LETM1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LETM1
Entrez Gene ID	488807
Full Name	leucine zipper and EF-hand containing transmembrane protein 1
General protein information	Preferred Names leucine zipper and EF-hand containing transmembrane protein 1 Names LETM1 and EF-hand domain-containing protein 1, mitochondrial mitochondrial proton/calcium exchanger protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022417132.1	XP_022272840.1	mitochondrial proton/calcium exchanger protein isoform X2
XM_022417133.1	XP_022272841.1	mitochondrial proton/calcium exchanger protein isoform X3
XM_005618526.2	XP_005618583.1	LETM1 and EF-hand domain-containing protein 1, mitochondrial
XM_005618526.3	XP_005618583.1	mitochondrial proton/calcium exchanger protein isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LETM1	NP_001069082.1
Mus musculus (house mouse)	Letm1	NP_062668.1
Danio rerio (zebrafish)	letm1	NP_001038673.1
Kluyveromyces lactis	KLLA0B11341g	XP_452034.1
Neurospora crassa	NCU03217	XP_965317.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003296	XP_003436436.1
Caenorhabditis elegans (roundworm)	letm-1	NP_506381.1
Saccharomyces cerevisiae (baker's yeast)	YLH47	NP_015450.1
Eremothecium gossypii	AGOS_AAR032W	NP_982573.2
Xenopus tropicalis (tropical clawed frog)	letm1	NP_001072793.1
Canis lupus familiaris (dog)	LETM1	XP_545925.2
Rattus norvegicus (Norway rat)	Letm1	NP_001005884.1
Magnaporthe oryzae (rice blast fungus)	MGG_03684	XP_003716197.1
Homo sapiens (human)	LETM1	NP_036450.1
Gallus gallus (chicken)	LETM1	NP_001006461.2
Pan troglodytes (chimpanzee)	LETM1	XP_003310276.1
	LETM1	XP_002802216.1
Schizosaccharomyces pombe (fission yeast)	mdm28	NP_593648.1

Gene Ontology

The following LETM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LETM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe04474	XM_005618526.3 Latest version!	Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe287353	XM_022417132.1 Latest version!	Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe287354	XM_022417133.1 Latest version!	Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OCe04474	XM_005618526.2	Canis lupus familiaris leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LETM1 ( XM_005618526.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618583.1 Canis lupus familiaris leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), mRNA.

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CloneID	OCe04474
Clone ID Related Accession (Same CDS sequence)	XM_005618526.2 , XM_005618526.3
Accession Version	XM_005618526.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	LETM1 and EF-hand domain-containing protein 1, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGAAGG AGGTGGAGCC AGACGTGGTG 
GCAGAAGCTG CCCCCGGGAG GTCTGTGGCC AAGCTGCAGC CAGAAGTGCC TGAGGTGACC 
CTGCCGTCAG AGGCCCTGCA GGACACTGCC CCCGTCCTGG AAGGCTTGAA GGAAGAGGAA 
ATAACAAAAG AGGAGATTGA CATACTGAGC AACGCCTGCT CGAAGCTGAA GGAGCAGAAG 
TCGTCCCTGA CGAAGGAGAA GGAGGAGCTG GAGCTACTCA AGGAGGACGT GCAGGACTAC 
AGCGAGGACT TGCAAGAGAT CAAGAAGGAA CTTTCAAAGA CTGGCGAAGA GATCTATGTG 
GAGGAGTCTA AAGCCAGCAA GAGGCTGACG AAGAGGGTGC AGCAGATGAT CGGGCAGATA 
GATAGCCTGC TCTCACAGCT GGAGATGGAC CAGAAGGCCG GCAAGTTGGG CCCTGCCGCC 
GAGAGCCCAC CTGTGGGAGA CAATGTCATC AGCATCAATG AGCTCATCAG TGCCATGAAG 
CAAATCAAGC ACATTCCAGA AAACAAGCTC ATGAGTTTGG CCTCAGCTCT GGATGAAAAC 
AAGGACGGCA AGATCAACAT TGATGACCTC GTCAAGGTGA TTGAGCTGGT GGACAAGGAA 
GATGTCCACA TCTCCACCAG CCAGGTGGCT GAGATCGTGG CCCTGCTGGA AAAGGAAGAG 
AAGGTGGAGG AGAAGGAGAA GGCGAAGGAG AAGGCGGAGA AGGAGGCTGC CGAAGTAAAG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618583.1
CDS	231..2456
Translation	MASILLRSCRGRGPARLPPPRAAAGRGGLGDGACLGCASTVGLISHLNVPFHCCTPVHPIHLAFRANPRGCWTVRPERVCVAAGVPWTPTSANLLIPGLQFLPLRCWHSSPPLRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPVEVAVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILHGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSVFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQSACRARGMRALGVTEDRLRDQLKQWLELHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQRLSEAAVKEVEPDVVAEAAPGRSVAKLQPEVPEVTLPSEALQDTAPVLEGLKEEEITKEEIDILSNACSKLKEQKSSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEIYVEESKASKRLTKRVQQMIGQIDSLLSQLEMDQKAGKLGPAAESPPVGDNVISINELISAMKQIKHIPENKLMSLASALDENKDGKINIDDLVKVIELVDKEDVHISTSQVAEIVALLEKEEKVEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	XM_005618526.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618526.2

