NOD2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NOD2
Entrez Gene ID	487286
Full Name	nucleotide binding oligomerization domain containing 2
General protein information	Preferred Names nucleotide binding oligomerization domain containing 2 Names nucleotide-binding oligomerization domain-containing protein 2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005617571.2	XP_005617628.1	nucleotide-binding oligomerization domain-containing protein 2 isoform X1
XM_005617571.1	XP_005617628.1	nucleotide-binding oligomerization domain-containing protein 2 isoform X2
XM_014106638.1	XP_013962113.1	nucleotide-binding oligomerization domain-containing protein 2 isoform X1
NM_001287039.1	NP_001273968.1	nucleotide-binding oligomerization domain-containing protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NOD2	XP_005617627.1
Homo sapiens (human)	NOD2	NP_071445.1
Bos taurus (cattle)	NOD2	NP_001002889.1
Pan troglodytes (chimpanzee)	NOD2	NP_001098710.1
	NOD2	XP_001084287.1
Mus musculus (house mouse)	Nod2	NP_665856.2
Rattus norvegicus (Norway rat)	Nod2	NP_001099642.1
Danio rerio (zebrafish)	nod2	XP_697924.3

Gene Ontology
Bibliography

Related articles in PubMed

Polymorphisms of nucleotide-binding oligomerization domain 2 (NOD2) gene in miniature dachshunds with inflammatory colorectal polyps.
Igarashi H, Ohno K, Uchida E, Fujiwara-Igarashi A, Kanemoto H, Fukushima K, Uchida K, Tsujimoto H
Veterinary immunology and immunopathology164(3-4)160-9(2015 Apr)

Association between nucleotide oligomerisation domain two (Nod2) gene polymorphisms and canine inflammatory bowel disease.
Kathrani A, Lee H, White C, Catchpole B, Murphy A, German A, Werling D, Allenspach K
Veterinary immunology and immunopathology161(1-2)32-41(2014 Sep)

NOD2 mRNA expression and NFkappaB activation in dogs with lymphocytic plasmacytic colitis.
Okanishi H, Hayashi K, Sakamoto Y, Sano T, Maruyama H, Kagawa Y, Watari T
Journal of veterinary internal medicine27(3)439-44(2013 May-Jun)

Toll- and NOD-like receptor mRNA expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinitis.
Mercier E, Peters IR, Day MJ, Clercx C, Peeters D
Veterinary immunology and immunopathology145(3-4)618-24(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that the identified four SNPs (A1532G, T1573C, C1688G, and G1880A in the NOD2 gene) may play a role in the pathogenesis of inflammatory colorectal polyps in miniature dachshunds
Title: Polymorphisms of nucleotide-binding oligomerization domain 2 (NOD2) gene in miniature dachshunds with inflammatory colorectal polyps.

The associations between Nod2 polymorphisms and inflammatory bowel disease in German Shepherd dogs and other breeds were studied.
Title: Association between nucleotide oligomerisation domain two (Nod2) gene polymorphisms and canine inflammatory bowel disease.

These data suggest that NOD2 and NFkappaB play an important role in the pathogenesis of LPC.
Title: NOD2 mRNA expression and NFkappaB activation in dogs with lymphocytic plasmacytic colitis.

This study evaluted the expression of NOD1 and NOD2 proteins in healthy mouse and dog eyes using immunohistochemistry on formalin-fixed, paraffin-embedded globes.
Title: Immunohistochemical detection of NOD1 and NOD2 in the healthy murine and canine eye.

The following NOD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe03325	XM_005617571.2 Latest version!	Canis lupus familiaris nucleotide binding oligomerization domain containing 2 (NOD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe35227	XM_014106638.1 Latest version!	Canis lupus familiaris nucleotide-binding oligomerization domain-containing protein 2 (NOD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe16406	NM_001287039.1 Latest version!	Canis lupus familiaris nucleotide binding oligomerization domain containing 2 (NOD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe03325	XM_005617571.1	Canis lupus familiaris nucleotide-binding oligomerization domain-containing protein 2 (NOD2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NOD2 ( XM_005617571.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617628.1 Canis lupus familiaris nucleotide binding oligomerization domain containing 2 (NOD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe03325
Clone ID Related Accession (Same CDS sequence)	XM_005617571.2 , XM_005617571.1
Accession Version	XM_005617571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	nucleotide-binding oligomerization domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006584.3) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005617571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC TGAGGACCAC GATATCTTCT CAGTCTCGCT TTCTGAGCAC CTATGATGGG 
GCAGAGAATC TTTGCCTGGA GGAAATATAT ACAGAGAATG TTCTGGAGAT CCGAACAGAA 
ATGGGTCTGG CCAGGTCCCC GCAGAAGAGC CCTGCTACCC TAAGCCTGGA GGAACTCTTC 
AGTACCTGTG GCCACCTCAA TGAAGATGCA GACACTGTGC TGGTGGTGGG TGAGGCGGGC 
AGTGGTAAGA GCACATTGCT GCAGCGAATG CACCTGCTGT GGGCTTCAGG GCGCGACTTC 
CAGGAATTTC TCTTTGTCTT CCCATTCAGC TGTCGGCAGC TGCAGTGCGT GGCAAAGCCG 
CTCTCTGTGC AGATGCTGCT CTTTGAACAC TGCTGTTGGC CTGACTTTGG CCAACAGGAA 
GTCTTTCAGT TCCTCCTTGA CCACCCCAAC CGTGTCCTCT TAACCTTCGA TGGCTTTGAT 
GAGTTCAGGT TCAGGTTCTC AGATCATGAG CGTCACTGCT CTCCGACCGA CCCCACATCT 
GTCCAGAATC TGCTGTTCAA CCTTCTGCAG GGCAACTTGC TAAAGAATGC CCGCAAGGTG 
TTGACCAGCC GTCCTGATGC AGTGTCGGCA TTACTCAGGA AGTACCTGCG CTTGGAAATC 
AACCTGAAGG GCTTCTCAGA AGAGGGCATT GAGCTGTACC TGAGGAAGTG CCACCGTGAG 
CCAGGGGTGG CCGACCGCCT CATCCGCCTG CTCAAAACGA CCTCAGCCCT GCATGGTTTG 
TGCCACCTTC CTGTTGTCTC ATGGATGGTG TCCAAATGCC ACCAGGAGCT GTTGTTGCAT 
GGCGGGGGGT CCCCAAAGAC CTCCACGGAC ATGTACCTGC TGATCCTGCA GCATTTTCTG 
CTGCATGCCT CCCCGCCAGA CTCTGTCCCC CACAGTCTGG GATCCCACTT GCTTCGAGGC 
AGGCTCCCCA CCCTCCTGCA CCTGGGCTGG CTGGCTCTCT GGGGTCTGGG CATGTGCTGC 
TACGTGTTCT CCGCCAAGCA GCTCCAGGCA GCACACATTG ATGATGAGGA CATTTCTCTT 
GGCTTCCTGG TGCATGCCAA GACCGTGGGG CCTGGGAGCA CCACCCCCCT GGAATTCCTG 
CACATCACCT TCCAGTGTTT TTTTGCAGCC CTGTACCTTG TGCTCAGCAC TGACGTGTCA 
CCGTCCTTGC TTAGACAACT CTTCATTTGT CATGGGCCGC GCAGCTCGCT GCTGGCCAGG 
CTTCTGCCCA CAACGTGCGT GCCACGCTCA GAGCGCAAGG AGGGCAGCTT GGCAGCTTTG 
CTGCAGGAGG CTGAGCCGCA CAACCTCCAG ATCACGGCAG CCTTCCTGGC GGGGCTGCTG 
TCCCGGGAGC ACCGGGGCCT GCTGGCCGAG TGCCAGGCCA ATGAGGAGGC CCTGCTGCGG 
CTCCAGGGCC GGGCCCAGGG CTGTCTGTCC CGCAGCCTCC ACCAGCACTT CCGCTCCATC 
CCACCAGCTG TGCCAGGGGA GGCCAAGAGC ATGCACGCCA TGCCCGGCTT CCTCTGGCTC 
ATCCGGAGCC TGTACGAGAT GCAGGAGGAG CGGCTGGCCC GGGAGGCGGT GCGTGGGCTA 
ACGGTGGGGC ACCTGAAGCT GACGTTCTGC GGCGTGGGCC CCCCCGAGTG CGCTGCCCTG 
GCCTTTGTGC TGCGGCACCT CCGGCGGCCT GTGGCCCTGC AGCTGGACCA CAACTCTGTT 
GGTGACATTG GCGTGGAGCA GCTGCTGCCG TGCCTCAGTG TCTGCAAGGC TCTTTACTTG 
CGAGATAACA ATATCTCAGA CCGAGGCATC TGTAAGCTCA TCGAGCATGC TCTGCACTGC 
GAGCAGCTGC AGAAGTTAGC GCTGTTCAAC AACAAATTGA CCGATGGCTG TGCACACTCC 
ATGGCGAGGC TCCTTGCATG CAAGCAGAAC TTCCTGGCTT TGAGGCTGGG GAATAACCGT 
ATCACTGCTG CAGGAGCCCA GGCGCTGGCC GAGGGGCTCA GAGCCAACAC CTCCTTGCAG 
TTCTTGGGGT TCTGGGGCAA CAAGGTGGGT GATGAGGGGG CCCAGGCCTT GGCTGAAGCC 
TTGGGTGATC ACCAGAGCTT GAGGTGGCTC AGCCTGGTGG GAAACGACAT TGGCAGTGTC 
GGGGCTCGAG CCTTAGCATT AATGTTGGAA AAGAACGTGG CACTGGAAGA ACTCTGCCTG 
GAGGAGAACC ATCTCCAGGA TGAAGGTGTG TGTTCTCTGG CCAAAGGACT TGAGAGGAAT 
TCAAGTTTGA AAGTTCTGAA GCTGTCCAAC AACTGCATCA CCTACCTTGG GGCAGAAGGC 
CTCCTGCAGG CCCTGGAAAA GAATGATACC ATCCTGGAAG TCTGGCTCCG AGGCAACACT 
TTCTCCTTGG AGGAAATGGA GAGGCTCAGC CAGAAGGACA CCAGACTGTT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617628.1
CDS	158..2614
Translation	MSKLRTTISSQSRFLSTYDGAENLCLEEIYTENVLEIRTEMGLARSPQKSPATLSLEELFSTCGHLNEDADTVLVVGEAGSGKSTLLQRMHLLWASGRDFQEFLFVFPFSCRQLQCVAKPLSVQMLLFEHCCWPDFGQQEVFQFLLDHPNRVLLTFDGFDEFRFRFSDHERHCSPTDPTSVQNLLFNLLQGNLLKNARKVLTSRPDAVSALLRKYLRLEINLKGFSEEGIELYLRKCHREPGVADRLIRLLKTTSALHGLCHLPVVSWMVSKCHQELLLHGGGSPKTSTDMYLLILQHFLLHASPPDSVPHSLGSHLLRGRLPTLLHLGWLALWGLGMCCYVFSAKQLQAAHIDDEDISLGFLVHAKTVGPGSTTPLEFLHITFQCFFAALYLVLSTDVSPSLLRQLFICHGPRSSLLARLLPTTCVPRSERKEGSLAALLQEAEPHNLQITAAFLAGLLSREHRGLLAECQANEEALLRLQGRAQGCLSRSLHQHFRSIPPAVPGEAKSMHAMPGFLWLIRSLYEMQEERLAREAVRGLTVGHLKLTFCGVGPPECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPCLSVCKALYLRDNNISDRGICKLIEHALHCEQLQKLALFNNKLTDGCAHSMARLLACKQNFLALRLGNNRITAAGAQALAEGLRANTSLQFLGFWGNKVGDEGAQALAEALGDHQSLRWLSLVGNDIGSVGARALALMLEKNVALEELCLEENHLQDEGVCSLAKGLERNSSLKVLKLSNNCITYLGAEGLLQALEKNDTILEVWLRGNTFSLEEMERLSQKDTRLLL

