UBR1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBR1
Entrez Gene ID	487521
Full Name	ubiquitin protein ligase E3 component n-recognin 1
General protein information	Preferred Names ubiquitin protein ligase E3 component n-recognin 1 Names E3 ubiquitin-protein ligase UBR1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005638320.3	XP_005638377.1	E3 ubiquitin-protein ligase UBR1 isoform X1
XM_544645.5	XP_544645.2	E3 ubiquitin-protein ligase UBR1 isoform X2
XM_014109463.1	XP_013964938.1	E3 ubiquitin-protein ligase UBR1 isoform X3
XM_005638320.2	XP_005638377.1	E3 ubiquitin-protein ligase UBR1 isoform X1
XM_005638321.2	XP_005638378.1	E3 ubiquitin-protein ligase UBR1 isoform X4
XM_544645.6	XP_544645.2	E3 ubiquitin-protein ligase UBR1 isoform X2
XM_022412908.1	XP_022268616.1	E3 ubiquitin-protein ligase UBR1 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBR1	XP_510341.3
	UBR1	XP_001105721.2
Canis lupus familiaris (dog)	UBR1	XP_544645.2
Bos taurus (cattle)	UBR1	NP_001192826.1
Danio rerio (zebrafish)	ubr1	XP_002665264.3
Xenopus tropicalis (tropical clawed frog)	ubr1	XP_002941132.2
Rattus norvegicus (Norway rat)	Ubr1	NP_001171543.1
Homo sapiens (human)	UBR1	NP_777576.1
Mus musculus (house mouse)	Ubr1	NP_033487.2
Gallus gallus (chicken)	UBR1	XP_421165.3
Schizosaccharomyces pombe (fission yeast)	ubr1	NP_596158.2
Magnaporthe oryzae (rice blast fungus)	MGG_13171	XP_003710667.1
Neurospora crassa	NCU03234	XP_965334.2

Gene Ontology

The following UBR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe283783	XM_022412908.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe44254	XM_014109463.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe28745	XM_005638320.3 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe28746	XM_544645.6 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe28747	XM_005638321.2 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe28745	XM_005638320.2	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe28746	XM_544645.5	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBR1 ( XM_022412908.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268616.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X3, mRNA.

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CloneID	OCe283783
Clone ID Related Accession (Same CDS sequence)	XM_022412908.1
Accession Version	XM_022412908.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACTTGGAAAA GCAGGAGGAG AGTATACAAA TGTCCATATT CACTCCACTG 
GAATGGTACT TATTTGGAGA AGATCCAGAT ATTTGCTTAG AGAAATTGAA ACACAGTGGA 
GCATTTCAAC TCTGTGGAAA GGTATTCAAA AGTGGAGAGA CAACCTATTC TTGTAGGGAC 
TGTGCAATTG ACCCAACATG TGTACTCTGT ATGGACTGCT TCCAGAATAG TGTTCATAAA 
AACCATCGTT ACAAGATGCA TACATCTACT GGAGGGGGAT TCTGTGACTG TGGAGACACA 
GAGGCATGGA AAACTGGCCC TTTTTGTGTA AGTCATGAAC CTGGAAGAGC AGATACTACA 
AAAGAGAATT TACGTTGTCC ATTGAATGAA GAGGTAATTG CCCAAGCCCG GAAAATATTC 
CCTTCAGTGA TAAAATACAT TGTAGAAATG ACTATATGGG AGGAGGAAAA AGAACTGCCT 
CCTGAACTCC AAATAAGGGA GAAAAATGAA AGATACTATT GTGTCCTTTT CAATGACGAA 
CACCATTCAT ATGACCATGT CATATACAGT CTACAAAGAG CTCTTGATTG TGAGCTCGCA 
GAGGCCCAGT TGCATACGAC TGCCATAGAC AAAGAGGGCC GTCGGGCTGT CAAAGCAGGT 
GCTTTTGCTG CTTGCCAGGA AGCAAAGGAA GATATAAAGA GCCATTCAGA AAATGTCTCG 
CAACATCCAC TTCATGTAGA AGTATTACAC TCTGAGGTTA TGGCTCATCA GAAATTTGCT 
TTGCGTCTTG GCTCCTGGAT GAACAAAATT ATGAGCTATT CAAGTGACTT TAGACAGATC 
TTTTGCCAAG CATGCCTTAG AGAAGAACCC GGCTCTGAGA ATCCCTGTCT CATAAGCAGA 
TTAATGCTTT GGGATGCAAA GCTTTATAAA GGTGCCCGTA AGATCCTTCA CGAATTGATC 
TTCAGCAGTT TTTTTATGGA GATGGAATAC AAAAAATTCT TTGCTATGGA ATTTGTGAAG 
TATTATAAAC AGCTGCAGAA AGAATATATC AGTGATGATC ATGACAGAAG TATCTCAGTA 
ACTGCACTCT CAGTTCAAAT GTTTACTGTT CCTACTCTGG CTCGACATCT TATTGAAGAG 
CAGAATGTTA TCTCTGTCAT TACTGAAACC CTACTAGAAG TTTTGCCTGA ATACTTGGAC 
AGGAACAATA AATTCAACTT TCAAGGTTAT AGCCAGGACA AATTGGGAAG AGTATATGCA 
GTAATATGTG ATCTAAAGTA TATCCTAATC AGCAAGCCCA CAGTATGGAC AGAAAGATTA 
AGAATGCAAT TCCTTGAAGG TTTTCGATCT TTTTTGAAGA TTCTTACCTG TATGCAGGGA 
ATGGAAGAAA TCAGAAGACA GGTTGGGCAA CACATAGAAG TGGATCCTGA CTGGGAGGCT 
GCCATTGCTA TACAGATGCA ATTAAAGAAT ATCTTACTCA TGTTCCAAGA GTGGTGTGCT 
TGTGATAAAG AACTCTTACT GGTGGCTTAT AAAGAATGTC ACAAAGCTGT GATGAGGTGC 
AGTACAAGTT TTATGTCTAG TAGTAAGACA GTGGTACAAT TGTGTGGTCA TACTCTAGAA 
ACTAAGTCCT ACAAAGTATC TGAAGATCTT GTAAGCATAC ATCTACCACT CTCTAGGACT 
CTTGCTGGTC TTCATGTGCG TTTAAGCAGG CTGGGAGCTG TTTCAAGACT GCATGAATTT 
GTGCCTTTTG AGGACTTTCA AGTAGAGGTA CTGGTGGAAT ATCCTTTGCG TTGTCTGGTA 
TTGGTTGCCC AGGTTGTTGC TGAGATGTGG CGAAGAAATG GGCTCTCTCT CATTAGCCAG 
GTGTTTTATT ACCAAGATGT TAAGTGCAGA GAAGAAATGT ATGATAAAGA TATCATTATG 
CTTCAGATTG GTGCATCTTT AATGGATCCT AATAAGTTTT TGTTACTGAT ACTTCAGAGG 
TATGAACTTG CTGATGCTTT TAATAAGACA ATATCCACAA AAGACCAGGA TTTGATTAAA 
CAATATAATA CATTAATAGA AGAAATGCTT CAAGTCCTCA TCTATATTGT GGGAGAGCGT 
TATGTACCTG GAGTGGGAAA TGTGACCAAG GAAGAGGTCA CAATGAGAGA AATTATTCAC 
CTGCTTTGTA TTGAACCCAT GCCACACAGC GCCATTGCCA AAAATTTACC TGAGAATGAA 
AATAATGAAA CCGGCCTAGA GAACGTCATT AACAAAGTGG CCACATTTAA GAAACCAGGT 
GTGTCAGGCC ATGGAGTTTA TGAACTGAAA GATGAATCAT TGAAAGACTT CAATATGTAC 
TTTTATCATT ATTCCAAAAC ACAGCATAGC AAGGCAGAAC ATATGCAGAA AAAAAGGAGA 
AAACAAGAAA ACAAAGATGA AGCATTACCA CCACCACCAC CTCCTGAATT CTGCCCTGCT 
TTCAGCAAAG TGGTTAACCT TCTCAATTGT GATATCATGA TGTACATTCT CAGGACCATA 
TTTGAGCGGG CAATTGACAC AGATTCTAAC TTGTGGACTG AAGGGATGCT TCAGATGGCA 
TTTCATCTTC TGGCATTGGG TTTACTAGAA GAAAAGCAAC AGCTTCAAAA AGCTCCTGAA 
GAAGAAGTGA CATTTGACTT TTACCATAAG GCTTCAAGAT TGGGAAGTTC AGCCATGAAT 
GCTCAGAATA TACAAATGCT TTTGGAAAAA CTCAAAGGAA TTCCTCAGTT AGAAGGCCAA 
AAGGACATGA TAACATGGAT ACTCCAGATG TTTGACACAG TGAAACGATT AAGAGAAAAA 
TCTTGTTTAA TTGTAGCCAC TACATCAGGA TCAGAATCTA TTAAGAATGA TGAGATTACT 
CATGATAAAG AAAAAGCAGA ACGGAAAAGA AAAGCTGAAG CTGCTAGATT GCACCGCCAG 
AAGATCATGG CTCAGATGTC TGCCTTACAG AAAAATTTCA TTGAAACTCA CAAACTCATG 
TATGATAGCA CCTCAGAAAT GTCTGGGAAA GAAGACTCCA TTATGGAGGA AGAGAGCACC 
CCAGCAGTCA GTGACTACTC TAGAATTGCT TTGGGTCCTA AACGGGGTCC GACTGTTACT 
GAAAAGGAGG TGCTGACATG CATCCTCTGC CAAGAAGAAC AAGAGGTGAA AATAGAAAAT 
AATGCCATGG TATTATCGGC CTGTGTCCAG AAATCCACCG CCTTAACCCA GCACAGGGGA 
AAACCTATAG AACTCTCAGG GGAAACTGTG GACCCACTCT TCATGGATCC AGACTTGGCA 
TATGGAACTT ACACAGGAAG CTGTGGTCAT GTAATGCATG CGGTGTGCTG GCAGAAGTAT 
TTTGAAGCTG TACAGCTGAG CTCTCAGCAG CGCATTCATG TTGACCTTTT TGATTTGGAG 
AGTGGAGAAT ATCTTTGCCC TCTTTGCAAG TCTCTGTGCA ATACTGTGAT CCCCATTATT 
CCTTTGCAAC CTCAAAGGAT AAACAGTGAG AATGCAGAAG CTCTTGCTCA ACTTTTGACC 
CTGGCACAGT GGATACAGAC TGTTCTGGCC AGAATATCAG GTTATAATAT GAAACATGCC 
AAAGGAGAAA ACCCAACAAT TCCAGCTTTA TTTGATCAAG GAATGGGCGA TTCCACTTTT 
GAGTTCCATT CTATCCTCAG CTTTGGAATT CAGCCTTCGA TAAAATATTC TAATAGCATC 
AAGGAAATGG TCATTCTCTT TGCTACAACG ATTTATAGAA TTGGACTGAA AGTACCTCCT 
GATGAAATGG ATCCTCGAAT CCCCATGATG ACCTGGAGCA CGTGTGCTTT CACTGTCCAG 
GCAATTGAAA ACCTCTTGGG AGATGAAGGA AAACCTCTGT TTGGAGCACT TCAAAATAGA 
CAGCATAATG GTCTGAAAGC ATTAATGCAG TTTGCCATTG CACAGAAGAT TACCTGTCCT 
CAGGTCCTGA TACAGAAACA CCTGATTCGT CTCCTCTCAG TTGTTCTTCC TAACCTAAAA 
TCAGAAGATA CACCTTGTCT CCTATCTATA GATCTCTTTC ATGTTTTGGT TGGTGCTGTG 
TTAGCATTCC CATCCTTGTA TTGGGATGAC ACTGTTGATC TGCAGCCTTC ATCAATTAGT 
TCTTCCTATA ACCACCTTTA TCTCTTCCAT TTGATCACCA TGGCACACAT GCTTCAGATC 
CTTCTTACCA TAGACACAGA CATTCCCCTT GCTCAGGTTC AAGAAGAGAG TGAAGAGGCT 
CGTTCTGCAT CTTCTTTCTT GGCAGAAGTT TCTCAGTATA CAAGTGGCTG CATTGGGTGT 
GGTGTTCCTG GCTGGTATTT GTGGGTCTCA CTGAAGAATG GTATCACCCC TTACCTTCGC 
TGTGCTGCAC TGTTTTTCCA CTATTTGCTT GGAGTAACTC CACCTGAAGA ACTGCTTACC 
AATCCTGCAG AAGGAGAGTA CAGTGCACTC TGTAGCTATC TGTCTTTACC AACAAATTTG 
TTCCTGCTTT TTCAAGAATA TTGGGATACT GTAAGACCCC TCCTCCAGAG GTGGTGTGCA 
GATCCTGCCT TACTAAATTG TTTGAAGCAA AAAAGCACCA TAGTCAGATA CCCTAGAAAA 
AGAAATAGTT TGATAGAGCT TCCTGATGAC TATAGCTGCC TCCTAAATCA AGCTTCTCAT 
TTCAGGTGCC CACGGTCTGC AGATGATGAG CGAAAGCACC CTGTTCTGTG TCTTTTCTGT 
GGAGCTATAC TATGTTCTCA GAACATTTGC TGCCAAGAAA TTGTGAATGG GGAAGAGGTC 
GGAGCTTGCA TTTTTCATGC ACTTCACTGT GGAGCTGGAG TCTGCATTTT CCTTAAAATC 
CGAGAATGCA GAGTAGTCCT GGTTGAAGGT AAAGCCAGAG GCTGTGCCTA TCCAGCCCCT 
TACTTGGATG AATATGGAGA AACAGACCCT GGCCTGAAGA GGGGCAACCC TCTTCATTTA 
TCTCGTGAGC GGTATCGGAA GCTCCATTTG GTGTGGCAAC AGCACTGCAT TATAGAAGAG 
ATTGCTAGGA GCCAAGAAAC TAATCAGATG TTATTTGGAT TCAACTGGCA GTTATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268616.1
CDS	263..5362
Translation	MDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGCIGCGVPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELLTNPAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKSTIVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_022412908.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412908.1

