCG CCCAGCCCCT GCCCGACAGT 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_022418236.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273944.1 Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe287944
Clone ID Related Accession (Same CDS sequence)	XM_022418236.1
Accession Version	XM_022418236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology-like domain family B member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGCAGG TGGTGTCGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273944.1
CDS	394..4857
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRRVSFTSPPSLAPEGHPPRREESSGTGPPRALQYLPDPDTREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEEYVSLAEVLQLCFRLDPHASATSPSVFAQPLPDSEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQQVVSL

Target ORF information:

RefSeq Version	XM_022418236.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology like domain family B member 1 (PHLDB1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022418236.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGTCA GTTTCACCAG CCCCCCTTCC CTGGCCCCCG AGGGCCACCC ACCCAGAAGA 
GAGGAGAGCA GTGGGACGGG ACCACCCCGG GCCCTGCAGT ACCTGCCGGA CCCGGACACC 
AGGGAGGCCG AGGCCCTGGA GACGGAGACA AAGCTGTTTG AGGACTTGGA GTTCCAGCAG 
CTGGAGCGGG AGAGCCGCGT GGAGGAGGAG CGGGAGCTGG CAGGCCAGGG GCTGCTCCGG 
AGCCAGGCCG AGCTGCTCCG CAGCATCACC AAGAGGAAGG AGCGCCTGGC AGTCCTGGAC 
AGTCAGGCGG GGCAGATCCG GGCCCAGGCC GTGCAGGAGT CCGAGCGCCT GGCGAGGGAC 
AAGAACGCTT CCCTGCAGCT GCTGCAGAAG GAGAAGGAGA GACTGACTAT GTTGGAGGGA 
AGATACCACT CACTCACAGG AGGCAGGCCC TTCCCGAAGA CCACGTCCAC CCTCAAAGAG 
GCTGAACTGC TCATCTCCGA GTCCTCAGAG ATGGGGCTGG GGTCGGCGAC TCTGGTCCCT 
TTCCCAGGGT CTTCTCAGCC CGGGGCCTTC TCTGTCCCCC TAACCCAACC TGCTTCCACT 
CAGCTCTGCC CCAAAGCACA AGAGGAGTAT GTGAGCCTGG CAGAGGTTCT CCAGCTGTGC 
TTCCGCTTGG ACCCCCATGC TTCCGCCACC TCCCCCAGCG TGTTCGCCCA GCCCCTGCCC 
GACAGTGAGT ACGTGACGCT TGAGCAGCTA AAGGTGATGT GGGGCACCTC ACCCGTGCCC 
ACAGCCCCCG CGCCGGGCCT GCCTTTCTGG GCCTCTGCCT CCCGGGACCT GGTTCCCACC 
ACCTGCCTTC CTCCCGTGCT GCCCTCTTCC TCCTCCTCCG CTTCTCTCAC GCCTTCACCC 
AAGATGGAGG AGCTGCTACT CCCTGCTGTA GACTTAGAGC AGTGGTACCA GGAGCTGATG 
GCCGGGCTGG GGACCGGCCC CGCTGCAGCC TCCCCGCGCT CCTCCCCCCC GCCTCTGCCC 
GCCAAAGCTT CCCGGCAGCT GCAGGTTTAC CGCTCCAAGA TGGATGGTGA GGCCACCAGC 
CCCCTGCCCC GGACCCGCAG TGGCCCCCTC CCCTCCTCCT CTGGTTCTTC TTCCTCTTCT 
TCGCAGCTCA GCGTGGCTAC TCTGGGCCGG AGCCCCTCCC CAAAGAGTGC CCTACTTGCC 