Target ORF information:

RefSeq Version	XM_005617571.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nucleotide binding oligomerization domain containing 2 (NOD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617571.2

ORF Insert Sequence:

ATGTCCAAGC TGAGGACCAC GATATCTTCT CAGTCTCGCT TTCTGAGCAC CTATGATGGG 
GCAGAGAATC TTTGCCTGGA GGAAATATAT ACAGAGAATG TTCTGGAGAT CCGAACAGAA 
ATGGGTCTGG CCAGGTCCCC GCAGAAGAGC CCTGCTACCC TAAGCCTGGA GGAACTCTTC 
AGTACCTGTG GCCACCTCAA TGAAGATGCA GACACTGTGC TGGTGGTGGG TGAGGCGGGC 
AGTGGTAAGA GCACATTGCT GCAGCGAATG CACCTGCTGT GGGCTTCAGG GCGCGACTTC 
CAGGAATTTC TCTTTGTCTT CCCATTCAGC TGTCGGCAGC TGCAGTGCGT GGCAAAGCCG 
CTCTCTGTGC AGATGCTGCT CTTTGAACAC TGCTGTTGGC CTGACTTTGG CCAACAGGAA 
GTCTTTCAGT TCCTCCTTGA CCACCCCAAC CGTGTCCTCT TAACCTTCGA TGGCTTTGAT 
GAGTTCAGGT TCAGGTTCTC AGATCATGAG CGTCACTGCT CTCCGACCGA CCCCACATCT 
GTCCAGAATC TGCTGTTCAA CCTTCTGCAG GGCAACTTGC TAAAGAATGC CCGCAAGGTG 
TTGACCAGCC GTCCTGATGC AGTGTCGGCA TTACTCAGGA AGTACCTGCG CTTGGAAATC 
AACCTGAAGG GCTTCTCAGA AGAGGGCATT GAGCTGTACC TGAGGAAGTG CCACCGTGAG 
CCAGGGGTGG CCGACCGCCT CATCCGCCTG CTCAAAACGA CCTCAGCCCT GCATGGTTTG 
TGCCACCTTC CTGTTGTCTC ATGGATGGTG TCCAAATGCC ACCAGGAGCT GTTGTTGCAT 
GGCGGGGGGT CCCCAAAGAC CTCCACGGAC ATGTACCTGC TGATCCTGCA GCATTTTCTG 
CTGCATGCCT CCCCGCCAGA CTCTGTCCCC CACAGTCTGG GATCCCACTT GCTTCGAGGC 
AGGCTCCCCA CCCTCCTGCA CCTGGGCTGG CTGGCTCTCT GGGGTCTGGG CATGTGCTGC 
TACGTGTTCT CCGCCAAGCA GCTCCAGGCA GCACACATTG ATGATGAGGA CATTTCTCTT 
GGCTTCCTGG TGCATGCCAA GACCGTGGGG CCTGGGAGCA CCACCCCCCT GGAATTCCTG 
CACATCACCT TCCAGTGTTT TTTTGCAGCC CTGTACCTTG TGCTCAGCAC TGACGTGTCA 
CCGTCCTTGC TTAGACAACT CTTCATTTGT CATGGGCCGC GCAGCTCGCT GCTGGCCAGG 
CTTCTGCCCA CAACGTGCGT GCCACGCTCA GAGCGCAAGG AGGGCAGCTT GGCAGCTTTG 
CTGCAGGAGG CTGAGCCGCA CAACCTCCAG ATCACGGCAG CCTTCCTGGC GGGGCTGCTG 
TCCCGGGAGC ACCGGGGCCT GCTGGCCGAG TGCCAGGCCA ATGAGGAGGC CCTGCTGCGG 
CTCCAGGGCC GGGCCCAGGG CTGTCTGTCC CGCAGCCTCC ACCAGCACTT CCGCTCCATC 
CCACCAGCTG TGCCAGGGGA GGCCAAGAGC ATGCACGCCA TGCCCGGCTT CCTCTGGCTC 
ATCCGGAGCC TGTACGAGAT GCAGGAGGAG CGGCTGGCCC GGGAGGCGGT GCGTGGGCTA 
ACGGTGGGGC ACCTGAAGCT GACGTTCTGC GGCGTGGGCC CCCCCGAGTG CGCTGCCCTG 
GCCTTTGTGC TGCGGCACCT CCGGCGGCCT GTGGCCCTGC AGCTGGACCA CAACTCTGTT 
GGTGACATTG GCGTGGAGCA GCTGCTGCCG TGCCTCAGTG TCTGCAAGGC TCTTTACTTG 
CGAGATAACA ATATCTCAGA CCGAGGCATC TGTAAGCTCA TCGAGCATGC TCTGCACTGC 
GAGCAGCTGC AGAAGTTAGC GCTGTTCAAC AACAAATTGA CCGATGGCTG TGCACACTCC 
ATGGCGAGGC TCCTTGCATG CAAGCAGAAC TTCCTGGCTT TGAGGCTGGG GAATAACCGT 
ATCACTGCTG CAGGAGCCCA GGCGCTGGCC GAGGGGCTCA GAGCCAACAC CTCCTTGCAG 
TTCTTGGGGT TCTGGGGCAA CAAGGTGGGT GATGAGGGGG CCCAGGCCTT GGCTGAAGCC 
TTGGGTGATC ACCAGAGCTT GAGGTGGCTC AGCCTGGTGG GAAACGACAT TGGCAGTGTC 
GGGGCTCGAG CCTTAGCATT AATGTTGGAA AAGAACGTGG CACTGGAAGA ACTCTGCCTG 
GAGGAGAACC ATCTCCAGGA TGAAGGTGTG TGTTCTCTGG CCAAAGGACT TGAGAGGAAT 
TCAAGTTTGA AAGTTCTGAA GCTGTCCAAC AACTGCATCA CCTACCTTGG GGCAGAAGGC 
CTCCTGCAGG CCCTGGAAAA GAATGATACC ATCCTGGAAG TCTGGCTCCG AGGCAACACT 
TTCTCCTTGG AGGAAATGGA GAGGCTCAGC CAGAAGGACA CCAGACTGTT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOD2 ( XM_005617571.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005617628.1 Canis lupus familiaris nucleotide-binding oligomerization domain-containing protein 2 (NOD2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe03325
Clone ID Related Accession (Same CDS sequence)	XM_005617571.2 , XM_005617571.1
Accession Version	XM_005617571.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nucleotide-binding oligomerization domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAAGC TGAGGACCAC GATATCTTCT CAGTCTCGCT TTCTGAGCAC CTATGATGGG 
