SERPINH1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SERPINH1
Entrez Gene ID	485187
Full Name	serpin family H member 1
General protein information	Preferred Names serpin family H member 1 Names serpin H1
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005633296.3	XP_005633353.1	serpin H1 isoform X1
NM_001165888.1	NP_001159360.1	serpin H1 precursor
XM_005633296.2	XP_005633353.1	serpin H1 isoform X1

Pathways from BioSystems

Homologies

	SERPINH1	XP_001084827.2
Rattus norvegicus (Norway rat)	Serpinh1	NP_058869.1
Gallus gallus (chicken)	SERPINH1	NP_990622.1
Mus musculus (house mouse)	Serpinh1	NP_001104513.1
Danio rerio (zebrafish)	serpinh1b	NP_571279.1
Xenopus tropicalis (tropical clawed frog)	LOC100490491	XP_004912280.1
Homo sapiens (human)	SERPINH1	NP_001193943.1
Pan troglodytes (chimpanzee)	SERPINH1	NP_001233539.1
Canis lupus familiaris (dog)	SERPINH1	NP_001159360.1
Bos taurus (cattle)	SERPINH1	NP_001039528.1
Danio rerio (zebrafish)	serpinh1a	NP_001103844.1

Gene Ontology
Bibliography

Related articles in PubMed

The pH-dependent Client Release from the Collagen-specific Chaperone HSP47 Is Triggered by a Tandem Histidine Pair.
Oecal S, Socher E, Uthoff M, Ernst C, Zaucke F, Sticht H, Baumann U, Gebauer JM
The Journal of biological chemistry291(24)12612-26(2016 Jun)

Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta.
Lindert U, Weis MA, Rai J, Seeliger F, Hausser I, Leeb T, Eyre D, Rohrbach M, Giunta C
The Journal of biological chemistry290(29)17679-89(2015 Jul)

Population screening for the mutation associated with osteogenesis imperfecta in dachshunds.
Eckardt J, Kluth S, Dierks C, Philipp U, Distl O
The Veterinary record172(14)364(2013 Apr)

Molecular basis for the action of the collagen-specific chaperone Hsp47/SERPINH1 and its structure-specific client recognition.
Widmer C, Gebauer JM, Brunstein E, Rosenbaum S, Zaucke F, Dr?gem?ller C, Leeb T, Baumann U
Proceedings of the National Academy of Sciences of the United States of America109(33)13243-7(2012 Aug)

A missense mutation in the SERPINH1 gene in Dachshunds with osteogenesis imperfecta.
Dr?gem?ller C, Becker D, Brunner A, Haase B, Kircher P, Seeliger F, Fehr M, Baumann U, Lindblad-Toh K, Leeb T
PLoS genetics5(7)e1000579(2009 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that heat shock protein 47 (HSP47/SERPINH1) double mutant with histidine His(273) and His(274) exchanged for asparagines exhibits a much less pronounced pH dependence of collagen binding.
Title: The pH-dependent Client Release from the Collagen-specific Chaperone HSP47 Is Triggered by a Tandem Histidine Pair.

In Osteogenesis Imperfecta dachshunds, an SERPINH1/HSP47 (L326P) mutation affects the post-translational modification, secretion, and cross-linking of collagen type I.
Title: Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta.

Osteogenesis imperfecta (OI) is a genetic disorder caused by a recessive mutation in the SERPINH1 gene causing defects in the development of collagen type I in dachshunds.
Title: Population screening for the mutation associated with osteogenesis imperfecta in dachshunds.

Crystal structures of Hsp47 in its free form and in complex with homotrimeric synthetic collagen model peptides, are presented.
Title: Molecular basis for the action of the collagen-specific chaperone Hsp47/SERPINH1 and its structure-specific client recognition.

A missense mutation (c.977C>T, p.L326P) located in an evolutionary conserved domain was perfectly associated with the Osteogenesis imperfecta phenotype in Dachshunds.
Title: A missense mutation in the SERPINH1 gene in Dachshunds with osteogenesis imperfecta.

The following SERPINH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SERPINH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe22733	NM_001165888.1 Latest version!	Canis lupus familiaris serpin family H member 1 (SERPINH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe22733	XM_005633296.3 Latest version!	Canis lupus familiaris serpin family H member 1 (SERPINH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OCe22733	XM_005633296.2	Canis lupus familiaris serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SERPINH1 ( NM_001165888.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> NP_001159360.1 Canis lupus familiaris serpin family H member 1 (SERPINH1), mRNA.

