NPHS1 cDNA ORF clone, Canis lupus familiaris(Dog)

The following NPHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe286452 XM_022403537.1
Latest version!
Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe286453 XM_022403540.1
Latest version!
Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe02321 XM_541685.6
Latest version!
Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe02322 XM_005616691.2
Latest version!
Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe02323 XM_005616692.3
Latest version!
Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OCe02321 XM_541685.5 Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OCe02323 XM_005616692.2 Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe286452
    Clone ID Related Accession (Same CDS sequence) XM_022403537.1
    Accession Version XM_022403537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3318bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGAG CAATCTTTTT CCTTATGGAG GTCTAGACGA
    GCCTTCTGGA GAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259245.1
    CDS295..3612
    Translation

    Target ORF information:

    RefSeq Version XM_022403537.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGAG CAATCTTTTT CCTTATGGAG GTCTAGACGA
    GCCTTCTGGA GAACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286453
    Clone ID Related Accession (Same CDS sequence) XM_022403540.1
    Accession Version XM_022403540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGGGGTC CCAGGAGAAA CTCTTCACCA CAGAGGCCAC AGCCAGATGA TGACTCTGAC 
    TCCAGAACCC CAACTTCCCA CCCCAGGGTG ACACCCCAGA GCTCAGATAA TGGGCAGCTA
    TTGGTCTGTG AGGGGTCCAG CCCAGCATTG GACACCCCTA TCCGGGTCTC GGTCACCATG
    AATGTTCTGT TCCCCCCAGG GCCCCCTGTC ATTGAATGGC CAGGCCTGGA CGAGGGGCAT
    GTGCGGGCAG GACAGAGCTT GGAGCTGCTG TGTGTGGCCC GAGGGGGGAA CCCGCCAGCC
    ACACTGCAGT GGCTGAAGAA TGGCCAGCCC ATGTCCACAG CGTGGGGCAC AGAGCACGCT
    GAGGCAGTGG CCCGAAGCAT GCTAGTGATG GTCGTGCGAC CGGAAGACCA CGGGGCGAGG
    CTCAGCTGTG AGGCCCACAA CAGCGTATCT ACAGGGATCC AGGAGCGCGG GGTCACGCTG
    CAGGTCACCT TTCCCCCTAG TGCCATTACC ATCTTGGGAT CCGCATCCCA GTCTGAGAAC
    AAGAACGTTA CGCTCTCCTG CATCTCCAAG TCCAGTCGCC CACGGGTCCT GTTGCGATGG
    TGGCTGGGTT GGCGGCAGCT GGTGCCCACA GAAGAGATGG TCGTGGATGG CCTGCATGGT
    GGCCACATCT CCATGTCCAA TTTGACGTTC CTGGTGCGAC GGGAGGACAA TGGTCTGACT
    CTCACGTGTG AGGCCTTTAG TGAGGCCTTC ACCAAGGAGA CCTTCAAGAA ATCACTCACC
    CTGAATGTGA AATATCCTGC CCAGAAGTTG TGGATAGAGG GTCCGCCAGA GGGACAACGC
    CTCCGGGCTG GGACTCGAGT GAGGCTGGTG TGTTTGGCCA TTGGAGGCAA CCCAGAACCC
    TCTCTTACTT GGCTCAAGGA CTCCCGGACG GTGACTGAAC CGCGGCCGCC CCAGGAGCCG
    CGGAGGGTGC AGCTGGGAAG TCTGGAGAAG TCAGGGAGCA CGTTCTCCCG AGAGCTTATA