CAGAATGGCA CAGGCAGCCT TCCTCGCAAC CTGGCGGCCA CGCTGCAGGA CATTGAGACC 
AAGCGCCAGC TGGCCCTGCA GCAGAAGGGC GAGTCGCTTC CTGCAGAGCC CCCCCCAGCA 
GACGACCCAG CAGGACAGCA GGTGATCGAG GAGCAGCGGC GGCGCCTGGC CGAGCTGAAG 
CAGAAGGCTG CGGCCGAGGC GCAGTGCCAG TGGGACGCCC TGCACGGGGC GGCCCCCTTC 
CCCCCGGGCC CCTCGGGCTT CCCCCCGCTC ATGCACCACT CCATCCTGCA CCACCTGCCG 
GCTGGCCGGG AGCGCGGGGA GGAGGGCGAG CACGCCTACG ACACCCTGAG CCTGGAGAGC 
TCCGACAGCA TGGAGACCAG CATCTCCACA GGCGGCAACT CAGCCTGCTC GCCCGACAAC 
ATGTCCAGTG CGAGCGGCCT GGATGTGGGC AAAATCGAGG AGATGGAGAA GATGCTGAAA 
GAGGCACACG CCGAGAAGAG CCGGCTCATG GAGTCCAGGG AGCGGGAGAT GGAGCTCCGG 
CGGCAGGCCC TGGAGGAGGA ACGGAGGAGG CGAGAACAGG TGGAACGGAG GCTACAGAGC 
GAGAGCGCCA GGAGGCAGCA GCTGGTGGAG AAGGAGGTCA AGATGCGAGA GAAACAGTTT 
TCCCAGCAGG TGGTGTCGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_014113376.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968851.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.

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CloneID	OCe36310
Clone ID Related Accession (Same CDS sequence)	XM_014113376.1
Accession Version	XM_014113376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968851.1
CDS	417..4976
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCRGYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAKRRFFSFSVVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN

Target ORF information:

RefSeq Version	XM_014113376.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113376.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGTACGTGA CGCTTGAGCA GCTAAAGGTG ATGTGGGGCA CCTCACCCGT GCCCACAGCC 
CCCGCGCCGG GCCTGCCTTT CTGGGCCTCT GCCTCCCGGG ACCTGGTTCC CACCACCTGC 
CTTCCTCCCG TGCTGCCCTC TTCCTCCTCC TCCGCTTCTC TCACGCCTTC ACCCAAGATG 
GAGGAGCTGC TACTCCCTGC TGTAGACTTA GAGCAGTGGT ACCAGGAGCT GATGGCCGGG 
CTGGGGACCG GCCCCGCTGC AGCCTCCCCG CGCTCCTCCC CCCCGCCTCT GCCCGCCAAA 
GCTTCCCGGC AGCTGCAGGT TTACCGCTCC AAGATGGATG GTGAGGCCAC CAGCCCCCTG 
CCCCGGACCC GCAGTGGCCC CCTCCCCTCC TCCTCTGGTT CTTCTTCCTC TTCTTCGCAG 
CTCAGCGTGG CTACTCTGGG CCGGAGCCCC TCCCCAAAGA GTGCCCTACT TGCCCAGAAT 
GGCACAGGCA GCCTTCCTCG CAACCTGGCG GCCACGCTGC AGGACATTGA GACCAAGCGC 
CAGCTGGCCC TGCAGCAGAA GGGCGAGTCG CTTCCTGCAG AGCCCCCCCC AGCAGACGAC 
CCAGCAGGAC AGCAGGTGAT CGAGGAGCAG CGGCGGCGCC TGGCCGAGCT GAAGCAGAAG 
GCTGCGGCCG AGGCGCAGTG CCAGTGGGAC GCCCTGCACG GGGCGGCCCC CTTCCCCCCG 
GGCCCCTCGG GCTTCCCCCC GCTCATGCAC CACTCCATCC TGCACCACCT GCCGGCTGGC 
