BEST1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	BEST1
Entrez Gene ID	483791
Full Name	bestrophin 1
Synonyms	VMD2
General protein information	Preferred Names bestrophin 1 Names bestrophin-1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022405085.1	XP_022260793.1	bestrophin-1 isoform X2
XM_014120876.2	XP_013976351.1	bestrophin-1 isoform X1
XM_022405083.1	XP_022260791.1	bestrophin-1 isoform X1
XM_014120876.1	XP_013976351.1	bestrophin-1 isoform X1
NM_001097545.1	NP_001091014.1	bestrophin-1
XM_022405084.1	XP_022260792.1	bestrophin-1 isoform X1
XM_022405086.1	XP_022260794.1	bestrophin-1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	BEST1	NP_004174.1
Bos taurus (cattle)	BEST1	NP_001073714.1
Mus musculus (house mouse)	Best1	NP_036043.2
Danio rerio (zebrafish)	LOC560608	XP_689098.3
Pan troglodytes (chimpanzee)	BEST1	XP_001151529.1
	LOC718993	XP_001116591.1
Xenopus tropicalis (tropical clawed frog)	best1	NP_001155223.1
Canis lupus familiaris (dog)	BEST1	NP_001091014.1
Rattus norvegicus (Norway rat)	Best1	NP_001011940.1
Gallus gallus (chicken)	BEST1	XP_004941545.1

Gene Ontology
Bibliography

Related articles in PubMed

Modeling the structural consequences of BEST1 missense mutations.
Guziewicz KE, Aguirre GD, Zangerl B
Advances in experimental medicine and biology723611-8(2012)

Assessment of canine BEST1 variations identifies new mutations and establishes an independent bestrophinopathy model (cmr3).
Zangerl B, Wickstr?m K, Slavik J, Lindauer SJ, Ahonen S, Schelling C, Lohi H, Guziewicz KE, Aguirre GD
Molecular vision162791-804(2010 Dec)

Multifocal retinal dysplasia in the German Spitz (Klein and Mittel) is not caused by mutations in BEST1.
Aguirre-Hern?ndez J, Sargan DR
Animal genetics41(3)333-4(2010 Jun)

Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.
Guziewicz KE, Zangerl B, Lindauer SJ, Mullins RF, Sandmeyer LS, Grahn BH, Stone EM, Acland GM, Aguirre GD
Investigative ophthalmology & visual science48(5)1959-67(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bestrophinopathies are a group of inherited retinal disorders primarily caused by point mutations scattered throughout the BEST1 gene.
Title: Modeling the structural consequences of BEST1 missense mutations.

Seven novel coding variants were identified in exon 10 of cBEST1.
Title: Assessment of canine BEST1 variations identifies new mutations and establishes an independent bestrophinopathy model (cmr3).

The mutation underlying Multifocal retinal dysplasia in the German Spitz is not allelic with BEST1 mutations found in four other breeds.
Title: Multifocal retinal dysplasia in the German Spitz (Klein and Mittel) is not caused by mutations in BEST1.

Two spontaneous mutations in the canine VMD2 gene, which cause canine multifocal retinopathy, as the first naturally occurring animal model of BMD (Best macular dystrophy).
Title: Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.

The following BEST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe33954	NM_001097545.1 Latest version!	Canis lupus familiaris bestrophin 1 (BEST1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe33954	XM_022405085.1 Latest version!	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe294596	XM_022405086.1 Latest version!	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe41041	XM_022405084.1 Latest version!	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe41041	XM_022405083.1 Latest version!	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe41041	XM_014120876.2 Latest version!	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe41041	XM_014120876.1	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BEST1 ( NM_001097545.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001091014.1 Canis lupus familiaris bestrophin 1 (BEST1), mRNA.