ORF Insert Sequence:

ATGGACCCAG ACTTGGAAAA GCAGGAGGAG AGTATACAAA TGTCCATATT CACTCCACTG 
GAATGGTACT TATTTGGAGA AGATCCAGAT ATTTGCTTAG AGAAATTGAA ACACAGTGGA 
GCATTTCAAC TCTGTGGAAA GGTATTCAAA AGTGGAGAGA CAACCTATTC TTGTAGGGAC 
TGTGCAATTG ACCCAACATG TGTACTCTGT ATGGACTGCT TCCAGAATAG TGTTCATAAA 
AACCATCGTT ACAAGATGCA TACATCTACT GGAGGGGGAT TCTGTGACTG TGGAGACACA 
GAGGCATGGA AAACTGGCCC TTTTTGTGTA AGTCATGAAC CTGGAAGAGC AGATACTACA 
AAAGAGAATT TACGTTGTCC ATTGAATGAA GAGGTAATTG CCCAAGCCCG GAAAATATTC 
CCTTCAGTGA TAAAATACAT TGTAGAAATG ACTATATGGG AGGAGGAAAA AGAACTGCCT 
CCTGAACTCC AAATAAGGGA GAAAAATGAA AGATACTATT GTGTCCTTTT CAATGACGAA 
CACCATTCAT ATGACCATGT CATATACAGT CTACAAAGAG CTCTTGATTG TGAGCTCGCA 
GAGGCCCAGT TGCATACGAC TGCCATAGAC AAAGAGGGCC GTCGGGCTGT CAAAGCAGGT 
GCTTTTGCTG CTTGCCAGGA AGCAAAGGAA GATATAAAGA GCCATTCAGA AAATGTCTCG 
CAACATCCAC TTCATGTAGA AGTATTACAC TCTGAGGTTA TGGCTCATCA GAAATTTGCT 
TTGCGTCTTG GCTCCTGGAT GAACAAAATT ATGAGCTATT CAAGTGACTT TAGACAGATC 
TTTTGCCAAG CATGCCTTAG AGAAGAACCC GGCTCTGAGA ATCCCTGTCT CATAAGCAGA 
TTAATGCTTT GGGATGCAAA GCTTTATAAA GGTGCCCGTA AGATCCTTCA CGAATTGATC 
TTCAGCAGTT TTTTTATGGA GATGGAATAC AAAAAATTCT TTGCTATGGA ATTTGTGAAG 
TATTATAAAC AGCTGCAGAA AGAATATATC AGTGATGATC ATGACAGAAG TATCTCAGTA 
ACTGCACTCT CAGTTCAAAT GTTTACTGTT CCTACTCTGG CTCGACATCT TATTGAAGAG 
CAGAATGTTA TCTCTGTCAT TACTGAAACC CTACTAGAAG TTTTGCCTGA ATACTTGGAC 
AGGAACAATA AATTCAACTT TCAAGGTTAT AGCCAGGACA AATTGGGAAG AGTATATGCA 
GTAATATGTG ATCTAAAGTA TATCCTAATC AGCAAGCCCA CAGTATGGAC AGAAAGATTA 
AGAATGCAAT TCCTTGAAGG TTTTCGATCT TTTTTGAAGA TTCTTACCTG TATGCAGGGA 
ATGGAAGAAA TCAGAAGACA GGTTGGGCAA CACATAGAAG TGGATCCTGA CTGGGAGGCT 
GCCATTGCTA TACAGATGCA ATTAAAGAAT ATCTTACTCA TGTTCCAAGA GTGGTGTGCT 
TGTGATAAAG AACTCTTACT GGTGGCTTAT AAAGAATGTC ACAAAGCTGT GATGAGGTGC 
AGTACAAGTT TTATGTCTAG TAGTAAGACA GTGGTACAAT TGTGTGGTCA TACTCTAGAA 
ACTAAGTCCT ACAAAGTATC TGAAGATCTT GTAAGCATAC ATCTACCACT CTCTAGGACT 
CTTGCTGGTC TTCATGTGCG TTTAAGCAGG CTGGGAGCTG TTTCAAGACT GCATGAATTT 
GTGCCTTTTG AGGACTTTCA AGTAGAGGTA CTGGTGGAAT ATCCTTTGCG TTGTCTGGTA 
TTGGTTGCCC AGGTTGTTGC TGAGATGTGG CGAAGAAATG GGCTCTCTCT CATTAGCCAG 
GTGTTTTATT ACCAAGATGT TAAGTGCAGA GAAGAAATGT ATGATAAAGA TATCATTATG 
CTTCAGATTG GTGCATCTTT AATGGATCCT AATAAGTTTT TGTTACTGAT ACTTCAGAGG 
TATGAACTTG CTGATGCTTT TAATAAGACA ATATCCACAA AAGACCAGGA TTTGATTAAA 
CAATATAATA CATTAATAGA AGAAATGCTT CAAGTCCTCA TCTATATTGT GGGAGAGCGT 
TATGTACCTG GAGTGGGAAA TGTGACCAAG GAAGAGGTCA CAATGAGAGA AATTATTCAC 
CTGCTTTGTA TTGAACCCAT GCCACACAGC GCCATTGCCA AAAATTTACC TGAGAATGAA 
AATAATGAAA CCGGCCTAGA GAACGTCATT AACAAAGTGG CCACATTTAA GAAACCAGGT 
GTGTCAGGCC ATGGAGTTTA TGAACTGAAA GATGAATCAT TGAAAGACTT CAATATGTAC 
TTTTATCATT ATTCCAAAAC ACAGCATAGC AAGGCAGAAC ATATGCAGAA AAAAAGGAGA 
AAACAAGAAA ACAAAGATGA AGCATTACCA CCACCACCAC CTCCTGAATT CTGCCCTGCT 
TTCAGCAAAG TGGTTAACCT TCTCAATTGT GATATCATGA TGTACATTCT CAGGACCATA 
TTTGAGCGGG CAATTGACAC AGATTCTAAC TTGTGGACTG AAGGGATGCT TCAGATGGCA 
TTTCATCTTC TGGCATTGGG TTTACTAGAA GAAAAGCAAC AGCTTCAAAA AGCTCCTGAA 
GAAGAAGTGA CATTTGACTT TTACCATAAG GCTTCAAGAT TGGGAAGTTC AGCCATGAAT 
GCTCAGAATA TACAAATGCT TTTGGAAAAA CTCAAAGGAA TTCCTCAGTT AGAAGGCCAA 
AAGGACATGA TAACATGGAT ACTCCAGATG TTTGACACAG TGAAACGATT AAGAGAAAAA 
TCTTGTTTAA TTGTAGCCAC TACATCAGGA TCAGAATCTA TTAAGAATGA TGAGATTACT 
CATGATAAAG AAAAAGCAGA ACGGAAAAGA AAAGCTGAAG CTGCTAGATT GCACCGCCAG 
AAGATCATGG CTCAGATGTC TGCCTTACAG AAAAATTTCA TTGAAACTCA CAAACTCATG 
TATGATAGCA CCTCAGAAAT GTCTGGGAAA GAAGACTCCA TTATGGAGGA AGAGAGCACC 
CCAGCAGTCA GTGACTACTC TAGAATTGCT TTGGGTCCTA AACGGGGTCC GACTGTTACT 
GAAAAGGAGG TGCTGACATG CATCCTCTGC CAAGAAGAAC AAGAGGTGAA AATAGAAAAT 
AATGCCATGG TATTATCGGC CTGTGTCCAG AAATCCACCG CCTTAACCCA GCACAGGGGA 
AAACCTATAG AACTCTCAGG GGAAACTGTG GACCCACTCT TCATGGATCC AGACTTGGCA 
TATGGAACTT ACACAGGAAG CTGTGGTCAT GTAATGCATG CGGTGTGCTG GCAGAAGTAT 
TTTGAAGCTG TACAGCTGAG CTCTCAGCAG CGCATTCATG TTGACCTTTT TGATTTGGAG 
AGTGGAGAAT ATCTTTGCCC TCTTTGCAAG TCTCTGTGCA ATACTGTGAT CCCCATTATT 
CCTTTGCAAC CTCAAAGGAT AAACAGTGAG AATGCAGAAG CTCTTGCTCA ACTTTTGACC 
CTGGCACAGT GGATACAGAC TGTTCTGGCC AGAATATCAG GTTATAATAT GAAACATGCC 
AAAGGAGAAA ACCCAACAAT TCCAGCTTTA TTTGATCAAG GAATGGGCGA TTCCACTTTT 
GAGTTCCATT CTATCCTCAG CTTTGGAATT CAGCCTTCGA TAAAATATTC TAATAGCATC 
AAGGAAATGG TCATTCTCTT TGCTACAACG ATTTATAGAA TTGGACTGAA AGTACCTCCT 
GATGAAATGG ATCCTCGAAT CCCCATGATG ACCTGGAGCA CGTGTGCTTT CACTGTCCAG 
GCAATTGAAA ACCTCTTGGG AGATGAAGGA AAACCTCTGT TTGGAGCACT TCAAAATAGA 
CAGCATAATG GTCTGAAAGC ATTAATGCAG TTTGCCATTG CACAGAAGAT TACCTGTCCT 
CAGGTCCTGA TACAGAAACA CCTGATTCGT CTCCTCTCAG TTGTTCTTCC TAACCTAAAA 
TCAGAAGATA CACCTTGTCT CCTATCTATA GATCTCTTTC ATGTTTTGGT TGGTGCTGTG 
TTAGCATTCC CATCCTTGTA TTGGGATGAC ACTGTTGATC TGCAGCCTTC ATCAATTAGT 
TCTTCCTATA ACCACCTTTA TCTCTTCCAT TTGATCACCA TGGCACACAT GCTTCAGATC 
CTTCTTACCA TAGACACAGA CATTCCCCTT GCTCAGGTTC AAGAAGAGAG TGAAGAGGCT 
CGTTCTGCAT CTTCTTTCTT GGCAGAAGTT TCTCAGTATA CAAGTGGCTG CATTGGGTGT 
GGTGTTCCTG GCTGGTATTT GTGGGTCTCA CTGAAGAATG GTATCACCCC TTACCTTCGC 
TGTGCTGCAC TGTTTTTCCA CTATTTGCTT GGAGTAACTC CACCTGAAGA ACTGCTTACC 
AATCCTGCAG AAGGAGAGTA CAGTGCACTC TGTAGCTATC TGTCTTTACC AACAAATTTG 
TTCCTGCTTT TTCAAGAATA TTGGGATACT GTAAGACCCC TCCTCCAGAG GTGGTGTGCA 
GATCCTGCCT TACTAAATTG TTTGAAGCAA AAAAGCACCA TAGTCAGATA CCCTAGAAAA 
AGAAATAGTT TGATAGAGCT TCCTGATGAC TATAGCTGCC TCCTAAATCA AGCTTCTCAT 
TTCAGGTGCC CACGGTCTGC AGATGATGAG CGAAAGCACC CTGTTCTGTG TCTTTTCTGT 
GGAGCTATAC TATGTTCTCA GAACATTTGC TGCCAAGAAA TTGTGAATGG GGAAGAGGTC 
GGAGCTTGCA TTTTTCATGC ACTTCACTGT GGAGCTGGAG TCTGCATTTT CCTTAAAATC 
CGAGAATGCA GAGTAGTCCT GGTTGAAGGT AAAGCCAGAG GCTGTGCCTA TCCAGCCCCT 
TACTTGGATG AATATGGAGA AACAGACCCT GGCCTGAAGA GGGGCAACCC TCTTCATTTA 
TCTCGTGAGC GGTATCGGAA GCTCCATTTG GTGTGGCAAC AGCACTGCAT TATAGAAGAG 
ATTGCTAGGA GCCAAGAAAC TAATCAGATG TTATTTGGAT TCAACTGGCA GTTATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( XM_014109463.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013964938.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X3, mRNA.