GCAGAGAATC TTTGCCTGGA GGAAATATAT ACAGAGAATG TTCTGGAGAT CCGAACAGAA 
ATGGGTCTGG CCAGGTCCCC GCAGAAGAGC CCTGCTACCC TAAGCCTGGA GGAACTCTTC 
AGTACCTGTG GCCACCTCAA TGAAGATGCA GACACTGTGC TGGTGGTGGG TGAGGCGGGC 
AGTGGTAAGA GCACATTGCT GCAGCGAATG CACCTGCTGT GGGCTTCAGG GCGCGACTTC 
CAGGAATTTC TCTTTGTCTT CCCATTCAGC TGTCGGCAGC TGCAGTGCGT GGCAAAGCCG 
CTCTCTGTGC AGATGCTGCT CTTTGAACAC TGCTGTTGGC CTGACTTTGG CCAACAGGAA 
GTCTTTCAGT TCCTCCTTGA CCACCCCAAC CGTGTCCTCT TAACCTTCGA TGGCTTTGAT 
GAGTTCAGGT TCAGGTTCTC AGATCATGAG CGTCACTGCT CTCCGACCGA CCCCACATCT 
GTCCAGAATC TGCTGTTCAA CCTTCTGCAG GGCAACTTGC TAAAGAATGC CCGCAAGGTG 
TTGACCAGCC GTCCTGATGC AGTGTCGGCA TTACTCAGGA AGTACCTGCG CTTGGAAATC 
AACCTGAAGG GCTTCTCAGA AGAGGGCATT GAGCTGTACC TGAGGAAGTG CCACCGTGAG 
CCAGGGGTGG CCGACCGCCT CATCCGCCTG CTCAAAACGA CCTCAGCCCT GCATGGTTTG 
TGCCACCTTC CTGTTGTCTC ATGGATGGTG TCCAAATGCC ACCAGGAGCT GTTGTTGCAT 
GGCGGGGGGT CCCCAAAGAC CTCCACGGAC ATGTACCTGC TGATCCTGCA GCATTTTCTG 
CTGCATGCCT CCCCGCCAGA CTCTGTCCCC CACAGTCTGG GATCCCACTT GCTTCGAGGC 
AGGCTCCCCA CCCTCCTGCA CCTGGGCTGG CTGGCTCTCT GGGGTCTGGG CATGTGCTGC 
TACGTGTTCT CCGCCAAGCA GCTCCAGGCA GCACACATTG ATGATGAGGA CATTTCTCTT 
GGCTTCCTGG TGCATGCCAA GACCGTGGGG CCTGGGAGCA CCACCCCCCT GGAATTCCTG 
CACATCACCT TCCAGTGTTT TTTTGCAGCC CTGTACCTTG TGCTCAGCAC TGACGTGTCA 
CCGTCCTTGC TTAGACAACT CTTCATTTGT CATGGGCCGC GCAGCTCGCT GCTGGCCAGG 
CTTCTGCCCA CAACGTGCGT GCCACGCTCA GAGCGCAAGG AGGGCAGCTT GGCAGCTTTG 
CTGCAGGAGG CTGAGCCGCA CAACCTCCAG ATCACGGCAG CCTTCCTGGC GGGGCTGCTG 
TCCCGGGAGC ACCGGGGCCT GCTGGCCGAG TGCCAGGCCA ATGAGGAGGC CCTGCTGCGG 
CTCCAGGGCC GGGCCCAGGG CTGTCTGTCC CGCAGCCTCC ACCAGCACTT CCGCTCCATC 
CCACCAGCTG TGCCAGGGGA GGCCAAGAGC ATGCACGCCA TGCCCGGCTT CCTCTGGCTC 
ATCCGGAGCC TGTACGAGAT GCAGGAGGAG CGGCTGGCCC GGGAGGCGGT GCGTGGGCTA 
ACGGTGGGGC ACCTGAAGCT GACGTTCTGC GGCGTGGGCC CCCCCGAGTG CGCTGCCCTG 
GCCTTTGTGC TGCGGCACCT CCGGCGGCCT GTGGCCCTGC AGCTGGACCA CAACTCTGTT 
GGTGACATTG GCGTGGAGCA GCTGCTGCCG TGCCTCAGTG TCTGCAAGGC TCTTTACTTG 
CGAGATAACA ATATCTCAGA CCGAGGCATC TGTAAGCTCA TCGAGCATGC TCTGCACTGC 
GAGCAGCTGC AGAAGTTAGC GCTGTTCAAC AACAAATTGA CCGATGGCTG TGCACACTCC 
ATGGCGAGGC TCCTTGCATG CAAGCAGAAC TTCCTGGCTT TGAGGCTGGG GAATAACCGT 
ATCACTGCTG CAGGAGCCCA GGCGCTGGCC GAGGGGCTCA GAGCCAACAC CTCCTTGCAG 
TTCTTGGGGT TCTGGGGCAA CAAGGTGGGT GATGAGGGGG CCCAGGCCTT GGCTGAAGCC 
TTGGGTGATC ACCAGAGCTT GAGGTGGCTC AGCCTGGTGG GAAACGACAT TGGCAGTGTC 
GGGGCTCGAG CCTTAGCATT AATGTTGGAA AAGAACGTGG CACTGGAAGA ACTCTGCCTG 
GAGGAGAACC ATCTCCAGGA TGAAGGTGTG TGTTCTCTGG CCAAAGGACT TGAGAGGAAT 
TCAAGTTTGA AAGTTCTGAA GCTGTCCAAC AACTGCATCA CCTACCTTGG GGCAGAAGGC 
CTCCTGCAGG CCCTGGAAAA GAATGATACC ATCCTGGAAG TCTGGCTCCG AGGCAACACT 
TTCTCCTTGG AGGAAATGGA GAGGCTCAGC CAGAAGGACA CCAGACTGTT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005617628.1
CDS	106..2562
Translation	MSKLRTTISSQSRFLSTYDGAENLCLEEIYTENVLEIRTEMGLARSPQKSPATLSLEELFSTCGHLNEDADTVLVVGEAGSGKSTLLQRMHLLWASGRDFQEFLFVFPFSCRQLQCVAKPLSVQMLLFEHCCWPDFGQQEVFQFLLDHPNRVLLTFDGFDEFRFRFSDHERHCSPTDPTSVQNLLFNLLQGNLLKNARKVLTSRPDAVSALLRKYLRLEINLKGFSEEGIELYLRKCHREPGVADRLIRLLKTTSALHGLCHLPVVSWMVSKCHQELLLHGGGSPKTSTDMYLLILQHFLLHASPPDSVPHSLGSHLLRGRLPTLLHLGWLALWGLGMCCYVFSAKQLQAAHIDDEDISLGFLVHAKTVGPGSTTPLEFLHITFQCFFAALYLVLSTDVSPSLLRQLFICHGPRSSLLARLLPTTCVPRSERKEGSLAALLQEAEPHNLQITAAFLAGLLSREHRGLLAECQANEEALLRLQGRAQGCLSRSLHQHFRSIPPAVPGEAKSMHAMPGFLWLIRSLYEMQEERLAREAVRGLTVGHLKLTFCGVGPPECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPCLSVCKALYLRDNNISDRGICKLIEHALHCEQLQKLALFNNKLTDGCAHSMARLLACKQNFLALRLGNNRITAAGAQALAEGLRANTSLQFLGFWGNKVGDEGAQALAEALGDHQSLRWLSLVGNDIGSVGARALALMLEKNVALEELCLEENHLQDEGVCSLAKGLERNSSLKVLKLSNNCITYLGAEGLLQALEKNDTILEVWLRGNTFSLEEMERLSQKDTRLLL

