ME3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ME3
Entrez Gene ID	485151
Full Name	malic enzyme 3
General protein information	Preferred Names malic enzyme 3 Names NADP-dependent malic enzyme, mitochondrial
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005633386.1	XP_005633443.1	NADP-dependent malic enzyme, mitochondrial
XM_005633385.2	XP_005633442.1	NADP-dependent malic enzyme, mitochondrial
XM_005633384.2	XP_005633441.1	NADP-dependent malic enzyme, mitochondrial
XM_005633384.3	XP_005633441.1	NADP-dependent malic enzyme, mitochondrial isoform X1
XM_022407545.1	XP_022263253.1	NADP-dependent malic enzyme, mitochondrial isoform X3
XM_022407544.1	XP_022263252.1	NADP-dependent malic enzyme, mitochondrial isoform X2
XM_022407546.1	XP_022263254.1	NADP-dependent malic enzyme, mitochondrial isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ME3	NP_006671.2
Oryza sativa (rice)	Os01g0743500	NP_001044219.1
Pan troglodytes (chimpanzee)	ME3	XP_508682.2
Mus musculus (house mouse)	Me3	NP_852072.2
Gallus gallus (chicken)	ME3	XP_003640618.2
Canis lupus familiaris (dog)	ME3	XP_005633441.1
	ME3	XP_001103502.1
Bos taurus (cattle)	ME3	NP_001069345.1
Danio rerio (zebrafish)	me3	NP_001082825.1
Drosophila melanogaster (fruit fly)	Men-b	NP_651959.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004159	XP_003436731.1
Xenopus tropicalis (tropical clawed frog)	me3	NP_001107309.1
Rattus norvegicus (Norway rat)	Me3	NP_001101961.2
Caenorhabditis elegans (roundworm)	men-1	NP_496968.1
Arabidopsis thaliana (thale cress)	NADP-ME1	NP_179580.1