    CTGATCACGG GTCCATCGGA CAACCAGGCC AAGTTCACGT GCAAGGCGGG TCAGCTGAGC
    GCATCCACGC AGCTTGTGGT GCAGTTTCCC CCAACAAACG TGACGATCTT GGCCAATGCT
    ACGGCACTGC GCCCAGGGGA TGCATTAAAC TTGACTTGCA TCAGTGTTAG CAGCAACCCG
    CCTGTCAACT TGTCGTGGGA CAAGGAGGGG GAGAGGCTGG AAAGTGTGGC CACACCGCCC
    CGTAGTGCCC CATTCAAAGG CTCTGCGGCA GCCAGGAGCG TCCTTCTGAG AATGTCATCC
    CGAGACCACG GCCACCGAGT GACCTGCCAA GCCCACAGTC CTGAGCTACG GGAAACCATG
    AGCGCTTTCT ACCGCCTCAA CGTGCTGTAT CCTCCAGAGT TCCTGGGGGA GCAGGTGCTT
    GTGGTGACCG CGGTGGAGCA GGGTGAGGCA CTGCTGCCGG TGTCTGTGTC TGCCAACCCT
    GCCCCGGAGG CCTTCAACTG GACTTTCCGT GGCTACCGTC TCAGCCCAGC TGGCGGCCCT
    CGGCATCGCA TCTTGTCTGG TGGAGCTCTG CAGCTGTGGA ATGTGACCCG TGCTGACGAT
    GGCCTCTATC AGCTGCATTG CCAGAACTCA GAGGGCACCG CCGAAACTCT AGTGCGGCTG
    GATGTACACT ATGCTCCCAC TATCCGAGCT CTCCAGGATC CTACTGAGGT GAATATTGGG
    GGTTCTGTGG ACATAGTCTG CACTGTTGAT GCCAATCCTA TCCTTCCAGA GATGTTCAGC
    TGGGAGAGGC TGGGAGAAGA GGAGGAGGAC CAGAGCCTGG ATGACATGGA GAAGATATCA
    AAAGGATCGA CAGGGCGTCT TCGAATTCAC CATGCCAAAC TGGCCCAGGC TGGTGCTTAC
    CAGTGCATCG TGGATAATGG AGTGGCACCT CCAGCCCGAG GGTTGGTCCG ACTTGTTGTC
    AGATTCGCCC CCCAGGTGGA GCACCCCACT CCCCTAAACA AGGTGGCTGC AGCTGGAGAC
    AGTACAAGTT CTGCCACTCT CCACTGTCGT GCCCGAGGCG TCCCCAACAT CGTCTTCACT
    TGGGCCAAAA ATGGAGTCCC TCTGGATCTC GAGGATCCCA GGTACACAGA GCACACATAC
    CACCAGGGTG GGGTCCACAG CAGTCTCCTG ACCATTGCCA ACGTGTCTGC AGCCCAGGAT
    TATGCCCTCT TCACGTGCAC AGCCACCAAC CCCCTTGGCT CAGACCACAC CAACATTCAA
    CTCGTCAGCA TTAGCCGCCC GGATCCCCCA ACAGGATTAA AGGTTGTAAG CATGACCCCA
    TATTCGGTGG GGTTGGAATG GAAGCCTGGC TTTGATGGGG GTTTGCCACA GAAGTTCCAA
    ATCAGATATG AGGCACTTGG GACTCCAGGG TTCCTCTACA TGGACGTCCT ACCGCCTGAT
    GCCACCACCT TCACACTAAC TGGGCTACAG CCTTCTACAC GATACAGGGT CTGGCTGCTA
    GCCAGCAATG CCCTGGGGGA CAGCGGACTA ACTGACAAGG GGTCCCAGAT CCCTATTACC
    ACCCCAGGTC TAGACGAGCC TTCTGGAGAA CCTGACTACC AGCTGCCCAC AGAGCAGCCT
    GTAGGACCCT CAGGGCTGCC TCTACTGCCT GTGCTGTTTG CTGTTGGGGG TCTTTTGCTG
    CTTTCTAATA TCTCCTGTGT TGGAGGATTC CTCTGGCAGC GGAGACTGAG GCGTCTTGCT
    GAAGGCATCT CGGAGAAGAC AGAGACAGGG TCGGAGGAGG ACCGAGTCAG GAATGAATAT
    GAGGACAGCC GGTGGACTGG AGACCAGGAC ACTCGAAGCT CCACAGTTAG CACAACAGAT
    GTGGAACCAT ATTACCACTC CATGAAGAAC TTCAGCCCCC AGCTGCCACC AACACTGGAG
    GAGATGTCTT ATCTCCGAGG TTTCACACAT ATTGAAGAGG AGAATATGGC TTTTCCTGGA
    CACCTGTATG ATGAAGTGGA AAGGCTATAT GCCCCGCCTG GGGCCTGGGG ACCCATCTAT
    GATGAAGTAC CAATGGGCCC CTCTGACCCC TCCTGGCCTG AGGAGACATA TGAGGATACA
    AGAAGAATCT ATGATCGGGT AGCAGGAAAC TTGGACCCTG TGGAAACTGA TTCTCTACCC
    TTTGAGCTGA GGGGACATCT GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259248.1
    CDS124..3330
    Translation