Target ORF information:

RefSeq Version	XM_005617571.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nucleotide-binding oligomerization domain-containing protein 2 (NOD2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005617571.1

ORF Insert Sequence:

ATGTCCAAGC TGAGGACCAC GATATCTTCT CAGTCTCGCT TTCTGAGCAC CTATGATGGG 
GCAGAGAATC TTTGCCTGGA GGAAATATAT ACAGAGAATG TTCTGGAGAT CCGAACAGAA 
ATGGGTCTGG CCAGGTCCCC GCAGAAGAGC CCTGCTACCC TAAGCCTGGA GGAACTCTTC 
AGTACCTGTG GCCACCTCAA TGAAGATGCA GACACTGTGC TGGTGGTGGG TGAGGCGGGC 
AGTGGTAAGA GCACATTGCT GCAGCGAATG CACCTGCTGT GGGCTTCAGG GCGCGACTTC 
CAGGAATTTC TCTTTGTCTT CCCATTCAGC TGTCGGCAGC TGCAGTGCGT GGCAAAGCCG 
CTCTCTGTGC AGATGCTGCT CTTTGAACAC TGCTGTTGGC CTGACTTTGG CCAACAGGAA 
GTCTTTCAGT TCCTCCTTGA CCACCCCAAC CGTGTCCTCT TAACCTTCGA TGGCTTTGAT 
GAGTTCAGGT TCAGGTTCTC AGATCATGAG CGTCACTGCT CTCCGACCGA CCCCACATCT 
GTCCAGAATC TGCTGTTCAA CCTTCTGCAG GGCAACTTGC TAAAGAATGC CCGCAAGGTG 
TTGACCAGCC GTCCTGATGC AGTGTCGGCA TTACTCAGGA AGTACCTGCG CTTGGAAATC 
AACCTGAAGG GCTTCTCAGA AGAGGGCATT GAGCTGTACC TGAGGAAGTG CCACCGTGAG 
CCAGGGGTGG CCGACCGCCT CATCCGCCTG CTCAAAACGA CCTCAGCCCT GCATGGTTTG 
TGCCACCTTC CTGTTGTCTC ATGGATGGTG TCCAAATGCC ACCAGGAGCT GTTGTTGCAT 
GGCGGGGGGT CCCCAAAGAC CTCCACGGAC ATGTACCTGC TGATCCTGCA GCATTTTCTG 
CTGCATGCCT CCCCGCCAGA CTCTGTCCCC CACAGTCTGG GATCCCACTT GCTTCGAGGC 
AGGCTCCCCA CCCTCCTGCA CCTGGGCTGG CTGGCTCTCT GGGGTCTGGG CATGTGCTGC 
TACGTGTTCT CCGCCAAGCA GCTCCAGGCA GCACACATTG ATGATGAGGA CATTTCTCTT 
GGCTTCCTGG TGCATGCCAA GACCGTGGGG CCTGGGAGCA CCACCCCCCT GGAATTCCTG 
CACATCACCT TCCAGTGTTT TTTTGCAGCC CTGTACCTTG TGCTCAGCAC TGACGTGTCA 
CCGTCCTTGC TTAGACAACT CTTCATTTGT CATGGGCCGC GCAGCTCGCT GCTGGCCAGG 
CTTCTGCCCA CAACGTGCGT GCCACGCTCA GAGCGCAAGG AGGGCAGCTT GGCAGCTTTG 
CTGCAGGAGG CTGAGCCGCA CAACCTCCAG ATCACGGCAG CCTTCCTGGC GGGGCTGCTG 
TCCCGGGAGC ACCGGGGCCT GCTGGCCGAG TGCCAGGCCA ATGAGGAGGC CCTGCTGCGG 
CTCCAGGGCC GGGCCCAGGG CTGTCTGTCC CGCAGCCTCC ACCAGCACTT CCGCTCCATC 
CCACCAGCTG TGCCAGGGGA GGCCAAGAGC ATGCACGCCA TGCCCGGCTT CCTCTGGCTC 
ATCCGGAGCC TGTACGAGAT GCAGGAGGAG CGGCTGGCCC GGGAGGCGGT GCGTGGGCTA 
ACGGTGGGGC ACCTGAAGCT GACGTTCTGC GGCGTGGGCC CCCCCGAGTG CGCTGCCCTG 
GCCTTTGTGC TGCGGCACCT CCGGCGGCCT GTGGCCCTGC AGCTGGACCA CAACTCTGTT 
GGTGACATTG GCGTGGAGCA GCTGCTGCCG TGCCTCAGTG TCTGCAAGGC TCTTTACTTG 
CGAGATAACA ATATCTCAGA CCGAGGCATC TGTAAGCTCA TCGAGCATGC TCTGCACTGC 
GAGCAGCTGC AGAAGTTAGC GCTGTTCAAC AACAAATTGA CCGATGGCTG TGCACACTCC 
ATGGCGAGGC TCCTTGCATG CAAGCAGAAC TTCCTGGCTT TGAGGCTGGG GAATAACCGT 
ATCACTGCTG CAGGAGCCCA GGCGCTGGCC GAGGGGCTCA GAGCCAACAC CTCCTTGCAG 
TTCTTGGGGT TCTGGGGCAA CAAGGTGGGT GATGAGGGGG CCCAGGCCTT GGCTGAAGCC 
TTGGGTGATC ACCAGAGCTT GAGGTGGCTC AGCCTGGTGG GAAACGACAT TGGCAGTGTC 
GGGGCTCGAG CCTTAGCATT AATGTTGGAA AAGAACGTGG CACTGGAAGA ACTCTGCCTG 
GAGGAGAACC ATCTCCAGGA TGAAGGTGTG TGTTCTCTGG CCAAAGGACT TGAGAGGAAT 
TCAAGTTTGA AAGTTCTGAA GCTGTCCAAC AACTGCATCA CCTACCTTGG GGCAGAAGGC 
CTCCTGCAGG CCCTGGAAAA GAATGATACC ATCCTGGAAG TCTGGCTCCG AGGCAACACT 
TTCTCCTTGG AGGAAATGGA GAGGCTCAGC CAGAAGGACA CCAGACTGTT GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOD2 ( XM_014106638.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962113.1 Canis lupus familiaris nucleotide-binding oligomerization domain-containing protein 2 (NOD2), transcript variant X1, mRNA.

