ADAMTS20 cDNA ORF clone, Canis lupus familiaris(dog)

The following ADAMTS20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADAMTS20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe27130 XM_847045.5
Latest version!
Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe282736 XM_022410987.1
Latest version!
Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe282737 XM_022410988.1
Latest version!
Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe282738 XM_022410989.1
Latest version!
Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe282739 XM_022410990.1
Latest version!
Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe282740 XM_022410991.1
Latest version!
Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OCe27130 XM_847045.4 Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe27130
    Clone ID Related Accession (Same CDS sequence) XM_847045.4 , XM_847045.5
    Accession Version XM_847045.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726108.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_847045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTCTTCAC AGAAGAAAAA TTACCTGCAT TCGGAAGAGT
    GATCACATGG TAGTGTCCGA TCAAAGATGT GACCATTTGC CACTTCCATT GTTTGTGACT
    GAAAGGTGTA ATACAGACTG TGAACTAAGG TGGCACATCA TTAGCAAAAG TGAATGTTCA
    TCCCATTGTG GTCAAGGATA TAGATCACTG GATGTCCACT GCATGAAGTA CTCCATTCAT
    AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGTGGTG ACCAACTTAA ACCTCCCACA
    CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGATGGC ATTATTCAGA ATGGTCCCAG
    TGTTCCAGGA GTTGTGGAGG AGGGGAAAGG TCTCGAGAAT CGTACTGTGT AAATAACTTT
    GGCCACCATG TTGCCAGCAG AGAATGCCAA GAGCTTCCCC GAGTGACAAC GGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCTACT AGTGAGTGGA GTGAGTGTCT TGTTACATGT
    GGAAAAGGAA CAAAACAAAG GCAAGTGTGG TGCCAGCTGA ATGAAGATCA TTTGAATGAT
    GGTCTCTGTA ACCCGGGTAC CAAACCTGAA TCTTTGAGAC CATGTGAGCT TCATACATGT
    GCTTCCTGGC AAGTAGGACC GTGGGGTTCT TGCACGGCCA CATGTGGACG TGGGTATCAG
    ATGCGTGCTG TTAAATGTGT CAATGAGCTG CTCAGTGCAG TGTTAGATGA CAGAGTGTGC
    CATGGAGCCA GTCGCCCAAG TGACAGGCAG GACTGTATAG TCATACCTTG CCCAATTATC
    CCTAAAATTG GGGCCACTTC ATTGCCAGCT GTTCCCATAG GAAAGATAGC CCAGTGGCGA
    CATGGTTCTT GGACCCCATG TTCTGTGTCT TGTGGACAAG GTAATCAAGC CCGTTATGTA
    AGCTGTCGTG ATGCTTATGA TGGAATAGCA GATGAATCTT ATTGTGCCCA TTTACCCCGA
    CCTGCTGAAA TATCTGTTTG TTTTAACCCT TGTGGGGAGT GGCAAACTGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCATGGAAAA ACAACTCGAC AAGTTTTATG CATCAACTAC
    CATCAGCCAA TTAATGAGAA TTACTGTGAC CCTGCAGTTC GCCCTTTGAT AGAACAAGAA
    TGTAACCTGG CAGCCTGCCC ACCCATGTAC AGTCACTTTC CAAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAGGATAA TCAAAATCAG
    GGGGTCTACC CGTCAATCAG AGGAAACCAA TGGAGAACAG GACCATGGGG ATCATGCTCC
    AGCAGCTGTG CTCGAGGTGT TCAGCGCAGA GTTGTGGTCT GCCAGGATGA AGACGGACAG
    AGTGCCACTT CCTGTGATGC AGCCTCCAGG CCCCCAGAGG CCCAGCACTG CCATTCAGGC
    CCTTGTCCAC AGTGGAGCTA CGGCAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCAA GATTCGTTAT ATGTCAATTT CCCAATGGCC AAATGTCACA AGAGCAAAAC
    TGTGAAATCT TAAACAAGCC ACCTAGTGTG GTACAATGTC ATGTGCATGC TTGCCCTGAT
    GATGCAACAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCTTGTGG TAAAGGTTTA
    AAGTACCGTG AGGTGCTTTG CATTGACCAG TTCCAAGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACTCACAAA GCTTGTAGAT CTGTCAGATG CCCTTCATGG
    AAAGCCAATA GATGGAAGGA GTGCTCTGTG ACCTGTGGCT CTGGAGTTCA GCAAAGAGAT
    GTGTACTGTA GACTCAGAGG CGTTGGTCGG GTGGCTGAAG AAATGTGTGC TCAATCCACT
    CGGCCTTATT CTCAGCGGCA ATGTTGGCAC CAGGACTGCA TACAGTACCA GTGGGTGGCA
    GGAGAGTGGC TGAACTGTTC AACTTCATGT AACCAAAAAC AGACACATCG GCAAGTCAAG
    TGCACTGATG CACAAAACAG TCAAGTGAAT GAGAGTTTCT GTGATCCTTC CACCAGGCCT
    CTTTCCATCA AAGAGTGCCC GAATCCTTCC TGCAGGTACA TTGTGGTAAC AGGAGACTCA
    TCACAGTGTG CAGGTAACTG TGGATTTAGT CACCTACAAA GGATTACATA TTGCATTGCA
    ATCTGGCCTA CTGAGAAATA TAAGCTTCAT CAACTTTGGC CTATAGACTA TCGAGAATGT
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGAGCTGTTT GCATATGGCC
    ACTTGGAAAG TGGGGAAGTG GAGCAAGTGC TCAGTGACTT GCGGAGCTGG GACAATGGAG
    AGAAGAGTAG AATGCATGGC TGAACATGGC TGGCCCAGTA ACTTATGTTT AAAGCGTTTA
    AAACCAAATG CTCAAAAGAA GTGTTACATC AATGACTGTA AAACATTCAC CAGCTGCAAA
    GAAATCCAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATGATCTTAA CATTAACGGA
    AGAATAATAA AGATCTATTG CGCAGGCATG CACTCAGAGA ACCCCAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTCTCT GAAGTGTATG GTGTTAGACT ACAAAATCCA
    TATGAATGTC CTTTTAATGG AAGTAGGAGG CAAGACTGTG CATGTAAAAA TGACTACCTG
    GCTGCTGGAC GCACTGTTTT CAGCAAAATA AGAATTGATG TCAATTCCAT GCAAATTAAA
    ACCACAGATC TTCTTTTTGC CCAGACAATA TTTGGAAAAG CAGTGCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGGCAGT TCAGCATTAA CTTGGCAGGA
    ACGGGGATGA AGATATCCAG CACAGCAAAA TGGCTTGCTC AGGGCAATTA TGCCTCTGTC
    ATCATACACA GATCACAAGA TGGAACCAAA GTCTACGGCA GATGTGGAGG GTTCTGTGGA
    AAGTGTGTTC CTCACATGAC TACTGGTCTC CCAATTCAAG GTGCTCATCA GGGAATCAAA
    TCACTGCTTA ATTTTTCCAA AGCCTTCAAA GTAAACTTAA CAAATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_852138.3
    CDS188..5998
    Translation

    Target ORF information:

    RefSeq Version XM_847045.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif, 20 (ADAMTS20), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_847045.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTCTTCAC AGAAGAAAAA TTACCTGCAT TCGGAAGAGT
    GATCACATGG TAGTGTCCGA TCAAAGATGT GACCATTTGC CACTTCCATT GTTTGTGACT
    GAAAGGTGTA ATACAGACTG TGAACTAAGG TGGCACATCA TTAGCAAAAG TGAATGTTCA
    TCCCATTGTG GTCAAGGATA TAGATCACTG GATGTCCACT GCATGAAGTA CTCCATTCAT
    AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGTGGTG ACCAACTTAA ACCTCCCACA
    CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGATGGC ATTATTCAGA ATGGTCCCAG
    TGTTCCAGGA GTTGTGGAGG AGGGGAAAGG TCTCGAGAAT CGTACTGTGT AAATAACTTT
    GGCCACCATG TTGCCAGCAG AGAATGCCAA GAGCTTCCCC GAGTGACAAC GGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCTACT AGTGAGTGGA GTGAGTGTCT TGTTACATGT
    GGAAAAGGAA CAAAACAAAG GCAAGTGTGG TGCCAGCTGA ATGAAGATCA TTTGAATGAT
    GGTCTCTGTA ACCCGGGTAC CAAACCTGAA TCTTTGAGAC CATGTGAGCT TCATACATGT
    GCTTCCTGGC AAGTAGGACC GTGGGGTTCT TGCACGGCCA CATGTGGACG TGGGTATCAG
    ATGCGTGCTG TTAAATGTGT CAATGAGCTG CTCAGTGCAG TGTTAGATGA CAGAGTGTGC
    CATGGAGCCA GTCGCCCAAG TGACAGGCAG GACTGTATAG TCATACCTTG CCCAATTATC
    CCTAAAATTG GGGCCACTTC ATTGCCAGCT GTTCCCATAG GAAAGATAGC CCAGTGGCGA
    CATGGTTCTT GGACCCCATG TTCTGTGTCT TGTGGACAAG GTAATCAAGC CCGTTATGTA
    AGCTGTCGTG ATGCTTATGA TGGAATAGCA GATGAATCTT ATTGTGCCCA TTTACCCCGA
    CCTGCTGAAA TATCTGTTTG TTTTAACCCT TGTGGGGAGT GGCAAACTGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCATGGAAAA ACAACTCGAC AAGTTTTATG CATCAACTAC
    CATCAGCCAA TTAATGAGAA TTACTGTGAC CCTGCAGTTC GCCCTTTGAT AGAACAAGAA
    TGTAACCTGG CAGCCTGCCC ACCCATGTAC AGTCACTTTC CAAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAGGATAA TCAAAATCAG
    GGGGTCTACC CGTCAATCAG AGGAAACCAA TGGAGAACAG GACCATGGGG ATCATGCTCC
    AGCAGCTGTG CTCGAGGTGT TCAGCGCAGA GTTGTGGTCT GCCAGGATGA AGACGGACAG
    AGTGCCACTT CCTGTGATGC AGCCTCCAGG CCCCCAGAGG CCCAGCACTG CCATTCAGGC
    CCTTGTCCAC AGTGGAGCTA CGGCAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCAA GATTCGTTAT ATGTCAATTT CCCAATGGCC AAATGTCACA AGAGCAAAAC
    TGTGAAATCT TAAACAAGCC ACCTAGTGTG GTACAATGTC ATGTGCATGC TTGCCCTGAT
    GATGCAACAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCTTGTGG TAAAGGTTTA
    AAGTACCGTG AGGTGCTTTG CATTGACCAG TTCCAAGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACTCACAAA GCTTGTAGAT CTGTCAGATG CCCTTCATGG
    AAAGCCAATA GATGGAAGGA GTGCTCTGTG ACCTGTGGCT CTGGAGTTCA GCAAAGAGAT
    GTGTACTGTA GACTCAGAGG CGTTGGTCGG GTGGCTGAAG AAATGTGTGC TCAATCCACT
    CGGCCTTATT CTCAGCGGCA ATGTTGGCAC CAGGACTGCA TACAGTACCA GTGGGTGGCA
    GGAGAGTGGC TGAACTGTTC AACTTCATGT AACCAAAAAC AGACACATCG GCAAGTCAAG
    TGCACTGATG CACAAAACAG TCAAGTGAAT GAGAGTTTCT GTGATCCTTC CACCAGGCCT
    CTTTCCATCA AAGAGTGCCC GAATCCTTCC TGCAGGTACA TTGTGGTAAC AGGAGACTCA
    TCACAGTGTG CAGGTAACTG TGGATTTAGT CACCTACAAA GGATTACATA TTGCATTGCA
    ATCTGGCCTA CTGAGAAATA TAAGCTTCAT CAACTTTGGC CTATAGACTA TCGAGAATGT
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGAGCTGTTT GCATATGGCC
    ACTTGGAAAG TGGGGAAGTG GAGCAAGTGC TCAGTGACTT GCGGAGCTGG GACAATGGAG
    AGAAGAGTAG AATGCATGGC TGAACATGGC TGGCCCAGTA ACTTATGTTT AAAGCGTTTA
    AAACCAAATG CTCAAAAGAA GTGTTACATC AATGACTGTA AAACATTCAC CAGCTGCAAA
    GAAATCCAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATGATCTTAA CATTAACGGA
    AGAATAATAA AGATCTATTG CGCAGGCATG CACTCAGAGA ACCCCAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTCTCT GAAGTGTATG GTGTTAGACT ACAAAATCCA
    TATGAATGTC CTTTTAATGG AAGTAGGAGG CAAGACTGTG CATGTAAAAA TGACTACCTG
    GCTGCTGGAC GCACTGTTTT CAGCAAAATA AGAATTGATG TCAATTCCAT GCAAATTAAA
    ACCACAGATC TTCTTTTTGC CCAGACAATA TTTGGAAAAG CAGTGCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGGCAGT TCAGCATTAA CTTGGCAGGA
    ACGGGGATGA AGATATCCAG CACAGCAAAA TGGCTTGCTC AGGGCAATTA TGCCTCTGTC
    ATCATACACA GATCACAAGA TGGAACCAAA GTCTACGGCA GATGTGGAGG GTTCTGTGGA
    AAGTGTGTTC CTCACATGAC TACTGGTCTC CCAATTCAAG GTGCTCATCA GGGAATCAAA
    TCACTGCTTA ATTTTTCCAA AGCCTTCAAA GTAAACTTAA CAAATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe27130
    Clone ID Related Accession (Same CDS sequence) XM_847045.4 , XM_847045.5
    Accession Version XM_847045.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5811bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_847045.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTCTTCAC AGAAGAAAAA TTACCTGCAT TCGGAAGAGT
    GATCACATGG TAGTGTCCGA TCAAAGATGT GACCATTTGC CACTTCCATT GTTTGTGACT
    GAAAGGTGTA ATACAGACTG TGAACTAAGG TGGCACATCA TTAGCAAAAG TGAATGTTCA
    TCCCATTGTG GTCAAGGATA TAGATCACTG GATGTCCACT GCATGAAGTA CTCCATTCAT
    AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGTGGTG ACCAACTTAA ACCTCCCACA
    CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGATGGC ATTATTCAGA ATGGTCCCAG
    TGTTCCAGGA GTTGTGGAGG AGGGGAAAGG TCTCGAGAAT CGTACTGTGT AAATAACTTT
    GGCCACCATG TTGCCAGCAG AGAATGCCAA GAGCTTCCCC GAGTGACAAC GGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCTACT AGTGAGTGGA GTGAGTGTCT TGTTACATGT
    GGAAAAGGAA CAAAACAAAG GCAAGTGTGG TGCCAGCTGA ATGAAGATCA TTTGAATGAT
    GGTCTCTGTA ACCCGGGTAC CAAACCTGAA TCTTTGAGAC CATGTGAGCT TCATACATGT
    GCTTCCTGGC AAGTAGGACC GTGGGGTTCT TGCACGGCCA CATGTGGACG TGGGTATCAG
    ATGCGTGCTG TTAAATGTGT CAATGAGCTG CTCAGTGCAG TGTTAGATGA CAGAGTGTGC
    CATGGAGCCA GTCGCCCAAG TGACAGGCAG GACTGTATAG TCATACCTTG CCCAATTATC
    CCTAAAATTG GGGCCACTTC ATTGCCAGCT GTTCCCATAG GAAAGATAGC CCAGTGGCGA
    CATGGTTCTT GGACCCCATG TTCTGTGTCT TGTGGACAAG GTAATCAAGC CCGTTATGTA
    AGCTGTCGTG ATGCTTATGA TGGAATAGCA GATGAATCTT ATTGTGCCCA TTTACCCCGA
    CCTGCTGAAA TATCTGTTTG TTTTAACCCT TGTGGGGAGT GGCAAACTGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCATGGAAAA ACAACTCGAC AAGTTTTATG CATCAACTAC
    CATCAGCCAA TTAATGAGAA TTACTGTGAC CCTGCAGTTC GCCCTTTGAT AGAACAAGAA
    TGTAACCTGG CAGCCTGCCC ACCCATGTAC AGTCACTTTC CAAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAGGATAA TCAAAATCAG
    GGGGTCTACC CGTCAATCAG AGGAAACCAA TGGAGAACAG GACCATGGGG ATCATGCTCC
    AGCAGCTGTG CTCGAGGTGT TCAGCGCAGA GTTGTGGTCT GCCAGGATGA AGACGGACAG
    AGTGCCACTT CCTGTGATGC AGCCTCCAGG CCCCCAGAGG CCCAGCACTG CCATTCAGGC
    CCTTGTCCAC AGTGGAGCTA CGGCAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCAA GATTCGTTAT ATGTCAATTT CCCAATGGCC AAATGTCACA AGAGCAAAAC
    TGTGAAATCT TAAACAAGCC ACCTAGTGTG GTACAATGTC ATGTGCATGC TTGCCCTGAT
    GATGCAACAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCTTGTGG TAAAGGTTTA
    AAGTACCGTG AGGTGCTTTG CATTGACCAG TTCCAAGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACTCACAAA GCTTGTAGAT CTGTCAGATG CCCTTCATGG
    AAAGCCAATA GATGGAAGGA GTGCTCTGTG ACCTGTGGCT CTGGAGTTCA GCAAAGAGAT
    GTGTACTGTA GACTCAGAGG CGTTGGTCGG GTGGCTGAAG AAATGTGTGC TCAATCCACT
    CGGCCTTATT CTCAGCGGCA ATGTTGGCAC CAGGACTGCA TACAGTACCA GTGGGTGGCA
    GGAGAGTGGC TGAACTGTTC AACTTCATGT AACCAAAAAC AGACACATCG GCAAGTCAAG
    TGCACTGATG CACAAAACAG TCAAGTGAAT GAGAGTTTCT GTGATCCTTC CACCAGGCCT
    CTTTCCATCA AAGAGTGCCC GAATCCTTCC TGCAGGTACA TTGTGGTAAC AGGAGACTCA
    TCACAGTGTG CAGGTAACTG TGGATTTAGT CACCTACAAA GGATTACATA TTGCATTGCA
    ATCTGGCCTA CTGAGAAATA TAAGCTTCAT CAACTTTGGC CTATAGACTA TCGAGAATGT
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGAGCTGTTT GCATATGGCC
    ACTTGGAAAG TGGGGAAGTG GAGCAAGTGC TCAGTGACTT GCGGAGCTGG GACAATGGAG
    AGAAGAGTAG AATGCATGGC TGAACATGGC TGGCCCAGTA ACTTATGTTT AAAGCGTTTA
    AAACCAAATG CTCAAAAGAA GTGTTACATC AATGACTGTA AAACATTCAC CAGCTGCAAA
    GAAATCCAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATGATCTTAA CATTAACGGA
    AGAATAATAA AGATCTATTG CGCAGGCATG CACTCAGAGA ACCCCAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTCTCT GAAGTGTATG GTGTTAGACT ACAAAATCCA
    TATGAATGTC CTTTTAATGG AAGTAGGAGG CAAGACTGTG CATGTAAAAA TGACTACCTG
    GCTGCTGGAC GCACTGTTTT CAGCAAAATA AGAATTGATG TCAATTCCAT GCAAATTAAA
    ACCACAGATC TTCTTTTTGC CCAGACAATA TTTGGAAAAG CAGTGCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGGCAGT TCAGCATTAA CTTGGCAGGA
    ACGGGGATGA AGATATCCAG CACAGCAAAA TGGCTTGCTC AGGGCAATTA TGCCTCTGTC
    ATCATACACA GATCACAAGA TGGAACCAAA GTCTACGGCA GATGTGGAGG GTTCTGTGGA
    AAGTGTGTTC CTCACATGAC TACTGGTCTC CCAATTCAAG GTGCTCATCA GGGAATCAAA
    TCACTGCTTA ATTTTTCCAA AGCCTTCAAA GTAAACTTAA CAAATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_852138.3
    CDS72..5882
    Translation

    Target ORF information:

    RefSeq Version XM_847045.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_847045.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTCTTCAC AGAAGAAAAA TTACCTGCAT TCGGAAGAGT
    GATCACATGG TAGTGTCCGA TCAAAGATGT GACCATTTGC CACTTCCATT GTTTGTGACT
    GAAAGGTGTA ATACAGACTG TGAACTAAGG TGGCACATCA TTAGCAAAAG TGAATGTTCA
    TCCCATTGTG GTCAAGGATA TAGATCACTG GATGTCCACT GCATGAAGTA CTCCATTCAT
    AAAGGACAGA CTGTTCCAGT AGATGACCAC TACTGTGGTG ACCAACTTAA ACCTCCCACA
    CGAGAACCCT GCCATGGTGA CTGTGTCTTA ACAAGATGGC ATTATTCAGA ATGGTCCCAG
    TGTTCCAGGA GTTGTGGAGG AGGGGAAAGG TCTCGAGAAT CGTACTGTGT AAATAACTTT
    GGCCACCATG TTGCCAGCAG AGAATGCCAA GAGCTTCCCC GAGTGACAAC GGAGAATTGC
    AATGAATTTT CCTGTCCCAG TTGGGCTACT AGTGAGTGGA GTGAGTGTCT TGTTACATGT
    GGAAAAGGAA CAAAACAAAG GCAAGTGTGG TGCCAGCTGA ATGAAGATCA TTTGAATGAT
    GGTCTCTGTA ACCCGGGTAC CAAACCTGAA TCTTTGAGAC CATGTGAGCT TCATACATGT
    GCTTCCTGGC AAGTAGGACC GTGGGGTTCT TGCACGGCCA CATGTGGACG TGGGTATCAG
    ATGCGTGCTG TTAAATGTGT CAATGAGCTG CTCAGTGCAG TGTTAGATGA CAGAGTGTGC
    CATGGAGCCA GTCGCCCAAG TGACAGGCAG GACTGTATAG TCATACCTTG CCCAATTATC
    CCTAAAATTG GGGCCACTTC ATTGCCAGCT GTTCCCATAG GAAAGATAGC CCAGTGGCGA
    CATGGTTCTT GGACCCCATG TTCTGTGTCT TGTGGACAAG GTAATCAAGC CCGTTATGTA
    AGCTGTCGTG ATGCTTATGA TGGAATAGCA GATGAATCTT ATTGTGCCCA TTTACCCCGA
    CCTGCTGAAA TATCTGTTTG TTTTAACCCT TGTGGGGAGT GGCAAACTGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCATGGAAAA ACAACTCGAC AAGTTTTATG CATCAACTAC
    CATCAGCCAA TTAATGAGAA TTACTGTGAC CCTGCAGTTC GCCCTTTGAT AGAACAAGAA
    TGTAACCTGG CAGCCTGCCC ACCCATGTAC AGTCACTTTC CAAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAGGATAA TCAAAATCAG
    GGGGTCTACC CGTCAATCAG AGGAAACCAA TGGAGAACAG GACCATGGGG ATCATGCTCC
    AGCAGCTGTG CTCGAGGTGT TCAGCGCAGA GTTGTGGTCT GCCAGGATGA AGACGGACAG
    AGTGCCACTT CCTGTGATGC AGCCTCCAGG CCCCCAGAGG CCCAGCACTG CCATTCAGGC
    CCTTGTCCAC AGTGGAGCTA CGGCAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCAA GATTCGTTAT ATGTCAATTT CCCAATGGCC AAATGTCACA AGAGCAAAAC
    TGTGAAATCT TAAACAAGCC ACCTAGTGTG GTACAATGTC ATGTGCATGC TTGCCCTGAT
    GATGCAACAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCTTGTGG TAAAGGTTTA
    AAGTACCGTG AGGTGCTTTG CATTGACCAG TTCCAAGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACTCACAAA GCTTGTAGAT CTGTCAGATG CCCTTCATGG
    AAAGCCAATA GATGGAAGGA GTGCTCTGTG ACCTGTGGCT CTGGAGTTCA GCAAAGAGAT
    GTGTACTGTA GACTCAGAGG CGTTGGTCGG GTGGCTGAAG AAATGTGTGC TCAATCCACT
    CGGCCTTATT CTCAGCGGCA ATGTTGGCAC CAGGACTGCA TACAGTACCA GTGGGTGGCA
    GGAGAGTGGC TGAACTGTTC AACTTCATGT AACCAAAAAC AGACACATCG GCAAGTCAAG
    TGCACTGATG CACAAAACAG TCAAGTGAAT GAGAGTTTCT GTGATCCTTC CACCAGGCCT
    CTTTCCATCA AAGAGTGCCC GAATCCTTCC TGCAGGTACA TTGTGGTAAC AGGAGACTCA
    TCACAGTGTG CAGGTAACTG TGGATTTAGT CACCTACAAA GGATTACATA TTGCATTGCA
    ATCTGGCCTA CTGAGAAATA TAAGCTTCAT CAACTTTGGC CTATAGACTA TCGAGAATGT
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGAGCTGTTT GCATATGGCC
    ACTTGGAAAG TGGGGAAGTG GAGCAAGTGC TCAGTGACTT GCGGAGCTGG GACAATGGAG
    AGAAGAGTAG AATGCATGGC TGAACATGGC TGGCCCAGTA ACTTATGTTT AAAGCGTTTA
    AAACCAAATG CTCAAAAGAA GTGTTACATC AATGACTGTA AAACATTCAC CAGCTGCAAA
    GAAATCCAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATGATCTTAA CATTAACGGA
    AGAATAATAA AGATCTATTG CGCAGGCATG CACTCAGAGA ACCCCAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTCTCT GAAGTGTATG GTGTTAGACT ACAAAATCCA
    TATGAATGTC CTTTTAATGG AAGTAGGAGG CAAGACTGTG CATGTAAAAA TGACTACCTG
    GCTGCTGGAC GCACTGTTTT CAGCAAAATA AGAATTGATG TCAATTCCAT GCAAATTAAA
    ACCACAGATC TTCTTTTTGC CCAGACAATA TTTGGAAAAG CAGTGCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGGCAGT TCAGCATTAA CTTGGCAGGA
    ACGGGGATGA AGATATCCAG CACAGCAAAA TGGCTTGCTC AGGGCAATTA TGCCTCTGTC
    ATCATACACA GATCACAAGA TGGAACCAAA GTCTACGGCA GATGTGGAGG GTTCTGTGGA
    AAGTGTGTTC CTCACATGAC TACTGGTCTC CCAATTCAAG GTGCTCATCA GGGAATCAAA
    TCACTGCTTA ATTTTTCCAA AGCCTTCAAA GTAAACTTAA CAAATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282736
    Clone ID Related Accession (Same CDS sequence) XM_022410987.1
    Accession Version XM_022410987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGGA CTGTATAGTC ATACCTTGCC CAATTATCCC TAAAATTGGG
    GCCACTTCAT TGCCAGCTGT TCCCATAGGA AAGATAGCCC AGTGGCGACA TGGTTCTTGG
    ACCCCATGTT CTGTGTCTTG TGGACAAGGT AATCAAGCCC GTTATGTAAG CTGTCGTGAT
    GCTTATGATG GAATAGCAGA TGAATCTTAT TGTGCCCATT TACCCCGACC TGCTGAAATA
    TCTGTTTGTT TTAACCCTTG TGGGGAGTGG CAAACTGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC ATGGAAAAAC AACTCGACAA GTTTTATGCA TCAACTACCA TCAGCCAATT
    AATGAGAATT ACTGTGACCC TGCAGTTCGC CCTTTGATAG AACAAGAATG TAACCTGGCA
    GCCTGCCCAC CCATGTACAG TCACTTTCCA AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAGGATAATC AAAATCAGGG GGTCTACCCG
    TCAATCAGAG GAAACCAATG GAGAACAGGA CCATGGGGAT CATGCTCCAG CAGCTGTGCT
    CGAGGTGTTC AGCGCAGAGT TGTGGTCTGC CAGGATGAAG ACGGACAGAG TGCCACTTCC
    TGTGATGCAG CCTCCAGGCC CCCAGAGGCC CAGCACTGCC ATTCAGGCCC TTGTCCACAG
    TGGAGCTACG GCAGCTGGGG AGAATGTACA CAAACATGTG GAGGAGGAAT AAAATCAAGA
    TTCGTTATAT GTCAATTTCC CAATGGCCAA ATGTCACAAG AGCAAAACTG TGAAATCTTA
    AACAAGCCAC CTAGTGTGGT ACAATGTCAT GTGCATGCTT GCCCTGATGA TGCAACATGG
    CATCGGGGAC CATGGAAATC GTGCTCAGCT TCTTGTGGTA AAGGTTTAAA GTACCGTGAG
    GTGCTTTGCA TTGACCAGTT CCAAGGGAAA TTAGAAGAAA AATATTGCAG TCATCTACAG
    AAACCTCGAA CTCACAAAGC TTGTAGATCT GTCAGATGCC CTTCATGGAA AGCCAATAGA
    TGGAAGGAGT GCTCTGTGAC CTGTGGCTCT GGAGTTCAGC AAAGAGATGT GTACTGTAGA
    CTCAGAGGCG TTGGTCGGGT GGCTGAAGAA ATGTGTGCTC AATCCACTCG GCCTTATTCT
    CAGCGGCAAT GTTGGCACCA GGACTGCATA CAGTACCAGT GGGTGGCAGG AGAGTGGCTG
    AACTGTTCAA CTTCATGTAA CCAAAAACAG ACACATCGGC AAGTCAAGTG CACTGATGCA
    CAAAACAGTC AAGTGAATGA GAGTTTCTGT GATCCTTCCA CCAGGCCTCT TTCCATCAAA
    GAGTGCCCGA ATCCTTCCTG CAGGTACATT GTGGTAACAG GAGACTCATC ACAGTGTGCA
    GGTAACTGTG GATTTAGTCA CCTACAAAGG ATTACATATT GCATTGCAAT CTGGCCTACT
    GAGAAATATA AGCTTCATCA ACTTTGGCCT ATAGACTATC GAGAATGTCC TGTGCTGCCT
    TCCCCTCAGG TTTACAAATG CAATTTTAGG AGCTGTTTGC ATATGGCCAC TTGGAAAGTG
    GGGAAGTGGA GCAAGTGCTC AGTGACTTGC GGAGCTGGGA CAATGGAGAG AAGAGTAGAA
    TGCATGGCTG AACATGGCTG GCCCAGTAAC TTATGTTTAA AGCGTTTAAA ACCAAATGCT
    CAAAAGAAGT GTTACATCAA TGACTGTAAA ACATTCACCA GCTGCAAAGA AATCCAAGTG
    AAAAACAACA TTACCAAGGA TGGTGACTAT GATCTTAACA TTAACGGAAG AATAATAAAG
    ATCTATTGCG CAGGCATGCA CTCAGAGAAC CCCAAGGAAT ATATATCATT GGTCAAAGGT
    GAAGAAGACA ACTTCTCTGA AGTGTATGGT GTTAGACTAC AAAATCCATA TGAATGTCCT
    TTTAATGGAA GTAGGAGGCA AGACTGTGCA TGTAAAAATG ACTACCTGGC TGCTGGACGC
    ACTGTTTTCA GCAAAATAAG AATTGATGTC AATTCCATGC AAATTAAAAC CACAGATCTT
    CTTTTTGCCC AGACAATATT TGGAAAAGCA GTGCCATTTG CCACAGCTGG AGATTGCTAT
    AGTGCTGCCA GATGCCCACA GGGGCAGTTC AGCATTAACT TGGCAGGAAC GGGGATGAAG
    ATATCCAGCA CAGCAAAATG GCTTGCTCAG GGCAATTATG CCTCTGTCAT CATACACAGA
    TCACAAGATG GAACCAAAGT CTACGGCAGA TGTGGAGGGT TCTGTGGAAA GTGTGTTCCT
    CACATGACTA CTGGTCTCCC AATTCAAGGT GCTCATCAGG GAATCAAATC ACTGCTTAAT
    TTTTCCAAAG CCTTCAAAGT AAACTTAACA AATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266695.1
    CDS72..5930
    Translation

    Target ORF information:

    RefSeq Version XM_022410987.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGGA CTGTATAGTC ATACCTTGCC CAATTATCCC TAAAATTGGG
    GCCACTTCAT TGCCAGCTGT TCCCATAGGA AAGATAGCCC AGTGGCGACA TGGTTCTTGG
    ACCCCATGTT CTGTGTCTTG TGGACAAGGT AATCAAGCCC GTTATGTAAG CTGTCGTGAT
    GCTTATGATG GAATAGCAGA TGAATCTTAT TGTGCCCATT TACCCCGACC TGCTGAAATA
    TCTGTTTGTT TTAACCCTTG TGGGGAGTGG CAAACTGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC ATGGAAAAAC AACTCGACAA GTTTTATGCA TCAACTACCA TCAGCCAATT
    AATGAGAATT ACTGTGACCC TGCAGTTCGC CCTTTGATAG AACAAGAATG TAACCTGGCA
    GCCTGCCCAC CCATGTACAG TCACTTTCCA AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAGGATAATC AAAATCAGGG GGTCTACCCG
    TCAATCAGAG GAAACCAATG GAGAACAGGA CCATGGGGAT CATGCTCCAG CAGCTGTGCT
    CGAGGTGTTC AGCGCAGAGT TGTGGTCTGC CAGGATGAAG ACGGACAGAG TGCCACTTCC
    TGTGATGCAG CCTCCAGGCC CCCAGAGGCC CAGCACTGCC ATTCAGGCCC TTGTCCACAG
    TGGAGCTACG GCAGCTGGGG AGAATGTACA CAAACATGTG GAGGAGGAAT AAAATCAAGA
    TTCGTTATAT GTCAATTTCC CAATGGCCAA ATGTCACAAG AGCAAAACTG TGAAATCTTA
    AACAAGCCAC CTAGTGTGGT ACAATGTCAT GTGCATGCTT GCCCTGATGA TGCAACATGG
    CATCGGGGAC CATGGAAATC GTGCTCAGCT TCTTGTGGTA AAGGTTTAAA GTACCGTGAG
    GTGCTTTGCA TTGACCAGTT CCAAGGGAAA TTAGAAGAAA AATATTGCAG TCATCTACAG
    AAACCTCGAA CTCACAAAGC TTGTAGATCT GTCAGATGCC CTTCATGGAA AGCCAATAGA
    TGGAAGGAGT GCTCTGTGAC CTGTGGCTCT GGAGTTCAGC AAAGAGATGT GTACTGTAGA
    CTCAGAGGCG TTGGTCGGGT GGCTGAAGAA ATGTGTGCTC AATCCACTCG GCCTTATTCT
    CAGCGGCAAT GTTGGCACCA GGACTGCATA CAGTACCAGT GGGTGGCAGG AGAGTGGCTG
    AACTGTTCAA CTTCATGTAA CCAAAAACAG ACACATCGGC AAGTCAAGTG CACTGATGCA
    CAAAACAGTC AAGTGAATGA GAGTTTCTGT GATCCTTCCA CCAGGCCTCT TTCCATCAAA
    GAGTGCCCGA ATCCTTCCTG CAGGTACATT GTGGTAACAG GAGACTCATC ACAGTGTGCA
    GGTAACTGTG GATTTAGTCA CCTACAAAGG ATTACATATT GCATTGCAAT CTGGCCTACT
    GAGAAATATA AGCTTCATCA ACTTTGGCCT ATAGACTATC GAGAATGTCC TGTGCTGCCT
    TCCCCTCAGG TTTACAAATG CAATTTTAGG AGCTGTTTGC ATATGGCCAC TTGGAAAGTG
    GGGAAGTGGA GCAAGTGCTC AGTGACTTGC GGAGCTGGGA CAATGGAGAG AAGAGTAGAA
    TGCATGGCTG AACATGGCTG GCCCAGTAAC TTATGTTTAA AGCGTTTAAA ACCAAATGCT
    CAAAAGAAGT GTTACATCAA TGACTGTAAA ACATTCACCA GCTGCAAAGA AATCCAAGTG
    AAAAACAACA TTACCAAGGA TGGTGACTAT GATCTTAACA TTAACGGAAG AATAATAAAG
    ATCTATTGCG CAGGCATGCA CTCAGAGAAC CCCAAGGAAT ATATATCATT GGTCAAAGGT
    GAAGAAGACA ACTTCTCTGA AGTGTATGGT GTTAGACTAC AAAATCCATA TGAATGTCCT
    TTTAATGGAA GTAGGAGGCA AGACTGTGCA TGTAAAAATG ACTACCTGGC TGCTGGACGC
    ACTGTTTTCA GCAAAATAAG AATTGATGTC AATTCCATGC AAATTAAAAC CACAGATCTT
    CTTTTTGCCC AGACAATATT TGGAAAAGCA GTGCCATTTG CCACAGCTGG AGATTGCTAT
    AGTGCTGCCA GATGCCCACA GGGGCAGTTC AGCATTAACT TGGCAGGAAC GGGGATGAAG
    ATATCCAGCA CAGCAAAATG GCTTGCTCAG GGCAATTATG CCTCTGTCAT CATACACAGA
    TCACAAGATG GAACCAAAGT CTACGGCAGA TGTGGAGGGT TCTGTGGAAA GTGTGTTCCT
    CACATGACTA CTGGTCTCCC AATTCAAGGT GCTCATCAGG GAATCAAATC ACTGCTTAAT
    TTTTCCAAAG CCTTCAAAGT AAACTTAACA AATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282737
    Clone ID Related Accession (Same CDS sequence) XM_022410988.1
    Accession Version XM_022410988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5796bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GTTGCCATAA AAGATCGCTG TAAACTGTAT
    TGTCGGGTTG CCGGAACCAC TAACTTCTAC CAGTTGAAGG ATAGGGTTGC TGATGGTACT
    CCTTGTGGAA CCGAAACTAA TGATATCTGT GTTCAAGGCC TCTGTCGGCA AGCTGGCTGT
    GATCATGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GAGTGTGTGG TGGGGATAAC
    TCTTCATGCA GGACAATGAC AGGTGTCTTC AACAGTGCTC ATTATGGTTA TAATGTTGTT
    GTCAAGATTC CTGCAGGAGC AACAAACATT GATATTCTTC AGCATAGCTA TTCTGGAAAA
    CCAGAAGATG ACAATTACCT TGCATTATCT GACACTCAAG GGAATTTTCT TTTGAATGGG
    AATTTTGTTG TAAGTATGTC AAAAAAAGAA ATCAACATAC AAGGAGCTAT TTTTGAATAC
    AGTGGTTCAA ACAGCTCAAT TGAAAGAATT AATAGTACTG ATCGGCTGGA AGAAGAAGTT
    GTTTTGCAGG TTTTATGTGT GGGTAATTTA TACAACCCTG ATGTACGATA TTCCTTCAAT
    ATCCCCGTGG AAGAGAAGAA CGACCTAGTC ACATGGGATC CGTATGGACC ATGGCAAGAC
    TGCACCAAGA TGTGTCAAGG TACACTGGTG TTGAGTGAAA GAGACAGAAG CTTGCCTGTG
    CATGCTGGTC TTCACAGAAG AAAAATTACC TGCATTCGGA AGAGTGATCA CATGGTAGTG
    TCCGATCAAA GATGTGACCA TTTGCCACTT CCATTGTTTG TGACTGAAAG GTGTAATACA
    GACTGTGAAC TAAGGTGGCA CATCATTAGC AAAAGTGAAT GTTCATCCCA TTGTGGTCAA
    GGATATAGAT CACTGGATGT CCACTGCATG AAGTACTCCA TTCATAAAGG ACAGACTGTT
    CCAGTAGATG ACCACTACTG TGGTGACCAA CTTAAACCTC CCACACGAGA ACCCTGCCAT
    GGTGACTGTG TCTTAACAAG ATGGCATTAT TCAGAATGGT CCCAGTGTTC CAGGAGTTGT
    GGAGGAGGGG AAAGGTCTCG AGAATCGTAC TGTGTAAATA ACTTTGGCCA CCATGTTGCC
    AGCAGAGAAT GCCAAGAGCT TCCCCGAGTG ACAACGGAGA ATTGCAATGA ATTTTCCTGT
    CCCAGTTGGG CTACTAGTGA GTGGAGTGAG TGTCTTGTTA CATGTGGAAA AGGAACAAAA
    CAAAGGCAAG TGTGGTGCCA GCTGAATGAA GATCATTTGA ATGATGGTCT CTGTAACCCG
    GGTACCAAAC CTGAATCTTT GAGACCATGT GAGCTTCATA CATGTGCTTC CTGGCAAGTA
    GGACCGTGGG GTTCTTGCAC GGCCACATGT GGACGTGGGT ATCAGATGCG TGCTGTTAAA
    TGTGTCAATG AGCTGCTCAG TGCAGTGTTA GATGACAGAG TGTGCCATGG AGCCAGTCGC
    CCAAGTGACA GGCAGGACTG TATAGTCATA CCTTGCCCAA TTATCCCTAA AATTGGGGCC
    ACTTCATTGC CAGCTGTTCC CATAGGAAAG ATAGCCCAGT GGCGACATGG TTCTTGGACC
    CCATGTTCTG TGTCTTGTGG ACAAGGTAAT CAAGCCCGTT ATGTAAGCTG TCGTGATGCT
    TATGATGGAA TAGCAGATGA ATCTTATTGT GCCCATTTAC CCCGACCTGC TGAAATATCT
    GTTTGTTTTA ACCCTTGTGG GGAGTGGCAA ACTGGGAATT GGTCACCTTG TTCAGCTTCC
    TGTGGCCATG GAAAAACAAC TCGACAAGTT TTATGCATCA ACTACCATCA GCCAATTAAT
    GAGAATTACT GTGACCCTGC AGTTCGCCCT TTGATAGAAC AAGAATGTAA CCTGGCAGCC
    TGCCCACCCA TGTACAGTCA CTTTCCAAGT TCCTCTGAGC AGCCAAGCCA TTTTCCAGGC
    AGGAATTTTC CATTAACTCA CAAACCAGAG GATAATCAAA ATCAGGGGGT CTACCCGTCA
    ATCAGAGGAA ACCAATGGAG AACAGGACCA TGGGGATCAT GCTCCAGCAG CTGTGCTCGA
    GGTGTTCAGC GCAGAGTTGT GGTCTGCCAG GATGAAGACG GACAGAGTGC CACTTCCTGT
    GATGCAGCCT CCAGGCCCCC AGAGGCCCAG CACTGCCATT CAGGCCCTTG TCCACAGTGG
    AGCTACGGCA GCTGGGGAGA ATGTACACAA ACATGTGGAG GAGGAATAAA ATCAAGATTC
    GTTATATGTC AATTTCCCAA TGGCCAAATG TCACAAGAGC AAAACTGTGA AATCTTAAAC
    AAGCCACCTA GTGTGGTACA ATGTCATGTG CATGCTTGCC CTGATGATGC AACATGGCAT
    CGGGGACCAT GGAAATCGTG CTCAGCTTCT TGTGGTAAAG GTTTAAAGTA CCGTGAGGTG
    CTTTGCATTG ACCAGTTCCA AGGGAAATTA GAAGAAAAAT ATTGCAGTCA TCTACAGAAA
    CCTCGAACTC ACAAAGCTTG TAGATCTGTC AGATGCCCTT CATGGAAAGC CAATAGATGG
    AAGGAGTGCT CTGTGACCTG TGGCTCTGGA GTTCAGCAAA GAGATGTGTA CTGTAGACTC
    AGAGGCGTTG GTCGGGTGGC TGAAGAAATG TGTGCTCAAT CCACTCGGCC TTATTCTCAG
    CGGCAATGTT GGCACCAGGA CTGCATACAG TACCAGTGGG TGGCAGGAGA GTGGCTGAAC
    TGTTCAACTT CATGTAACCA AAAACAGACA CATCGGCAAG TCAAGTGCAC TGATGCACAA
    AACAGTCAAG TGAATGAGAG TTTCTGTGAT CCTTCCACCA GGCCTCTTTC CATCAAAGAG
    TGCCCGAATC CTTCCTGCAG GTACATTGTG GTAACAGGAG ACTCATCACA GTGTGCAGGT
    AACTGTGGAT TTAGTCACCT ACAAAGGATT ACATATTGCA TTGCAATCTG GCCTACTGAG
    AAATATAAGC TTCATCAACT TTGGCCTATA GACTATCGAG AATGTCCTGT GCTGCCTTCC
    CCTCAGGTTT ACAAATGCAA TTTTAGGAGC TGTTTGCATA TGGCCACTTG GAAAGTGGGG
    AAGTGGAGCA AGTGCTCAGT GACTTGCGGA GCTGGGACAA TGGAGAGAAG AGTAGAATGC
    ATGGCTGAAC ATGGCTGGCC CAGTAACTTA TGTTTAAAGC GTTTAAAACC AAATGCTCAA
    AAGAAGTGTT ACATCAATGA CTGTAAAACA TTCACCAGCT GCAAAGAAAT CCAAGTGAAA
    AACAACATTA CCAAGGATGG TGACTATGAT CTTAACATTA ACGGAAGAAT AATAAAGATC
    TATTGCGCAG GCATGCACTC AGAGAACCCC AAGGAATATA TATCATTGGT CAAAGGTGAA
    GAAGACAACT TCTCTGAAGT GTATGGTGTT AGACTACAAA ATCCATATGA ATGTCCTTTT
    AATGGAAGTA GGAGGCAAGA CTGTGCATGT AAAAATGACT ACCTGGCTGC TGGACGCACT
    GTTTTCAGCA AAATAAGAAT TGATGTCAAT TCCATGCAAA TTAAAACCAC AGATCTTCTT
    TTTGCCCAGA CAATATTTGG AAAAGCAGTG CCATTTGCCA CAGCTGGAGA TTGCTATAGT
    GCTGCCAGAT GCCCACAGGG GCAGTTCAGC ATTAACTTGG CAGGAACGGG GATGAAGATA
    TCCAGCACAG CAAAATGGCT TGCTCAGGGC AATTATGCCT CTGTCATCAT ACACAGATCA
    CAAGATGGAA CCAAAGTCTA CGGCAGATGT GGAGGGTTCT GTGGAAAGTG TGTTCCTCAC
    ATGACTACTG GTCTCCCAAT TCAAGGTGCT CATCAGGGAA TCAAATCACT GCTTAATTTT
    TCCAAAGCCT TCAAAGTAAA CTTAACAAAT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266696.1
    CDS72..5867
    Translation

    Target ORF information:

    RefSeq Version XM_022410988.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GTTGCCATAA AAGATCGCTG TAAACTGTAT
    TGTCGGGTTG CCGGAACCAC TAACTTCTAC CAGTTGAAGG ATAGGGTTGC TGATGGTACT
    CCTTGTGGAA CCGAAACTAA TGATATCTGT GTTCAAGGCC TCTGTCGGCA AGCTGGCTGT
    GATCATGTGT TAAACTCCAA GGCCAGGAGA GACAAATGTG GAGTGTGTGG TGGGGATAAC
    TCTTCATGCA GGACAATGAC AGGTGTCTTC AACAGTGCTC ATTATGGTTA TAATGTTGTT
    GTCAAGATTC CTGCAGGAGC AACAAACATT GATATTCTTC AGCATAGCTA TTCTGGAAAA
    CCAGAAGATG ACAATTACCT TGCATTATCT GACACTCAAG GGAATTTTCT TTTGAATGGG
    AATTTTGTTG TAAGTATGTC AAAAAAAGAA ATCAACATAC AAGGAGCTAT TTTTGAATAC
    AGTGGTTCAA ACAGCTCAAT TGAAAGAATT AATAGTACTG ATCGGCTGGA AGAAGAAGTT
    GTTTTGCAGG TTTTATGTGT GGGTAATTTA TACAACCCTG ATGTACGATA TTCCTTCAAT
    ATCCCCGTGG AAGAGAAGAA CGACCTAGTC ACATGGGATC CGTATGGACC ATGGCAAGAC
    TGCACCAAGA TGTGTCAAGG TACACTGGTG TTGAGTGAAA GAGACAGAAG CTTGCCTGTG
    CATGCTGGTC TTCACAGAAG AAAAATTACC TGCATTCGGA AGAGTGATCA CATGGTAGTG
    TCCGATCAAA GATGTGACCA TTTGCCACTT CCATTGTTTG TGACTGAAAG GTGTAATACA
    GACTGTGAAC TAAGGTGGCA CATCATTAGC AAAAGTGAAT GTTCATCCCA TTGTGGTCAA
    GGATATAGAT CACTGGATGT CCACTGCATG AAGTACTCCA TTCATAAAGG ACAGACTGTT
    CCAGTAGATG ACCACTACTG TGGTGACCAA CTTAAACCTC CCACACGAGA ACCCTGCCAT
    GGTGACTGTG TCTTAACAAG ATGGCATTAT TCAGAATGGT CCCAGTGTTC CAGGAGTTGT
    GGAGGAGGGG AAAGGTCTCG AGAATCGTAC TGTGTAAATA ACTTTGGCCA CCATGTTGCC
    AGCAGAGAAT GCCAAGAGCT TCCCCGAGTG ACAACGGAGA ATTGCAATGA ATTTTCCTGT
    CCCAGTTGGG CTACTAGTGA GTGGAGTGAG TGTCTTGTTA CATGTGGAAA AGGAACAAAA
    CAAAGGCAAG TGTGGTGCCA GCTGAATGAA GATCATTTGA ATGATGGTCT CTGTAACCCG
    GGTACCAAAC CTGAATCTTT GAGACCATGT GAGCTTCATA CATGTGCTTC CTGGCAAGTA
    GGACCGTGGG GTTCTTGCAC GGCCACATGT GGACGTGGGT ATCAGATGCG TGCTGTTAAA
    TGTGTCAATG AGCTGCTCAG TGCAGTGTTA GATGACAGAG TGTGCCATGG AGCCAGTCGC
    CCAAGTGACA GGCAGGACTG TATAGTCATA CCTTGCCCAA TTATCCCTAA AATTGGGGCC
    ACTTCATTGC CAGCTGTTCC CATAGGAAAG ATAGCCCAGT GGCGACATGG TTCTTGGACC
    CCATGTTCTG TGTCTTGTGG ACAAGGTAAT CAAGCCCGTT ATGTAAGCTG TCGTGATGCT
    TATGATGGAA TAGCAGATGA ATCTTATTGT GCCCATTTAC CCCGACCTGC TGAAATATCT
    GTTTGTTTTA ACCCTTGTGG GGAGTGGCAA ACTGGGAATT GGTCACCTTG TTCAGCTTCC
    TGTGGCCATG GAAAAACAAC TCGACAAGTT TTATGCATCA ACTACCATCA GCCAATTAAT
    GAGAATTACT GTGACCCTGC AGTTCGCCCT TTGATAGAAC AAGAATGTAA CCTGGCAGCC
    TGCCCACCCA TGTACAGTCA CTTTCCAAGT TCCTCTGAGC AGCCAAGCCA TTTTCCAGGC
    AGGAATTTTC CATTAACTCA CAAACCAGAG GATAATCAAA ATCAGGGGGT CTACCCGTCA
    ATCAGAGGAA ACCAATGGAG AACAGGACCA TGGGGATCAT GCTCCAGCAG CTGTGCTCGA
    GGTGTTCAGC GCAGAGTTGT GGTCTGCCAG GATGAAGACG GACAGAGTGC CACTTCCTGT
    GATGCAGCCT CCAGGCCCCC AGAGGCCCAG CACTGCCATT CAGGCCCTTG TCCACAGTGG
    AGCTACGGCA GCTGGGGAGA ATGTACACAA ACATGTGGAG GAGGAATAAA ATCAAGATTC
    GTTATATGTC AATTTCCCAA TGGCCAAATG TCACAAGAGC AAAACTGTGA AATCTTAAAC
    AAGCCACCTA GTGTGGTACA ATGTCATGTG CATGCTTGCC CTGATGATGC AACATGGCAT
    CGGGGACCAT GGAAATCGTG CTCAGCTTCT TGTGGTAAAG GTTTAAAGTA CCGTGAGGTG
    CTTTGCATTG ACCAGTTCCA AGGGAAATTA GAAGAAAAAT ATTGCAGTCA TCTACAGAAA
    CCTCGAACTC ACAAAGCTTG TAGATCTGTC AGATGCCCTT CATGGAAAGC CAATAGATGG
    AAGGAGTGCT CTGTGACCTG TGGCTCTGGA GTTCAGCAAA GAGATGTGTA CTGTAGACTC
    AGAGGCGTTG GTCGGGTGGC TGAAGAAATG TGTGCTCAAT CCACTCGGCC TTATTCTCAG
    CGGCAATGTT GGCACCAGGA CTGCATACAG TACCAGTGGG TGGCAGGAGA GTGGCTGAAC
    TGTTCAACTT CATGTAACCA AAAACAGACA CATCGGCAAG TCAAGTGCAC TGATGCACAA
    AACAGTCAAG TGAATGAGAG TTTCTGTGAT CCTTCCACCA GGCCTCTTTC CATCAAAGAG
    TGCCCGAATC CTTCCTGCAG GTACATTGTG GTAACAGGAG ACTCATCACA GTGTGCAGGT
    AACTGTGGAT TTAGTCACCT ACAAAGGATT ACATATTGCA TTGCAATCTG GCCTACTGAG
    AAATATAAGC TTCATCAACT TTGGCCTATA GACTATCGAG AATGTCCTGT GCTGCCTTCC
    CCTCAGGTTT ACAAATGCAA TTTTAGGAGC TGTTTGCATA TGGCCACTTG GAAAGTGGGG
    AAGTGGAGCA AGTGCTCAGT GACTTGCGGA GCTGGGACAA TGGAGAGAAG AGTAGAATGC
    ATGGCTGAAC ATGGCTGGCC CAGTAACTTA TGTTTAAAGC GTTTAAAACC AAATGCTCAA
    AAGAAGTGTT ACATCAATGA CTGTAAAACA TTCACCAGCT GCAAAGAAAT CCAAGTGAAA
    AACAACATTA CCAAGGATGG TGACTATGAT CTTAACATTA ACGGAAGAAT AATAAAGATC
    TATTGCGCAG GCATGCACTC AGAGAACCCC AAGGAATATA TATCATTGGT CAAAGGTGAA
    GAAGACAACT TCTCTGAAGT GTATGGTGTT AGACTACAAA ATCCATATGA ATGTCCTTTT
    AATGGAAGTA GGAGGCAAGA CTGTGCATGT AAAAATGACT ACCTGGCTGC TGGACGCACT
    GTTTTCAGCA AAATAAGAAT TGATGTCAAT TCCATGCAAA TTAAAACCAC AGATCTTCTT
    TTTGCCCAGA CAATATTTGG AAAAGCAGTG CCATTTGCCA CAGCTGGAGA TTGCTATAGT
    GCTGCCAGAT GCCCACAGGG GCAGTTCAGC ATTAACTTGG CAGGAACGGG GATGAAGATA
    TCCAGCACAG CAAAATGGCT TGCTCAGGGC AATTATGCCT CTGTCATCAT ACACAGATCA
    CAAGATGGAA CCAAAGTCTA CGGCAGATGT GGAGGGTTCT GTGGAAAGTG TGTTCCTCAC
    ATGACTACTG GTCTCCCAAT TCAAGGTGCT CATCAGGGAA TCAAATCACT GCTTAATTTT
    TCCAAAGCCT TCAAAGTAAA CTTAACAAAT ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282738
    Clone ID Related Accession (Same CDS sequence) XM_022410989.1
    Accession Version XM_022410989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGTG TTCTGTGTCT TGTGGACAAG GTAATCAAGC CCGTTATGTA
    AGCTGTCGTG ATGCTTATGA TGGAATAGCA GATGAATCTT ATTGTGCCCA TTTACCCCGA
    CCTGCTGAAA TATCTGTTTG TTTTAACCCT TGTGGGGAGT GGCAAACTGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCATGGAAAA ACAACTCGAC AAGTTTTATG CATCAACTAC
    CATCAGCCAA TTAATGAGAA TTACTGTGAC CCTGCAGTTC GCCCTTTGAT AGAACAAGAA
    TGTAACCTGG CAGCCTGCCC ACCCATGTAC AGTCACTTTC CAAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAGGATAA TCAAAATCAG
    GGGGTCTACC CGTCAATCAG AGGAAACCAA TGGAGAACAG GACCATGGGG ATCATGCTCC
    AGCAGCTGTG CTCGAGGTGT TCAGCGCAGA GTTGTGGTCT GCCAGGATGA AGACGGACAG
    AGTGCCACTT CCTGTGATGC AGCCTCCAGG CCCCCAGAGG CCCAGCACTG CCATTCAGGC
    CCTTGTCCAC AGTGGAGCTA CGGCAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCAA GATTCGTTAT ATGTCAATTT CCCAATGGCC AAATGTCACA AGAGCAAAAC
    TGTGAAATCT TAAACAAGCC ACCTAGTGTG GTACAATGTC ATGTGCATGC TTGCCCTGAT
    GATGCAACAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCTTGTGG TAAAGGTTTA
    AAGTACCGTG AGGTGCTTTG CATTGACCAG TTCCAAGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACTCACAAA GCTTGTAGAT CTGTCAGATG CCCTTCATGG
    AAAGCCAATA GATGGAAGGA GTGCTCTGTG ACCTGTGGCT CTGGAGTTCA GCAAAGAGAT
    GTGTACTGTA GACTCAGAGG CGTTGGTCGG GTGGCTGAAG AAATGTGTGC TCAATCCACT
    CGGCCTTATT CTCAGCGGCA ATGTTGGCAC CAGGACTGCA TACAGTACCA GTGGGTGGCA
    GGAGAGTGGC TGAACTGTTC AACTTCATGT AACCAAAAAC AGACACATCG GCAAGTCAAG
    TGCACTGATG CACAAAACAG TCAAGTGAAT GAGAGTTTCT GTGATCCTTC CACCAGGCCT
    CTTTCCATCA AAGAGTGCCC GAATCCTTCC TGCAGGTACA TTGTGGTAAC AGGAGACTCA
    TCACAGTGTG CAGGTAACTG TGGATTTAGT CACCTACAAA GGATTACATA TTGCATTGCA
    ATCTGGCCTA CTGAGAAATA TAAGCTTCAT CAACTTTGGC CTATAGACTA TCGAGAATGT
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGAGCTGTTT GCATATGGCC
    ACTTGGAAAG TGGGGAAGTG GAGCAAGTGC TCAGTGACTT GCGGAGCTGG GACAATGGAG
    AGAAGAGTAG AATGCATGGC TGAACATGGC TGGCCCAGTA ACTTATGTTT AAAGCGTTTA
    AAACCAAATG CTCAAAAGAA GTGTTACATC AATGACTGTA AAACATTCAC CAGCTGCAAA
    GAAATCCAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATGATCTTAA CATTAACGGA
    AGAATAATAA AGATCTATTG CGCAGGCATG CACTCAGAGA ACCCCAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTCTCT GAAGTGTATG GTGTTAGACT ACAAAATCCA
    TATGAATGTC CTTTTAATGG AAGTAGGAGG CAAGACTGTG CATGTAAAAA TGACTACCTG
    GCTGCTGGAC GCACTGTTTT CAGCAAAATA AGAATTGATG TCAATTCCAT GCAAATTAAA
    ACCACAGATC TTCTTTTTGC CCAGACAATA TTTGGAAAAG CAGTGCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGGCAGT TCAGCATTAA CTTGGCAGGA
    ACGGGGATGA AGATATCCAG CACAGCAAAA TGGCTTGCTC AGGGCAATTA TGCCTCTGTC
    ATCATACACA GATCACAAGA TGGAACCAAA GTCTACGGCA GATGTGGAGG GTTCTGTGGA
    AAGTGTGTTC CTCACATGAC TACTGGTCTC CCAATTCAAG GTGCTCATCA GGGAATCAAA
    TCACTGCTTA ATTTTTCCAA AGCCTTCAAA GTAAACTTAA CAAATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266697.1
    CDS72..5822
    Translation