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CloneID	OCe44254
Clone ID Related Accession (Same CDS sequence)	XM_014109463.1
Accession Version	XM_014109463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GCTGCATTGG GTGTGGTGTT 
CCTGGCTGGT ATTTGTGGGT CTCACTGAAG AATGGTATCA CCCCTTACCT TCGCTGTGCT 
GCACTGTTTT TCCACTATTT GCTTGGAGTA ACTCCACCTG AAGAACTGCT TACCAATCCT 
GCAGAAGGAG AGTACAGTGC ACTCTGTAGC TATCTGTCTT TACCAACAAA TTTGTTCCTG 
CTTTTTCAAG AATATTGGGA TACTGTAAGA CCCCTCCTCC AGAGGTGCCC ACGGTCTGCA 
GATGATGAGC GAAAGCACCC TGTTCTGTGT CTTTTCTGTG GAGCTATACT ATGTTCTCAG 
AACATTTGCT GCCAAGAAAT TGTGAATGGG GAAGAGGTCG GAGCTTGCAT TTTTCATGCA 
CTTCACTGTG GAGCTGGAGT CTGCATTTTC CTTAAAATCC GAGAATGCAG AGTAGTCCTG 
GTTGAAGGTA AAGCCAGAGG CTGTGCCTAT CCAGCCCCTT ACTTGGATGA ATATGGAGAA 
ACAGACCCTG GCCTGAAGAG GGGCAACCCT CTTCATTTAT CTCGTGAGCG GTATCGGAAG 
CTCCATTTGG TGTGGCAACA GCACTGCATT ATAGAAGAGA TTGCTAGGAG CCAAGAAACT 
AATCAGATGT TATTTGGATT CAACTGGCAG TTATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964938.1
CDS	104..5362
Translation	MADEEAGGAERMEVSTELPQTPQLLASSCSWVCGENKLLVYAYLRKVGVRRLQVTSDIIRTLWKKKKRTLWWWDQQVDFYTTFLHHLAQLVPEIYSAEMDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGCIGCGVPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELLTNPAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_014109463.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109463.1

ORF Insert Sequence:

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GCTGCATTGG GTGTGGTGTT 
CCTGGCTGGT ATTTGTGGGT CTCACTGAAG AATGGTATCA CCCCTTACCT TCGCTGTGCT 
GCACTGTTTT TCCACTATTT GCTTGGAGTA ACTCCACCTG AAGAACTGCT TACCAATCCT 
GCAGAAGGAG AGTACAGTGC ACTCTGTAGC TATCTGTCTT TACCAACAAA TTTGTTCCTG 
CTTTTTCAAG AATATTGGGA TACTGTAAGA CCCCTCCTCC AGAGGTGCCC ACGGTCTGCA 
GATGATGAGC GAAAGCACCC TGTTCTGTGT CTTTTCTGTG GAGCTATACT ATGTTCTCAG 
AACATTTGCT GCCAAGAAAT TGTGAATGGG GAAGAGGTCG GAGCTTGCAT TTTTCATGCA 
CTTCACTGTG GAGCTGGAGT CTGCATTTTC CTTAAAATCC GAGAATGCAG AGTAGTCCTG 
GTTGAAGGTA AAGCCAGAGG CTGTGCCTAT CCAGCCCCTT ACTTGGATGA ATATGGAGAA 
ACAGACCCTG GCCTGAAGAG GGGCAACCCT CTTCATTTAT CTCGTGAGCG GTATCGGAAG 
CTCCATTTGG TGTGGCAACA GCACTGCATT ATAGAAGAGA TTGCTAGGAG CCAAGAAACT 
AATCAGATGT TATTTGGATT CAACTGGCAG TTATTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( XM_005638320.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638377.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe28745
Clone ID Related Accession (Same CDS sequence)	XM_005638320.3 , XM_005638320.2
Accession Version	XM_005638320.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638320.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GCTGCATTGG GTGTGGTGTT 
CCTGGCTGGT ATTTGTGGGT CTCACTGAAG AATGGTATCA CCCCTTACCT TCGCTGTGCT 
GCACTGTTTT TCCACTATTT GCTTGGAGTA ACTCCACCTG AAGAACTGCT TACCAATCCT 
GCAGAAGGAG AGTACAGTGC ACTCTGTAGC TATCTGTCTT TACCAACAAA TTTGTTCCTG 
CTTTTTCAAG AATATTGGGA TACTGTAAGA CCCCTCCTCC AGAGGTGGTG TGCAGATCCT 
GCCTTACTAA ATTGTTTGAA GCAAAAAAGC ACCATAGTCA GATACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA TGACTATAGC TGCCTCCTAA ATCAAGCTTC TCATTTCAGG 
TGCCCACGGT CTGCAGATGA TGAGCGAAAG CACCCTGTTC TGTGTCTTTT CTGTGGAGCT 
ATACTATGTT CTCAGAACAT TTGCTGCCAA GAAATTGTGA ATGGGGAAGA GGTCGGAGCT 
TGCATTTTTC ATGCACTTCA CTGTGGAGCT GGAGTCTGCA TTTTCCTTAA AATCCGAGAA 
TGCAGAGTAG TCCTGGTTGA AGGTAAAGCC AGAGGCTGTG CCTATCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCTGGCCTG AAGAGGGGCA ACCCTCTTCA TTTATCTCGT 
GAGCGGTATC GGAAGCTCCA TTTGGTGTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
AGGAGCCAAG AAACTAATCA GATGTTATTT GGATTCAACT GGCAGTTATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638377.1
CDS	46..5439
Translation	MADEEAGGAERMEVSTELPQTPQLLASSCSWVCGENKLLVYAYLRKVGVRRLQVTSDIIRTLWKKKKRTLWWWDQQVDFYTTFLHHLAQLVPEIYSAEMDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGCIGCGVPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELLTNPAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKSTIVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_005638320.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638320.3

ORF Insert Sequence:

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GCTGCATTGG GTGTGGTGTT 
CCTGGCTGGT ATTTGTGGGT CTCACTGAAG AATGGTATCA CCCCTTACCT TCGCTGTGCT 
GCACTGTTTT TCCACTATTT GCTTGGAGTA ACTCCACCTG AAGAACTGCT TACCAATCCT 
GCAGAAGGAG AGTACAGTGC ACTCTGTAGC TATCTGTCTT TACCAACAAA TTTGTTCCTG 
CTTTTTCAAG AATATTGGGA TACTGTAAGA CCCCTCCTCC AGAGGTGGTG TGCAGATCCT 
GCCTTACTAA ATTGTTTGAA GCAAAAAAGC ACCATAGTCA GATACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA TGACTATAGC TGCCTCCTAA ATCAAGCTTC TCATTTCAGG 
TGCCCACGGT CTGCAGATGA TGAGCGAAAG CACCCTGTTC TGTGTCTTTT CTGTGGAGCT 
ATACTATGTT CTCAGAACAT TTGCTGCCAA GAAATTGTGA ATGGGGAAGA GGTCGGAGCT 
TGCATTTTTC ATGCACTTCA CTGTGGAGCT GGAGTCTGCA TTTTCCTTAA AATCCGAGAA 
TGCAGAGTAG TCCTGGTTGA AGGTAAAGCC AGAGGCTGTG CCTATCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCTGGCCTG AAGAGGGGCA ACCCTCTTCA TTTATCTCGT 
GAGCGGTATC GGAAGCTCCA TTTGGTGTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
AGGAGCCAAG AAACTAATCA GATGTTATTT GGATTCAACT GGCAGTTATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( XM_005638320.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638377.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X1, mRNA.