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CloneID	OCe35227
Clone ID Related Accession (Same CDS sequence)	XM_014106638.1
Accession Version	XM_014106638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	nucleotide-binding oligomerization domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726050.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTCTG AAGATGCCGC ATGTAAGAAG TACATGTCCA AGCTGAGGAC CACGATATCT 
TCTCAGTCTC GCTTTCTGAG CACCTATGAT GGGGCAGAGA ATCTTTGCCT GGAGGAAATA 
TATACAGAGA ATGTTCTGGA GATCCGAACA GAAATGGGTC TGGCCAGGTC CCCGCAGAAG 
AGCCCTGCTA CCCTAAGCCT GGAGGAACTC TTCAGTACCT GTGGCCACCT CAATGAAGAT 
GCAGACACTG TGCTGGTGGT GGGTGAGGCG GGCAGTGGTA AGAGCACATT GCTGCAGCGA 
ATGCACCTGC TGTGGGCTTC AGGGCGCGAC TTCCAGGAAT TTCTCTTTGT CTTCCCATTC 
AGCTGTCGGC AGCTGCAGTG CGTGGCAAAG CCGCTCTCTG TGCAGATGCT GCTCTTTGAA 
CACTGCTGTT GGCCTGACTT TGGCCAACAG GAAGTCTTTC AGTTCCTCCT TGACCACCCC 
AACCGTGTCC TCTTAACCTT CGATGGCTTT GATGAGTTCA GGTTCAGGTT CTCAGATCAT 
GAGCGTCACT GCTCTCCGAC CGACCCCACA TCTGTCCAGA ATCTGCTGTT CAACCTTCTG 
CAGGGCAACT TGCTAAAGAA TGCCCGCAAG GTGTTGACCA GCCGTCCTGA TGCAGTGTCG 
GCATTACTCA GGAAGTACCT GCGCTTGGAA ATCAACCTGA AGGGCTTCTC AGAAGAGGGC 
ATTGAGCTGT ACCTGAGGAA GTGCCACCGT GAGCCAGGGG TGGCCGACCG CCTCATCCGC 
CTGCTCAAAA CGACCTCAGC CCTGCATGGT TTGTGCCACC TTCCTGTTGT CTCATGGATG 
GTGTCCAAAT GCCACCAGGA GCTGTTGTTG CATGGCGGGG GGTCCCCAAA GACCTCCACG 
GACATGTACC TGCTGATCCT GCAGCATTTT CTGCTGCATG CCTCCCCGCC AGACTCTGTC 
CCCCACAGTC TGGGATCCCA CTTGCTTCGA GGCAGGCTCC CCACCCTCCT GCACCTGGGC 
TGGCTGGCTC TCTGGGGTCT GGGCATGTGC TGCTACGTGT TCTCCGCCAA GCAGCTCCAG 
GCAGCACACA TTGATGATGA GGACATTTCT CTTGGCTTCC TGGTGCATGC CAAGACCGTG 
GGGCCTGGGA GCACCACCCC CCTGGAATTC CTGCACATCA CCTTCCAGTG TTTTTTTGCA 
GCCCTGTACC TTGTGCTCAG CACTGACGTG TCACCGTCCT TGCTTAGACA ACTCTTCATT 
TGTCATGGGC CGCGCAGCTC GCTGCTGGCC AGGCTTCTGC CCACAACGTG CGTGCCACGC 
TCAGAGCGCA AGGAGGGCAG CTTGGCAGCT TTGCTGCAGG AGGCTGAGCC GCACAACCTC 
CAGATCACGG CAGCCTTCCT GGCGGGGCTG CTGTCCCGGG AGCACCGGGG CCTGCTGGCC 
GAGTGCCAGG CCAATGAGGA GGCCCTGCTG CGGCTCCAGG GCCGGGCCCA GGGCTGTCTG 
TCCCGCAGCC TCCACCAGCA CTTCCGCTCC ATCCCACCAG CTGTGCCAGG GGAGGCCAAG 
AGCATGCACG CCATGCCCGG CTTCCTCTGG CTCATCCGGA GCCTGTACGA GATGCAGGAG 
GAGCGGCTGG CCCGGGAGGC GGTGCGTGGG CTAACGGTGG GGCACCTGAA GCTGACGTTC 
TGCGGCGTGG GCCCCCCCGA GTGCGCTGCC CTGGCCTTTG TGCTGCGGCA CCTCCGGCGG 
CCTGTGGCCC TGCAGCTGGA CCACAACTCT GTTGGTGACA TTGGCGTGGA GCAGCTGCTG 
CCGTGCCTCA GTGTCTGCAA GGCTCTTTAC TTGCGAGATA ACAATATCTC AGACCGAGGC 
ATCTGTAAGC TCATCGAGCA TGCTCTGCAC TGCGAGCAGC TGCAGAAGTT AGCGCTGTTC 
AACAACAAAT TGACCGATGG CTGTGCACAC TCCATGGCGA GGCTCCTTGC ATGCAAGCAG 
AACTTCCTGG CTTTGAGGCT GGGGAATAAC CGTATCACTG CTGCAGGAGC CCAGGCGCTG 
GCCGAGGGGC TCAGAGCCAA CACCTCCTTG CAGTTCTTGG GGTTCTGGGG CAACAAGGTG 
GGTGATGAGG GGGCCCAGGC CTTGGCTGAA GCCTTGGGTG ATCACCAGAG CTTGAGGTGG 
CTCAGCCTGG TGGGAAACGA CATTGGCAGT GTCGGGGCTC GAGCCTTAGC ATTAATGTTG 
GAAAAGAACG TGGCACTGGA AGAACTCTGC CTGGAGGAGA ACCATCTCCA GGATGAAGGT 
GTGTGTTCTC TGGCCAAAGG ACTTGAGAGG AATTCAAGTT TGAAAGTTCT GAAGCTGTCC 
AACAACTGCA TCACCTACCT TGGGGCAGAA GGCCTCCTGC AGGCCCTGGA AAAGAATGAT 
ACCATCCTGG AAGTCTGGCT CCGAGGCAAC ACTTTCTCCT TGGAGGAAAT GGAGAGGCTC 
AGCCAGAAGG ACACCAGACT GTTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962113.1
CDS	238..2727
Translation	MPSEDAACKKYMSKLRTTISSQSRFLSTYDGAENLCLEEIYTENVLEIRTEMGLARSPQKSPATLSLEELFSTCGHLNEDADTVLVVGEAGSGKSTLLQRMHLLWASGRDFQEFLFVFPFSCRQLQCVAKPLSVQMLLFEHCCWPDFGQQEVFQFLLDHPNRVLLTFDGFDEFRFRFSDHERHCSPTDPTSVQNLLFNLLQGNLLKNARKVLTSRPDAVSALLRKYLRLEINLKGFSEEGIELYLRKCHREPGVADRLIRLLKTTSALHGLCHLPVVSWMVSKCHQELLLHGGGSPKTSTDMYLLILQHFLLHASPPDSVPHSLGSHLLRGRLPTLLHLGWLALWGLGMCCYVFSAKQLQAAHIDDEDISLGFLVHAKTVGPGSTTPLEFLHITFQCFFAALYLVLSTDVSPSLLRQLFICHGPRSSLLARLLPTTCVPRSERKEGSLAALLQEAEPHNLQITAAFLAGLLSREHRGLLAECQANEEALLRLQGRAQGCLSRSLHQHFRSIPPAVPGEAKSMHAMPGFLWLIRSLYEMQEERLAREAVRGLTVGHLKLTFCGVGPPECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPCLSVCKALYLRDNNISDRGICKLIEHALHCEQLQKLALFNNKLTDGCAHSMARLLACKQNFLALRLGNNRITAAGAQALAEGLRANTSLQFLGFWGNKVGDEGAQALAEALGDHQSLRWLSLVGNDIGSVGARALALMLEKNVALEELCLEENHLQDEGVCSLAKGLERNSSLKVLKLSNNCITYLGAEGLLQALEKNDTILEVWLRGNTFSLEEMERLSQKDTRLLL

Target ORF information:

RefSeq Version	XM_014106638.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris nucleotide-binding oligomerization domain-containing protein 2 (NOD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106638.1

ORF Insert Sequence:

ATGCCTTCTG AAGATGCCGC ATGTAAGAAG TACATGTCCA AGCTGAGGAC CACGATATCT 
TCTCAGTCTC GCTTTCTGAG CACCTATGAT GGGGCAGAGA ATCTTTGCCT GGAGGAAATA 
TATACAGAGA ATGTTCTGGA GATCCGAACA GAAATGGGTC TGGCCAGGTC CCCGCAGAAG 
AGCCCTGCTA CCCTAAGCCT GGAGGAACTC TTCAGTACCT GTGGCCACCT CAATGAAGAT 
GCAGACACTG TGCTGGTGGT GGGTGAGGCG GGCAGTGGTA AGAGCACATT GCTGCAGCGA 
ATGCACCTGC TGTGGGCTTC AGGGCGCGAC TTCCAGGAAT TTCTCTTTGT CTTCCCATTC 
AGCTGTCGGC AGCTGCAGTG CGTGGCAAAG CCGCTCTCTG TGCAGATGCT GCTCTTTGAA 
CACTGCTGTT GGCCTGACTT TGGCCAACAG GAAGTCTTTC AGTTCCTCCT TGACCACCCC 
AACCGTGTCC TCTTAACCTT CGATGGCTTT GATGAGTTCA GGTTCAGGTT CTCAGATCAT 
GAGCGTCACT GCTCTCCGAC CGACCCCACA TCTGTCCAGA ATCTGCTGTT CAACCTTCTG 
CAGGGCAACT TGCTAAAGAA TGCCCGCAAG GTGTTGACCA GCCGTCCTGA TGCAGTGTCG 
GCATTACTCA GGAAGTACCT GCGCTTGGAA ATCAACCTGA AGGGCTTCTC AGAAGAGGGC 
ATTGAGCTGT ACCTGAGGAA GTGCCACCGT GAGCCAGGGG TGGCCGACCG CCTCATCCGC 
CTGCTCAAAA CGACCTCAGC CCTGCATGGT TTGTGCCACC TTCCTGTTGT CTCATGGATG 
GTGTCCAAAT GCCACCAGGA GCTGTTGTTG CATGGCGGGG GGTCCCCAAA GACCTCCACG 
GACATGTACC TGCTGATCCT GCAGCATTTT CTGCTGCATG CCTCCCCGCC AGACTCTGTC 
CCCCACAGTC TGGGATCCCA CTTGCTTCGA GGCAGGCTCC CCACCCTCCT GCACCTGGGC 
TGGCTGGCTC TCTGGGGTCT GGGCATGTGC TGCTACGTGT TCTCCGCCAA GCAGCTCCAG 
GCAGCACACA TTGATGATGA GGACATTTCT CTTGGCTTCC TGGTGCATGC CAAGACCGTG 
GGGCCTGGGA GCACCACCCC CCTGGAATTC CTGCACATCA CCTTCCAGTG TTTTTTTGCA 
GCCCTGTACC TTGTGCTCAG CACTGACGTG TCACCGTCCT TGCTTAGACA ACTCTTCATT 
TGTCATGGGC CGCGCAGCTC GCTGCTGGCC AGGCTTCTGC CCACAACGTG CGTGCCACGC 
TCAGAGCGCA AGGAGGGCAG CTTGGCAGCT TTGCTGCAGG AGGCTGAGCC GCACAACCTC 
CAGATCACGG CAGCCTTCCT GGCGGGGCTG CTGTCCCGGG AGCACCGGGG CCTGCTGGCC 
GAGTGCCAGG CCAATGAGGA GGCCCTGCTG CGGCTCCAGG GCCGGGCCCA GGGCTGTCTG 
TCCCGCAGCC TCCACCAGCA CTTCCGCTCC ATCCCACCAG CTGTGCCAGG GGAGGCCAAG 
AGCATGCACG CCATGCCCGG CTTCCTCTGG CTCATCCGGA GCCTGTACGA GATGCAGGAG 
GAGCGGCTGG CCCGGGAGGC GGTGCGTGGG CTAACGGTGG GGCACCTGAA GCTGACGTTC 
TGCGGCGTGG GCCCCCCCGA GTGCGCTGCC CTGGCCTTTG TGCTGCGGCA CCTCCGGCGG 
CCTGTGGCCC TGCAGCTGGA CCACAACTCT GTTGGTGACA TTGGCGTGGA GCAGCTGCTG 
CCGTGCCTCA GTGTCTGCAA GGCTCTTTAC TTGCGAGATA ACAATATCTC AGACCGAGGC 
ATCTGTAAGC TCATCGAGCA TGCTCTGCAC TGCGAGCAGC TGCAGAAGTT AGCGCTGTTC 
AACAACAAAT TGACCGATGG CTGTGCACAC TCCATGGCGA GGCTCCTTGC ATGCAAGCAG 
AACTTCCTGG CTTTGAGGCT GGGGAATAAC CGTATCACTG CTGCAGGAGC CCAGGCGCTG 
GCCGAGGGGC TCAGAGCCAA CACCTCCTTG CAGTTCTTGG GGTTCTGGGG CAACAAGGTG 
GGTGATGAGG GGGCCCAGGC CTTGGCTGAA GCCTTGGGTG ATCACCAGAG CTTGAGGTGG 
CTCAGCCTGG TGGGAAACGA CATTGGCAGT GTCGGGGCTC GAGCCTTAGC ATTAATGTTG 
GAAAAGAACG TGGCACTGGA AGAACTCTGC CTGGAGGAGA ACCATCTCCA GGATGAAGGT 
GTGTGTTCTC TGGCCAAAGG ACTTGAGAGG AATTCAAGTT TGAAAGTTCT GAAGCTGTCC 
AACAACTGCA TCACCTACCT TGGGGCAGAA GGCCTCCTGC AGGCCCTGGA AAAGAATGAT 
ACCATCCTGG AAGTCTGGCT CCGAGGCAAC ACTTTCTCCT TGGAGGAAAT GGAGAGGCTC 
AGCCAGAAGG ACACCAGACT GTTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NOD2 ( NM_001287039.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001273968.1 Canis lupus familiaris nucleotide binding oligomerization domain containing 2 (NOD2), mRNA.