Gene Ontology

The following ME3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ME3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe295909	XM_022407545.1 Latest version!	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe295910	XM_022407546.1 Latest version!	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe22814	XM_005633385.2 Latest version!	Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe22814	XM_005633386.1 Latest version!	Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe22814	XM_005633384.3 Latest version!	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe295908	XM_022407544.1 Latest version!	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OCe22814	XM_005633384.2	Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ME3 ( XM_022407545.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263253.1 Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe295909
Clone ID Related Accession (Same CDS sequence)	XM_022407545.1
Accession Version	XM_022407545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NADP-dependent malic enzyme, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAG GGATGGCCTT TACACTTGAA GAAAGGCTGC AGCTTGGAAT CCATGGCCTG 
ATCCCTCCCT GCTTCCTGAG CCAGGACGTC CAGCTTCTCC GGATCATGAG ATACTATGAG 
CGGCAGCAGA GTGACCTGGA CAAGTACATC ATTCTCATGA CGCTCCAAGA CCGGAATGAG 
AAGCTCTTCT ACCGAGTGCT GACATCAGAT GTGGAGAAAT TCATGCCCAT CGTATACACT 
CCCACTGTGG GGCTGGCCTG TCAGCAGTAT GGCCTGACCT TCCGCAGGCC CCGTGGGCTG 
TTCATCACCA TTCATGACAA AGGCCATCTT GCAACAATGC TGAACTCTTG GCCAGAAGAC 
GATATTAAGG CCGTGGTGGT AACCGATGGG GAGCGCATCC TGGGCCTGGG AGACCTGGGC 
TGCTATGGCA TGGGCATCCC CGTGGGCAAG CTGGCCCTGT ACACGGCATG TGGAGGGGTC 
AGCCCGCAGC AATGCCTCCC CGTCCTGCTG GATGTCGGCA CCAACAACGA GGAGCTGCTC 
AGAGACCCTC TGTACATTGG GCTGAAACAC CAGCGCGTGC GTGGGAAGGA GTACGATGAC 
CTGCTGGACG AGTTCATGCA GGCTGTGACA GACAAGTTTG GAATAAATTG CCTCATCCAA 
TTTGAAGACT TTGCCAATGC CAATGCCTTC CGTCTGCTCA ACAAATACCG CAACAAGTAC 
TGCATGTTCA ATGATGACAT CCAAGGCACA GCCTCCGTCG CTGTGGCAGG GATCCTGGCT 
GCTCTACGAA TCACTAAGAA CAAGCTCTCC AATCATGTGT TTGTTTTCCA AGGTGCAGGT 
GAGGCAGCCA TGGGCATCGC ACACCTCCTT GTCATGGCCC TGGAAAAAGA AGGTGTACCC 
AAGGCGGAGG CTACAAGGAA GATCTGGATG GTGGACTCCA AGGGACTCAT TGTCAAGGGG 
AGGAGCCACC TAAACCATGA GAAGGAGATG TTTGCCCAGG ACCATCCTGA AGTGAAGAGC 
CTGGAGGAGG TGGTGAGGCT GGTGAAGCCC ACGGCCATCA TAGGTGTTGC TGCCATCGCA 
GGAGCCTTCA CGGAGCAGAT TCTGAGGGAC ATGGCCTCCT TCCACGAGCG CCCTATCATC 
TTTGCCCTGA GCAACCCCAC CAGCAAGGCC GAGTGCACGG CTGAGAAGTG CTACCGGGTC 
ACTGAGGGCC GAGGGATCTT TGCCAGTGGA AGTCCTTTTA AGAGCGTGAC TCTAGAAGAT 
GGCAGGACCT TCATTCCTGG GCAAGGAAAC AATGCCTATG TGTTCCCTGG AGTGGCTCTG 
GGAGTCATTG CTGGCGGGAT CCGGCACATC CCAGATGAGA TCTTCCTCCT GACGGCAGAG 
CAAATTGCCC AAGAGGTCTC TGAGCAGCAT TTGTCCCAGG GGAGATTGTA CCCACCACTC 
AGCACCATCC GAGATGTGTC TCTGAGAATC GCCATCAAAG TCCTCGACTT CGCGTACAAA 
CACAACCTGG CCTCCTACTA CCCGGAGCCC AAGGACAAGG AGGCTTTTGT AAGATCCCTG 
GTCTACACTC CAGATTATGA CTCCTTTACA CTGGACAGCT ATACTTGGCC CAAGGAAGCC 
ATGAATGTTC AAACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263253.1
CDS	16..1656
Translation	MAEGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVSPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_022407545.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407545.1

ORF Insert Sequence:

