PRUNE2 cDNA ORF clone, Canis lupus familiaris(Dog)

The following PRUNE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRUNE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe01322 XM_005615830.3
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01323 XM_005615831.3
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01324 XM_005615832.3
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01325 XM_005615833.2
Latest version!
Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01327 XM_005615835.3
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe286042 XM_022420968.1
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe286043 XM_022420970.1
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe286044 XM_022420979.1
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe286045 XM_022420980.1
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe286046 XM_022420984.1
Latest version!
Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OCe01323 XM_005615831.2 Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01327 XM_005615835.2 Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01322 XM_005615830.2 Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe01324 XM_005615832.2 Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe01322
    Clone ID Related Accession (Same CDS sequence) XM_005615830.3 , XM_005615830.2
    Accession Version XM_005615830.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615830.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCCATGGAG
    AAGGACATTG ACCTGAAGCT AAAAGGAAAG CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615887.1
    CDS174..9389
    Translation

    Target ORF information:

    RefSeq Version XM_005615830.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615830.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCCATGGAG
    AAGGACATTG ACCTGAAGCT AAAAGGAAAG CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01322
    Clone ID Related Accession (Same CDS sequence) XM_005615830.3 , XM_005615830.2
    Accession Version XM_005615830.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein prune homolog 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615830.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
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    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCCATGGAG
    AAGGACATTG ACCTGAAGCT AAAAGGAAAG CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615887.1
    CDS164..9379
    Translation

    Target ORF information:

    RefSeq Version XM_005615830.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615830.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAAG CAGCTAAAAC TAGCTGCCTT TACAATGATC CAGAAATGTC TTCCATGGAG
    AAGGACATTG ACCTGAAGCT AAAAGGAAAG CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01323
    Clone ID Related Accession (Same CDS sequence) XM_005615831.2 , XM_005615831.3
    Accession Version XM_005615831.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein prune homolog 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC CATGGAGAAG
    GACATTGACC TGAAGCTAAA AGGAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615888.1
    CDS164..9376
    Translation

    Target ORF information:

    RefSeq Version XM_005615831.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615831.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC CATGGAGAAG
    GACATTGACC TGAAGCTAAA AGGAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01323
    Clone ID Related Accession (Same CDS sequence) XM_005615831.2 , XM_005615831.3
    Accession Version XM_005615831.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9213bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9001
    9061
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    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC CATGGAGAAG
    GACATTGACC TGAAGCTAAA AGGAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615888.1
    CDS174..9386
    Translation

    Target ORF information:

    RefSeq Version XM_005615831.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615831.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGAACTAG CTGCCTTTAC AATGATCCAG AAATGTCTTC CATGGAGAAG
    GACATTGACC TGAAGCTAAA AGGAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01324
    Clone ID Related Accession (Same CDS sequence) XM_005615832.3 , XM_005615832.2
    Accession Version XM_005615832.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615832.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAA
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCCATGGA GAAGGACATT
    GACCTGAAGC TAAAAGGAAA GCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615889.1
    CDS174..9380
    Translation

    Target ORF information:

    RefSeq Version XM_005615832.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615832.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAA
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCCATGGA GAAGGACATT
    GACCTGAAGC TAAAAGGAAA GCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01324
    Clone ID Related Accession (Same CDS sequence) XM_005615832.3 , XM_005615832.2
    Accession Version XM_005615832.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein prune homolog 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615832.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
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    1621
    1681
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    1801
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    1981
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    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAA
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCCATGGA GAAGGACATT
    GACCTGAAGC TAAAAGGAAA GCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615889.1
    CDS164..9370
    Translation

    Target ORF information:

    RefSeq Version XM_005615832.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615832.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAA CTGGATGAAG AACTGAGGGA AGCATCAGAA
    GCAGCTAAAA CTAGCTGCCT TTACAATGAT CCAGAAATGT CTTCCATGGA GAAGGACATT
    GACCTGAAGC TAAAAGGAAA GCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01325
    Clone ID Related Accession (Same CDS sequence) XM_005615833.2
    Accession Version XM_005615833.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein prune homolog 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615833.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CCATGGAGAA GGACATTGAC
    CTGAAGCTAA AAGGAAAGCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615890.1
    CDS164..9367
    Translation

    Target ORF information:

    RefSeq Version XM_005615833.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615833.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGAACTA GCTGCCTTTA CAATGATCCA GAAATGTCTT CCATGGAGAA GGACATTGAC
    CTGAAGCTAA AAGGAAAGCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01327
    Clone ID Related Accession (Same CDS sequence) XM_005615835.2 , XM_005615835.3
    Accession Version XM_005615835.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product protein prune homolog 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAACA TTGACCTGAA GCTAAAAGGA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615892.1
    CDS164..9292
    Translation

    Target ORF information:

    RefSeq Version XM_005615835.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris prune homolog 2 (Drosophila) (PRUNE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615835.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAACA TTGACCTGAA GCTAAAAGGA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe01327
    Clone ID Related Accession (Same CDS sequence) XM_005615835.2 , XM_005615835.3
    Accession Version XM_005615835.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAACA TTGACCTGAA GCTAAAAGGA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615892.1
    CDS174..9302
    Translation

    Target ORF information:

    RefSeq Version XM_005615835.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615835.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAACA TTGACCTGAA GCTAAAAGGA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286042
    Clone ID Related Accession (Same CDS sequence) XM_022420968.1
    Accession Version XM_022420968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1501
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    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAAGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCC ATGGAGAAGG ACATTGACCT GAAGCTAAAA
    GGAAAGCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276676.1
    CDS174..9425
    Translation

    Target ORF information:

    RefSeq Version XM_022420968.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAAGCAGC TAAAACTAGC
    TGCCTTTACA ATGATCCAGA AATGTCTTCC ATGGAGAAGG ACATTGACCT GAAGCTAAAA
    GGAAAGCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286043
    Clone ID Related Accession (Same CDS sequence) XM_022420970.1
    Accession Version XM_022420970.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGACTGG ATGAAGAACT GAGGGAAGCA TCAGAAGCAG CTAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC CATGGAGAAG GACATTGACC TGAAGCTAAA AGGAAAGCCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276678.1
    CDS174..9416
    Translation

    Target ORF information:

    RefSeq Version XM_022420970.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGC TACTATGGGG ACGGCCTAAA TGCCATCATT
    GTGTTTGCTG CCTGTTTTCT GCCAGACAGC AGTCGGGCGG ATTACCACTA TGTCATGGAA
    AATCTTTTCC TATATGTAAT AAGTACTTTA GAGCTGATGG TAGCTGAGGA CTATATGATT
    GTGTACTTGA ACGGAGCAAC CCCGAGACGG AAGATGCCAG GGCTGGGCTG GATGAAGAAA
    TGCTATCAGA TGATTGACAG ACGGTTGCGA AAGAATTTGA AATCATTCAT CATTGTTCAT
    CCATCTTGGT TCATCAGGAC AATCCTTGCA GTGACACGAC CTTTTATAAG TTCAAAATTC
    AGCAGTAAAA TTAAATATGT CAATAGCTTA TCAGAACTCA GTGGGCTGAT CCCAATGGAT
    TGTATCCATA TTCCAGAGAG CATCATCAAG TACGATGAAG AGAGATCTTA TAAGAGAAGT
    GTGAGACTGG ATGAAGAACT GAGGGAAGCA TCAGAAGCAG CTAAAACTAG CTGCCTTTAC
    AATGATCCAG AAATGTCTTC CATGGAGAAG GACATTGACC TGAAGCTAAA AGGAAAGCCC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286044
    Clone ID Related Accession (Same CDS sequence) XM_022420979.1
    Accession Version XM_022420979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    9001
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    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAAGCAGC TAACATTGAC
    CTGAAGCTAA AAGGAAAGCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276687.1
    CDS174..9377
    Translation

    Target ORF information:

    RefSeq Version XM_022420979.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGACTGGA TGAAGAACTG AGGGAAGCAT CAGAAGCAGC TAACATTGAC
    CTGAAGCTAA AAGGAAAGCC CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286045
    Clone ID Related Accession (Same CDS sequence) XM_022420980.1
    Accession Version XM_022420980.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9168bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAAG CAGCTAACAT TGACCTGAAG CTAAAAGGAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276688.1
    CDS174..9341
    Translation

    Target ORF information:

    RefSeq Version XM_022420980.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420980.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAAC TGGATGAAGA ACTGAGGGAA
    GCATCAGAAG CAGCTAACAT TGACCTGAAG CTAAAAGGAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe286046
    Clone ID Related Accession (Same CDS sequence) XM_022420984.1
    Accession Version XM_022420984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9165bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGCATTGA CCTGAAGCTA AAAGGAAAGC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276692.1
    CDS174..9338
    Translation

    Target ORF information:

    RefSeq Version XM_022420984.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris prune homolog 2 (PRUNE2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022420984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAACG CGCCAAATCT AAACTGAATC GAAGCAAACG CCTGGAGAAG 
    GTCCATGTGG TTATTGGACA TAAATCGTGT GACTTGGATT CTCTCATTTC TGCCTTCACA
    TACGCTTACT TTCTAGACAA GGTCAGCCCA CCCGGAGTTC TCTGTTTGCC AGTACTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCGCC GAGACACGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATTCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATGATGAA
    GGGAAGTTAT CTATAACACT TGTTGGCAGC AATGTGCTGG CAAGTGAGGA CAACAGTTTA
    GAATCAGCGG TTGTCAAAGT CATTAATCCA GTGGAGCAAA GCGATGGTGG GCTTGAGTTC
    GGAGAGTCTT CCTCTTCCCT TGTGGTGAAA GAGATTCTCC AGGAGGCTCC TGAGCTCATC
    ACTGAGCAGC TGGCTCATCT CCTCAGAGGT AGTATCCTTT TCAAGTGGAT GACCATGGAA
    TCCGAGAATA TCTCAGAGAA GCAAGAAGAA ATTCTCTCTA TCTTGGAAGA GAAATTTCCT
    AGCTTGCCTC CAAGAGAAGA CATTATCAAC GTCCTACAAG AGACCCAATT CAGTGCTCAG
    GGTTTAAGTA TTGAACAGAT GATGCTGAAA GATCTAAAGG AACTGTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGTACTGT GAGCATGACC CTCGAGAACT GTATGTTTCA CAGCAATATC
    AGCAGTGGCT TGAAAGCATT TGCAGACAAA TTCGGTTTTG ATGTTCTCAT CCTCCTGGCC
    AGCTACCTGT CGGAGGAAGA GCAGCCCCGA CGGCAGATCG CTGTGTACTC TCAGAACCTC
    GAGTTGTGCA GTCAGATTTG CTGTGAATTG GAAGAATGCC AGCACCCTTG CCTAGAGCTG
    GAGCCCTTTG AATGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTCTGGTG
    ACCTGTGATC AAGTGGTTCT TCTTGTCAAG GAAGTCATCA ATAGGAGGTG TCCAGAGATG
    GTCTCCAACA GCCGGACGTC CTCAACAGAA GCCGTGGCAG GCAGTGCCCC GCTGTCTCAG
    GGATCTTCTG GGATTATGGA GTTGTATGGT TCTGACGTAG AGCCACAGCC CAGCTCTGTG