Price & Availability↑

CloneID	OCe16406
Clone ID Related Accession (Same CDS sequence)	NM_001287039.1
Accession Version	NM_001287039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Canis lupus familiaris(dog)
Product	nucleotide-binding oligomerization domain-containing protein 2
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAEX03001616.1. On Nov 23, 2013 this sequence version replaced XM_005617570.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGTGCACAC AGGAGGCTTT CCAAACTCAG AGGAGCCAGC TGGTGGGGCT CCTGGTCTCG 
GGGTCCCTGG AGGGCTTCGA GAGCATCCTG GACTGGCTGC TTTCCTGGGA AGTCCTCTCC 
TGGGAAGACT ACGAGGGCCT CAGCCTCCTG GGGCAGCCCC TCTCCTACTT GGCCAGGCGC 
CTCCTGGACA CCGTCTGGAA TAAGGGCACT TGGGGCTGTG AACAGCTGGT CGCAGCTGTC 
CGGGAGGCCC AGACTGACTG CCAGGCTCTA GAGCTTTCCA GCTGCTGGGA CCCCCACTCG 
CCCCACCCAG CCCGTGACCT GCAGAGTCAC CGGCCAGCCA TCGTCAGGAG ACTCTACAGC 
CACGTGGAGG ATGTGCTGAA TCTGGCGTGG GAGCAGGGTT TCATCAGCCA GTACGAACGT 
GATGAAATCA GGTTGCCCAT CTTCACATCC TCCCAGCGGG CAAGGAGGCT CCTCGATCTT 
GCCACAGTGA AGGCAAATGG GTTGGCTGCC TTCCTTCTAC GACATGTTCA GGAATTGCCA 
GTCCCGTTGG CCATGCCTTC TGAAGATGCC GCATGTAAGA AGTACATGTC CAAGCTGAGG 
ACCACGATAT CTTCTCAGTC TCGCTTTCTG AGCACCTATG ATGGGGCAGA GAATCTTTGC 
CTGGAGGAAA TATATACAGA GAATGTTCTG GAGATCCGAA CAGAAATGGG TCTGGCCAGG 
TCCCCGCAGA AGAGCCCTGC TACCCTAAGC CTGGAGGAAC TCTTCAGTAC CTGTGGCCAC 
CTCAATGAAG ATGCAGACAC TGTGCTGGTG GTGGGTGAGG CGGGCAGTGG TAAGAGCACA 
TTGCTGCAGC GAATGCACCT GCTGTGGGCT TCAGGGCGCG ACTTCCAGGA ATTTCTCTTT 
GTCTTCCCAT TCAGCTGTCG GCAGCTGCAG TGCGTGGCAA AGCCGCTCTC TGTGCAGATG 
CTGCTCTTTG AACACTGCTG TTGGCCTGAC TTTGGCCAAC AGGAAGTCTT TCAGTTCCTC 
CTTGACCACC CCAACCGTGT CCTCTTAACC TTCGATGGCT TTGATGAGTT CAGGTTCAGG 
TTCTCAGATC ATGAGCGTCA CTGCTCTCCG ACCGACCCCA CATCTGTCCA GAATCTGCTG 
TTCAACCTTC TGCAGGGCAA CTTGCTAAAG AATGCCCGCA AGGTGTTGAC CAGCCGTCCT 
GATGCAGTGT CGGCATTACT CAGGAAGTAC CTGCGCTTGG AAATCAACCT GAAGGGCTTC 
TCAGAAGAGG GCATTGAGCT GTACCTGAGG AAGTGCCACC GTGAGCCAGG GGTGGCCGAC 
CGCCTCATCC GCCTGCTCAA AACGACCTCA GCCCTGCATG GTTTGTGCCA CCTTCCTGTT 
GTCTCATGGA TGGTGTCCAA ATGCCACCAG GAGCTGTTGT TGCATGGCGG GGGGTCCCCA 
AAGACCTCCA CGGACATGTA CCTGCTGATC CTGCAGCATT TTCTGCTGCA TGCCTCCCCG 
CCAGACTCTG TCCCCCACAG TCTGGGATCC CACTTGCTTC GAGGCAGGCT CCCCACCCTC 
CTGCACCTGG GCTGGCTGGC TCTCTGGGGT CTGGGCATGT GCTGCTACGT GTTCTCCGCC 
AAGCAGCTCC AGGCAGCACA CATTGATGAT GAGGACATTT CTCTTGGCTT CCTGGTGCAT 
GCCAAGACCG TGGGGCCTGG GAGCACCACC CCCCTGGAAT TCCTGCACAT CACCTTCCAG 
TGTTTTTTTG CAGCCCTGTA CCTTGTGCTC AGCACTGACG TGTCACCGTC CTTGCTTAGA 
CAACTCTTCA TTTGTCATGG GCCGCGCAGC TCGCTGCTGG CCAGGCTTCT GCCCACAACG 
TGCGTGCCAC GCTCAGAGCG CAAGGAGGGC AGCTTGGCAG CTTTGCTGCA GGAGGCTGAG 
CCGCACAACC TCCAGATCAC GGCAGCCTTC CTGGCGGGGC TGCTGTCCCG GGAGCACCGG 
GGCCTGCTGG CCGAGTGCCA GGCCAATGAG GAGGCCCTGC TGCGGCTCCA GGGCCGGGCC 
CAGGGCTGTC TGTCCCGCAG CCTCCACCAG CACTTCCGCT CCATCCCACC AGCTGTGCCA 
GGGGAGGCCA AGAGCATGCA CGCCATGCCC GGCTTCCTCT GGCTCATCCG GAGCCTGTAC 
GAGATGCAGG AGGAGCGGCT GGCCCGGGAG GCGGTGCGTG GGCTAACGGT GGGGCACCTG 
AAGCTGACGT TCTGCGGCGT GGGCCCCCCC GAGTGCGCTG CCCTGGCCTT TGTGCTGCGG 
CACCTCCGGC GGCCTGTGGC CCTGCAGCTG GACCACAACT CTGTTGGTGA CATTGGCGTG 
GAGCAGCTGC TGCCGTGCCT CAGTGTCTGC AAGGCTCTTT ACTTGCGAGA TAACAATATC 
TCAGACCGAG GCATCTGTAA GCTCATCGAG CATGCTCTGC ACTGCGAGCA GCTGCAGAAG 
TTAGCGCTGT TCAACAACAA ATTGACCGAT GGCTGTGCAC ACTCCATGGC GAGGCTCCTT 
GCATGCAAGC AGAACTTCCT GGCTTTGAGG CTGGGGAATA ACCGTATCAC TGCTGCAGGA 
GCCCAGGCGC TGGCCGAGGG GCTCAGAGCC AACACCTCCT TGCAGTTCTT GGGGTTCTGG 
GGCAACAAGG TGGGTGATGA GGGGGCCCAG GCCTTGGCTG AAGCCTTGGG TGATCACCAG 
AGCTTGAGGT GGCTCAGCCT GGTGGGAAAC GACATTGGCA GTGTCGGGGC TCGAGCCTTA 
GCATTAATGT TGGAAAAGAA CGTGGCACTG GAAGAACTCT GCCTGGAGGA GAACCATCTC 
CAGGATGAAG GTGTGTGTTC TCTGGCCAAA GGACTTGAGA GGAATTCAAG TTTGAAAGTT 
CTGAAGCTGT CCAACAACTG CATCACCTAC CTTGGGGCAG AAGGCCTCCT GCAGGCCCTG 
GAAAAGAATG ATACCATCCT GGAAGTCTGG CTCCGAGGCA ACACTTTCTC CTTGGAGGAA 
ATGGAGAGGC TCAGCCAGAA GGACACCAGA CTGTTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273968.1
CDS	9..3050
Translation	MCTQEAFQTQRSQLVGLLVSGSLEGFESILDWLLSWEVLSWEDYEGLSLLGQPLSYLARRLLDTVWNKGTWGCEQLVAAVREAQTDCQALELSSCWDPHSPHPARDLQSHRPAIVRRLYSHVEDVLNLAWEQGFISQYERDEIRLPIFTSSQRARRLLDLATVKANGLAAFLLRHVQELPVPLAMPSEDAACKKYMSKLRTTISSQSRFLSTYDGAENLCLEEIYTENVLEIRTEMGLARSPQKSPATLSLEELFSTCGHLNEDADTVLVVGEAGSGKSTLLQRMHLLWASGRDFQEFLFVFPFSCRQLQCVAKPLSVQMLLFEHCCWPDFGQQEVFQFLLDHPNRVLLTFDGFDEFRFRFSDHERHCSPTDPTSVQNLLFNLLQGNLLKNARKVLTSRPDAVSALLRKYLRLEINLKGFSEEGIELYLRKCHREPGVADRLIRLLKTTSALHGLCHLPVVSWMVSKCHQELLLHGGGSPKTSTDMYLLILQHFLLHASPPDSVPHSLGSHLLRGRLPTLLHLGWLALWGLGMCCYVFSAKQLQAAHIDDEDISLGFLVHAKTVGPGSTTPLEFLHITFQCFFAALYLVLSTDVSPSLLRQLFICHGPRSSLLARLLPTTCVPRSERKEGSLAALLQEAEPHNLQITAAFLAGLLSREHRGLLAECQANEEALLRLQGRAQGCLSRSLHQHFRSIPPAVPGEAKSMHAMPGFLWLIRSLYEMQEERLAREAVRGLTVGHLKLTFCGVGPPECAALAFVLRHLRRPVALQLDHNSVGDIGVEQLLPCLSVCKALYLRDNNISDRGICKLIEHALHCEQLQKLALFNNKLTDGCAHSMARLLACKQNFLALRLGNNRITAAGAQALAEGLRANTSLQFLGFWGNKVGDEGAQALAEALGDHQSLRWLSLVGNDIGSVGARALALMLEKNVALEELCLEENHLQDEGVCSLAKGLERNSSLKVLKLSNNCITYLGAEGLLQALEKNDTILEVWLRGNTFSLEEMERLSQKDTRLLL

Target ORF information:

RefSeq Version	NM_001287039.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris nucleotide binding oligomerization domain containing 2 (NOD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287039.1

ORF Insert Sequence:

ATGTGCACAC AGGAGGCTTT CCAAACTCAG AGGAGCCAGC TGGTGGGGCT CCTGGTCTCG 
GGGTCCCTGG AGGGCTTCGA GAGCATCCTG GACTGGCTGC TTTCCTGGGA AGTCCTCTCC 
TGGGAAGACT ACGAGGGCCT CAGCCTCCTG GGGCAGCCCC TCTCCTACTT GGCCAGGCGC 
CTCCTGGACA CCGTCTGGAA TAAGGGCACT TGGGGCTGTG AACAGCTGGT CGCAGCTGTC 
CGGGAGGCCC AGACTGACTG CCAGGCTCTA GAGCTTTCCA GCTGCTGGGA CCCCCACTCG 
CCCCACCCAG CCCGTGACCT GCAGAGTCAC CGGCCAGCCA TCGTCAGGAG ACTCTACAGC 
CACGTGGAGG ATGTGCTGAA TCTGGCGTGG GAGCAGGGTT TCATCAGCCA GTACGAACGT 
GATGAAATCA GGTTGCCCAT CTTCACATCC TCCCAGCGGG CAAGGAGGCT CCTCGATCTT 
GCCACAGTGA AGGCAAATGG GTTGGCTGCC TTCCTTCTAC GACATGTTCA GGAATTGCCA 
GTCCCGTTGG CCATGCCTTC TGAAGATGCC GCATGTAAGA AGTACATGTC CAAGCTGAGG 
ACCACGATAT CTTCTCAGTC TCGCTTTCTG AGCACCTATG ATGGGGCAGA GAATCTTTGC 
CTGGAGGAAA TATATACAGA GAATGTTCTG GAGATCCGAA CAGAAATGGG TCTGGCCAGG 
TCCCCGCAGA AGAGCCCTGC TACCCTAAGC CTGGAGGAAC TCTTCAGTAC CTGTGGCCAC 
CTCAATGAAG ATGCAGACAC TGTGCTGGTG GTGGGTGAGG CGGGCAGTGG TAAGAGCACA 
TTGCTGCAGC GAATGCACCT GCTGTGGGCT TCAGGGCGCG ACTTCCAGGA ATTTCTCTTT 
GTCTTCCCAT TCAGCTGTCG GCAGCTGCAG TGCGTGGCAA AGCCGCTCTC TGTGCAGATG 
CTGCTCTTTG AACACTGCTG TTGGCCTGAC TTTGGCCAAC AGGAAGTCTT TCAGTTCCTC 
CTTGACCACC CCAACCGTGT CCTCTTAACC TTCGATGGCT TTGATGAGTT CAGGTTCAGG 
TTCTCAGATC ATGAGCGTCA CTGCTCTCCG ACCGACCCCA CATCTGTCCA GAATCTGCTG 
TTCAACCTTC TGCAGGGCAA CTTGCTAAAG AATGCCCGCA AGGTGTTGAC CAGCCGTCCT 
GATGCAGTGT CGGCATTACT CAGGAAGTAC CTGCGCTTGG AAATCAACCT GAAGGGCTTC 
TCAGAAGAGG GCATTGAGCT GTACCTGAGG AAGTGCCACC GTGAGCCAGG GGTGGCCGAC 
CGCCTCATCC GCCTGCTCAA AACGACCTCA GCCCTGCATG GTTTGTGCCA CCTTCCTGTT 
GTCTCATGGA TGGTGTCCAA ATGCCACCAG GAGCTGTTGT TGCATGGCGG GGGGTCCCCA 
AAGACCTCCA CGGACATGTA CCTGCTGATC CTGCAGCATT TTCTGCTGCA TGCCTCCCCG 
CCAGACTCTG TCCCCCACAG TCTGGGATCC CACTTGCTTC GAGGCAGGCT CCCCACCCTC 
CTGCACCTGG GCTGGCTGGC TCTCTGGGGT CTGGGCATGT GCTGCTACGT GTTCTCCGCC 
AAGCAGCTCC AGGCAGCACA CATTGATGAT GAGGACATTT CTCTTGGCTT CCTGGTGCAT 
GCCAAGACCG TGGGGCCTGG GAGCACCACC CCCCTGGAAT TCCTGCACAT CACCTTCCAG 
TGTTTTTTTG CAGCCCTGTA CCTTGTGCTC AGCACTGACG TGTCACCGTC CTTGCTTAGA 
CAACTCTTCA TTTGTCATGG GCCGCGCAGC TCGCTGCTGG CCAGGCTTCT GCCCACAACG 
TGCGTGCCAC GCTCAGAGCG CAAGGAGGGC AGCTTGGCAG CTTTGCTGCA GGAGGCTGAG 
CCGCACAACC TCCAGATCAC GGCAGCCTTC CTGGCGGGGC TGCTGTCCCG GGAGCACCGG 
GGCCTGCTGG CCGAGTGCCA GGCCAATGAG GAGGCCCTGC TGCGGCTCCA GGGCCGGGCC 
CAGGGCTGTC TGTCCCGCAG CCTCCACCAG CACTTCCGCT CCATCCCACC AGCTGTGCCA 
GGGGAGGCCA AGAGCATGCA CGCCATGCCC GGCTTCCTCT GGCTCATCCG GAGCCTGTAC 
GAGATGCAGG AGGAGCGGCT GGCCCGGGAG GCGGTGCGTG GGCTAACGGT GGGGCACCTG 
AAGCTGACGT TCTGCGGCGT GGGCCCCCCC GAGTGCGCTG CCCTGGCCTT TGTGCTGCGG 
CACCTCCGGC GGCCTGTGGC CCTGCAGCTG GACCACAACT CTGTTGGTGA CATTGGCGTG 
GAGCAGCTGC TGCCGTGCCT CAGTGTCTGC AAGGCTCTTT ACTTGCGAGA TAACAATATC 
TCAGACCGAG GCATCTGTAA GCTCATCGAG CATGCTCTGC ACTGCGAGCA GCTGCAGAAG 
TTAGCGCTGT TCAACAACAA ATTGACCGAT GGCTGTGCAC ACTCCATGGC GAGGCTCCTT 
GCATGCAAGC AGAACTTCCT GGCTTTGAGG CTGGGGAATA ACCGTATCAC TGCTGCAGGA 
GCCCAGGCGC TGGCCGAGGG GCTCAGAGCC AACACCTCCT TGCAGTTCTT GGGGTTCTGG 
GGCAACAAGG TGGGTGATGA GGGGGCCCAG GCCTTGGCTG AAGCCTTGGG TGATCACCAG 
AGCTTGAGGT GGCTCAGCCT GGTGGGAAAC GACATTGGCA GTGTCGGGGC TCGAGCCTTA 
GCATTAATGT TGGAAAAGAA CGTGGCACTG GAAGAACTCT GCCTGGAGGA GAACCATCTC 
CAGGATGAAG GTGTGTGTTC TCTGGCCAAA GGACTTGAGA GGAATTCAAG TTTGAAAGTT 
CTGAAGCTGT CCAACAACTG CATCACCTAC CTTGGGGCAG AAGGCCTCCT GCAGGCCCTG 
GAAAAGAATG ATACCATCCT GGAAGTCTGG CTCCGAGGCA ACACTTTCTC CTTGGAGGAA 
ATGGAGAGGC TCAGCCAGAA GGACACCAGA CTGTTGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polymorphisms of nucleotide-binding oligomerization domain 2 (NOD2) gene in miniature dachshunds with inflammatory colorectal polyps.
Veterinary immunology and immunopathology164(3-4)160-9(2015 Apr)
Igarashi H,Ohno K,Uchida E,Fujiwara-Igarashi A,Kanemoto H,Fukushima K,Uchida K,Tsujimoto H

Association between nucleotide oligomerisation domain two (Nod2) gene polymorphisms and canine inflammatory bowel disease.
Veterinary immunology and immunopathology161(1-2)32-41(2014 Sep)
Kathrani A,Lee H,White C,Catchpole B,Murphy A,German A,Werling D,Allenspach K

NOD2 mRNA expression and NFkappaB activation in dogs with lymphocytic plasmacytic colitis.
Journal of veterinary internal medicine27(3)439-44(2013 May-Jun)
Okanishi H,Hayashi K,Sakamoto Y,Sano T,Maruyama H,Kagawa Y,Watari T

Toll- and NOD-like receptor mRNA expression in canine sino-nasal aspergillosis and idiopathic lymphoplasmacytic rhinitis.
Veterinary immunology and immunopathology145(3-4)618-24(2012 Feb)
Mercier E,Peters IR,Day MJ,Clercx C,Peeters D

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NOD2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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