TTLL4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TTLL4
Entrez Gene ID	488525
Full Name	tubulin tyrosine ligase like 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005640665.2	XP_005640722.1	tubulin polyglutamylase TTLL4
XM_545645.5	XP_545645.2	tubulin polyglutamylase TTLL4
XM_005640664.2	XP_005640721.1	tubulin polyglutamylase TTLL4
XM_005640664.3	XP_005640721.1	tubulin polyglutamylase TTLL4
XM_005640665.3	XP_005640722.1	tubulin polyglutamylase TTLL4
XM_545645.6	XP_545645.2	tubulin polyglutamylase TTLL4

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	ttll-4	NP_001022985.1
Rattus norvegicus (Norway rat)	Ttll4	XP_006245360.1
Homo sapiens (human)	TTLL4	NP_055455.3
Pan troglodytes (chimpanzee)	TTLL4	XP_001159133.1
	TTLL4	XP_001094864.1
Bos taurus (cattle)	TTLL4	NP_001192637.1
Mus musculus (house mouse)	Ttll4	NP_001014974.1
Xenopus tropicalis (tropical clawed frog)	ttll4	XP_002933990.2
Canis lupus familiaris (dog)	TTLL4	XP_545645.2
Gallus gallus (chicken)	TTLL4	XP_422058.4

Gene Ontology

The following TTLL4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTLL4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe31507	XM_005640664.3 Latest version!	Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe31507	XM_005640665.3 Latest version!	Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe31507	XM_545645.6 Latest version!	Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OCe31507	XM_005640664.2	Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe31507	XM_005640665.2	Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe31507	XM_545645.5	Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTLL4 ( XM_005640665.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640722.1 Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X3, mRNA.

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CloneID	OCe31507
Clone ID Related Accession (Same CDS sequence)	XM_005640665.2 , XM_545645.5 , XM_005640664.2 , XM_005640664.3 , XM_005640665.3 , XM_545645.6
Accession Version	XM_005640665.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tubulin polyglutamylase TTLL4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640722.1
CDS	515..4117
Translation	MASARTEHYDIGLRRGNSLKQSGPSDTAPAPPPEKASEGRAWAQAHQQVKPIWKLERKHVGTLSAGLGPSVLGVPPQPAYFFCPGTLCSSGHSAVISGHGSSCYLRSLPDLFSSTLLYRRSSHRHKPYQQLESFCLRSSLPEKRPFSLPHKGLPVRLTANKAPSFTVSPMAQPMASSSTDRYLSLGAAGENPSGKCLAAAISGKIPSPLSSSYKPMLNNNSFMRPNSTKVPLSQATEGLKPVSSPKIHLVSWHHSGGTGDCALQPVEHKVPKSHGAVLDDAPAPGTLSAPSSLDTLTTSVASPQYNRSNLATRAEPHPCGLDGNFVSQALTREVRFTEAVRKLAARGFEKKPRQGCQLEQSRFMNPSLQCELLNRNRQWKPPTVGQQFPQEDAGANSRTLPSPSDALELDGTVFCTKRISIHLLASHASALSCSPACGSAIDSPPPGEDKTPEPPAPPQPLGVADVATRLSSIHLGQLGKEGPTEARGPDFPARDISSAADLQPDLGEAEDLEEELVDGLEDCCSHDENEEEEGDSECSSFSAVSTGESVAVISRSCAEILTKPLSSEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDAKLLRKAWESGSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNAEYQANADETACQGHKWALKALWNYLSQKGVNSDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPTAEDIVSSSGSSSSSSASLPSSPKDKCRMAPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHTGAICDSAPMWSLPASLLTIPKGDVGLSTFPKLESGKLGKHSFSEGSIPLCEDGTMPKPKKTQAGLCPVPGKPSSSKDSEDISKEPSLSTQMLPLLKYSGQTSRLSASPTSQSTGDSLLAAVSP

Target ORF information:

RefSeq Version	XM_005640665.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640665.2

ORF Insert Sequence:

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4 ( XM_545645.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545645.2 Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe31507
Clone ID Related Accession (Same CDS sequence)	XM_005640665.2 , XM_545645.5 , XM_005640664.2 , XM_005640664.3 , XM_005640665.3 , XM_545645.6
Accession Version	XM_545645.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tubulin polyglutamylase TTLL4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_545645.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545645.2
CDS	421..4023
Translation	MASARTEHYDIGLRRGNSLKQSGPSDTAPAPPPEKASEGRAWAQAHQQVKPIWKLERKHVGTLSAGLGPSVLGVPPQPAYFFCPGTLCSSGHSAVISGHGSSCYLRSLPDLFSSTLLYRRSSHRHKPYQQLESFCLRSSLPEKRPFSLPHKGLPVRLTANKAPSFTVSPMAQPMASSSTDRYLSLGAAGENPSGKCLAAAISGKIPSPLSSSYKPMLNNNSFMRPNSTKVPLSQATEGLKPVSSPKIHLVSWHHSGGTGDCALQPVEHKVPKSHGAVLDDAPAPGTLSAPSSLDTLTTSVASPQYNRSNLATRAEPHPCGLDGNFVSQALTREVRFTEAVRKLAARGFEKKPRQGCQLEQSRFMNPSLQCELLNRNRQWKPPTVGQQFPQEDAGANSRTLPSPSDALELDGTVFCTKRISIHLLASHASALSCSPACGSAIDSPPPGEDKTPEPPAPPQPLGVADVATRLSSIHLGQLGKEGPTEARGPDFPARDISSAADLQPDLGEAEDLEEELVDGLEDCCSHDENEEEEGDSECSSFSAVSTGESVAVISRSCAEILTKPLSSEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDAKLLRKAWESGSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNAEYQANADETACQGHKWALKALWNYLSQKGVNSDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPTAEDIVSSSGSSSSSSASLPSSPKDKCRMAPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHTGAICDSAPMWSLPASLLTIPKGDVGLSTFPKLESGKLGKHSFSEGSIPLCEDGTMPKPKKTQAGLCPVPGKPSSSKDSEDISKEPSLSTQMLPLLKYSGQTSRLSASPTSQSTGDSLLAAVSP

Target ORF information:

RefSeq Version	XM_545645.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545645.5

ORF Insert Sequence:

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4 ( XM_005640664.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640721.1 Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X2, mRNA.

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CloneID	OCe31507
Clone ID Related Accession (Same CDS sequence)	XM_005640665.2 , XM_545645.5 , XM_005640664.2 , XM_005640664.3 , XM_005640665.3 , XM_545645.6
Accession Version	XM_005640664.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	tubulin polyglutamylase TTLL4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640721.1
CDS	613..4215
Translation	MASARTEHYDIGLRRGNSLKQSGPSDTAPAPPPEKASEGRAWAQAHQQVKPIWKLERKHVGTLSAGLGPSVLGVPPQPAYFFCPGTLCSSGHSAVISGHGSSCYLRSLPDLFSSTLLYRRSSHRHKPYQQLESFCLRSSLPEKRPFSLPHKGLPVRLTANKAPSFTVSPMAQPMASSSTDRYLSLGAAGENPSGKCLAAAISGKIPSPLSSSYKPMLNNNSFMRPNSTKVPLSQATEGLKPVSSPKIHLVSWHHSGGTGDCALQPVEHKVPKSHGAVLDDAPAPGTLSAPSSLDTLTTSVASPQYNRSNLATRAEPHPCGLDGNFVSQALTREVRFTEAVRKLAARGFEKKPRQGCQLEQSRFMNPSLQCELLNRNRQWKPPTVGQQFPQEDAGANSRTLPSPSDALELDGTVFCTKRISIHLLASHASALSCSPACGSAIDSPPPGEDKTPEPPAPPQPLGVADVATRLSSIHLGQLGKEGPTEARGPDFPARDISSAADLQPDLGEAEDLEEELVDGLEDCCSHDENEEEEGDSECSSFSAVSTGESVAVISRSCAEILTKPLSSEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDAKLLRKAWESGSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNAEYQANADETACQGHKWALKALWNYLSQKGVNSDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPTAEDIVSSSGSSSSSSASLPSSPKDKCRMAPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHTGAICDSAPMWSLPASLLTIPKGDVGLSTFPKLESGKLGKHSFSEGSIPLCEDGTMPKPKKTQAGLCPVPGKPSSSKDSEDISKEPSLSTQMLPLLKYSGQTSRLSASPTSQSTGDSLLAAVSP

Target ORF information:

RefSeq Version	XM_005640664.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris tubulin tyrosine ligase-like family member 4 (TTLL4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640664.2

ORF Insert Sequence:

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4 ( XM_005640664.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640721.1 Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X2, mRNA.

