PFKL cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PFKL
Entrez Gene ID	487797
Full Name	phosphofructokinase, liver type
General protein information	Preferred Names phosphofructokinase, liver type Names ATP-dependent 6-phosphofructokinase, liver type
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_544922.7	XP_544922.3	ATP-dependent 6-phosphofructokinase, liver type isoform X1
XM_022413211.1	XP_022268919.1	ATP-dependent 6-phosphofructokinase, liver type isoform X2
XM_022413210.1	XP_022268918.1	ATP-dependent 6-phosphofructokinase, liver type isoform X2
XM_544922.6	XP_544922.3	ATP-dependent 6-phosphofructokinase, liver type

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PFKL	XP_001150758.1
Bos taurus (cattle)	PFKL	NP_001073713.1
Rattus norvegicus (Norway rat)	Pfkl	NP_037322.1
Danio rerio (zebrafish)	LOC570106	XP_005172606.1
Saccharomyces cerevisiae (baker's yeast)	PFK2	NP_013932.1
Eremothecium gossypii	AGOS_AFL185W	NP_985365.2
Neurospora crassa	NCU00629	XP_965769.2
Homo sapiens (human)	PFKL	NP_002617.3
Magnaporthe oryzae (rice blast fungus)	MGG_02653	XP_003721131.1
Canis lupus familiaris (dog)	PFKL	XP_544922.3
Mus musculus (house mouse)	Pfkl	NP_032852.2
Gallus gallus (chicken)	PFKL	XP_004937046.1
	LOC722173	XP_002803157.1
Kluyveromyces lactis	KLLA0F06248g	XP_455363.1
Schizosaccharomyces pombe (fission yeast)	pfk1	NP_595946.1

Gene Ontology

The following PFKL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe29499	XM_544922.7 Latest version!	Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe283930	XM_022413211.1 Latest version!	Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe283930	XM_022413210.1 Latest version!	Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OCe29499	XM_544922.6	Canis lupus familiaris phosphofructokinase, liver (PFKL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PFKL ( XM_544922.7 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_544922.3 Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe29499
Clone ID Related Accession (Same CDS sequence)	XM_544922.7 , XM_544922.6
Accession Version	XM_544922.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ATP-dependent 6-phosphofructokinase, liver type isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_544922.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGG TGGACCTGGA GAAGCTGCGG ATGTCCGGGG CCGGCAAGGC CATCGGCGTG 
CTGACCAGCG GCGGCGACGC GCAAGGAATG AACGCGGCCG TCAGAGCTGT GACGCGCATG 
GGCATTTACG TGGGAGCCAA GGTCTTCCTC ATCTACGAGG GCTATGAGGG TCTCGTGGAA 
GGAGGTGAGA ACATCAAGCA GGCCAACTGG CTCAGCGTCT CCAACATCAT CCAGCTGGGC 
GGCACCGTCA TCGGCAGCGC GCGCTGTAAG GCCTTCACCA CCCGGGAGGG ACGCCTGGCG 
GCGGCCTACA ACCTGGTGCA GCGCGGCATC ACCAACCTGT GCGTCATCGG CGGGGACGGC 
AGCCTGACGG GCGCCGACAT CTTCCGCAGC GAGTGGGGCA GCCTGCTGGA GGAGCTGGTG 
GCGGCAGGCA GGATCTCGGA GAGCACGGCG CAGACCTACT CGCACCTGAG CATCGTGGGG 
CTGGTGGGCT CCATCGACAA CGACTTCTGC GGCACCGACA TGACCATCGG CACGGACTCG 
GCCCTGCACC GCATCATGGA GGTCATCGAC GCCATCACCA CCACCGCCCA GAGCCACCAG 
AGGACCTTCG TGCTGGAGGT GATGGGACGG CACTGCGGGT ACCTGGCCCT GGTGTCCGCC 
CTGGCCTCGG GGGCCGACTG GCTCTTCATC CCCGAGGCGC CCCCCGAGGA CGGCTGGGAG 
AACTTCATGT GCGAGAGGCT CGGAGAGACT CGGAGCAGGG GGTCCCGACT GAACATCATC 
ATCATCGCGG AGGGCGCCAT CGACCGCAAC GGGAAGCCCA TCTCGTCCCG CTACGTGAAG 
GACCTGGTGG TCCAGAGGCT GGGCTTCGAC ACGCGTGTGA CCGTGCTGGG CCACGTGCAG 
AGGGGAGGGA CCCCTTCGGC ATTTGACCGC ATCCTGAGCA GCAAGATGGG CATGGAGGCC 
GTCATGGCGC TGCTGGAGGC CACAGCGGAC ACGCAGGCCT GCGTGGTCAG CCTCTCGGGG 
AACCAGTCCG TGCGGCTGCC CCTCATGGAG TGTGTGCAGG TGACCAAGGA GGTGCAGAAG 
GCCATGGACG AGAAGCGGTT CGACGAGGCC ATCCAGCTCC GCGGCAGGAG CTTTGAGAAC 
AACTGGAACA TTTACAAGCT GCTTGCACAC CAGAAGGTCT CCAAGGAGAA GACCAACTTC 
TCCTTGGCCA TCCTGAACGT GGGGGCCCCG GCGGCTGGCA TGAACGCGGC CGTGCGCTCG 
GCGGTGCGGT CGGGCATCTC CCAGGGCCAC ACGGTGTACG TCGTACACGA CGGCTTCGAG 
GGTCTGGCCA AGGGTCAGGT ACAAGAGGTG AGTTGGCACG ACGTGGCCGG CTGGCTGGGC 
CGTGGCGGCT CCATGCTGGG GACGAAGAGG ACGCTGCCCA GGAGCTACAT GGAGAAGATA 
GTGGACAACA TTCGCACCTA CAACATCCAC GCCCTGCTGG TCATCGGGGG CTTTGAGGCC 
TACGAGGGGG TGCTGCAGCT GGTGGAGGCG CGCGGCCGCT ACGAGGAGCT CTGCATCGTC 
ATGTGCGTCA TCCCGGCCAC CATCAGCAAC AACGTGCCGG GCACCGACTT CAGTCTGGGC 
TCAGACACCG CCGTCAACGC CGCCATGGAG AGCTGTGACC GCATCAAGCA GTCGGCGTCG 
GGCACCAAGC GCCGCGTGTT CATCGTGGAG ACCATGGGCG GCTACTGTGG CTACCTGGCC 
ACGGTGACTG GCATTGCTGT GGGGGCCGAC GCCGCCTACG TCTTCGAGGA CCCTTTCAAC 
ATCCAAGACC TGAAAGCCAA CGTGGAGCAC ATGACCGAGA AGATGAAGAC AGACATCCAG 
AGGGGCCTCG TGCTCCGAAA CGAGAAGTGC CACGAGCACT ACACCACGGA GTTTCTGTAT 
AACCTGTACT CCTCCGAGGG GAAGGGCGTC TTTGACTGTA GGACCAACGT TCTGGGCCAC 
CTGCAGCAGG GTGGGGCTCC GACCCCCTTT GACCGGAACT ACGGCACGAA GCTGGGGGTG 
AAGGCGATGC TCTGGATGTC AGAGAAGCTG CGGGCCGTCT ACCGCAACGG CCGCGTGTTC 
GCCAACGCCC CCGATTCGGC CTGCGTGATC GGCCTGCAGA AGAAGGCGGT GGCCTTCAGC 
CCCGTCACCG AGCTCAAGGA GGTCACGGAC TTTGAGCACC GCATGCCCCG GGAGCAGTGG 
TGGCTGAACC TGCGGCTCAT GCTGAAGATG CTGGCGCACT ACCGCATCAG CATGGCCGAC 
TACGTGTCCG GGGAGCTGGA GCACGTCACC CGCCGCAGCC TCAGCATCGA CAAGGGCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544922.3
CDS	1..2343
Translation	MATVDLEKLRMSGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTVIGSARCKAFTTREGRLAAAYNLVQRGITNLCVIGGDGSLTGADIFRSEWGSLLEELVAAGRISESTAQTYSHLSIVGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSRYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATADTQACVVSLSGNQSVRLPLMECVQVTKEVQKAMDEKRFDEAIQLRGRSFENNWNIYKLLAHQKVSKEKTNFSLAILNVGAPAAGMNAAVRSAVRSGISQGHTVYVVHDGFEGLAKGQVQEVSWHDVAGWLGRGGSMLGTKRTLPRSYMEKIVDNIRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIQDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWMSEKLRAVYRNGRVFANAPDSACVIGLQKKAVAFSPVTELKEVTDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVTRRSLSIDKGF

Target ORF information:

RefSeq Version	XM_544922.7
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544922.7

ORF Insert Sequence:

ATGGCCACGG TGGACCTGGA GAAGCTGCGG ATGTCCGGGG CCGGCAAGGC CATCGGCGTG 
CTGACCAGCG GCGGCGACGC GCAAGGAATG AACGCGGCCG TCAGAGCTGT GACGCGCATG 
GGCATTTACG TGGGAGCCAA GGTCTTCCTC ATCTACGAGG GCTATGAGGG TCTCGTGGAA 
GGAGGTGAGA ACATCAAGCA GGCCAACTGG CTCAGCGTCT CCAACATCAT CCAGCTGGGC 
GGCACCGTCA TCGGCAGCGC GCGCTGTAAG GCCTTCACCA CCCGGGAGGG ACGCCTGGCG 
GCGGCCTACA ACCTGGTGCA GCGCGGCATC ACCAACCTGT GCGTCATCGG CGGGGACGGC 
AGCCTGACGG GCGCCGACAT CTTCCGCAGC GAGTGGGGCA GCCTGCTGGA GGAGCTGGTG 
GCGGCAGGCA GGATCTCGGA GAGCACGGCG CAGACCTACT CGCACCTGAG CATCGTGGGG 
CTGGTGGGCT CCATCGACAA CGACTTCTGC GGCACCGACA TGACCATCGG CACGGACTCG 
GCCCTGCACC GCATCATGGA GGTCATCGAC GCCATCACCA CCACCGCCCA GAGCCACCAG 
AGGACCTTCG TGCTGGAGGT GATGGGACGG CACTGCGGGT ACCTGGCCCT GGTGTCCGCC 
CTGGCCTCGG GGGCCGACTG GCTCTTCATC CCCGAGGCGC CCCCCGAGGA CGGCTGGGAG 
AACTTCATGT GCGAGAGGCT CGGAGAGACT CGGAGCAGGG GGTCCCGACT GAACATCATC 
ATCATCGCGG AGGGCGCCAT CGACCGCAAC GGGAAGCCCA TCTCGTCCCG CTACGTGAAG 
GACCTGGTGG TCCAGAGGCT GGGCTTCGAC ACGCGTGTGA CCGTGCTGGG CCACGTGCAG 
AGGGGAGGGA CCCCTTCGGC ATTTGACCGC ATCCTGAGCA GCAAGATGGG CATGGAGGCC 
GTCATGGCGC TGCTGGAGGC CACAGCGGAC ACGCAGGCCT GCGTGGTCAG CCTCTCGGGG 
AACCAGTCCG TGCGGCTGCC CCTCATGGAG TGTGTGCAGG TGACCAAGGA GGTGCAGAAG 
GCCATGGACG AGAAGCGGTT CGACGAGGCC ATCCAGCTCC GCGGCAGGAG CTTTGAGAAC 
AACTGGAACA TTTACAAGCT GCTTGCACAC CAGAAGGTCT CCAAGGAGAA GACCAACTTC 
TCCTTGGCCA TCCTGAACGT GGGGGCCCCG GCGGCTGGCA TGAACGCGGC CGTGCGCTCG 
GCGGTGCGGT CGGGCATCTC CCAGGGCCAC ACGGTGTACG TCGTACACGA CGGCTTCGAG 
GGTCTGGCCA AGGGTCAGGT ACAAGAGGTG AGTTGGCACG ACGTGGCCGG CTGGCTGGGC 
CGTGGCGGCT CCATGCTGGG GACGAAGAGG ACGCTGCCCA GGAGCTACAT GGAGAAGATA 
GTGGACAACA TTCGCACCTA CAACATCCAC GCCCTGCTGG TCATCGGGGG CTTTGAGGCC 
TACGAGGGGG TGCTGCAGCT GGTGGAGGCG CGCGGCCGCT ACGAGGAGCT CTGCATCGTC 
ATGTGCGTCA TCCCGGCCAC CATCAGCAAC AACGTGCCGG GCACCGACTT CAGTCTGGGC 
TCAGACACCG CCGTCAACGC CGCCATGGAG AGCTGTGACC GCATCAAGCA GTCGGCGTCG 
GGCACCAAGC GCCGCGTGTT CATCGTGGAG ACCATGGGCG GCTACTGTGG CTACCTGGCC 
ACGGTGACTG GCATTGCTGT GGGGGCCGAC GCCGCCTACG TCTTCGAGGA CCCTTTCAAC 
ATCCAAGACC TGAAAGCCAA CGTGGAGCAC ATGACCGAGA AGATGAAGAC AGACATCCAG 
AGGGGCCTCG TGCTCCGAAA CGAGAAGTGC CACGAGCACT ACACCACGGA GTTTCTGTAT 
AACCTGTACT CCTCCGAGGG GAAGGGCGTC TTTGACTGTA GGACCAACGT TCTGGGCCAC 
CTGCAGCAGG GTGGGGCTCC GACCCCCTTT GACCGGAACT ACGGCACGAA GCTGGGGGTG 
AAGGCGATGC TCTGGATGTC AGAGAAGCTG CGGGCCGTCT ACCGCAACGG CCGCGTGTTC 
GCCAACGCCC CCGATTCGGC CTGCGTGATC GGCCTGCAGA AGAAGGCGGT GGCCTTCAGC 
CCCGTCACCG AGCTCAAGGA GGTCACGGAC TTTGAGCACC GCATGCCCCG GGAGCAGTGG 
TGGCTGAACC TGCGGCTCAT GCTGAAGATG CTGGCGCACT ACCGCATCAG CATGGCCGAC 
TACGTGTCCG GGGAGCTGGA GCACGTCACC CGCCGCAGCC TCAGCATCGA CAAGGGCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKL ( XM_544922.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_544922.3 Canis lupus familiaris phosphofructokinase, liver (PFKL), mRNA.