ATGGCAGAAG GGATGGCCTT TACACTTGAA GAAAGGCTGC AGCTTGGAAT CCATGGCCTG 
ATCCCTCCCT GCTTCCTGAG CCAGGACGTC CAGCTTCTCC GGATCATGAG ATACTATGAG 
CGGCAGCAGA GTGACCTGGA CAAGTACATC ATTCTCATGA CGCTCCAAGA CCGGAATGAG 
AAGCTCTTCT ACCGAGTGCT GACATCAGAT GTGGAGAAAT TCATGCCCAT CGTATACACT 
CCCACTGTGG GGCTGGCCTG TCAGCAGTAT GGCCTGACCT TCCGCAGGCC CCGTGGGCTG 
TTCATCACCA TTCATGACAA AGGCCATCTT GCAACAATGC TGAACTCTTG GCCAGAAGAC 
GATATTAAGG CCGTGGTGGT AACCGATGGG GAGCGCATCC TGGGCCTGGG AGACCTGGGC 
TGCTATGGCA TGGGCATCCC CGTGGGCAAG CTGGCCCTGT ACACGGCATG TGGAGGGGTC 
AGCCCGCAGC AATGCCTCCC CGTCCTGCTG GATGTCGGCA CCAACAACGA GGAGCTGCTC 
AGAGACCCTC TGTACATTGG GCTGAAACAC CAGCGCGTGC GTGGGAAGGA GTACGATGAC 
CTGCTGGACG AGTTCATGCA GGCTGTGACA GACAAGTTTG GAATAAATTG CCTCATCCAA 
TTTGAAGACT TTGCCAATGC CAATGCCTTC CGTCTGCTCA ACAAATACCG CAACAAGTAC 
TGCATGTTCA ATGATGACAT CCAAGGCACA GCCTCCGTCG CTGTGGCAGG GATCCTGGCT 
GCTCTACGAA TCACTAAGAA CAAGCTCTCC AATCATGTGT TTGTTTTCCA AGGTGCAGGT 
GAGGCAGCCA TGGGCATCGC ACACCTCCTT GTCATGGCCC TGGAAAAAGA AGGTGTACCC 
AAGGCGGAGG CTACAAGGAA GATCTGGATG GTGGACTCCA AGGGACTCAT TGTCAAGGGG 
AGGAGCCACC TAAACCATGA GAAGGAGATG TTTGCCCAGG ACCATCCTGA AGTGAAGAGC 
CTGGAGGAGG TGGTGAGGCT GGTGAAGCCC ACGGCCATCA TAGGTGTTGC TGCCATCGCA 
GGAGCCTTCA CGGAGCAGAT TCTGAGGGAC ATGGCCTCCT TCCACGAGCG CCCTATCATC 
TTTGCCCTGA GCAACCCCAC CAGCAAGGCC GAGTGCACGG CTGAGAAGTG CTACCGGGTC 
ACTGAGGGCC GAGGGATCTT TGCCAGTGGA AGTCCTTTTA AGAGCGTGAC TCTAGAAGAT 
GGCAGGACCT TCATTCCTGG GCAAGGAAAC AATGCCTATG TGTTCCCTGG AGTGGCTCTG 
GGAGTCATTG CTGGCGGGAT CCGGCACATC CCAGATGAGA TCTTCCTCCT GACGGCAGAG 
CAAATTGCCC AAGAGGTCTC TGAGCAGCAT TTGTCCCAGG GGAGATTGTA CCCACCACTC 
AGCACCATCC GAGATGTGTC TCTGAGAATC GCCATCAAAG TCCTCGACTT CGCGTACAAA 
CACAACCTGG CCTCCTACTA CCCGGAGCCC AAGGACAAGG AGGCTTTTGT AAGATCCCTG 
GTCTACACTC CAGATTATGA CTCCTTTACA CTGGACAGCT ATACTTGGCC CAAGGAAGCC 
ATGAATGTTC AAACAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME3 ( XM_022407546.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263254.1 Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe295910
Clone ID Related Accession (Same CDS sequence)	XM_022407546.1