ORF Insert Sequence:

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGAAGG AGGTGGAGCC AGACGTGGTG 
GCAGAAGCTG CCCCCGGGAG GTCTGTGGCC AAGCTGCAGC CAGAAGTGCC TGAGGTGACC 
CTGCCGTCAG AGGCCCTGCA GGACACTGCC CCCGTCCTGG AAGGCTTGAA GGAAGAGGAA 
ATAACAAAAG AGGAGATTGA CATACTGAGC AACGCCTGCT CGAAGCTGAA GGAGCAGAAG 
TCGTCCCTGA CGAAGGAGAA GGAGGAGCTG GAGCTACTCA AGGAGGACGT GCAGGACTAC 
AGCGAGGACT TGCAAGAGAT CAAGAAGGAA CTTTCAAAGA CTGGCGAAGA GATCTATGTG 
GAGGAGTCTA AAGCCAGCAA GAGGCTGACG AAGAGGGTGC AGCAGATGAT CGGGCAGATA 
GATAGCCTGC TCTCACAGCT GGAGATGGAC CAGAAGGCCG GCAAGTTGGG CCCTGCCGCC 
GAGAGCCCAC CTGTGGGAGA CAATGTCATC AGCATCAATG AGCTCATCAG TGCCATGAAG 
CAAATCAAGC ACATTCCAGA AAACAAGCTC ATGAGTTTGG CCTCAGCTCT GGATGAAAAC 
AAGGACGGCA AGATCAACAT TGATGACCTC GTCAAGGTGA TTGAGCTGGT GGACAAGGAA 
GATGTCCACA TCTCCACCAG CCAGGTGGCT GAGATCGTGG CCCTGCTGGA AAAGGAAGAG 
AAGGTGGAGG AGAAGGAGAA GGCGAAGGAG AAGGCGGAGA AGGAGGCTGC CGAAGTAAAG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LETM1 ( XM_005618526.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618583.1 Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe04474
Clone ID Related Accession (Same CDS sequence)	XM_005618526.2 , XM_005618526.3
Accession Version	XM_005618526.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitochondrial proton/calcium exchanger protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGAAGG AGGTGGAGCC AGACGTGGTG 
GCAGAAGCTG CCCCCGGGAG GTCTGTGGCC AAGCTGCAGC CAGAAGTGCC TGAGGTGACC 
CTGCCGTCAG AGGCCCTGCA GGACACTGCC CCCGTCCTGG AAGGCTTGAA GGAAGAGGAA 
ATAACAAAAG AGGAGATTGA CATACTGAGC AACGCCTGCT CGAAGCTGAA GGAGCAGAAG 
TCGTCCCTGA CGAAGGAGAA GGAGGAGCTG GAGCTACTCA AGGAGGACGT GCAGGACTAC 
AGCGAGGACT TGCAAGAGAT CAAGAAGGAA CTTTCAAAGA CTGGCGAAGA GATCTATGTG 
GAGGAGTCTA AAGCCAGCAA GAGGCTGACG AAGAGGGTGC AGCAGATGAT CGGGCAGATA 
GATAGCCTGC TCTCACAGCT GGAGATGGAC CAGAAGGCCG GCAAGTTGGG CCCTGCCGCC 
GAGAGCCCAC CTGTGGGAGA CAATGTCATC AGCATCAATG AGCTCATCAG TGCCATGAAG 
CAAATCAAGC ACATTCCAGA AAACAAGCTC ATGAGTTTGG CCTCAGCTCT GGATGAAAAC 
AAGGACGGCA AGATCAACAT TGATGACCTC GTCAAGGTGA TTGAGCTGGT GGACAAGGAA 
GATGTCCACA TCTCCACCAG CCAGGTGGCT GAGATCGTGG CCCTGCTGGA AAAGGAAGAG 
AAGGTGGAGG AGAAGGAGAA GGCGAAGGAG AAGGCGGAGA AGGAGGCTGC CGAAGTAAAG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618583.1
CDS	52..2277
Translation	MASILLRSCRGRGPARLPPPRAAAGRGGLGDGACLGCASTVGLISHLNVPFHCCTPVHPIHLAFRANPRGCWTVRPERVCVAAGVPWTPTSANLLIPGLQFLPLRCWHSSPPLRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPVEVAVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILHGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSVFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQSACRARGMRALGVTEDRLRDQLKQWLELHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQRLSEAAVKEVEPDVVAEAAPGRSVAKLQPEVPEVTLPSEALQDTAPVLEGLKEEEITKEEIDILSNACSKLKEQKSSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEIYVEESKASKRLTKRVQQMIGQIDSLLSQLEMDQKAGKLGPAAESPPVGDNVISINELISAMKQIKHIPENKLMSLASALDENKDGKINIDDLVKVIELVDKEDVHISTSQVAEIVALLEKEEKVEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	XM_005618526.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618526.3