    Target ORF information:

    RefSeq Version XM_022410989.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGTG TTCTGTGTCT TGTGGACAAG GTAATCAAGC CCGTTATGTA
    AGCTGTCGTG ATGCTTATGA TGGAATAGCA GATGAATCTT ATTGTGCCCA TTTACCCCGA
    CCTGCTGAAA TATCTGTTTG TTTTAACCCT TGTGGGGAGT GGCAAACTGG GAATTGGTCA
    CCTTGTTCAG CTTCCTGTGG CCATGGAAAA ACAACTCGAC AAGTTTTATG CATCAACTAC
    CATCAGCCAA TTAATGAGAA TTACTGTGAC CCTGCAGTTC GCCCTTTGAT AGAACAAGAA
    TGTAACCTGG CAGCCTGCCC ACCCATGTAC AGTCACTTTC CAAGTTCCTC TGAGCAGCCA
    AGCCATTTTC CAGGCAGGAA TTTTCCATTA ACTCACAAAC CAGAGGATAA TCAAAATCAG
    GGGGTCTACC CGTCAATCAG AGGAAACCAA TGGAGAACAG GACCATGGGG ATCATGCTCC
    AGCAGCTGTG CTCGAGGTGT TCAGCGCAGA GTTGTGGTCT GCCAGGATGA AGACGGACAG
    AGTGCCACTT CCTGTGATGC AGCCTCCAGG CCCCCAGAGG CCCAGCACTG CCATTCAGGC
    CCTTGTCCAC AGTGGAGCTA CGGCAGCTGG GGAGAATGTA CACAAACATG TGGAGGAGGA
    ATAAAATCAA GATTCGTTAT ATGTCAATTT CCCAATGGCC AAATGTCACA AGAGCAAAAC
    TGTGAAATCT TAAACAAGCC ACCTAGTGTG GTACAATGTC ATGTGCATGC TTGCCCTGAT
    GATGCAACAT GGCATCGGGG ACCATGGAAA TCGTGCTCAG CTTCTTGTGG TAAAGGTTTA
    AAGTACCGTG AGGTGCTTTG CATTGACCAG TTCCAAGGGA AATTAGAAGA AAAATATTGC
    AGTCATCTAC AGAAACCTCG AACTCACAAA GCTTGTAGAT CTGTCAGATG CCCTTCATGG
    AAAGCCAATA GATGGAAGGA GTGCTCTGTG ACCTGTGGCT CTGGAGTTCA GCAAAGAGAT
    GTGTACTGTA GACTCAGAGG CGTTGGTCGG GTGGCTGAAG AAATGTGTGC TCAATCCACT
    CGGCCTTATT CTCAGCGGCA ATGTTGGCAC CAGGACTGCA TACAGTACCA GTGGGTGGCA
    GGAGAGTGGC TGAACTGTTC AACTTCATGT AACCAAAAAC AGACACATCG GCAAGTCAAG
    TGCACTGATG CACAAAACAG TCAAGTGAAT GAGAGTTTCT GTGATCCTTC CACCAGGCCT
    CTTTCCATCA AAGAGTGCCC GAATCCTTCC TGCAGGTACA TTGTGGTAAC AGGAGACTCA
    TCACAGTGTG CAGGTAACTG TGGATTTAGT CACCTACAAA GGATTACATA TTGCATTGCA
    ATCTGGCCTA CTGAGAAATA TAAGCTTCAT CAACTTTGGC CTATAGACTA TCGAGAATGT
    CCTGTGCTGC CTTCCCCTCA GGTTTACAAA TGCAATTTTA GGAGCTGTTT GCATATGGCC
    ACTTGGAAAG TGGGGAAGTG GAGCAAGTGC TCAGTGACTT GCGGAGCTGG GACAATGGAG
    AGAAGAGTAG AATGCATGGC TGAACATGGC TGGCCCAGTA ACTTATGTTT AAAGCGTTTA
    AAACCAAATG CTCAAAAGAA GTGTTACATC AATGACTGTA AAACATTCAC CAGCTGCAAA
    GAAATCCAAG TGAAAAACAA CATTACCAAG GATGGTGACT ATGATCTTAA CATTAACGGA
    AGAATAATAA AGATCTATTG CGCAGGCATG CACTCAGAGA ACCCCAAGGA ATATATATCA
    TTGGTCAAAG GTGAAGAAGA CAACTTCTCT GAAGTGTATG GTGTTAGACT ACAAAATCCA
    TATGAATGTC CTTTTAATGG AAGTAGGAGG CAAGACTGTG CATGTAAAAA TGACTACCTG
    GCTGCTGGAC GCACTGTTTT CAGCAAAATA AGAATTGATG TCAATTCCAT GCAAATTAAA
    ACCACAGATC TTCTTTTTGC CCAGACAATA TTTGGAAAAG CAGTGCCATT TGCCACAGCT
    GGAGATTGCT ATAGTGCTGC CAGATGCCCA CAGGGGCAGT TCAGCATTAA CTTGGCAGGA
    ACGGGGATGA AGATATCCAG CACAGCAAAA TGGCTTGCTC AGGGCAATTA TGCCTCTGTC
    ATCATACACA GATCACAAGA TGGAACCAAA GTCTACGGCA GATGTGGAGG GTTCTGTGGA
    AAGTGTGTTC CTCACATGAC TACTGGTCTC CCAATTCAAG GTGCTCATCA GGGAATCAAA
    TCACTGCTTA ATTTTTCCAA AGCCTTCAAA GTAAACTTAA CAAATACATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282739
    Clone ID Related Accession (Same CDS sequence) XM_022410990.1
    Accession Version XM_022410990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGGA CTGTATAGTC ATACCTTGCC CAATTATCCC TAAAATTGGG
    GCCACTTCAT TGCCAGCTGT TCCCATAGGA AAGATAGCCC AGTGGCGACA TGGTTCTTGG
    ACCCCATGTT CTGTGTCTTG TGGACAAGGT AATCAAGCCC GTTATGTAAG CTGTCGTGAT
    GCTTATGATG GAATAGCAGA TGAATCTTAT TGTGCCCATT TACCCCGACC TGCTGAAATA
    TCTGTTTGTT TTAACCCTTG TGGGGAGTGG CAAACTGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC ATGGAAAAAC AACTCGACAA GTTTTATGCA TCAACTACCA TCAGCCAATT
    AATGAGAATT ACTGTGACCC TGCAGTTCGC CCTTTGATAG AACAAGAATG TAACCTGGCA
    GCCTGCCCAC CCATGTACAG TCACTTTCCA AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAGGATAATC AAAATCAGGG GGTCTACCCG
    TCAATCAGAG GAAACCAATG GAGAACAGGA CCATGGGGAT CATGCTCCAG CAGCTGTGCT
    CGAGGTGTTC AGCGCAGAGT TGTGGTCTGC CAGGATGAAG ACGGACAGAG TGCCACTTCC
    TGTGATGCAG CCTCCAGGCC CCCAGAGGCC CAGCACTGCC ATTCAGGCCC TTGTCCACAG
    TGGAGCTACG GCAGCTGGGG AGAATGCTCA GCTTCTTGTG GTAAAGGTTT AAAGTACCGT
    GAGGTGCTTT GCATTGACCA GTTCCAAGGG AAATTAGAAG AAAAATATTG CAGTCATCTA
    CAGAAACCTC GAACTCACAA AGCTTGTAGA TCTGTCAGAT GCCCTTCATG GAAAGCCAAT
    AGATGGAAGG AGTGCTCTGT GACCTGTGGC TCTGGAGTTC AGCAAAGAGA TGTGTACTGT
    AGACTCAGAG GCGTTGGTCG GGTGGCTGAA GAAATGTGTG CTCAATCCAC TCGGCCTTAT
    TCTCAGCGGC AATGTTGGCA CCAGGACTGC ATACAGTACC AGTGGGTGGC AGGAGAGTGG
    CTGAACTGTT CAACTTCATG TAACCAAAAA CAGACACATC GGCAAGTCAA GTGCACTGAT
    GCACAAAACA GTCAAGTGAA TGAGAGTTTC TGTGATCCTT CCACCAGGCC TCTTTCCATC
    AAAGAGTGCC CGAATCCTTC CTGCAGGTAC ATTGTGGTAA CAGGAGACTC ATCACAGTGT
    GCAGGTAACT GTGGATTTAG TCACCTACAA AGGATTACAT ATTGCATTGC AATCTGGCCT
    ACTGAGAAAT ATAAGCTTCA TCAACTTTGG CCTATAGACT ATCGAGAATG TCCTGTGCTG
    CCTTCCCCTC AGGTTTACAA ATGCAATTTT AGGAGCTGTT TGCATATGGC CACTTGGAAA
    GTGGGGAAGT GGAGCAAGTG CTCAGTGACT TGCGGAGCTG GGACAATGGA GAGAAGAGTA
    GAATGCATGG CTGAACATGG CTGGCCCAGT AACTTATGTT TAAAGCGTTT AAAACCAAAT
    GCTCAAAAGA AGTGTTACAT CAATGACTGT AAAACATTCA CCAGCTGCAA AGAAATCCAA
    GTGAAAAACA ACATTACCAA GGATGGTGAC TATGATCTTA ACATTAACGG AAGAATAATA
    AAGATCTATT GCGCAGGCAT GCACTCAGAG AACCCCAAGG AATATATATC ATTGGTCAAA
    GGTGAAGAAG ACAACTTCTC TGAAGTGTAT GGTGTTAGAC TACAAAATCC ATATGAATGT
    CCTTTTAATG GAAGTAGGAG GCAAGACTGT GCATGTAAAA ATGACTACCT GGCTGCTGGA
    CGCACTGTTT TCAGCAAAAT AAGAATTGAT GTCAATTCCA TGCAAATTAA AACCACAGAT
    CTTCTTTTTG CCCAGACAAT ATTTGGAAAA GCAGTGCCAT TTGCCACAGC TGGAGATTGC
    TATAGTGCTG CCAGATGCCC ACAGGGGCAG TTCAGCATTA ACTTGGCAGG AACGGGGATG
    AAGATATCCA GCACAGCAAA ATGGCTTGCT CAGGGCAATT ATGCCTCTGT CATCATACAC
    AGATCACAAG ATGGAACCAA AGTCTACGGC AGATGTGGAG GGTTCTGTGG AAAGTGTGTT
    CCTCACATGA CTACTGGTCT CCCAATTCAA GGTGCTCATC AGGGAATCAA ATCACTGCTT
    AATTTTTCCA AAGCCTTCAA AGTAAACTTA ACAAATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266698.1
    CDS72..5753
    Translation