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CloneID	OCe31507
Clone ID Related Accession (Same CDS sequence)	XM_005640665.2 , XM_545645.5 , XM_005640664.2 , XM_005640664.3 , XM_005640665.3 , XM_545645.6
Accession Version	XM_005640664.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tubulin polyglutamylase TTLL4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640721.1
CDS	391..3993
Translation	MASARTEHYDIGLRRGNSLKQSGPSDTAPAPPPEKASEGRAWAQAHQQVKPIWKLERKHVGTLSAGLGPSVLGVPPQPAYFFCPGTLCSSGHSAVISGHGSSCYLRSLPDLFSSTLLYRRSSHRHKPYQQLESFCLRSSLPEKRPFSLPHKGLPVRLTANKAPSFTVSPMAQPMASSSTDRYLSLGAAGENPSGKCLAAAISGKIPSPLSSSYKPMLNNNSFMRPNSTKVPLSQATEGLKPVSSPKIHLVSWHHSGGTGDCALQPVEHKVPKSHGAVLDDAPAPGTLSAPSSLDTLTTSVASPQYNRSNLATRAEPHPCGLDGNFVSQALTREVRFTEAVRKLAARGFEKKPRQGCQLEQSRFMNPSLQCELLNRNRQWKPPTVGQQFPQEDAGANSRTLPSPSDALELDGTVFCTKRISIHLLASHASALSCSPACGSAIDSPPPGEDKTPEPPAPPQPLGVADVATRLSSIHLGQLGKEGPTEARGPDFPARDISSAADLQPDLGEAEDLEEELVDGLEDCCSHDENEEEEGDSECSSFSAVSTGESVAVISRSCAEILTKPLSSEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDAKLLRKAWESGSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNAEYQANADETACQGHKWALKALWNYLSQKGVNSDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPTAEDIVSSSGSSSSSSASLPSSPKDKCRMAPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHTGAICDSAPMWSLPASLLTIPKGDVGLSTFPKLESGKLGKHSFSEGSIPLCEDGTMPKPKKTQAGLCPVPGKPSSSKDSEDISKEPSLSTQMLPLLKYSGQTSRLSASPTSQSTGDSLLAAVSP

Target ORF information:

RefSeq Version	XM_005640664.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640664.3

ORF Insert Sequence:

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4 ( XM_005640665.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640722.1 Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe31507
Clone ID Related Accession (Same CDS sequence)	XM_005640665.2 , XM_545645.5 , XM_005640664.2 , XM_005640664.3 , XM_005640665.3 , XM_545645.6
Accession Version	XM_005640665.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tubulin polyglutamylase TTLL4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640722.1
CDS	293..3895
Translation	MASARTEHYDIGLRRGNSLKQSGPSDTAPAPPPEKASEGRAWAQAHQQVKPIWKLERKHVGTLSAGLGPSVLGVPPQPAYFFCPGTLCSSGHSAVISGHGSSCYLRSLPDLFSSTLLYRRSSHRHKPYQQLESFCLRSSLPEKRPFSLPHKGLPVRLTANKAPSFTVSPMAQPMASSSTDRYLSLGAAGENPSGKCLAAAISGKIPSPLSSSYKPMLNNNSFMRPNSTKVPLSQATEGLKPVSSPKIHLVSWHHSGGTGDCALQPVEHKVPKSHGAVLDDAPAPGTLSAPSSLDTLTTSVASPQYNRSNLATRAEPHPCGLDGNFVSQALTREVRFTEAVRKLAARGFEKKPRQGCQLEQSRFMNPSLQCELLNRNRQWKPPTVGQQFPQEDAGANSRTLPSPSDALELDGTVFCTKRISIHLLASHASALSCSPACGSAIDSPPPGEDKTPEPPAPPQPLGVADVATRLSSIHLGQLGKEGPTEARGPDFPARDISSAADLQPDLGEAEDLEEELVDGLEDCCSHDENEEEEGDSECSSFSAVSTGESVAVISRSCAEILTKPLSSEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDAKLLRKAWESGSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNAEYQANADETACQGHKWALKALWNYLSQKGVNSDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPTAEDIVSSSGSSSSSSASLPSSPKDKCRMAPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHTGAICDSAPMWSLPASLLTIPKGDVGLSTFPKLESGKLGKHSFSEGSIPLCEDGTMPKPKKTQAGLCPVPGKPSSSKDSEDISKEPSLSTQMLPLLKYSGQTSRLSASPTSQSTGDSLLAAVSP