Price & Availability↑

CloneID	OCe33954
Clone ID Related Accession (Same CDS sequence)	XM_022405085.1 , NM_001097545.1
Accession Version	NM_001097545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	bestrophin-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF110978.1. On May 24, 2007 this sequence version replaced XM_540912.2. ##Evidence-Data-START## Transcript exon combination :: EF110978.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091014.1
CDS	110..1852
Translation	MTVTYSSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLSYYTIRFIYRMALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	NM_001097545.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097545.1

ORF Insert Sequence:

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_022405085.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260793.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe33954
Clone ID Related Accession (Same CDS sequence)	XM_022405085.1 , NM_001097545.1
Accession Version	XM_022405085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bestrophin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260793.1
CDS	597..2339
Translation	MTVTYSSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLSYYTIRFIYRMALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_022405085.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405085.1

ORF Insert Sequence:

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGGTG GCAGAACAGC TCATCAACCC ATTTGGAGAG 
GATGATGATG ACTTTGAGAC CAACTGGATT GTTGACAGGA GTTTGCAGGT GTCCCTGTTG 
GCTGTGGACG AGATGCACCA GGACCTGCCC CCGATGGAGC GGGATATGTA CTGGAATGAA 
CCAGAACCAC AACCCCCCTA CACGGCTGCT TCTGCCCAGT ATCGTCGAGC CTCCTTTTTA 
GGCTCCACTT TCAACATCAA TCTGCATAAG GAAGACATGG AGGAGGAAGA GGAAGGCACT 
CACGCTGGCA TCATTGGCCG CTTTCTAGGG CTGCAGTCCC ACGACCACCA ACCCCCCAGG 
ACAAACTCAA AGACCAAACT ACTATGGCCC AAGAAAGAAG GCTTTCTCCA AGACGGCCAG 
CCCAAGAAAC TCGGGGGTGC CAGGCAGACC TCTAGGGACA AGGAAGACAG CAAGGCCTGG 
GAGATTAAGG AGGAGGGTGC TTTTGAGACT GCTGCATTTT ATGGGAGGTC AGGCTACCAC 
AGTGCCCCAC AGACGCCCCT CAGCCACACA CCTATGGTCT TCCCATCTGG ACAGTCAGCA 
CCCTCAAGCC TACGCAGAGT CTCAGGGATA GACGGCGCTG TCAAAGACCA AAGCCTACAG 
CCTGGGATAA AGACCAGTTC TGAAGCACTC CCAGAGAATG TTGGGTTCTC ATTAGAGAAC 
CCAGAATCGG GTCACGTGAA GAGGAAAACT GTCGAGTTTA ACCTGACAGA CATGTCGGGG 
ACCCCTGAAC ATCATCTCAG AGACTCAGAT TTGAGCCAGT CAACTAGCAA CTTACACACT 
ATACTCAAGG GCCACGGAGA TCCCTATTGG GCCTTGGAAA ACAGGGATGA AGCACATTCT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_022405086.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260794.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe294596
Clone ID Related Accession (Same CDS sequence)	XM_022405086.1
Accession Version	XM_022405086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bestrophin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTCA CGGAGGAACA GCAGGTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACTCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAA CCTGGTGTCG 
GGCTTGGTGG AGGGCAGGGA CGAGCAGGGC CGGCTGCTGC GCCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCG CCGCCGTCTA CAAGCGCTTC 
CCCAGCTCCC AGCACCTGGT GAAAGCAGGC TTTATGACCC CCGCGGAATG CAAGCACTTA 
GAAAAGCTGA GCTTGCCGCA CAACACGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGATTGG AGGTCGAATC CGGGACCCTG TCCTGCTCCA GAGCCTGCTG 
GACGAGATGA ACATCTTGCG TACTCAGTGT GGACACCTGT TTGCCTACGA CTGGATCAGT 
ATCCCGCTGG TGTACACGCA GGTGGTGACC GTGGCTGTAT ACAGCTTCTT CCTGGCTTGC 
CTGGTTGGGC GGCAGTTCCT GAACCCAGCC AAGGCCTACC CTGGCCACGA GCTGGACCTT 
GTTGTACCTG TCTTCACATT TCTGCAGTTC TTCTTCTATG CTGGCTGGCT GAAGTGGTGC 
TGGGAGGTGG CAGAACAGCT CATCAACCCA TTTGGAGAGG ATGATGATGA CTTTGAGACC 
AACTGGATTG TTGACAGGAG TTTGCAGGTG TCCCTGTTGG CTGTGGACGA GATGCACCAG 
GACCTGCCCC CGATGGAGCG GGATATGTAC TGGAATGAAC CAGAACCACA ACCCCCCTAC 
ACGGCTGCTT CTGCCCAGTA TCGTCGAGCC TCCTTTTTAG GCTCCACTTT CAACATCAAT 
CTGCATAAGG AAGACATGGA GGAGGAAGAG GAAGGCACTC ACGCTGGCAT CATTGGCCGC 
TTTCTAGGGC TGCAGTCCCA CGACCACCAA CCCCCCAGGA CAAACTCAAA GACCAAACTA 
CTATGGCCCA AGAAAGAAGG CTTTCTCCAA GACGGCCAGC CCAAGAAACT CGGGGGTGCC 
AGGCAGACCT CTAGGGACAA GGAAGACAGC AAGGCCTGGG AGATTAAGGA GGAGGGTGCT 
TTTGAGACTG CTGCATTTTA TGGGAGGTCA GGCTACCACA GTGCCCCACA GACGCCCCTC 
AGCCACACAC CTATGGTCTT CCCATCTGGA CAGTCAGCAC CCTCAAGCCT ACGCAGAGTC 
TCAGGGATAG ACGGCGCTGT CAAAGACCAA AGCCTACAGC CTGGGATAAA GACCAGTTCT 
GAAGCACTCC CAGAGAATGT TGGGTTCTCA TTAGAGAACC CAGAATCGGG TCACGTGAAG 
AGGAAAACTG TCGAGTTTAA CCTGACAGAC ATGTCGGGGA CCCCTGAACA TCATCTCAGA 
GACTCAGATT TGAGCCAGTC AACTAGCAAC TTACACACTA TACTCAAGGG CCACGGAGAT 
CCCTATTGGG CCTTGGAAAA CAGGGATGAA GCACATTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260794.1
CDS	136..1737
Translation	MALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKWCWEVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_022405086.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405086.1

