RTTN cDNA ORF clone, Canis lupus familiaris(dog)

The following RTTN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RTTN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285803 XM_022413025.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe285804 XM_022413050.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe285805 XM_022413069.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe285799 XM_022412840.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe285800 XM_022412899.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe285801 XM_022412914.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe285802 XM_022412950.1
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe00713 XM_005615328.3
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe00715 XM_005615329.2
Latest version!
Canis lupus familiaris rotatin (RTTN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OCe00713 XM_005615328.2 Canis lupus familiaris rotatin (RTTN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285803
    Clone ID Related Accession (Same CDS sequence) XM_022413025.1
    Accession Version XM_022413025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTAGT
    GATTTAAATG GTTTAGATGA CTCATTCAAG TTTTGGAGAG CTCCATCTAG GATGTCCAAT
    CAAGATCAAG ATCCAAGTTC TCTCTCCACC TTGGAAACAA TGGTGACACC TTCATCGGGG
    AGTACTGAAT TCCCACCCTT TGGGCCCTCA TCGGTGTCTC TCCCTGAGGC TTCACATGAC
    CAGTTTGCGG CTCAAGTAAT AGGTCCACAT GAAACTGCAT CAGCACAGCT TCCTCGTGAC
    ACATCCCTTC CTGCCCCTCT GCCCAAGCAG TGTGTGGTCA CCCCACCTCT CCTTTCAGCA
    GTGTGCAGCC TTCTGGACGG CCTCTTGGTA ATTGCTCCAG GAGACACAGC ACGTGCTTTT
    CGGCAGGCCC GTCTCATAGA GCTTCTCTGT AGTATTATGG ATGCCACCCT CCTAGAGAGC
    TGTGTGCAGG AGCTGAAGGG TCCACTGCCT TTGTCATCTC CAGTTGAACA CACTCAGGCT
    CAGGTGTCCT TTCTCCTAGA ATACCTGTCC TCTTTGTCTA GGCTTCTGCA GTCATGTTTA
    CTGGTAGATC CTGACCTTGT GATTGAGGAT GAGCTTCTGA AACCTCTGGT CACAAACATC
    ATTGGGGTCC TCACCATATG CACCAAAGAT GTGTTAGATG TAGAATTGAT ATCTGCTTTT
    TATCATACAT GGACACATTT ATTTAACCTT TTGGCTACGC TCCTGAGGAA AGCTGCTACT
    GCCTCTCTCT CTTCTATTAT GATGGGTCTG GCCAAGCACT GGGCAACAGT GATTGATATG
    TTGTGCACAT GTGTGAGTTT GTCTACCACA TGCCCTACCT TATCTACTGC CAGCCTGCAG
    TTCCTTTCTA TTCTCTTGAC TGAAGAAGCA AAAAGGCATC TCCAGGATAA GAATAAAACA
    AATTCATGCC ACACTCCATC AATGGCTTCA CTTCTCGATA AAACTCAAGA AAATCAGAAA
    TCTCTAGAAC GACTTAATGA AGTAATTCTT CAGTGCTATG AAGGAAAATC CTCCAAAGAT
    GTCCTGAAAA GAGTTGCTGC AAATACACTG ATGTCACTTC TGGCTGTCAG TAGAAGAGCA
    CAGAGACAGG CTTTGAAAGA TTTTACTTAT TTATTTGAGA GACAAAGAGG GAGCGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268733.1
    CDS287..5986
    Translation

    Target ORF information:

    RefSeq Version XM_022413025.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTAGT
    GATTTAAATG GTTTAGATGA CTCATTCAAG TTTTGGAGAG CTCCATCTAG GATGTCCAAT
    CAAGATCAAG ATCCAAGTTC TCTCTCCACC TTGGAAACAA TGGTGACACC TTCATCGGGG
    AGTACTGAAT TCCCACCCTT TGGGCCCTCA TCGGTGTCTC TCCCTGAGGC TTCACATGAC
    CAGTTTGCGG CTCAAGTAAT AGGTCCACAT GAAACTGCAT CAGCACAGCT TCCTCGTGAC
    ACATCCCTTC CTGCCCCTCT GCCCAAGCAG TGTGTGGTCA CCCCACCTCT CCTTTCAGCA
    GTGTGCAGCC TTCTGGACGG CCTCTTGGTA ATTGCTCCAG GAGACACAGC ACGTGCTTTT
    CGGCAGGCCC GTCTCATAGA GCTTCTCTGT AGTATTATGG ATGCCACCCT CCTAGAGAGC
    TGTGTGCAGG AGCTGAAGGG TCCACTGCCT TTGTCATCTC CAGTTGAACA CACTCAGGCT
    CAGGTGTCCT TTCTCCTAGA ATACCTGTCC TCTTTGTCTA GGCTTCTGCA GTCATGTTTA
    CTGGTAGATC CTGACCTTGT GATTGAGGAT GAGCTTCTGA AACCTCTGGT CACAAACATC
    ATTGGGGTCC TCACCATATG CACCAAAGAT GTGTTAGATG TAGAATTGAT ATCTGCTTTT
    TATCATACAT GGACACATTT ATTTAACCTT TTGGCTACGC TCCTGAGGAA AGCTGCTACT
    GCCTCTCTCT CTTCTATTAT GATGGGTCTG GCCAAGCACT GGGCAACAGT GATTGATATG
    TTGTGCACAT GTGTGAGTTT GTCTACCACA TGCCCTACCT TATCTACTGC CAGCCTGCAG
    TTCCTTTCTA TTCTCTTGAC TGAAGAAGCA AAAAGGCATC TCCAGGATAA GAATAAAACA
    AATTCATGCC ACACTCCATC AATGGCTTCA CTTCTCGATA AAACTCAAGA AAATCAGAAA
    TCTCTAGAAC GACTTAATGA AGTAATTCTT CAGTGCTATG AAGGAAAATC CTCCAAAGAT
    GTCCTGAAAA GAGTTGCTGC AAATACACTG ATGTCACTTC TGGCTGTCAG TAGAAGAGCA
    CAGAGACAGG CTTTGAAAGA TTTTACTTAT TTATTTGAGA GACAAAGAGG GAGCGCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285804
    Clone ID Related Accession (Same CDS sequence) XM_022413050.1
    Accession Version XM_022413050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5682bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTAGT
    GATTTAAATG GTTTAGATGA CTCATTCAAG TTTTGGAGAG CTCCATCTAG GATGTCCAAT
    CAAGATCAAG ATCCAAGTTC TCTCTCCACC TTGGAAACAA TGGTGACACC TTCATCGGGG
    AGTACTGAAT TCCCACCCTT TGGGCCCTCA TCGGTGTCTC TCCCTGAGGC TTCACATGAC
    CAGTTTGCGG CTCAAGTAAT AGGTCCACAT GAAACTGCAT CAGCACAGCT TCCTCGTGAC
    ACATCCCTTC CTGCCCCTCT GCCCAAGCAG TGTGTGGTCA CCCCACCTCT CCTTTCAGCA
    GTGTGCAGCC TTCTGGACGG CCTCTTGGTA ATTGCTCCAG GAGACACAGC ACGTGCTTTT
    CGGCAGGCCC GTCTCATAGA GCTTCTCTGT AGTATTATGG ATGCCACCCT CCTAGAGAGC
    TGTGTGCAGG AGCTGAAGGG TCCACTGCCT TTGTCATCTC CAGTTGAACA CACTCAGGCT
    CAGGTGTCCT TTCTCCTAGA ATACCTGTCC TCTTTGTCTA GGCTTCTGCA GTCATGTTTA
    CTGGTAGATC CTGACCTTGT GATTGAGGAT GAGCTTCTGA AACCTCTGGT CACAAACATC
    ATTGGGGTCC TCACCATATG CACCAAAGAT GTGTTAGATG TAGAATTGAT ATCTGCTTTT
    TATCATACAT GGACACATTT ATTTAACCTT TTGGCTACGC TCCTGAGGAA AGCTGCTACT
    GCCTCTCTCT CTTCTATTAT GATGGGTCTG GCCAAGCACT GGGCAACAGT GATTGATATG
    TTGTGCACAT GTGTGAGTTT GTCTACCACA TGCCCTACCT TATCTACTGC CAGCCTGCAG
    TTCCTTTCTA TTCTCTTGAC TGAAGAAGCA AAAAGGCATC TCCAGGATAA GAATAAAACA
    AATTCATGCC ACACTCCATC AATGGCTTCA CTTCTCGATA AAACTCAAGA AAATCAGAAA
    TCTCTAGAAC GACTTAATGA AGTAATTCTT CAGTGCTATG AAGGAAAATC CTCCAAAGAT
    GTCCTGAAAA GAGTTGCTGC AAATACACTG ATGTCACTTC TGGCTGTCAG TAGAAGAGCA
    CAGAGACAGG CTTTGAAAGA CAAAGAGGGA GCGCATGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268758.1
    CDS287..5968
    Translation

    Target ORF information:

    RefSeq Version XM_022413050.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTAGT
    GATTTAAATG GTTTAGATGA CTCATTCAAG TTTTGGAGAG CTCCATCTAG GATGTCCAAT
    CAAGATCAAG ATCCAAGTTC TCTCTCCACC TTGGAAACAA TGGTGACACC TTCATCGGGG
    AGTACTGAAT TCCCACCCTT TGGGCCCTCA TCGGTGTCTC TCCCTGAGGC TTCACATGAC
    CAGTTTGCGG CTCAAGTAAT AGGTCCACAT GAAACTGCAT CAGCACAGCT TCCTCGTGAC
    ACATCCCTTC CTGCCCCTCT GCCCAAGCAG TGTGTGGTCA CCCCACCTCT CCTTTCAGCA
    GTGTGCAGCC TTCTGGACGG CCTCTTGGTA ATTGCTCCAG GAGACACAGC ACGTGCTTTT
    CGGCAGGCCC GTCTCATAGA GCTTCTCTGT AGTATTATGG ATGCCACCCT CCTAGAGAGC
    TGTGTGCAGG AGCTGAAGGG TCCACTGCCT TTGTCATCTC CAGTTGAACA CACTCAGGCT
    CAGGTGTCCT TTCTCCTAGA ATACCTGTCC TCTTTGTCTA GGCTTCTGCA GTCATGTTTA
    CTGGTAGATC CTGACCTTGT GATTGAGGAT GAGCTTCTGA AACCTCTGGT CACAAACATC
    ATTGGGGTCC TCACCATATG CACCAAAGAT GTGTTAGATG TAGAATTGAT ATCTGCTTTT
    TATCATACAT GGACACATTT ATTTAACCTT TTGGCTACGC TCCTGAGGAA AGCTGCTACT
    GCCTCTCTCT CTTCTATTAT GATGGGTCTG GCCAAGCACT GGGCAACAGT GATTGATATG
    TTGTGCACAT GTGTGAGTTT GTCTACCACA TGCCCTACCT TATCTACTGC CAGCCTGCAG
    TTCCTTTCTA TTCTCTTGAC TGAAGAAGCA AAAAGGCATC TCCAGGATAA GAATAAAACA
    AATTCATGCC ACACTCCATC AATGGCTTCA CTTCTCGATA AAACTCAAGA AAATCAGAAA
    TCTCTAGAAC GACTTAATGA AGTAATTCTT CAGTGCTATG AAGGAAAATC CTCCAAAGAT
    GTCCTGAAAA GAGTTGCTGC AAATACACTG ATGTCACTTC TGGCTGTCAG TAGAAGAGCA
    CAGAGACAGG CTTTGAAAGA CAAAGAGGGA GCGCATGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285805
    Clone ID Related Accession (Same CDS sequence) XM_022413069.1
    Accession Version XM_022413069.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGTGTTGA GGTCCCTGGG AGATACCATA TGCTACCATA AAAGTGGAGT GAGCCAGGAC 
    CAGCCAGAGT TAGTGCTGGT GCACCACCGA ATGGCCTTTG TCAGCGTTTC ACTGTTTGCA
    GTCCGAGTCC TGCAGATGCT TCTCCCTGTT GAAAAGGCAA GCCACCTTTT ACCGGAGCCT
    ATGTCAGCAG CATTATTTCT AGTTTCTTTG GACATGCCTG TTTCTTTGGA ATATCCAAAT
    ATTCATGAAG CTGTTGTGGC ATATTTGGAA CAGTTGAATT CTGAAAACTA CAGTATTTAT
    AAACAAACTG CAGAGGCTGT TTATTCAATT GAATGTACCT GTAACTTCCT GTCTGATGTT
    GGGAAGGAGG GCGAGAAGAA TCTCTTGGAA TTAGTAGAGC TGGCAGACCA AGCCTTGAGA
    AGCTTTTCTT ATCATCAGCA TTTCCCCCTA ATAAAGGAAA TGATCTGCAT GTGTTCAAAG
    ATCTGCAAAT CTGCGCAGGC CAGTCCATTA CTTCAAGGAG AAAGTCAGAA GGTGTTTCTG
    CGCATGTTAT CTCACCCATT ACTGCAAGTG AAAATTGAAA CTTATAACTG CTGTCTGGAA
    ATTACTAAGG AATGTTTAGG TGTCTATAAC GTCACTAAAC CTGTGTCTTC ACTTTGTAAC
    GGAATTCATT TTCTACTTCA CCCGAAAGTT CTGTATGAAA TCTCTGCATT TGGCATTCAA
    GAGCCCAAAA ATGAGGTGAA TGCTGCTGCC AAGGCCATTT TGCTGTATCT GCTGCAGGGA
    CGATTGATGA TGACAGCAGT GACCTGGAAC AAGTTTATTG AGTCTCTCTG TCCTGTCATT
    CCTGTGCTGC AGGGCTATGC TGACACAGAA GATCCTTTGG GTAACTCCAT TCTCCTTCTG
    ACGAAAGGGA ATCCTGAGGC GGGCGAAGGG GCTCTCCCCT GTACCACCCG ACTGAAGTCC
    ATGCTGCGGC TCCTGTTTGT CAGGAAGCCA TTGGTCCGTT CACTAGCATT AAAGCTTTTA
    GCATGTCATC TCACAAGGGA AGAAGGGGCT GACACAAAGC GTCCTTTAAT AGATGCCAGA
    ATTCTCTCCA GAGTTACTAA TTTATTTATT GTGAAAAAAC CTATTGAACT CACGTTGGAT
    GACAGAAGAG AACTGATTAT TAGGTTGGAG ACTGTTGAAA AGGTTTATAA AATCTTTACC
    TCAGATGATA TTGATCTTGT TTTGAGAAAG TCAGCCGCAG AACAGTTAGC TGTGATTATG
    CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTACACT TAGTTGACAC GATAATTGAG
    TATCTAAATG AATGTGTAGG TCAGGATGGT GAGCTCATAG AATGTTTGAT TCTGCCAAGC
    CTCACACTCT TCAGGAAAGT TCTTTGTGCG GACCCGGTCA TGCGCTTGGC CCTCTCACAG
    CAGGCTTCTC TTTTGACCCT GTTATTCAGA GTTTCCTTAA TATTTCATGA AAATTGTGCT
    GTCGTGACTG AAGTCGGAGC ATTATTTTGT CTTCTATTAT TTGATGAAGT ATCAAGAATG
    GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTCAC CATCAGTCTT CAGTTTGCCT
    GTTTCAGTTT TTAGAAGGTA TCATCTACCA GTGCGTGTAG TTGGCCACCA TGCTGTGAGT
    CCTTACTCGA TAGTTTTGCC ATTATCCACT GATTGTTTGG CCTTGAAACC AGTCTCAGAC
    ATGCTGAGAA TAGCTTGGAA CTTGTCCTGG TATCATGGGA GTGAGAATCT GTTGAAGCAA
    ATGAATTCCG AATCTAAAAT CCAAGAATTT TCAGATACAT TGCAGTTAAC CACAGAGGAC
    ACCCTCACTC TGAAGATGAC ACATACAGCT AGTGGACTGA AGGATTGTCT CCATTCCATT
    GTCGAGGCTG CAGCCCACAG GGAAGTGAGG GCTGCTGTCA CAAGAATGAG TTTTTATCTT
    CTGAATGACA GACTGTCTTT AAAGGGTGGC TCTGGCCCAT TTGGGGTTAC CTTGAAGTCC
    TTGTCGTGGC ATACCACACT AAACAGGTTT TTACAGGTAC TTCCTGCTTG CACTGAAGAT
    GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAGT TACTGAAGGA ACAAAGAAAA
    AACTCATCAG TGGAACTTCT AAACTGGCTT CTAGAATTAC TTCTTAGACA TAGTCCAAAC
    CCACTGTTAG ACCTGCTGGT TCTGACGGAG TCACAGGCAC GAGAGGAAAC AGATGACATC
    CAGACTGCTG TCAGGCAACA GCTCCAGAAA GAACTGATTG CTCTTTTCAA TACCTTGCTG
    CTTAGTTTCA TGGCAGTTAC TGACAGAAAA TGCTTGGAAC TTTTTTATGT TTTCCAAACA
    CAGCTGTCTC TGAAACTGCT CCAGTGCCTG AGAGTCACAG ATGCTCCTCA CTTCTATGGC
    CTGCCATCCC TTGAGAGGAC CTTGCGAGGG ATGGCTCATC TCACAGCATC TCCAGGATGG
    AGCTCACATT CTCCTCTCAC AAAGCCATTT GAAATTTGTG TTAAGTACTT GTCAGGTCTA
    CTTGAAGTCA TTACTTCTTT TTATGTGGAG CGAGGAGGAA ATGCTATGTC CTTTATGGGA
    AAAGGGGTCA CTAAGAACAC AGTTCTTTGC TTGCTTCATT TATCCCACGA GATGATGGCC
    CAGGCCCAAA GTTCGGAGTG GATGTCACTT TGGTTTTTGC CTTTGGGCAG TCACAGTGAA
    GGACATGCTA CCCCACAAGG ATTGGCGTGG CTCATTCCAT TATGGGTTGA CCGAGACCCA
    GAGGTGAGGT TCACTTCATT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACTGGCTGT
    GTCGCGTTGG CAAATAGTTG TCAAAACATT TCTGGTGGGC TCTGGGGGAC AGTGGTGAAC
    ATTCTGCTGG ACCAGTCGGA ATGTAGTATG GTGCGCCGGG AGGCTGCATT TATTCTTCAG
    AATCTTCTTG TAATTCCAAT GCCTTCAGTA ATTATAAAGG ATTATACTTG GCAGGGTCCC
    TGTGTCCACG ATGAGGATTC TGGCCTGTCG CTCATAGGAA AACCTGCCCT TCAGGCTCTT
    CTATATCATT GCCATTTCTA TGAACATTTG AATCAGATGA TAAAACACTG TTACCTGGGA
    CGATATACAT TTGATTTGAA TTTTTCTGCT CTGGATGGAA CTTCAGAAGG TAGTGATTTA
    AATGGTTTAG ATGACTCATT CAAGTTTTGG AGAGCTCCAT CTAGGATGTC CAATCAAGAT
    CAAGATCCAA GTTCTCTCTC CACCTTGGAA ACAATGGTGA CACCTTCATC GGGGAGTACT
    GAATTCCCAC CCTTTGGGCC CTCATCGGTG TCTCTCCCTG AGGCTTCACA TGACCAGTTT
    GCGGCTCAAG TAATAGGTCC ACATGAAACT GCATCAGCAC AGCTTCCTCG TGACACATCC
    CTTCCTGCCC CTCTGCCCAA GCAGTGTGTG GTCACCCCAC CTCTCCTTTC AGCAGTGTGC
    AGCCTTCTGG ACGGCCTCTT GGTAATTGCT CCAGGAGACA CAGCACGTGC TTTTCGGCAG
    GCCCGTCTCA TAGAGCTTCT CTGTAGTATT ATGGATGCCA CCCTCCTAGA GAGCTGTGTG
    CAGGAGCTGA AGGGTCCACT GCCTTTGTCA TCTCCAGTTG AACACACTCA GGCTCAGGTG
    TCCTTTCTCC TAGAATACCT GTCCTCTTTG TCTAGGCTTC TGCAGTCATG TTTACTGGTA
    GATCCTGACC TTGTGATTGA GGATGAGCTT CTGAAACCTC TGGTCACAAA CATCATTGGG
    GTCCTCACCA TATGCACCAA AGATGTGTTA GATGTAGAAT TGATATCTGC TTTTTATCAT
    ACATGGACAC ATTTATTTAA CCTTTTGGCT ACGCTCCTGA GGAAAGCTGC TACTGCCTCT
    CTCTCTTCTA TTATGATGGG TCTGGCCAAG CACTGGGCAA CAGTGATTGA TATGTTGTGC
    ACATGTGTGA GTTTGTCTAC CACATGCCCT ACCTTATCTA CTGCCAGCCT GCAGTTCCTT
    TCTATTCTCT TGACTGAAGA AGCAAAAAGG CATCTCCAGG ATAAGAATAA AACAAATTCA
    TGCCACACTC CATCAATGGC TTCACTTCTC GATAAAACTC AAGAAAATCA GAAATCTCTA
    GAACGACTTA ATGAAGTAAT TCTTCAGTGC TATGAAGGAA AATCCTCCAA AGATGTCCTG
    AAAAGAGTTG CTGCAAATAC ACTGATGTCA CTTCTGGCTG TCAGTAGAAG AGCACAGAGA
    CAGGCTTTGA AAGCTGATCT AATAGACAGC TGCATGGAGC AGATGAAGCA CATTAATGCC
    CAGCTGAACC TAGATTCTCT TAGGCCTGGG AAAGCAACCC TAAAGAAACA GGAGGATGGT
    TTTATTAAAG AGTTAAGCAT TGCCATGCAG CTTCTAAGAA ACTGTCTTTA TCAAAATGAA
    GAGTGTAAAG AAGCGGCTCT TGAATCTCAT CTTGTTCCTG TCTTGCACTC TCTCTGGCCT
    TGGCTTTTGA TGGATGATTC ATTGATGCAA ATTGCTCTGC AGCTACTTTG TGTCTATACT
    GCAAATTTTC CAAATGGTTG CAGTTCTCTT TGTTGGTCAA GTTACGGACA ATATCCAGCT
    CAAGCTGCGT ATAGAGGAGC CCCCAGCACC TCTCTGATGG GGTGTATCCT GAAGTTGGCT
    TCCCAGATGC CTCTTGAGAA CACTGCAGTT CAGCAGATGG TTTTTATGTT TCTTTCAAAC
    CTGGCCTTAT CTCATGACTG TAAAGGAGTA ATTCAGAAAA GTAACTTCTT ACAGAACTTT
    CTATCTCTAA CATTGCCAAA AGGAGGAAAT AAACATCTCA GTAATCTGAC CATTCTTTGG
    TTGAAGTTAC TCCTGAATAT ATCATTTGGA GAAGACGGGC AACAAATGAT CCTGAGGCTG
    GATGGCTGTC TAGACTTACT TACAGAGATG AGCAAATATA AGCATAAGAG CAGCCCTTAT
    ATACCTCTTC TTATCTTTCA TAATATTTGC TTCAGTCCTG CCAATAAGCC CAAGATCCTG
    GCTAATGAAA AAGTCATTAG TGTGCTAGCT GCCTGTCTGG AAAGCGAGAA TCAAAACGTT
    CAGAGGATTG GAGCAGCTGC ACTTTGGGCT CTGATTCACA ATTATCAAAA GGCAAAAATG
    ACTTTGAAAA ACCCATCCAT AAAAAGAAGA GTGGATGAAG CATATTCCTT AGCAAAGAAA
    ACTTTCTCAA ACTCAGAAGA AACCCCTCTA AATGCCTATT ACTTGAAATG TCTTGAAAAT
    CTCGTTCAGC TTCTTAACTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268777.1
    CDS122..5488
    Translation