CGGGAGCGCG GGGAGGAGGG CGAGCACGCC TACGACACCC TGAGCCTGGA GAGCTCCGAC 
AGCATGGAGA CCAGCATCTC CACAGGCGGC AACTCAGCCT GCTCGCCCGA CAACATGTCC 
AGTGCGAGCG GCCTGGATGT GGGCAAAATC GAGGAGATGG AGAAGATGCT GAAAGAGGCA 
CACGCCGAGA AGAGCCGGCT CATGGAGTCC AGGGAGCGGG AGATGGAGCT CCGGCGGCAG 
GCCCTGGAGG AGGAACGGAG GAGGCGAGAA CAGGTGGAAC GGAGGCTACA GAGCGAGAGC 
GCCAGGAGGC AGCAGCTGGT GGAGAAGGAG GTCAAGATGC GAGAGAAACA GTTTTCCCAG 
GCACGACCCC TGACCCGCTA CCTGCCAATC CGGAAGGAGG ACTTTGACCT GAAGACCCAC 
ATCGAGTCGT CGGGCCACGG TGTCGATACC TGCCTGCACG TGGTGCTTAG CAGCAAGGTC 
TGCCGCGGCT ACTTGGTCAA GATGGGCGGC AAGATTAAAT CATGGAAGAA GCGCTGGTTT 
GTCTTCGACC GGCTCAAGCG CACCCTTTCC TATTATGTGG ACAAGCACGA GACGAAGCTG 
AAGGGGGTCA TCTACTTCCA GGCCATCGAG GAAGTGTACT ACGATCACCT GCGCAGTGCA 
GCCAAGAGGA GGTTTTTCAG CTTTTCTGTG GTGACTGAGA GCCCGAACCC AGCCCTCACC 
TTCTGTGTGA AGACCCACGA CCGGCTGTAT TACATGGTGG CCCCGTCTGC AGAGGCCATG 
CGCATCTGGA TGGACGTCAT CGTCACGGGA GCGGAGGGCT ACACTCAGTT CATGAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHLDB1 ( XM_014113377.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968852.1 Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.

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CloneID	OCe36311
Clone ID Related Accession (Same CDS sequence)	XM_014113377.1
Accession Version	XM_014113377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pleckstrin homology-like domain family B member 1 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT 
GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG 
CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG 
CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC 
ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC 
ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA 
GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG 
ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG 
CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG 
GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG 
ATGCGAGAGA AACAGTTTTC CCAGCAGGTG GTGTCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968852.1
CDS	426..4667