ORF Insert Sequence:

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGAAGG AGGTGGAGCC AGACGTGGTG 
GCAGAAGCTG CCCCCGGGAG GTCTGTGGCC AAGCTGCAGC CAGAAGTGCC TGAGGTGACC 
CTGCCGTCAG AGGCCCTGCA GGACACTGCC CCCGTCCTGG AAGGCTTGAA GGAAGAGGAA 
ATAACAAAAG AGGAGATTGA CATACTGAGC AACGCCTGCT CGAAGCTGAA GGAGCAGAAG 
TCGTCCCTGA CGAAGGAGAA GGAGGAGCTG GAGCTACTCA AGGAGGACGT GCAGGACTAC 
AGCGAGGACT TGCAAGAGAT CAAGAAGGAA CTTTCAAAGA CTGGCGAAGA GATCTATGTG 
GAGGAGTCTA AAGCCAGCAA GAGGCTGACG AAGAGGGTGC AGCAGATGAT CGGGCAGATA 
GATAGCCTGC TCTCACAGCT GGAGATGGAC CAGAAGGCCG GCAAGTTGGG CCCTGCCGCC 
GAGAGCCCAC CTGTGGGAGA CAATGTCATC AGCATCAATG AGCTCATCAG TGCCATGAAG 
CAAATCAAGC ACATTCCAGA AAACAAGCTC ATGAGTTTGG CCTCAGCTCT GGATGAAAAC 
AAGGACGGCA AGATCAACAT TGATGACCTC GTCAAGGTGA TTGAGCTGGT GGACAAGGAA 
GATGTCCACA TCTCCACCAG CCAGGTGGCT GAGATCGTGG CCCTGCTGGA AAAGGAAGAG 
AAGGTGGAGG AGAAGGAGAA GGCGAAGGAG AAGGCGGAGA AGGAGGCTGC CGAAGTAAAG 
AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LETM1 ( XM_022417132.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272840.1 Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X2, mRNA.