Price & Availability↑

CloneID	OCe22733
Clone ID Related Accession (Same CDS sequence)	XM_005633296.3 , NM_001165888.1 , XM_005633296.2
Accession Version	NM_001165888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	serpin H1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAEX03012799.1 and AAEX03012798.1. On Sep 25, 2009 this sequence version replaced XM_542305.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: FN395288.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGCGCTTCC TCCTGCTTCT CAACACCTGC TGCCTCCTGG CCGTGGTCCT GGCGGCCGAG 
GTGAAGAAGC CCGCGGCTGC AGCGGCCCCC GGCTCTGCCG AGAAGCTGAG CCCCAAAGCG 
GCCACGCTGG CCGAGCGCAG CGCCGGCTTG GCCTTCAGCC TGTACCAGGC CATGGCCAAG 
GACCAGGCGG TGGAGAACAT CCTGCTGTCA CCCGTGGTAG TGGCCTCGTC CCTGGGGCTC 
GTGTCGCTGG GGGGCAAGGC CACCACGGCA TCCCAGGCCA AGGCGGTGCT GAGCGCGGAG 
CAGCTGCGCG ACGAGGAGGT GCACGCGGGC CTGGGCGAGC TGCTGCGATC GCTCAGCAAC 
AGCACCGCGC GCAACGTGAC GTGGAAGCTG GGCAGCCGCC TGTACGGGCC CAGCTCCGTC 
AGCTTCGCCG AGGACTTCGT GCGCAGCAGC AAGCAGCACT ACAACTGCGA GCACTCCAAG 
ATCAACTTCC GCGACAAGCG CAGCGCCCTG CAGTCCATCA ATGAGTGGGC GGCGCAGACC 
ACCGACGGCA AGCTGCCCGA GGTCACCAAG GACGTGGAGC GCACGGATGG CGCGCTGCTG 
GTCAATGCCA TGTTCTTCAA GCCACACTGG GACGAGAAGT TCCACCACAA GATGGTGGAT 
AACCGAGGCT TCATGGTAAC CCGTTCCTAT ACCGTGGGTG TCACGATGAT GCACCGCACA 
GGCCTCTACA ACTACTACGA TGATGAGAAA GAGAAGCTGC AGATTGTGGA GATGCCCCTG 
GCCCACAAGC TCTCCAGCCT CATCATCCTC ATGCCGCACC ACGTGGAGCC CCTTGAGCGC 
CTTGAGAAGC TGCTGACCAA GGAGCAGCTG AAGATCTGGA TGGGAAAGAT GCAGAAAAAG 
GCTGTTGCCA TCTCCCTGCC CAAGGGTGTG GTGGAGGTGA CCCACGACCT ACAGAAACAC 
CTGGCTGGGC TGGGTCTGAC GGAGGCCATT GACAAGAACA AGGCAGACCT GTCACGCATG 
TCAGGCAAGA AGGACCTGTA CCTGGCCAGT GTGTTCCACG CCACCGCCTT CGAGTGGGAC 
ACGGAGGGCA ACCCCTTCGA CCAGGACATC TACGGGCGCG AGGAGCTGCG CAGCCCCAAG 
CTCTTCTATG CTGACCACCC CTTCATCTTC CTGGTTCGAG ACACCCAGAG TGGCTCCCTG 
CTATTCATTG GGCGCCTGGT CCGGCCCAAG GGTGACAAGA TGCGAGATGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159360.1
CDS	161..1417
Translation	MRFLLLLNTCCLLAVVLAAEVKKPAAAAAPGSAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILLSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFAEDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVTMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFEWDTEGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Target ORF information:

RefSeq Version	NM_001165888.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris serpin family H member 1 (SERPINH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165888.1

ORF Insert Sequence:

ATGCGCTTCC TCCTGCTTCT CAACACCTGC TGCCTCCTGG CCGTGGTCCT GGCGGCCGAG 
GTGAAGAAGC CCGCGGCTGC AGCGGCCCCC GGCTCTGCCG AGAAGCTGAG CCCCAAAGCG 
GCCACGCTGG CCGAGCGCAG CGCCGGCTTG GCCTTCAGCC TGTACCAGGC CATGGCCAAG 
GACCAGGCGG TGGAGAACAT CCTGCTGTCA CCCGTGGTAG TGGCCTCGTC CCTGGGGCTC 
GTGTCGCTGG GGGGCAAGGC CACCACGGCA TCCCAGGCCA AGGCGGTGCT GAGCGCGGAG 
CAGCTGCGCG ACGAGGAGGT GCACGCGGGC CTGGGCGAGC TGCTGCGATC GCTCAGCAAC 
AGCACCGCGC GCAACGTGAC GTGGAAGCTG GGCAGCCGCC TGTACGGGCC CAGCTCCGTC 
AGCTTCGCCG AGGACTTCGT GCGCAGCAGC AAGCAGCACT ACAACTGCGA GCACTCCAAG 
ATCAACTTCC GCGACAAGCG CAGCGCCCTG CAGTCCATCA ATGAGTGGGC GGCGCAGACC 
ACCGACGGCA AGCTGCCCGA GGTCACCAAG GACGTGGAGC GCACGGATGG CGCGCTGCTG 
GTCAATGCCA TGTTCTTCAA GCCACACTGG GACGAGAAGT TCCACCACAA GATGGTGGAT 
AACCGAGGCT TCATGGTAAC CCGTTCCTAT ACCGTGGGTG TCACGATGAT GCACCGCACA 
GGCCTCTACA ACTACTACGA TGATGAGAAA GAGAAGCTGC AGATTGTGGA GATGCCCCTG 
GCCCACAAGC TCTCCAGCCT CATCATCCTC ATGCCGCACC ACGTGGAGCC CCTTGAGCGC 
CTTGAGAAGC TGCTGACCAA GGAGCAGCTG AAGATCTGGA TGGGAAAGAT GCAGAAAAAG 
GCTGTTGCCA TCTCCCTGCC CAAGGGTGTG GTGGAGGTGA CCCACGACCT ACAGAAACAC 
CTGGCTGGGC TGGGTCTGAC GGAGGCCATT GACAAGAACA AGGCAGACCT GTCACGCATG 
TCAGGCAAGA AGGACCTGTA CCTGGCCAGT GTGTTCCACG CCACCGCCTT CGAGTGGGAC 
ACGGAGGGCA ACCCCTTCGA CCAGGACATC TACGGGCGCG AGGAGCTGCG CAGCCCCAAG 
CTCTTCTATG CTGACCACCC CTTCATCTTC CTGGTTCGAG ACACCCAGAG TGGCTCCCTG 
CTATTCATTG GGCGCCTGGT CCGGCCCAAG GGTGACAAGA TGCGAGATGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SERPINH1 ( XM_005633296.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005633353.1 Canis lupus familiaris serpin family H member 1 (SERPINH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22733
Clone ID Related Accession (Same CDS sequence)	XM_005633296.3 , NM_001165888.1 , XM_005633296.2
Accession Version	XM_005633296.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	serpin H1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633296.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTTCC TCCTGCTTCT CAACACCTGC TGCCTCCTGG CCGTGGTCCT GGCGGCCGAG 
GTGAAGAAGC CCGCGGCTGC AGCGGCCCCC GGCTCTGCCG AGAAGCTGAG CCCCAAAGCG 
GCCACGCTGG CCGAGCGCAG CGCCGGCTTG GCCTTCAGCC TGTACCAGGC CATGGCCAAG 
GACCAGGCGG TGGAGAACAT CCTGCTGTCA CCCGTGGTAG TGGCCTCGTC CCTGGGGCTC 
GTGTCGCTGG GGGGCAAGGC CACCACGGCA TCCCAGGCCA AGGCGGTGCT GAGCGCGGAG 
CAGCTGCGCG ACGAGGAGGT GCACGCGGGC CTGGGCGAGC TGCTGCGATC GCTCAGCAAC 
AGCACCGCGC GCAACGTGAC GTGGAAGCTG GGCAGCCGCC TGTACGGGCC CAGCTCCGTC 
AGCTTCGCCG AGGACTTCGT GCGCAGCAGC AAGCAGCACT ACAACTGCGA GCACTCCAAG 
ATCAACTTCC GCGACAAGCG CAGCGCCCTG CAGTCCATCA ATGAGTGGGC GGCGCAGACC 
ACCGACGGCA AGCTGCCCGA GGTCACCAAG GACGTGGAGC GCACGGATGG CGCGCTGCTG 
GTCAATGCCA TGTTCTTCAA GCCACACTGG GACGAGAAGT TCCACCACAA GATGGTGGAT 
AACCGAGGCT TCATGGTAAC CCGTTCCTAT ACCGTGGGTG TCACGATGAT GCACCGCACA 
GGCCTCTACA ACTACTACGA TGATGAGAAA GAGAAGCTGC AGATTGTGGA GATGCCCCTG 
GCCCACAAGC TCTCCAGCCT CATCATCCTC ATGCCGCACC ACGTGGAGCC CCTTGAGCGC 
CTTGAGAAGC TGCTGACCAA GGAGCAGCTG AAGATCTGGA TGGGAAAGAT GCAGAAAAAG 
GCTGTTGCCA TCTCCCTGCC CAAGGGTGTG GTGGAGGTGA CCCACGACCT ACAGAAACAC 
CTGGCTGGGC TGGGTCTGAC GGAGGCCATT GACAAGAACA AGGCAGACCT GTCACGCATG 
TCAGGCAAGA AGGACCTGTA CCTGGCCAGT GTGTTCCACG CCACCGCCTT CGAGTGGGAC 
ACGGAGGGCA ACCCCTTCGA CCAGGACATC TACGGGCGCG AGGAGCTGCG CAGCCCCAAG 
CTCTTCTATG CTGACCACCC CTTCATCTTC CTGGTTCGAG ACACCCAGAG TGGCTCCCTG 
CTATTCATTG GGCGCCTGGT CCGGCCCAAG GGTGACAAGA TGCGAGATGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633353.1
CDS	229..1485
Translation	MRFLLLLNTCCLLAVVLAAEVKKPAAAAAPGSAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILLSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFAEDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVTMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFEWDTEGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Target ORF information:

RefSeq Version	XM_005633296.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris serpin family H member 1 (SERPINH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633296.3

ORF Insert Sequence:

ATGCGCTTCC TCCTGCTTCT CAACACCTGC TGCCTCCTGG CCGTGGTCCT GGCGGCCGAG 
GTGAAGAAGC CCGCGGCTGC AGCGGCCCCC GGCTCTGCCG AGAAGCTGAG CCCCAAAGCG 
GCCACGCTGG CCGAGCGCAG CGCCGGCTTG GCCTTCAGCC TGTACCAGGC CATGGCCAAG 
GACCAGGCGG TGGAGAACAT CCTGCTGTCA CCCGTGGTAG TGGCCTCGTC CCTGGGGCTC 
GTGTCGCTGG GGGGCAAGGC CACCACGGCA TCCCAGGCCA AGGCGGTGCT GAGCGCGGAG 
CAGCTGCGCG ACGAGGAGGT GCACGCGGGC CTGGGCGAGC TGCTGCGATC GCTCAGCAAC 
AGCACCGCGC GCAACGTGAC GTGGAAGCTG GGCAGCCGCC TGTACGGGCC CAGCTCCGTC 
AGCTTCGCCG AGGACTTCGT GCGCAGCAGC AAGCAGCACT ACAACTGCGA GCACTCCAAG 
ATCAACTTCC GCGACAAGCG CAGCGCCCTG CAGTCCATCA ATGAGTGGGC GGCGCAGACC 
ACCGACGGCA AGCTGCCCGA GGTCACCAAG GACGTGGAGC GCACGGATGG CGCGCTGCTG 
GTCAATGCCA TGTTCTTCAA GCCACACTGG GACGAGAAGT TCCACCACAA GATGGTGGAT 
AACCGAGGCT TCATGGTAAC CCGTTCCTAT ACCGTGGGTG TCACGATGAT GCACCGCACA 
GGCCTCTACA ACTACTACGA TGATGAGAAA GAGAAGCTGC AGATTGTGGA GATGCCCCTG 
GCCCACAAGC TCTCCAGCCT CATCATCCTC ATGCCGCACC ACGTGGAGCC CCTTGAGCGC 
CTTGAGAAGC TGCTGACCAA GGAGCAGCTG AAGATCTGGA TGGGAAAGAT GCAGAAAAAG 
GCTGTTGCCA TCTCCCTGCC CAAGGGTGTG GTGGAGGTGA CCCACGACCT ACAGAAACAC 
CTGGCTGGGC TGGGTCTGAC GGAGGCCATT GACAAGAACA AGGCAGACCT GTCACGCATG 
TCAGGCAAGA AGGACCTGTA CCTGGCCAGT GTGTTCCACG CCACCGCCTT CGAGTGGGAC 
ACGGAGGGCA ACCCCTTCGA CCAGGACATC TACGGGCGCG AGGAGCTGCG CAGCCCCAAG 
CTCTTCTATG CTGACCACCC CTTCATCTTC CTGGTTCGAG ACACCCAGAG TGGCTCCCTG 
CTATTCATTG GGCGCCTGGT CCGGCCCAAG GGTGACAAGA TGCGAGATGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SERPINH1 ( XM_005633296.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005633353.1 Canis lupus familiaris serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22733
Clone ID Related Accession (Same CDS sequence)	XM_005633296.3 , NM_001165888.1 , XM_005633296.2
Accession Version	XM_005633296.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	serpin H1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633296.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTTCC TCCTGCTTCT CAACACCTGC TGCCTCCTGG CCGTGGTCCT GGCGGCCGAG 