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CloneID	OCe28745
Clone ID Related Accession (Same CDS sequence)	XM_005638320.3 , XM_005638320.2
Accession Version	XM_005638320.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638320.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GCTGCATTGG GTGTGGTGTT 
CCTGGCTGGT ATTTGTGGGT CTCACTGAAG AATGGTATCA CCCCTTACCT TCGCTGTGCT 
GCACTGTTTT TCCACTATTT GCTTGGAGTA ACTCCACCTG AAGAACTGCT TACCAATCCT 
GCAGAAGGAG AGTACAGTGC ACTCTGTAGC TATCTGTCTT TACCAACAAA TTTGTTCCTG 
CTTTTTCAAG AATATTGGGA TACTGTAAGA CCCCTCCTCC AGAGGTGGTG TGCAGATCCT 
GCCTTACTAA ATTGTTTGAA GCAAAAAAGC ACCATAGTCA GATACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA TGACTATAGC TGCCTCCTAA ATCAAGCTTC TCATTTCAGG 
TGCCCACGGT CTGCAGATGA TGAGCGAAAG CACCCTGTTC TGTGTCTTTT CTGTGGAGCT 
ATACTATGTT CTCAGAACAT TTGCTGCCAA GAAATTGTGA ATGGGGAAGA GGTCGGAGCT 
TGCATTTTTC ATGCACTTCA CTGTGGAGCT GGAGTCTGCA TTTTCCTTAA AATCCGAGAA 
TGCAGAGTAG TCCTGGTTGA AGGTAAAGCC AGAGGCTGTG CCTATCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCTGGCCTG AAGAGGGGCA ACCCTCTTCA TTTATCTCGT 
GAGCGGTATC GGAAGCTCCA TTTGGTGTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
AGGAGCCAAG AAACTAATCA GATGTTATTT GGATTCAACT GGCAGTTATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638377.1
CDS	104..5497
Translation	MADEEAGGAERMEVSTELPQTPQLLASSCSWVCGENKLLVYAYLRKVGVRRLQVTSDIIRTLWKKKKRTLWWWDQQVDFYTTFLHHLAQLVPEIYSAEMDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGCIGCGVPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELLTNPAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKSTIVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_005638320.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638320.2

ORF Insert Sequence:

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GCTGCATTGG GTGTGGTGTT 
CCTGGCTGGT ATTTGTGGGT CTCACTGAAG AATGGTATCA CCCCTTACCT TCGCTGTGCT 
GCACTGTTTT TCCACTATTT GCTTGGAGTA ACTCCACCTG AAGAACTGCT TACCAATCCT 
GCAGAAGGAG AGTACAGTGC ACTCTGTAGC TATCTGTCTT TACCAACAAA TTTGTTCCTG 
CTTTTTCAAG AATATTGGGA TACTGTAAGA CCCCTCCTCC AGAGGTGGTG TGCAGATCCT 
GCCTTACTAA ATTGTTTGAA GCAAAAAAGC ACCATAGTCA GATACCCTAG AAAAAGAAAT 
AGTTTGATAG AGCTTCCTGA TGACTATAGC TGCCTCCTAA ATCAAGCTTC TCATTTCAGG 
TGCCCACGGT CTGCAGATGA TGAGCGAAAG CACCCTGTTC TGTGTCTTTT CTGTGGAGCT 
ATACTATGTT CTCAGAACAT TTGCTGCCAA GAAATTGTGA ATGGGGAAGA GGTCGGAGCT 
TGCATTTTTC ATGCACTTCA CTGTGGAGCT GGAGTCTGCA TTTTCCTTAA AATCCGAGAA 
TGCAGAGTAG TCCTGGTTGA AGGTAAAGCC AGAGGCTGTG CCTATCCAGC CCCTTACTTG 
GATGAATATG GAGAAACAGA CCCTGGCCTG AAGAGGGGCA ACCCTCTTCA TTTATCTCGT 
GAGCGGTATC GGAAGCTCCA TTTGGTGTGG CAACAGCACT GCATTATAGA AGAGATTGCT 
AGGAGCCAAG AAACTAATCA GATGTTATTT GGATTCAACT GGCAGTTATT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( XM_544645.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_544645.2 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X2, mRNA.

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CloneID	OCe28746
Clone ID Related Accession (Same CDS sequence)	XM_544645.5 , XM_544645.6
Accession Version	XM_544645.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_544645.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TTGGTGGGAT CAGCAAGTAG ATTTTTATAC TACTTTCTTG 
CACCATTTGG CACAGTTGGT GCCAGAAATT TATTCTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AGAGTATACA AATGTCCATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAACACAGTG GAGCATTTCA ACTCTGTGGA 
AAGGTATTCA AAAGTGGAGA GACAACCTAT TCTTGTAGGG ACTGTGCAAT TGACCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGAAT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACATCTA CTGGAGGGGG ATTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAGTCATGA ACCTGGAAGA GCAGATACTA CAAAAGAGAA TTTACGTTGT 
CCATTGAATG AAGAGGTAAT TGCCCAAGCC CGGAAAATAT TCCCTTCAGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAGGAGGAA AAAGAACTGC CTCCTGAACT CCAAATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGACG AACACCATTC ATATGACCAT 
GTCATATACA GTCTACAAAG AGCTCTTGAT TGTGAGCTCG CAGAGGCCCA GTTGCATACG 
ACTGCCATAG ACAAAGAGGG CCGTCGGGCT GTCAAAGCAG GTGCTTTTGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGCCATTCA GAAAATGTCT CGCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCTGAGGT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CCGGCTCTGA GAATCCCTGT CTCATAAGCA GATTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CACGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAATT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCAG TAACTGCACT CTCAGTTCAA 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CCCTACTAGA AGTTTTGCCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTTCAAGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGATCTAAAG 
TATATCCTAA TCAGCAAGCC CACAGTATGG ACAGAAAGAT TAAGAATGCA ATTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAGGTTGGGC AACACATAGA AGTGGATCCT GACTGGGAGG CTGCCATTGC TATACAGATG 
CAATTAAAGA ATATCTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATAA AGAACTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACAAG TTTTATGTCT 
AGTAGTAAGA CAGTGGTACA ATTGTGTGGT CATACTCTAG AAACTAAGTC CTACAAAGTA 
TCTGAAGATC TTGTAAGCAT ACATCTACCA CTCTCTAGGA CTCTTGCTGG TCTTCATGTG 
CGTTTAAGCA GGCTGGGAGC TGTTTCAAGA CTGCATGAAT TTGTGCCTTT TGAGGACTTT 
CAAGTAGAGG TACTGGTGGA ATATCCTTTG CGTTGTCTGG TATTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGGCTCTCT CTCATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATTA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CTAATAAGTT TTTGTTACTG ATACTTCAGA GGTATGAACT TGCTGATGCT 
TTTAATAAGA CAATATCCAC AAAAGACCAG GATTTGATTA AACAATATAA TACATTAATA 
GAAGAAATGC TTCAAGTCCT CATCTATATT GTGGGAGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AGGAAGAGGT CACAATGAGA GAAATTATTC ACCTGCTTTG TATTGAACCC 
ATGCCACACA GCGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACCGGCCTA 
GAGAACGTCA TTAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCAGG CCATGGAGTT 
TATGAACTGA AAGATGAATC ATTGAAAGAC TTCAATATGT ACTTTTATCA TTATTCCAAA 
ACACAGCATA GCAAGGCAGA ACATATGCAG AAAAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTAC CACCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGGTTAAC 
CTTCTCAATT GTGATATCAT GATGTACATT CTCAGGACCA TATTTGAGCG GGCAATTGAC 
ACAGATTCTA ACTTGTGGAC TGAAGGGATG CTTCAGATGG CATTTCATCT TCTGGCATTG 
GGTTTACTAG AAGAAAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT GACATTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTTTTGGAAA AACTCAAAGG AATTCCTCAG TTAGAAGGCC AAAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAACGA TTAAGAGAAA AATCTTGTTT AATTGTAGCC 
ACTACATCAG GATCAGAATC TATTAAGAAT GATGAGATTA CTCATGATAA AGAAAAAGCA 
GAACGGAAAA GAAAAGCTGA AGCTGCTAGA TTGCACCGCC AGAAGATCAT GGCTCAGATG 
TCTGCCTTAC AGAAAAATTT CATTGAAACT CACAAACTCA TGTATGATAG CACCTCAGAA 
ATGTCTGGGA AAGAAGACTC CATTATGGAG GAAGAGAGCA CCCCAGCAGT CAGTGACTAC 
TCTAGAATTG CTTTGGGTCC TAAACGGGGT CCGACTGTTA CTGAAAAGGA GGTGCTGACA 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAAATAGAAA ATAATGCCAT GGTATTATCG 
GCCTGTGTCC AGAAATCCAC CGCCTTAACC CAGCACAGGG GAAAACCTAT AGAACTCTCA 
GGGGAAACTG TGGACCCACT CTTCATGGAT CCAGACTTGG CATATGGAAC TTACACAGGA 
AGCTGTGGTC ATGTAATGCA TGCGGTGTGC TGGCAGAAGT ATTTTGAAGC TGTACAGCTG 
AGCTCTCAGC AGCGCATTCA TGTTGACCTT TTTGATTTGG AGAGTGGAGA ATATCTTTGC 
CCTCTTTGCA AGTCTCTGTG CAATACTGTG ATCCCCATTA TTCCTTTGCA ACCTCAAAGG 
ATAAACAGTG AGAATGCAGA AGCTCTTGCT CAACTTTTGA CCCTGGCACA GTGGATACAG 
ACTGTTCTGG CCAGAATATC AGGTTATAAT ATGAAACATG CCAAAGGAGA AAACCCAACA 
ATTCCAGCTT TATTTGATCA AGGAATGGGC GATTCCACTT TTGAGTTCCA TTCTATCCTC 
AGCTTTGGAA TTCAGCCTTC GATAAAATAT TCTAATAGCA TCAAGGAAAT GGTCATTCTC 
TTTGCTACAA CGATTTATAG AATTGGACTG AAAGTACCTC CTGATGAAAT GGATCCTCGA 
ATCCCCATGA TGACCTGGAG CACGTGTGCT TTCACTGTCC AGGCAATTGA AAACCTCTTG 
GGAGATGAAG GAAAACCTCT GTTTGGAGCA CTTCAAAATA GACAGCATAA TGGTCTGAAA 
GCATTAATGC AGTTTGCCAT TGCACAGAAG ATTACCTGTC CTCAGGTCCT GATACAGAAA 
CACCTGATTC GTCTCCTCTC AGTTGTTCTT CCTAACCTAA AATCAGAAGA TACACCTTGT 
CTCCTATCTA TAGATCTCTT TCATGTTTTG GTTGGTGCTG TGTTAGCATT CCCATCCTTG 
TATTGGGATG ACACTGTTGA TCTGCAGCCT TCATCAATTA GTTCTTCCTA TAACCACCTT 
TATCTCTTCC ATTTGATCAC CATGGCACAC ATGCTTCAGA TCCTTCTTAC CATAGACACA 
GACATTCCCC TTGCTCAGGT TCAAGAAGAG AGTGAAGAGG CTCGTTCTGC ATCTTCTTTC 
TTGGCAGAAG TTTCTCAGTA TACAAGTGGC TGCATTGGGT GTGGTGTTCC TGGCTGGTAT 
TTGTGGGTCT CACTGAAGAA TGGTATCACC CCTTACCTTC GCTGTGCTGC ACTGTTTTTC 
CACTATTTGC TTGGAGTAAC TCCACCTGAA GAACTGCTTA CCAATCCTGC AGAAGGAGAG 
TACAGTGCAC TCTGTAGCTA TCTGTCTTTA CCAACAAATT TGTTCCTGCT TTTTCAAGAA 
TATTGGGATA CTGTAAGACC CCTCCTCCAG AGGTGGTGTG CAGATCCTGC CTTACTAAAT 
TGTTTGAAGC AAAAAAGCAC CATAGTCAGA TACCCTAGAA AAAGAAATAG TTTGATAGAG 
CTTCCTGATG ACTATAGCTG CCTCCTAAAT CAAGCTTCTC ATTTCAGGTG CCCACGGTCT 
GCAGATGATG AGCGAAAGCA CCCTGTTCTG TGTCTTTTCT GTGGAGCTAT ACTATGTTCT 
CAGAACATTT GCTGCCAAGA AATTGTGAAT GGGGAAGAGG TCGGAGCTTG CATTTTTCAT 
GCACTTCACT GTGGAGCTGG AGTCTGCATT TTCCTTAAAA TCCGAGAATG CAGAGTAGTC 
CTGGTTGAAG GTAAAGCCAG AGGCTGTGCC TATCCAGCCC CTTACTTGGA TGAATATGGA 
GAAACAGACC CTGGCCTGAA GAGGGGCAAC CCTCTTCATT TATCTCGTGA GCGGTATCGG 
AAGCTCCATT TGGTGTGGCA ACAGCACTGC ATTATAGAAG AGATTGCTAG GAGCCAAGAA 
ACTAATCAGA TGTTATTTGG ATTCAACTGG CAGTTATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544645.2
CDS	1..5262
Translation	MADEEAGGAERMEVSTELPQTPQLLASWWDQQVDFYTTFLHHLAQLVPEIYSAEMDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGCIGCGVPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELLTNPAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKSTIVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_544645.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544645.5