    Target ORF information:

    RefSeq Version XM_022413069.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022413069.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGTGTTGA GGTCCCTGGG AGATACCATA TGCTACCATA AAAGTGGAGT GAGCCAGGAC 
    CAGCCAGAGT TAGTGCTGGT GCACCACCGA ATGGCCTTTG TCAGCGTTTC ACTGTTTGCA
    GTCCGAGTCC TGCAGATGCT TCTCCCTGTT GAAAAGGCAA GCCACCTTTT ACCGGAGCCT
    ATGTCAGCAG CATTATTTCT AGTTTCTTTG GACATGCCTG TTTCTTTGGA ATATCCAAAT
    ATTCATGAAG CTGTTGTGGC ATATTTGGAA CAGTTGAATT CTGAAAACTA CAGTATTTAT
    AAACAAACTG CAGAGGCTGT TTATTCAATT GAATGTACCT GTAACTTCCT GTCTGATGTT
    GGGAAGGAGG GCGAGAAGAA TCTCTTGGAA TTAGTAGAGC TGGCAGACCA AGCCTTGAGA
    AGCTTTTCTT ATCATCAGCA TTTCCCCCTA ATAAAGGAAA TGATCTGCAT GTGTTCAAAG
    ATCTGCAAAT CTGCGCAGGC CAGTCCATTA CTTCAAGGAG AAAGTCAGAA GGTGTTTCTG
    CGCATGTTAT CTCACCCATT ACTGCAAGTG AAAATTGAAA CTTATAACTG CTGTCTGGAA
    ATTACTAAGG AATGTTTAGG TGTCTATAAC GTCACTAAAC CTGTGTCTTC ACTTTGTAAC
    GGAATTCATT TTCTACTTCA CCCGAAAGTT CTGTATGAAA TCTCTGCATT TGGCATTCAA
    GAGCCCAAAA ATGAGGTGAA TGCTGCTGCC AAGGCCATTT TGCTGTATCT GCTGCAGGGA
    CGATTGATGA TGACAGCAGT GACCTGGAAC AAGTTTATTG AGTCTCTCTG TCCTGTCATT
    CCTGTGCTGC AGGGCTATGC TGACACAGAA GATCCTTTGG GTAACTCCAT TCTCCTTCTG
    ACGAAAGGGA ATCCTGAGGC GGGCGAAGGG GCTCTCCCCT GTACCACCCG ACTGAAGTCC
    ATGCTGCGGC TCCTGTTTGT CAGGAAGCCA TTGGTCCGTT CACTAGCATT AAAGCTTTTA
    GCATGTCATC TCACAAGGGA AGAAGGGGCT GACACAAAGC GTCCTTTAAT AGATGCCAGA
    ATTCTCTCCA GAGTTACTAA TTTATTTATT GTGAAAAAAC CTATTGAACT CACGTTGGAT
    GACAGAAGAG AACTGATTAT TAGGTTGGAG ACTGTTGAAA AGGTTTATAA AATCTTTACC
    TCAGATGATA TTGATCTTGT TTTGAGAAAG TCAGCCGCAG AACAGTTAGC TGTGATTATG
    CAAGATATTA AAATGCATGC TGTGGTGAAA AAGTTACACT TAGTTGACAC GATAATTGAG
    TATCTAAATG AATGTGTAGG TCAGGATGGT GAGCTCATAG AATGTTTGAT TCTGCCAAGC
    CTCACACTCT TCAGGAAAGT TCTTTGTGCG GACCCGGTCA TGCGCTTGGC CCTCTCACAG
    CAGGCTTCTC TTTTGACCCT GTTATTCAGA GTTTCCTTAA TATTTCATGA AAATTGTGCT
    GTCGTGACTG AAGTCGGAGC ATTATTTTGT CTTCTATTAT TTGATGAAGT ATCAAGAATG
    GATATGTGGT CTGTTAATCC TTCCAATAAA CCTTCTTCAC CATCAGTCTT CAGTTTGCCT
    GTTTCAGTTT TTAGAAGGTA TCATCTACCA GTGCGTGTAG TTGGCCACCA TGCTGTGAGT
    CCTTACTCGA TAGTTTTGCC ATTATCCACT GATTGTTTGG CCTTGAAACC AGTCTCAGAC
    ATGCTGAGAA TAGCTTGGAA CTTGTCCTGG TATCATGGGA GTGAGAATCT GTTGAAGCAA
    ATGAATTCCG AATCTAAAAT CCAAGAATTT TCAGATACAT TGCAGTTAAC CACAGAGGAC
    ACCCTCACTC TGAAGATGAC ACATACAGCT AGTGGACTGA AGGATTGTCT CCATTCCATT
    GTCGAGGCTG CAGCCCACAG GGAAGTGAGG GCTGCTGTCA CAAGAATGAG TTTTTATCTT
    CTGAATGACA GACTGTCTTT AAAGGGTGGC TCTGGCCCAT TTGGGGTTAC CTTGAAGTCC
    TTGTCGTGGC ATACCACACT AAACAGGTTT TTACAGGTAC TTCCTGCTTG CACTGAAGAT
    GAGAAACTGC TAATAGATAT CATACATTTT TTAAATAAGT TACTGAAGGA ACAAAGAAAA
    AACTCATCAG TGGAACTTCT AAACTGGCTT CTAGAATTAC TTCTTAGACA TAGTCCAAAC
    CCACTGTTAG ACCTGCTGGT TCTGACGGAG TCACAGGCAC GAGAGGAAAC AGATGACATC
    CAGACTGCTG TCAGGCAACA GCTCCAGAAA GAACTGATTG CTCTTTTCAA TACCTTGCTG
    CTTAGTTTCA TGGCAGTTAC TGACAGAAAA TGCTTGGAAC TTTTTTATGT TTTCCAAACA
    CAGCTGTCTC TGAAACTGCT CCAGTGCCTG AGAGTCACAG ATGCTCCTCA CTTCTATGGC
    CTGCCATCCC TTGAGAGGAC CTTGCGAGGG ATGGCTCATC TCACAGCATC TCCAGGATGG
    AGCTCACATT CTCCTCTCAC AAAGCCATTT GAAATTTGTG TTAAGTACTT GTCAGGTCTA
    CTTGAAGTCA TTACTTCTTT TTATGTGGAG CGAGGAGGAA ATGCTATGTC CTTTATGGGA
    AAAGGGGTCA CTAAGAACAC AGTTCTTTGC TTGCTTCATT TATCCCACGA GATGATGGCC
    CAGGCCCAAA GTTCGGAGTG GATGTCACTT TGGTTTTTGC CTTTGGGCAG TCACAGTGAA
    GGACATGCTA CCCCACAAGG ATTGGCGTGG CTCATTCCAT TATGGGTTGA CCGAGACCCA
    GAGGTGAGGT TCACTTCATT GGGATTAGGA TCAGCACTGA CCACCCTTGA AACTGGCTGT
    GTCGCGTTGG CAAATAGTTG TCAAAACATT TCTGGTGGGC TCTGGGGGAC AGTGGTGAAC
    ATTCTGCTGG ACCAGTCGGA ATGTAGTATG GTGCGCCGGG AGGCTGCATT TATTCTTCAG
    AATCTTCTTG TAATTCCAAT GCCTTCAGTA ATTATAAAGG ATTATACTTG GCAGGGTCCC
    TGTGTCCACG ATGAGGATTC TGGCCTGTCG CTCATAGGAA AACCTGCCCT TCAGGCTCTT
    CTATATCATT GCCATTTCTA TGAACATTTG AATCAGATGA TAAAACACTG TTACCTGGGA
    CGATATACAT TTGATTTGAA TTTTTCTGCT CTGGATGGAA CTTCAGAAGG TAGTGATTTA
    AATGGTTTAG ATGACTCATT CAAGTTTTGG AGAGCTCCAT CTAGGATGTC CAATCAAGAT
    CAAGATCCAA GTTCTCTCTC CACCTTGGAA ACAATGGTGA CACCTTCATC GGGGAGTACT
    GAATTCCCAC CCTTTGGGCC CTCATCGGTG TCTCTCCCTG AGGCTTCACA TGACCAGTTT
    GCGGCTCAAG TAATAGGTCC ACATGAAACT GCATCAGCAC AGCTTCCTCG TGACACATCC
    CTTCCTGCCC CTCTGCCCAA GCAGTGTGTG GTCACCCCAC CTCTCCTTTC AGCAGTGTGC
    AGCCTTCTGG ACGGCCTCTT GGTAATTGCT CCAGGAGACA CAGCACGTGC TTTTCGGCAG
    GCCCGTCTCA TAGAGCTTCT CTGTAGTATT ATGGATGCCA CCCTCCTAGA GAGCTGTGTG
    CAGGAGCTGA AGGGTCCACT GCCTTTGTCA TCTCCAGTTG AACACACTCA GGCTCAGGTG
    TCCTTTCTCC TAGAATACCT GTCCTCTTTG TCTAGGCTTC TGCAGTCATG TTTACTGGTA
    GATCCTGACC TTGTGATTGA GGATGAGCTT CTGAAACCTC TGGTCACAAA CATCATTGGG
    GTCCTCACCA TATGCACCAA AGATGTGTTA GATGTAGAAT TGATATCTGC TTTTTATCAT
    ACATGGACAC ATTTATTTAA CCTTTTGGCT ACGCTCCTGA GGAAAGCTGC TACTGCCTCT
    CTCTCTTCTA TTATGATGGG TCTGGCCAAG CACTGGGCAA CAGTGATTGA TATGTTGTGC
    ACATGTGTGA GTTTGTCTAC CACATGCCCT ACCTTATCTA CTGCCAGCCT GCAGTTCCTT
    TCTATTCTCT TGACTGAAGA AGCAAAAAGG CATCTCCAGG ATAAGAATAA AACAAATTCA
    TGCCACACTC CATCAATGGC TTCACTTCTC GATAAAACTC AAGAAAATCA GAAATCTCTA
    GAACGACTTA ATGAAGTAAT TCTTCAGTGC TATGAAGGAA AATCCTCCAA AGATGTCCTG
    AAAAGAGTTG CTGCAAATAC ACTGATGTCA CTTCTGGCTG TCAGTAGAAG AGCACAGAGA
    CAGGCTTTGA AAGCTGATCT AATAGACAGC TGCATGGAGC AGATGAAGCA CATTAATGCC
    CAGCTGAACC TAGATTCTCT TAGGCCTGGG AAAGCAACCC TAAAGAAACA GGAGGATGGT
    TTTATTAAAG AGTTAAGCAT TGCCATGCAG CTTCTAAGAA ACTGTCTTTA TCAAAATGAA
    GAGTGTAAAG AAGCGGCTCT TGAATCTCAT CTTGTTCCTG TCTTGCACTC TCTCTGGCCT
    TGGCTTTTGA TGGATGATTC ATTGATGCAA ATTGCTCTGC AGCTACTTTG TGTCTATACT
    GCAAATTTTC CAAATGGTTG CAGTTCTCTT TGTTGGTCAA GTTACGGACA ATATCCAGCT
    CAAGCTGCGT ATAGAGGAGC CCCCAGCACC TCTCTGATGG GGTGTATCCT GAAGTTGGCT
    TCCCAGATGC CTCTTGAGAA CACTGCAGTT CAGCAGATGG TTTTTATGTT TCTTTCAAAC
    CTGGCCTTAT CTCATGACTG TAAAGGAGTA ATTCAGAAAA GTAACTTCTT ACAGAACTTT
    CTATCTCTAA CATTGCCAAA AGGAGGAAAT AAACATCTCA GTAATCTGAC CATTCTTTGG
    TTGAAGTTAC TCCTGAATAT ATCATTTGGA GAAGACGGGC AACAAATGAT CCTGAGGCTG
    GATGGCTGTC TAGACTTACT TACAGAGATG AGCAAATATA AGCATAAGAG CAGCCCTTAT
    ATACCTCTTC TTATCTTTCA TAATATTTGC TTCAGTCCTG CCAATAAGCC CAAGATCCTG
    GCTAATGAAA AAGTCATTAG TGTGCTAGCT GCCTGTCTGG AAAGCGAGAA TCAAAACGTT
    CAGAGGATTG GAGCAGCTGC ACTTTGGGCT CTGATTCACA ATTATCAAAA GGCAAAAATG
    ACTTTGAAAA ACCCATCCAT AAAAAGAAGA GTGGATGAAG CATATTCCTT AGCAAAGAAA
    ACTTTCTCAA ACTCAGAAGA AACCCCTCTA AATGCCTATT ACTTGAAATG TCTTGAAAAT
    CTCGTTCAGC TTCTTAACTC TTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285799
    Clone ID Related Accession (Same CDS sequence) XM_022412840.1
    Accession Version XM_022412840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTAGT
    GATTTAAATG GTTTAGATGA CTCATTCAAG TTTTGGAGAG CTCCATCTAG GATGTCCAAT
    CAAGATCAAG ATCCAAGTTC TCTCTCCACC TTGGAAACAA TGGTGACACC TTCATCGGGG
    AGTACTGAAT TCCCACCCTT TGGGCCCTCA TCGGTGTCTC TCCCTGAGGC TTCACATGAC
    CAGTTTGCGG CTCAAGTAAT AGGTCCACAT GAAACTGCAT CAGCACAGCT TCCTCGTGAC
    ACATCCCTTC CTGCCCCTCT GCCCAAGCAG TGTGTGGTCA CCCCACCTCT CCTTTCAGCA
    GTGTGCAGCC TTCTGGACGG CCTCTTGGTA ATTGCTCCAG GAGACACAGC ACGTGCTTTT
    CGGCAGGCCC GTCTCATAGA GCTTCTCTGT AGTATTATGG ATGCCACCCT CCTAGAGAGC
    TGTGTGCAGG AGCTGAAGGG TCCACTGCCT TTGTCATCTC CAGTTGAACA CACTCAGGCT
    CAGGTGTCCT TTCTCCTAGA ATACCTGTCC TCTTTGTCTA GGCTTCTGCA GTCATGTTTA
    CTGGTAGATC CTGACCTTGT GATTGAGGAT GAGCTTCTGA AACCTCTGGT CACAAACATC
    ATTGGGGTCC TCACCATATG CACCAAAGAT GTGTTAGATG TAGAATTGAT ATCTGCTTTT
    TATCATACAT GGACACATTT ATTTAACCTT TTGGCTACGC TCCTGAGGAA AGCTGCTACT
    GCCTCTCTCT CTTCTATTAT GATGGGTCTG GCCAAGCACT GGGCAACAGT GATTGATATG
    TTGTGCACAT GTGTGAGTTT GTCTACCACA TGCCCTACCT TATCTACTGC CAGCCTGCAG
    TTCCTTTCTA TTCTCTTGAC TGAAGAAGCA AAAAGGCATC TCCAGGATAA GAATAAAACA
    AATTCATGCC ACACTCCATC AATGGCTTCA CTTCTCGATA AAACTCAAGA AAATCAGAAA
    TCTCTAGAAC GACTTAATGA AGTAATTCTT CAGTGCTATG AAGGAAAATC CTCCAAAGAT
    GTCCTGAAAA GAGTTGCTGC AAATACACTG ATGTCACTTC TGGCTGTCAG TAGAAGAGCA
    CAGAGACAGG CTTTGAAAGC TGATCTAATA GACAGCTGCA TGGAGCAGAT GAAGCACATT
    AATGCCCAGC TGAACCTAGA TTCTCTTAGG CCTGGGAAAG CAACCCTAAA GAAACAGGAG
    GATGGTTTTA TTAAAGAGTT AAGCATTGCC ATGCAGCTTC TAAGAAACTG TCTTTATCAA
    AATGAAGAGT GTAAAGAAGC GGCTCTTGAA TCTCATCTTG TTCCTGTCTT GCACTCTCTC
    TGGCCTTGGC TTTTGATGGA TGATTCATTG ATGCAAATTG CTCTGCAGCT ACTTTGTGTC
    TATACTGCAA ATTTTCCAAA TGGTTGCAGT TCTCTTTGTT GGTCAAGTTA CGGACAATAT
    CCAGCTCAAG CTGCGTATAG AGGAGCCCCC AGCACCTCTC TGATGGGGTG TATCCTGAAG
    TTGGCTTCCC AGATGCCTCT TGAGAACACT GCAGTTCAGC AGATGGTTTT TATGTTTCTT
    TCAAACCTGG CCTTATCTCA TGACTGTAAA GGAGTAATTC AGAAAAGTAA CTTCTTACAG
    AACTTTCTAT CTCTAACATT GCCAAAAGGA GGAAATAAAC ATCTCAGTAA TCTGACCATT
    CTTTGGTTGA AGTTACTCCT GAATATATCA TTTGGAGAAG ACGGGCAACA AATGATCCTG
    AGGCTGGATG GCTGTCTAGA CTTACTTACA GAGATGAGCA AATATAAGCA TAAGAGCAGC
    CCTTATATAC CTCTTCTTAT CTTTCATAAT ATTTGCTTCA GTCCTGCCAA TAAGCCCAAG
    ATCCTGGCTA ATGAAAAAGT CATTAGTGTG CTAGCTGCCT GTCTGGAAAG CGAGAATCAA
    AACGTTCAGA GGATTGGAGC AGCTGCACTT TGGGCTCTGA TTCACAATTA TCAAAAGGCA
    AAAATGACTT TGAAAAACCC ATCCATAAAA AGAAGAGTGG ATGAAGCATA TTCCTTAGCA
    AAGAAAACTT TCTCAAACTC AGAAGAAACC CCTCTAAATG CCTATTACTT GAAATGTCTT
    GAAAATCTCG TTCAGCTTCT TAACTCTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268548.1
    CDS281..6973
    Translation

    Target ORF information:

    RefSeq Version XM_022412840.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTAGT
    GATTTAAATG GTTTAGATGA CTCATTCAAG TTTTGGAGAG CTCCATCTAG GATGTCCAAT
    CAAGATCAAG ATCCAAGTTC TCTCTCCACC TTGGAAACAA TGGTGACACC TTCATCGGGG
    AGTACTGAAT TCCCACCCTT TGGGCCCTCA TCGGTGTCTC TCCCTGAGGC TTCACATGAC
    CAGTTTGCGG CTCAAGTAAT AGGTCCACAT GAAACTGCAT CAGCACAGCT TCCTCGTGAC
    ACATCCCTTC CTGCCCCTCT GCCCAAGCAG TGTGTGGTCA CCCCACCTCT CCTTTCAGCA
    GTGTGCAGCC TTCTGGACGG CCTCTTGGTA ATTGCTCCAG GAGACACAGC ACGTGCTTTT
    CGGCAGGCCC GTCTCATAGA GCTTCTCTGT AGTATTATGG ATGCCACCCT CCTAGAGAGC
    TGTGTGCAGG AGCTGAAGGG TCCACTGCCT TTGTCATCTC CAGTTGAACA CACTCAGGCT
    CAGGTGTCCT TTCTCCTAGA ATACCTGTCC TCTTTGTCTA GGCTTCTGCA GTCATGTTTA
    CTGGTAGATC CTGACCTTGT GATTGAGGAT GAGCTTCTGA AACCTCTGGT CACAAACATC
    ATTGGGGTCC TCACCATATG CACCAAAGAT GTGTTAGATG TAGAATTGAT ATCTGCTTTT
    TATCATACAT GGACACATTT ATTTAACCTT TTGGCTACGC TCCTGAGGAA AGCTGCTACT
    GCCTCTCTCT CTTCTATTAT GATGGGTCTG GCCAAGCACT GGGCAACAGT GATTGATATG
    TTGTGCACAT GTGTGAGTTT GTCTACCACA TGCCCTACCT TATCTACTGC CAGCCTGCAG
    TTCCTTTCTA TTCTCTTGAC TGAAGAAGCA AAAAGGCATC TCCAGGATAA GAATAAAACA
    AATTCATGCC ACACTCCATC AATGGCTTCA CTTCTCGATA AAACTCAAGA AAATCAGAAA
    TCTCTAGAAC GACTTAATGA AGTAATTCTT CAGTGCTATG AAGGAAAATC CTCCAAAGAT
    GTCCTGAAAA GAGTTGCTGC AAATACACTG ATGTCACTTC TGGCTGTCAG TAGAAGAGCA
    CAGAGACAGG CTTTGAAAGC TGATCTAATA GACAGCTGCA TGGAGCAGAT GAAGCACATT
    AATGCCCAGC TGAACCTAGA TTCTCTTAGG CCTGGGAAAG CAACCCTAAA GAAACAGGAG
    GATGGTTTTA TTAAAGAGTT AAGCATTGCC ATGCAGCTTC TAAGAAACTG TCTTTATCAA
    AATGAAGAGT GTAAAGAAGC GGCTCTTGAA TCTCATCTTG TTCCTGTCTT GCACTCTCTC
    TGGCCTTGGC TTTTGATGGA TGATTCATTG ATGCAAATTG CTCTGCAGCT ACTTTGTGTC
    TATACTGCAA ATTTTCCAAA TGGTTGCAGT TCTCTTTGTT GGTCAAGTTA CGGACAATAT
    CCAGCTCAAG CTGCGTATAG AGGAGCCCCC AGCACCTCTC TGATGGGGTG TATCCTGAAG
    TTGGCTTCCC AGATGCCTCT TGAGAACACT GCAGTTCAGC AGATGGTTTT TATGTTTCTT
    TCAAACCTGG CCTTATCTCA TGACTGTAAA GGAGTAATTC AGAAAAGTAA CTTCTTACAG
    AACTTTCTAT CTCTAACATT GCCAAAAGGA GGAAATAAAC ATCTCAGTAA TCTGACCATT
    CTTTGGTTGA AGTTACTCCT GAATATATCA TTTGGAGAAG ACGGGCAACA AATGATCCTG
    AGGCTGGATG GCTGTCTAGA CTTACTTACA GAGATGAGCA AATATAAGCA TAAGAGCAGC
    CCTTATATAC CTCTTCTTAT CTTTCATAAT ATTTGCTTCA GTCCTGCCAA TAAGCCCAAG
    ATCCTGGCTA ATGAAAAAGT CATTAGTGTG CTAGCTGCCT GTCTGGAAAG CGAGAATCAA
    AACGTTCAGA GGATTGGAGC AGCTGCACTT TGGGCTCTGA TTCACAATTA TCAAAAGGCA
    AAAATGACTT TGAAAAACCC ATCCATAAAA AGAAGAGTGG ATGAAGCATA TTCCTTAGCA
    AAGAAAACTT TCTCAAACTC AGAAGAAACC CCTCTAAATG CCTATTACTT GAAATGTCTT
    GAAAATCTCG TTCAGCTTCT TAACTCTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285800
    Clone ID Related Accession (Same CDS sequence) XM_022412899.1
    Accession Version XM_022412899.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTATCA TCTACCAGTG CGTGTAGTTG GCCACCATGC TGTGAGTCCT
    TACTCGATAG TTTTGCCATT ATCCACTGAT TGTTTGGCCT TGAAACCAGT CTCAGACATG
    CTGAGAATAG CTTGGAACTT GTCCTGGTAT CATGGGAGTG AGAATCTGTT GAAGCAAATG
    AATTCCGAAT CTAAAATCCA AGAATTTTCA GATACATTGC AGTTAACCAC AGAGGACACC
    CTCACTCTGA AGATGACACA TACAGCTAGT GGACTGAAGG ATTGTCTCCA TTCCATTGTC
    GAGGCTGCAG CCCACAGGGA AGTGAGGGCT GCTGTCACAA GAATGAGTTT TTATCTTCTG
    AATGACAGAC TGTCTTTAAA GGGTGGCTCT GGCCCATTTG GGGTTACCTT GAAGTCCTTG
    TCGTGGCATA CCACACTAAA CAGGTTTTTA CAGGTACTTC CTGCTTGCAC TGAAGATGAG
    AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAGTTAC TGAAGGAACA AAGAAAAAAC
    TCATCAGTGG AACTTCTAAA CTGGCTTCTA GAATTACTTC TTAGACATAG TCCAAACCCA
    CTGTTAGACC TGCTGGTTCT GACGGAGTCA CAGGCACGAG AGGAAACAGA TGACATCCAG
    ACTGCTGTCA GGCAACAGCT CCAGAAAGAA CTGATTGCTC TTTTCAATAC CTTGCTGCTT
    AGTTTCATGG CAGTTACTGA CAGAAAATGC TTGGAACTTT TTTATGTTTT CCAAACACAG
    CTGTCTCTGA AACTGCTCCA GTGCCTGAGA GTCACAGATG CTCCTCACTT CTATGGCCTG
    CCATCCCTTG AGAGGACCTT GCGAGGGATG GCTCATCTCA CAGCATCTCC AGGATGGAGC
    TCACATTCTC CTCTCACAAA GCCATTTGAA ATTTGTGTTA AGTACTTGTC AGGTCTACTT
    GAAGTCATTA CTTCTTTTTA TGTGGAGCGA GGAGGAAATG CTATGTCCTT TATGGGAAAA
    GGGGTCACTA AGAACACAGT TCTTTGCTTG CTTCATTTAT CCCACGAGAT GATGGCCCAG
    GCCCAAAGTT CGGAGTGGAT GTCACTTTGG TTTTTGCCTT TGGGCAGTCA CAGTGAAGGA
    CATGCTACCC CACAAGGATT GGCGTGGCTC ATTCCATTAT GGGTTGACCG AGACCCAGAG
    GTGAGGTTCA CTTCATTGGG ATTAGGATCA GCACTGACCA CCCTTGAAAC TGGCTGTGTC
    GCGTTGGCAA ATAGTTGTCA AAACATTTCT GGTGGGCTCT GGGGGACAGT GGTGAACATT
    CTGCTGGACC AGTCGGAATG TAGTATGGTG CGCCGGGAGG CTGCATTTAT TCTTCAGAAT
    CTTCTTGTAA TTCCAATGCC TTCAGTAATT ATAAAGGATT ATACTTGGCA GGGTCCCTGT
    GTCCACGATG AGGATTCTGG CCTGTCGCTC ATAGGAAAAC CTGCCCTTCA GGCTCTTCTA
    TATCATTGCC ATTTCTATGA ACATTTGAAT CAGATGATAA AACACTGTTA CCTGGGACGA
    TATACATTTG ATTTGAATTT TTCTGCTCTG GATGGAACTT CAGAAGGTAG TGATTTAAAT
    GGTTTAGATG ACTCATTCAA GTTTTGGAGA GCTCCATCTA GGATGTCCAA TCAAGATCAA
    GATCCAAGTT CTCTCTCCAC CTTGGAAACA ATGGTGACAC CTTCATCGGG GAGTACTGAA
    TTCCCACCCT TTGGGCCCTC ATCGGTGTCT CTCCCTGAGG CTTCACATGA CCAGTTTGCG
    GCTCAAGTAA TAGGTCCACA TGAAACTGCA TCAGCACAGC TTCCTCGTGA CACATCCCTT
    CCTGCCCCTC TGCCCAAGCA GTGTGTGGTC ACCCCACCTC TCCTTTCAGC AGTGTGCAGC
    CTTCTGGACG GCCTCTTGGT AATTGCTCCA GGAGACACAG CACGTGCTTT TCGGCAGGCC
    CGTCTCATAG AGCTTCTCTG TAGTATTATG GATGCCACCC TCCTAGAGAG CTGTGTGCAG
    GAGCTGAAGG GTCCACTGCC TTTGTCATCT CCAGTTGAAC ACACTCAGGC TCAGGTGTCC
    TTTCTCCTAG AATACCTGTC CTCTTTGTCT AGGCTTCTGC AGTCATGTTT ACTGGTAGAT
    CCTGACCTTG TGATTGAGGA TGAGCTTCTG AAACCTCTGG TCACAAACAT CATTGGGGTC
    CTCACCATAT GCACCAAAGA TGTGTTAGAT GTAGAATTGA TATCTGCTTT TTATCATACA
    TGGACACATT TATTTAACCT TTTGGCTACG CTCCTGAGGA AAGCTGCTAC TGCCTCTCTC
    TCTTCTATTA TGATGGGTCT GGCCAAGCAC TGGGCAACAG TGATTGATAT GTTGTGCACA
    TGTGTGAGTT TGTCTACCAC ATGCCCTACC TTATCTACTG CCAGCCTGCA GTTCCTTTCT
    ATTCTCTTGA CTGAAGAAGC AAAAAGGCAT CTCCAGGATA AGAATAAAAC AAATTCATGC
    CACACTCCAT CAATGGCTTC ACTTCTCGAT AAAACTCAAG AAAATCAGAA ATCTCTAGAA
    CGACTTAATG AAGTAATTCT TCAGTGCTAT GAAGGAAAAT CCTCCAAAGA TGTCCTGAAA
    AGAGTTGCTG CAAATACACT GATGTCACTT CTGGCTGTCA GTAGAAGAGC ACAGAGACAG
    GCTTTGAAAG CTGATCTAAT AGACAGCTGC ATGGAGCAGA TGAAGCACAT TAATGCCCAG
    CTGAACCTAG ATTCTCTTAG GCCTGGGAAA GCAACCCTAA AGAAACAGGA GGATGGTTTT
    ATTAAAGAGT TAAGCATTGC CATGCAGCTT CTAAGAAACT GTCTTTATCA AAATGAAGAG
    TGTAAAGAAG CGGCTCTTGA ATCTCATCTT GTTCCTGTCT TGCACTCTCT CTGGCCTTGG
    CTTTTGATGG ATGATTCATT GATGCAAATT GCTCTGCAGC TACTTTGTGT CTATACTGCA
    AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT ACGGACAATA TCCAGCTCAA
    GCTGCGTATA GAGGAGCCCC CAGCACCTCT CTGATGGGGT GTATCCTGAA GTTGGCTTCC
    CAGATGCCTC TTGAGAACAC TGCAGTTCAG CAGATGGTTT TTATGTTTCT TTCAAACCTG
    GCCTTATCTC ATGACTGTAA AGGAGTAATT CAGAAAAGTA ACTTCTTACA GAACTTTCTA
    TCTCTAACAT TGCCAAAAGG AGGAAATAAA CATCTCAGTA ATCTGACCAT TCTTTGGTTG
    AAGTTACTCC TGAATATATC ATTTGGAGAA GACGGGCAAC AAATGATCCT GAGGCTGGAT
    GGCTGTCTAG ACTTACTTAC AGAGATGAGC AAATATAAGC ATAAGAGCAG CCCTTATATA
    CCTCTTCTTA TCTTTCATAA TATTTGCTTC AGTCCTGCCA ATAAGCCCAA GATCCTGGCT
    AATGAAAAAG TCATTAGTGT GCTAGCTGCC TGTCTGGAAA GCGAGAATCA AAACGTTCAG
    AGGATTGGAG CAGCTGCACT TTGGGCTCTG ATTCACAATT ATCAAAAGGC AAAAATGACT
    TTGAAAAACC CATCCATAAA AAGAAGAGTG GATGAAGCAT ATTCCTTAGC AAAGAAAACT
    TTCTCAAACT CAGAAGAAAC CCCTCTAAAT GCCTATTACT TGAAATGTCT TGAAAATCTC
    GTTCAGCTTC TTAACTCTTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268607.1
    CDS277..6900
    Translation

    Target ORF information:

    RefSeq Version XM_022412899.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412899.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTATCA TCTACCAGTG CGTGTAGTTG GCCACCATGC TGTGAGTCCT
    TACTCGATAG TTTTGCCATT ATCCACTGAT TGTTTGGCCT TGAAACCAGT CTCAGACATG
    CTGAGAATAG CTTGGAACTT GTCCTGGTAT CATGGGAGTG AGAATCTGTT GAAGCAAATG
    AATTCCGAAT CTAAAATCCA AGAATTTTCA GATACATTGC AGTTAACCAC AGAGGACACC
    CTCACTCTGA AGATGACACA TACAGCTAGT GGACTGAAGG ATTGTCTCCA TTCCATTGTC
    GAGGCTGCAG CCCACAGGGA AGTGAGGGCT GCTGTCACAA GAATGAGTTT TTATCTTCTG
    AATGACAGAC TGTCTTTAAA GGGTGGCTCT GGCCCATTTG GGGTTACCTT GAAGTCCTTG
    TCGTGGCATA CCACACTAAA CAGGTTTTTA CAGGTACTTC CTGCTTGCAC TGAAGATGAG
    AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAGTTAC TGAAGGAACA AAGAAAAAAC
    TCATCAGTGG AACTTCTAAA CTGGCTTCTA GAATTACTTC TTAGACATAG TCCAAACCCA
    CTGTTAGACC TGCTGGTTCT GACGGAGTCA CAGGCACGAG AGGAAACAGA TGACATCCAG
    ACTGCTGTCA GGCAACAGCT CCAGAAAGAA CTGATTGCTC TTTTCAATAC CTTGCTGCTT
    AGTTTCATGG CAGTTACTGA CAGAAAATGC TTGGAACTTT TTTATGTTTT CCAAACACAG
    CTGTCTCTGA AACTGCTCCA GTGCCTGAGA GTCACAGATG CTCCTCACTT CTATGGCCTG
    CCATCCCTTG AGAGGACCTT GCGAGGGATG GCTCATCTCA CAGCATCTCC AGGATGGAGC
    TCACATTCTC CTCTCACAAA GCCATTTGAA ATTTGTGTTA AGTACTTGTC AGGTCTACTT
    GAAGTCATTA CTTCTTTTTA TGTGGAGCGA GGAGGAAATG CTATGTCCTT TATGGGAAAA
    GGGGTCACTA AGAACACAGT TCTTTGCTTG CTTCATTTAT CCCACGAGAT GATGGCCCAG
    GCCCAAAGTT CGGAGTGGAT GTCACTTTGG TTTTTGCCTT TGGGCAGTCA CAGTGAAGGA
    CATGCTACCC CACAAGGATT GGCGTGGCTC ATTCCATTAT GGGTTGACCG AGACCCAGAG
    GTGAGGTTCA CTTCATTGGG ATTAGGATCA GCACTGACCA CCCTTGAAAC TGGCTGTGTC
    GCGTTGGCAA ATAGTTGTCA AAACATTTCT GGTGGGCTCT GGGGGACAGT GGTGAACATT
    CTGCTGGACC AGTCGGAATG TAGTATGGTG CGCCGGGAGG CTGCATTTAT TCTTCAGAAT
    CTTCTTGTAA TTCCAATGCC TTCAGTAATT ATAAAGGATT ATACTTGGCA GGGTCCCTGT
    GTCCACGATG AGGATTCTGG CCTGTCGCTC ATAGGAAAAC CTGCCCTTCA GGCTCTTCTA
    TATCATTGCC ATTTCTATGA ACATTTGAAT CAGATGATAA AACACTGTTA CCTGGGACGA
    TATACATTTG ATTTGAATTT TTCTGCTCTG GATGGAACTT CAGAAGGTAG TGATTTAAAT
    GGTTTAGATG ACTCATTCAA GTTTTGGAGA GCTCCATCTA GGATGTCCAA TCAAGATCAA
    GATCCAAGTT CTCTCTCCAC CTTGGAAACA ATGGTGACAC CTTCATCGGG GAGTACTGAA
    TTCCCACCCT TTGGGCCCTC ATCGGTGTCT CTCCCTGAGG CTTCACATGA CCAGTTTGCG
    GCTCAAGTAA TAGGTCCACA TGAAACTGCA TCAGCACAGC TTCCTCGTGA CACATCCCTT
    CCTGCCCCTC TGCCCAAGCA GTGTGTGGTC ACCCCACCTC TCCTTTCAGC AGTGTGCAGC
    CTTCTGGACG GCCTCTTGGT AATTGCTCCA GGAGACACAG CACGTGCTTT TCGGCAGGCC
    CGTCTCATAG AGCTTCTCTG TAGTATTATG GATGCCACCC TCCTAGAGAG CTGTGTGCAG
    GAGCTGAAGG GTCCACTGCC TTTGTCATCT CCAGTTGAAC ACACTCAGGC TCAGGTGTCC
    TTTCTCCTAG AATACCTGTC CTCTTTGTCT AGGCTTCTGC AGTCATGTTT ACTGGTAGAT
    CCTGACCTTG TGATTGAGGA TGAGCTTCTG AAACCTCTGG TCACAAACAT CATTGGGGTC
    CTCACCATAT GCACCAAAGA TGTGTTAGAT GTAGAATTGA TATCTGCTTT TTATCATACA
    TGGACACATT TATTTAACCT TTTGGCTACG CTCCTGAGGA AAGCTGCTAC TGCCTCTCTC
    TCTTCTATTA TGATGGGTCT GGCCAAGCAC TGGGCAACAG TGATTGATAT GTTGTGCACA
    TGTGTGAGTT TGTCTACCAC ATGCCCTACC TTATCTACTG CCAGCCTGCA GTTCCTTTCT
    ATTCTCTTGA CTGAAGAAGC AAAAAGGCAT CTCCAGGATA AGAATAAAAC AAATTCATGC
    CACACTCCAT CAATGGCTTC ACTTCTCGAT AAAACTCAAG AAAATCAGAA ATCTCTAGAA
    CGACTTAATG AAGTAATTCT TCAGTGCTAT GAAGGAAAAT CCTCCAAAGA TGTCCTGAAA
    AGAGTTGCTG CAAATACACT GATGTCACTT CTGGCTGTCA GTAGAAGAGC ACAGAGACAG
    GCTTTGAAAG CTGATCTAAT AGACAGCTGC ATGGAGCAGA TGAAGCACAT TAATGCCCAG
    CTGAACCTAG ATTCTCTTAG GCCTGGGAAA GCAACCCTAA AGAAACAGGA GGATGGTTTT
    ATTAAAGAGT TAAGCATTGC CATGCAGCTT CTAAGAAACT GTCTTTATCA AAATGAAGAG
    TGTAAAGAAG CGGCTCTTGA ATCTCATCTT GTTCCTGTCT TGCACTCTCT CTGGCCTTGG
    CTTTTGATGG ATGATTCATT GATGCAAATT GCTCTGCAGC TACTTTGTGT CTATACTGCA
    AATTTTCCAA ATGGTTGCAG TTCTCTTTGT TGGTCAAGTT ACGGACAATA TCCAGCTCAA
    GCTGCGTATA GAGGAGCCCC CAGCACCTCT CTGATGGGGT GTATCCTGAA GTTGGCTTCC
    CAGATGCCTC TTGAGAACAC TGCAGTTCAG CAGATGGTTT TTATGTTTCT TTCAAACCTG
    GCCTTATCTC ATGACTGTAA AGGAGTAATT CAGAAAAGTA ACTTCTTACA GAACTTTCTA
    TCTCTAACAT TGCCAAAAGG AGGAAATAAA CATCTCAGTA ATCTGACCAT TCTTTGGTTG
    AAGTTACTCC TGAATATATC ATTTGGAGAA GACGGGCAAC AAATGATCCT GAGGCTGGAT
    GGCTGTCTAG ACTTACTTAC AGAGATGAGC AAATATAAGC ATAAGAGCAG CCCTTATATA
    CCTCTTCTTA TCTTTCATAA TATTTGCTTC AGTCCTGCCA ATAAGCCCAA GATCCTGGCT
    AATGAAAAAG TCATTAGTGT GCTAGCTGCC TGTCTGGAAA GCGAGAATCA AAACGTTCAG
    AGGATTGGAG CAGCTGCACT TTGGGCTCTG ATTCACAATT ATCAAAAGGC AAAAATGACT
    TTGAAAAACC CATCCATAAA AAGAAGAGTG GATGAAGCAT ATTCCTTAGC AAAGAAAACT
    TTCTCAAACT CAGAAGAAAC CCCTCTAAAT GCCTATTACT TGAAATGTCT TGAAAATCTC
    GTTCAGCTTC TTAACTCTTC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285801
    Clone ID Related Accession (Same CDS sequence) XM_022412914.1
    Accession Version XM_022412914.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCCACAAC AACCTGAAAT CTTAATAGGA TATTTTCCTA AAGACAAAAG TAATCTCCAG 
    CAGATGGAAG TGTCTCCACG ACCAATGGTG AATCAGACTG TGAAGTGTTT GAAGTTTTCT
    ACGTTTCCTT GGCTACCCCT GACTACAACA GATAGACATG TTCTCTCCTC TAATGAAAGC
    TCTTTAAGAA GTAGTAACCA TACTTTAATT TGGAACACCT GTGAGCTACT GAAGGACGTT
    ATCATGCAAG ACTTTCCTGC TGAAATTTTC CTTCAAAGGC CAAAAATTGT TCAGAGCCTT
    TTATCTCTGT TGAAACTGGC CTTTGGTGGA GATGGAAGAC ATCGCCTGGC ATTACAGTCA
    GTGTCCTGCT TGCAGCAGCT GTGCACATAC TTAAGAAACA GACTTAACTT TCACCGAGAT
    CCAGGTTTTT TCTCCAGTAA AGAAGATACA GTTTCCCAGA ATTCTTCTTT GTCATACTGT
    CATGAAGTTA GAGTTATTCA TCATTCTCAG AATCCTTCTC TGGGAAGCAG TAGCCCCCGG
    CCTTCTGTGG TTGGACGCAC AGGCCAGCGA CCCAGAGGAG ATGGCCAGGA TTGGGATGCT
    GTGTCTTCCA GTGGAAGTAG TAGTCATGCT CCTGTGAACT CTAGGATCTC TGTGCATTCG
    CCTTTGGATG TGGTACACAT AGATCTGCCT GAGCTGGAGG CCGAAGACAC TCTGGAACTA
    CAATTCCAGC AGCTCAGTCT GCCCCAGTTC TGTGTCTCCG TTCTGCAATC AGCAGTTCCT
    CTCTTAAGAA CAGGTAGTAG ACAAATGATA ATAAGAGTTC TGGAATTGCT TGCAGAGGAT
    ATGATACTTA TTGGTGAAGC AATTTCAGCC GATATCTGGG ATGACAACAG CCTTTTTGCT
    GTAGATATGA AAGAAAAACT GCTTATGGTG TTGAGGTCCC TGGGAGATAC CATATGCTAC
    CATAAAAGTG GAGTGAGCCA GGACCAGCCA GAGTTAGTGC TGGTGCACCA CCGAATGGCC
    TTTGTCAGCG TTTCACTGTT TGCAGTCCGA GTCCTGCAGA TGCTTCTCCC TGTTGAAAAG
    GCAAGCCACC TTTTACCGGA GCCTATGTCA GCAGCATTAT TTCTAGTTTC TTTGGACATG
    CCTGTTTCTT TGGAATATCC AAATATTCAT GAAGCTGTTG TGGCATATTT GGAACAGTTG
    AATTCTGAAA ACTACAGTAT TTATAAACAA ACTGCAGAGG CTGTTTATTC AATTGAATGT
    ACCTGTAACT TCCTGTCTGA TGTTGGGAAG GAGGGCGAGA AGAATCTCTT GGAATTAGTA
    GAGCTGGCAG ACCAAGCCTT GAGAAGCTTT TCTTATCATC AGCATTTCCC CCTAATAAAG
    GAAATGATCT GCATGTGTTC AAAGATCTGC AAATCTGCGC AGGCCAGTCC ATTACTTCAA
    GGAGAAAGTC AGAAGGTGTT TCTGCGCATG TTATCTCACC CATTACTGCA AGTGAAAATT
    GAAACTTATA ACTGCTGTCT GGAAATTACT AAGGAATGTT TAGGTGTCTA TAACGTCACT
    AAACCTGTGT CTTCACTTTG TAACGGAATT CATTTTCTAC TTCACCCGAA AGTTCTGTAT
    GAAATCTCTG CATTTGGCAT TCAAGAGCCC AAAAATGAGG TGAATGCTGC TGCCAAGGCC
    ATTTTGCTGT ATCTGCTGCA GGGACGATTG ATGATGACAG CAGTGACCTG GAACAAGTTT
    ATTGAGTCTC TCTGTCCTGT CATTCCTGTG CTGCAGGGCT ATGCTGACAC AGAAGATCCT
    TTGGGTAACT CCATTCTCCT TCTGACGAAA GGGAATCCTG AGGCGGGCGA AGGGGCTCTC
    CCCTGTACCA CCCGACTGAA GTCCATGCTG CGGCTCCTGT TTGTCAGGAA GCCATTGGTC
    CGTTCACTAG CATTAAAGCT TTTAGCATGT CATCTCACAA GGGAAGAAGG GGCTGACACA
    AAGCGTCCTT TAATAGATGC CAGAATTCTC TCCAGAGTTA CTAATTTATT TATTGTGAAA
    AAACCTATTG AACTCACGTT GGATGACAGA AGAGAACTGA TTATTAGGTT GGAGACTGTT
    GAAAAGGTTT ATAAAATCTT TACCTCAGAT GATATTGATC TTGTTTTGAG AAAGTCAGCC
    GCAGAACAGT TAGCTGTGAT TATGCAAGAT ATTAAAATGC ATGCTGTGGT GAAAAAGTTA
    CACTTAGTTG ACACGATAAT TGAGTATCTA AATGAATGTG TAGGTCAGGA TGGTGAGCTC
    ATAGAATGTT TGATTCTGCC AAGCCTCACA CTCTTCAGGA AAGTTCTTTG TGCGGACCCG
    GTCATGCGCT TGGCCCTCTC ACAGCAGGCT TCTCTTTTGA CCCTGTTATT CAGAGTTTCC
    TTAATATTTC ATGAAAATTG TGCTGTCGTG ACTGAAGTCG GAGCATTATT TTGTCTTCTA
    TTATTTGATG AAGTATCAAG AATGGATATG TGGTCTGTTA ATCCTTCCAA TAAACCTTCT
    TCACCATCAG TCTTCAGTTT GCCTGTTTCA GTTTTTAGAA GGTATCATCT ACCAGTGCGT
    GTAGTTGGCC ACCATGCTGT GAGTCCTTAC TCGATAGTTT TGCCATTATC CACTGATTGT
    TTGGCCTTGA AACCAGTCTC AGACATGCTG AGAATAGCTT GGAACTTGTC CTGGTATCAT
    GGGAGTGAGA ATCTGTTGAA GCAAATGAAT TCCGAATCTA AAATCCAAGA ATTTTCAGAT
    ACATTGCAGT TAACCACAGA GGACACCCTC ACTCTGAAGA TGACACATAC AGCTAGTGGA
    CTGAAGGATT GTCTCCATTC CATTGTCGAG GCTGCAGCCC ACAGGGAAGT GAGGGCTGCT
    GTCACAAGAA TGAGTTTTTA TCTTCTGAAT GACAGACTGT CTTTAAAGGG TGGCTCTGGC
    CCATTTGGGG TTACCTTGAA GTCCTTGTCG TGGCATACCA CACTAAACAG GTTTTTACAG
    GTACTTCCTG CTTGCACTGA AGATGAGAAA CTGCTAATAG ATATCATACA TTTTTTAAAT
    AAGTTACTGA AGGAACAAAG AAAAAACTCA TCAGTGGAAC TTCTAAACTG GCTTCTAGAA
    TTACTTCTTA GACATAGTCC AAACCCACTG TTAGACCTGC TGGTTCTGAC GGAGTCACAG
    GCACGAGAGG AAACAGATGA CATCCAGACT GCTGTCAGGC AACAGCTCCA GAAAGAACTG
    ATTGCTCTTT TCAATACCTT GCTGCTTAGT TTCATGGCAG TTACTGACAG AAAATGCTTG
    GAACTTTTTT ATGTTTTCCA AACACAGCTG TCTCTGAAAC TGCTCCAGTG CCTGAGAGTC
    ACAGATGCTC CTCACTTCTA TGGCCTGCCA TCCCTTGAGA GGACCTTGCG AGGGATGGCT
    CATCTCACAG CATCTCCAGG ATGGAGCTCA CATTCTCCTC TCACAAAGCC ATTTGAAATT
    TGTGTTAAGT ACTTGTCAGG TCTACTTGAA GTCATTACTT CTTTTTATGT GGAGCGAGGA
    GGAAATGCTA TGTCCTTTAT GGGAAAAGGG GTCACTAAGA ACACAGTTCT TTGCTTGCTT
    CATTTATCCC ACGAGATGAT GGCCCAGGCC CAAAGTTCGG AGTGGATGTC ACTTTGGTTT
    TTGCCTTTGG GCAGTCACAG TGAAGGACAT GCTACCCCAC AAGGATTGGC GTGGCTCATT
    CCATTATGGG TTGACCGAGA CCCAGAGGTG AGGTTCACTT CATTGGGATT AGGATCAGCA
    CTGACCACCC TTGAAACTGG CTGTGTCGCG TTGGCAAATA GTTGTCAAAA CATTTCTGGT
    GGGCTCTGGG GGACAGTGGT GAACATTCTG CTGGACCAGT CGGAATGTAG TATGGTGCGC
    CGGGAGGCTG CATTTATTCT TCAGAATCTT CTTGTAATTC CAATGCCTTC AGTAATTATA
    AAGGATTATA CTTGGCAGGG TCCCTGTGTC CACGATGAGG ATTCTGGCCT GTCGCTCATA
    GGAAAACCTG CCCTTCAGGC TCTTCTATAT CATTGCCATT TCTATGAACA TTTGAATCAG
    ATGATAAAAC ACTGTTACCT GGGACGATAT ACATTTGATT TGAATTTTTC TGCTCTGGAT
    GGAACTTCAG AAGGTAGTGA TTTAAATGGT TTAGATGACT CATTCAAGTT TTGGAGAGCT
    CCATCTAGGA TGTCCAATCA AGATCAAGAT CCAAGTTCTC TCTCCACCTT GGAAACAATG
    GTGACACCTT CATCGGGGAG TACTGAATTC CCACCCTTTG GGCCCTCATC GGTGTCTCTC
    CCTGAGGCTT CACATGACCA GTTTGCGGCT CAAGTAATAG GTCCACATGA AACTGCATCA
    GCACAGCTTC CTCGTGACAC ATCCCTTCCT GCCCCTCTGC CCAAGCAGTG TGTGGTCACC
    CCACCTCTCC TTTCAGCAGT GTGCAGCCTT CTGGACGGCC TCTTGGTAAT TGCTCCAGGA
    GACACAGCAC GTGCTTTTCG GCAGGCCCGT CTCATAGAGC TTCTCTGTAG TATTATGGAT
    GCCACCCTCC TAGAGAGCTG TGTGCAGGAG CTGAAGGGTC CACTGCCTTT GTCATCTCCA
    GTTGAACACA CTCAGGCTCA GGTGTCCTTT CTCCTAGAAT ACCTGTCCTC TTTGTCTAGG
    CTTCTGCAGT CATGTTTACT GGTAGATCCT GACCTTGTGA TTGAGGATGA GCTTCTGAAA
    CCTCTGGTCA CAAACATCAT TGGGGTCCTC ACCATATGCA CCAAAGATGT GTTAGATGTA
    GAATTGATAT CTGCTTTTTA TCATACATGG ACACATTTAT TTAACCTTTT GGCTACGCTC
    CTGAGGAAAG CTGCTACTGC CTCTCTCTCT TCTATTATGA TGGGTCTGGC CAAGCACTGG
    GCAACAGTGA TTGATATGTT GTGCACATGT GTGAGTTTGT CTACCACATG CCCTACCTTA
    TCTACTGCCA GCCTGCAGTT CCTTTCTATT CTCTTGACTG AAGAAGCAAA AAGGCATCTC
    CAGGATAAGA ATAAAACAAA TTCATGCCAC ACTCCATCAA TGGCTTCACT TCTCGATAAA
    ACTCAAGAAA ATCAGAAATC TCTAGAACGA CTTAATGAAG TAATTCTTCA GTGCTATGAA
    GGAAAATCCT CCAAAGATGT CCTGAAAAGA GTTGCTGCAA ATACACTGAT GTCACTTCTG
    GCTGTCAGTA GAAGAGCACA GAGACAGGCT TTGAAAGCTG ATCTAATAGA CAGCTGCATG
    GAGCAGATGA AGCACATTAA TGCCCAGCTG AACCTAGATT CTCTTAGGCC TGGGAAAGCA
    ACCCTAAAGA AACAGGAGGA TGGTTTTATT AAAGAGTTAA GCATTGCCAT GCAGCTTCTA
    AGAAACTGTC TTTATCAAAA TGAAGAGTGT AAAGAAGCGG CTCTTGAATC TCATCTTGTT
    CCTGTCTTGC ACTCTCTCTG GCCTTGGCTT TTGATGGATG ATTCATTGAT GCAAATTGCT
    CTGCAGCTAC TTTGTGTCTA TACTGCAAAT TTTCCAAATG GTTGCAGTTC TCTTTGTTGG
    TCAAGTTACG GACAATATCC AGCTCAAGCT GCGTATAGAG GAGCCCCCAG CACCTCTCTG
    ATGGGGTGTA TCCTGAAGTT GGCTTCCCAG ATGCCTCTTG AGAACACTGC AGTTCAGCAG
    ATGGTTTTTA TGTTTCTTTC AAACCTGGCC TTATCTCATG ACTGTAAAGG AGTAATTCAG
    AAAAGTAACT TCTTACAGAA CTTTCTATCT CTAACATTGC CAAAAGGAGG AAATAAACAT
    CTCAGTAATC TGACCATTCT TTGGTTGAAG TTACTCCTGA ATATATCATT TGGAGAAGAC
    GGGCAACAAA TGATCCTGAG GCTGGATGGC TGTCTAGACT TACTTACAGA GATGAGCAAA
    TATAAGCATA AGAGCAGCCC TTATATACCT CTTCTTATCT TTCATAATAT TTGCTTCAGT
    CCTGCCAATA AGCCCAAGAT CCTGGCTAAT GAAAAAGTCA TTAGTGTGCT AGCTGCCTGT
    CTGGAAAGCG AGAATCAAAA CGTTCAGAGG ATTGGAGCAG CTGCACTTTG GGCTCTGATT
    CACAATTATC AAAAGGCAAA AATGACTTTG AAAAACCCAT CCATAAAAAG AAGAGTGGAT
    GAAGCATATT CCTTAGCAAA GAAAACTTTC TCAAACTCAG AAGAAACCCC TCTAAATGCC
    TATTACTTGA AATGTCTTGA AAATCTCGTT CAGCTTCTTA ACTCTTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268622.1
    CDS491..6781
    Translation

    Target ORF information:

    RefSeq Version XM_022412914.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412914.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGCCACAAC AACCTGAAAT CTTAATAGGA TATTTTCCTA AAGACAAAAG TAATCTCCAG 
    CAGATGGAAG TGTCTCCACG ACCAATGGTG AATCAGACTG TGAAGTGTTT GAAGTTTTCT
    ACGTTTCCTT GGCTACCCCT GACTACAACA GATAGACATG TTCTCTCCTC TAATGAAAGC
    TCTTTAAGAA GTAGTAACCA TACTTTAATT TGGAACACCT GTGAGCTACT GAAGGACGTT
    ATCATGCAAG ACTTTCCTGC TGAAATTTTC CTTCAAAGGC CAAAAATTGT TCAGAGCCTT
    TTATCTCTGT TGAAACTGGC CTTTGGTGGA GATGGAAGAC ATCGCCTGGC ATTACAGTCA
    GTGTCCTGCT TGCAGCAGCT GTGCACATAC TTAAGAAACA GACTTAACTT TCACCGAGAT
    CCAGGTTTTT TCTCCAGTAA AGAAGATACA GTTTCCCAGA ATTCTTCTTT GTCATACTGT
    CATGAAGTTA GAGTTATTCA TCATTCTCAG AATCCTTCTC TGGGAAGCAG TAGCCCCCGG
    CCTTCTGTGG TTGGACGCAC AGGCCAGCGA CCCAGAGGAG ATGGCCAGGA TTGGGATGCT
    GTGTCTTCCA GTGGAAGTAG TAGTCATGCT CCTGTGAACT CTAGGATCTC TGTGCATTCG
    CCTTTGGATG TGGTACACAT AGATCTGCCT GAGCTGGAGG CCGAAGACAC TCTGGAACTA
    CAATTCCAGC AGCTCAGTCT GCCCCAGTTC TGTGTCTCCG TTCTGCAATC AGCAGTTCCT
    CTCTTAAGAA CAGGTAGTAG ACAAATGATA ATAAGAGTTC TGGAATTGCT TGCAGAGGAT
    ATGATACTTA TTGGTGAAGC AATTTCAGCC GATATCTGGG ATGACAACAG CCTTTTTGCT
    GTAGATATGA AAGAAAAACT GCTTATGGTG TTGAGGTCCC TGGGAGATAC CATATGCTAC
    CATAAAAGTG GAGTGAGCCA GGACCAGCCA GAGTTAGTGC TGGTGCACCA CCGAATGGCC
    TTTGTCAGCG TTTCACTGTT TGCAGTCCGA GTCCTGCAGA TGCTTCTCCC TGTTGAAAAG
    GCAAGCCACC TTTTACCGGA GCCTATGTCA GCAGCATTAT TTCTAGTTTC TTTGGACATG
    CCTGTTTCTT TGGAATATCC AAATATTCAT GAAGCTGTTG TGGCATATTT GGAACAGTTG
    AATTCTGAAA ACTACAGTAT TTATAAACAA ACTGCAGAGG CTGTTTATTC AATTGAATGT
    ACCTGTAACT TCCTGTCTGA TGTTGGGAAG GAGGGCGAGA AGAATCTCTT GGAATTAGTA
    GAGCTGGCAG ACCAAGCCTT GAGAAGCTTT TCTTATCATC AGCATTTCCC CCTAATAAAG
    GAAATGATCT GCATGTGTTC AAAGATCTGC AAATCTGCGC AGGCCAGTCC ATTACTTCAA
    GGAGAAAGTC AGAAGGTGTT TCTGCGCATG TTATCTCACC CATTACTGCA AGTGAAAATT
    GAAACTTATA ACTGCTGTCT GGAAATTACT AAGGAATGTT TAGGTGTCTA TAACGTCACT
    AAACCTGTGT CTTCACTTTG TAACGGAATT CATTTTCTAC TTCACCCGAA AGTTCTGTAT
    GAAATCTCTG CATTTGGCAT TCAAGAGCCC AAAAATGAGG TGAATGCTGC TGCCAAGGCC
    ATTTTGCTGT ATCTGCTGCA GGGACGATTG ATGATGACAG CAGTGACCTG GAACAAGTTT
    ATTGAGTCTC TCTGTCCTGT CATTCCTGTG CTGCAGGGCT ATGCTGACAC AGAAGATCCT
    TTGGGTAACT CCATTCTCCT TCTGACGAAA GGGAATCCTG AGGCGGGCGA AGGGGCTCTC
    CCCTGTACCA CCCGACTGAA GTCCATGCTG CGGCTCCTGT TTGTCAGGAA GCCATTGGTC
    CGTTCACTAG CATTAAAGCT TTTAGCATGT CATCTCACAA GGGAAGAAGG GGCTGACACA
    AAGCGTCCTT TAATAGATGC CAGAATTCTC TCCAGAGTTA CTAATTTATT TATTGTGAAA
    AAACCTATTG AACTCACGTT GGATGACAGA AGAGAACTGA TTATTAGGTT GGAGACTGTT
    GAAAAGGTTT ATAAAATCTT TACCTCAGAT GATATTGATC TTGTTTTGAG AAAGTCAGCC
    GCAGAACAGT TAGCTGTGAT TATGCAAGAT ATTAAAATGC ATGCTGTGGT GAAAAAGTTA
    CACTTAGTTG ACACGATAAT TGAGTATCTA AATGAATGTG TAGGTCAGGA TGGTGAGCTC
    ATAGAATGTT TGATTCTGCC AAGCCTCACA CTCTTCAGGA AAGTTCTTTG TGCGGACCCG
    GTCATGCGCT TGGCCCTCTC ACAGCAGGCT TCTCTTTTGA CCCTGTTATT CAGAGTTTCC
    TTAATATTTC ATGAAAATTG TGCTGTCGTG ACTGAAGTCG GAGCATTATT TTGTCTTCTA
    TTATTTGATG AAGTATCAAG AATGGATATG TGGTCTGTTA ATCCTTCCAA TAAACCTTCT
    TCACCATCAG TCTTCAGTTT GCCTGTTTCA GTTTTTAGAA GGTATCATCT ACCAGTGCGT
    GTAGTTGGCC ACCATGCTGT GAGTCCTTAC TCGATAGTTT TGCCATTATC CACTGATTGT
    TTGGCCTTGA AACCAGTCTC AGACATGCTG AGAATAGCTT GGAACTTGTC CTGGTATCAT
    GGGAGTGAGA ATCTGTTGAA GCAAATGAAT TCCGAATCTA AAATCCAAGA ATTTTCAGAT
    ACATTGCAGT TAACCACAGA GGACACCCTC ACTCTGAAGA TGACACATAC AGCTAGTGGA
    CTGAAGGATT GTCTCCATTC CATTGTCGAG GCTGCAGCCC ACAGGGAAGT GAGGGCTGCT
    GTCACAAGAA TGAGTTTTTA TCTTCTGAAT GACAGACTGT CTTTAAAGGG TGGCTCTGGC
    CCATTTGGGG TTACCTTGAA GTCCTTGTCG TGGCATACCA CACTAAACAG GTTTTTACAG
    GTACTTCCTG CTTGCACTGA AGATGAGAAA CTGCTAATAG ATATCATACA TTTTTTAAAT
    AAGTTACTGA AGGAACAAAG AAAAAACTCA TCAGTGGAAC TTCTAAACTG GCTTCTAGAA
    TTACTTCTTA GACATAGTCC AAACCCACTG TTAGACCTGC TGGTTCTGAC GGAGTCACAG
    GCACGAGAGG AAACAGATGA CATCCAGACT GCTGTCAGGC AACAGCTCCA GAAAGAACTG
    ATTGCTCTTT TCAATACCTT GCTGCTTAGT TTCATGGCAG TTACTGACAG AAAATGCTTG
    GAACTTTTTT ATGTTTTCCA AACACAGCTG TCTCTGAAAC TGCTCCAGTG CCTGAGAGTC
    ACAGATGCTC CTCACTTCTA TGGCCTGCCA TCCCTTGAGA GGACCTTGCG AGGGATGGCT
    CATCTCACAG CATCTCCAGG ATGGAGCTCA CATTCTCCTC TCACAAAGCC ATTTGAAATT
    TGTGTTAAGT ACTTGTCAGG TCTACTTGAA GTCATTACTT CTTTTTATGT GGAGCGAGGA
    GGAAATGCTA TGTCCTTTAT GGGAAAAGGG GTCACTAAGA ACACAGTTCT TTGCTTGCTT
    CATTTATCCC ACGAGATGAT GGCCCAGGCC CAAAGTTCGG AGTGGATGTC ACTTTGGTTT
    TTGCCTTTGG GCAGTCACAG TGAAGGACAT GCTACCCCAC AAGGATTGGC GTGGCTCATT
    CCATTATGGG TTGACCGAGA CCCAGAGGTG AGGTTCACTT CATTGGGATT AGGATCAGCA
    CTGACCACCC TTGAAACTGG CTGTGTCGCG TTGGCAAATA GTTGTCAAAA CATTTCTGGT
    GGGCTCTGGG GGACAGTGGT GAACATTCTG CTGGACCAGT CGGAATGTAG TATGGTGCGC
    CGGGAGGCTG CATTTATTCT TCAGAATCTT CTTGTAATTC CAATGCCTTC AGTAATTATA
    AAGGATTATA CTTGGCAGGG TCCCTGTGTC CACGATGAGG ATTCTGGCCT GTCGCTCATA
    GGAAAACCTG CCCTTCAGGC TCTTCTATAT CATTGCCATT TCTATGAACA TTTGAATCAG
    ATGATAAAAC ACTGTTACCT GGGACGATAT ACATTTGATT TGAATTTTTC TGCTCTGGAT
    GGAACTTCAG AAGGTAGTGA TTTAAATGGT TTAGATGACT CATTCAAGTT TTGGAGAGCT
    CCATCTAGGA TGTCCAATCA AGATCAAGAT CCAAGTTCTC TCTCCACCTT GGAAACAATG
    GTGACACCTT CATCGGGGAG TACTGAATTC CCACCCTTTG GGCCCTCATC GGTGTCTCTC
    CCTGAGGCTT CACATGACCA GTTTGCGGCT CAAGTAATAG GTCCACATGA AACTGCATCA
    GCACAGCTTC CTCGTGACAC ATCCCTTCCT GCCCCTCTGC CCAAGCAGTG TGTGGTCACC
    CCACCTCTCC TTTCAGCAGT GTGCAGCCTT CTGGACGGCC TCTTGGTAAT TGCTCCAGGA
    GACACAGCAC GTGCTTTTCG GCAGGCCCGT CTCATAGAGC TTCTCTGTAG TATTATGGAT
    GCCACCCTCC TAGAGAGCTG TGTGCAGGAG CTGAAGGGTC CACTGCCTTT GTCATCTCCA
    GTTGAACACA CTCAGGCTCA GGTGTCCTTT CTCCTAGAAT ACCTGTCCTC TTTGTCTAGG
    CTTCTGCAGT CATGTTTACT GGTAGATCCT GACCTTGTGA TTGAGGATGA GCTTCTGAAA
    CCTCTGGTCA CAAACATCAT TGGGGTCCTC ACCATATGCA CCAAAGATGT GTTAGATGTA
    GAATTGATAT CTGCTTTTTA TCATACATGG ACACATTTAT TTAACCTTTT GGCTACGCTC
    CTGAGGAAAG CTGCTACTGC CTCTCTCTCT TCTATTATGA TGGGTCTGGC CAAGCACTGG
    GCAACAGTGA TTGATATGTT GTGCACATGT GTGAGTTTGT CTACCACATG CCCTACCTTA
    TCTACTGCCA GCCTGCAGTT CCTTTCTATT CTCTTGACTG AAGAAGCAAA AAGGCATCTC
    CAGGATAAGA ATAAAACAAA TTCATGCCAC ACTCCATCAA TGGCTTCACT TCTCGATAAA
    ACTCAAGAAA ATCAGAAATC TCTAGAACGA CTTAATGAAG TAATTCTTCA GTGCTATGAA
    GGAAAATCCT CCAAAGATGT CCTGAAAAGA GTTGCTGCAA ATACACTGAT GTCACTTCTG
    GCTGTCAGTA GAAGAGCACA GAGACAGGCT TTGAAAGCTG ATCTAATAGA CAGCTGCATG
    GAGCAGATGA AGCACATTAA TGCCCAGCTG AACCTAGATT CTCTTAGGCC TGGGAAAGCA
    ACCCTAAAGA AACAGGAGGA TGGTTTTATT AAAGAGTTAA GCATTGCCAT GCAGCTTCTA
    AGAAACTGTC TTTATCAAAA TGAAGAGTGT AAAGAAGCGG CTCTTGAATC TCATCTTGTT
    CCTGTCTTGC ACTCTCTCTG GCCTTGGCTT TTGATGGATG ATTCATTGAT GCAAATTGCT
    CTGCAGCTAC TTTGTGTCTA TACTGCAAAT TTTCCAAATG GTTGCAGTTC TCTTTGTTGG
    TCAAGTTACG GACAATATCC AGCTCAAGCT GCGTATAGAG GAGCCCCCAG CACCTCTCTG
    ATGGGGTGTA TCCTGAAGTT GGCTTCCCAG ATGCCTCTTG AGAACACTGC AGTTCAGCAG
    ATGGTTTTTA TGTTTCTTTC AAACCTGGCC TTATCTCATG ACTGTAAAGG AGTAATTCAG
    AAAAGTAACT TCTTACAGAA CTTTCTATCT CTAACATTGC CAAAAGGAGG AAATAAACAT
    CTCAGTAATC TGACCATTCT TTGGTTGAAG TTACTCCTGA ATATATCATT TGGAGAAGAC
    GGGCAACAAA TGATCCTGAG GCTGGATGGC TGTCTAGACT TACTTACAGA GATGAGCAAA
    TATAAGCATA AGAGCAGCCC TTATATACCT CTTCTTATCT TTCATAATAT TTGCTTCAGT
    CCTGCCAATA AGCCCAAGAT CCTGGCTAAT GAAAAAGTCA TTAGTGTGCT AGCTGCCTGT
    CTGGAAAGCG AGAATCAAAA CGTTCAGAGG ATTGGAGCAG CTGCACTTTG GGCTCTGATT
    CACAATTATC AAAAGGCAAA AATGACTTTG AAAAACCCAT CCATAAAAAG AAGAGTGGAT
    GAAGCATATT CCTTAGCAAA GAAAACTTTC TCAAACTCAG AAGAAACCCC TCTAAATGCC
    TATTACTTGA AATGTCTTGA AAATCTCGTT CAGCTTCTTA ACTCTTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285802
    Clone ID Related Accession (Same CDS sequence) XM_022412950.1
    Accession Version XM_022412950.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCAAGACT TTCCTGCTGA AATTTTCCTT CAAAGGCCAA AAATTGTTCA GAGCCTTTTA 
    TCTCTGTTGA AACTGGCCTT TGGTGGAGAT GGAAGACATC GCCTGGCATT ACAGTCAGTG
    TCCTGCTTGC AGCAGCTGTG CACATACTTA AGAAACAGAC TTAACTTTCA CCGAGATCCA
    GGTTTTTTCT CCAGTAAAGA AGATACAGTT TCCCAGAATT CTTCTTTGTC ATACTGTCAT
    GAAGTTAGAG TTATTCATCA TTCTCAGAAT CCTTCTCTGG GAAGCAGTAG CCCCCGGCCT
    TCTGTGGTTG GACGCACAGG CCAGCGACCC AGAGGAGATG GCCAGGATTG GGATGCTGTG
    TCTTCCAGTG GAAGTAGTAG TCATGCTCCT GTGAACTCTA GGATCTCTGT GCATTCGCCT
    TTGGATGTGG TACACATAGA TCTGCCTGAG CTGGAGGCCG AAGACACTCT GGAACTACAA
    TTCCAGCAGC TCAGTCTGCC CCAGTTCTGT GTCTCCGTTC TGCAATCAGC AGTTCCTCTC
    TTAAGAACAG GTAGTAGACA AATGATAATA AGAGTTCTGG AATTGCTTGC AGAGGATATG
    ATACTTATTG GTGAAGCAAT TTCAGCCGAT ATCTGGGATG ACAACAGCCT TTTTGCTGTA
    GATATGAAAG AAAAACTGCT TATGGTGTTG AGGTCCCTGG GAGATACCAT ATGCTACCAT
    AAAAGTGGAG TGAGCCAGGA CCAGCCAGAG TTAGTGCTGG TGCACCACCG AATGGCCTTT
    GTCAGCGTTT CACTGTTTGC AGTCCGAGTC CTGCAGATGC TTCTCCCTGT TGAAAAGGCA
    AGCCACCTTT TACCGGAGCC TATGTCAGCA GCATTATTTC TAGTTTCTTT GGACATGCCT
    GTTTCTTTGG AATATCCAAA TATTCATGAA GCTGTTGTGG CATATTTGGA ACAGTTGAAT
    TCTGAAAACT ACAGTATTTA TAAACAAACT GCAGAGGCTG TTTATTCAAT TGAATGTACC
    TGTAACTTCC TGTCTGATGT TGGGAAGGAG GGCGAGAAGA ATCTCTTGGA ATTAGTAGAG
    CTGGCAGACC AAGCCTTGAG AAGCTTTTCT TATCATCAGC ATTTCCCCCT AATAAAGGAA
    ATGATCTGCA TGTGTTCAAA GATCTGCAAA TCTGCGCAGG CCAGTCCATT ACTTCAAGGA
    GAAAGTCAGA AGGTGTTTCT GCGCATGTTA TCTCACCCAT TACTGCAAGT GAAAATTGAA
    ACTTATAACT GCTGTCTGGA AATTACTAAG GAATGTTTAG GTGTCTATAA CGTCACTAAA
    CCTGTGTCTT CACTTTGTAA CGGAATTCAT TTTCTACTTC ACCCGAAAGT TCTGTATGAA
    ATCTCTGCAT TTGGCATTCA AGAGCCCAAA AATGAGGTGA ATGCTGCTGC CAAGGCCATT
    TTGCTGTATC TGCTGCAGGG ACGATTGATG ATGACAGCAG TGACCTGGAA CAAGTTTATT
    GAGTCTCTCT GTCCTGTCAT TCCTGTGCTG CAGGGCTATG CTGACACAGA AGATCCTTTG
    GGTAACTCCA TTCTCCTTCT GACGAAAGGG AATCCTGAGG CGGGCGAAGG GGCTCTCCCC
    TGTACCACCC GACTGAAGTC CATGCTGCGG CTCCTGTTTG TCAGGAAGCC ATTGGTCCGT
    TCACTAGCAT TAAAGCTTTT AGCATGTCAT CTCACAAGGG AAGAAGGGGC TGACACAAAG
    CGTCCTTTAA TAGATGCCAG AATTCTCTCC AGAGTTACTA ATTTATTTAT TGTGAAAAAA
    CCTATTGAAC TCACGTTGGA TGACAGAAGA GAACTGATTA TTAGGTTGGA GACTGTTGAA
    AAGGTTTATA AAATCTTTAC CTCAGATGAT ATTGATCTTG TTTTGAGAAA GTCAGCCGCA
    GAACAGTTAG CTGTGATTAT GCAAGATATT AAAATGCATG CTGTGGTGAA AAAGTTACAC
    TTAGTTGACA CGATAATTGA GTATCTAAAT GAATGTGTAG GTCAGGATGG TGAGCTCATA
    GAATGTTTGA TTCTGCCAAG CCTCACACTC TTCAGGAAAG TTCTTTGTGC GGACCCGGTC
    ATGCGCTTGG CCCTCTCACA GCAGGCTTCT CTTTTGACCC TGTTATTCAG AGTTTCCTTA
    ATATTTCATG AAAATTGTGC TGTCGTGACT GAAGTCGGAG CATTATTTTG TCTTCTATTA
    TTTGATGAAG TATCAAGAAT GGATATGTGG TCTGTTAATC CTTCCAATAA ACCTTCTTCA
    CCATCAGTCT TCAGTTTGCC TGTTTCAGTT TTTAGAAGGT ATCATCTACC AGTGCGTGTA
    GTTGGCCACC ATGCTGTGAG TCCTTACTCG ATAGTTTTGC CATTATCCAC TGATTGTTTG
    GCCTTGAAAC CAGTCTCAGA CATGCTGAGA ATAGCTTGGA ACTTGTCCTG GTATCATGGG
    AGTGAGAATC TGTTGAAGCA AATGAATTCC GAATCTAAAA TCCAAGAATT TTCAGATACA
    TTGCAGTTAA CCACAGAGGA CACCCTCACT CTGAAGATGA CACATACAGC TAGTGGACTG
    AAGGATTGTC TCCATTCCAT TGTCGAGGCT GCAGCCCACA GGGAAGTGAG GGCTGCTGTC
    ACAAGAATGA GTTTTTATCT TCTGAATGAC AGACTGTCTT TAAAGGGTGG CTCTGGCCCA
    TTTGGGGTTA CCTTGAAGTC CTTGTCGTGG CATACCACAC TAAACAGGTT TTTACAGGTA
    CTTCCTGCTT GCACTGAAGA TGAGAAACTG CTAATAGATA TCATACATTT TTTAAATAAG
    TTACTGAAGG AACAAAGAAA AAACTCATCA GTGGAACTTC TAAACTGGCT TCTAGAATTA
    CTTCTTAGAC ATAGTCCAAA CCCACTGTTA GACCTGCTGG TTCTGACGGA GTCACAGGCA
    CGAGAGGAAA CAGATGACAT CCAGACTGCT GTCAGGCAAC AGCTCCAGAA AGAACTGATT
    GCTCTTTTCA ATACCTTGCT GCTTAGTTTC ATGGCAGTTA CTGACAGAAA ATGCTTGGAA
    CTTTTTTATG TTTTCCAAAC ACAGCTGTCT CTGAAACTGC TCCAGTGCCT GAGAGTCACA
    GATGCTCCTC ACTTCTATGG CCTGCCATCC CTTGAGAGGA CCTTGCGAGG GATGGCTCAT
    CTCACAGCAT CTCCAGGATG GAGCTCACAT TCTCCTCTCA CAAAGCCATT TGAAATTTGT
    GTTAAGTACT TGTCAGGTCT ACTTGAAGTC ATTACTTCTT TTTATGTGGA GCGAGGAGGA
    AATGCTATGT CCTTTATGGG AAAAGGGGTC ACTAAGAACA CAGTTCTTTG CTTGCTTCAT
    TTATCCCACG AGATGATGGC CCAGGCCCAA AGTTCGGAGT GGATGTCACT TTGGTTTTTG
    CCTTTGGGCA GTCACAGTGA AGGACATGCT ACCCCACAAG GATTGGCGTG GCTCATTCCA
    TTATGGGTTG ACCGAGACCC AGAGGTGAGG TTCACTTCAT TGGGATTAGG ATCAGCACTG
    ACCACCCTTG AAACTGGCTG TGTCGCGTTG GCAAATAGTT GTCAAAACAT TTCTGGTGGG
    CTCTGGGGGA CAGTGGTGAA CATTCTGCTG GACCAGTCGG AATGTAGTAT GGTGCGCCGG
    GAGGCTGCAT TTATTCTTCA GAATCTTCTT GTAATTCCAA TGCCTTCAGT AATTATAAAG
    GATTATACTT GGCAGGGTCC CTGTGTCCAC GATGAGGATT CTGGCCTGTC GCTCATAGGA
    AAACCTGCCC TTCAGGCTCT TCTATATCAT TGCCATTTCT ATGAACATTT GAATCAGATG
    ATAAAACACT GTTACCTGGG ACGATATACA TTTGATTTGA ATTTTTCTGC TCTGGATGGA
    ACTTCAGAAG GTAGTGATTT AAATGGTTTA GATGACTCAT TCAAGTTTTG GAGAGCTCCA
    TCTAGGATGT CCAATCAAGA TCAAGATCCA AGTTCTCTCT CCACCTTGGA AACAATGGTG
    ACACCTTCAT CGGGGAGTAC TGAATTCCCA CCCTTTGGGC CCTCATCGGT GTCTCTCCCT
    GAGGCTTCAC ATGACCAGTT TGCGGCTCAA GTAATAGGTC CACATGAAAC TGCATCAGCA
    CAGCTTCCTC GTGACACATC CCTTCCTGCC CCTCTGCCCA AGCAGTGTGT GGTCACCCCA
    CCTCTCCTTT CAGCAGTGTG CAGCCTTCTG GACGGCCTCT TGGTAATTGC TCCAGGAGAC
    ACAGCACGTG CTTTTCGGCA GGCCCGTCTC ATAGAGCTTC TCTGTAGTAT TATGGATGCC
    ACCCTCCTAG AGAGCTGTGT GCAGGAGCTG AAGGGTCCAC TGCCTTTGTC ATCTCCAGTT
    GAACACACTC AGGCTCAGGT GTCCTTTCTC CTAGAATACC TGTCCTCTTT GTCTAGGCTT
    CTGCAGTCAT GTTTACTGGT AGATCCTGAC CTTGTGATTG AGGATGAGCT TCTGAAACCT
    CTGGTCACAA ACATCATTGG GGTCCTCACC ATATGCACCA AAGATGTGTT AGATGTAGAA
    TTGATATCTG CTTTTTATCA TACATGGACA CATTTATTTA ACCTTTTGGC TACGCTCCTG
    AGGAAAGCTG CTACTGCCTC TCTCTCTTCT ATTATGATGG GTCTGGCCAA GCACTGGGCA
    ACAGTGATTG ATATGTTGTG CACATGTGTG AGTTTGTCTA CCACATGCCC TACCTTATCT
    ACTGCCAGCC TGCAGTTCCT TTCTATTCTC TTGACTGAAG AAGCAAAAAG GCATCTCCAG
    GATAAGAATA AAACAAATTC ATGCCACACT CCATCAATGG CTTCACTTCT CGATAAAACT
    CAAGAAAATC AGAAATCTCT AGAACGACTT AATGAAGTAA TTCTTCAGTG CTATGAAGGA
    AAATCCTCCA AAGATGTCCT GAAAAGAGTT GCTGCAAATA CACTGATGTC ACTTCTGGCT
    GTCAGTAGAA GAGCACAGAG ACAGGCTTTG AAAGCTGATC TAATAGACAG CTGCATGGAG
    CAGATGAAGC ACATTAATGC CCAGCTGAAC CTAGATTCTC TTAGGCCTGG GAAAGCAACC
    CTAAAGAAAC AGGAGGATGG TTTTATTAAA GAGTTAAGCA TTGCCATGCA GCTTCTAAGA
    AACTGTCTTT ATCAAAATGA AGAGTGTAAA GAAGCGGCTC TTGAATCTCA TCTTGTTCCT
    GTCTTGCACT CTCTCTGGCC TTGGCTTTTG ATGGATGATT CATTGATGCA AATTGCTCTG
    CAGCTACTTT GTGTCTATAC TGCAAATTTT CCAAATGGTT GCAGTTCTCT TTGTTGGTCA
    AGTTACGGAC AATATCCAGC TCAAGCTGCG TATAGAGGAG CCCCCAGCAC CTCTCTGATG
    GGGTGTATCC TGAAGTTGGC TTCCCAGATG CCTCTTGAGA ACACTGCAGT TCAGCAGATG
    GTTTTTATGT TTCTTTCAAA CCTGGCCTTA TCTCATGACT GTAAAGGAGT AATTCAGAAA
    AGTAACTTCT TACAGAACTT TCTATCTCTA ACATTGCCAA AAGGAGGAAA TAAACATCTC
    AGTAATCTGA CCATTCTTTG GTTGAAGTTA CTCCTGAATA TATCATTTGG AGAAGACGGG
    CAACAAATGA TCCTGAGGCT GGATGGCTGT CTAGACTTAC TTACAGAGAT GAGCAAATAT
    AAGCATAAGA GCAGCCCTTA TATACCTCTT CTTATCTTTC ATAATATTTG CTTCAGTCCT
    GCCAATAAGC CCAAGATCCT GGCTAATGAA AAAGTCATTA GTGTGCTAGC TGCCTGTCTG
    GAAAGCGAGA ATCAAAACGT TCAGAGGATT GGAGCAGCTG CACTTTGGGC TCTGATTCAC
    AATTATCAAA AGGCAAAAAT GACTTTGAAA AACCCATCCA TAAAAAGAAG AGTGGATGAA
    GCATATTCCT TAGCAAAGAA AACTTTCTCA AACTCAGAAG AAACCCCTCT AAATGCCTAT
    TACTTGAAAT GTCTTGAAAA TCTCGTTCAG CTTCTTAACT CTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022268658.1
    CDS179..6226
    Translation