    AATTTCATAG AAAACCCTCC AGATCTGAGC GATTCTAACC AGGCTCAGGT GGATATGAAC
    GTAGACCTTG TCAGCCCAGA TAGCGGGTTG GCCACTATTA GGAGCAGCCG TTCATCCAAG
    GAGAGTTCAG TTTTCCTCAG TGATGACAGT CCAGTAGGAG AAGGTGCAGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTATAGT CCCATCCCTG AAGGAGCAGT AGCAGAAGAA
    CATGCCCGGT CAGGAGAACA CAGTGAATCC TTTGACCTCT TCAACTTTGA TCAAGCCCCC
    ATGGTTTCTG GGCAATCTCA ACCATCTTCC CATTCTGCAG ACTACTCACC TGCAGATGAC
    TTCTTCCCCA ATAGTGATTC ATCAGAAGGA CAACCCCCTA CGAGGCCTAA AGAAATCAAT
    GGGATAGGGA TGGACTTGTC TAATTACTCA CCCAGTTCGC TTTTGTCAGG AGCTGGCAAA
    GATAACCTTG TGGAATTTGA TGAAGAGTTT GGTCAGAGAC AAGAAAGTCC CCAGAATCAC
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT GTGGGAGAGA AATCTCCTTC ACCAGAGAGG
    CTAAAAAATA TTGGAAAAAG GGTCCCACCA ACACCTATGA ATAGCTTTGT AGAAAGCTCA
    CCATCTACCG AAGAACCAGC TCTACTCTAT CCAGAAAATG TGACCCCCAA AGCAGTAGAT
    GCAGGTCACA TAGGGCCACC TCAGACCCGT GCAAGGTGCA GCAGCTGGTG GGGTGGTTTG
    GAAATTGACT CTAAAAATAT TGTAGATGCA TGGAGTTCCA GCGAACAGGA GTCTGTCTTC
    CAGAGCCCCG AGTCATGGAA AGATCATAAG CCAAGCCCAG TTGATCGGAG AGCCTCCGAT
    TCTATATTTC AACCAAAGAG TCTTGAATTC CCAAAGTCAA GTCCTTGGGA GTCTGAATTT
    GGTCAGCCAG GGCTTGACAG CAATAATATT CAAGACCAAA ATGAGGACAA TTTGCAGTTT
    CAGAATGTGC CCACAGAGAA ATCAAATTTG CCAAATTCTC CTCAGGGAAC GAATCACCTG
    ATAGAAGACT TTGCTGCCTT GTGGCATTCT GATCGTTCTC CCACAGCGAT GCCAGAACCA
    TGGGGAAACC CCACAGAAGA CGGTACTCCA GCTTCTACGG TGTCATTCCC AGCCTGGAGT
    GCATTTGGTA AAGAAGATAG CACCAAAGCT TTGACAAATA CCTGGAATCT GCACCCAACG
    AGTGGTGAGA CACCTTCTGT GAGGGACCCA AGTGAGTGGG CCATGGCAAA AAGTGGGTTT
    TCTTTTCCTT CAGAAGACAA TTCACCCAGT GAGGGAAATA ATGAAGTAGC TCCAGAAATC
    TGGGGCAGGA AGAACCACGA CCCCAGGGAT AGCATCCTTA TATCTGGCAA CCCCACCTCT
    GATCTGGATC ATGCGTGGAA TAGTTCTAAG CCATCACAGG AAGATCAGAA TGGTTTAGTG
    GATCCAAACA TTAGGGGCAA GGTGTATGAA AAGGTCGATT CCTGGAACCT TTTTGAGGAG
    GGTATCAAGA AAGGAGGGTC AGACGTCCTG GCACCTTGGG AAGATTCCTT CTTATATAAA
    TGTTCCGATT ACAGTGCATC CAACATGGGG GAAGATTCGG TGCCTTCCCC CTTAGATACC
    AACTACTCCA CGTCTGACTC TTACACTTCG CCAACATTTG CTGGAGACGA AAAAGAAACA
    GAAAACAAGC CATTTGCTGA AGAGGGAGGC TTGGAGTCAA AAGATGCTAA CTCTGCCACA
    GGGGAGACTG AGATGCCTCC TCAGTCACCG CAGCAGCCAG CTAGAAATCG AATTGGCTCA
    GATCCTGGGA ACCTAGAAAT GTGGGGTTCC CCTGATACAG AAAATAATCC TCAAATAAAT
    GTCACTCATA ACTTGGATAA AGATTTACTC GAGACAGAGC CCATAGATGA TAAGAATATC