Translation	MRRPGRGLGWRPGPQELWSPRTMDALNRNQVGPGCKTQAMVKKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLEEGKTVIGSAARDISLQGPGLAPEHCYIENVRGTLTLYPCGNACSIDGLPIRQPTRLTQGCMVCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPGSAESESLVNGNHTPQPATRGPSACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPGAMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPAPSMPPLVPARSSSYHLALQPPQPRPSGARPSESPRLGRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVAISLSEYPASGARSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFRELPSTERVLTTSPSRQLVGRTFSDGSVARTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPMPTRTTPDPKLTREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPRKGSFSGRLSPAYSLGSLTGASPRQSPRAQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQERLWEQEMERLERQRLETILNLCAEYSRADGGLEAGELPSIGEAAAALALAGRRPSRGLSGAPGASGRSTEEPGGATPRLWECVERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEERAQVLGRVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKLVTLETGIQKERDKERAELAAGRRHLEARQALYAELQTQLDNCPESVREQLQEQLRREAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSQAELLRSITKRKERLAVLDSQAGQIRAQAVQESERLARDKNASLQLLQKEKERLTMLEGRYHSLTGGRPFPKTTSTLKEAELLISESSEMGLGSATLVPFPGSSQPGAFSVPLTQPASTQLCPKAQEYVTLEQLKVMWGTSPVPTAPAPGLPFWASASRDLVPTTCLPPVLPSSSSSASLTPSPKMEELLLPAVDLEQWYQELMAGLGTGPAAASPRSSPPPLPAKASRQLQVYRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLAQNGTGSLPRNLAATLQDIETKRQLALQQKGESLPAEPPPADDPAGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPPGPSGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSASGLDVGKIEEMEKMLKEAHAEKSRLMESREREMELRRQALEEERRRREQVERRLQSESARRQQLVEKEVKMREKQFSQQVVSL

Target ORF information:

RefSeq Version	XM_014113377.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pleckstrin homology-like domain, family B, member 1 (PHLDB1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014113377.1

ORF Insert Sequence:

ATGCGACGCC CGGGGCGAGG CCTCGGCTGG CGCCCTGGGC CACAGGAGCT CTGGAGCCCC 
AGGACCATGG ATGCTCTTAA TAGGAACCAG GTAGGCCCTG GATGCAAGAC CCAAGCAATG 
GTAAAGAAAG GGCCCTTGGA CCTGATCGAG ACAGGCAAAG GGCTGAAGGT GCAAACGGAC 
AAACCCCATC TGGTGAGCCT GGGCAGTGGG CGGCTGAGCA CGGCTATCAC CCTTCTGCCT 
CTGGAGGAAG GGAAGACGGT GATTGGCTCC GCAGCCAGGG ACATCTCCCT GCAGGGCCCA 
GGCTTGGCTC CGGAGCACTG CTACATCGAG AACGTGCGGG GCACCCTCAC CCTCTACCCT 
TGTGGCAATG CCTGCAGTAT TGACGGGCTC CCCATCCGGC AGCCCACCAG GCTCACTCAG 
GGCTGTATGG TGTGCCTGGG TCAGTCCACC TTCCTCCGTT TTAACCACCC AGCTGAAGCC 
AAGTGGATGA AGAGCATGAT TCCAGCAGGG GGCCGGGCCC CAGGGCCCCC CTACAGTCCT 
GGCTCAGCAG AATCAGAAAG CCTGGTGAAC GGGAACCACA CCCCACAGCC TGCCACCCGG 
GGACCCTCCG CCTGTGCCAG CCACAGCTCC CTGGTGAGCT CTATTGAGAA GGACCTGCAG 
GAAATCATGG ACTCACTGGT GCTAGAGGAG CCTGGGGCGG CAGGCAAGAA GCCTGCTGCG 
ACTTCCCCAC TGTCGCCGAT GGCTAACGGT GGGCGCTACT TGCTGTCCCC GCCGACCAGC 