ORF Insert Sequence:

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TTGGTGGGAT CAGCAAGTAG ATTTTTATAC TACTTTCTTG 
CACCATTTGG CACAGTTGGT GCCAGAAATT TATTCTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AGAGTATACA AATGTCCATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAACACAGTG GAGCATTTCA ACTCTGTGGA 
AAGGTATTCA AAAGTGGAGA GACAACCTAT TCTTGTAGGG ACTGTGCAAT TGACCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGAAT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACATCTA CTGGAGGGGG ATTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAGTCATGA ACCTGGAAGA GCAGATACTA CAAAAGAGAA TTTACGTTGT 
CCATTGAATG AAGAGGTAAT TGCCCAAGCC CGGAAAATAT TCCCTTCAGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAGGAGGAA AAAGAACTGC CTCCTGAACT CCAAATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGACG AACACCATTC ATATGACCAT 
GTCATATACA GTCTACAAAG AGCTCTTGAT TGTGAGCTCG CAGAGGCCCA GTTGCATACG 
ACTGCCATAG ACAAAGAGGG CCGTCGGGCT GTCAAAGCAG GTGCTTTTGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGCCATTCA GAAAATGTCT CGCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCTGAGGT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CCGGCTCTGA GAATCCCTGT CTCATAAGCA GATTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CACGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAATT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCAG TAACTGCACT CTCAGTTCAA 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CCCTACTAGA AGTTTTGCCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTTCAAGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGATCTAAAG 
TATATCCTAA TCAGCAAGCC CACAGTATGG ACAGAAAGAT TAAGAATGCA ATTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAGGTTGGGC AACACATAGA AGTGGATCCT GACTGGGAGG CTGCCATTGC TATACAGATG 
CAATTAAAGA ATATCTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATAA AGAACTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACAAG TTTTATGTCT 
AGTAGTAAGA CAGTGGTACA ATTGTGTGGT CATACTCTAG AAACTAAGTC CTACAAAGTA 
TCTGAAGATC TTGTAAGCAT ACATCTACCA CTCTCTAGGA CTCTTGCTGG TCTTCATGTG 
CGTTTAAGCA GGCTGGGAGC TGTTTCAAGA CTGCATGAAT TTGTGCCTTT TGAGGACTTT 
CAAGTAGAGG TACTGGTGGA ATATCCTTTG CGTTGTCTGG TATTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGGCTCTCT CTCATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATTA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CTAATAAGTT TTTGTTACTG ATACTTCAGA GGTATGAACT TGCTGATGCT 
TTTAATAAGA CAATATCCAC AAAAGACCAG GATTTGATTA AACAATATAA TACATTAATA 
GAAGAAATGC TTCAAGTCCT CATCTATATT GTGGGAGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AGGAAGAGGT CACAATGAGA GAAATTATTC ACCTGCTTTG TATTGAACCC 
ATGCCACACA GCGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACCGGCCTA 
GAGAACGTCA TTAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCAGG CCATGGAGTT 
TATGAACTGA AAGATGAATC ATTGAAAGAC TTCAATATGT ACTTTTATCA TTATTCCAAA 
ACACAGCATA GCAAGGCAGA ACATATGCAG AAAAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTAC CACCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGGTTAAC 
CTTCTCAATT GTGATATCAT GATGTACATT CTCAGGACCA TATTTGAGCG GGCAATTGAC 
ACAGATTCTA ACTTGTGGAC TGAAGGGATG CTTCAGATGG CATTTCATCT TCTGGCATTG 
GGTTTACTAG AAGAAAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT GACATTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTTTTGGAAA AACTCAAAGG AATTCCTCAG TTAGAAGGCC AAAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAACGA TTAAGAGAAA AATCTTGTTT AATTGTAGCC 
ACTACATCAG GATCAGAATC TATTAAGAAT GATGAGATTA CTCATGATAA AGAAAAAGCA 
GAACGGAAAA GAAAAGCTGA AGCTGCTAGA TTGCACCGCC AGAAGATCAT GGCTCAGATG 
TCTGCCTTAC AGAAAAATTT CATTGAAACT CACAAACTCA TGTATGATAG CACCTCAGAA 
ATGTCTGGGA AAGAAGACTC CATTATGGAG GAAGAGAGCA CCCCAGCAGT CAGTGACTAC 
TCTAGAATTG CTTTGGGTCC TAAACGGGGT CCGACTGTTA CTGAAAAGGA GGTGCTGACA 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAAATAGAAA ATAATGCCAT GGTATTATCG 
GCCTGTGTCC AGAAATCCAC CGCCTTAACC CAGCACAGGG GAAAACCTAT AGAACTCTCA 
GGGGAAACTG TGGACCCACT CTTCATGGAT CCAGACTTGG CATATGGAAC TTACACAGGA 
AGCTGTGGTC ATGTAATGCA TGCGGTGTGC TGGCAGAAGT ATTTTGAAGC TGTACAGCTG 
AGCTCTCAGC AGCGCATTCA TGTTGACCTT TTTGATTTGG AGAGTGGAGA ATATCTTTGC 
CCTCTTTGCA AGTCTCTGTG CAATACTGTG ATCCCCATTA TTCCTTTGCA ACCTCAAAGG 
ATAAACAGTG AGAATGCAGA AGCTCTTGCT CAACTTTTGA CCCTGGCACA GTGGATACAG 
ACTGTTCTGG CCAGAATATC AGGTTATAAT ATGAAACATG CCAAAGGAGA AAACCCAACA 
ATTCCAGCTT TATTTGATCA AGGAATGGGC GATTCCACTT TTGAGTTCCA TTCTATCCTC 
AGCTTTGGAA TTCAGCCTTC GATAAAATAT TCTAATAGCA TCAAGGAAAT GGTCATTCTC 
TTTGCTACAA CGATTTATAG AATTGGACTG AAAGTACCTC CTGATGAAAT GGATCCTCGA 
ATCCCCATGA TGACCTGGAG CACGTGTGCT TTCACTGTCC AGGCAATTGA AAACCTCTTG 
GGAGATGAAG GAAAACCTCT GTTTGGAGCA CTTCAAAATA GACAGCATAA TGGTCTGAAA 
GCATTAATGC AGTTTGCCAT TGCACAGAAG ATTACCTGTC CTCAGGTCCT GATACAGAAA 
CACCTGATTC GTCTCCTCTC AGTTGTTCTT CCTAACCTAA AATCAGAAGA TACACCTTGT 
CTCCTATCTA TAGATCTCTT TCATGTTTTG GTTGGTGCTG TGTTAGCATT CCCATCCTTG 
TATTGGGATG ACACTGTTGA TCTGCAGCCT TCATCAATTA GTTCTTCCTA TAACCACCTT 
TATCTCTTCC ATTTGATCAC CATGGCACAC ATGCTTCAGA TCCTTCTTAC CATAGACACA 
GACATTCCCC TTGCTCAGGT TCAAGAAGAG AGTGAAGAGG CTCGTTCTGC ATCTTCTTTC 
TTGGCAGAAG TTTCTCAGTA TACAAGTGGC TGCATTGGGT GTGGTGTTCC TGGCTGGTAT 
TTGTGGGTCT CACTGAAGAA TGGTATCACC CCTTACCTTC GCTGTGCTGC ACTGTTTTTC 
CACTATTTGC TTGGAGTAAC TCCACCTGAA GAACTGCTTA CCAATCCTGC AGAAGGAGAG 
TACAGTGCAC TCTGTAGCTA TCTGTCTTTA CCAACAAATT TGTTCCTGCT TTTTCAAGAA 
TATTGGGATA CTGTAAGACC CCTCCTCCAG AGGTGGTGTG CAGATCCTGC CTTACTAAAT 
TGTTTGAAGC AAAAAAGCAC CATAGTCAGA TACCCTAGAA AAAGAAATAG TTTGATAGAG 
CTTCCTGATG ACTATAGCTG CCTCCTAAAT CAAGCTTCTC ATTTCAGGTG CCCACGGTCT 
GCAGATGATG AGCGAAAGCA CCCTGTTCTG TGTCTTTTCT GTGGAGCTAT ACTATGTTCT 
CAGAACATTT GCTGCCAAGA AATTGTGAAT GGGGAAGAGG TCGGAGCTTG CATTTTTCAT 
GCACTTCACT GTGGAGCTGG AGTCTGCATT TTCCTTAAAA TCCGAGAATG CAGAGTAGTC 
CTGGTTGAAG GTAAAGCCAG AGGCTGTGCC TATCCAGCCC CTTACTTGGA TGAATATGGA 
GAAACAGACC CTGGCCTGAA GAGGGGCAAC CCTCTTCATT TATCTCGTGA GCGGTATCGG 
AAGCTCCATT TGGTGTGGCA ACAGCACTGC ATTATAGAAG AGATTGCTAG GAGCCAAGAA 
ACTAATCAGA TGTTATTTGG ATTCAACTGG CAGTTATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( XM_544645.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_544645.2 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X2, mRNA.