    Target ORF information:

    RefSeq Version XM_022410990.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGGA CTGTATAGTC ATACCTTGCC CAATTATCCC TAAAATTGGG
    GCCACTTCAT TGCCAGCTGT TCCCATAGGA AAGATAGCCC AGTGGCGACA TGGTTCTTGG
    ACCCCATGTT CTGTGTCTTG TGGACAAGGT AATCAAGCCC GTTATGTAAG CTGTCGTGAT
    GCTTATGATG GAATAGCAGA TGAATCTTAT TGTGCCCATT TACCCCGACC TGCTGAAATA
    TCTGTTTGTT TTAACCCTTG TGGGGAGTGG CAAACTGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC ATGGAAAAAC AACTCGACAA GTTTTATGCA TCAACTACCA TCAGCCAATT
    AATGAGAATT ACTGTGACCC TGCAGTTCGC CCTTTGATAG AACAAGAATG TAACCTGGCA
    GCCTGCCCAC CCATGTACAG TCACTTTCCA AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAGGATAATC AAAATCAGGG GGTCTACCCG
    TCAATCAGAG GAAACCAATG GAGAACAGGA CCATGGGGAT CATGCTCCAG CAGCTGTGCT
    CGAGGTGTTC AGCGCAGAGT TGTGGTCTGC CAGGATGAAG ACGGACAGAG TGCCACTTCC
    TGTGATGCAG CCTCCAGGCC CCCAGAGGCC CAGCACTGCC ATTCAGGCCC TTGTCCACAG
    TGGAGCTACG GCAGCTGGGG AGAATGCTCA GCTTCTTGTG GTAAAGGTTT AAAGTACCGT
    GAGGTGCTTT GCATTGACCA GTTCCAAGGG AAATTAGAAG AAAAATATTG CAGTCATCTA
    CAGAAACCTC GAACTCACAA AGCTTGTAGA TCTGTCAGAT GCCCTTCATG GAAAGCCAAT
    AGATGGAAGG AGTGCTCTGT GACCTGTGGC TCTGGAGTTC AGCAAAGAGA TGTGTACTGT
    AGACTCAGAG GCGTTGGTCG GGTGGCTGAA GAAATGTGTG CTCAATCCAC TCGGCCTTAT
    TCTCAGCGGC AATGTTGGCA CCAGGACTGC ATACAGTACC AGTGGGTGGC AGGAGAGTGG
    CTGAACTGTT CAACTTCATG TAACCAAAAA CAGACACATC GGCAAGTCAA GTGCACTGAT
    GCACAAAACA GTCAAGTGAA TGAGAGTTTC TGTGATCCTT CCACCAGGCC TCTTTCCATC
    AAAGAGTGCC CGAATCCTTC CTGCAGGTAC ATTGTGGTAA CAGGAGACTC ATCACAGTGT
    GCAGGTAACT GTGGATTTAG TCACCTACAA AGGATTACAT ATTGCATTGC AATCTGGCCT
    ACTGAGAAAT ATAAGCTTCA TCAACTTTGG CCTATAGACT ATCGAGAATG TCCTGTGCTG
    CCTTCCCCTC AGGTTTACAA ATGCAATTTT AGGAGCTGTT TGCATATGGC CACTTGGAAA
    GTGGGGAAGT GGAGCAAGTG CTCAGTGACT TGCGGAGCTG GGACAATGGA GAGAAGAGTA
    GAATGCATGG CTGAACATGG CTGGCCCAGT AACTTATGTT TAAAGCGTTT AAAACCAAAT
    GCTCAAAAGA AGTGTTACAT CAATGACTGT AAAACATTCA CCAGCTGCAA AGAAATCCAA
    GTGAAAAACA ACATTACCAA GGATGGTGAC TATGATCTTA ACATTAACGG AAGAATAATA
    AAGATCTATT GCGCAGGCAT GCACTCAGAG AACCCCAAGG AATATATATC ATTGGTCAAA
    GGTGAAGAAG ACAACTTCTC TGAAGTGTAT GGTGTTAGAC TACAAAATCC ATATGAATGT
    CCTTTTAATG GAAGTAGGAG GCAAGACTGT GCATGTAAAA ATGACTACCT GGCTGCTGGA
    CGCACTGTTT TCAGCAAAAT AAGAATTGAT GTCAATTCCA TGCAAATTAA AACCACAGAT
    CTTCTTTTTG CCCAGACAAT ATTTGGAAAA GCAGTGCCAT TTGCCACAGC TGGAGATTGC
    TATAGTGCTG CCAGATGCCC ACAGGGGCAG TTCAGCATTA ACTTGGCAGG AACGGGGATG
    AAGATATCCA GCACAGCAAA ATGGCTTGCT CAGGGCAATT ATGCCTCTGT CATCATACAC
    AGATCACAAG ATGGAACCAA AGTCTACGGC AGATGTGGAG GGTTCTGTGG AAAGTGTGTT
    CCTCACATGA CTACTGGTCT CCCAATTCAA GGTGCTCATC AGGGAATCAA ATCACTGCTT
    AATTTTTCCA AAGCCTTCAA AGTAAACTTA ACAAATACAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe282740
    Clone ID Related Accession (Same CDS sequence) XM_022410991.1
    Accession Version XM_022410991.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5658bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product A disintegrin and metalloproteinase with thrombospondin motifs 20 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006609.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGGA CTGTATAGTC ATACCTTGCC CAATTATCCC TAAAATTGGG
    GCCACTTCAT TGCCAGCTGT TCCCATAGGA AAGATAGCCC AGTGGCGACA TGGTTCTTGG
    ACCCCATGTT CTGTGTCTTG TGGACAAGGT AATCAAGCCC GTTATGTAAG CTGTCGTGAT
    GCTTATGATG GAATAGCAGA TGAATCTTAT TGTGCCCATT TACCCCGACC TGCTGAAATA
    TCTGTTTGTT TTAACCCTTG TGGGGAGTGG CAAACTGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC ATGGAAAAAC AACTCGACAA GTTTTATGCA TCAACTACCA TCAGCCAATT
    AATGAGAATT ACTGTGACCC TGCAGTTCGC CCTTTGATAG AACAAGAATG TAACCTGGCA
    GCCTGCCCAC CCATGTACAG TCACTTTCCA AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAGGATAATC AAAATCAGGG GGTCTACCCG
    TCAATCAGAG GAAACCAATG GAGAACAGGA CCATGGGGAT CATGCTCCAG CAGCTGTGCT
    CGAGGTGTTC AGCGCAGAGT TGTGGTCTGC CAGGATGAAG ACGGACAGAG TGCCACTTCC
    TGTGATGCAG CCTCCAGGCC CCCAGAGGCC CAGCACTGCC ATTCAGGCCC TTGTCCACAG
    TGGAGCTACG GCAGCTGGGG AGAATGTACA CAAACATGTG GAGGAGGAAT AAAATCAAGA
    TTCGTTATAT GTCAATTTCC CAATGGCCAA ATGTCACAAG AGCAAAACTG TGAAATCTTA
    AACAAGCCAC CTAGTGTGGT ACAATGTCAT GTGCATGCTT GCCCTGATGA TGCAACATGG
    CATCGGGGAC CATGGAAATC GTGCTCAGCT TCTTGTGGTA AAGGTTTAAA GTACCGTGAG
    GTGCTTTGCA TTGACCAGTT CCAAGGGAAA TTAGAAGAAA AATATTGCAG TCATCTACAG
    AAACCTCGAA CTCACAAAGC TTGTAGATCT GTCAGATGCC CTTCATGGAA AGCCAATAGA
    TGGAAGGAGT GCTCTGTGAC CTGTGGCTCT GGAGTTCAGC AAAGAGATGT GTACTGTAGA
    CTCAGAGGCG TTGGTCGGGT GGCTGAAGAA ATGTGTGCTC AATCCACTCG GCCTTATTCT
    CAGCGGCAAT GTTGGCACCA GGACTGCATA CAGTACCAGT GGGTGGCAGG AGAGTGGCTG
    AACTGTTCAA CTTCATGTAA CCAAAAACAG ACACATCGGC AAGTCAAGTG CACTGATGCA
    CAAAACAGTC AAGTGAATGA GAGTTTCTGT GATCCTTCCA CCAGGCCTCT TTCCATCAAA
    GAGTGCCCGA ATCCTTCCTG CAGGTACATT GTGGTAACAG GAGACTCATC ACAGTGCTCA
    GTGACTTGCG GAGCTGGGAC AATGGAGAGA AGAGTAGAAT GCATGGCTGA ACATGGCTGG
    CCCAGTAACT TATGTTTAAA GCGTTTAAAA CCAAATGCTC AAAAGAAGTG TTACATCAAT
    GACTGTAAAA CATTCACCAG CTGCAAAGAA ATCCAAGTGA AAAACAACAT TACCAAGGAT
    GGTGACTATG ATCTTAACAT TAACGGAAGA ATAATAAAGA TCTATTGCGC AGGCATGCAC
    TCAGAGAACC CCAAGGAATA TATATCATTG GTCAAAGGTG AAGAAGACAA CTTCTCTGAA
    GTGTATGGTG TTAGACTACA AAATCCATAT GAATGTCCTT TTAATGGAAG TAGGAGGCAA
    GACTGTGCAT GTAAAAATGA CTACCTGGCT GCTGGACGCA CTGTTTTCAG CAAAATAAGA
    ATTGATGTCA ATTCCATGCA AATTAAAACC ACAGATCTTC TTTTTGCCCA GACAATATTT
    GGAAAAGCAG TGCCATTTGC CACAGCTGGA GATTGCTATA GTGCTGCCAG ATGCCCACAG
    GGGCAGTTCA GCATTAACTT GGCAGGAACG GGGATGAAGA TATCCAGCAC AGCAAAATGG
    CTTGCTCAGG GCAATTATGC CTCTGTCATC ATACACAGAT CACAAGATGG AACCAAAGTC
    TACGGCAGAT GTGGAGGGTT CTGTGGAAAG TGTGTTCCTC ACATGACTAC TGGTCTCCCA
    ATTCAAGGTG CTCATCAGGG AATCAAATCA CTGCTTAATT TTTCCAAAGC CTTCAAAGTA
    AACTTAACAA ATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022266699.1
    CDS72..5729
    Translation

    Target ORF information:

    RefSeq Version XM_022410991.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ADAM metallopeptidase with thrombospondin type 1 motif 20 (ADAMTS20), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410991.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCGGGTGG CCAAGTGGCT GACGGGGCTG CTCTATCAGC TCTCGCTCTT CATCGCCAGG 
    TCTTGGGAAA TTCACTTGCA CCCCAGGCAA GAAGCCCTGG TGAGGACGCT GGTCTCCTAC
    GAAGTGGTGA CCCCCGCGCG GGTCAACGAG TTTGGCGAGG TGTTCCCGCA GAGCCACCAC
    TTCAGCCGGA GGAAGCGCAG CTCCGAGGCG CCGGGGCCGC CGCCGGCCAG AACGCACTAC
    CGGATCAGCG CATACGGGCA GCTCTTCAAA AAGAACCTGT GCGCGGACGC GGCCTTCCTG
    GGCGCCGGCT ACACCGAGGT GCACCTGGGC GCCCCCGCGC GCGGGGCGGA GGGGCACGGC
    GGGGCGCCCC CGGACCTGCG CCACTGCTTC TACCGCGGCC AGGTCAACGC TCGCCGGGAG
    CACACGGCGG TCTTCAGCCT CTGCGGAGGC CTGATGGGAA CATTTAAAGC ACATGATGGT
    GAATATTTCT TAGAACCTAT AATGAAGGCA GATGGGAGCG AGCATGAAGA TGATCATAAC
    AAACCACATC TTATATACAG ACAGGAAATA AAGAGCAACT ATTTTCTGCA ATCTCACAAG
    CCTTGTGAAG TTTCAGAAAG TCAAATAAAG CAAACCACTT TACCCCTTCA CAACTACAGC
    AACATGAACG AAGATCCTAA CGTAAAGAAG GAAATGGTTT TAGGGCACCC TTCAAAAAAT
    AAATCCTTGG AAGATGAAAG AAGTCAATTA CATTCCAGGA AAAAACGTTT CTTATCTTAT
    CCAAGATACG TGGAAGTCAT GGTTACAGCT GATGCTAAAA TGGTTCATCA TCATGGACAG
    AATCTGCAGC ACTATGTACT AACTCTAATG TCAATTGTTG CAGCAATCTA CAAGGACTCA
    AGTATTGGAA ATCTCATCAA CATAGTAATT GTAAAATTAG TTGTAATTCA TAATGAACAG
    GAAGGACCAG TCATCAGTTA CAATGCAGCT ACCACACTGC GCAACTTCTG TTTGTGGCAG
    CAAACTCAGA ATGTTCTTGA TGATGCTCAC CCTTCTCACC ATGACACTGC TGTTCTTATC
    ACTAGGGAAG ACATTTGTGG AGCTAGAGAG AAATGTGACA CATTGGGTTT AGCAGAGTTA
    GGTACCCTAT GTGATCCTTT ACGGAGCTGC TCTATTAGTG AAGAAAATGG ACTCAGCGCT
    GCCTTTACCA TCGCCCACGA GCTCGGGCAT GTATTTAATG TTCCACATGA TGATAGTTTT
    AAATGTAAGG AAACTGGAAG TAAACATCAA TATCATGTAA TGGCTCCAAC TTTAAATTAC
    CACACAAGTC CTTGGACCTG GTCAAAATGT AGTCAGAAAT ATATCACTGA ATTCCTAGAT
    ACTGGTCATG GGGAATGCCT CCTTGACAGA CCAAATGGAA GAATTTATGA TCTATCTTCA
    CAACCTCCTG GATTAATGTA TGATGTAAAC AAGCAGTGTG AACTTATGTT TGGTCCTGGA
    TCACAAGTGT GTCCATACTT GAAGCAGTGC AGGCGTCTCT GGTGCACTAG TGCAGAAGGA
    GTACACAAGG GCTGTCGCAC TCAACACATG CCCCTGGCAG ATGGAACACA CTGTGGTCCT
    GGGATGCATT GCCATCATGG GCTATGTGTA AACAAAGAAA TGGAAACACG TCCGGTAGAT
    GGTGAATGGG GACCGTGGGG ACCTTACAGC TCTTGTTCAA GAACATGTGG AGGTGGAATC
    AAAAGCACAA CCAGGCTCTG TAATCGCCCT GAGCCAAGAA ACGGAGGAAA GTACTGTGTG
    GGCCGCAGGA TGAAATTTCG ATCATGTAAT ACTGATTCAT GTCCAAAAGG CAAGCAAGAC
    TTTCGAGAGA AGCAGTGCTC TGATTTTGAT GGTAAACATT TCAACATCAA TGGTCTTTCC
    CCTAATGTGA GGTGGCTTCC AAAATACAGT GGGATTGCCA TAAAAGATCG CTGTAAACTG
    TATTGTCGGG TTGCCGGAAC CACTAACTTC TACCAGTTGA AGGATAGGGT TGCTGATGGT
    ACTCCTTGTG GAACCGAAAC TAATGATATC TGTGTTCAAG GCCTCTGTCG GCAAGCTGGC
    TGTGATCATG TGTTAAACTC CAAGGCCAGG AGAGACAAAT GTGGAGTGTG TGGTGGGGAT
    AACTCTTCAT GCAGGACAAT GACAGGTGTC TTCAACAGTG CTCATTATGG TTATAATGTT
    GTTGTCAAGA TTCCTGCAGG AGCAACAAAC ATTGATATTC TTCAGCATAG CTATTCTGGA
    AAACCAGAAG ATGACAATTA CCTTGCATTA TCTGACACTC AAGGGAATTT TCTTTTGAAT
    GGGAATTTTG TTGTAAGTAT GTCAAAAAAA GAAATCAACA TACAAGGAGC TATTTTTGAA
    TACAGTGGTT CAAACAGCTC AATTGAAAGA ATTAATAGTA CTGATCGGCT GGAAGAAGAA
    GTTGTTTTGC AGGTTTTATG TGTGGGTAAT TTATACAACC CTGATGTACG ATATTCCTTC
    AATATCCCCG TGGAAGAGAA GAACGACCTA GTCACATGGG ATCCGTATGG ACCATGGCAA
    GACTGCACCA AGATGTGTCA AGGTACACTG GTGTTGAGTG AAAGAGACAG AAGCTTGCCT
    GTGCATGCTG GTCTTCACAG AAGAAAAATT ACCTGCATTC GGAAGAGTGA TCACATGGTA
    GTGTCCGATC AAAGATGTGA CCATTTGCCA CTTCCATTGT TTGTGACTGA AAGGTGTAAT
    ACAGACTGTG AACTAAGGTG GCACATCATT AGCAAAAGTG AATGTTCATC CCATTGTGGT
    CAAGGATATA GATCACTGGA TGTCCACTGC ATGAAGTACT CCATTCATAA AGGACAGACT
    GTTCCAGTAG ATGACCACTA CTGTGGTGAC CAACTTAAAC CTCCCACACG AGAACCCTGC
    CATGGTGACT GTGTCTTAAC AAGATGGCAT TATTCAGAAT GGTCCCAGTG TTCCAGGAGT
    TGTGGAGGAG GGGAAAGGTC TCGAGAATCG TACTGTGTAA ATAACTTTGG CCACCATGTT
    GCCAGCAGAG AATGCCAAGA GCTTCCCCGA GTGACAACGG AGAATTGCAA TGAATTTTCC
    TGTCCCAGTT GGGCTACTAG TGAGTGGAGT GAGTGTCTTG TTACATGTGG AAAAGGAACA
    AAACAAAGGC AAGTGTGGTG CCAGCTGAAT GAAGATCATT TGAATGATGG TCTCTGTAAC
    CCGGGTACCA AACCTGAATC TTTGAGACCA TGTGAGCTTC ATACATGTGC TTCCTGGCAA
    GTAGGACCGT GGGGTTCTTG CACGGCCACA TGTGGACGTG GGTATCAGAT GCGTGCTGTT
    AAATGTGTCA ATGAGCTGCT CAGTGCAGTG TTAGATGACA GAGTGTGCCA TGGAGCCAGT
    CGCCCAAGTG ACAGGCAGGA CTGTATAGTC ATACCTTGCC CAATTATCCC TAAAATTGGG
    GCCACTTCAT TGCCAGCTGT TCCCATAGGA AAGATAGCCC AGTGGCGACA TGGTTCTTGG
    ACCCCATGTT CTGTGTCTTG TGGACAAGGT AATCAAGCCC GTTATGTAAG CTGTCGTGAT
    GCTTATGATG GAATAGCAGA TGAATCTTAT TGTGCCCATT TACCCCGACC TGCTGAAATA
    TCTGTTTGTT TTAACCCTTG TGGGGAGTGG CAAACTGGGA ATTGGTCACC TTGTTCAGCT
    TCCTGTGGCC ATGGAAAAAC AACTCGACAA GTTTTATGCA TCAACTACCA TCAGCCAATT
    AATGAGAATT ACTGTGACCC TGCAGTTCGC CCTTTGATAG AACAAGAATG TAACCTGGCA
    GCCTGCCCAC CCATGTACAG TCACTTTCCA AGTTCCTCTG AGCAGCCAAG CCATTTTCCA
    GGCAGGAATT TTCCATTAAC TCACAAACCA GAGGATAATC AAAATCAGGG GGTCTACCCG
    TCAATCAGAG GAAACCAATG GAGAACAGGA CCATGGGGAT CATGCTCCAG CAGCTGTGCT
    CGAGGTGTTC AGCGCAGAGT TGTGGTCTGC CAGGATGAAG ACGGACAGAG TGCCACTTCC
    TGTGATGCAG CCTCCAGGCC CCCAGAGGCC CAGCACTGCC ATTCAGGCCC TTGTCCACAG
    TGGAGCTACG GCAGCTGGGG AGAATGTACA CAAACATGTG GAGGAGGAAT AAAATCAAGA
    TTCGTTATAT GTCAATTTCC CAATGGCCAA ATGTCACAAG AGCAAAACTG TGAAATCTTA
    AACAAGCCAC CTAGTGTGGT ACAATGTCAT GTGCATGCTT GCCCTGATGA TGCAACATGG
    CATCGGGGAC CATGGAAATC GTGCTCAGCT TCTTGTGGTA AAGGTTTAAA GTACCGTGAG
    GTGCTTTGCA TTGACCAGTT CCAAGGGAAA TTAGAAGAAA AATATTGCAG TCATCTACAG
    AAACCTCGAA CTCACAAAGC TTGTAGATCT GTCAGATGCC CTTCATGGAA AGCCAATAGA
    TGGAAGGAGT GCTCTGTGAC CTGTGGCTCT GGAGTTCAGC AAAGAGATGT GTACTGTAGA
    CTCAGAGGCG TTGGTCGGGT GGCTGAAGAA ATGTGTGCTC AATCCACTCG GCCTTATTCT
    CAGCGGCAAT GTTGGCACCA GGACTGCATA CAGTACCAGT GGGTGGCAGG AGAGTGGCTG
    AACTGTTCAA CTTCATGTAA CCAAAAACAG ACACATCGGC AAGTCAAGTG CACTGATGCA
    CAAAACAGTC AAGTGAATGA GAGTTTCTGT GATCCTTCCA CCAGGCCTCT TTCCATCAAA
    GAGTGCCCGA ATCCTTCCTG CAGGTACATT GTGGTAACAG GAGACTCATC ACAGTGCTCA
    GTGACTTGCG GAGCTGGGAC AATGGAGAGA AGAGTAGAAT GCATGGCTGA ACATGGCTGG
    CCCAGTAACT TATGTTTAAA GCGTTTAAAA CCAAATGCTC AAAAGAAGTG TTACATCAAT
    GACTGTAAAA CATTCACCAG CTGCAAAGAA ATCCAAGTGA AAAACAACAT TACCAAGGAT
    GGTGACTATG ATCTTAACAT TAACGGAAGA ATAATAAAGA TCTATTGCGC AGGCATGCAC
    TCAGAGAACC CCAAGGAATA TATATCATTG GTCAAAGGTG AAGAAGACAA CTTCTCTGAA
    GTGTATGGTG TTAGACTACA AAATCCATAT GAATGTCCTT TTAATGGAAG TAGGAGGCAA
    GACTGTGCAT GTAAAAATGA CTACCTGGCT GCTGGACGCA CTGTTTTCAG CAAAATAAGA
    ATTGATGTCA ATTCCATGCA AATTAAAACC ACAGATCTTC TTTTTGCCCA GACAATATTT
    GGAAAAGCAG TGCCATTTGC CACAGCTGGA GATTGCTATA GTGCTGCCAG ATGCCCACAG
    GGGCAGTTCA GCATTAACTT GGCAGGAACG GGGATGAAGA TATCCAGCAC AGCAAAATGG
    CTTGCTCAGG GCAATTATGC CTCTGTCATC ATACACAGAT CACAAGATGG AACCAAAGTC
    TACGGCAGAT GTGGAGGGTT CTGTGGAAAG TGTGTTCCTC ACATGACTAC TGGTCTCCCA
    ATTCAAGGTG CTCATCAGGG AATCAAATCA CTGCTTAATT TTTCCAAAGC CTTCAAAGTA
    AACTTAACAA ATACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.