Accession Version	XM_022407546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NADP-dependent malic enzyme, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTA CACTTGAAGA AAGGCTGCAG CTTGGAATCC ATGGCCTGAT CCCTCCCTGC 
TTCCTGAGCC AGGACGTCCA GCTTCTCCGG ATCATGAGAT ACTATGAGCG GCAGCAGAGT 
GACCTGGACA AGTACATCAT TCTCATGACG CTCCAAGACC GGAATGAGAA GCTCTTCTAC 
CGAGTGCTGA CATCAGATGT GGAGAAATTC ATGCCCATCG TATACACTCC CACTGTGGGG 
CTGGCCTGTC AGCAGTATGG CCTGACCTTC CGCAGGCCCC GTGGGCTGTT CATCACCATT 
CATGACAAAG GCCATCTTGC AACAATGCTG AACTCTTGGC CAGAAGACGA TATTAAGGCC 
GTGGTGGTAA CCGATGGGGA GCGCATCCTG GGCCTGGGAG ACCTGGGCTG CTATGGCATG 
GGCATCCCCG TGGGCAAGCT GGCCCTGTAC ACGGCATGTG GAGGGGTCAG CCCGCAGCAA 
TGCCTCCCCG TCCTGCTGGA TGTCGGCACC AACAACGAGG AGCTGCTCAG AGACCCTCTG 
TACATTGGGC TGAAACACCA GCGCGTGCGT GGGAAGGAGT ACGATGACCT GCTGGACGAG 
TTCATGCAGG CTGTGACAGA CAAGTTTGGA ATAAATTGCC TCATCCAATT TGAAGACTTT 
GCCAATGCCA ATGCCTTCCG TCTGCTCAAC AAATACCGCA ACAAGTACTG CATGTTCAAT 
GATGACATCC AAGGCACAGC CTCCGTCGCT GTGGCAGGGA TCCTGGCTGC TCTACGAATC 
ACTAAGAACA AGCTCTCCAA TCATGTGTTT GTTTTCCAAG GTGCAGGTGA GGCAGCCATG 
GGCATCGCAC ACCTCCTTGT CATGGCCCTG GAAAAAGAAG GTGTACCCAA GGCGGAGGCT 
ACAAGGAAGA TCTGGATGGT GGACTCCAAG GGACTCATTG TCAAGGGGAG GAGCCACCTA 
AACCATGAGA AGGAGATGTT TGCCCAGGAC CATCCTGAAG TGAAGAGCCT GGAGGAGGTG 
GTGAGGCTGG TGAAGCCCAC GGCCATCATA GGTGTTGCTG CCATCGCAGG AGCCTTCACG 
GAGCAGATTC TGAGGGACAT GGCCTCCTTC CACGAGCGCC CTATCATCTT TGCCCTGAGC 
AACCCCACCA GCAAGGCCGA GTGCACGGCT GAGAAGTGCT ACCGGGTCAC TGAGGGCCGA 
GGGATCTTTG CCAGTGGAAG TCCTTTTAAG AGCGTGACTC TAGAAGATGG CAGGACCTTC 
ATTCCTGGGC AAGGAAACAA TGCCTATGTG TTCCCTGGAG TGGCTCTGGG AGTCATTGCT 
GGCGGGATCC GGCACATCCC AGATGAGATC TTCCTCCTGA CGGCAGAGCA AATTGCCCAA 
GAGGTCTCTG AGCAGCATTT GTCCCAGGGG AGATTGTACC CACCACTCAG CACCATCCGA 
GATGTGTCTC TGAGAATCGC CATCAAAGTC CTCGACTTCG CGTACAAACA CAACCTGGCC 
TCCTACTACC CGGAGCCCAA GGACAAGGAG GCTTTTGTAA GATCCCTGGT CTACACTCCA 
GATTATGACT CCTTTACACT GGACAGCTAT ACTTGGCCCA AGGAAGCCAT GAATGTTCAA 
ACAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263254.1
CDS	59..1687
Translation	MAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVSPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_022407546.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407546.1