Price & Availability↑

CloneID	OCe29499
Clone ID Related Accession (Same CDS sequence)	XM_544922.7 , XM_544922.6
Accession Version	XM_544922.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ATP-dependent 6-phosphofructokinase, liver type
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726114.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_544922.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGG TGGACCTGGA GAAGCTGCGG ATGTCCGGGG CCGGCAAGGC CATCGGCGTG 
CTGACCAGCG GCGGCGACGC GCAAGGAATG AACGCGGCCG TCAGAGCTGT GACGCGCATG 
GGCATTTACG TGGGAGCCAA GGTCTTCCTC ATCTACGAGG GCTATGAGGG TCTCGTGGAA 
GGAGGTGAGA ACATCAAGCA GGCCAACTGG CTCAGCGTCT CCAACATCAT CCAGCTGGGC 
GGCACCGTCA TCGGCAGCGC GCGCTGTAAG GCCTTCACCA CCCGGGAGGG ACGCCTGGCG 
GCGGCCTACA ACCTGGTGCA GCGCGGCATC ACCAACCTGT GCGTCATCGG CGGGGACGGC 
AGCCTGACGG GCGCCGACAT CTTCCGCAGC GAGTGGGGCA GCCTGCTGGA GGAGCTGGTG 
GCGGCAGGCA GGATCTCGGA GAGCACGGCG CAGACCTACT CGCACCTGAG CATCGTGGGG 
CTGGTGGGCT CCATCGACAA CGACTTCTGC GGCACCGACA TGACCATCGG CACGGACTCG 
GCCCTGCACC GCATCATGGA GGTCATCGAC GCCATCACCA CCACCGCCCA GAGCCACCAG 
AGGACCTTCG TGCTGGAGGT GATGGGACGG CACTGCGGGT ACCTGGCCCT GGTGTCCGCC 
CTGGCCTCGG GGGCCGACTG GCTCTTCATC CCCGAGGCGC CCCCCGAGGA CGGCTGGGAG 
AACTTCATGT GCGAGAGGCT CGGAGAGACT CGGAGCAGGG GGTCCCGACT GAACATCATC 
ATCATCGCGG AGGGCGCCAT CGACCGCAAC GGGAAGCCCA TCTCGTCCCG CTACGTGAAG 
GACCTGGTGG TCCAGAGGCT GGGCTTCGAC ACGCGTGTGA CCGTGCTGGG CCACGTGCAG 
AGGGGAGGGA CCCCTTCGGC ATTTGACCGC ATCCTGAGCA GCAAGATGGG CATGGAGGCC 
GTCATGGCGC TGCTGGAGGC CACAGCGGAC ACGCAGGCCT GCGTGGTCAG CCTCTCGGGG 
AACCAGTCCG TGCGGCTGCC CCTCATGGAG TGTGTGCAGG TGACCAAGGA GGTGCAGAAG 
GCCATGGACG AGAAGCGGTT CGACGAGGCC ATCCAGCTCC GCGGCAGGAG CTTTGAGAAC 
AACTGGAACA TTTACAAGCT GCTTGCACAC CAGAAGGTCT CCAAGGAGAA GACCAACTTC 
TCCTTGGCCA TCCTGAACGT GGGGGCCCCG GCGGCTGGCA TGAACGCGGC CGTGCGCTCG 
GCGGTGCGGT CGGGCATCTC CCAGGGCCAC ACGGTGTACG TCGTACACGA CGGCTTCGAG 
GGTCTGGCCA AGGGTCAGGT ACAAGAGGTG AGTTGGCACG ACGTGGCCGG CTGGCTGGGC 
CGTGGCGGCT CCATGCTGGG GACGAAGAGG ACGCTGCCCA GGAGCTACAT GGAGAAGATA 
GTGGACAACA TTCGCACCTA CAACATCCAC GCCCTGCTGG TCATCGGGGG CTTTGAGGCC 
TACGAGGGGG TGCTGCAGCT GGTGGAGGCG CGCGGCCGCT ACGAGGAGCT CTGCATCGTC 
ATGTGCGTCA TCCCGGCCAC CATCAGCAAC AACGTGCCGG GCACCGACTT CAGTCTGGGC 
TCAGACACCG CCGTCAACGC CGCCATGGAG AGCTGTGACC GCATCAAGCA GTCGGCGTCG 
GGCACCAAGC GCCGCGTGTT CATCGTGGAG ACCATGGGCG GCTACTGTGG CTACCTGGCC 
ACGGTGACTG GCATTGCTGT GGGGGCCGAC GCCGCCTACG TCTTCGAGGA CCCTTTCAAC 
ATCCAAGACC TGAAAGCCAA CGTGGAGCAC ATGACCGAGA AGATGAAGAC AGACATCCAG 
AGGGGCCTCG TGCTCCGAAA CGAGAAGTGC CACGAGCACT ACACCACGGA GTTTCTGTAT 
AACCTGTACT CCTCCGAGGG GAAGGGCGTC TTTGACTGTA GGACCAACGT TCTGGGCCAC 
CTGCAGCAGG GTGGGGCTCC GACCCCCTTT GACCGGAACT ACGGCACGAA GCTGGGGGTG 
AAGGCGATGC TCTGGATGTC AGAGAAGCTG CGGGCCGTCT ACCGCAACGG CCGCGTGTTC 
GCCAACGCCC CCGATTCGGC CTGCGTGATC GGCCTGCAGA AGAAGGCGGT GGCCTTCAGC 
CCCGTCACCG AGCTCAAGGA GGTCACGGAC TTTGAGCACC GCATGCCCCG GGAGCAGTGG 
TGGCTGAACC TGCGGCTCAT GCTGAAGATG CTGGCGCACT ACCGCATCAG CATGGCCGAC 
TACGTGTCCG GGGAGCTGGA GCACGTCACC CGCCGCAGCC TCAGCATCGA CAAGGGCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544922.3
CDS	95..2437
Translation	MATVDLEKLRMSGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTVIGSARCKAFTTREGRLAAAYNLVQRGITNLCVIGGDGSLTGADIFRSEWGSLLEELVAAGRISESTAQTYSHLSIVGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSRYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATADTQACVVSLSGNQSVRLPLMECVQVTKEVQKAMDEKRFDEAIQLRGRSFENNWNIYKLLAHQKVSKEKTNFSLAILNVGAPAAGMNAAVRSAVRSGISQGHTVYVVHDGFEGLAKGQVQEVSWHDVAGWLGRGGSMLGTKRTLPRSYMEKIVDNIRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIQDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWMSEKLRAVYRNGRVFANAPDSACVIGLQKKAVAFSPVTELKEVTDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVTRRSLSIDKGF

Target ORF information:

RefSeq Version	XM_544922.6
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phosphofructokinase, liver (PFKL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544922.6

ORF Insert Sequence:

ATGGCCACGG TGGACCTGGA GAAGCTGCGG ATGTCCGGGG CCGGCAAGGC CATCGGCGTG 
CTGACCAGCG GCGGCGACGC GCAAGGAATG AACGCGGCCG TCAGAGCTGT GACGCGCATG 
GGCATTTACG TGGGAGCCAA GGTCTTCCTC ATCTACGAGG GCTATGAGGG TCTCGTGGAA 
GGAGGTGAGA ACATCAAGCA GGCCAACTGG CTCAGCGTCT CCAACATCAT CCAGCTGGGC 
GGCACCGTCA TCGGCAGCGC GCGCTGTAAG GCCTTCACCA CCCGGGAGGG ACGCCTGGCG 
GCGGCCTACA ACCTGGTGCA GCGCGGCATC ACCAACCTGT GCGTCATCGG CGGGGACGGC 
AGCCTGACGG GCGCCGACAT CTTCCGCAGC GAGTGGGGCA GCCTGCTGGA GGAGCTGGTG 
GCGGCAGGCA GGATCTCGGA GAGCACGGCG CAGACCTACT CGCACCTGAG CATCGTGGGG 
CTGGTGGGCT CCATCGACAA CGACTTCTGC GGCACCGACA TGACCATCGG CACGGACTCG 
GCCCTGCACC GCATCATGGA GGTCATCGAC GCCATCACCA CCACCGCCCA GAGCCACCAG 
AGGACCTTCG TGCTGGAGGT GATGGGACGG CACTGCGGGT ACCTGGCCCT GGTGTCCGCC 
CTGGCCTCGG GGGCCGACTG GCTCTTCATC CCCGAGGCGC CCCCCGAGGA CGGCTGGGAG 
AACTTCATGT GCGAGAGGCT CGGAGAGACT CGGAGCAGGG GGTCCCGACT GAACATCATC 
ATCATCGCGG AGGGCGCCAT CGACCGCAAC GGGAAGCCCA TCTCGTCCCG CTACGTGAAG 
GACCTGGTGG TCCAGAGGCT GGGCTTCGAC ACGCGTGTGA CCGTGCTGGG CCACGTGCAG 
AGGGGAGGGA CCCCTTCGGC ATTTGACCGC ATCCTGAGCA GCAAGATGGG CATGGAGGCC 
GTCATGGCGC TGCTGGAGGC CACAGCGGAC ACGCAGGCCT GCGTGGTCAG CCTCTCGGGG 
AACCAGTCCG TGCGGCTGCC CCTCATGGAG TGTGTGCAGG TGACCAAGGA GGTGCAGAAG 
GCCATGGACG AGAAGCGGTT CGACGAGGCC ATCCAGCTCC GCGGCAGGAG CTTTGAGAAC 
AACTGGAACA TTTACAAGCT GCTTGCACAC CAGAAGGTCT CCAAGGAGAA GACCAACTTC 
TCCTTGGCCA TCCTGAACGT GGGGGCCCCG GCGGCTGGCA TGAACGCGGC CGTGCGCTCG 
GCGGTGCGGT CGGGCATCTC CCAGGGCCAC ACGGTGTACG TCGTACACGA CGGCTTCGAG 
GGTCTGGCCA AGGGTCAGGT ACAAGAGGTG AGTTGGCACG ACGTGGCCGG CTGGCTGGGC 
CGTGGCGGCT CCATGCTGGG GACGAAGAGG ACGCTGCCCA GGAGCTACAT GGAGAAGATA 
GTGGACAACA TTCGCACCTA CAACATCCAC GCCCTGCTGG TCATCGGGGG CTTTGAGGCC 
TACGAGGGGG TGCTGCAGCT GGTGGAGGCG CGCGGCCGCT ACGAGGAGCT CTGCATCGTC 
ATGTGCGTCA TCCCGGCCAC CATCAGCAAC AACGTGCCGG GCACCGACTT CAGTCTGGGC 
TCAGACACCG CCGTCAACGC CGCCATGGAG AGCTGTGACC GCATCAAGCA GTCGGCGTCG 
GGCACCAAGC GCCGCGTGTT CATCGTGGAG ACCATGGGCG GCTACTGTGG CTACCTGGCC 
ACGGTGACTG GCATTGCTGT GGGGGCCGAC GCCGCCTACG TCTTCGAGGA CCCTTTCAAC 
ATCCAAGACC TGAAAGCCAA CGTGGAGCAC ATGACCGAGA AGATGAAGAC AGACATCCAG 
AGGGGCCTCG TGCTCCGAAA CGAGAAGTGC CACGAGCACT ACACCACGGA GTTTCTGTAT 
AACCTGTACT CCTCCGAGGG GAAGGGCGTC TTTGACTGTA GGACCAACGT TCTGGGCCAC 
CTGCAGCAGG GTGGGGCTCC GACCCCCTTT GACCGGAACT ACGGCACGAA GCTGGGGGTG 
AAGGCGATGC TCTGGATGTC AGAGAAGCTG CGGGCCGTCT ACCGCAACGG CCGCGTGTTC 
GCCAACGCCC CCGATTCGGC CTGCGTGATC GGCCTGCAGA AGAAGGCGGT GGCCTTCAGC 
CCCGTCACCG AGCTCAAGGA GGTCACGGAC TTTGAGCACC GCATGCCCCG GGAGCAGTGG 
TGGCTGAACC TGCGGCTCAT GCTGAAGATG CTGGCGCACT ACCGCATCAG CATGGCCGAC 
TACGTGTCCG GGGAGCTGGA GCACGTCACC CGCCGCAGCC TCAGCATCGA CAAGGGCTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKL ( XM_022413211.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022268919.1 Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe283930
Clone ID Related Accession (Same CDS sequence)	XM_022413211.1 , XM_022413210.1
Accession Version	XM_022413211.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ATP-dependent 6-phosphofructokinase, liver type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCGG CCGTCAGAGC TGTGACGCGC ATGGGCATTT ACGTGGGAGC CAAGGTCTTC 