ORF Insert Sequence:

ATGGCCCTCA CGGAGGAACA GCAGGTGATG TTTGAGAAAC TGACTCTGTA TTGCGACAGC 
TACATCCAGC TCATCCCCAT TTCCTTCGTG CTGGGCTTCT ACGTGACTCT GGTCGTGACC 
CGCTGGTGGA ACCAGTACGA GAACCTGCCG TGGCCCGACC GCCTCATGAA CCTGGTGTCG 
GGCTTGGTGG AGGGCAGGGA CGAGCAGGGC CGGCTGCTGC GCCGCACGCT CATCCGCTAC 
GCCAACCTGG GCAACGTGCT CATCCTGCGC AGCGTCAGCG CCGCCGTCTA CAAGCGCTTC 
CCCAGCTCCC AGCACCTGGT GAAAGCAGGC TTTATGACCC CCGCGGAATG CAAGCACTTA 
GAAAAGCTGA GCTTGCCGCA CAACACGTTC TGGGTGCCCT GGGTGTGGTT TGCCAACCTG 
TCAATGAAGG CGTGGATTGG AGGTCGAATC CGGGACCCTG TCCTGCTCCA GAGCCTGCTG 
GACGAGATGA ACATCTTGCG TACTCAGTGT GGACACCTGT TTGCCTACGA CTGGATCAGT 
ATCCCGCTGG TGTACACGCA GGTGGTGACC GTGGCTGTAT ACAGCTTCTT CCTGGCTTGC 
CTGGTTGGGC GGCAGTTCCT GAACCCAGCC AAGGCCTACC CTGGCCACGA GCTGGACCTT 
GTTGTACCTG TCTTCACATT TCTGCAGTTC TTCTTCTATG CTGGCTGGCT GAAGTGGTGC 
TGGGAGGTGG CAGAACAGCT CATCAACCCA TTTGGAGAGG ATGATGATGA CTTTGAGACC 
AACTGGATTG TTGACAGGAG TTTGCAGGTG TCCCTGTTGG CTGTGGACGA GATGCACCAG 
GACCTGCCCC CGATGGAGCG GGATATGTAC TGGAATGAAC CAGAACCACA ACCCCCCTAC 
ACGGCTGCTT CTGCCCAGTA TCGTCGAGCC TCCTTTTTAG GCTCCACTTT CAACATCAAT 
CTGCATAAGG AAGACATGGA GGAGGAAGAG GAAGGCACTC ACGCTGGCAT CATTGGCCGC 
TTTCTAGGGC TGCAGTCCCA CGACCACCAA CCCCCCAGGA CAAACTCAAA GACCAAACTA 
CTATGGCCCA AGAAAGAAGG CTTTCTCCAA GACGGCCAGC CCAAGAAACT CGGGGGTGCC 
AGGCAGACCT CTAGGGACAA GGAAGACAGC AAGGCCTGGG AGATTAAGGA GGAGGGTGCT 
TTTGAGACTG CTGCATTTTA TGGGAGGTCA GGCTACCACA GTGCCCCACA GACGCCCCTC 
AGCCACACAC CTATGGTCTT CCCATCTGGA CAGTCAGCAC CCTCAAGCCT ACGCAGAGTC 
TCAGGGATAG ACGGCGCTGT CAAAGACCAA AGCCTACAGC CTGGGATAAA GACCAGTTCT 
GAAGCACTCC CAGAGAATGT TGGGTTCTCA TTAGAGAACC CAGAATCGGG TCACGTGAAG 
AGGAAAACTG TCGAGTTTAA CCTGACAGAC ATGTCGGGGA CCCCTGAACA TCATCTCAGA 
GACTCAGATT TGAGCCAGTC AACTAGCAAC TTACACACTA TACTCAAGGG CCACGGAGAT 
CCCTATTGGG CCTTGGAAAA CAGGGATGAA GCACATTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_014120876.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976351.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe41041
Clone ID Related Accession (Same CDS sequence)	XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version	XM_014120876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976351.1
CDS	661..2415
Translation	MTVTYSSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLSYYTIRFIYRMALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKWCWEVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_014120876.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120876.2