CCTGGTGCCA TGTCCGTGGG CTCCAGCTAT GAGAACACCT CTCCAGCCTT CTCTCCACTC 
TCCTCACCAG CCAGCAGTGG AAGCTGTGCC AGCCACTCAC CCAGCGGGCA GGAGCCAGCA 
CCTTCCATGC CCCCGCTGGT CCCTGCCCGT TCCTCCAGCT ACCATCTGGC TCTGCAGCCC 
CCACAGCCCC GACCGAGTGG TGCCCGCCCC TCTGAGAGCC CCCGGTTGGG CAGGAAAGGG 
GGTCACGAGA GGCCTCCCAG TCCTGGCCTC CGAGGTCTGC TGACAGACAG CCCTGCAGCC 
ACGGTCTTGG CTGAGGCCCG CAGAGCCACT GAGAGCCCCC GGCTGGGTGG ACAGCTGCCC 
GTGGTGGCCA TCAGCCTGAG TGAGTACCCG GCTTCCGGTG CCCGAAGCCA ACCCACCAGC 
ATTCCTGGCA GCCCCAAGTT CCAGCCTCCA GTCCCCGCTC CCCGAAACAA GATCGGCACC 
CTCCAGGACC GTCCTCCCAG CCCTTTCCGT GAGCTGCCCA GCACCGAGCG GGTGCTGACA 
ACCAGCCCCT CACGCCAGCT GGTGGGCCGG ACATTTTCAG ATGGGTCAGT CGCCCGCACT 
CTGCAGCCCC CCGAGAGCCC CCGCCTGGGC CGGCGGGGCC TGGACAGTAT GCGGGAATTG 
CCCCCATTGA GCCCGTCACT GTCCCGACGG GCGCTGTCCC CGATGCCCAC CAGGACTACT 
CCAGATCCCA AGCTAACCAG AGAAGTGGCT GAGAGTCCCC GGCCCCGGCG CTGGGCAGCC 
CACGGGGCAT CACCAGAGGA CTTCTCACTG ACCCTGGGGG CACGGGGCCG TAGGACTCGG 
AGTCCCTCAC CCACACTTGG GGAGTCCCTG GCGCCCCGCA AGGGCAGCTT CAGTGGCAGG 
CTGAGCCCGG CCTACAGTCT GGGCTCTCTG ACAGGGGCTT CGCCCCGTCA GAGCCCCCGC 
GCCCAGAGGA AGCTGTCCAG TGGGGACCTG CGGGTACCTG TCACTCGGGA GCGGAAAAAT 
AGCATTACGG AGATCAGCGA CAATGAGGAC GACCTCCTGG AGTACCACCG GCGGCAGCGC 
CAAGAACGGC TCTGGGAGCA GGAGATGGAG AGGCTGGAGC GCCAGCGCCT GGAGACCATA 
CTGAACCTGT GTGCCGAGTA CAGCCGTGCT GATGGGGGCC TCGAGGCGGG GGAGCTGCCT 
AGCATCGGGG AGGCCGCCGC GGCACTGGCG CTGGCGGGCC GCAGGCCCTC ACGAGGCCTT 
TCTGGGGCCC CCGGAGCCTC GGGGCGGAGC ACTGAAGAGC CTGGGGGTGC CACCCCGCGC 
CTGTGGGAGT GTGTGGAGCG CTCAGATGAG GAGAACCTGA AGGAGGAGTG CAGCAGCACG 
GAGAGCACCC AGCAGGAGCA CGAAGATGCC CCTAGCACCA AGCTTCAGGG AGAGGTGCTG 
GCTCTGGAAG AGGAGCGGGC TCAGGTGCTG GGGCGTGTGG AGCAGCTCAA AGTCCGCGTG 
AAGGAGCTGG AGCAGCAGCT GCAGGAGTCA GCCCGAGAGG CTGAGATGGA ACGGGCGTTG 
CTGCAGGGGG AGAGGGAGGC AGAGCGGGCA CTGCTGCAGA AGGAGCAGAA GGCGGTGGAC 
CAGCTGCAGG AGAAGTTAGT GACCTTGGAG ACTGGCATCC AGAAGGAGAG GGACAAGGAG 
AGGGCGGAGC TGGCCGCGGG ACGGAGGCAC CTGGAGGCCC GCCAGGCGCT CTACGCCGAG 
CTCCAGACGC AGCTCGATAA CTGCCCCGAG TCAGTGCGGG AACAGTTACA GGAGCAGCTG 
AGAAGGGAGG CCGAGGCCCT GGAGACGGAG ACAAAGCTGT TTGAGGACTT GGAGTTCCAG 
CAGCTGGAGC GGGAGAGCCG CGTGGAGGAG GAGCGGGAGC TGGCAGGCCA GGGGCTGCTC 
CGGAGCCAGG CCGAGCTGCT CCGCAGCATC ACCAAGAGGA AGGAGCGCCT GGCAGTCCTG 
GACAGTCAGG CGGGGCAGAT CCGGGCCCAG GCCGTGCAGG AGTCCGAGCG CCTGGCGAGG 
GACAAGAACG CTTCCCTGCA GCTGCTGCAG AAGGAGAAGG AGAGACTGAC TATGTTGGAG 
GGAAGATACC ACTCACTCAC AGGAGGCAGG CCCTTCCCGA AGACCACGTC CACCCTCAAA 