CTCATCTACG AGGGCTATGA GGGTCTCGTG GAAGGAGGTG AGAACATCAA GCAGGCCAAC 
TGGCTCAGCG TCTCCAACAT CATCCAGCTG GGCGGCACCG TCATCGGCAG CGCGCGCTGT 
AAGGCCTTCA CCACCCGGGA GGGACGCCTG GCGGCGGCCT ACAACCTGGT GCAGCGCGGC 
ATCACCAACC TGTGCGTCAT CGGCGGGGAC GGCAGCCTGA CGGGCGCCGA CATCTTCCGC 
AGCGAGTGGG GCAGCCTGCT GGAGGAGCTG GTGGCGGCAG GCAGGATCTC GGAGAGCACG 
GCGCAGACCT ACTCGCACCT GAGCATCGTG GGGCTGGTGG GCTCCATCGA CAACGACTTC 
TGCGGCACCG ACATGACCAT CGGCACGGAC TCGGCCCTGC ACCGCATCAT GGAGGTCATC 
GACGCCATCA CCACCACCGC CCAGAGCCAC CAGAGGACCT TCGTGCTGGA GGTGATGGGA 
CGGCACTGCG GGTACCTGGC CCTGGTGTCC GCCCTGGCCT CGGGGGCCGA CTGGCTCTTC 
ATCCCCGAGG CGCCCCCCGA GGACGGCTGG GAGAACTTCA TGTGCGAGAG GCTCGGAGAG 
ACTCGGAGCA GGGGGTCCCG ACTGAACATC ATCATCATCG CGGAGGGCGC CATCGACCGC 
AACGGGAAGC CCATCTCGTC CCGCTACGTG AAGGACCTGG TGGTCCAGAG GCTGGGCTTC 
GACACGCGTG TGACCGTGCT GGGCCACGTG CAGAGGGGAG GGACCCCTTC GGCATTTGAC 
CGCATCCTGA GCAGCAAGAT GGGCATGGAG GCCGTCATGG CGCTGCTGGA GGCCACAGCG 
GACACGCAGG CCTGCGTGGT CAGCCTCTCG GGGAACCAGT CCGTGCGGCT GCCCCTCATG 
GAGTGTGTGC AGGTGACCAA GGAGGTGCAG AAGGCCATGG ACGAGAAGCG GTTCGACGAG 
GCCATCCAGC TCCGCGGCAG GAGCTTTGAG AACAACTGGA ACATTTACAA GCTGCTTGCA 
CACCAGAAGG TCTCCAAGGA GAAGACCAAC TTCTCCTTGG CCATCCTGAA CGTGGGGGCC 
CCGGCGGCTG GCATGAACGC GGCCGTGCGC TCGGCGGTGC GGTCGGGCAT CTCCCAGGGC 
CACACGGTGT ACGTCGTACA CGACGGCTTC GAGGGTCTGG CCAAGGGTCA GGTACAAGAG 
GTGAGTTGGC ACGACGTGGC CGGCTGGCTG GGCCGTGGCG GCTCCATGCT GGGGACGAAG 
AGGACGCTGC CCAGGAGCTA CATGGAGAAG ATAGTGGACA ACATTCGCAC CTACAACATC 
CACGCCCTGC TGGTCATCGG GGGCTTTGAG GCCTACGAGG GGGTGCTGCA GCTGGTGGAG 
GCGCGCGGCC GCTACGAGGA GCTCTGCATC GTCATGTGCG TCATCCCGGC CACCATCAGC 
AACAACGTGC CGGGCACCGA CTTCAGTCTG GGCTCAGACA CCGCCGTCAA CGCCGCCATG 
GAGAGCTGTG ACCGCATCAA GCAGTCGGCG TCGGGCACCA AGCGCCGCGT GTTCATCGTG 
GAGACCATGG GCGGCTACTG TGGCTACCTG GCCACGGTGA CTGGCATTGC TGTGGGGGCC 
GACGCCGCCT ACGTCTTCGA GGACCCTTTC AACATCCAAG ACCTGAAAGC CAACGTGGAG 
CACATGACCG AGAAGATGAA GACAGACATC CAGAGGGGCC TCGTGCTCCG AAACGAGAAG 
TGCCACGAGC ACTACACCAC GGAGTTTCTG TATAACCTGT ACTCCTCCGA GGGGAAGGGC 
GTCTTTGACT GTAGGACCAA CGTTCTGGGC CACCTGCAGC AGGGTGGGGC TCCGACCCCC 
TTTGACCGGA ACTACGGCAC GAAGCTGGGG GTGAAGGCGA TGCTCTGGAT GTCAGAGAAG 
CTGCGGGCCG TCTACCGCAA CGGCCGCGTG TTCGCCAACG CCCCCGATTC GGCCTGCGTG 
ATCGGCCTGC AGAAGAAGGC GGTGGCCTTC AGCCCCGTCA CCGAGCTCAA GGAGGTCACG 
GACTTTGAGC ACCGCATGCC CCGGGAGCAG TGGTGGCTGA ACCTGCGGCT CATGCTGAAG 
ATGCTGGCGC ACTACCGCAT CAGCATGGCC GACTACGTGT CCGGGGAGCT GGAGCACGTC 
ACCCGCCGCA GCCTCAGCAT CGACAAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268919.1
CDS	8..2263
Translation	MNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTVIGSARCKAFTTREGRLAAAYNLVQRGITNLCVIGGDGSLTGADIFRSEWGSLLEELVAAGRISESTAQTYSHLSIVGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSRYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATADTQACVVSLSGNQSVRLPLMECVQVTKEVQKAMDEKRFDEAIQLRGRSFENNWNIYKLLAHQKVSKEKTNFSLAILNVGAPAAGMNAAVRSAVRSGISQGHTVYVVHDGFEGLAKGQVQEVSWHDVAGWLGRGGSMLGTKRTLPRSYMEKIVDNIRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIQDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWMSEKLRAVYRNGRVFANAPDSACVIGLQKKAVAFSPVTELKEVTDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVTRRSLSIDKGF

Target ORF information:

RefSeq Version	XM_022413211.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413211.1

ORF Insert Sequence:

ATGAACGCGG CCGTCAGAGC TGTGACGCGC ATGGGCATTT ACGTGGGAGC CAAGGTCTTC 
CTCATCTACG AGGGCTATGA GGGTCTCGTG GAAGGAGGTG AGAACATCAA GCAGGCCAAC 
TGGCTCAGCG TCTCCAACAT CATCCAGCTG GGCGGCACCG TCATCGGCAG CGCGCGCTGT 
AAGGCCTTCA CCACCCGGGA GGGACGCCTG GCGGCGGCCT ACAACCTGGT GCAGCGCGGC 
ATCACCAACC TGTGCGTCAT CGGCGGGGAC GGCAGCCTGA CGGGCGCCGA CATCTTCCGC 
AGCGAGTGGG GCAGCCTGCT GGAGGAGCTG GTGGCGGCAG GCAGGATCTC GGAGAGCACG 
GCGCAGACCT ACTCGCACCT GAGCATCGTG GGGCTGGTGG GCTCCATCGA CAACGACTTC 
TGCGGCACCG ACATGACCAT CGGCACGGAC TCGGCCCTGC ACCGCATCAT GGAGGTCATC 
GACGCCATCA CCACCACCGC CCAGAGCCAC CAGAGGACCT TCGTGCTGGA GGTGATGGGA 
CGGCACTGCG GGTACCTGGC CCTGGTGTCC GCCCTGGCCT CGGGGGCCGA CTGGCTCTTC 
ATCCCCGAGG CGCCCCCCGA GGACGGCTGG GAGAACTTCA TGTGCGAGAG GCTCGGAGAG 
ACTCGGAGCA GGGGGTCCCG ACTGAACATC ATCATCATCG CGGAGGGCGC CATCGACCGC 
AACGGGAAGC CCATCTCGTC CCGCTACGTG AAGGACCTGG TGGTCCAGAG GCTGGGCTTC 
GACACGCGTG TGACCGTGCT GGGCCACGTG CAGAGGGGAG GGACCCCTTC GGCATTTGAC 
CGCATCCTGA GCAGCAAGAT GGGCATGGAG GCCGTCATGG CGCTGCTGGA GGCCACAGCG 
GACACGCAGG CCTGCGTGGT CAGCCTCTCG GGGAACCAGT CCGTGCGGCT GCCCCTCATG 
GAGTGTGTGC AGGTGACCAA GGAGGTGCAG AAGGCCATGG ACGAGAAGCG GTTCGACGAG 
GCCATCCAGC TCCGCGGCAG GAGCTTTGAG AACAACTGGA ACATTTACAA GCTGCTTGCA 
CACCAGAAGG TCTCCAAGGA GAAGACCAAC TTCTCCTTGG CCATCCTGAA CGTGGGGGCC 
CCGGCGGCTG GCATGAACGC GGCCGTGCGC TCGGCGGTGC GGTCGGGCAT CTCCCAGGGC 
CACACGGTGT ACGTCGTACA CGACGGCTTC GAGGGTCTGG CCAAGGGTCA GGTACAAGAG 
GTGAGTTGGC ACGACGTGGC CGGCTGGCTG GGCCGTGGCG GCTCCATGCT GGGGACGAAG 
AGGACGCTGC CCAGGAGCTA CATGGAGAAG ATAGTGGACA ACATTCGCAC CTACAACATC 
CACGCCCTGC TGGTCATCGG GGGCTTTGAG GCCTACGAGG GGGTGCTGCA GCTGGTGGAG 
GCGCGCGGCC GCTACGAGGA GCTCTGCATC GTCATGTGCG TCATCCCGGC CACCATCAGC 
AACAACGTGC CGGGCACCGA CTTCAGTCTG GGCTCAGACA CCGCCGTCAA CGCCGCCATG 
GAGAGCTGTG ACCGCATCAA GCAGTCGGCG TCGGGCACCA AGCGCCGCGT GTTCATCGTG 
GAGACCATGG GCGGCTACTG TGGCTACCTG GCCACGGTGA CTGGCATTGC TGTGGGGGCC 
GACGCCGCCT ACGTCTTCGA GGACCCTTTC AACATCCAAG ACCTGAAAGC CAACGTGGAG 
CACATGACCG AGAAGATGAA GACAGACATC CAGAGGGGCC TCGTGCTCCG AAACGAGAAG 
TGCCACGAGC ACTACACCAC GGAGTTTCTG TATAACCTGT ACTCCTCCGA GGGGAAGGGC 
GTCTTTGACT GTAGGACCAA CGTTCTGGGC CACCTGCAGC AGGGTGGGGC TCCGACCCCC 
TTTGACCGGA ACTACGGCAC GAAGCTGGGG GTGAAGGCGA TGCTCTGGAT GTCAGAGAAG 
CTGCGGGCCG TCTACCGCAA CGGCCGCGTG TTCGCCAACG CCCCCGATTC GGCCTGCGTG 
ATCGGCCTGC AGAAGAAGGC GGTGGCCTTC AGCCCCGTCA CCGAGCTCAA GGAGGTCACG 
GACTTTGAGC ACCGCATGCC CCGGGAGCAG TGGTGGCTGA ACCTGCGGCT CATGCTGAAG 
ATGCTGGCGC ACTACCGCAT CAGCATGGCC GACTACGTGT CCGGGGAGCT GGAGCACGTC 
ACCCGCCGCA GCCTCAGCAT CGACAAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PFKL ( XM_022413210.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_022268918.1 Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe283930
Clone ID Related Accession (Same CDS sequence)	XM_022413211.1 , XM_022413210.1
Accession Version	XM_022413210.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ATP-dependent 6-phosphofructokinase, liver type isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006613.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCGG CCGTCAGAGC TGTGACGCGC ATGGGCATTT ACGTGGGAGC CAAGGTCTTC 
CTCATCTACG AGGGCTATGA GGGTCTCGTG GAAGGAGGTG AGAACATCAA GCAGGCCAAC 