GTGAAGAAGC CCGCGGCTGC AGCGGCCCCC GGCTCTGCCG AGAAGCTGAG CCCCAAAGCG 
GCCACGCTGG CCGAGCGCAG CGCCGGCTTG GCCTTCAGCC TGTACCAGGC CATGGCCAAG 
GACCAGGCGG TGGAGAACAT CCTGCTGTCA CCCGTGGTAG TGGCCTCGTC CCTGGGGCTC 
GTGTCGCTGG GGGGCAAGGC CACCACGGCA TCCCAGGCCA AGGCGGTGCT GAGCGCGGAG 
CAGCTGCGCG ACGAGGAGGT GCACGCGGGC CTGGGCGAGC TGCTGCGATC GCTCAGCAAC 
AGCACCGCGC GCAACGTGAC GTGGAAGCTG GGCAGCCGCC TGTACGGGCC CAGCTCCGTC 
AGCTTCGCCG AGGACTTCGT GCGCAGCAGC AAGCAGCACT ACAACTGCGA GCACTCCAAG 
ATCAACTTCC GCGACAAGCG CAGCGCCCTG CAGTCCATCA ATGAGTGGGC GGCGCAGACC 
ACCGACGGCA AGCTGCCCGA GGTCACCAAG GACGTGGAGC GCACGGATGG CGCGCTGCTG 
GTCAATGCCA TGTTCTTCAA GCCACACTGG GACGAGAAGT TCCACCACAA GATGGTGGAT 
AACCGAGGCT TCATGGTAAC CCGTTCCTAT ACCGTGGGTG TCACGATGAT GCACCGCACA 
GGCCTCTACA ACTACTACGA TGATGAGAAA GAGAAGCTGC AGATTGTGGA GATGCCCCTG 
GCCCACAAGC TCTCCAGCCT CATCATCCTC ATGCCGCACC ACGTGGAGCC CCTTGAGCGC 
CTTGAGAAGC TGCTGACCAA GGAGCAGCTG AAGATCTGGA TGGGAAAGAT GCAGAAAAAG 
GCTGTTGCCA TCTCCCTGCC CAAGGGTGTG GTGGAGGTGA CCCACGACCT ACAGAAACAC 
CTGGCTGGGC TGGGTCTGAC GGAGGCCATT GACAAGAACA AGGCAGACCT GTCACGCATG 
TCAGGCAAGA AGGACCTGTA CCTGGCCAGT GTGTTCCACG CCACCGCCTT CGAGTGGGAC 
ACGGAGGGCA ACCCCTTCGA CCAGGACATC TACGGGCGCG AGGAGCTGCG CAGCCCCAAG 
CTCTTCTATG CTGACCACCC CTTCATCTTC CTGGTTCGAG ACACCCAGAG TGGCTCCCTG 
CTATTCATTG GGCGCCTGGT CCGGCCCAAG GGTGACAAGA TGCGAGATGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633353.1
CDS	249..1505
Translation	MRFLLLLNTCCLLAVVLAAEVKKPAAAAAPGSAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILLSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFAEDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVTMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFEWDTEGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Target ORF information:

RefSeq Version	XM_005633296.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) (SERPINH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633296.2

ORF Insert Sequence:

ATGCGCTTCC TCCTGCTTCT CAACACCTGC TGCCTCCTGG CCGTGGTCCT GGCGGCCGAG 
GTGAAGAAGC CCGCGGCTGC AGCGGCCCCC GGCTCTGCCG AGAAGCTGAG CCCCAAAGCG 
GCCACGCTGG CCGAGCGCAG CGCCGGCTTG GCCTTCAGCC TGTACCAGGC CATGGCCAAG 
GACCAGGCGG TGGAGAACAT CCTGCTGTCA CCCGTGGTAG TGGCCTCGTC CCTGGGGCTC 
GTGTCGCTGG GGGGCAAGGC CACCACGGCA TCCCAGGCCA AGGCGGTGCT GAGCGCGGAG 
CAGCTGCGCG ACGAGGAGGT GCACGCGGGC CTGGGCGAGC TGCTGCGATC GCTCAGCAAC 
AGCACCGCGC GCAACGTGAC GTGGAAGCTG GGCAGCCGCC TGTACGGGCC CAGCTCCGTC 
AGCTTCGCCG AGGACTTCGT GCGCAGCAGC AAGCAGCACT ACAACTGCGA GCACTCCAAG 
ATCAACTTCC GCGACAAGCG CAGCGCCCTG CAGTCCATCA ATGAGTGGGC GGCGCAGACC 
ACCGACGGCA AGCTGCCCGA GGTCACCAAG GACGTGGAGC GCACGGATGG CGCGCTGCTG 
GTCAATGCCA TGTTCTTCAA GCCACACTGG GACGAGAAGT TCCACCACAA GATGGTGGAT 
AACCGAGGCT TCATGGTAAC CCGTTCCTAT ACCGTGGGTG TCACGATGAT GCACCGCACA 
GGCCTCTACA ACTACTACGA TGATGAGAAA GAGAAGCTGC AGATTGTGGA GATGCCCCTG 
GCCCACAAGC TCTCCAGCCT CATCATCCTC ATGCCGCACC ACGTGGAGCC CCTTGAGCGC 
CTTGAGAAGC TGCTGACCAA GGAGCAGCTG AAGATCTGGA TGGGAAAGAT GCAGAAAAAG 
GCTGTTGCCA TCTCCCTGCC CAAGGGTGTG GTGGAGGTGA CCCACGACCT ACAGAAACAC 
CTGGCTGGGC TGGGTCTGAC GGAGGCCATT GACAAGAACA AGGCAGACCT GTCACGCATG 
TCAGGCAAGA AGGACCTGTA CCTGGCCAGT GTGTTCCACG CCACCGCCTT CGAGTGGGAC 
ACGGAGGGCA ACCCCTTCGA CCAGGACATC TACGGGCGCG AGGAGCTGCG CAGCCCCAAG 
CTCTTCTATG CTGACCACCC CTTCATCTTC CTGGTTCGAG ACACCCAGAG TGGCTCCCTG 
CTATTCATTG GGCGCCTGGT CCGGCCCAAG GGTGACAAGA TGCGAGATGA GCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The pH-dependent Client Release from the Collagen-specific Chaperone HSP47 Is Triggered by a Tandem Histidine Pair.
The Journal of biological chemistry291(24)12612-26(2016 Jun)
Oecal S,Socher E,Uthoff M,Ernst C,Zaucke F,Sticht H,Baumann U,Gebauer JM

Molecular Consequences of the SERPINH1/HSP47 Mutation in the Dachshund Natural Model of Osteogenesis Imperfecta.
The Journal of biological chemistry290(29)17679-89(2015 Jul)
Lindert U,Weis MA,Rai J,Seeliger F,Hausser I,Leeb T,Eyre D,Rohrbach M,Giunta C

Population screening for the mutation associated with osteogenesis imperfecta in dachshunds.
The Veterinary record172(14)364(2013 Apr)
Eckardt J,Kluth S,Dierks C,Philipp U,Distl O

Molecular basis for the action of the collagen-specific chaperone Hsp47/SERPINH1 and its structure-specific client recognition.
Proceedings of the National Academy of Sciences of the United States of America109(33)13243-7(2012 Aug)
Widmer C,Gebauer JM,Brunstein E,Rosenbaum S,Zaucke F,Dr?gem?ller C,Leeb T,Baumann U

A missense mutation in the SERPINH1 gene in Dachshunds with osteogenesis imperfecta.
PLoS genetics5(7)e1000579(2009 Jul)
Dr?gem?ller C,Becker D,Brunner A,Haase B,Kircher P,Seeliger F,Fehr M,Baumann U,Lindblad-Toh K,Leeb T

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SERPINH1 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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