FREM2 cDNA ORF clone, Canis lupus familiaris(dog)

The following FREM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OCe25340 XM_543127.6
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Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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OCe25340 XM_543127.5 Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe25340
    Clone ID Related Accession (Same CDS sequence) XM_543127.5 , XM_543127.6
    Accession Version XM_543127.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_543127.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
    CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC
    GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT
    GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC
    GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC
    CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC
    CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT
    CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC
    GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT
    CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG
    AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC
    TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC
    GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC
    GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG
    GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC
    CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG
    GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG
    GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC
    CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC
    ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC
    CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC
    CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG
    AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT
    GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG
    GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC
    CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC
    AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC
    CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC
    AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT
    CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC
    TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG
    TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG
    CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT
    GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG
    TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG
    GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG
    AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC
    CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA
    ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA
    ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG
    TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG
    CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC
    CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC
    ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT
    TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA
    CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC
    GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG
    AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA
    ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC
    CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG
    TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA
    GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG
    GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT
    AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT
    GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA
    TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC
    TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG
    AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT
    ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC
    ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC
    ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT
    GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA
    AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT
    ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT
    GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC
    CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC
    AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT
    CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT
    ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC
    CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC
    GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT
    GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC
    CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT
    CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT
    CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG
    ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT
    ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC
    AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT
    CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC
    AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC
    TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT
    TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG
    ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG
    GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA
    TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT
    CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA
    CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA
    AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT
    TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC
    TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA
    AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG
    AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC
    GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT
    GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT
    GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC
    ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA
    ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG
    GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC
    GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG
    CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC
    AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC
    GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC
    ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA
    ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC
    CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT
    ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT
    GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT
    GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA
    TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT
    GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG
    ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT
    AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA
    GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC
    AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA
    AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT
    CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT
    GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC
    GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG
    GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT
    TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT
    TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC
    AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG
    ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC
    TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT
    GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA
    GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG
    CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG
    CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC
    ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC
    ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT
    GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC
    TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA
    GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG
    CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT
    GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT
    GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT
    TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG
    ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA
    ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA
    CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC
    TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC
    CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC
    CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG
    TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT
    GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG
    GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT
    GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC
    TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA
    AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT
    CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC
    CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC
    ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG
    GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA
    ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC
    CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC
    CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA
    ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA
    AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC
    TGTGACAGCT CAGAGGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543127.3
    CDS186..9686
    Translation

    Target ORF information:

    RefSeq Version XM_543127.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543127.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
    CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC
    GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT
    GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC
    GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC
    CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC
    CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT
    CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC
    GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT
    CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG
    AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC
    TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC
    GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC
    GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG
    GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC
    CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG
    GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG
    GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC
    CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC
    ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC
    CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC
    CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG
    AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT
    GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG
    GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC
    CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC
    AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC
    CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC
    AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT
    CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC
    TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG
    TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG
    CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT
    GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG
    TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG
    GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG
    AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC
    CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA
    ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA
    ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG
    TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG
    CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC
    CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC
    ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT
    TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA
    CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC
    GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG
    AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA
    ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC
    CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG
    TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA
    GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG
    GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT
    AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT
    GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA
    TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC
    TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG
    AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT
    ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC
    ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC
    ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT
    GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA
    AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT
    ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT
    GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC
    CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC
    AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT
    CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT
    ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC
    CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC
    GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT
    GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC
    CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT
    CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT
    CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG
    ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT
    ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC
    AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT
    CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC
    AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC
    TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT
    TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG
    ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG
    GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA
    TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT
    CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA
    CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA
    AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT
    TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC
    TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA
    AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG
    AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC
    GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT
    GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT
    GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC
    ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA
    ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG
    GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC
    GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG
    CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC
    AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC
    GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC
    ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA
    ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC
    CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT
    ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT
    GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT
    GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA
    TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT
    GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG
    ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT
    AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA
    GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC
    AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA
    AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT
    CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT
    GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC
    GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG
    GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT
    TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT
    TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC
    AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG
    ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC
    TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT
    GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA
    GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG
    CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG
    CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC
    ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC
    ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT
    GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC
    TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA
    GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG
    CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT
    GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT
    GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT
    TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG
    ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA
    ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA
    CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC
    TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC
    CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC
    CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG
    TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT
    GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG
    GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT
    GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC
    TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA
    AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT
    CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC
    CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC
    ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG
    GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA
    ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC
    CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC
    CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA
    ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA
    AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC
    TGTGACAGCT CAGAGGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25340
    Clone ID Related Accession (Same CDS sequence) XM_543127.5 , XM_543127.6
    Accession Version XM_543127.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product FRAS1-related extracellular matrix protein 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_543127.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
    2221
    2281
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    2401
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    2581
    2641
    2701
    2761
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    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4681
    4741
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    4981
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    6301
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    6421
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    7141
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    7381
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    7501
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    7681
    7741
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    7921
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    8401
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    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
    CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC
    GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT
    GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC
    GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC
    CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC
    CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT
    CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC
    GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT
    CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG
    AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC
    TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC
    GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC
    GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG
    GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC
    CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG
    GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG
    GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC
    CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC
    ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC
    CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC
    CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG
    AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT
    GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG
    GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC
    CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC
    AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC
    CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC
    AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT
    CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC
    TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG
    TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG
    CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT
    GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG
    TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG
    GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG
    AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC
    CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA
    ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA
    ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG
    TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG
    CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC
    CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC
    ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT
    TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA
    CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC
    GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG
    AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA
    ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC
    CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG
    TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA
    GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG
    GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT
    AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT
    GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA
    TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC
    TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG
    AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT
    ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC
    ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC
    ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT
    GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA
    AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT
    ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT
    GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC
    CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC
    AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT
    CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT
    ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC
    CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC
    GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT
    GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC
    CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT
    CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT
    CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG
    ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT
    ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC
    AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT
    CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC
    AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC
    TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT
    TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG
    ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG
    GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA
    TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT
    CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA
    CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA
    AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT
    TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC
    TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA
    AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG
    AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC
    GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT
    GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT
    GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC
    ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA
    ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG
    GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC
    GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG
    CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC
    AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC
    GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC
    ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA
    ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC
    CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT
    ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT
    GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT
    GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA
    TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT
    GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG
    ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT
    AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA
    GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC
    AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA
    AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT
    CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT
    GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC
    GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG
    GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT
    TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT
    TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC
    AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG
    ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC
    TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT
    GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA
    GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG
    CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG
    CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC
    ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC
    ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT
    GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC
    TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA
    GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG
    CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT
    GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT
    GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT
    TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG
    ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA
    ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA
    CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC
    TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC
    CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC
    CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG
    TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT
    GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG
    GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT
    GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC
    TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA
    AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT
    CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC
    CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC
    ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG
    GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA
    ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC
    CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC
    CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA
    ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA
    AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC
    TGTGACAGCT CAGAGGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_543127.3
    CDS102..9602
    Translation

    Target ORF information:

    RefSeq Version XM_543127.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix protein 2 (FREM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_543127.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    ATGCGCGCGG CCGGGGCCCC CGGGATCGCG TCCCGCGCCG CCACCGGTGC TCAGCCGGTG 
    CCCCGGCTGC CGCCTCCCCT GCTGTTGCTA CTGCCGCTTC TCCTGTCCCC GGTAAGCCGC
    GCCCGGGCAC AGCCCGCTGC CCCGGGCCGG GAGCTGCTGT CCCCGGATCC CGCGGGCGCT
    GCGGGGGTCC TCGCCGAGGA GGCCGTGGTG CTGGCGAACC GGGGGCTCCG GGTGCCCTTC
    GGCCGCGAGG TCTGGCTGGA TCCGCGGCGC GACCTGGTGC TGCAGGTGCA GCCGGGGGAC
    CGCTGCGCGG TGACCGTGCT GGACAGTGAC GCGCTGGCGC AGAGGCCGGG CCGCCTGAGC
    CCCAAGCGCT TCCCGTGCGA CTTCGGCCCC GGGGAGGTGC GCTACTCGCA CCTGGGCGCT
    CGCAGCCCGT CGCGGGACCG CGTGCGGCTG CAGCTGCGCT ACGACGCGCC GGGAGGGGCC
    GCAGTGCTAC CGTTGGCGCT GGAGGTGGAG GTGGTCTTCA CCCAGCTGGA GGTCGTGACT
    CGGAACCTGC CCCTCGTCGT GGAGGAGCTG CTGGGGACCA GCAACGCCCT GGACGCGCGG
    AGCCTGGAGT TCGCCTACCA GCCCGAGACC GAGGAGTGTC GCGTGGGCAT CCTGTCGGGC
    TTGAGCGCGC TGCCCCGCTA CGGGGAACTG CTGCGCTACC CGCAGGTTCC CGGTGGAGCC
    GGCGACGGGG GCGCCCAGGA GCCTCTCCTG ATGGACTGCA AGGCCTTCCA GGAGCTGGGC
    GTGCGCTACC GCCACACGGC CCCCGGCCGG TCGCCCAACA GGGACTGGGT GCCCATGGTG
    GTGGAGCTGC GCGCTCGAGG CTCGCCAGTG GGCAGGCCTG CTTTGAAACG GGAGCACTTC
    CAGGTGCTGG TGAGAATCCG AGGGGGGGCG GAGAACACTG CGCCCAAGCC CAGTTTTGTG
    GCCATGATGA TGGTGGAGGT GGATCAGTTT GTCCTGACCG CCCTGACCCC CGACATGCTG
    GCGGCGGAGG ATGCAGAGTC GCCCCCTGAC CTGCTGGTCT TCAACCTCAC CGCTCCCTTC
    CAGCTGGGCC AGGGCTACCT GGTAAGTACC GATGACCGCA GCCTGCCCCT CTCCTCCTTC
    ACTCAGAGGG ATCTGCGGCT CCTGAAGATC GCCTACCAGC CGCCCTCTGA GGACTCTGAC
    CGAGAGCGGC TCTTTGAACT GGAGTTGGAG GTAGTGGATC CAGAAGGAGC AGCCTCAGAC
    CCTTTTGCTT TCATGGTTGT GGTGAAGCCC ATGAACACGC TGGCTCCAGT GGTCACCCGG
    AACACAGGCC TCCTTCTCTA CGAGGGCCAG TCCCGGCCCC TCACAGGCCC CGCAGGCAGT
    GGACCACAAA ACTTGGTCAT CAGCGATGAG GATGACCTGG AGGCAGTGCA GCTGGAGGTG
    GTGGCTGGGC TCCGGCATGG TCACCTTGTC CTCCTGGGTG CCACCCACGG CAACTCGGCC
    CCTAAGACCT TTTCGGTGGC CGAGCTAGCG GCCGGCCAGG TGGTCTACCA TCATGATGAC
    AAAGATGGCT CACTGAGTGA CAACCTTGTA CTTCGAATGG TGGACGGAGG ACGCAGGCAC
    CAGGTGCAGT TCCTGTTTCC CATCACCTTA GTGCCTGTGG ATGACCAGCC ACCAGTCCTC
    AATGCTAACA CGGGGCTGAC ACTGGCGGAA GGTGAAACGG TGCCCATCCT GCCCCTTGCT
    CTAAGTGCAG CTGACATTGA CTCAGAGGAC TCTCTGCTAC TTTTTGTGCT GGAATCGCCC
    TTCTCAGCGA TAGGGCATCT GCTTCTCCGC CAAACTCATC CTCCGCACGA GGAGGAGGAG
    TTTACTGGGA GCCCTTGGAG GAAACAGGGA GAGTTCTATG AGAAAGCAGT GACAGAATGG
    CGGCAGCAGG ACATAAGAGA GGGGAGGCTG TTCTACAGGC ACTCGGGGCC CCATAGTCCT
    GGGCCAGTGA TTGACCAGTT CACGTTTCGC GTCCAAGATA ACCATGACCC CCCTAATCAG
    TCAGGACCGC ACAGGTTTGT GGTGCGCATT CATCCGGTGG ATCGCCTCCC TCCAGAGTTG
    GGCAGTGGCT GTCCCCTGCG GATGGTGGTG CAGGAGTCCC AGCTCACCCC GCTGAGGAAG
    AAGTGGCTGC GCTACACCGA CCTGGACACA GATGACCGGG AACTCCATTA CACGGTGACC
    CAGCCCCCCA TGGACACAGA TGAAAACCAC TCGCCAGCCC CCCTGGGCTC CCTGGTCCTA
    ACAGACCAGC CCTCGGTCGT GGTGGCCCAT TTCACCCAAG CCCAGATCAA CCACCATAAA
    ATTGCTTACA AACCTCCAGA TCAAGAACTA GGAGTGGCTG CCCGAGTGGC CCAGTTCCAG
    TTCCAGGTGG AAGACCAAGC TGGGAATGTG GCCCCAGGCA CCTTCACCCT TTACCTGCAG
    CCAGTGGACA ACCAACCACC TGAGATCCTC AACACTGGTT TCACTGTTCA GGAGAAGGGC
    CACCATGTCC TGAGCGAGAC CGAGTTGCGT GTGGATGACG TAGACACAGA CGTGGCACAC
    ATCTCTTTCA CTCTCACTCA GGCACCCAAA CACGGCCACG TGAGAGTGTC TGGACAGATT
    TTGCACGTAG GGGAGGTCTT CCACTTGGAA GATATAAAAC AGGGTCGGAT TTCCTATGCA
    CATAATGGAG ATGAGTCCCT CACTGACAGC TGCTCCTTGG AGGTCAGCGA CACGCACCAC
    GTGGTGCCAA TCACTCTCAG AGTGAACATC CAGCCAGTGG ATGATGAGGT GCCCATGCTG
    AGCCTTTCTG CTGGCACTCT GGGGTCATAT CTAGATGTTT TAGAAAATGG AGCTACCGAA
    ATCACTGCCA GTGTTATCAA GGGGACTGAT GAGGACACTG ATGACTTGAT GTTGACTTTC
    CTCTTGGAAG ATCCACCCTT ATATGGTGAA ATCCTGGTCA ACGGAGTTCC AGCAGAGCAG
    TTTACCCAAA GAGACATTTT AGAGGGCTCT GTCGTCTATG CCCACACTAG TGGTGAGATA
    GGCCTGTTGC CCAAAGGGGA CTCTTTTAAC CTGAGCCTGT CGGATATGTC TCAAGAATGG
    GTAATAGGTG GCAGTACTAT CCAAGGAGTC ACCGTGTGGG TGACCGTCCT CCCTGTCGAT
    AGCCAGGCTC CAGAAATTTC TATAGGTGAA CAGTTTATAG TTGTGGAAGG TGATAAGAGT
    GTTGTAACAT CAGCACATAT AAGTGCTGAA GATACCGATT CACTAAATGA TGACATCTTA
    TGCACTATAG TTATTCAGCC TACGTCCGGT TATGTTGAAA ACATTTCTCC AGCCCCAGGC
    TCTGAGAAGT CAAGAGCAGG AGTTGCCATA AGTGCCTTTA CCGTGAAAGA CCTCAGGCAG
    AGTCATATTA ACTATGTCCA GAGTGTCCAT AAAGGGGTAG AACCTGTGGA AGATCGATTT
    ATATTTCGTT GCTCTGATGG CATTAACTTT TCAGAGAGAC ATTTATTCCC CATTGTCATC
    ATTCCCACCA ATGATGAACA GCCAGAAATA TTTATGAGAG AATTTATGGT GATGGAAGGC
    ATGAGTCTGG TGATCGACAC GCCCATCCTC AATGCTGCTG ATGCTGACAT TCCCCCCGAT
    GACTTAACTT TTACTGTTAC CCAATTTCCT ACTCGCGGTC ACATTGTGAA CCAGCTGATA
    AATGGCACAG TTTTGGTTGA AAGCTTTACC TTGGACCAGA TCATAGAGAG TTCTAGCATT
    ATTTATGAGC ATGATGACTC TGAGACCCAG GAAGACCGTT TTGTGATTAG ACTAACAGAT
    GGTAGGCATT CAGTGGAAAG GACGGTCCCC ATTATTGTTA TCCCAGTTGA TGATGAGACC
    CCCAGAATGA CCATCAATAA TGGACTAGAA ATAGAAATTG GGGAAACCAA AATTATCAAC
    AACAAAATAT TAATGGCAAC TGATTTAGAT TCGGAAGACA AATCTTTGGT TTATGTTATT
    CGTTATGGGC CAGGACATGG CTTATTGCAG AGGCAAAAAC CTACAGGTGC CTTTGAAAAT
    ATCACACTCG GCATGAATTT TACCCAGGAT GAAGTGGACA GAAACTTAAT TCAGTATGTC
    CATGTTGGAC AAGAGGGCAT TCGGGACCTT ATTAAATTTG ATGTGACTGA CGGAATAAAC
    GCCCTGATAG ATCGCTACTT TTACATTTCC GTCGGGAGTA TTGACACTGT CTTCCCAGAT
    GTGATAAGCA AGGGGGTATC CTTGAAAGAA GGAGGCAAAG TCACCCTCAC AACAGACCTC
    CTAAGCACAA GTGACCTGAA CAGTCCTGAT GAAAACTTAG TTTTCACCAT CACCAGGGCT