    Target ORF information:

    RefSeq Version XM_022403540.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCGGGGTC CCAGGAGAAA CTCTTCACCA CAGAGGCCAC AGCCAGATGA TGACTCTGAC 
    TCCAGAACCC CAACTTCCCA CCCCAGGGTG ACACCCCAGA GCTCAGATAA TGGGCAGCTA
    TTGGTCTGTG AGGGGTCCAG CCCAGCATTG GACACCCCTA TCCGGGTCTC GGTCACCATG
    AATGTTCTGT TCCCCCCAGG GCCCCCTGTC ATTGAATGGC CAGGCCTGGA CGAGGGGCAT
    GTGCGGGCAG GACAGAGCTT GGAGCTGCTG TGTGTGGCCC GAGGGGGGAA CCCGCCAGCC
    ACACTGCAGT GGCTGAAGAA TGGCCAGCCC ATGTCCACAG CGTGGGGCAC AGAGCACGCT
    GAGGCAGTGG CCCGAAGCAT GCTAGTGATG GTCGTGCGAC CGGAAGACCA CGGGGCGAGG
    CTCAGCTGTG AGGCCCACAA CAGCGTATCT ACAGGGATCC AGGAGCGCGG GGTCACGCTG
    CAGGTCACCT TTCCCCCTAG TGCCATTACC ATCTTGGGAT CCGCATCCCA GTCTGAGAAC
    AAGAACGTTA CGCTCTCCTG CATCTCCAAG TCCAGTCGCC CACGGGTCCT GTTGCGATGG
    TGGCTGGGTT GGCGGCAGCT GGTGCCCACA GAAGAGATGG TCGTGGATGG CCTGCATGGT
    GGCCACATCT CCATGTCCAA TTTGACGTTC CTGGTGCGAC GGGAGGACAA TGGTCTGACT
    CTCACGTGTG AGGCCTTTAG TGAGGCCTTC ACCAAGGAGA CCTTCAAGAA ATCACTCACC
    CTGAATGTGA AATATCCTGC CCAGAAGTTG TGGATAGAGG GTCCGCCAGA GGGACAACGC
    CTCCGGGCTG GGACTCGAGT GAGGCTGGTG TGTTTGGCCA TTGGAGGCAA CCCAGAACCC
    TCTCTTACTT GGCTCAAGGA CTCCCGGACG GTGACTGAAC CGCGGCCGCC CCAGGAGCCG
    CGGAGGGTGC AGCTGGGAAG TCTGGAGAAG TCAGGGAGCA CGTTCTCCCG AGAGCTTATA
    CTGATCACGG GTCCATCGGA CAACCAGGCC AAGTTCACGT GCAAGGCGGG TCAGCTGAGC
    GCATCCACGC AGCTTGTGGT GCAGTTTCCC CCAACAAACG TGACGATCTT GGCCAATGCT
    ACGGCACTGC GCCCAGGGGA TGCATTAAAC TTGACTTGCA TCAGTGTTAG CAGCAACCCG
    CCTGTCAACT TGTCGTGGGA CAAGGAGGGG GAGAGGCTGG AAAGTGTGGC CACACCGCCC
    CGTAGTGCCC CATTCAAAGG CTCTGCGGCA GCCAGGAGCG TCCTTCTGAG AATGTCATCC
    CGAGACCACG GCCACCGAGT GACCTGCCAA GCCCACAGTC CTGAGCTACG GGAAACCATG
    AGCGCTTTCT ACCGCCTCAA CGTGCTGTAT CCTCCAGAGT TCCTGGGGGA GCAGGTGCTT
    GTGGTGACCG CGGTGGAGCA GGGTGAGGCA CTGCTGCCGG TGTCTGTGTC TGCCAACCCT
    GCCCCGGAGG CCTTCAACTG GACTTTCCGT GGCTACCGTC TCAGCCCAGC TGGCGGCCCT
    CGGCATCGCA TCTTGTCTGG TGGAGCTCTG CAGCTGTGGA ATGTGACCCG TGCTGACGAT
    GGCCTCTATC AGCTGCATTG CCAGAACTCA GAGGGCACCG CCGAAACTCT AGTGCGGCTG
    GATGTACACT ATGCTCCCAC TATCCGAGCT CTCCAGGATC CTACTGAGGT GAATATTGGG
    GGTTCTGTGG ACATAGTCTG CACTGTTGAT GCCAATCCTA TCCTTCCAGA GATGTTCAGC
    TGGGAGAGGC TGGGAGAAGA GGAGGAGGAC CAGAGCCTGG ATGACATGGA GAAGATATCA
    AAAGGATCGA CAGGGCGTCT TCGAATTCAC CATGCCAAAC TGGCCCAGGC TGGTGCTTAC
    CAGTGCATCG TGGATAATGG AGTGGCACCT CCAGCCCGAG GGTTGGTCCG ACTTGTTGTC