    TCCCTGGAGG ATGACATCGG GGAAAGCAGC CTGTCCAGTT ACGACGACCC CAGCATGATG
    CAGCTGTACA ACGAAACCAA CCGGCAGCTC ACGCTTCTGC ACAGCAGCAC CAACTCTCGC
    CAGGCGGTCC CTGACAGTCT CGACCTGTGG AACAGGGTGA TTCTGGAGGA CACGCAGTCC
    ACTGCGACCA TCTCCGATGT GGACAATGAT TTGGACTGGG ATGACTGCAG TGCAGGTGTG
    GCGATCTCCA GCGAAGGTCA AGCACAAGGA TACATGGCTC AAGGTACCGA ACCCGAAACC
    CGATTTTCAG TGAGACAGCT GGAACCATGG GGTGTGGAGT ATCAGGAAGC AAGTCAGGCA
    GATTGGGAAC TCCCTGCCTC TTCTGAGCAT ATCAAGGACC CTCCTGCCAA CGAGTATCAC
    ACCCTGAATG AAAAAAGTGG GCAGCTAATT GCGAATAATA TTTGGGATTC CGTCATGAGG
    GATAATGACA TGTCTTCGTT AATGTTATCA GGCCAATCAT ATGTTACAGA TTCTGAACAA
    CACACGTCAC TTCCTGAAAC TCCCAGCTCA TCGGAAAAAA TTAAGGACAG TTATATCCCA
    GAGGTGCTAG AAGCCTCTGA AGCCAATGAG TCAGGAGCAC TTGATCCTGA TAAGCAGGAG
    ATGGGCAGAG GAACCCCACC AAATGATGCA GTGTCCTTGG CAACCAACCT CAAGAATCCA
    GGGCATTTTG CACCCTCAGA TCCATGGAAA GGTCCTAGCT ACGGACAGAG TGAAAGTGAG
    GGAAAAGCTC ATGGAGCTGC TGCTAGGGAG TGTCTCAGTG AGGAGATGCT AGATAGAGCC
    TCGGGGATTC CTGAAAAAGG GCAAAGTGAC CAGGAATGCT CTTCCCCAAT TTCATCTCAA
    CATCAAGACA TCTGCGATGA ATCAGGCAAA ATCATCTCTC TTTCAGACAC TTCCAGTCCT
    CAGACTGAGA AACCGCAGGA GGTTTCAGAG CATAAGAAAG GGTCTTACAA TCCAGATCCC
    TGTGATGTGC ACACAGAGAT GTCCACAAAC AACCCACAGG CCAAAGAGAT CTGCAAAAAG
    CACCTCATGT ATCACGGCAA TTCAGGTGAA ACCACTGAAA TTTCAGATAG CTTCAATTTA
    ACCAGAAATT TATCTTTACC TGAAATGGAT CTTGAGAAAA CTGAAGAATG TAATATTCTG
    GAGCCAGGGA GCTTGAACCA AGAGCCACCC CATGAGTGTC CCCAAAGCCT TGACATTTGG
    GATGGCTCTA TAGACAGTGA CTTGCAGGTC AATTTGCCAA GTTCTGAGAT ACCTAAGAAT
    CTTGAAGTTA AGAGTACTGA AAACAGCACT CCAGGAGCTA ATCTATCAGG AAATTATCAC
    AGAGATAGTA TTTCCAGTGA GTATTCACAT TCGAGTGCAT CGAGTCCTGG CCTAAATGAT
    TCTTCATCTG CATTGCCTTC CTGGGACCAG GGACCCAATG TGGGGCACCA GAAACAGAAT
    CAGGATGATT GGAGCAAACA GAATCACCAA GAATCTGCAC TAATTACTAC TGACAGCCAA
    GTGGGAGTAA TGACTGAAAT GAAGGATGTG GAGAAAAACA GAATAGACAG GTTGGAAAAG
    AATTTAGATC ACAAGCTTCC TAAATTTTTA GAGATTTGGA ATGACTCAGT AGATGGTGAT
    TCCTTTTCCT CGTTATCCAG CCCTGAAACA GGCAAATGTT CTGAATATTC AGATGCATAT
    CAAGAAAGAA ATCTAGTGGT TAGTCAGCAG GAGAAAAACG AGCAAGACAT TTCTAAAACT
    CTGCAGCCAG AGGATGCCAG GTTCATGTCA ACAAGCTCAG GTTCTGATGA TGAGAGCATA
    GACGATGAAG AGTCAGTGGA CAAGGAGGTC CATTTGGGCA CATGCCAAGC TGTTCAGAAT
    GAAACCAGGG CTTGGGATTC ACTGAATGAA CCTAGTAAGT TCCTGGCCAC AGCTGATCCC
    AACTCTGATG AATTTTCTAC CTATGGAGGT GGTTCAGAAC CAACAGAGAA TAAATCCACC