    CCCACGAGAG GTCACTTAGA GTGCACAGAT CAACGTGGAG CTTCCATCAT GTCTTTCACT
    CAGCTCCAAC TGGCTGGCAA CAAAATCTAC TACATCCACA CAGCTGATGA TGAGGTCAAG
    ATGGACAGTT TTGAGTTTCA AGTCACGGAT GGACGTAACC CTGTCTTCCG GACATTCCGT
    ATCTCCATTA GTGATGTGGA TAACAAAAAG CCAGTAGTCA CCATCCATAA CCTGGTTGTC
    AGCGAGGGTG AAAACAAGCT GATCACTCCC TTTGAACTCA CTGCCGAAGA CCCAGATACT
    CCTGACAAAC TCCTGAAGTT CATCGTCACC CAGGTGCCTG TTCATGGTCA ACTCTTATTC
    AACAATACCA GACCTGCCAT GGTTTTTACC AAGCAAGACT TGAATGAAAA CTTAATCAGC
    TACAGACATG ATGGCACCGA GTCAAGCGAA GACAGCTTCT CCTTCACGGT GACTGACAGT
    TCTCATACAG AGTTCTATGT CTTCCCTGAT ACAATATTTG AAACGAGGAG ACCCCAAGTG
    ATGAAAATCC AGGTCTTAGC TGTTGACGAT AGCATCCCCC AGATTGCAGT GAACAAAGGG
    GCTTCCACGC TTCGCCCTCT GGCTACCGGC CACTTGGGGT TCATGATCAC AAGCAAAGTA
    TTGAAAGCAG AGGACAGAGA CAGCTTACGT TTTTCTCTCA GGTTTATTGT GACAGGAGCT
    CCTCAACATG GGTATCTCCT CAATCTGGAC AGAGGCAACC ACAGTGTCAC TCAGTTTACA
    CAAGGTGACA TTGATGACAT GAAAATATGC TACATTTTAA AAGAGGGGGC CAATGCCACA
    AGTGATGTGT TCCATTTTAC AGTTGAAGAT GGTGGAGGAA ACAAATTAAC CAAGCAACAT
    TTTCGTCTGA ATTGGGCATG GATCTCCTTT GAAAAGGAAT ATTACCTGAT CAATGAGGAC
    TCCAAATTTC TAGATGTCGT TCTTAAACGT AGAGGGTTTT TGGGAGAGAC TTCATTTATA
    AGTATTGGCA CAAGAGATGG GACTGCAGAA AAAGACAAAG ACTTCAAGGG CAAAGCACAG
    AAACAAGTAC AGTTCAACCC AGGCCAGACC AGGGCAACCT GGAAAGTGCG GATCATGAGC
    GATGGGGAGC ATGAGCAGTC GGAGACCTTC CAGGTGGTTC TCTCAGAGCC GGTGCTGGCT
    GCTTTGGAGT TTCCCACAGT GACCACAGTG GAGATCATTG ACCCAGGAGA TGAGTCAACT
    GTATTTATTC CCCAGTCTGA ATACTCTGTT GAAGAAGACG TCGGTGAGCT GTTCATTCCC
    ATTAGGAGGA GTGGAGACGT GAGCCAGGAG CTGATGGTGA TCTGTTACAC ACAACAAGGA
    ACAGCAGGCG GAACTGTACC AACATCAGTG CTGTCCTATT CAGACTACAT ATCCAGGCCG
    GAGGACCACA CCAGTGTCAT CCGCTTTGAC AAAGATGAAC ACGAGAAAAT GTGTCGCATC
    GTTGTTATTG ATGACTCCCT GTATGAAGAG GAGGAAACAT TCCATGTCCT TCTGAGCATG
    CCCATGGGAG GGAGGATTGG ATCAGAGTTC CCAGGGACTC AGGTTACAAT CATCCCTGAC
    AAAGATGATG AACCTGTCTT CTACTTTGGT GATGTCGAGT ACCTGGTGGA CGAGAGCGCC
    GGCTATGTGG AATTGCGGGT GTGGAGAACA GGCACCGACC TGTCCAGGTC TTCCAGTGTC
    ACCGTGAGGT CTCGGAAAAC AGATCCTCCC TCTGCTGATG CCGGAACAGA TTATGTGGGA
    ATCAGTCGGA ATTTAGATTT TGCACCTGGA GTCAACATGC AGACTGTTCG TGTTATCATC
    CTGGATGATC TTGGGCAACC AGTGCTGGAG GGAATTGAGA AATTTGAACT GGTGCTTCGT
    ATGCCTATGA ACGCTGCCCT CGGCGAGCCC AGCAAAGCCA CGGTTTCCAT AAATGATTCT
    GTCTCCGACT TGCCTAAGAT GCAGTTCAAA GAACGAGTAT ATACTGGCAA TGAAAACGAT
    GGGCAGATAG TTGCAATGAT CCACAGGAGT GGAGACATTC ACTATAGGTC TTCAGTGAGA
    TGCTACACAC GACAGGGGTC TGCACAGGTG ATGACGGACT TTGAGGAACG TCCAAACATT
    GATAGCTCTG TCATCCTGTT CCTCCCTGGT GAGACTGAAA AGCCTTGTGT ACTTGAGCTG
    ATGGATGACA AACTCTATGA AGAGGTAGAG GAACTCCGCC TGGTTCTTGG AACCCCAAGT
    AGCGACTCCC CCTTTGGGGC TGCAGTTGGG GAACAAAATG AAACTCTGAT AAGGATCCAA
    GATGATGCTG ATAAGACTGT TATTAAATTT GGAGAAACCA GATTTAGCGT GAGTGAACCC
    AAGGAACCAG GACAGTCGGT GGTTGTAAAA ATTCCAGTGA TTCGCCAAGG AGATACTTCA
    AAGGTTTCAA TTGTGAGAGT CCACACCAAG GATGGCTCAG CCACTTCTGG AGAAGACTAT
    CACCCTGTGT CAGAAGAAAT TGAATTTAAG GAGGGAGAAA CCCAGCATAT TGTTAACATT
    GAAGTAATCT TCGATGGGAT CAGAGAGATG AGAGAGGCCT TCACTGTCCA CCTAAAACCC
    GATGAGAACA TGGTGGCAGA GACGCAGCCC ATTGTGTATA TAGAAGAAAT GAACAGCATG
    GCGGAAGTCA CTTTCCCATC TGTCCCTCAA ATTGTGTCAT TACTGATGTA TGATGACACT
    TCCAGAGCTA AAGATAGTGC AGGACCTGTG TCTGGCTATC CTGTTGTCTG TATTACAGCT
    TGCAACCCTA AATACTCAGA CTATGACAAA ACAGGTTCTA TTTGTGCAAG TGAAAATATC
    AATGACACCT TGACCCGGTA CCGATGGCTA ATTAGTGCAC CCGCTGGCCC TGATGGTGTG
    ACCAGCCCCA TGAGAGAAGT GGACTTTGAC ACCTTTTTTA CATCGTCCAA GATGATCACC
    TTGGATTCCA TATACTTCCA GCCCGGCTCC AGAGTGCAGT GTGCGGCCCG TGCTGTGAAT
    GCCAATGGCA ATGAGGGCCT GGAGCTGATG AGCCCTATTG TAACCATCGG CAGAGAAGAA
    GGTCTTTGTC AGCCCAGGGT GCCAGGGACA GTGGGAGCAG AGCCATTCTC AGCTAAGCTG
    CGCTACACAG GCCCCGAGGA CCCAGAGTAC ACGAACTTTA TCAAGCTCAC CGTCACGATG
    CCACACATAG ATGGTATGCT CCCTGTGATC TCCACTAAAG AGCTTTCCAA CTTTGAGCTC
    ACCCTCAGTC CTGATGGCTC AAGAGTTGGA AACCACCAGT GCTCCAATCT TCTAGATTAC
    ACTGAAGTGA AGACTCACCA TGGCTTCTTA ACTGATGCTA CGAAAAATCC AGAAGTAATT
    GGAGAGACTC ATCCTTACCA GTACAGCTCA TCTATCAGAG GTTCCAGTAC CTTACGCTTC
    TATCGAAACT TGAACCTGGA AGCCTGTTTA TGGGAGTTTG TTAGCTACTA TGACATGTCA
    GAACTTCTCA CCAACTGTGG AGGAACCATC GGGACAGACG GACAGGTCCT GAATCTGGTG
    CAGTCATATG TGACCCTTCG GGTGCCTCTG TATGTTTCGT ATGTGTTCCA TTCCCCCGTT
    GGCGTAGGGG GCTGGCAGCA TTTTGACTTG AAGTCGGAGC TCCGCCTGAC TTTCGTGTAT
    GACACTGCCA TCTTGTGGAA TGAGGGCATC GGCAGCCCAC CAGAAGCCGA GCTCCAAGGT
    TCTCTCTACC CGACTAGCAT GCGCATTGGT GAAGAGGGGC GCTTGGTTGT GAACTTCAAG
    ACAGAGGCTC AGTTCCATGG ATTGTTTGTG CTGTCTCATC CCGCATCCTT TACAAGCTCA
    ATGATCATGT CAGCTGATCA CCCGGGCCTG ACCTTCTCCT TGCGCCTCAT CAGGAGTGAA
    CCAACCTATA ACCAGCCAGT GCAGCAGTGG AGCTTTGTGT CTGACTTTGC AGTACGTGAC
    TATTCGGGGA CCTACACCGT GAAACTGCTA CCTTGCACTG CTCCATCGCA TCAGGAGTAC
    CACCTGCCAG TCACCTGCAG CCCCAGAGAG CCCATCACCT TTGACCTCGA CATACGATTC
    CAGCAGGTCA GTGATCCAGT GGCGGCTGAG TTTAGCTTGA ACACCCAAAT GTACCTCCTG
    TCCAAGAAGA GTCTCTGGTT GTCTGACGGA TCCATGGGAT TTGGGCAAGA GAGTGATGTT
    GCCTTCACAG AAGGTGATAT AATCTATGGT CGTGTCATGG TAGATCCTGT CCAGAATCTG
    GGTGACTCCT TTTACTGCAG CATTGAGAAG GTGTTCTTGT GCACTGGAGC TGATGGCTAT
    GTTCCCAAAT ATAATCCCAC AAATGCAGAA TATGGCTGCT TAGCCGACTC TCCTTCACTC
    TTATATAGAT TTAAAATTGT GGACAAAGCT CAACCAGAGA CACAAGCCAT CAGTTTTGGA
    AATGTCCTGT TTAATGCCAA ACTAGCAATG GATGATCCTG AAGCTATTCT CTTAGTGAAT
    CAGCCTGGAT CTGATGGATT TAAAGTCGAC TCCATGCCAC TCTTTCAGGT CGCTCTGGGC
    CGAGAATGGT ACATACACAC GGTCTACACA GTAAGATCAA AAGACAACGC CAATCGAGGC
    ATTGGCAAAA GAAGCACTGA GCACCAGCAC CACTCCTTAG TGAGACCAGG GAAGCCCCAG
    GCAACTAGCA AGGGCCGGAA GAAGAGGGAG ATCAGGAGCC CACCCCCACT GGCCTGGCAA
    ATAGGTGCTG AGAACAACCG GGGAACAAAC ATCCAGCACA TTGCCCTGGA CCGCAGCAGC
    CGGAAGCTGA TCCCCCAAGG CCGAGTTCCT CCTGACGGCG TCCTCCCCCG GGAGCTCAAC
    CGTCCCAGCT CTGAGGTCAG CCTGGTCACA GTCGTTGGCG GCATCGCGGT GGGATTGCTA
    ACCCTCTGCC TTGCTGCCAT CACAGTGATG ATGTGCAAGA GCAAGAAGGG CTCCAGGAGA
    AAGGATGCTC TAAAGTGCTC AGGCAGCAGC AAGCCCATGA TGCCTCCGCA GAGCCACCAC
    TGTGACAGCT CAGAGGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.