    AGATTCGCCC CCCAGGTGGA GCACCCCACT CCCCTAAACA AGGTGGCTGC AGCTGGAGAC
    AGTACAAGTT CTGCCACTCT CCACTGTCGT GCCCGAGGCG TCCCCAACAT CGTCTTCACT
    TGGGCCAAAA ATGGAGTCCC TCTGGATCTC GAGGATCCCA GGTACACAGA GCACACATAC
    CACCAGGGTG GGGTCCACAG CAGTCTCCTG ACCATTGCCA ACGTGTCTGC AGCCCAGGAT
    TATGCCCTCT TCACGTGCAC AGCCACCAAC CCCCTTGGCT CAGACCACAC CAACATTCAA
    CTCGTCAGCA TTAGCCGCCC GGATCCCCCA ACAGGATTAA AGGTTGTAAG CATGACCCCA
    TATTCGGTGG GGTTGGAATG GAAGCCTGGC TTTGATGGGG GTTTGCCACA GAAGTTCCAA
    ATCAGATATG AGGCACTTGG GACTCCAGGG TTCCTCTACA TGGACGTCCT ACCGCCTGAT
    GCCACCACCT TCACACTAAC TGGGCTACAG CCTTCTACAC GATACAGGGT CTGGCTGCTA
    GCCAGCAATG CCCTGGGGGA CAGCGGACTA ACTGACAAGG GGTCCCAGAT CCCTATTACC
    ACCCCAGGTC TAGACGAGCC TTCTGGAGAA CCTGACTACC AGCTGCCCAC AGAGCAGCCT
    GTAGGACCCT CAGGGCTGCC TCTACTGCCT GTGCTGTTTG CTGTTGGGGG TCTTTTGCTG
    CTTTCTAATA TCTCCTGTGT TGGAGGATTC CTCTGGCAGC GGAGACTGAG GCGTCTTGCT
    GAAGGCATCT CGGAGAAGAC AGAGACAGGG TCGGAGGAGG ACCGAGTCAG GAATGAATAT
    GAGGACAGCC GGTGGACTGG AGACCAGGAC ACTCGAAGCT CCACAGTTAG CACAACAGAT
    GTGGAACCAT ATTACCACTC CATGAAGAAC TTCAGCCCCC AGCTGCCACC AACACTGGAG
    GAGATGTCTT ATCTCCGAGG TTTCACACAT ATTGAAGAGG AGAATATGGC TTTTCCTGGA
    CACCTGTATG ATGAAGTGGA AAGGCTATAT GCCCCGCCTG GGGCCTGGGG ACCCATCTAT
    GATGAAGTAC CAATGGGCCC CTCTGACCCC TCCTGGCCTG AGGAGACATA TGAGGATACA
    AGAAGAATCT ATGATCGGGT AGCAGGAAAC TTGGACCCTG TGGAAACTGA TTCTCTACCC
    TTTGAGCTGA GGGGACATCT GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02321
    Clone ID Related Accession (Same CDS sequence) XM_541685.5 , XM_541685.6
    Accession Version XM_541685.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541685.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC
    CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT
    GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG
    CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG
    GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC
    TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC
    CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG
    GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT
    GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT
    GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT
    GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541685.3
    CDS2..3889
    Translation