    CCGCTCTGTG AGAATCAACA AAGCAGCCCT GATCACTGGA ATTTCTCACC ACTAAAGGAG
    ACTGAACTCC AGATTACAGA AGTGGATAAG GAGACGAGAT CTCCAGAAAC AGGGAAGACA
    GGAGATATCC TGTGGCAGAT CTCTCCCCTA AGTGAATCAA AGAATGAGAA TCCTTCTAAA
    TTGGAAATGC TTGGCTTCTC AGCCGAGAGC ACTGAGTGGT GGAATGCCTC TCTACAAGGA
    GGGCGACCTA TTGAGAGTGC ATTTGAGAGG GAATTACCTA GCTCAGGTAG TGTGTTAGAG
    ATGAGTTCTT CAGTATGCCA AACCATGGGT CCCTGGGGAG TACCCATTCA GGGTGATAGT
    GAACCCGTGG AATCACAAGG TACCAGTCCT TTCACTGAGG AACATCAATC ACCCTTTCTG
    GATGGCAATG ACAAGAACTC CCATGAGCAA CTTTGGAACA TTCAACCAAG GCAGCCAGAC
    CCAGAAGCTG ATCAGCTTAG CCAGCTTGTA ATATTGGACC ACGTTAAAGA AAAGGATTCT
    GGAGAGGAAA CCTTCCTGTC CTCTGCTGGT CAAGAACTGA CTTCTGAAAC ACCAACCCAG
    GAGCAGCAGT GTCAGAACAC TGTGATGTCT ATCTGGGATC AGTCAGGCCC AACATTTACT
    CACACAGATG AAAATGGTTG TGTTATAGCT AATGTTTCGA CTGTTGAGTG TCAAGAAACA
    AAGTGGTGGA AAGAAGGAAA ATCATATCCC GGTGAAATAA TGAATTCAAG CGTTGCCTCA
    GAAGATTTCC CCACTGTCAA TTCTCCGACT CAGTTGATAA AGAAGTCAGA CTCCGAATGG
    GATGTCTCTA CCCCTAGTGA GGGCCCCCAA GGCACATTTG TTCCAGATAT TTTACATGGC
    AACTTCCAAG AGGGTGGGCA ACTGGCCTCA GCTTCGCCTG ATTTGTGGAT GGATGCTAAG
    CAGCCCTTCA GTGTGAAAGC AGATGGTGAG AATCCAGATA TACTGACTCA CTGTGACCAT
    GACAGCTATT CTCAGGCTTC CAACAGCCCT GATATATGTC ACGATTATGA AGCCAAGCAA
    GAGACTGAGC AGCACATCAG GGCTCGAGTA GGACCTAAAG GGGAAGCCAG TGAGTTATAT
    GTCACTGAGC CAGAGATGGA TGAAGAACCC AATCAGGAGC CTGGGCAGGA ATTTGTCCCA
    TGTAATTCAG AACTATCTTC TGAAGATGTG GTTCCACTGC CTCCAATCAG GGATCAAGCG
    AACACAAGTG ACTCATCTCA GTTGGCCTCT CACAAATCTT CCTCTGAACC TTCTGAAATA
    AATGGTGGGA ATGATACAGG TTTCAAAGCA TCAGAGAAAG GAGCTGCCCC AGATGCAGCA
    CCAACTCTGC AACTTGTGGA CAGAACAATC CCAACAGTTG AAAACGTGGG AACCAGCATT
    TTTGTAACTC CTCAAGAGCC AACTACGGAC AGCAACAGCT CTCTGATGTC GGAGGACTTT
    TCTCCTAAAA GCCAGACAGA TGCAGGAGCC TTGTTGGACC ATGTTACTAC TGAGAATCCT
    GCCACTGGCC CTGATGCCTT GCTAGCCTCA GATACTTGTC TGGATGTAAG CGAAGCTGGC
    TTTGACCACA GTCTCAGCGA TGCCTCAGGT CTTAACACAT CCACAGGAAC AATAGATGAC
    ATGAGTAAAT TGACATTATC AGAGGGCAAC CCTGAAACAC CAGTTGATGG GGATGCAGGG
    AAACAAGATA TCTGTTCATC TGAAGCCTCG TGGGGTGATT TTGAATATGA TGTCATGGGA
    CAAAATATCG ATGAAGAATT AATGAAAGAG CCTGAACATT TTCTCTACGG GGGTGAGCCT
    CCCCTGGAAG AAGATGCTCT GAAGCAATCG CTGGCACCTT ACACTCCTCC CTTTGATTTG
    TCCTATCTCA CAGAACCTAC TCTTGAGGTC AAAACAACAG AGGGAGCTGG GTCTCCAGAG