    Target ORF information:

    RefSeq Version XM_022412950.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022412950.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGCAAGACT TTCCTGCTGA AATTTTCCTT CAAAGGCCAA AAATTGTTCA GAGCCTTTTA 
    TCTCTGTTGA AACTGGCCTT TGGTGGAGAT GGAAGACATC GCCTGGCATT ACAGTCAGTG
    TCCTGCTTGC AGCAGCTGTG CACATACTTA AGAAACAGAC TTAACTTTCA CCGAGATCCA
    GGTTTTTTCT CCAGTAAAGA AGATACAGTT TCCCAGAATT CTTCTTTGTC ATACTGTCAT
    GAAGTTAGAG TTATTCATCA TTCTCAGAAT CCTTCTCTGG GAAGCAGTAG CCCCCGGCCT
    TCTGTGGTTG GACGCACAGG CCAGCGACCC AGAGGAGATG GCCAGGATTG GGATGCTGTG
    TCTTCCAGTG GAAGTAGTAG TCATGCTCCT GTGAACTCTA GGATCTCTGT GCATTCGCCT
    TTGGATGTGG TACACATAGA TCTGCCTGAG CTGGAGGCCG AAGACACTCT GGAACTACAA
    TTCCAGCAGC TCAGTCTGCC CCAGTTCTGT GTCTCCGTTC TGCAATCAGC AGTTCCTCTC
    TTAAGAACAG GTAGTAGACA AATGATAATA AGAGTTCTGG AATTGCTTGC AGAGGATATG
    ATACTTATTG GTGAAGCAAT TTCAGCCGAT ATCTGGGATG ACAACAGCCT TTTTGCTGTA
    GATATGAAAG AAAAACTGCT TATGGTGTTG AGGTCCCTGG GAGATACCAT ATGCTACCAT
    AAAAGTGGAG TGAGCCAGGA CCAGCCAGAG TTAGTGCTGG TGCACCACCG AATGGCCTTT
    GTCAGCGTTT CACTGTTTGC AGTCCGAGTC CTGCAGATGC TTCTCCCTGT TGAAAAGGCA
    AGCCACCTTT TACCGGAGCC TATGTCAGCA GCATTATTTC TAGTTTCTTT GGACATGCCT
    GTTTCTTTGG AATATCCAAA TATTCATGAA GCTGTTGTGG CATATTTGGA ACAGTTGAAT
    TCTGAAAACT ACAGTATTTA TAAACAAACT GCAGAGGCTG TTTATTCAAT TGAATGTACC
    TGTAACTTCC TGTCTGATGT TGGGAAGGAG GGCGAGAAGA ATCTCTTGGA ATTAGTAGAG
    CTGGCAGACC AAGCCTTGAG AAGCTTTTCT TATCATCAGC ATTTCCCCCT AATAAAGGAA
    ATGATCTGCA TGTGTTCAAA GATCTGCAAA TCTGCGCAGG CCAGTCCATT ACTTCAAGGA
    GAAAGTCAGA AGGTGTTTCT GCGCATGTTA TCTCACCCAT TACTGCAAGT GAAAATTGAA
    ACTTATAACT GCTGTCTGGA AATTACTAAG GAATGTTTAG GTGTCTATAA CGTCACTAAA
    CCTGTGTCTT CACTTTGTAA CGGAATTCAT TTTCTACTTC ACCCGAAAGT TCTGTATGAA
    ATCTCTGCAT TTGGCATTCA AGAGCCCAAA AATGAGGTGA ATGCTGCTGC CAAGGCCATT
    TTGCTGTATC TGCTGCAGGG ACGATTGATG ATGACAGCAG TGACCTGGAA CAAGTTTATT
    GAGTCTCTCT GTCCTGTCAT TCCTGTGCTG CAGGGCTATG CTGACACAGA AGATCCTTTG
    GGTAACTCCA TTCTCCTTCT GACGAAAGGG AATCCTGAGG CGGGCGAAGG GGCTCTCCCC
    TGTACCACCC GACTGAAGTC CATGCTGCGG CTCCTGTTTG TCAGGAAGCC ATTGGTCCGT
    TCACTAGCAT TAAAGCTTTT AGCATGTCAT CTCACAAGGG AAGAAGGGGC TGACACAAAG
    CGTCCTTTAA TAGATGCCAG AATTCTCTCC AGAGTTACTA ATTTATTTAT TGTGAAAAAA
    CCTATTGAAC TCACGTTGGA TGACAGAAGA GAACTGATTA TTAGGTTGGA GACTGTTGAA
    AAGGTTTATA AAATCTTTAC CTCAGATGAT ATTGATCTTG TTTTGAGAAA GTCAGCCGCA
    GAACAGTTAG CTGTGATTAT GCAAGATATT AAAATGCATG CTGTGGTGAA AAAGTTACAC
    TTAGTTGACA CGATAATTGA GTATCTAAAT GAATGTGTAG GTCAGGATGG TGAGCTCATA
    GAATGTTTGA TTCTGCCAAG CCTCACACTC TTCAGGAAAG TTCTTTGTGC GGACCCGGTC
    ATGCGCTTGG CCCTCTCACA GCAGGCTTCT CTTTTGACCC TGTTATTCAG AGTTTCCTTA
    ATATTTCATG AAAATTGTGC TGTCGTGACT GAAGTCGGAG CATTATTTTG TCTTCTATTA
    TTTGATGAAG TATCAAGAAT GGATATGTGG TCTGTTAATC CTTCCAATAA ACCTTCTTCA
    CCATCAGTCT TCAGTTTGCC TGTTTCAGTT TTTAGAAGGT ATCATCTACC AGTGCGTGTA
    GTTGGCCACC ATGCTGTGAG TCCTTACTCG ATAGTTTTGC CATTATCCAC TGATTGTTTG
    GCCTTGAAAC CAGTCTCAGA CATGCTGAGA ATAGCTTGGA ACTTGTCCTG GTATCATGGG
    AGTGAGAATC TGTTGAAGCA AATGAATTCC GAATCTAAAA TCCAAGAATT TTCAGATACA
    TTGCAGTTAA CCACAGAGGA CACCCTCACT CTGAAGATGA CACATACAGC TAGTGGACTG
    AAGGATTGTC TCCATTCCAT TGTCGAGGCT GCAGCCCACA GGGAAGTGAG GGCTGCTGTC
    ACAAGAATGA GTTTTTATCT TCTGAATGAC AGACTGTCTT TAAAGGGTGG CTCTGGCCCA
    TTTGGGGTTA CCTTGAAGTC CTTGTCGTGG CATACCACAC TAAACAGGTT TTTACAGGTA
    CTTCCTGCTT GCACTGAAGA TGAGAAACTG CTAATAGATA TCATACATTT TTTAAATAAG
    TTACTGAAGG AACAAAGAAA AAACTCATCA GTGGAACTTC TAAACTGGCT TCTAGAATTA
    CTTCTTAGAC ATAGTCCAAA CCCACTGTTA GACCTGCTGG TTCTGACGGA GTCACAGGCA
    CGAGAGGAAA CAGATGACAT CCAGACTGCT GTCAGGCAAC AGCTCCAGAA AGAACTGATT
    GCTCTTTTCA ATACCTTGCT GCTTAGTTTC ATGGCAGTTA CTGACAGAAA ATGCTTGGAA
    CTTTTTTATG TTTTCCAAAC ACAGCTGTCT CTGAAACTGC TCCAGTGCCT GAGAGTCACA
    GATGCTCCTC ACTTCTATGG CCTGCCATCC CTTGAGAGGA CCTTGCGAGG GATGGCTCAT
    CTCACAGCAT CTCCAGGATG GAGCTCACAT TCTCCTCTCA CAAAGCCATT TGAAATTTGT
    GTTAAGTACT TGTCAGGTCT ACTTGAAGTC ATTACTTCTT TTTATGTGGA GCGAGGAGGA
    AATGCTATGT CCTTTATGGG AAAAGGGGTC ACTAAGAACA CAGTTCTTTG CTTGCTTCAT
    TTATCCCACG AGATGATGGC CCAGGCCCAA AGTTCGGAGT GGATGTCACT TTGGTTTTTG
    CCTTTGGGCA GTCACAGTGA AGGACATGCT ACCCCACAAG GATTGGCGTG GCTCATTCCA
    TTATGGGTTG ACCGAGACCC AGAGGTGAGG TTCACTTCAT TGGGATTAGG ATCAGCACTG
    ACCACCCTTG AAACTGGCTG TGTCGCGTTG GCAAATAGTT GTCAAAACAT TTCTGGTGGG
    CTCTGGGGGA CAGTGGTGAA CATTCTGCTG GACCAGTCGG AATGTAGTAT GGTGCGCCGG
    GAGGCTGCAT TTATTCTTCA GAATCTTCTT GTAATTCCAA TGCCTTCAGT AATTATAAAG
    GATTATACTT GGCAGGGTCC CTGTGTCCAC GATGAGGATT CTGGCCTGTC GCTCATAGGA
    AAACCTGCCC TTCAGGCTCT TCTATATCAT TGCCATTTCT ATGAACATTT GAATCAGATG
    ATAAAACACT GTTACCTGGG ACGATATACA TTTGATTTGA ATTTTTCTGC TCTGGATGGA
    ACTTCAGAAG GTAGTGATTT AAATGGTTTA GATGACTCAT TCAAGTTTTG GAGAGCTCCA
    TCTAGGATGT CCAATCAAGA TCAAGATCCA AGTTCTCTCT CCACCTTGGA AACAATGGTG
    ACACCTTCAT CGGGGAGTAC TGAATTCCCA CCCTTTGGGC CCTCATCGGT GTCTCTCCCT
    GAGGCTTCAC ATGACCAGTT TGCGGCTCAA GTAATAGGTC CACATGAAAC TGCATCAGCA
    CAGCTTCCTC GTGACACATC CCTTCCTGCC CCTCTGCCCA AGCAGTGTGT GGTCACCCCA
    CCTCTCCTTT CAGCAGTGTG CAGCCTTCTG GACGGCCTCT TGGTAATTGC TCCAGGAGAC
    ACAGCACGTG CTTTTCGGCA GGCCCGTCTC ATAGAGCTTC TCTGTAGTAT TATGGATGCC
    ACCCTCCTAG AGAGCTGTGT GCAGGAGCTG AAGGGTCCAC TGCCTTTGTC ATCTCCAGTT
    GAACACACTC AGGCTCAGGT GTCCTTTCTC CTAGAATACC TGTCCTCTTT GTCTAGGCTT
    CTGCAGTCAT GTTTACTGGT AGATCCTGAC CTTGTGATTG AGGATGAGCT TCTGAAACCT
    CTGGTCACAA ACATCATTGG GGTCCTCACC ATATGCACCA AAGATGTGTT AGATGTAGAA
    TTGATATCTG CTTTTTATCA TACATGGACA CATTTATTTA ACCTTTTGGC TACGCTCCTG
    AGGAAAGCTG CTACTGCCTC TCTCTCTTCT ATTATGATGG GTCTGGCCAA GCACTGGGCA
    ACAGTGATTG ATATGTTGTG CACATGTGTG AGTTTGTCTA CCACATGCCC TACCTTATCT
    ACTGCCAGCC TGCAGTTCCT TTCTATTCTC TTGACTGAAG AAGCAAAAAG GCATCTCCAG
    GATAAGAATA AAACAAATTC ATGCCACACT CCATCAATGG CTTCACTTCT CGATAAAACT
    CAAGAAAATC AGAAATCTCT AGAACGACTT AATGAAGTAA TTCTTCAGTG CTATGAAGGA
    AAATCCTCCA AAGATGTCCT GAAAAGAGTT GCTGCAAATA CACTGATGTC ACTTCTGGCT
    GTCAGTAGAA GAGCACAGAG ACAGGCTTTG AAAGCTGATC TAATAGACAG CTGCATGGAG
    CAGATGAAGC ACATTAATGC CCAGCTGAAC CTAGATTCTC TTAGGCCTGG GAAAGCAACC
    CTAAAGAAAC AGGAGGATGG TTTTATTAAA GAGTTAAGCA TTGCCATGCA GCTTCTAAGA
    AACTGTCTTT ATCAAAATGA AGAGTGTAAA GAAGCGGCTC TTGAATCTCA TCTTGTTCCT
    GTCTTGCACT CTCTCTGGCC TTGGCTTTTG ATGGATGATT CATTGATGCA AATTGCTCTG
    CAGCTACTTT GTGTCTATAC TGCAAATTTT CCAAATGGTT GCAGTTCTCT TTGTTGGTCA
    AGTTACGGAC AATATCCAGC TCAAGCTGCG TATAGAGGAG CCCCCAGCAC CTCTCTGATG
    GGGTGTATCC TGAAGTTGGC TTCCCAGATG CCTCTTGAGA ACACTGCAGT TCAGCAGATG
    GTTTTTATGT TTCTTTCAAA CCTGGCCTTA TCTCATGACT GTAAAGGAGT AATTCAGAAA
    AGTAACTTCT TACAGAACTT TCTATCTCTA ACATTGCCAA AAGGAGGAAA TAAACATCTC
    AGTAATCTGA CCATTCTTTG GTTGAAGTTA CTCCTGAATA TATCATTTGG AGAAGACGGG
    CAACAAATGA TCCTGAGGCT GGATGGCTGT CTAGACTTAC TTACAGAGAT GAGCAAATAT
    AAGCATAAGA GCAGCCCTTA TATACCTCTT CTTATCTTTC ATAATATTTG CTTCAGTCCT
    GCCAATAAGC CCAAGATCCT GGCTAATGAA AAAGTCATTA GTGTGCTAGC TGCCTGTCTG
    GAAAGCGAGA ATCAAAACGT TCAGAGGATT GGAGCAGCTG CACTTTGGGC TCTGATTCAC
    AATTATCAAA AGGCAAAAAT GACTTTGAAA AACCCATCCA TAAAAAGAAG AGTGGATGAA
    GCATATTCCT TAGCAAAGAA AACTTTCTCA AACTCAGAAG AAACCCCTCT AAATGCCTAT
    TACTTGAAAT GTCTTGAAAA TCTCGTTCAG CTTCTTAACT CTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00713
    Clone ID Related Accession (Same CDS sequence) XM_005615328.2 , XM_005615328.3
    Accession Version XM_005615328.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rotatin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTTTA
    GATGACTCAT TCAAGTTTTG GAGAGCTCCA TCTAGGATGT CCAATCAAGA TCAAGATCCA
    AGTTCTCTCT CCACCTTGGA AACAATGGTG ACACCTTCAT CGGGGAGTAC TGAATTCCCA
    CCCTTTGGGC CCTCATCGGT GTCTCTCCCT GAGGCTTCAC ATGACCAGTT TGCGGCTCAA
    GTAATAGGTC CACATGAAAC TGCATCAGCA CAGCTTCCTC GTGACACATC CCTTCCTGCC
    CCTCTGCCCA AGCAGTGTGT GGTCACCCCA CCTCTCCTTT CAGCAGTGTG CAGCCTTCTG
    GACGGCCTCT TGGTAATTGC TCCAGGAGAC ACAGCACGTG CTTTTCGGCA GGCCCGTCTC
    ATAGAGCTTC TCTGTAGTAT TATGGATGCC ACCCTCCTAG AGAGCTGTGT GCAGGAGCTG
    AAGGGTCCAC TGCCTTTGTC ATCTCCAGTT GAACACACTC AGGCTCAGGT GTCCTTTCTC
    CTAGAATACC TGTCCTCTTT GTCTAGGCTT CTGCAGTCAT GTTTACTGGT AGATCCTGAC
    CTTGTGATTG AGGATGAGCT TCTGAAACCT CTGGTCACAA ACATCATTGG GGTCCTCACC
    ATATGCACCA AAGATGTGTT AGATGTAGAA TTGATATCTG CTTTTTATCA TACATGGACA
    CATTTATTTA ACCTTTTGGC TACGCTCCTG AGGAAAGCTG CTACTGCCTC TCTCTCTTCT
    ATTATGATGG GTCTGGCCAA GCACTGGGCA ACAGTGATTG ATATGTTGTG CACATGTGTG
    AGTTTGTCTA CCACATGCCC TACCTTATCT ACTGCCAGCC TGCAGTTCCT TTCTATTCTC
    TTGACTGAAG AAGCAAAAAG GCATCTCCAG GATAAGAATA AAACAAATTC ATGCCACACT
    CCATCAATGG CTTCACTTCT CGATAAAACT CAAGAAAATC AGAAATCTCT AGAACGACTT
    AATGAAGTAA TTCTTCAGTG CTATGAAGGA AAATCCTCCA AAGATGTCCT GAAAAGAGTT
    GCTGCAAATA CACTGATGTC ACTTCTGGCT GTCAGTAGAA GAGCACAGAG ACAGGCTTTG
    AAAGCTGATC TAATAGACAG CTGCATGGAG CAGATGAAGC ACATTAATGC CCAGCTGAAC
    CTAGATTCTC TTAGGCCTGG GAAAGCAACC CTAAAGAAAC AGGAGGATGG TTTTATTAAA
    GAGTTAAGCA TTGCCATGCA GCTTCTAAGA AACTGTCTTT ATCAAAATGA AGAGTGTAAA
    GAAGCGGCTC TTGAATCTCA TCTTGTTCCT GTCTTGCACT CTCTCTGGCC TTGGCTTTTG
    ATGGATGATT CATTGATGCA AATTGCTCTG CAGCTACTTT GTGTCTATAC TGCAAATTTT
    CCAAATGGTT GCAGTTCTCT TTGTTGGTCA AGTTACGGAC AATATCCAGC TCAAGCTGCG
    TATAGAGGAG CCCCCAGCAC CTCTCTGATG GGGTGTATCC TGAAGTTGGC TTCCCAGATG
    CCTCTTGAGA ACACTGCAGT TCAGCAGATG GTTTTTATGT TTCTTTCAAA CCTGGCCTTA
    TCTCATGACT GTAAAGGAGT AATTCAGAAA AGTAACTTCT TACAGAACTT TCTATCTCTA
    ACATTGCCAA AAGGAGGAAA TAAACATCTC AGTAATCTGA CCATTCTTTG GTTGAAGTTA
    CTCCTGAATA TATCATTTGG AGAAGACGGG CAACAAATGA TCCTGAGGCT GGATGGCTGT
    CTAGACTTAC TTACAGAGAT GAGCAAATAT AAGCATAAGA GCAGCCCTTA TATACCTCTT
    CTTATCTTTC ATAATATTTG CTTCAGTCCT GCCAATAAGC CCAAGATCCT GGCTAATGAA
    AAAGTCATTA GTGTGCTAGC TGCCTGTCTG GAAAGCGAGA ATCAAAACGT TCAGAGGATT
    GGAGCAGCTG CACTTTGGGC TCTGATTCAC AATTATCAAA AGGCAAAAAT GACTTTGAAA
    AACCCATCCA TAAAAAGAAG AGTGGATGAA GCATATTCCT TAGCAAAGAA AACTTTCTCA
    AACTCAGAAG AAACCCCTCT AAATGCCTAT TACTTGAAAT GTCTTGAAAA TCTCGTTCAG
    CTTCTTAACT CTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615385.1
    CDS343..7020
    Translation

    Target ORF information:

    RefSeq Version XM_005615328.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTTTA
    GATGACTCAT TCAAGTTTTG GAGAGCTCCA TCTAGGATGT CCAATCAAGA TCAAGATCCA
    AGTTCTCTCT CCACCTTGGA AACAATGGTG ACACCTTCAT CGGGGAGTAC TGAATTCCCA
    CCCTTTGGGC CCTCATCGGT GTCTCTCCCT GAGGCTTCAC ATGACCAGTT TGCGGCTCAA
    GTAATAGGTC CACATGAAAC TGCATCAGCA CAGCTTCCTC GTGACACATC CCTTCCTGCC
    CCTCTGCCCA AGCAGTGTGT GGTCACCCCA CCTCTCCTTT CAGCAGTGTG CAGCCTTCTG
    GACGGCCTCT TGGTAATTGC TCCAGGAGAC ACAGCACGTG CTTTTCGGCA GGCCCGTCTC
    ATAGAGCTTC TCTGTAGTAT TATGGATGCC ACCCTCCTAG AGAGCTGTGT GCAGGAGCTG
    AAGGGTCCAC TGCCTTTGTC ATCTCCAGTT GAACACACTC AGGCTCAGGT GTCCTTTCTC
    CTAGAATACC TGTCCTCTTT GTCTAGGCTT CTGCAGTCAT GTTTACTGGT AGATCCTGAC
    CTTGTGATTG AGGATGAGCT TCTGAAACCT CTGGTCACAA ACATCATTGG GGTCCTCACC
    ATATGCACCA AAGATGTGTT AGATGTAGAA TTGATATCTG CTTTTTATCA TACATGGACA
    CATTTATTTA ACCTTTTGGC TACGCTCCTG AGGAAAGCTG CTACTGCCTC TCTCTCTTCT
    ATTATGATGG GTCTGGCCAA GCACTGGGCA ACAGTGATTG ATATGTTGTG CACATGTGTG
    AGTTTGTCTA CCACATGCCC TACCTTATCT ACTGCCAGCC TGCAGTTCCT TTCTATTCTC
    TTGACTGAAG AAGCAAAAAG GCATCTCCAG GATAAGAATA AAACAAATTC ATGCCACACT
    CCATCAATGG CTTCACTTCT CGATAAAACT CAAGAAAATC AGAAATCTCT AGAACGACTT
    AATGAAGTAA TTCTTCAGTG CTATGAAGGA AAATCCTCCA AAGATGTCCT GAAAAGAGTT
    GCTGCAAATA CACTGATGTC ACTTCTGGCT GTCAGTAGAA GAGCACAGAG ACAGGCTTTG
    AAAGCTGATC TAATAGACAG CTGCATGGAG CAGATGAAGC ACATTAATGC CCAGCTGAAC
    CTAGATTCTC TTAGGCCTGG GAAAGCAACC CTAAAGAAAC AGGAGGATGG TTTTATTAAA
    GAGTTAAGCA TTGCCATGCA GCTTCTAAGA AACTGTCTTT ATCAAAATGA AGAGTGTAAA
    GAAGCGGCTC TTGAATCTCA TCTTGTTCCT GTCTTGCACT CTCTCTGGCC TTGGCTTTTG
    ATGGATGATT CATTGATGCA AATTGCTCTG CAGCTACTTT GTGTCTATAC TGCAAATTTT
    CCAAATGGTT GCAGTTCTCT TTGTTGGTCA AGTTACGGAC AATATCCAGC TCAAGCTGCG
    TATAGAGGAG CCCCCAGCAC CTCTCTGATG GGGTGTATCC TGAAGTTGGC TTCCCAGATG
    CCTCTTGAGA ACACTGCAGT TCAGCAGATG GTTTTTATGT TTCTTTCAAA CCTGGCCTTA
    TCTCATGACT GTAAAGGAGT AATTCAGAAA AGTAACTTCT TACAGAACTT TCTATCTCTA
    ACATTGCCAA AAGGAGGAAA TAAACATCTC AGTAATCTGA CCATTCTTTG GTTGAAGTTA
    CTCCTGAATA TATCATTTGG AGAAGACGGG CAACAAATGA TCCTGAGGCT GGATGGCTGT
    CTAGACTTAC TTACAGAGAT GAGCAAATAT AAGCATAAGA GCAGCCCTTA TATACCTCTT
    CTTATCTTTC ATAATATTTG CTTCAGTCCT GCCAATAAGC CCAAGATCCT GGCTAATGAA
    AAAGTCATTA GTGTGCTAGC TGCCTGTCTG GAAAGCGAGA ATCAAAACGT TCAGAGGATT
    GGAGCAGCTG CACTTTGGGC TCTGATTCAC AATTATCAAA AGGCAAAAAT GACTTTGAAA
    AACCCATCCA TAAAAAGAAG AGTGGATGAA GCATATTCCT TAGCAAAGAA AACTTTCTCA
    AACTCAGAAG AAACCCCTCT AAATGCCTAT TACTTGAAAT GTCTTGAAAA TCTCGTTCAG
    CTTCTTAACT CTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00713
    Clone ID Related Accession (Same CDS sequence) XM_005615328.2 , XM_005615328.3
    Accession Version XM_005615328.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rotatin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005615328.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTTTA
    GATGACTCAT TCAAGTTTTG GAGAGCTCCA TCTAGGATGT CCAATCAAGA TCAAGATCCA
    AGTTCTCTCT CCACCTTGGA AACAATGGTG ACACCTTCAT CGGGGAGTAC TGAATTCCCA
    CCCTTTGGGC CCTCATCGGT GTCTCTCCCT GAGGCTTCAC ATGACCAGTT TGCGGCTCAA
    GTAATAGGTC CACATGAAAC TGCATCAGCA CAGCTTCCTC GTGACACATC CCTTCCTGCC
    CCTCTGCCCA AGCAGTGTGT GGTCACCCCA CCTCTCCTTT CAGCAGTGTG CAGCCTTCTG
    GACGGCCTCT TGGTAATTGC TCCAGGAGAC ACAGCACGTG CTTTTCGGCA GGCCCGTCTC
    ATAGAGCTTC TCTGTAGTAT TATGGATGCC ACCCTCCTAG AGAGCTGTGT GCAGGAGCTG
    AAGGGTCCAC TGCCTTTGTC ATCTCCAGTT GAACACACTC AGGCTCAGGT GTCCTTTCTC
    CTAGAATACC TGTCCTCTTT GTCTAGGCTT CTGCAGTCAT GTTTACTGGT AGATCCTGAC
    CTTGTGATTG AGGATGAGCT TCTGAAACCT CTGGTCACAA ACATCATTGG GGTCCTCACC
    ATATGCACCA AAGATGTGTT AGATGTAGAA TTGATATCTG CTTTTTATCA TACATGGACA
    CATTTATTTA ACCTTTTGGC TACGCTCCTG AGGAAAGCTG CTACTGCCTC TCTCTCTTCT
    ATTATGATGG GTCTGGCCAA GCACTGGGCA ACAGTGATTG ATATGTTGTG CACATGTGTG
    AGTTTGTCTA CCACATGCCC TACCTTATCT ACTGCCAGCC TGCAGTTCCT TTCTATTCTC
    TTGACTGAAG AAGCAAAAAG GCATCTCCAG GATAAGAATA AAACAAATTC ATGCCACACT
    CCATCAATGG CTTCACTTCT CGATAAAACT CAAGAAAATC AGAAATCTCT AGAACGACTT
    AATGAAGTAA TTCTTCAGTG CTATGAAGGA AAATCCTCCA AAGATGTCCT GAAAAGAGTT
    GCTGCAAATA CACTGATGTC ACTTCTGGCT GTCAGTAGAA GAGCACAGAG ACAGGCTTTG
    AAAGCTGATC TAATAGACAG CTGCATGGAG CAGATGAAGC ACATTAATGC CCAGCTGAAC
    CTAGATTCTC TTAGGCCTGG GAAAGCAACC CTAAAGAAAC AGGAGGATGG TTTTATTAAA
    GAGTTAAGCA TTGCCATGCA GCTTCTAAGA AACTGTCTTT ATCAAAATGA AGAGTGTAAA
    GAAGCGGCTC TTGAATCTCA TCTTGTTCCT GTCTTGCACT CTCTCTGGCC TTGGCTTTTG
    ATGGATGATT CATTGATGCA AATTGCTCTG CAGCTACTTT GTGTCTATAC TGCAAATTTT
    CCAAATGGTT GCAGTTCTCT TTGTTGGTCA AGTTACGGAC AATATCCAGC TCAAGCTGCG
    TATAGAGGAG CCCCCAGCAC CTCTCTGATG GGGTGTATCC TGAAGTTGGC TTCCCAGATG
    CCTCTTGAGA ACACTGCAGT TCAGCAGATG GTTTTTATGT TTCTTTCAAA CCTGGCCTTA
    TCTCATGACT GTAAAGGAGT AATTCAGAAA AGTAACTTCT TACAGAACTT TCTATCTCTA
    ACATTGCCAA AAGGAGGAAA TAAACATCTC AGTAATCTGA CCATTCTTTG GTTGAAGTTA
    CTCCTGAATA TATCATTTGG AGAAGACGGG CAACAAATGA TCCTGAGGCT GGATGGCTGT
    CTAGACTTAC TTACAGAGAT GAGCAAATAT AAGCATAAGA GCAGCCCTTA TATACCTCTT
    CTTATCTTTC ATAATATTTG CTTCAGTCCT GCCAATAAGC CCAAGATCCT GGCTAATGAA
    AAAGTCATTA GTGTGCTAGC TGCCTGTCTG GAAAGCGAGA ATCAAAACGT TCAGAGGATT
    GGAGCAGCTG CACTTTGGGC TCTGATTCAC AATTATCAAA AGGCAAAAAT GACTTTGAAA
    AACCCATCCA TAAAAAGAAG AGTGGATGAA GCATATTCCT TAGCAAAGAA AACTTTCTCA
    AACTCAGAAG AAACCCCTCT AAATGCCTAT TACTTGAAAT GTCTTGAAAA TCTCGTTCAG
    CTTCTTAACT CTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615385.1
    CDS276..6953
    Translation

    Target ORF information:

    RefSeq Version XM_005615328.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615328.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTCTGT TAATCCTTCC AATAAACCTT CTTCACCATC AGTCTTCAGT
    TTGCCTGTTT CAGTTTTTAG AAGGTATCAT CTACCAGTGC GTGTAGTTGG CCACCATGCT
    GTGAGTCCTT ACTCGATAGT TTTGCCATTA TCCACTGATT GTTTGGCCTT GAAACCAGTC
    TCAGACATGC TGAGAATAGC TTGGAACTTG TCCTGGTATC ATGGGAGTGA GAATCTGTTG
    AAGCAAATGA ATTCCGAATC TAAAATCCAA GAATTTTCAG ATACATTGCA GTTAACCACA
    GAGGACACCC TCACTCTGAA GATGACACAT ACAGCTAGTG GACTGAAGGA TTGTCTCCAT
    TCCATTGTCG AGGCTGCAGC CCACAGGGAA GTGAGGGCTG CTGTCACAAG AATGAGTTTT
    TATCTTCTGA ATGACAGACT GTCTTTAAAG GGTGGCTCTG GCCCATTTGG GGTTACCTTG
    AAGTCCTTGT CGTGGCATAC CACACTAAAC AGGTTTTTAC AGGTACTTCC TGCTTGCACT
    GAAGATGAGA AACTGCTAAT AGATATCATA CATTTTTTAA ATAAGTTACT GAAGGAACAA
    AGAAAAAACT CATCAGTGGA ACTTCTAAAC TGGCTTCTAG AATTACTTCT TAGACATAGT
    CCAAACCCAC TGTTAGACCT GCTGGTTCTG ACGGAGTCAC AGGCACGAGA GGAAACAGAT
    GACATCCAGA CTGCTGTCAG GCAACAGCTC CAGAAAGAAC TGATTGCTCT TTTCAATACC
    TTGCTGCTTA GTTTCATGGC AGTTACTGAC AGAAAATGCT TGGAACTTTT TTATGTTTTC
    CAAACACAGC TGTCTCTGAA ACTGCTCCAG TGCCTGAGAG TCACAGATGC TCCTCACTTC
    TATGGCCTGC CATCCCTTGA GAGGACCTTG CGAGGGATGG CTCATCTCAC AGCATCTCCA
    GGATGGAGCT CACATTCTCC TCTCACAAAG CCATTTGAAA TTTGTGTTAA GTACTTGTCA
    GGTCTACTTG AAGTCATTAC TTCTTTTTAT GTGGAGCGAG GAGGAAATGC TATGTCCTTT
    ATGGGAAAAG GGGTCACTAA GAACACAGTT CTTTGCTTGC TTCATTTATC CCACGAGATG
    ATGGCCCAGG CCCAAAGTTC GGAGTGGATG TCACTTTGGT TTTTGCCTTT GGGCAGTCAC
    AGTGAAGGAC ATGCTACCCC ACAAGGATTG GCGTGGCTCA TTCCATTATG GGTTGACCGA
    GACCCAGAGG TGAGGTTCAC TTCATTGGGA TTAGGATCAG CACTGACCAC CCTTGAAACT
    GGCTGTGTCG CGTTGGCAAA TAGTTGTCAA AACATTTCTG GTGGGCTCTG GGGGACAGTG
    GTGAACATTC TGCTGGACCA GTCGGAATGT AGTATGGTGC GCCGGGAGGC TGCATTTATT
    CTTCAGAATC TTCTTGTAAT TCCAATGCCT TCAGTAATTA TAAAGGATTA TACTTGGCAG
    GGTCCCTGTG TCCACGATGA GGATTCTGGC CTGTCGCTCA TAGGAAAACC TGCCCTTCAG
    GCTCTTCTAT ATCATTGCCA TTTCTATGAA CATTTGAATC AGATGATAAA ACACTGTTAC
    CTGGGACGAT ATACATTTGA TTTGAATTTT TCTGCTCTGG ATGGAACTTC AGAAGGTTTA
    GATGACTCAT TCAAGTTTTG GAGAGCTCCA TCTAGGATGT CCAATCAAGA TCAAGATCCA
    AGTTCTCTCT CCACCTTGGA AACAATGGTG ACACCTTCAT CGGGGAGTAC TGAATTCCCA
    CCCTTTGGGC CCTCATCGGT GTCTCTCCCT GAGGCTTCAC ATGACCAGTT TGCGGCTCAA
    GTAATAGGTC CACATGAAAC TGCATCAGCA CAGCTTCCTC GTGACACATC CCTTCCTGCC
    CCTCTGCCCA AGCAGTGTGT GGTCACCCCA CCTCTCCTTT CAGCAGTGTG CAGCCTTCTG
    GACGGCCTCT TGGTAATTGC TCCAGGAGAC ACAGCACGTG CTTTTCGGCA GGCCCGTCTC
    ATAGAGCTTC TCTGTAGTAT TATGGATGCC ACCCTCCTAG AGAGCTGTGT GCAGGAGCTG
    AAGGGTCCAC TGCCTTTGTC ATCTCCAGTT GAACACACTC AGGCTCAGGT GTCCTTTCTC
    CTAGAATACC TGTCCTCTTT GTCTAGGCTT CTGCAGTCAT GTTTACTGGT AGATCCTGAC
    CTTGTGATTG AGGATGAGCT TCTGAAACCT CTGGTCACAA ACATCATTGG GGTCCTCACC
    ATATGCACCA AAGATGTGTT AGATGTAGAA TTGATATCTG CTTTTTATCA TACATGGACA
    CATTTATTTA ACCTTTTGGC TACGCTCCTG AGGAAAGCTG CTACTGCCTC TCTCTCTTCT
    ATTATGATGG GTCTGGCCAA GCACTGGGCA ACAGTGATTG ATATGTTGTG CACATGTGTG
    AGTTTGTCTA CCACATGCCC TACCTTATCT ACTGCCAGCC TGCAGTTCCT TTCTATTCTC
    TTGACTGAAG AAGCAAAAAG GCATCTCCAG GATAAGAATA AAACAAATTC ATGCCACACT
    CCATCAATGG CTTCACTTCT CGATAAAACT CAAGAAAATC AGAAATCTCT AGAACGACTT
    AATGAAGTAA TTCTTCAGTG CTATGAAGGA AAATCCTCCA AAGATGTCCT GAAAAGAGTT
    GCTGCAAATA CACTGATGTC ACTTCTGGCT GTCAGTAGAA GAGCACAGAG ACAGGCTTTG
    AAAGCTGATC TAATAGACAG CTGCATGGAG CAGATGAAGC ACATTAATGC CCAGCTGAAC
    CTAGATTCTC TTAGGCCTGG GAAAGCAACC CTAAAGAAAC AGGAGGATGG TTTTATTAAA
    GAGTTAAGCA TTGCCATGCA GCTTCTAAGA AACTGTCTTT ATCAAAATGA AGAGTGTAAA
    GAAGCGGCTC TTGAATCTCA TCTTGTTCCT GTCTTGCACT CTCTCTGGCC TTGGCTTTTG
    ATGGATGATT CATTGATGCA AATTGCTCTG CAGCTACTTT GTGTCTATAC TGCAAATTTT
    CCAAATGGTT GCAGTTCTCT TTGTTGGTCA AGTTACGGAC AATATCCAGC TCAAGCTGCG
    TATAGAGGAG CCCCCAGCAC CTCTCTGATG GGGTGTATCC TGAAGTTGGC TTCCCAGATG
    CCTCTTGAGA ACACTGCAGT TCAGCAGATG GTTTTTATGT TTCTTTCAAA CCTGGCCTTA
    TCTCATGACT GTAAAGGAGT AATTCAGAAA AGTAACTTCT TACAGAACTT TCTATCTCTA
    ACATTGCCAA AAGGAGGAAA TAAACATCTC AGTAATCTGA CCATTCTTTG GTTGAAGTTA
    CTCCTGAATA TATCATTTGG AGAAGACGGG CAACAAATGA TCCTGAGGCT GGATGGCTGT
    CTAGACTTAC TTACAGAGAT GAGCAAATAT AAGCATAAGA GCAGCCCTTA TATACCTCTT
    CTTATCTTTC ATAATATTTG CTTCAGTCCT GCCAATAAGC CCAAGATCCT GGCTAATGAA
    AAAGTCATTA GTGTGCTAGC TGCCTGTCTG GAAAGCGAGA ATCAAAACGT TCAGAGGATT
    GGAGCAGCTG CACTTTGGGC TCTGATTCAC AATTATCAAA AGGCAAAAAT GACTTTGAAA
    AACCCATCCA TAAAAAGAAG AGTGGATGAA GCATATTCCT TAGCAAAGAA AACTTTCTCA
    AACTCAGAAG AAACCCCTCT AAATGCCTAT TACTTGAAAT GTCTTGAAAA TCTCGTTCAG
    CTTCTTAACT CTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe00715
    Clone ID Related Accession (Same CDS sequence) XM_005615329.2
    Accession Version XM_005615329.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6609bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rotatin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726046.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005615329.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTATCA TCTACCAGTG CGTGTAGTTG GCCACCATGC TGTGAGTCCT
    TACTCGATAG TTTTGCCATT ATCCACTGAT TGTTTGGCCT TGAAACCAGT CTCAGACATG
    CTGAGAATAG CTTGGAACTT GTCCTGGTAT CATGGGAGTG AGAATCTGTT GAAGCAAATG
    AATTCCGAAT CTAAAATCCA AGAATTTTCA GATACATTGC AGTTAACCAC AGAGGACACC
    CTCACTCTGA AGATGACACA TACAGCTAGT GGACTGAAGG ATTGTCTCCA TTCCATTGTC
    GAGGCTGCAG CCCACAGGGA AGTGAGGGCT GCTGTCACAA GAATGAGTTT TTATCTTCTG
    AATGACAGAC TGTCTTTAAA GGGTGGCTCT GGCCCATTTG GGGTTACCTT GAAGTCCTTG
    TCGTGGCATA CCACACTAAA CAGGTTTTTA CAGGTACTTC CTGCTTGCAC TGAAGATGAG
    AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAGTTAC TGAAGGAACA AAGAAAAAAC
    TCATCAGTGG AACTTCTAAA CTGGCTTCTA GAATTACTTC TTAGACATAG TCCAAACCCA
    CTGTTAGACC TGCTGGTTCT GACGGAGTCA CAGGCACGAG AGGAAACAGA TGACATCCAG
    ACTGCTGTCA GGCAACAGCT CCAGAAAGAA CTGATTGCTC TTTTCAATAC CTTGCTGCTT
    AGTTTCATGG CAGTTACTGA CAGAAAATGC TTGGAACTTT TTTATGTTTT CCAAACACAG
    CTGTCTCTGA AACTGCTCCA GTGCCTGAGA GTCACAGATG CTCCTCACTT CTATGGCCTG
    CCATCCCTTG AGAGGACCTT GCGAGGGATG GCTCATCTCA CAGCATCTCC AGGATGGAGC
    TCACATTCTC CTCTCACAAA GCCATTTGAA ATTTGTGTTA AGTACTTGTC AGGTCTACTT
    GAAGTCATTA CTTCTTTTTA TGTGGAGCGA GGAGGAAATG CTATGTCCTT TATGGGAAAA
    GGGGTCACTA AGAACACAGT TCTTTGCTTG CTTCATTTAT CCCACGAGAT GATGGCCCAG
    GCCCAAAGTT CGGAGTGGAT GTCACTTTGG TTTTTGCCTT TGGGCAGTCA CAGTGAAGGA
    CATGCTACCC CACAAGGATT GGCGTGGCTC ATTCCATTAT GGGTTGACCG AGACCCAGAG
    GTGAGGTTCA CTTCATTGGG ATTAGGATCA GCACTGACCA CCCTTGAAAC TGGCTGTGTC
    GCGTTGGCAA ATAGTTGTCA AAACATTTCT GGTGGGCTCT GGGGGACAGT GGTGAACATT
    CTGCTGGACC AGTCGGAATG TAGTATGGTG CGCCGGGAGG CTGCATTTAT TCTTCAGAAT
    CTTCTTGTAA TTCCAATGCC TTCAGTAATT ATAAAGGATT ATACTTGGCA GGGTCCCTGT
    GTCCACGATG AGGATTCTGG CCTGTCGCTC ATAGGAAAAC CTGCCCTTCA GGCTCTTCTA
    TATCATTGCC ATTTCTATGA ACATTTGAAT CAGATGATAA AACACTGTTA CCTGGGACGA
    TATACATTTG ATTTGAATTT TTCTGCTCTG GATGGAACTT CAGAAGGTTT AGATGACTCA
    TTCAAGTTTT GGAGAGCTCC ATCTAGGATG TCCAATCAAG ATCAAGATCC AAGTTCTCTC
    TCCACCTTGG AAACAATGGT GACACCTTCA TCGGGGAGTA CTGAATTCCC ACCCTTTGGG
    CCCTCATCGG TGTCTCTCCC TGAGGCTTCA CATGACCAGT TTGCGGCTCA AGTAATAGGT
    CCACATGAAA CTGCATCAGC ACAGCTTCCT CGTGACACAT CCCTTCCTGC CCCTCTGCCC
    AAGCAGTGTG TGGTCACCCC ACCTCTCCTT TCAGCAGTGT GCAGCCTTCT GGACGGCCTC
    TTGGTAATTG CTCCAGGAGA CACAGCACGT GCTTTTCGGC AGGCCCGTCT CATAGAGCTT
    CTCTGTAGTA TTATGGATGC CACCCTCCTA GAGAGCTGTG TGCAGGAGCT GAAGGGTCCA
    CTGCCTTTGT CATCTCCAGT TGAACACACT CAGGCTCAGG TGTCCTTTCT CCTAGAATAC
    CTGTCCTCTT TGTCTAGGCT TCTGCAGTCA TGTTTACTGG TAGATCCTGA CCTTGTGATT
    GAGGATGAGC TTCTGAAACC TCTGGTCACA AACATCATTG GGGTCCTCAC CATATGCACC
    AAAGATGTGT TAGATGTAGA ATTGATATCT GCTTTTTATC ATACATGGAC ACATTTATTT
    AACCTTTTGG CTACGCTCCT GAGGAAAGCT GCTACTGCCT CTCTCTCTTC TATTATGATG
    GGTCTGGCCA AGCACTGGGC AACAGTGATT GATATGTTGT GCACATGTGT GAGTTTGTCT
    ACCACATGCC CTACCTTATC TACTGCCAGC CTGCAGTTCC TTTCTATTCT CTTGACTGAA
    GAAGCAAAAA GGCATCTCCA GGATAAGAAT AAAACAAATT CATGCCACAC TCCATCAATG
    GCTTCACTTC TCGATAAAAC TCAAGAAAAT CAGAAATCTC TAGAACGACT TAATGAAGTA
    ATTCTTCAGT GCTATGAAGG AAAATCCTCC AAAGATGTCC TGAAAAGAGT TGCTGCAAAT
    ACACTGATGT CACTTCTGGC TGTCAGTAGA AGAGCACAGA GACAGGCTTT GAAAGCTGAT
    CTAATAGACA GCTGCATGGA GCAGATGAAG CACATTAATG CCCAGCTGAA CCTAGATTCT
    CTTAGGCCTG GGAAAGCAAC CCTAAAGAAA CAGGAGGATG GTTTTATTAA AGAGTTAAGC
    ATTGCCATGC AGCTTCTAAG AAACTGTCTT TATCAAAATG AAGAGTGTAA AGAAGCGGCT
    CTTGAATCTC ATCTTGTTCC TGTCTTGCAC TCTCTCTGGC CTTGGCTTTT GATGGATGAT
    TCATTGATGC AAATTGCTCT GCAGCTACTT TGTGTCTATA CTGCAAATTT TCCAAATGGT
    TGCAGTTCTC TTTGTTGGTC AAGTTACGGA CAATATCCAG CTCAAGCTGC GTATAGAGGA
    GCCCCCAGCA CCTCTCTGAT GGGGTGTATC CTGAAGTTGG CTTCCCAGAT GCCTCTTGAG
    AACACTGCAG TTCAGCAGAT GGTTTTTATG TTTCTTTCAA ACCTGGCCTT ATCTCATGAC
    TGTAAAGGAG TAATTCAGAA AAGTAACTTC TTACAGAACT TTCTATCTCT AACATTGCCA
    AAAGGAGGAA ATAAACATCT CAGTAATCTG ACCATTCTTT GGTTGAAGTT ACTCCTGAAT
    ATATCATTTG GAGAAGACGG GCAACAAATG ATCCTGAGGC TGGATGGCTG TCTAGACTTA
    CTTACAGAGA TGAGCAAATA TAAGCATAAG AGCAGCCCTT ATATACCTCT TCTTATCTTT
    CATAATATTT GCTTCAGTCC TGCCAATAAG CCCAAGATCC TGGCTAATGA AAAAGTCATT
    AGTGTGCTAG CTGCCTGTCT GGAAAGCGAG AATCAAAACG TTCAGAGGAT TGGAGCAGCT
    GCACTTTGGG CTCTGATTCA CAATTATCAA AAGGCAAAAA TGACTTTGAA AAACCCATCC
    ATAAAAAGAA GAGTGGATGA AGCATATTCC TTAGCAAAGA AAACTTTCTC AAACTCAGAA
    GAAACCCCTC TAAATGCCTA TTACTTGAAA TGTCTTGAAA ATCTCGTTCA GCTTCTTAAC
    TCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005615386.1
    CDS344..6952
    Translation