ORF Insert Sequence:

ATGGCCTTTA CACTTGAAGA AAGGCTGCAG CTTGGAATCC ATGGCCTGAT CCCTCCCTGC 
TTCCTGAGCC AGGACGTCCA GCTTCTCCGG ATCATGAGAT ACTATGAGCG GCAGCAGAGT 
GACCTGGACA AGTACATCAT TCTCATGACG CTCCAAGACC GGAATGAGAA GCTCTTCTAC 
CGAGTGCTGA CATCAGATGT GGAGAAATTC ATGCCCATCG TATACACTCC CACTGTGGGG 
CTGGCCTGTC AGCAGTATGG CCTGACCTTC CGCAGGCCCC GTGGGCTGTT CATCACCATT 
CATGACAAAG GCCATCTTGC AACAATGCTG AACTCTTGGC CAGAAGACGA TATTAAGGCC 
GTGGTGGTAA CCGATGGGGA GCGCATCCTG GGCCTGGGAG ACCTGGGCTG CTATGGCATG 
GGCATCCCCG TGGGCAAGCT GGCCCTGTAC ACGGCATGTG GAGGGGTCAG CCCGCAGCAA 
TGCCTCCCCG TCCTGCTGGA TGTCGGCACC AACAACGAGG AGCTGCTCAG AGACCCTCTG 
TACATTGGGC TGAAACACCA GCGCGTGCGT GGGAAGGAGT ACGATGACCT GCTGGACGAG 
TTCATGCAGG CTGTGACAGA CAAGTTTGGA ATAAATTGCC TCATCCAATT TGAAGACTTT 
GCCAATGCCA ATGCCTTCCG TCTGCTCAAC AAATACCGCA ACAAGTACTG CATGTTCAAT 
GATGACATCC AAGGCACAGC CTCCGTCGCT GTGGCAGGGA TCCTGGCTGC TCTACGAATC 
ACTAAGAACA AGCTCTCCAA TCATGTGTTT GTTTTCCAAG GTGCAGGTGA GGCAGCCATG 
GGCATCGCAC ACCTCCTTGT CATGGCCCTG GAAAAAGAAG GTGTACCCAA GGCGGAGGCT 
ACAAGGAAGA TCTGGATGGT GGACTCCAAG GGACTCATTG TCAAGGGGAG GAGCCACCTA 
AACCATGAGA AGGAGATGTT TGCCCAGGAC CATCCTGAAG TGAAGAGCCT GGAGGAGGTG 
GTGAGGCTGG TGAAGCCCAC GGCCATCATA GGTGTTGCTG CCATCGCAGG AGCCTTCACG 
GAGCAGATTC TGAGGGACAT GGCCTCCTTC CACGAGCGCC CTATCATCTT TGCCCTGAGC 
AACCCCACCA GCAAGGCCGA GTGCACGGCT GAGAAGTGCT ACCGGGTCAC TGAGGGCCGA 
GGGATCTTTG CCAGTGGAAG TCCTTTTAAG AGCGTGACTC TAGAAGATGG CAGGACCTTC 
ATTCCTGGGC AAGGAAACAA TGCCTATGTG TTCCCTGGAG TGGCTCTGGG AGTCATTGCT 
GGCGGGATCC GGCACATCCC AGATGAGATC TTCCTCCTGA CGGCAGAGCA AATTGCCCAA 
GAGGTCTCTG AGCAGCATTT GTCCCAGGGG AGATTGTACC CACCACTCAG CACCATCCGA 
GATGTGTCTC TGAGAATCGC CATCAAAGTC CTCGACTTCG CGTACAAACA CAACCTGGCC 
TCCTACTACC CGGAGCCCAA GGACAAGGAG GCTTTTGTAA GATCCCTGGT CTACACTCCA 
GATTATGACT CCTTTACACT GGACAGCTAT ACTTGGCCCA AGGAAGCCAT GAATGTTCAA 
ACAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME3 ( XM_005633386.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633443.1 Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe22814
Clone ID Related Accession (Same CDS sequence)	XM_005633386.1 , XM_005633385.2 , XM_005633384.2 , XM_005633384.3
Accession Version	XM_005633386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	NADP-dependent malic enzyme, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633443.1
CDS	113..1927
Translation	MGAALGRGARPGLLRGKAWGAVPRWTPSAPGRGCRSRAGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVSPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_005633386.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633386.1

ORF Insert Sequence:

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME3 ( XM_005633385.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633442.1 Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe22814
Clone ID Related Accession (Same CDS sequence)	XM_005633386.1 , XM_005633385.2 , XM_005633384.2 , XM_005633384.3
Accession Version	XM_005633385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	NADP-dependent malic enzyme, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633442.1
CDS	33..1847
Translation	MGAALGRGARPGLLRGKAWGAVPRWTPSAPGRGCRSRAGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVSPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_005633385.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633385.2

ORF Insert Sequence:

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME3 ( XM_005633384.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633441.1 Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22814
Clone ID Related Accession (Same CDS sequence)	XM_005633386.1 , XM_005633385.2 , XM_005633384.2 , XM_005633384.3
Accession Version	XM_005633384.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	NADP-dependent malic enzyme, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726097.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005633384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633441.1
CDS	342..2156
Translation	MGAALGRGARPGLLRGKAWGAVPRWTPSAPGRGCRSRAGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVSPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_005633384.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633384.2

ORF Insert Sequence:

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME3 ( XM_005633384.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005633441.1 Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe22814
Clone ID Related Accession (Same CDS sequence)	XM_005633386.1 , XM_005633385.2 , XM_005633384.2 , XM_005633384.3
Accession Version	XM_005633384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NADP-dependent malic enzyme, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005633384.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005633441.1
CDS	274..2088
Translation	MGAALGRGARPGLLRGKAWGAVPRWTPSAPGRGCRSRAGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVSPQQCLPVLLDVGTNNEELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_005633384.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005633384.3

ORF Insert Sequence:

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGCCGTGG TGGTAACCGA TGGGGAGCGC ATCCTGGGCC TGGGAGACCT GGGCTGCTAT 
GGCATGGGCA TCCCCGTGGG CAAGCTGGCC CTGTACACGG CATGTGGAGG GGTCAGCCCG 
CAGCAATGCC TCCCCGTCCT GCTGGATGTC GGCACCAACA ACGAGGAGCT GCTCAGAGAC 
CCTCTGTACA TTGGGCTGAA ACACCAGCGC GTGCGTGGGA AGGAGTACGA TGACCTGCTG 
GACGAGTTCA TGCAGGCTGT GACAGACAAG TTTGGAATAA ATTGCCTCAT CCAATTTGAA 
GACTTTGCCA ATGCCAATGC CTTCCGTCTG CTCAACAAAT ACCGCAACAA GTACTGCATG 
TTCAATGATG ACATCCAAGG CACAGCCTCC GTCGCTGTGG CAGGGATCCT GGCTGCTCTA 
CGAATCACTA AGAACAAGCT CTCCAATCAT GTGTTTGTTT TCCAAGGTGC AGGTGAGGCA 
GCCATGGGCA TCGCACACCT CCTTGTCATG GCCCTGGAAA AAGAAGGTGT ACCCAAGGCG 
GAGGCTACAA GGAAGATCTG GATGGTGGAC TCCAAGGGAC TCATTGTCAA GGGGAGGAGC 
CACCTAAACC ATGAGAAGGA GATGTTTGCC CAGGACCATC CTGAAGTGAA GAGCCTGGAG 
GAGGTGGTGA GGCTGGTGAA GCCCACGGCC ATCATAGGTG TTGCTGCCAT CGCAGGAGCC 
TTCACGGAGC AGATTCTGAG GGACATGGCC TCCTTCCACG AGCGCCCTAT CATCTTTGCC 
CTGAGCAACC CCACCAGCAA GGCCGAGTGC ACGGCTGAGA AGTGCTACCG GGTCACTGAG 
GGCCGAGGGA TCTTTGCCAG TGGAAGTCCT TTTAAGAGCG TGACTCTAGA AGATGGCAGG 
ACCTTCATTC CTGGGCAAGG AAACAATGCC TATGTGTTCC CTGGAGTGGC TCTGGGAGTC 
ATTGCTGGCG GGATCCGGCA CATCCCAGAT GAGATCTTCC TCCTGACGGC AGAGCAAATT 
GCCCAAGAGG TCTCTGAGCA GCATTTGTCC CAGGGGAGAT TGTACCCACC ACTCAGCACC 
ATCCGAGATG TGTCTCTGAG AATCGCCATC AAAGTCCTCG ACTTCGCGTA CAAACACAAC 
CTGGCCTCCT ACTACCCGGA GCCCAAGGAC AAGGAGGCTT TTGTAAGATC CCTGGTCTAC 
ACTCCAGATT ATGACTCCTT TACACTGGAC AGCTATACTT GGCCCAAGGA AGCCATGAAT 
GTTCAAACAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ME3 ( XM_022407544.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022263252.1 Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X2, mRNA.