TGGCTCAGCG TCTCCAACAT CATCCAGCTG GGCGGCACCG TCATCGGCAG CGCGCGCTGT 
AAGGCCTTCA CCACCCGGGA GGGACGCCTG GCGGCGGCCT ACAACCTGGT GCAGCGCGGC 
ATCACCAACC TGTGCGTCAT CGGCGGGGAC GGCAGCCTGA CGGGCGCCGA CATCTTCCGC 
AGCGAGTGGG GCAGCCTGCT GGAGGAGCTG GTGGCGGCAG GCAGGATCTC GGAGAGCACG 
GCGCAGACCT ACTCGCACCT GAGCATCGTG GGGCTGGTGG GCTCCATCGA CAACGACTTC 
TGCGGCACCG ACATGACCAT CGGCACGGAC TCGGCCCTGC ACCGCATCAT GGAGGTCATC 
GACGCCATCA CCACCACCGC CCAGAGCCAC CAGAGGACCT TCGTGCTGGA GGTGATGGGA 
CGGCACTGCG GGTACCTGGC CCTGGTGTCC GCCCTGGCCT CGGGGGCCGA CTGGCTCTTC 
ATCCCCGAGG CGCCCCCCGA GGACGGCTGG GAGAACTTCA TGTGCGAGAG GCTCGGAGAG 
ACTCGGAGCA GGGGGTCCCG ACTGAACATC ATCATCATCG CGGAGGGCGC CATCGACCGC 
AACGGGAAGC CCATCTCGTC CCGCTACGTG AAGGACCTGG TGGTCCAGAG GCTGGGCTTC 
GACACGCGTG TGACCGTGCT GGGCCACGTG CAGAGGGGAG GGACCCCTTC GGCATTTGAC 
CGCATCCTGA GCAGCAAGAT GGGCATGGAG GCCGTCATGG CGCTGCTGGA GGCCACAGCG 
GACACGCAGG CCTGCGTGGT CAGCCTCTCG GGGAACCAGT CCGTGCGGCT GCCCCTCATG 
GAGTGTGTGC AGGTGACCAA GGAGGTGCAG AAGGCCATGG ACGAGAAGCG GTTCGACGAG 
GCCATCCAGC TCCGCGGCAG GAGCTTTGAG AACAACTGGA ACATTTACAA GCTGCTTGCA 
CACCAGAAGG TCTCCAAGGA GAAGACCAAC TTCTCCTTGG CCATCCTGAA CGTGGGGGCC 
CCGGCGGCTG GCATGAACGC GGCCGTGCGC TCGGCGGTGC GGTCGGGCAT CTCCCAGGGC 
CACACGGTGT ACGTCGTACA CGACGGCTTC GAGGGTCTGG CCAAGGGTCA GGTACAAGAG 
GTGAGTTGGC ACGACGTGGC CGGCTGGCTG GGCCGTGGCG GCTCCATGCT GGGGACGAAG 
AGGACGCTGC CCAGGAGCTA CATGGAGAAG ATAGTGGACA ACATTCGCAC CTACAACATC 
CACGCCCTGC TGGTCATCGG GGGCTTTGAG GCCTACGAGG GGGTGCTGCA GCTGGTGGAG 
GCGCGCGGCC GCTACGAGGA GCTCTGCATC GTCATGTGCG TCATCCCGGC CACCATCAGC 
AACAACGTGC CGGGCACCGA CTTCAGTCTG GGCTCAGACA CCGCCGTCAA CGCCGCCATG 
GAGAGCTGTG ACCGCATCAA GCAGTCGGCG TCGGGCACCA AGCGCCGCGT GTTCATCGTG 
GAGACCATGG GCGGCTACTG TGGCTACCTG GCCACGGTGA CTGGCATTGC TGTGGGGGCC 
GACGCCGCCT ACGTCTTCGA GGACCCTTTC AACATCCAAG ACCTGAAAGC CAACGTGGAG 
CACATGACCG AGAAGATGAA GACAGACATC CAGAGGGGCC TCGTGCTCCG AAACGAGAAG 
TGCCACGAGC ACTACACCAC GGAGTTTCTG TATAACCTGT ACTCCTCCGA GGGGAAGGGC 
GTCTTTGACT GTAGGACCAA CGTTCTGGGC CACCTGCAGC AGGGTGGGGC TCCGACCCCC 
TTTGACCGGA ACTACGGCAC GAAGCTGGGG GTGAAGGCGA TGCTCTGGAT GTCAGAGAAG 
CTGCGGGCCG TCTACCGCAA CGGCCGCGTG TTCGCCAACG CCCCCGATTC GGCCTGCGTG 
ATCGGCCTGC AGAAGAAGGC GGTGGCCTTC AGCCCCGTCA CCGAGCTCAA GGAGGTCACG 
GACTTTGAGC ACCGCATGCC CCGGGAGCAG TGGTGGCTGA ACCTGCGGCT CATGCTGAAG 
ATGCTGGCGC ACTACCGCAT CAGCATGGCC GACTACGTGT CCGGGGAGCT GGAGCACGTC 
ACCCGCCGCA GCCTCAGCAT CGACAAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268918.1
CDS	22..2277
Translation	MNAAVRAVTRMGIYVGAKVFLIYEGYEGLVEGGENIKQANWLSVSNIIQLGGTVIGSARCKAFTTREGRLAAAYNLVQRGITNLCVIGGDGSLTGADIFRSEWGSLLEELVAAGRISESTAQTYSHLSIVGLVGSIDNDFCGTDMTIGTDSALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWENFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSRYVKDLVVQRLGFDTRVTVLGHVQRGGTPSAFDRILSSKMGMEAVMALLEATADTQACVVSLSGNQSVRLPLMECVQVTKEVQKAMDEKRFDEAIQLRGRSFENNWNIYKLLAHQKVSKEKTNFSLAILNVGAPAAGMNAAVRSAVRSGISQGHTVYVVHDGFEGLAKGQVQEVSWHDVAGWLGRGGSMLGTKRTLPRSYMEKIVDNIRTYNIHALLVIGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLGSDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFNIQDLKANVEHMTEKMKTDIQRGLVLRNEKCHEHYTTEFLYNLYSSEGKGVFDCRTNVLGHLQQGGAPTPFDRNYGTKLGVKAMLWMSEKLRAVYRNGRVFANAPDSACVIGLQKKAVAFSPVTELKEVTDFEHRMPREQWWLNLRLMLKMLAHYRISMADYVSGELEHVTRRSLSIDKGF