Target ORF information:

RefSeq Version	XM_005640665.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640665.3

ORF Insert Sequence:

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TTLL4 ( XM_545645.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_545645.2 Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X1, mRNA.

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CloneID	OCe31507
Clone ID Related Accession (Same CDS sequence)	XM_005640665.2 , XM_545645.5 , XM_005640664.2 , XM_005640664.3 , XM_005640665.3 , XM_545645.6
Accession Version	XM_545645.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	tubulin polyglutamylase TTLL4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_545645.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_545645.2
CDS	238..3840
Translation	MASARTEHYDIGLRRGNSLKQSGPSDTAPAPPPEKASEGRAWAQAHQQVKPIWKLERKHVGTLSAGLGPSVLGVPPQPAYFFCPGTLCSSGHSAVISGHGSSCYLRSLPDLFSSTLLYRRSSHRHKPYQQLESFCLRSSLPEKRPFSLPHKGLPVRLTANKAPSFTVSPMAQPMASSSTDRYLSLGAAGENPSGKCLAAAISGKIPSPLSSSYKPMLNNNSFMRPNSTKVPLSQATEGLKPVSSPKIHLVSWHHSGGTGDCALQPVEHKVPKSHGAVLDDAPAPGTLSAPSSLDTLTTSVASPQYNRSNLATRAEPHPCGLDGNFVSQALTREVRFTEAVRKLAARGFEKKPRQGCQLEQSRFMNPSLQCELLNRNRQWKPPTVGQQFPQEDAGANSRTLPSPSDALELDGTVFCTKRISIHLLASHASALSCSPACGSAIDSPPPGEDKTPEPPAPPQPLGVADVATRLSSIHLGQLGKEGPTEARGPDFPARDISSAADLQPDLGEAEDLEEELVDGLEDCCSHDENEEEEGDSECSSFSAVSTGESVAVISRSCAEILTKPLSSEKVVRPALIYSLFPNVPPTIYFGTRDERVEKLPWEQRKLLRWKMSTVTPNIVKQTIGRSHFKISKRNDDWLGCWGHHMKSPSFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKEFSFFPQSFILPQDAKLLRKAWESGSRQKWIVKPPASARGIGIQVIHKWSQLPKRRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLSNKFMHLTNYSVNKKNAEYQANADETACQGHKWALKALWNYLSQKGVNSDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNISPSLHSNSPLDISIKGQMIRDLLNLAGFVLPTAEDIVSSSGSSSSSSASLPSSPKDKCRMAPEHFTAQKMKKAYYLTQKIPDQDFYASVLDVLTPDDVRILVEMEDEFSRRGQFERIFPSRISSRYLRFFEQPRYFNILTTQWEQKYHGNKLKGVDLLRSWCYKGFHTGAICDSAPMWSLPASLLTIPKGDVGLSTFPKLESGKLGKHSFSEGSIPLCEDGTMPKPKKTQAGLCPVPGKPSSSKDSEDISKEPSLSTQMLPLLKYSGQTSRLSASPTSQSTGDSLLAAVSP

Target ORF information:

RefSeq Version	XM_545645.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris tubulin tyrosine ligase like 4 (TTLL4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_545645.6

ORF Insert Sequence:

ATGGCCTCAG CGAGAACAGA GCACTACGAT ATTGGCCTCC GCCGGGGAAA CAGCTTAAAG 
CAGAGCGGCC CCTCAGACAC GGCGCCTGCC CCACCACCGG AGAAAGCCTC TGAGGGCAGA 
GCCTGGGCTC AGGCCCATCA GCAGGTGAAG CCAATCTGGA AGCTGGAGAG AAAGCACGTG 
GGGACGCTTT CAGCAGGGTT GGGCCCCAGC GTCTTGGGTG TCCCACCCCA GCCAGCCTAT 
TTCTTTTGCC CGGGCACTCT GTGTAGCTCG GGGCACTCAG CTGTCATCTC AGGCCACGGC 
AGCTCCTGCT ACCTGCGTTC CCTCCCAGAC TTGTTCAGCA GCACGCTGCT GTACCGCCGC 
TCCAGCCACA GGCACAAACC CTACCAGCAG CTGGAGTCTT TCTGCTTGCG CTCAAGCCTG 
CCGGAAAAAA GACCTTTTTC TCTCCCTCAC AAGGGCCTCC CTGTCAGACT CACTGCCAAT 
AAGGCCCCTT CTTTCACGGT GTCCCCCATG GCCCAGCCCA TGGCGTCCTC GTCCACAGAT 
CGGTACCTCT CACTGGGAGC GGCTGGGGAG AACCCTTCGG GGAAGTGCCT GGCTGCTGCC 
ATCTCAGGGA AGATCCCATC TCCACTCTCC TCCTCCTACA AGCCCATGCT AAATAACAAC 
TCCTTCATGC GGCCAAATAG CACTAAAGTG CCTTTATCGC AGGCCACAGA GGGCCTGAAG 
CCAGTATCCT CACCCAAGAT CCATCTTGTC TCCTGGCATC ATTCGGGGGG CACCGGAGAC 
TGTGCACTCC AGCCTGTCGA ACACAAGGTA CCCAAGAGCC ATGGCGCTGT CCTAGATGAT 
GCCCCTGCCC CTGGCACCCT CTCTGCCCCT AGCTCCTTAG ACACTTTGAC CACCAGTGTT 
GCCTCTCCTC AGTACAACCG GAGTAACTTA GCCACTAGAG CAGAGCCACA TCCTTGTGGC 
CTGGATGGCA ACTTTGTTTC TCAGGCTCTG ACCAGGGAGG TTCGGTTCAC TGAGGCGGTG 
AGGAAATTGG CCGCAAGAGG CTTTGAGAAG AAGCCAAGGC AAGGCTGCCA GTTGGAACAA 
TCTCGTTTCA TGAACCCCAG CTTGCAGTGT GAGCTCCTCA ACAGGAACCG GCAGTGGAAA 
CCTCCCACCG TAGGCCAGCA GTTCCCCCAG GAGGATGCTG GAGCTAACAG TAGGACCCTG 
CCCAGCCCCT CGGACGCCCT GGAGCTGGAT GGTACAGTCT TCTGTACCAA ACGCATCAGC 
ATTCACCTCC TTGCCTCACA TGCCAGCGCG CTCAGCTGCA GCCCTGCCTG TGGATCTGCC 
ATTGACTCCC CACCACCCGG AGAAGACAAA ACTCCTGAGC CACCTGCTCC TCCTCAGCCC 
CTGGGTGTAG CTGATGTGGC CACCCGCCTC TCTTCCATCC ACTTGGGCCA ACTTGGGAAG 
GAGGGGCCTA CGGAAGCCAG AGGACCGGAC TTCCCTGCCA GGGATATCAG TTCAGCTGCA 
GACCTCCAGC CAGACCTGGG TGAGGCTGAA GATCTAGAAG AAGAGCTAGT AGATGGTTTG 
GAGGACTGCT GTAGCCATGA TGAAAATGAA GAGGAGGAAG GAGACTCTGA GTGCTCTTCG 
TTCAGTGCTG TCTCCACTGG TGAGTCAGTA GCAGTGATCT CTCGGAGCTG TGCAGAGATT 
CTGACCAAAC CCCTCTCCAG TGAGAAAGTG GTCCGACCAG CGCTCATCTA CAGCCTCTTT 
CCCAACGTGC CCCCTACCAT CTATTTTGGC ACCCGGGATG AGAGAGTGGA GAAACTTCCC 
TGGGAGCAGA GGAAGCTGCT CCGGTGGAAG ATGAGCACAG TGACTCCCAA CATTGTCAAG 