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CloneID	OCe28746
Clone ID Related Accession (Same CDS sequence)	XM_544645.5 , XM_544645.6
Accession Version	XM_544645.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_544645.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TTGGTGGGAT CAGCAAGTAG ATTTTTATAC TACTTTCTTG 
CACCATTTGG CACAGTTGGT GCCAGAAATT TATTCTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AGAGTATACA AATGTCCATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAACACAGTG GAGCATTTCA ACTCTGTGGA 
AAGGTATTCA AAAGTGGAGA GACAACCTAT TCTTGTAGGG ACTGTGCAAT TGACCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGAAT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACATCTA CTGGAGGGGG ATTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAGTCATGA ACCTGGAAGA GCAGATACTA CAAAAGAGAA TTTACGTTGT 
CCATTGAATG AAGAGGTAAT TGCCCAAGCC CGGAAAATAT TCCCTTCAGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAGGAGGAA AAAGAACTGC CTCCTGAACT CCAAATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGACG AACACCATTC ATATGACCAT 
GTCATATACA GTCTACAAAG AGCTCTTGAT TGTGAGCTCG CAGAGGCCCA GTTGCATACG 
ACTGCCATAG ACAAAGAGGG CCGTCGGGCT GTCAAAGCAG GTGCTTTTGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGCCATTCA GAAAATGTCT CGCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCTGAGGT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CCGGCTCTGA GAATCCCTGT CTCATAAGCA GATTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CACGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAATT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCAG TAACTGCACT CTCAGTTCAA 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CCCTACTAGA AGTTTTGCCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTTCAAGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGATCTAAAG 
TATATCCTAA TCAGCAAGCC CACAGTATGG ACAGAAAGAT TAAGAATGCA ATTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAGGTTGGGC AACACATAGA AGTGGATCCT GACTGGGAGG CTGCCATTGC TATACAGATG 
CAATTAAAGA ATATCTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATAA AGAACTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACAAG TTTTATGTCT 
AGTAGTAAGA CAGTGGTACA ATTGTGTGGT CATACTCTAG AAACTAAGTC CTACAAAGTA 
TCTGAAGATC TTGTAAGCAT ACATCTACCA CTCTCTAGGA CTCTTGCTGG TCTTCATGTG 
CGTTTAAGCA GGCTGGGAGC TGTTTCAAGA CTGCATGAAT TTGTGCCTTT TGAGGACTTT 
CAAGTAGAGG TACTGGTGGA ATATCCTTTG CGTTGTCTGG TATTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGGCTCTCT CTCATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATTA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CTAATAAGTT TTTGTTACTG ATACTTCAGA GGTATGAACT TGCTGATGCT 
TTTAATAAGA CAATATCCAC AAAAGACCAG GATTTGATTA AACAATATAA TACATTAATA 
GAAGAAATGC TTCAAGTCCT CATCTATATT GTGGGAGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AGGAAGAGGT CACAATGAGA GAAATTATTC ACCTGCTTTG TATTGAACCC 
ATGCCACACA GCGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACCGGCCTA 
GAGAACGTCA TTAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCAGG CCATGGAGTT 
TATGAACTGA AAGATGAATC ATTGAAAGAC TTCAATATGT ACTTTTATCA TTATTCCAAA 
ACACAGCATA GCAAGGCAGA ACATATGCAG AAAAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTAC CACCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGGTTAAC 
CTTCTCAATT GTGATATCAT GATGTACATT CTCAGGACCA TATTTGAGCG GGCAATTGAC 
ACAGATTCTA ACTTGTGGAC TGAAGGGATG CTTCAGATGG CATTTCATCT TCTGGCATTG 
GGTTTACTAG AAGAAAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT GACATTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTTTTGGAAA AACTCAAAGG AATTCCTCAG TTAGAAGGCC AAAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAACGA TTAAGAGAAA AATCTTGTTT AATTGTAGCC 
ACTACATCAG GATCAGAATC TATTAAGAAT GATGAGATTA CTCATGATAA AGAAAAAGCA 
GAACGGAAAA GAAAAGCTGA AGCTGCTAGA TTGCACCGCC AGAAGATCAT GGCTCAGATG 
TCTGCCTTAC AGAAAAATTT CATTGAAACT CACAAACTCA TGTATGATAG CACCTCAGAA 
ATGTCTGGGA AAGAAGACTC CATTATGGAG GAAGAGAGCA CCCCAGCAGT CAGTGACTAC 
TCTAGAATTG CTTTGGGTCC TAAACGGGGT CCGACTGTTA CTGAAAAGGA GGTGCTGACA 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAAATAGAAA ATAATGCCAT GGTATTATCG 
GCCTGTGTCC AGAAATCCAC CGCCTTAACC CAGCACAGGG GAAAACCTAT AGAACTCTCA 
GGGGAAACTG TGGACCCACT CTTCATGGAT CCAGACTTGG CATATGGAAC TTACACAGGA 
AGCTGTGGTC ATGTAATGCA TGCGGTGTGC TGGCAGAAGT ATTTTGAAGC TGTACAGCTG 
AGCTCTCAGC AGCGCATTCA TGTTGACCTT TTTGATTTGG AGAGTGGAGA ATATCTTTGC 
CCTCTTTGCA AGTCTCTGTG CAATACTGTG ATCCCCATTA TTCCTTTGCA ACCTCAAAGG 
ATAAACAGTG AGAATGCAGA AGCTCTTGCT CAACTTTTGA CCCTGGCACA GTGGATACAG 
ACTGTTCTGG CCAGAATATC AGGTTATAAT ATGAAACATG CCAAAGGAGA AAACCCAACA 
ATTCCAGCTT TATTTGATCA AGGAATGGGC GATTCCACTT TTGAGTTCCA TTCTATCCTC 
AGCTTTGGAA TTCAGCCTTC GATAAAATAT TCTAATAGCA TCAAGGAAAT GGTCATTCTC 
TTTGCTACAA CGATTTATAG AATTGGACTG AAAGTACCTC CTGATGAAAT GGATCCTCGA 
ATCCCCATGA TGACCTGGAG CACGTGTGCT TTCACTGTCC AGGCAATTGA AAACCTCTTG 
GGAGATGAAG GAAAACCTCT GTTTGGAGCA CTTCAAAATA GACAGCATAA TGGTCTGAAA 
GCATTAATGC AGTTTGCCAT TGCACAGAAG ATTACCTGTC CTCAGGTCCT GATACAGAAA 
CACCTGATTC GTCTCCTCTC AGTTGTTCTT CCTAACCTAA AATCAGAAGA TACACCTTGT 
CTCCTATCTA TAGATCTCTT TCATGTTTTG GTTGGTGCTG TGTTAGCATT CCCATCCTTG 
TATTGGGATG ACACTGTTGA TCTGCAGCCT TCATCAATTA GTTCTTCCTA TAACCACCTT 
TATCTCTTCC ATTTGATCAC CATGGCACAC ATGCTTCAGA TCCTTCTTAC CATAGACACA 
GACATTCCCC TTGCTCAGGT TCAAGAAGAG AGTGAAGAGG CTCGTTCTGC ATCTTCTTTC 
TTGGCAGAAG TTTCTCAGTA TACAAGTGGC TGCATTGGGT GTGGTGTTCC TGGCTGGTAT 
TTGTGGGTCT CACTGAAGAA TGGTATCACC CCTTACCTTC GCTGTGCTGC ACTGTTTTTC 
CACTATTTGC TTGGAGTAAC TCCACCTGAA GAACTGCTTA CCAATCCTGC AGAAGGAGAG 
TACAGTGCAC TCTGTAGCTA TCTGTCTTTA CCAACAAATT TGTTCCTGCT TTTTCAAGAA 
TATTGGGATA CTGTAAGACC CCTCCTCCAG AGGTGGTGTG CAGATCCTGC CTTACTAAAT 
TGTTTGAAGC AAAAAAGCAC CATAGTCAGA TACCCTAGAA AAAGAAATAG TTTGATAGAG 
CTTCCTGATG ACTATAGCTG CCTCCTAAAT CAAGCTTCTC ATTTCAGGTG CCCACGGTCT 
GCAGATGATG AGCGAAAGCA CCCTGTTCTG TGTCTTTTCT GTGGAGCTAT ACTATGTTCT 
CAGAACATTT GCTGCCAAGA AATTGTGAAT GGGGAAGAGG TCGGAGCTTG CATTTTTCAT 
GCACTTCACT GTGGAGCTGG AGTCTGCATT TTCCTTAAAA TCCGAGAATG CAGAGTAGTC 
CTGGTTGAAG GTAAAGCCAG AGGCTGTGCC TATCCAGCCC CTTACTTGGA TGAATATGGA 
GAAACAGACC CTGGCCTGAA GAGGGGCAAC CCTCTTCATT TATCTCGTGA GCGGTATCGG 
AAGCTCCATT TGGTGTGGCA ACAGCACTGC ATTATAGAAG AGATTGCTAG GAGCCAAGAA 
ACTAATCAGA TGTTATTTGG ATTCAACTGG CAGTTATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544645.2
CDS	43..5304
Translation	MADEEAGGAERMEVSTELPQTPQLLASWWDQQVDFYTTFLHHLAQLVPEIYSAEMDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGCIGCGVPGWYLWVSLKNGITPYLRCAALFFHYLLGVTPPEELLTNPAEGEYSALCSYLSLPTNLFLLFQEYWDTVRPLLQRWCADPALLNCLKQKSTIVRYPRKRNSLIELPDDYSCLLNQASHFRCPRSADDERKHPVLCLFCGAILCSQNICCQEIVNGEEVGACIFHALHCGAGVCIFLKIRECRVVLVEGKARGCAYPAPYLDEYGETDPGLKRGNPLHLSRERYRKLHLVWQQHCIIEEIARSQETNQMLFGFNWQLL

Target ORF information:

RefSeq Version	XM_544645.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544645.6

ORF Insert Sequence:

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TTGGTGGGAT CAGCAAGTAG ATTTTTATAC TACTTTCTTG 
CACCATTTGG CACAGTTGGT GCCAGAAATT TATTCTGCTG AAATGGACCC AGACTTGGAA 
AAGCAGGAGG AGAGTATACA AATGTCCATA TTCACTCCAC TGGAATGGTA CTTATTTGGA 
GAAGATCCAG ATATTTGCTT AGAGAAATTG AAACACAGTG GAGCATTTCA ACTCTGTGGA 
AAGGTATTCA AAAGTGGAGA GACAACCTAT TCTTGTAGGG ACTGTGCAAT TGACCCAACA 
TGTGTACTCT GTATGGACTG CTTCCAGAAT AGTGTTCATA AAAACCATCG TTACAAGATG 
CATACATCTA CTGGAGGGGG ATTCTGTGAC TGTGGAGACA CAGAGGCATG GAAAACTGGC 
CCTTTTTGTG TAAGTCATGA ACCTGGAAGA GCAGATACTA CAAAAGAGAA TTTACGTTGT 
CCATTGAATG AAGAGGTAAT TGCCCAAGCC CGGAAAATAT TCCCTTCAGT GATAAAATAC 
ATTGTAGAAA TGACTATATG GGAGGAGGAA AAAGAACTGC CTCCTGAACT CCAAATAAGG 
GAGAAAAATG AAAGATACTA TTGTGTCCTT TTCAATGACG AACACCATTC ATATGACCAT 
GTCATATACA GTCTACAAAG AGCTCTTGAT TGTGAGCTCG CAGAGGCCCA GTTGCATACG 
ACTGCCATAG ACAAAGAGGG CCGTCGGGCT GTCAAAGCAG GTGCTTTTGC TGCTTGCCAG 
GAAGCAAAGG AAGATATAAA GAGCCATTCA GAAAATGTCT CGCAACATCC ACTTCATGTA 
GAAGTATTAC ACTCTGAGGT TATGGCTCAT CAGAAATTTG CTTTGCGTCT TGGCTCCTGG 
ATGAACAAAA TTATGAGCTA TTCAAGTGAC TTTAGACAGA TCTTTTGCCA AGCATGCCTT 
AGAGAAGAAC CCGGCTCTGA GAATCCCTGT CTCATAAGCA GATTAATGCT TTGGGATGCA 
AAGCTTTATA AAGGTGCCCG TAAGATCCTT CACGAATTGA TCTTCAGCAG TTTTTTTATG 
GAGATGGAAT ACAAAAAATT CTTTGCTATG GAATTTGTGA AGTATTATAA ACAGCTGCAG 
AAAGAATATA TCAGTGATGA TCATGACAGA AGTATCTCAG TAACTGCACT CTCAGTTCAA 
ATGTTTACTG TTCCTACTCT GGCTCGACAT CTTATTGAAG AGCAGAATGT TATCTCTGTC 
ATTACTGAAA CCCTACTAGA AGTTTTGCCT GAATACTTGG ACAGGAACAA TAAATTCAAC 
TTTCAAGGTT ATAGCCAGGA CAAATTGGGA AGAGTATATG CAGTAATATG TGATCTAAAG 
TATATCCTAA TCAGCAAGCC CACAGTATGG ACAGAAAGAT TAAGAATGCA ATTCCTTGAA 
GGTTTTCGAT CTTTTTTGAA GATTCTTACC TGTATGCAGG GAATGGAAGA AATCAGAAGA 
CAGGTTGGGC AACACATAGA AGTGGATCCT GACTGGGAGG CTGCCATTGC TATACAGATG 
CAATTAAAGA ATATCTTACT CATGTTCCAA GAGTGGTGTG CTTGTGATAA AGAACTCTTA 
CTGGTGGCTT ATAAAGAATG TCACAAAGCT GTGATGAGGT GCAGTACAAG TTTTATGTCT 
AGTAGTAAGA CAGTGGTACA ATTGTGTGGT CATACTCTAG AAACTAAGTC CTACAAAGTA 
TCTGAAGATC TTGTAAGCAT ACATCTACCA CTCTCTAGGA CTCTTGCTGG TCTTCATGTG 
CGTTTAAGCA GGCTGGGAGC TGTTTCAAGA CTGCATGAAT TTGTGCCTTT TGAGGACTTT 
CAAGTAGAGG TACTGGTGGA ATATCCTTTG CGTTGTCTGG TATTGGTTGC CCAGGTTGTT 
GCTGAGATGT GGCGAAGAAA TGGGCTCTCT CTCATTAGCC AGGTGTTTTA TTACCAAGAT 
GTTAAGTGCA GAGAAGAAAT GTATGATAAA GATATCATTA TGCTTCAGAT TGGTGCATCT 
TTAATGGATC CTAATAAGTT TTTGTTACTG ATACTTCAGA GGTATGAACT TGCTGATGCT 
TTTAATAAGA CAATATCCAC AAAAGACCAG GATTTGATTA AACAATATAA TACATTAATA 
GAAGAAATGC TTCAAGTCCT CATCTATATT GTGGGAGAGC GTTATGTACC TGGAGTGGGA 
AATGTGACCA AGGAAGAGGT CACAATGAGA GAAATTATTC ACCTGCTTTG TATTGAACCC 
ATGCCACACA GCGCCATTGC CAAAAATTTA CCTGAGAATG AAAATAATGA AACCGGCCTA 
GAGAACGTCA TTAACAAAGT GGCCACATTT AAGAAACCAG GTGTGTCAGG CCATGGAGTT 
TATGAACTGA AAGATGAATC ATTGAAAGAC TTCAATATGT ACTTTTATCA TTATTCCAAA 
ACACAGCATA GCAAGGCAGA ACATATGCAG AAAAAAAGGA GAAAACAAGA AAACAAAGAT 
GAAGCATTAC CACCACCACC ACCTCCTGAA TTCTGCCCTG CTTTCAGCAA AGTGGTTAAC 
CTTCTCAATT GTGATATCAT GATGTACATT CTCAGGACCA TATTTGAGCG GGCAATTGAC 
ACAGATTCTA ACTTGTGGAC TGAAGGGATG CTTCAGATGG CATTTCATCT TCTGGCATTG 
GGTTTACTAG AAGAAAAGCA ACAGCTTCAA AAAGCTCCTG AAGAAGAAGT GACATTTGAC 
TTTTACCATA AGGCTTCAAG ATTGGGAAGT TCAGCCATGA ATGCTCAGAA TATACAAATG 
CTTTTGGAAA AACTCAAAGG AATTCCTCAG TTAGAAGGCC AAAAGGACAT GATAACATGG 
ATACTCCAGA TGTTTGACAC AGTGAAACGA TTAAGAGAAA AATCTTGTTT AATTGTAGCC 
ACTACATCAG GATCAGAATC TATTAAGAAT GATGAGATTA CTCATGATAA AGAAAAAGCA 
GAACGGAAAA GAAAAGCTGA AGCTGCTAGA TTGCACCGCC AGAAGATCAT GGCTCAGATG 
TCTGCCTTAC AGAAAAATTT CATTGAAACT CACAAACTCA TGTATGATAG CACCTCAGAA 
ATGTCTGGGA AAGAAGACTC CATTATGGAG GAAGAGAGCA CCCCAGCAGT CAGTGACTAC 
TCTAGAATTG CTTTGGGTCC TAAACGGGGT CCGACTGTTA CTGAAAAGGA GGTGCTGACA 
TGCATCCTCT GCCAAGAAGA ACAAGAGGTG AAAATAGAAA ATAATGCCAT GGTATTATCG 
GCCTGTGTCC AGAAATCCAC CGCCTTAACC CAGCACAGGG GAAAACCTAT AGAACTCTCA 
GGGGAAACTG TGGACCCACT CTTCATGGAT CCAGACTTGG CATATGGAAC TTACACAGGA 
AGCTGTGGTC ATGTAATGCA TGCGGTGTGC TGGCAGAAGT ATTTTGAAGC TGTACAGCTG 
AGCTCTCAGC AGCGCATTCA TGTTGACCTT TTTGATTTGG AGAGTGGAGA ATATCTTTGC 
CCTCTTTGCA AGTCTCTGTG CAATACTGTG ATCCCCATTA TTCCTTTGCA ACCTCAAAGG 
ATAAACAGTG AGAATGCAGA AGCTCTTGCT CAACTTTTGA CCCTGGCACA GTGGATACAG 
ACTGTTCTGG CCAGAATATC AGGTTATAAT ATGAAACATG CCAAAGGAGA AAACCCAACA 
ATTCCAGCTT TATTTGATCA AGGAATGGGC GATTCCACTT TTGAGTTCCA TTCTATCCTC 
AGCTTTGGAA TTCAGCCTTC GATAAAATAT TCTAATAGCA TCAAGGAAAT GGTCATTCTC 
TTTGCTACAA CGATTTATAG AATTGGACTG AAAGTACCTC CTGATGAAAT GGATCCTCGA 
ATCCCCATGA TGACCTGGAG CACGTGTGCT TTCACTGTCC AGGCAATTGA AAACCTCTTG 
GGAGATGAAG GAAAACCTCT GTTTGGAGCA CTTCAAAATA GACAGCATAA TGGTCTGAAA 
GCATTAATGC AGTTTGCCAT TGCACAGAAG ATTACCTGTC CTCAGGTCCT GATACAGAAA 
CACCTGATTC GTCTCCTCTC AGTTGTTCTT CCTAACCTAA AATCAGAAGA TACACCTTGT 
CTCCTATCTA TAGATCTCTT TCATGTTTTG GTTGGTGCTG TGTTAGCATT CCCATCCTTG 
TATTGGGATG ACACTGTTGA TCTGCAGCCT TCATCAATTA GTTCTTCCTA TAACCACCTT 
TATCTCTTCC ATTTGATCAC CATGGCACAC ATGCTTCAGA TCCTTCTTAC CATAGACACA 
GACATTCCCC TTGCTCAGGT TCAAGAAGAG AGTGAAGAGG CTCGTTCTGC ATCTTCTTTC 
TTGGCAGAAG TTTCTCAGTA TACAAGTGGC TGCATTGGGT GTGGTGTTCC TGGCTGGTAT 
TTGTGGGTCT CACTGAAGAA TGGTATCACC CCTTACCTTC GCTGTGCTGC ACTGTTTTTC 
CACTATTTGC TTGGAGTAAC TCCACCTGAA GAACTGCTTA CCAATCCTGC AGAAGGAGAG 
TACAGTGCAC TCTGTAGCTA TCTGTCTTTA CCAACAAATT TGTTCCTGCT TTTTCAAGAA 
TATTGGGATA CTGTAAGACC CCTCCTCCAG AGGTGGTGTG CAGATCCTGC CTTACTAAAT 
TGTTTGAAGC AAAAAAGCAC CATAGTCAGA TACCCTAGAA AAAGAAATAG TTTGATAGAG 
CTTCCTGATG ACTATAGCTG CCTCCTAAAT CAAGCTTCTC ATTTCAGGTG CCCACGGTCT 
GCAGATGATG AGCGAAAGCA CCCTGTTCTG TGTCTTTTCT GTGGAGCTAT ACTATGTTCT 
CAGAACATTT GCTGCCAAGA AATTGTGAAT GGGGAAGAGG TCGGAGCTTG CATTTTTCAT 
GCACTTCACT GTGGAGCTGG AGTCTGCATT TTCCTTAAAA TCCGAGAATG CAGAGTAGTC 
CTGGTTGAAG GTAAAGCCAG AGGCTGTGCC TATCCAGCCC CTTACTTGGA TGAATATGGA 
GAAACAGACC CTGGCCTGAA GAGGGGCAAC CCTCTTCATT TATCTCGTGA GCGGTATCGG 
AAGCTCCATT TGGTGTGGCA ACAGCACTGC ATTATAGAAG AGATTGCTAG GAGCCAAGAA 
ACTAATCAGA TGTTATTTGG ATTCAACTGG CAGTTATTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBR1 ( XM_005638321.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638378.1 Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X4, mRNA.

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CloneID	OCe28747
Clone ID Related Accession (Same CDS sequence)	XM_005638321.2
Accession Version	XM_005638321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4608bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase UBR1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638321.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638378.1
CDS	107..4714
Translation	MADEEAGGAERMEVSTELPQTPQLLASSCSWVCGENKLLVYAYLRKVGVRRLQVTSDIIRTLWKKKKRTLWWWDQQVDFYTTFLHHLAQLVPEIYSAEMDPDLEKQEESIQMSIFTPLEWYLFGEDPDICLEKLKHSGAFQLCGKVFKSGETTYSCRDCAIDPTCVLCMDCFQNSVHKNHRYKMHTSTGGGFCDCGDTEAWKTGPFCVSHEPGRADTTKENLRCPLNEEVIAQARKIFPSVIKYIVEMTIWEEEKELPPELQIREKNERYYCVLFNDEHHSYDHVIYSLQRALDCELAEAQLHTTAIDKEGRRAVKAGAFAACQEAKEDIKSHSENVSQHPLHVEVLHSEVMAHQKFALRLGSWMNKIMSYSSDFRQIFCQACLREEPGSENPCLISRLMLWDAKLYKGARKILHELIFSSFFMEMEYKKFFAMEFVKYYKQLQKEYISDDHDRSISVTALSVQMFTVPTLARHLIEEQNVISVITETLLEVLPEYLDRNNKFNFQGYSQDKLGRVYAVICDLKYILISKPTVWTERLRMQFLEGFRSFLKILTCMQGMEEIRRQVGQHIEVDPDWEAAIAIQMQLKNILLMFQEWCACDKELLLVAYKECHKAVMRCSTSFMSSSKTVVQLCGHTLETKSYKVSEDLVSIHLPLSRTLAGLHVRLSRLGAVSRLHEFVPFEDFQVEVLVEYPLRCLVLVAQVVAEMWRRNGLSLISQVFYYQDVKCREEMYDKDIIMLQIGASLMDPNKFLLLILQRYELADAFNKTISTKDQDLIKQYNTLIEEMLQVLIYIVGERYVPGVGNVTKEEVTMREIIHLLCIEPMPHSAIAKNLPENENNETGLENVINKVATFKKPGVSGHGVYELKDESLKDFNMYFYHYSKTQHSKAEHMQKKRRKQENKDEALPPPPPPEFCPAFSKVVNLLNCDIMMYILRTIFERAIDTDSNLWTEGMLQMAFHLLALGLLEEKQQLQKAPEEEVTFDFYHKASRLGSSAMNAQNIQMLLEKLKGIPQLEGQKDMITWILQMFDTVKRLREKSCLIVATTSGSESIKNDEITHDKEKAERKRKAEAARLHRQKIMAQMSALQKNFIETHKLMYDSTSEMSGKEDSIMEEESTPAVSDYSRIALGPKRGPTVTEKEVLTCILCQEEQEVKIENNAMVLSACVQKSTALTQHRGKPIELSGETVDPLFMDPDLAYGTYTGSCGHVMHAVCWQKYFEAVQLSSQQRIHVDLFDLESGEYLCPLCKSLCNTVIPIIPLQPQRINSENAEALAQLLTLAQWIQTVLARISGYNMKHAKGENPTIPALFDQGMGDSTFEFHSILSFGIQPSIKYSNSIKEMVILFATTIYRIGLKVPPDEMDPRIPMMTWSTCAFTVQAIENLLGDEGKPLFGALQNRQHNGLKALMQFAIAQKITCPQVLIQKHLIRLLSVVLPNLKSEDTPCLLSIDLFHVLVGAVLAFPSLYWDDTVDLQPSSISSSYNHLYLFHLITMAHMLQILLTIDTDIPLAQVQEESEEARSASSFLAEVSQYTSGG