Target ORF information:

RefSeq Version	XM_022413210.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phosphofructokinase, liver type (PFKL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022413210.1

ORF Insert Sequence:

ATGAACGCGG CCGTCAGAGC TGTGACGCGC ATGGGCATTT ACGTGGGAGC CAAGGTCTTC 
CTCATCTACG AGGGCTATGA GGGTCTCGTG GAAGGAGGTG AGAACATCAA GCAGGCCAAC 
TGGCTCAGCG TCTCCAACAT CATCCAGCTG GGCGGCACCG TCATCGGCAG CGCGCGCTGT 
AAGGCCTTCA CCACCCGGGA GGGACGCCTG GCGGCGGCCT ACAACCTGGT GCAGCGCGGC 
ATCACCAACC TGTGCGTCAT CGGCGGGGAC GGCAGCCTGA CGGGCGCCGA CATCTTCCGC 
AGCGAGTGGG GCAGCCTGCT GGAGGAGCTG GTGGCGGCAG GCAGGATCTC GGAGAGCACG 
GCGCAGACCT ACTCGCACCT GAGCATCGTG GGGCTGGTGG GCTCCATCGA CAACGACTTC 
TGCGGCACCG ACATGACCAT CGGCACGGAC TCGGCCCTGC ACCGCATCAT GGAGGTCATC 
GACGCCATCA CCACCACCGC CCAGAGCCAC CAGAGGACCT TCGTGCTGGA GGTGATGGGA 
CGGCACTGCG GGTACCTGGC CCTGGTGTCC GCCCTGGCCT CGGGGGCCGA CTGGCTCTTC 
ATCCCCGAGG CGCCCCCCGA GGACGGCTGG GAGAACTTCA TGTGCGAGAG GCTCGGAGAG 
ACTCGGAGCA GGGGGTCCCG ACTGAACATC ATCATCATCG CGGAGGGCGC CATCGACCGC 
AACGGGAAGC CCATCTCGTC CCGCTACGTG AAGGACCTGG TGGTCCAGAG GCTGGGCTTC 
GACACGCGTG TGACCGTGCT GGGCCACGTG CAGAGGGGAG GGACCCCTTC GGCATTTGAC 
CGCATCCTGA GCAGCAAGAT GGGCATGGAG GCCGTCATGG CGCTGCTGGA GGCCACAGCG 
GACACGCAGG CCTGCGTGGT CAGCCTCTCG GGGAACCAGT CCGTGCGGCT GCCCCTCATG 
GAGTGTGTGC AGGTGACCAA GGAGGTGCAG AAGGCCATGG ACGAGAAGCG GTTCGACGAG 
GCCATCCAGC TCCGCGGCAG GAGCTTTGAG AACAACTGGA ACATTTACAA GCTGCTTGCA 
CACCAGAAGG TCTCCAAGGA GAAGACCAAC TTCTCCTTGG CCATCCTGAA CGTGGGGGCC 
CCGGCGGCTG GCATGAACGC GGCCGTGCGC TCGGCGGTGC GGTCGGGCAT CTCCCAGGGC 
CACACGGTGT ACGTCGTACA CGACGGCTTC GAGGGTCTGG CCAAGGGTCA GGTACAAGAG 
GTGAGTTGGC ACGACGTGGC CGGCTGGCTG GGCCGTGGCG GCTCCATGCT GGGGACGAAG 
AGGACGCTGC CCAGGAGCTA CATGGAGAAG ATAGTGGACA ACATTCGCAC CTACAACATC 
CACGCCCTGC TGGTCATCGG GGGCTTTGAG GCCTACGAGG GGGTGCTGCA GCTGGTGGAG 
GCGCGCGGCC GCTACGAGGA GCTCTGCATC GTCATGTGCG TCATCCCGGC CACCATCAGC 
AACAACGTGC CGGGCACCGA CTTCAGTCTG GGCTCAGACA CCGCCGTCAA CGCCGCCATG 
GAGAGCTGTG ACCGCATCAA GCAGTCGGCG TCGGGCACCA AGCGCCGCGT GTTCATCGTG 
GAGACCATGG GCGGCTACTG TGGCTACCTG GCCACGGTGA CTGGCATTGC TGTGGGGGCC 
GACGCCGCCT ACGTCTTCGA GGACCCTTTC AACATCCAAG ACCTGAAAGC CAACGTGGAG 
CACATGACCG AGAAGATGAA GACAGACATC CAGAGGGGCC TCGTGCTCCG AAACGAGAAG 
TGCCACGAGC ACTACACCAC GGAGTTTCTG TATAACCTGT ACTCCTCCGA GGGGAAGGGC 
GTCTTTGACT GTAGGACCAA CGTTCTGGGC CACCTGCAGC AGGGTGGGGC TCCGACCCCC 
TTTGACCGGA ACTACGGCAC GAAGCTGGGG GTGAAGGCGA TGCTCTGGAT GTCAGAGAAG 
CTGCGGGCCG TCTACCGCAA CGGCCGCGTG TTCGCCAACG CCCCCGATTC GGCCTGCGTG 
ATCGGCCTGC AGAAGAAGGC GGTGGCCTTC AGCCCCGTCA CCGAGCTCAA GGAGGTCACG 
GACTTTGAGC ACCGCATGCC CCGGGAGCAG TGGTGGCTGA ACCTGCGGCT CATGCTGAAG 
ATGCTGGCGC ACTACCGCAT CAGCATGGCC GACTACGTGT CCGGGGAGCT GGAGCACGTC 
ACCCGCCGCA GCCTCAGCAT CGACAAGGGC TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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PFKL cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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サポート

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