    GATGAGTCTT TGGGGAGTGA TGCAGCCGAG ATACTGCTTT CTGCCCTTCC TGATCAGAGA
    AGCAAGGAAA ATCATGCAGA GATCCAAAAC AGCCAGCCTG GGCAGCTGGT TGTGCTGCAT
    ATCCATGAAG ACTCTGAGCC CGTGTCTTTG CCTGCTGGAG TTGAGGCCAA CCTTGAGTTG
    TCTCCATCAA ACATTGACTG GGAAGTAGAA ACAGACAACT CAGATTCACC AGCAGGTGGA
    GACGTAGGAC CACCAAATGG TGTGAGCAAG GGACTGTCAG AGTTGGAAGA AGAAAATGTA
    ATTCCTATCA AAGCGCCTGA GCAGAGAACA GCAGAATGCA GGGAAGAAAG GTACAAAGAG
    AAGAATGAAC ATCATATGGA TTACATACTT GTAAGCCATG GAGAAGATTC AACCCCAATG
    CCAGAGGCCA GTGAAGCCAG ACAAAACACA CCTGAATTAG AAGAGTTTCA CACAGGTTCT
    GAAGACACAG GGCTGCGAGG AACTCAGCTA GCAGGCTTCC CAGGTTCCTG TCAGCCTGCT
    TCCATAAATG AAAGAGAAGA TCATTCTGTA GAGAAAATGT CTCCCAAAGG GGATAAGAGA
    TCATCTCTCG GGAGCCCTGG GCAAGACCCG AGCTGGATGG TCTTAGACCA TATTGAGGTT
    GGTGATCAGC CATTGGAAGC TAGGAACTCG AGGCTTGGAT GCTCTGGCAA GGTCATAGAA
    CTGATCTCTG ATTGTGGCTT GGGCAAGGGT CCCCAAATGC AGGTTCTAGG AGGAGAGAAG
    CCTCTCGAAT CTTTAGCACT AGAAGAGGCC CCTGGTCTGG GCAGCCAGTC ACAGAAGAGC
    AAGAGCCGAG GCAGAGCCAC CCCAGACGCA GTTATGTATC AGGCTGTCAC CCATGACAAT
    GAATGGGAAA TGCTTCCACC ACACCCTTCT CAGAAAAACA AGATCCATGA AACGGAAATG
    GAGGAGGAGA CAGAGTTTCT TGAGCCCAAA ACAAGGAAAC TGAGACCGGA CGGACTGCCA
    TCAGAGGATG TAGGCATGGA CATCTCCTTT GAGGAGGGAA TGCCAAGCCC CAGTGCTGCA
    GACATGAGGC CCGAACCTCC TAATTCTCTA GATCTTAGTG ATGCTCATCC TCGGAGAATC
    AAACTCACAG CCCCAAATAT CAATCTTTCT CTGGACCAAA GTGAAGGATC TGTTCTCTCT
    GATGATAACC TGGACAGTCC AGATGAAATT GATATCAATG TGGATGAACT CGATACCCCT
    GATGAAGCAG ATTCTTTTGA GTACACTGGG CATGAAGTCT CCACATCTAA CAAAGATTCT
    GGCCAAGAGT CAGAGTCTAT TCCGGAGTAT ACAGCAGAAG AGGAGCGAGA GGACAACCGG
    TTGTGGAGAA CTGTGGTGAT TGGAGAACAG GAACAGCGTA TTGACATGAA GGTCATCGAG
    CCCTACAGGA GAGTCATTTC GCATGGAGGA GATTCAGGCT ACTATGGGGA CGGCCTAAAT
    GCCATCATTG TGTTTGCTGC CTGTTTTCTG CCAGACAGCA GTCGGGCGGA TTACCACTAT
    GTCATGGAAA ATCTTTTCCT ATATGTAATA AGTACTTTAG AGCTGATGGT AGCTGAGGAC
    TATATGATTG TGTACTTGAA CGGAGCAACC CCGAGACGGA AGATGCCAGG GCTGGGCTGG
    ATGAAGAAAT GCTATCAGAT GATTGACAGA CGGTTGCGAA AGAATTTGAA ATCATTCATC
    ATTGTTCATC CATCTTGGTT CATCAGGACA ATCCTTGCAG TGACACGACC TTTTATAAGT
    TCAAAATTCA GCAGTAAAAT TAAATATGTC AATAGCTTAT CAGAACTCAG TGGGCTGATC
    CCAATGGATT GTATCCATAT TCCAGAGAGC ATCATCAAGT ACGATGAAGA GAGATCTTAT
    AAGAGAAGTG TGAGCATTGA CCTGAAGCTA AAAGGAAAGC CCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.