    Target ORF information:

    RefSeq Version XM_541685.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541685.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC
    CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT
    GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG
    CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG
    GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC
    TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC
    CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG
    GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT
    GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT
    GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT
    GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02321
    Clone ID Related Accession (Same CDS sequence) XM_541685.5 , XM_541685.6
    Accession Version XM_541685.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_541685.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC
    CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT
    GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG
    CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG
    GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC
    TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC
    CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG
    GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT
    GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT
    GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT
    GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_541685.3
    CDS294..4181
    Translation

    Target ORF information:

    RefSeq Version XM_541685.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_541685.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC
    CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT
    GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG
    CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG
    GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC
    TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC
    CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG
    GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT
    GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGGGCC CCTCTGACCC CTCCTGGCCT
    GAGGAGACAT ATGAGGATAC AAGAAGAATC TATGATCGGG TAGCAGGAAA CTTGGACCCT
    GTGGAAACTG ATTCTCTACC CTTTGAGCTG AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02322
    Clone ID Related Accession (Same CDS sequence) XM_005616691.2
    Accession Version XM_005616691.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616691.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC
    CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT
    GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG
    CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG
    GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC
    TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC
    CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG
    GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT
    GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGACAT ATGAGGATAC AAGAAGAATC
    TATGATCGGG TAGCAGGAAA CTTGGACCCT GTGGAAACTG ATTCTCTACC CTTTGAGCTG
    AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616748.1
    CDS2..3859
    Translation

    Target ORF information:

    RefSeq Version XM_005616691.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616691.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG GTCAGCTGAG CGCATCCACG CAGCTTGTGG TGCAGTTTCC CCCAACAAAC
    GTGACGATCT TGGCCAATGC TACGGCACTG CGCCCAGGGG ATGCATTAAA CTTGACTTGC
    ATCAGTGTTA GCAGCAACCC GCCTGTCAAC TTGTCGTGGG ACAAGGAGGG GGAGAGGCTG
    GAAAGTGTGG CCACACCGCC CCGTAGTGCC CCATTCAAAG GCTCTGCGGC AGCCAGGAGC
    GTCCTTCTGA GAATGTCATC CCGAGACCAC GGCCACCGAG TGACCTGCCA AGCCCACAGT
    CCTGAGCTAC GGGAAACCAT GAGCGCTTTC TACCGCCTCA ACGTGCTGTA TCCTCCAGAG
    TTCCTGGGGG AGCAGGTGCT TGTGGTGACC GCGGTGGAGC AGGGTGAGGC ACTGCTGCCG
    GTGTCTGTGT CTGCCAACCC TGCCCCGGAG GCCTTCAACT GGACTTTCCG TGGCTACCGT
    CTCAGCCCAG CTGGCGGCCC TCGGCATCGC ATCTTGTCTG GTGGAGCTCT GCAGCTGTGG
    AATGTGACCC GTGCTGACGA TGGCCTCTAT CAGCTGCATT GCCAGAACTC AGAGGGCACC