    Target ORF information:

    RefSeq Version XM_005615329.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris rotatin (RTTN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005615329.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGGAGCGGG ACGACGCGGT CTTGACGGGG CTCATCAGGA AACTCGGGCA TCAGCTGGCG 
    GAGATCCGCG AGCGCGCGCT GCGGAGCATC CTGTGCAAGC TGGAGCTCGG CCTGCTCGGC
    TGCGAGGACG TGGTGCAGGA CCAGCCGCTC TTCCTGCGTC TGCTGGCCTG GTTCAACTTC
    CCGACCGTTC CCATGAAGGA GGAGGTTCTC AGCCTGTTGA ACAGATTGGT GAAGTATCCC
    CCAGCAGTCC AGCACTTGAT TGATCTTGGT GCTGTCGAAT TCTTATCTAA ACTTCGTTGT
    AATGTGGAGC CAAATCTACA GGCTGAAATT GATGGCATTC TGGATGGACT TTTTATTCTT
    CCTTCAGTAG ATGCTGCATC TTACCAAACC AAACAAACTG AAATGCCACA ACAACCTGAA
    ATCTTAATAG GATATTTTCC TAAAGACAAA AGTAATCTCC AGCAGATGGA AGTGTCTCCA
    CGACCAATGG TGAATCAGAC TGTGAAGTGT TTGAAGTTTT CTACGTTTCC TTGGCTACCC
    CTGACTACAA CAGATAGACA TGTTCTCTCC TCTAATGAAA GCTCTTTAAG AAGTAGTAAC
    CATACTTTAA TTTGGAACAC CTGTGAGCTA CTGAAGGACG TTATCATGCA AGACTTTCCT
    GCTGAAATTT TCCTTCAAAG GCCAAAAATT GTTCAGAGCC TTTTATCTCT GTTGAAACTG
    GCCTTTGGTG GAGATGGAAG ACATCGCCTG GCATTACAGT CAGTGTCCTG CTTGCAGCAG
    CTGTGCACAT ACTTAAGAAA CAGACTTAAC TTTCACCGAG ATCCAGGTTT TTTCTCCAGT
    AAAGAAGATA CAGTTTCCCA GAATTCTTCT TTGTCATACT GTCATGAAGT TAGAGTTATT
    CATCATTCTC AGAATCCTTC TCTGGGAAGC AGTAGCCCCC GGCCTTCTGT GGTTGGACGC
    ACAGGCCAGC GACCCAGAGG AGATGGCCAG GATTGGGATG CTGTGTCTTC CAGTGGAAGT
    AGTAGTCATG CTCCTGTGAA CTCTAGGATC TCTGTGCATT CGCCTTTGGA TGTGGTACAC
    ATAGATCTGC CTGAGCTGGA GGCCGAAGAC ACTCTGGAAC TACAATTCCA GCAGCTCAGT
    CTGCCCCAGT TCTGTGTCTC CGTTCTGCAA TCAGCAGTTC CTCTCTTAAG AACAGGTAGT
    AGACAAATGA TAATAAGAGT TCTGGAATTG CTTGCAGAGG ATATGATACT TATTGGTGAA
    GCAATTTCAG CCGATATCTG GGATGACAAC AGCCTTTTTG CTGTAGATAT GAAAGAAAAA
    CTGCTTATGG TGTTGAGGTC CCTGGGAGAT ACCATATGCT ACCATAAAAG TGGAGTGAGC
    CAGGACCAGC CAGAGTTAGT GCTGGTGCAC CACCGAATGG CCTTTGTCAG CGTTTCACTG
    TTTGCAGTCC GAGTCCTGCA GATGCTTCTC CCTGTTGAAA AGGCAAGCCA CCTTTTACCG
    GAGCCTATGT CAGCAGCATT ATTTCTAGTT TCTTTGGACA TGCCTGTTTC TTTGGAATAT
    CCAAATATTC ATGAAGCTGT TGTGGCATAT TTGGAACAGT TGAATTCTGA AAACTACAGT
    ATTTATAAAC AAACTGCAGA GGCTGTTTAT TCAATTGAAT GTACCTGTAA CTTCCTGTCT
    GATGTTGGGA AGGAGGGCGA GAAGAATCTC TTGGAATTAG TAGAGCTGGC AGACCAAGCC
    TTGAGAAGCT TTTCTTATCA TCAGCATTTC CCCCTAATAA AGGAAATGAT CTGCATGTGT
    TCAAAGATCT GCAAATCTGC GCAGGCCAGT CCATTACTTC AAGGAGAAAG TCAGAAGGTG
    TTTCTGCGCA TGTTATCTCA CCCATTACTG CAAGTGAAAA TTGAAACTTA TAACTGCTGT
    CTGGAAATTA CTAAGGAATG TTTAGGTGTC TATAACGTCA CTAAACCTGT GTCTTCACTT
    TGTAACGGAA TTCATTTTCT ACTTCACCCG AAAGTTCTGT ATGAAATCTC TGCATTTGGC
    ATTCAAGAGC CCAAAAATGA GGTGAATGCT GCTGCCAAGG CCATTTTGCT GTATCTGCTG
    CAGGGACGAT TGATGATGAC AGCAGTGACC TGGAACAAGT TTATTGAGTC TCTCTGTCCT
    GTCATTCCTG TGCTGCAGGG CTATGCTGAC ACAGAAGATC CTTTGGGTAA CTCCATTCTC
    CTTCTGACGA AAGGGAATCC TGAGGCGGGC GAAGGGGCTC TCCCCTGTAC CACCCGACTG
    AAGTCCATGC TGCGGCTCCT GTTTGTCAGG AAGCCATTGG TCCGTTCACT AGCATTAAAG
    CTTTTAGCAT GTCATCTCAC AAGGGAAGAA GGGGCTGACA CAAAGCGTCC TTTAATAGAT
    GCCAGAATTC TCTCCAGAGT TACTAATTTA TTTATTGTGA AAAAACCTAT TGAACTCACG
    TTGGATGACA GAAGAGAACT GATTATTAGG TTGGAGACTG TTGAAAAGGT TTATAAAATC
    TTTACCTCAG ATGATATTGA TCTTGTTTTG AGAAAGTCAG CCGCAGAACA GTTAGCTGTG
    ATTATGCAAG ATATTAAAAT GCATGCTGTG GTGAAAAAGT TACACTTAGT TGACACGATA
    ATTGAGTATC TAAATGAATG TGTAGGTCAG GATGGTGAGC TCATAGAATG TTTGATTCTG
    CCAAGCCTCA CACTCTTCAG GAAAGTTCTT TGTGCGGACC CGGTCATGCG CTTGGCCCTC
    TCACAGCAGG CTTCTCTTTT GACCCTGTTA TTCAGAGTTT CCTTAATATT TCATGAAAAT
    TGTGCTGTCG TGACTGAAGT CGGAGCATTA TTTTGTCTTC TATTATTTGA TGAAGTATCA
    AGAATGGATA TGTGGTATCA TCTACCAGTG CGTGTAGTTG GCCACCATGC TGTGAGTCCT
    TACTCGATAG TTTTGCCATT ATCCACTGAT TGTTTGGCCT TGAAACCAGT CTCAGACATG
    CTGAGAATAG CTTGGAACTT GTCCTGGTAT CATGGGAGTG AGAATCTGTT GAAGCAAATG
    AATTCCGAAT CTAAAATCCA AGAATTTTCA GATACATTGC AGTTAACCAC AGAGGACACC
    CTCACTCTGA AGATGACACA TACAGCTAGT GGACTGAAGG ATTGTCTCCA TTCCATTGTC
    GAGGCTGCAG CCCACAGGGA AGTGAGGGCT GCTGTCACAA GAATGAGTTT TTATCTTCTG
    AATGACAGAC TGTCTTTAAA GGGTGGCTCT GGCCCATTTG GGGTTACCTT GAAGTCCTTG
    TCGTGGCATA CCACACTAAA CAGGTTTTTA CAGGTACTTC CTGCTTGCAC TGAAGATGAG
    AAACTGCTAA TAGATATCAT ACATTTTTTA AATAAGTTAC TGAAGGAACA AAGAAAAAAC
    TCATCAGTGG AACTTCTAAA CTGGCTTCTA GAATTACTTC TTAGACATAG TCCAAACCCA
    CTGTTAGACC TGCTGGTTCT GACGGAGTCA CAGGCACGAG AGGAAACAGA TGACATCCAG
    ACTGCTGTCA GGCAACAGCT CCAGAAAGAA CTGATTGCTC TTTTCAATAC CTTGCTGCTT
    AGTTTCATGG CAGTTACTGA CAGAAAATGC TTGGAACTTT TTTATGTTTT CCAAACACAG
    CTGTCTCTGA AACTGCTCCA GTGCCTGAGA GTCACAGATG CTCCTCACTT CTATGGCCTG
    CCATCCCTTG AGAGGACCTT GCGAGGGATG GCTCATCTCA CAGCATCTCC AGGATGGAGC
    TCACATTCTC CTCTCACAAA GCCATTTGAA ATTTGTGTTA AGTACTTGTC AGGTCTACTT
    GAAGTCATTA CTTCTTTTTA TGTGGAGCGA GGAGGAAATG CTATGTCCTT TATGGGAAAA
    GGGGTCACTA AGAACACAGT TCTTTGCTTG CTTCATTTAT CCCACGAGAT GATGGCCCAG
    GCCCAAAGTT CGGAGTGGAT GTCACTTTGG TTTTTGCCTT TGGGCAGTCA CAGTGAAGGA
    CATGCTACCC CACAAGGATT GGCGTGGCTC ATTCCATTAT GGGTTGACCG AGACCCAGAG
    GTGAGGTTCA CTTCATTGGG ATTAGGATCA GCACTGACCA CCCTTGAAAC TGGCTGTGTC
    GCGTTGGCAA ATAGTTGTCA AAACATTTCT GGTGGGCTCT GGGGGACAGT GGTGAACATT
    CTGCTGGACC AGTCGGAATG TAGTATGGTG CGCCGGGAGG CTGCATTTAT TCTTCAGAAT
    CTTCTTGTAA TTCCAATGCC TTCAGTAATT ATAAAGGATT ATACTTGGCA GGGTCCCTGT
    GTCCACGATG AGGATTCTGG CCTGTCGCTC ATAGGAAAAC CTGCCCTTCA GGCTCTTCTA
    TATCATTGCC ATTTCTATGA ACATTTGAAT CAGATGATAA AACACTGTTA CCTGGGACGA
    TATACATTTG ATTTGAATTT TTCTGCTCTG GATGGAACTT CAGAAGGTTT AGATGACTCA
    TTCAAGTTTT GGAGAGCTCC ATCTAGGATG TCCAATCAAG ATCAAGATCC AAGTTCTCTC
    TCCACCTTGG AAACAATGGT GACACCTTCA TCGGGGAGTA CTGAATTCCC ACCCTTTGGG
    CCCTCATCGG TGTCTCTCCC TGAGGCTTCA CATGACCAGT TTGCGGCTCA AGTAATAGGT
    CCACATGAAA CTGCATCAGC ACAGCTTCCT CGTGACACAT CCCTTCCTGC CCCTCTGCCC
    AAGCAGTGTG TGGTCACCCC ACCTCTCCTT TCAGCAGTGT GCAGCCTTCT GGACGGCCTC
    TTGGTAATTG CTCCAGGAGA CACAGCACGT GCTTTTCGGC AGGCCCGTCT CATAGAGCTT
    CTCTGTAGTA TTATGGATGC CACCCTCCTA GAGAGCTGTG TGCAGGAGCT GAAGGGTCCA
    CTGCCTTTGT CATCTCCAGT TGAACACACT CAGGCTCAGG TGTCCTTTCT CCTAGAATAC
    CTGTCCTCTT TGTCTAGGCT TCTGCAGTCA TGTTTACTGG TAGATCCTGA CCTTGTGATT
    GAGGATGAGC TTCTGAAACC TCTGGTCACA AACATCATTG GGGTCCTCAC CATATGCACC
    AAAGATGTGT TAGATGTAGA ATTGATATCT GCTTTTTATC ATACATGGAC ACATTTATTT
    AACCTTTTGG CTACGCTCCT GAGGAAAGCT GCTACTGCCT CTCTCTCTTC TATTATGATG
    GGTCTGGCCA AGCACTGGGC AACAGTGATT GATATGTTGT GCACATGTGT GAGTTTGTCT
    ACCACATGCC CTACCTTATC TACTGCCAGC CTGCAGTTCC TTTCTATTCT CTTGACTGAA
    GAAGCAAAAA GGCATCTCCA GGATAAGAAT AAAACAAATT CATGCCACAC TCCATCAATG
    GCTTCACTTC TCGATAAAAC TCAAGAAAAT CAGAAATCTC TAGAACGACT TAATGAAGTA
    ATTCTTCAGT GCTATGAAGG AAAATCCTCC AAAGATGTCC TGAAAAGAGT TGCTGCAAAT
    ACACTGATGT CACTTCTGGC TGTCAGTAGA AGAGCACAGA GACAGGCTTT GAAAGCTGAT
    CTAATAGACA GCTGCATGGA GCAGATGAAG CACATTAATG CCCAGCTGAA CCTAGATTCT
    CTTAGGCCTG GGAAAGCAAC CCTAAAGAAA CAGGAGGATG GTTTTATTAA AGAGTTAAGC
    ATTGCCATGC AGCTTCTAAG AAACTGTCTT TATCAAAATG AAGAGTGTAA AGAAGCGGCT
    CTTGAATCTC ATCTTGTTCC TGTCTTGCAC TCTCTCTGGC CTTGGCTTTT GATGGATGAT
    TCATTGATGC AAATTGCTCT GCAGCTACTT TGTGTCTATA CTGCAAATTT TCCAAATGGT
    TGCAGTTCTC TTTGTTGGTC AAGTTACGGA CAATATCCAG CTCAAGCTGC GTATAGAGGA
    GCCCCCAGCA CCTCTCTGAT GGGGTGTATC CTGAAGTTGG CTTCCCAGAT GCCTCTTGAG
    AACACTGCAG TTCAGCAGAT GGTTTTTATG TTTCTTTCAA ACCTGGCCTT ATCTCATGAC
    TGTAAAGGAG TAATTCAGAA AAGTAACTTC TTACAGAACT TTCTATCTCT AACATTGCCA
    AAAGGAGGAA ATAAACATCT CAGTAATCTG ACCATTCTTT GGTTGAAGTT ACTCCTGAAT
    ATATCATTTG GAGAAGACGG GCAACAAATG ATCCTGAGGC TGGATGGCTG TCTAGACTTA
    CTTACAGAGA TGAGCAAATA TAAGCATAAG AGCAGCCCTT ATATACCTCT TCTTATCTTT
    CATAATATTT GCTTCAGTCC TGCCAATAAG CCCAAGATCC TGGCTAATGA AAAAGTCATT
    AGTGTGCTAG CTGCCTGTCT GGAAAGCGAG AATCAAAACG TTCAGAGGAT TGGAGCAGCT
    GCACTTTGGG CTCTGATTCA CAATTATCAA AAGGCAAAAA TGACTTTGAA AAACCCATCC
    ATAAAAAGAA GAGTGGATGA AGCATATTCC TTAGCAAAGA AAACTTTCTC AAACTCAGAA
    GAAACCCCTC TAAATGCCTA TTACTTGAAA TGTCTTGAAA ATCTCGTTCA GCTTCTTAAC
    TCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.