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CloneID	OCe295908
Clone ID Related Accession (Same CDS sequence)	XM_022407544.1
Accession Version	XM_022407544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1653bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NADP-dependent malic enzyme, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006603.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGAGCTGC TCAGAGACCC TCTGTACATT GGGCTGAAAC ACCAGCGCGT GCGTGGGAAG 
GAGTACGATG ACCTGCTGGA CGAGTTCATG CAGGCTGTGA CAGACAAGTT TGGAATAAAT 
TGCCTCATCC AATTTGAAGA CTTTGCCAAT GCCAATGCCT TCCGTCTGCT CAACAAATAC 
CGCAACAAGT ACTGCATGTT CAATGATGAC ATCCAAGGCA CAGCCTCCGT CGCTGTGGCA 
GGGATCCTGG CTGCTCTACG AATCACTAAG AACAAGCTCT CCAATCATGT GTTTGTTTTC 
CAAGGTGCAG GTGAGGCAGC CATGGGCATC GCACACCTCC TTGTCATGGC CCTGGAAAAA 
GAAGGTGTAC CCAAGGCGGA GGCTACAAGG AAGATCTGGA TGGTGGACTC CAAGGGACTC 
ATTGTCAAGG GGAGGAGCCA CCTAAACCAT GAGAAGGAGA TGTTTGCCCA GGACCATCCT 
GAAGTGAAGA GCCTGGAGGA GGTGGTGAGG CTGGTGAAGC CCACGGCCAT CATAGGTGTT 
GCTGCCATCG CAGGAGCCTT CACGGAGCAG ATTCTGAGGG ACATGGCCTC CTTCCACGAG 
CGCCCTATCA TCTTTGCCCT GAGCAACCCC ACCAGCAAGG CCGAGTGCAC GGCTGAGAAG 
TGCTACCGGG TCACTGAGGG CCGAGGGATC TTTGCCAGTG GAAGTCCTTT TAAGAGCGTG 
ACTCTAGAAG ATGGCAGGAC CTTCATTCCT GGGCAAGGAA ACAATGCCTA TGTGTTCCCT 
GGAGTGGCTC TGGGAGTCAT TGCTGGCGGG ATCCGGCACA TCCCAGATGA GATCTTCCTC 
CTGACGGCAG AGCAAATTGC CCAAGAGGTC TCTGAGCAGC ATTTGTCCCA GGGGAGATTG 
TACCCACCAC TCAGCACCAT CCGAGATGTG TCTCTGAGAA TCGCCATCAA AGTCCTCGAC 
TTCGCGTACA AACACAACCT GGCCTCCTAC TACCCGGAGC CCAAGGACAA GGAGGCTTTT 
GTAAGATCCC TGGTCTACAC TCCAGATTAT GACTCCTTTA CACTGGACAG CTATACTTGG 
CCCAAGGAAG CCATGAATGT TCAAACAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022263252.1
CDS	275..1927
Translation	MGAALGRGARPGLLRGKAWGAVPRWTPSAPGRGCRSRAGPARPVPLKKRGYDVTRNPHLNKGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQQYGLTFRRPRGLFITIHDKGHLATMLNSWPEDDIKELLRDPLYIGLKHQRVRGKEYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKAEATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVKSLEEVVRLVKPTAIIGVAAIAGAFTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGRTFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLSTIRDVSLRIAIKVLDFAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMNVQTV

Target ORF information:

RefSeq Version	XM_022407544.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris malic enzyme 3 (ME3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022407544.1

ORF Insert Sequence:

ATGGGCGCCG CGCTGGGCAG GGGCGCGCGG CCGGGGCTCC TGCGGGGTAA GGCTTGGGGC 
GCCGTCCCGC GCTGGACGCC TTCGGCTCCG GGCCGCGGCT GCCGTTCCAG GGCCGGCCCG 
GCCCGCCCCG TGCCCCTGAA GAAGCGCGGG TACGACGTCA CCAGGAACCC GCATCTCAAC 
AAGGGGATGG CCTTTACACT TGAAGAAAGG CTGCAGCTTG GAATCCATGG CCTGATCCCT 
CCCTGCTTCC TGAGCCAGGA CGTCCAGCTT CTCCGGATCA TGAGATACTA TGAGCGGCAG 
CAGAGTGACC TGGACAAGTA CATCATTCTC ATGACGCTCC AAGACCGGAA TGAGAAGCTC 
TTCTACCGAG TGCTGACATC AGATGTGGAG AAATTCATGC CCATCGTATA CACTCCCACT 
GTGGGGCTGG CCTGTCAGCA GTATGGCCTG ACCTTCCGCA GGCCCCGTGG GCTGTTCATC 
ACCATTCATG ACAAAGGCCA TCTTGCAACA ATGCTGAACT CTTGGCCAGA AGACGATATT 
AAGGAGCTGC TCAGAGACCC TCTGTACATT GGGCTGAAAC ACCAGCGCGT GCGTGGGAAG 
GAGTACGATG ACCTGCTGGA CGAGTTCATG CAGGCTGTGA CAGACAAGTT TGGAATAAAT 
TGCCTCATCC AATTTGAAGA CTTTGCCAAT GCCAATGCCT TCCGTCTGCT CAACAAATAC 
CGCAACAAGT ACTGCATGTT CAATGATGAC ATCCAAGGCA CAGCCTCCGT CGCTGTGGCA 
GGGATCCTGG CTGCTCTACG AATCACTAAG AACAAGCTCT CCAATCATGT GTTTGTTTTC 
CAAGGTGCAG GTGAGGCAGC CATGGGCATC GCACACCTCC TTGTCATGGC CCTGGAAAAA 
GAAGGTGTAC CCAAGGCGGA GGCTACAAGG AAGATCTGGA TGGTGGACTC CAAGGGACTC 
ATTGTCAAGG GGAGGAGCCA CCTAAACCAT GAGAAGGAGA TGTTTGCCCA GGACCATCCT 
GAAGTGAAGA GCCTGGAGGA GGTGGTGAGG CTGGTGAAGC CCACGGCCAT CATAGGTGTT 
GCTGCCATCG CAGGAGCCTT CACGGAGCAG ATTCTGAGGG ACATGGCCTC CTTCCACGAG 
CGCCCTATCA TCTTTGCCCT GAGCAACCCC ACCAGCAAGG CCGAGTGCAC GGCTGAGAAG 
TGCTACCGGG TCACTGAGGG CCGAGGGATC TTTGCCAGTG GAAGTCCTTT TAAGAGCGTG 
ACTCTAGAAG ATGGCAGGAC CTTCATTCCT GGGCAAGGAA ACAATGCCTA TGTGTTCCCT 
GGAGTGGCTC TGGGAGTCAT TGCTGGCGGG ATCCGGCACA TCCCAGATGA GATCTTCCTC 
CTGACGGCAG AGCAAATTGC CCAAGAGGTC TCTGAGCAGC ATTTGTCCCA GGGGAGATTG 
TACCCACCAC TCAGCACCAT CCGAGATGTG TCTCTGAGAA TCGCCATCAA AGTCCTCGAC 
TTCGCGTACA AACACAACCT GGCCTCCTAC TACCCGGAGC CCAAGGACAA GGAGGCTTTT 
GTAAGATCCC TGGTCTACAC TCCAGATTAT GACTCCTTTA CACTGGACAG CTATACTTGG 
CCCAAGGAAG CCATGAATGT TCAAACAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ME3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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