    GCCGAAACTC TAGTGCGGCT GGATGTACAC TATGCTCCCA CTATCCGAGC TCTCCAGGAT
    CCTACTGAGG TGAATATTGG GGGTTCTGTG GACATAGTCT GCACTGTTGA TGCCAATCCT
    ATCCTTCCAG AGATGTTCAG CTGGGAGAGG CTGGGAGAAG AGGAGGAGGA CCAGAGCCTG
    GATGACATGG AGAAGATATC AAAAGGATCG ACAGGGCGTC TTCGAATTCA CCATGCCAAA
    CTGGCCCAGG CTGGTGCTTA CCAGTGCATC GTGGATAATG GAGTGGCACC TCCAGCCCGA
    GGGTTGGTCC GACTTGTTGT CAGATTCGCC CCCCAGGTGG AGCACCCCAC TCCCCTAAAC
    AAGGTGGCTG CAGCTGGAGA CAGTACAAGT TCTGCCACTC TCCACTGTCG TGCCCGAGGC
    GTCCCCAACA TCGTCTTCAC TTGGGCCAAA AATGGAGTCC CTCTGGATCT CGAGGATCCC
    AGGTACACAG AGCACACATA CCACCAGGGT GGGGTCCACA GCAGTCTCCT GACCATTGCC
    AACGTGTCTG CAGCCCAGGA TTATGCCCTC TTCACGTGCA CAGCCACCAA CCCCCTTGGC
    TCAGACCACA CCAACATTCA ACTCGTCAGC ATTAGCCGCC CGGATCCCCC AACAGGATTA
    AAGGTTGTAA GCATGACCCC ATATTCGGTG GGGTTGGAAT GGAAGCCTGG CTTTGATGGG
    GGTTTGCCAC AGAAGTTCCA AATCAGATAT GAGGCACTTG GGACTCCAGG GTTCCTCTAC
    ATGGACGTCC TACCGCCTGA TGCCACCACC TTCACACTAA CTGGGCTACA GCCTTCTACA
    CGATACAGGG TCTGGCTGCT AGCCAGCAAT GCCCTGGGGG ACAGCGGACT AACTGACAAG
    GGGTCCCAGA TCCCTATTAC CACCCCAGGT CTAGACGAGC CTTCTGGAGA ACCTGACTAC
    CAGCTGCCCA CAGAGCAGCC TGTAGGACCC TCAGGGCTGC CTCTACTGCC TGTGCTGTTT
    GCTGTTGGGG GTCTTTTGCT GCTTTCTAAT ATCTCCTGTG TTGGAGGATT CCTCTGGCAG
    CGGAGACTGA GGCGTCTTGC TGAAGGCATC TCGGAGAAGA CAGAGACAGG GTCGGAGGAG
    GACCGAGTCA GGAATGAATA TGAGGACAGC CGGTGGACTG GAGACCAGGA CACTCGAAGC
    TCCACAGTTA GCACAACAGA TGTGGAACCA TATTACCACT CCATGAAGAA CTTCAGCCCC
    CAGCTGCCAC CAACACTGGA GGAGATGTCT TATCTCCGAG GTTTCACACA TATTGAAGAG
    GAGAATATGG CTTTTCCTGG ACACCTGTAT GATGAAGTGG AAAGGCTATA TGCCCCGCCT
    GGGGCCTGGG GACCCATCTA TGATGAAGTA CCAATGACAT ATGAGGATAC AAGAAGAATC
    TATGATCGGG TAGCAGGAAA CTTGGACCCT GTGGAAACTG ATTCTCTACC CTTTGAGCTG
    AGGGGACATC TGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02323
    Clone ID Related Accession (Same CDS sequence) XM_005616692.3 , XM_005616692.2
    Accession Version XM_005616692.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product nephrin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005616692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA
    GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG
    TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC
    AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC
    CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC
    CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG
    GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC
    AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG
    TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG
    CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT
    CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA
    GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA
    GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG
    CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT
    AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG
    GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC
    ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA
    GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC
    CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG
    TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC
    CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG
    GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA
    CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA
    CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG
    GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC
    GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG
    CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC
    TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG
    AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG
    ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC
    CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC
    CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA
    GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG
    GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT
    CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA
    CATCTGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616749.1
    CDS295..4146
    Translation