GAGGCTGAAC TGCTCATCTC CGAGTCCTCA GAGATGGGGC TGGGGTCGGC GACTCTGGTC 
CCTTTCCCAG GGTCTTCTCA GCCCGGGGCC TTCTCTGTCC CCCTAACCCA ACCTGCTTCC 
ACTCAGCTCT GCCCCAAAGC ACAAGAGTAC GTGACGCTTG AGCAGCTAAA GGTGATGTGG 
GGCACCTCAC CCGTGCCCAC AGCCCCCGCG CCGGGCCTGC CTTTCTGGGC CTCTGCCTCC 
CGGGACCTGG TTCCCACCAC CTGCCTTCCT CCCGTGCTGC CCTCTTCCTC CTCCTCCGCT 
TCTCTCACGC CTTCACCCAA GATGGAGGAG CTGCTACTCC CTGCTGTAGA CTTAGAGCAG 
TGGTACCAGG AGCTGATGGC CGGGCTGGGG ACCGGCCCCG CTGCAGCCTC CCCGCGCTCC 
TCCCCCCCGC CTCTGCCCGC CAAAGCTTCC CGGCAGCTGC AGGTTTACCG CTCCAAGATG 
GATGGTGAGG CCACCAGCCC CCTGCCCCGG ACCCGCAGTG GCCCCCTCCC CTCCTCCTCT 
GGTTCTTCTT CCTCTTCTTC GCAGCTCAGC GTGGCTACTC TGGGCCGGAG CCCCTCCCCA 
AAGAGTGCCC TACTTGCCCA GAATGGCACA GGCAGCCTTC CTCGCAACCT GGCGGCCACG 
CTGCAGGACA TTGAGACCAA GCGCCAGCTG GCCCTGCAGC AGAAGGGCGA GTCGCTTCCT 
GCAGAGCCCC CCCCAGCAGA CGACCCAGCA GGACAGCAGG TGATCGAGGA GCAGCGGCGG 
CGCCTGGCCG AGCTGAAGCA GAAGGCTGCG GCCGAGGCGC AGTGCCAGTG GGACGCCCTG 
CACGGGGCGG CCCCCTTCCC CCCGGGCCCC TCGGGCTTCC CCCCGCTCAT GCACCACTCC 
ATCCTGCACC ACCTGCCGGC TGGCCGGGAG CGCGGGGAGG AGGGCGAGCA CGCCTACGAC 
ACCCTGAGCC TGGAGAGCTC CGACAGCATG GAGACCAGCA TCTCCACAGG CGGCAACTCA 
GCCTGCTCGC CCGACAACAT GTCCAGTGCG AGCGGCCTGG ATGTGGGCAA AATCGAGGAG 
ATGGAGAAGA TGCTGAAAGA GGCACACGCC GAGAAGAGCC GGCTCATGGA GTCCAGGGAG 
CGGGAGATGG AGCTCCGGCG GCAGGCCCTG GAGGAGGAAC GGAGGAGGCG AGAACAGGTG 
GAACGGAGGC TACAGAGCGA GAGCGCCAGG AGGCAGCAGC TGGTGGAGAA GGAGGTCAAG 
ATGCGAGAGA AACAGTTTTC CCAGCAGGTG GTGTCGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHLDB1
Entrez Gene ID	489381
Full Name	pleckstrin homology like domain family B member 1
General protein information	Preferred Names pleckstrin homology like domain family B member 1 Names pleckstrin homology-like domain family B member 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	PHLDB1	NP_001138231.1
Gallus gallus (chicken)	PHLDB1	XP_417927.4
Danio rerio (zebrafish)	LOC570275	XP_005157508.1
Xenopus tropicalis (tropical clawed frog)	phldb1	NP_001123804.1
Canis lupus familiaris (dog)	PHLDB1	XP_005619762.1
Bos taurus (cattle)	PHLDB1	NP_001192971.1
Rattus norvegicus (Norway rat)	Phldb1	NP_001178507.1
Pan troglodytes (chimpanzee)	PHLDB1	XP_001162204.1
Mus musculus (house mouse)	Phldb1	NP_705765.3
	LOC706336	XP_002808134.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHLDB1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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