Target ORF information:

RefSeq Version	XM_005638321.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3 component n-recognin 1 (UBR1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638321.2

ORF Insert Sequence:

ATGGCGGATG AGGAGGCCGG GGGTGCGGAG AGGATGGAAG TCAGTACGGA GTTGCCCCAA 
ACCCCTCAGC TACTGGCATC TAGTTGTTCC TGGGTTTGTG GAGAAAACAA GTTGTTGGTC 
TATGCATACC TTAGAAAAGT TGGAGTTAGA AGACTGCAAG TTACAAGTGA CATTATAAGA 
ACACTCTGGA AAAAAAAAAA AAGAACACTC TGGTGGTGGG ATCAGCAAGT AGATTTTTAT 
ACTACTTTCT TGCACCATTT GGCACAGTTG GTGCCAGAAA TTTATTCTGC TGAAATGGAC 
CCAGACTTGG AAAAGCAGGA GGAGAGTATA CAAATGTCCA TATTCACTCC ACTGGAATGG 
TACTTATTTG GAGAAGATCC AGATATTTGC TTAGAGAAAT TGAAACACAG TGGAGCATTT 
CAACTCTGTG GAAAGGTATT CAAAAGTGGA GAGACAACCT ATTCTTGTAG GGACTGTGCA 
ATTGACCCAA CATGTGTACT CTGTATGGAC TGCTTCCAGA ATAGTGTTCA TAAAAACCAT 
CGTTACAAGA TGCATACATC TACTGGAGGG GGATTCTGTG ACTGTGGAGA CACAGAGGCA 
TGGAAAACTG GCCCTTTTTG TGTAAGTCAT GAACCTGGAA GAGCAGATAC TACAAAAGAG 
AATTTACGTT GTCCATTGAA TGAAGAGGTA ATTGCCCAAG CCCGGAAAAT ATTCCCTTCA 
GTGATAAAAT ACATTGTAGA AATGACTATA TGGGAGGAGG AAAAAGAACT GCCTCCTGAA 
CTCCAAATAA GGGAGAAAAA TGAAAGATAC TATTGTGTCC TTTTCAATGA CGAACACCAT 
TCATATGACC ATGTCATATA CAGTCTACAA AGAGCTCTTG ATTGTGAGCT CGCAGAGGCC 
CAGTTGCATA CGACTGCCAT AGACAAAGAG GGCCGTCGGG CTGTCAAAGC AGGTGCTTTT 
GCTGCTTGCC AGGAAGCAAA GGAAGATATA AAGAGCCATT CAGAAAATGT CTCGCAACAT 
CCACTTCATG TAGAAGTATT ACACTCTGAG GTTATGGCTC ATCAGAAATT TGCTTTGCGT 
CTTGGCTCCT GGATGAACAA AATTATGAGC TATTCAAGTG ACTTTAGACA GATCTTTTGC 
CAAGCATGCC TTAGAGAAGA ACCCGGCTCT GAGAATCCCT GTCTCATAAG CAGATTAATG 
CTTTGGGATG CAAAGCTTTA TAAAGGTGCC CGTAAGATCC TTCACGAATT GATCTTCAGC 
AGTTTTTTTA TGGAGATGGA ATACAAAAAA TTCTTTGCTA TGGAATTTGT GAAGTATTAT 
AAACAGCTGC AGAAAGAATA TATCAGTGAT GATCATGACA GAAGTATCTC AGTAACTGCA 
CTCTCAGTTC AAATGTTTAC TGTTCCTACT CTGGCTCGAC ATCTTATTGA AGAGCAGAAT 
GTTATCTCTG TCATTACTGA AACCCTACTA GAAGTTTTGC CTGAATACTT GGACAGGAAC 
AATAAATTCA ACTTTCAAGG TTATAGCCAG GACAAATTGG GAAGAGTATA TGCAGTAATA 
TGTGATCTAA AGTATATCCT AATCAGCAAG CCCACAGTAT GGACAGAAAG ATTAAGAATG 
CAATTCCTTG AAGGTTTTCG ATCTTTTTTG AAGATTCTTA CCTGTATGCA GGGAATGGAA 
GAAATCAGAA GACAGGTTGG GCAACACATA GAAGTGGATC CTGACTGGGA GGCTGCCATT 
GCTATACAGA TGCAATTAAA GAATATCTTA CTCATGTTCC AAGAGTGGTG TGCTTGTGAT 
AAAGAACTCT TACTGGTGGC TTATAAAGAA TGTCACAAAG CTGTGATGAG GTGCAGTACA 
AGTTTTATGT CTAGTAGTAA GACAGTGGTA CAATTGTGTG GTCATACTCT AGAAACTAAG 
TCCTACAAAG TATCTGAAGA TCTTGTAAGC ATACATCTAC CACTCTCTAG GACTCTTGCT 
GGTCTTCATG TGCGTTTAAG CAGGCTGGGA GCTGTTTCAA GACTGCATGA ATTTGTGCCT 
TTTGAGGACT TTCAAGTAGA GGTACTGGTG GAATATCCTT TGCGTTGTCT GGTATTGGTT 
GCCCAGGTTG TTGCTGAGAT GTGGCGAAGA AATGGGCTCT CTCTCATTAG CCAGGTGTTT 
TATTACCAAG ATGTTAAGTG CAGAGAAGAA ATGTATGATA AAGATATCAT TATGCTTCAG 
ATTGGTGCAT CTTTAATGGA TCCTAATAAG TTTTTGTTAC TGATACTTCA GAGGTATGAA 
CTTGCTGATG CTTTTAATAA GACAATATCC ACAAAAGACC AGGATTTGAT TAAACAATAT 
AATACATTAA TAGAAGAAAT GCTTCAAGTC CTCATCTATA TTGTGGGAGA GCGTTATGTA 
CCTGGAGTGG GAAATGTGAC CAAGGAAGAG GTCACAATGA GAGAAATTAT TCACCTGCTT 
TGTATTGAAC CCATGCCACA CAGCGCCATT GCCAAAAATT TACCTGAGAA TGAAAATAAT 
GAAACCGGCC TAGAGAACGT CATTAACAAA GTGGCCACAT TTAAGAAACC AGGTGTGTCA 
GGCCATGGAG TTTATGAACT GAAAGATGAA TCATTGAAAG ACTTCAATAT GTACTTTTAT 
CATTATTCCA AAACACAGCA TAGCAAGGCA GAACATATGC AGAAAAAAAG GAGAAAACAA 
GAAAACAAAG ATGAAGCATT ACCACCACCA CCACCTCCTG AATTCTGCCC TGCTTTCAGC 
AAAGTGGTTA ACCTTCTCAA TTGTGATATC ATGATGTACA TTCTCAGGAC CATATTTGAG 
CGGGCAATTG ACACAGATTC TAACTTGTGG ACTGAAGGGA TGCTTCAGAT GGCATTTCAT 
CTTCTGGCAT TGGGTTTACT AGAAGAAAAG CAACAGCTTC AAAAAGCTCC TGAAGAAGAA 
GTGACATTTG ACTTTTACCA TAAGGCTTCA AGATTGGGAA GTTCAGCCAT GAATGCTCAG 
AATATACAAA TGCTTTTGGA AAAACTCAAA GGAATTCCTC AGTTAGAAGG CCAAAAGGAC 
ATGATAACAT GGATACTCCA GATGTTTGAC ACAGTGAAAC GATTAAGAGA AAAATCTTGT 
TTAATTGTAG CCACTACATC AGGATCAGAA TCTATTAAGA ATGATGAGAT TACTCATGAT 
AAAGAAAAAG CAGAACGGAA AAGAAAAGCT GAAGCTGCTA GATTGCACCG CCAGAAGATC 
ATGGCTCAGA TGTCTGCCTT ACAGAAAAAT TTCATTGAAA CTCACAAACT CATGTATGAT 
AGCACCTCAG AAATGTCTGG GAAAGAAGAC TCCATTATGG AGGAAGAGAG CACCCCAGCA 
GTCAGTGACT ACTCTAGAAT TGCTTTGGGT CCTAAACGGG GTCCGACTGT TACTGAAAAG 
GAGGTGCTGA CATGCATCCT CTGCCAAGAA GAACAAGAGG TGAAAATAGA AAATAATGCC 
ATGGTATTAT CGGCCTGTGT CCAGAAATCC ACCGCCTTAA CCCAGCACAG GGGAAAACCT 
ATAGAACTCT CAGGGGAAAC TGTGGACCCA CTCTTCATGG ATCCAGACTT GGCATATGGA 
ACTTACACAG GAAGCTGTGG TCATGTAATG CATGCGGTGT GCTGGCAGAA GTATTTTGAA 
GCTGTACAGC TGAGCTCTCA GCAGCGCATT CATGTTGACC TTTTTGATTT GGAGAGTGGA 
GAATATCTTT GCCCTCTTTG CAAGTCTCTG TGCAATACTG TGATCCCCAT TATTCCTTTG 
CAACCTCAAA GGATAAACAG TGAGAATGCA GAAGCTCTTG CTCAACTTTT GACCCTGGCA 
CAGTGGATAC AGACTGTTCT GGCCAGAATA TCAGGTTATA ATATGAAACA TGCCAAAGGA 
GAAAACCCAA CAATTCCAGC TTTATTTGAT CAAGGAATGG GCGATTCCAC TTTTGAGTTC 
CATTCTATCC TCAGCTTTGG AATTCAGCCT TCGATAAAAT ATTCTAATAG CATCAAGGAA 
ATGGTCATTC TCTTTGCTAC AACGATTTAT AGAATTGGAC TGAAAGTACC TCCTGATGAA 
ATGGATCCTC GAATCCCCAT GATGACCTGG AGCACGTGTG CTTTCACTGT CCAGGCAATT 
GAAAACCTCT TGGGAGATGA AGGAAAACCT CTGTTTGGAG CACTTCAAAA TAGACAGCAT 
AATGGTCTGA AAGCATTAAT GCAGTTTGCC ATTGCACAGA AGATTACCTG TCCTCAGGTC 
CTGATACAGA AACACCTGAT TCGTCTCCTC TCAGTTGTTC TTCCTAACCT AAAATCAGAA 
GATACACCTT GTCTCCTATC TATAGATCTC TTTCATGTTT TGGTTGGTGC TGTGTTAGCA 
TTCCCATCCT TGTATTGGGA TGACACTGTT GATCTGCAGC CTTCATCAAT TAGTTCTTCC 
TATAACCACC TTTATCTCTT CCATTTGATC ACCATGGCAC ACATGCTTCA GATCCTTCTT 
ACCATAGACA CAGACATTCC CCTTGCTCAG GTTCAAGAAG AGAGTGAAGA GGCTCGTTCT 
GCATCTTCTT TCTTGGCAGA AGTTTCTCAG TATACAAGTG GAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

UBR1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報