    Target ORF information:

    RefSeq Version XM_005616692.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NPHS1, nephrin (NPHS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616692.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA
    GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG
    TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC
    AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC
    CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC
    CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG
    GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC
    AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG
    TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG
    CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT
    CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA
    GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA
    GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG
    CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT
    AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG
    GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC
    ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA
    GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC
    CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG
    TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC
    CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG
    GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA
    CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA
    CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG
    GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC
    GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG
    CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC
    TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG
    AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG
    ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC
    CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC
    CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA
    GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG
    GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT
    CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA
    CATCTGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe02323
    Clone ID Related Accession (Same CDS sequence) XM_005616692.3 , XM_005616692.2
    Accession Version XM_005616692.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product nephrin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005616692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA
    GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG
    TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC
    AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC
    CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC
    CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG
    GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC
    AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG
    TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG
    CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT
    CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA
    GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA
    GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG
    CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT
    AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG
    GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC
    ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA
    GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC
    CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG
    TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC
    CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG
    GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA
    CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA
    CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG
    GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC
    GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG
    CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC
    TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG
    AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG
    ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC
    CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC
    CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA
    GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG
    GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT
    CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA
    CATCTGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005616749.1
    CDS2..3853
    Translation

    Target ORF information:

    RefSeq Version XM_005616692.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris nephrosis 1, congenital, Finnish type (nephrin) (NPHS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005616692.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGAAAGAG AGGAAAACAG AGAAATAGAA GGAGACGAAT GGATGAAGAG AGACAATAAG 
    AAAAGCCAGA CACATGGAGA GAGAGATCCA GCTAAAGACA GAACCAGAGA CAGGAGGCAG
    GCAGTGGGCA CTGTGGGAGC CACAGCCAGA GGACCCAGGA TGGCCCTGAG GACCCCCAGC
    AGGGCTCCCC TGCTCCTCAT GGGGCTGCTG ACCACAGGCC TGGCCCAGTT GCCGCTCTCT
    GCCTCGGCCC CTCGAGGCTT CTGGGCTCTG CCAGAAAACT TGACGGTGGT GGAGGGAGCC
    ACTGCTGAGC TGCAGTGTGG GGTCCACGCC CCTGGCAGTG TGGTACAATG GGCCAAAGAT
    GGGCTACTCC TGGGCCCTGA TCCTAGGATC CCCCGCTTCC CGAGGTACCG CCTGGAAGGA
    GACCCGAGTA GAGGTGAATT CCACCTGCAT ATTGAAGCGT GTGACCTGAG CGACGATGCA
    GAGTATGAGT GTCAGGTCGG CCGCTCGGAG ACAGGCCCTG AGCTCGTGTC TCCCAGAGTG
    ATCCTCTCCG TCCTGGTGCC CCCCAAAGTG CTTCAGCTGA CCCCTGAGGC AGGGAGCACA
    GTCACGTGGG TAGCTGGGCA GGAGTACGTG GTCACCTGTG TGTCTGGGGA CGCGAAGCCG
    GCACCCGACA TCACCTTCTT CCAGAGTGGA CAAACAGTTT CTGACATCTC TGCCAATGTG
    AATGCGGGGT CCCAGGAGAA ACTCTTCACC ACAGAGGCCA CAGCCAGGGT GACACCCCAG
    AGCTCAGATA ATGGGCAGCT ATTGGTCTGT GAGGGGTCCA GCCCAGCATT GGACACCCCT
    ATCCGGGTCT CGGTCACCAT GAATGTTCTG TTCCCCCCAG GGCCCCCTGT CATTGAATGG
    CCAGGCCTGG ACGAGGGGCA TGTGCGGGCA GGACAGAGCT TGGAGCTGCT GTGTGTGGCC
    CGAGGGGGGA ACCCGCCAGC CACACTGCAG TGGCTGAAGA ATGGCCAGCC CATGTCCACA
    GCGTGGGGCA CAGAGCACGC TGAGGCAGTG GCCCGAAGCA TGCTAGTGAT GGTCGTGCGA
    CCGGAAGACC ACGGGGCGAG GCTCAGCTGT GAGGCCCACA ACAGCGTATC TACAGGGATC
    CAGGAGCGCG GGGTCACGCT GCAGGTCACC TTTCCCCCTA GTGCCATTAC CATCTTGGGA
    TCCGCATCCC AGTCTGAGAA CAAGAACGTT ACGCTCTCCT GCATCTCCAA GTCCAGTCGC
    CCACGGGTCC TGTTGCGATG GTGGCTGGGT TGGCGGCAGC TGGTGCCCAC AGAAGAGATG
    GTCGTGGATG GCCTGCATGG TGGCCACATC TCCATGTCCA ATTTGACGTT CCTGGTGCGA
    CGGGAGGACA ATGGTCTGAC TCTCACGTGT GAGGCCTTTA GTGAGGCCTT CACCAAGGAG
    ACCTTCAAGA AATCACTCAC CCTGAATGTG AAATATCCTG CCCAGAAGTT GTGGATAGAG
    GGTCCGCCAG AGGGACAACG CCTCCGGGCT GGGACTCGAG TGAGGCTGGT GTGTTTGGCC
    ATTGGAGGCA ACCCAGAACC CTCTCTTACT TGGCTCAAGG ACTCCCGGAC GGTGACTGAA
    CCGCGGCCGC CCCAGGAGCC GCGGAGGGTG CAGCTGGGAA GTCTGGAGAA GTCAGGGAGC
    ACGTTCTCCC GAGAGCTTAT ACTGATCACG GGTCCATCGG ACAACCAGGC CAAGTTCACG
    TGCAAGGCGG TTCCCCCAAC AAACGTGACG ATCTTGGCCA ATGCTACGGC ACTGCGCCCA
    GGGGATGCAT TAAACTTGAC TTGCATCAGT GTTAGCAGCA ACCCGCCTGT CAACTTGTCG
    TGGGACAAGG AGGGGGAGAG GCTGGAAAGT GTGGCCACAC CGCCCCGTAG TGCCCCATTC
    AAAGGCTCTG CGGCAGCCAG GAGCGTCCTT CTGAGAATGT CATCCCGAGA CCACGGCCAC
    CGAGTGACCT GCCAAGCCCA CAGTCCTGAG CTACGGGAAA CCATGAGCGC TTTCTACCGC
    CTCAACGTGC TGTATCCTCC AGAGTTCCTG GGGGAGCAGG TGCTTGTGGT GACCGCGGTG
    GAGCAGGGTG AGGCACTGCT GCCGGTGTCT GTGTCTGCCA ACCCTGCCCC GGAGGCCTTC
    AACTGGACTT TCCGTGGCTA CCGTCTCAGC CCAGCTGGCG GCCCTCGGCA TCGCATCTTG
    TCTGGTGGAG CTCTGCAGCT GTGGAATGTG ACCCGTGCTG ACGATGGCCT CTATCAGCTG
    CATTGCCAGA ACTCAGAGGG CACCGCCGAA ACTCTAGTGC GGCTGGATGT ACACTATGCT
    CCCACTATCC GAGCTCTCCA GGATCCTACT GAGGTGAATA TTGGGGGTTC TGTGGACATA
    GTCTGCACTG TTGATGCCAA TCCTATCCTT CCAGAGATGT TCAGCTGGGA GAGGCTGGGA
    GAAGAGGAGG AGGACCAGAG CCTGGATGAC ATGGAGAAGA TATCAAAAGG ATCGACAGGG
    CGTCTTCGAA TTCACCATGC CAAACTGGCC CAGGCTGGTG CTTACCAGTG CATCGTGGAT
    AATGGAGTGG CACCTCCAGC CCGAGGGTTG GTCCGACTTG TTGTCAGATT CGCCCCCCAG
    GTGGAGCACC CCACTCCCCT AAACAAGGTG GCTGCAGCTG GAGACAGTAC AAGTTCTGCC
    ACTCTCCACT GTCGTGCCCG AGGCGTCCCC AACATCGTCT TCACTTGGGC CAAAAATGGA
    GTCCCTCTGG ATCTCGAGGA TCCCAGGTAC ACAGAGCACA CATACCACCA GGGTGGGGTC
    CACAGCAGTC TCCTGACCAT TGCCAACGTG TCTGCAGCCC AGGATTATGC CCTCTTCACG
    TGCACAGCCA CCAACCCCCT TGGCTCAGAC CACACCAACA TTCAACTCGT CAGCATTAGC
    CGCCCGGATC CCCCAACAGG ATTAAAGGTT GTAAGCATGA CCCCATATTC GGTGGGGTTG
    GAATGGAAGC CTGGCTTTGA TGGGGGTTTG CCACAGAAGT TCCAAATCAG ATATGAGGCA
    CTTGGGACTC CAGGGTTCCT CTACATGGAC GTCCTACCGC CTGATGCCAC CACCTTCACA
    CTAACTGGGC TACAGCCTTC TACACGATAC AGGGTCTGGC TGCTAGCCAG CAATGCCCTG
    GGGGACAGCG GACTAACTGA CAAGGGGTCC CAGATCCCTA TTACCACCCC AGGTCTAGAC
    GAGCCTTCTG GAGAACCTGA CTACCAGCTG CCCACAGAGC AGCCTGTAGG ACCCTCAGGG
    CTGCCTCTAC TGCCTGTGCT GTTTGCTGTT GGGGGTCTTT TGCTGCTTTC TAATATCTCC
    TGTGTTGGAG GATTCCTCTG GCAGCGGAGA CTGAGGCGTC TTGCTGAAGG CATCTCGGAG
    AAGACAGAGA CAGGGTCGGA GGAGGACCGA GTCAGGAATG AATATGAGGA CAGCCGGTGG
    ACTGGAGACC AGGACACTCG AAGCTCCACA GTTAGCACAA CAGATGTGGA ACCATATTAC
    CACTCCATGA AGAACTTCAG CCCCCAGCTG CCACCAACAC TGGAGGAGAT GTCTTATCTC
    CGAGGTTTCA CACATATTGA AGAGGAGAAT ATGGCTTTTC CTGGACACCT GTATGATGAA
    GTGGAAAGGC TATATGCCCC GCCTGGGGCC TGGGGACCCA TCTATGATGA AGTACCAATG
    GGCCCCTCTG ACCCCTCCTG GCCTGAGGAG ACATATGAGG ATACAAGAAG AATCTATGAT
    CGGGTAGCAG GAAACTTGGA CCCTGTGGAA ACTGATTCTC TACCCTTTGA GCTGAGGGGA
    CATCTGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.