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CloneID	OCe287353
Clone ID Related Accession (Same CDS sequence)	XM_022417132.1
Accession Version	XM_022417132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitochondrial proton/calcium exchanger protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGGAGG TGGAGCCAGA CGTGGTGGCA 
GAAGCTGCCC CCGGGAGGTC TGTGGCCAAG CTGCAGCCAG AAGTGCCTGA GGTGACCCTG 
CCGTCAGAGG CCCTGCAGGA CACTGCCCCC GTCCTGGAAG GCTTGAAGGA AGAGGAAATA 
ACAAAAGAGG AGATTGACAT ACTGAGCAAC GCCTGCTCGA AGCTGAAGGA GCAGAAGTCG 
TCCCTGACGA AGGAGAAGGA GGAGCTGGAG CTACTCAAGG AGGACGTGCA GGACTACAGC 
GAGGACTTGC AAGAGATCAA GAAGGAACTT TCAAAGACTG GCGAAGAGAT CTATGTGGAG 
GAGTCTAAAG CCAGCAAGAG GCTGACGAAG AGGGTGCAGC AGATGATCGG GCAGATAGAT 
AGCCTGCTCT CACAGCTGGA GATGGACCAG AAGGCCGGCA AGTTGGGCCC TGCCGCCGAG 
AGCCCACCTG TGGGAGACAA TGTCATCAGC ATCAATGAGC TCATCAGTGC CATGAAGCAA 
ATCAAGCACA TTCCAGAAAA CAAGCTCATG AGTTTGGCCT CAGCTCTGGA TGAAAACAAG 
GACGGCAAGA TCAACATTGA TGACCTCGTC AAGGTGATTG AGCTGGTGGA CAAGGAAGAT 
GTCCACATCT CCACCAGCCA GGTGGCTGAG ATCGTGGCCC TGCTGGAAAA GGAAGAGAAG 
GTGGAGGAGA AGGAGAAGGC GAAGGAGAAG GCGGAGAAGG AGGCTGCCGA AGTAAAGAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272840.1
CDS	52..2274
Translation	MASILLRSCRGRGPARLPPPRAAAGRGGLGDGACLGCASTVGLISHLNVPFHCCTPVHPIHLAFRANPRGCWTVRPERVCVAAGVPWTPTSANLLIPGLQFLPLRCWHSSPPLRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPVEVAVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILHGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSVFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQSACRARGMRALGVTEDRLRDQLKQWLELHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQRLSEAAVEVEPDVVAEAAPGRSVAKLQPEVPEVTLPSEALQDTAPVLEGLKEEEITKEEIDILSNACSKLKEQKSSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEIYVEESKASKRLTKRVQQMIGQIDSLLSQLEMDQKAGKLGPAAESPPVGDNVISINELISAMKQIKHIPENKLMSLASALDENKDGKINIDDLVKVIELVDKEDVHISTSQVAEIVALLEKEEKVEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	XM_022417132.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417132.1

ORF Insert Sequence:

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGGAGG TGGAGCCAGA CGTGGTGGCA 
GAAGCTGCCC CCGGGAGGTC TGTGGCCAAG CTGCAGCCAG AAGTGCCTGA GGTGACCCTG 
CCGTCAGAGG CCCTGCAGGA CACTGCCCCC GTCCTGGAAG GCTTGAAGGA AGAGGAAATA 
ACAAAAGAGG AGATTGACAT ACTGAGCAAC GCCTGCTCGA AGCTGAAGGA GCAGAAGTCG 
TCCCTGACGA AGGAGAAGGA GGAGCTGGAG CTACTCAAGG AGGACGTGCA GGACTACAGC 
GAGGACTTGC AAGAGATCAA GAAGGAACTT TCAAAGACTG GCGAAGAGAT CTATGTGGAG 
GAGTCTAAAG CCAGCAAGAG GCTGACGAAG AGGGTGCAGC AGATGATCGG GCAGATAGAT 
AGCCTGCTCT CACAGCTGGA GATGGACCAG AAGGCCGGCA AGTTGGGCCC TGCCGCCGAG 
AGCCCACCTG TGGGAGACAA TGTCATCAGC ATCAATGAGC TCATCAGTGC CATGAAGCAA 
ATCAAGCACA TTCCAGAAAA CAAGCTCATG AGTTTGGCCT CAGCTCTGGA TGAAAACAAG 
GACGGCAAGA TCAACATTGA TGACCTCGTC AAGGTGATTG AGCTGGTGGA CAAGGAAGAT 
GTCCACATCT CCACCAGCCA GGTGGCTGAG ATCGTGGCCC TGCTGGAAAA GGAAGAGAAG 
GTGGAGGAGA AGGAGAAGGC GAAGGAGAAG GCGGAGAAGG AGGCTGCCGA AGTAAAGAAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LETM1 ( XM_022417133.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272841.1 Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X3, mRNA.