CAGACCATTG GACGGTCCCA CTTCAAAATC AGCAAGAGAA ATGACGACTG GCTAGGCTGC 
TGGGGTCACC ACATGAAGTC TCCTAGTTTC CGATCCATTC GGGAGCATCA GAAGCTAAAC 
CACTTCCCAG GTTCCTTCCA GATTGGACGA AAGGACCGAC TGTGGCGGAA CCTGTCTCGC 
ATGCAGAGCC GCTTTGGCAA GAAGGAGTTC AGCTTCTTCC CCCAGTCCTT CATCCTGCCC 
CAGGATGCCA AGCTCCTGCG CAAAGCCTGG GAGAGCGGCA GCCGCCAGAA GTGGATTGTT 
AAGCCTCCAG CGTCAGCCCG GGGCATTGGC ATCCAGGTCA TTCACAAGTG GAGTCAGCTC 
CCCAAGCGAA GGCCCCTCCT GGTACAGAGG TATCTACACA AACCCTACCT CATCAGTGGC 
AGCAAGTTCG ATCTGCGAAT CTATGTTTAC GTCACCTCCT ACGATCCCCT CCGGATTTAC 
CTCTTTTCAG ATGGACTTGT CCGCTTTGCC AGTTGCAAGT ATTCCCCTTC CATGAAGAGC 
CTCAGCAACA AGTTCATGCA CCTGACCAAC TACAGTGTCA ATAAAAAGAA TGCCGAGTAC 
CAGGCCAATG CAGATGAAAC GGCCTGCCAG GGCCACAAAT GGGCGCTGAA GGCTTTGTGG 
AACTACCTGA GCCAGAAGGG AGTCAATAGT GATGCCATCT GGGAGAAGAT AAAGGACGTT 
GTTGTCAAAA CCATCATCTC GTCAGAGCCC TATGTGACCA GCCTACTGAA GATGTACGTG 
CGGCGGCCAT ACAGCTGCCA TGAGCTCTTT GGCTTTGACA TCATGCTGGA CGAGAACCTC 
AAGCCCTGGG TCCTGGAAGT CAACATTTCC CCGAGCCTCC ACTCCAACTC TCCGCTGGAC 
ATCAGCATCA AAGGCCAGAT GATCCGTGAC CTTCTGAACC TGGCAGGCTT TGTTCTGCCC 
ACCGCAGAGG ATATCGTTTC CAGCTCTGGC AGCTCCAGCA GCTCCAGTGC CAGCTTGCCC 
AGCTCCCCCA AGGATAAATG TCGAATGGCT CCAGAGCATT TCACCGCACA GAAGATGAAG 
AAGGCCTATT ATCTGACCCA GAAAATTCCC GATCAGGACT TCTATGCATC TGTGCTGGAT 
GTCCTGACGC CAGATGATGT TCGGATTCTG GTTGAGATGG AGGATGAGTT TTCTCGCCGT 
GGTCAATTTG AACGAATTTT TCCTTCTCGA ATCTCTTCTC GCTATCTCCG CTTTTTTGAG 
CAGCCACGAT ACTTCAACAT TCTCACCACC CAATGGGAAC AGAAGTACCA TGGCAACAAG 
CTCAAAGGAG TAGATCTGCT TCGGAGTTGG TGCTACAAAG GGTTCCACAC AGGAGCCATC 
TGTGATTCTG CTCCAATGTG GTCTCTCCCA GCATCACTTC TGACCATCCC AAAGGGTGAC 
GTGGGACTCA GCACTTTCCC CAAACTAGAG AGTGGCAAGC TGGGAAAACA CAGCTTCTCC 
GAGGGAAGCA TACCACTCTG TGAAGATGGG ACCATGCCTA AGCCCAAGAA GACCCAAGCT 
GGCCTTTGTC CTGTTCCCGG GAAACCCAGT TCTTCCAAGG ACAGTGAGGA CATCAGCAAA 
GAGCCCAGCC TCTCTACCCA GATGTTACCT CTGTTGAAGT ACTCTGGGCA GACTTCGAGA 
CTCTCTGCTT CCCCCACCTC CCAGTCAACC GGTGACTCAC TCCTGGCTGC TGTGAGCCCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TTLL4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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