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CloneID	OCe287354
Clone ID Related Accession (Same CDS sequence)	XM_022417133.1
Accession Version	XM_022417133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mitochondrial proton/calcium exchanger protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGAAGG AGGTGGAGCC AGACGTGGTG 
GCAGAAGCTG CCCCCGGGAG GTCTGTGGCC AAGCTGCAGC CAGAAGTGCC TGAGGTGACC 
CTGCCGTCAG AGGCCCTGCA GGACACTGCC CCCGTCCTGG AAGGCTTGAA GGAAGAGGAA 
ATAACAAAAG AGGAGATTGA CATACTGAGC AACGCCTGCT CGAAGCTGAA GGAGCAGAAG 
TCGTCCCTGA CGAAGGAGAA GGAGGAGCTG GAGCTACTCA AGGAGGACGT GCAGGACTAC 
AGCGAGGACT TGCAAGAGAT CAAGAAGGAA CTTTCAAAGA CTGGCGAAGA GATCTATGTG 
GAGGAGTCTA AAGCCAGCAA GAGGCTGACG AAGAGGGTGC AGCAGATGAT CGGGCAGATA 
GATAGCCTGC TCTCACAGCT GGAGATGGAC CAGAAGGCCG GCAAGTTGGG CCCTGCCGCC 
GAGAGCCCAC CTGTGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272841.1
CDS	52..1995
Translation	MASILLRSCRGRGPARLPPPRAAAGRGGLGDGACLGCASTVGLISHLNVPFHCCTPVHPIHLAFRANPRGCWTVRPERVCVAAGVPWTPTSANLLIPGLQFLPLRCWHSSPPLRDDSVVEKSLKSLKDKNKKLEEGGPVYSPPVEVAVKKSLGQRVLDELKHYYHGFRLLWIDTKIAARMLWRILHGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVAVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSVFFQKIRETGERPSNEEILRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLIAEEGVDSLNVKELQSACRARGMRALGVTEDRLRDQLKQWLELHLHQEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEQVDNKAKLEATLQEEAAIQQEHREKELQRLSEAAVKEVEPDVVAEAAPGRSVAKLQPEVPEVTLPSEALQDTAPVLEGLKEEEITKEEIDILSNACSKLKEQKSSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEIYVEESKASKRLTKRVQQMIGQIDSLLSQLEMDQKAGKLGPAAESPPVGS

Target ORF information:

RefSeq Version	XM_022417133.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris leucine zipper and EF-hand containing transmembrane protein 1 (LETM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417133.1

ORF Insert Sequence:

ATGGCGTCCA TCTTACTGAG GAGCTGCCGC GGCCGGGGGC CCGCTCGCCT CCCGCCGCCC 
CGTGCCGCAG CCGGGAGAGG TGGTCTGGGA GATGGTGCTT GTCTTGGCTG TGCCAGCACC 
GTGGGGTTGA TAAGCCACTT GAATGTTCCA TTTCACTGCT GCACTCCCGT CCACCCCATC 
CACCTTGCCT TTAGAGCCAA TCCTCGTGGC TGTTGGACTG TGAGGCCTGA GCGCGTGTGC 
GTGGCAGCCG GAGTGCCCTG GACCCCCACC TCGGCCAACT TGCTCATCCC AGGGCTTCAG 
TTCCTTCCTT TGCGCTGCTG GCACTCATCA CCCCCACTGA GGGATGACTC CGTGGTGGAG 
AAGTCGCTCA AGTCCTTAAA GGACAAGAAC AAGAAGCTGG AGGAGGGAGG CCCTGTGTAC 
AGCCCCCCTG TGGAGGTGGC TGTGAAGAAG TCCTTGGGGC AGCGGGTCTT GGACGAGCTG 
AAGCACTATT ATCACGGCTT CCGCCTGCTC TGGATTGACA CCAAGATCGC CGCGCGCATG 
CTCTGGCGCA TCCTCCACGG CCACACTCTG ACCCGCAGGG AGCGCAGGCA GTTTCTCCGG 
ATATGCGCTG ACCTCTTCCG CCTCGTCCCC TTCCTGGTCT TTGTGGTGGT GCCTTTCATG 
GAGTTTCTGC TTCCTGTCGC TGTGAAGCTC TTCCCCAATA TGTTGCCATC CACGTTCGAG 
ACCCAGTCCA TCAAGGAGGA GAGGCTGAAG AAGGAGCTGC GGGTCAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT TGAAGAGATG GCCCTGAAGA ACAAAGCAGC CAAGGGGAAT 
GCCACCAAAG ACTTCTCTGT GTTTTTCCAG AAGATCCGAG AGACAGGTGA GAGACCCAGT 
AATGAGGAGA TCCTGCGTTT TTCCAAGTTG TTTGAAGATG AGCTGACCCT GGATAACCTC 
ACACGGCCTC AGCTGGTGGC CCTCTGCAAG CTGCTGGAGC TCCAGTCCAT TGGCACCAAC 
AACTTCCTCC GCTTCCAGCT CACCATGAGG CTGCGGTCTA TCAAGGCTGA CGACAAGTTG 
ATTGCTGAGG AAGGGGTGGA CAGCCTGAAT GTCAAGGAAT TGCAGTCAGC ATGTCGGGCA 
CGAGGCATGC GGGCCCTTGG CGTCACGGAG GATCGTCTGA GGGACCAACT GAAGCAGTGG 
CTGGAGTTGC ACCTGCATCA GGAGATCCCA ACGTCGCTGC TCATCCTGTC CCGAGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCCGAC CAGCTCAAGT CCACGCTGCA GACACTCCCA 
GAGATCGTGG CAAAGGAAGC ACAGGTGAAA GTGGCTGAAG TGGAAGGGGA GCAGGTGGAC 
AACAAGGCAA AGCTGGAGGC CACGCTGCAG GAGGAGGCGG CCATTCAGCA GGAACACCGG 
GAGAAGGAGC TGCAGAGGCT CTCTGAGGCG GCAGTGAAGG AGGTGGAGCC AGACGTGGTG 
GCAGAAGCTG CCCCCGGGAG GTCTGTGGCC AAGCTGCAGC CAGAAGTGCC TGAGGTGACC 
CTGCCGTCAG AGGCCCTGCA GGACACTGCC CCCGTCCTGG AAGGCTTGAA GGAAGAGGAA 
ATAACAAAAG AGGAGATTGA CATACTGAGC AACGCCTGCT CGAAGCTGAA GGAGCAGAAG 
TCGTCCCTGA CGAAGGAGAA GGAGGAGCTG GAGCTACTCA AGGAGGACGT GCAGGACTAC 
AGCGAGGACT TGCAAGAGAT CAAGAAGGAA CTTTCAAAGA CTGGCGAAGA GATCTATGTG 
GAGGAGTCTA AAGCCAGCAA GAGGCTGACG AAGAGGGTGC AGCAGATGAT CGGGCAGATA 
GATAGCCTGC TCTCACAGCT GGAGATGGAC CAGAAGGCCG GCAAGTTGGG CCCTGCCGCC 
GAGAGCCCAC CTGTGGGCTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LETM1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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