ORF Insert Sequence:

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_022405083.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260791.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41041
Clone ID Related Accession (Same CDS sequence)	XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version	XM_022405083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260791.1
CDS	598..2352
Translation	MTVTYSSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLSYYTIRFIYRMALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKWCWEVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_022405083.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405083.1

ORF Insert Sequence:

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_014120876.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013976351.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe41041
Clone ID Related Accession (Same CDS sequence)	XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version	XM_014120876.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	bestrophin-1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013976351.1
CDS	701..2455
Translation	MTVTYSSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLSYYTIRFIYRMALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKWCWEVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_014120876.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014120876.1

ORF Insert Sequence:

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BEST1 ( XM_022405084.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260792.1 Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X3, mRNA.

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CloneID	OCe41041
Clone ID Related Accession (Same CDS sequence)	XM_014120876.2 , XM_022405083.1 , XM_014120876.1 , XM_022405084.1
Accession Version	XM_022405084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bestrophin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260792.1
CDS	173..1927
Translation	MTVTYSSQVANARLGSFSRLLLCWRGSIYKLLYGEFLIFLLSYYTIRFIYRMALTEEQQVMFEKLTLYCDSYIQLIPISFVLGFYVTLVVTRWWNQYENLPWPDRLMNLVSGLVEGRDEQGRLLRRTLIRYANLGNVLILRSVSAAVYKRFPSSQHLVKAGFMTPAECKHLEKLSLPHNTFWVPWVWFANLSMKAWIGGRIRDPVLLQSLLDEMNILRTQCGHLFAYDWISIPLVYTQVVTVAVYSFFLACLVGRQFLNPAKAYPGHELDLVVPVFTFLQFFFYAGWLKWCWEVAEQLINPFGEDDDDFETNWIVDRSLQVSLLAVDEMHQDLPPMERDMYWNEPEPQPPYTAASAQYRRASFLGSTFNINLHKEDMEEEEEGTHAGIIGRFLGLQSHDHQPPRTNSKTKLLWPKKEGFLQDGQPKKLGGARQTSRDKEDSKAWEIKEEGAFETAAFYGRSGYHSAPQTPLSHTPMVFPSGQSAPSSLRRVSGIDGAVKDQSLQPGIKTSSEALPENVGFSLENPESGHVKRKTVEFNLTDMSGTPEHHLRDSDLSQSTSNLHTILKGHGDPYWALENRDEAHS

Target ORF information:

RefSeq Version	XM_022405084.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris bestrophin 1 (BEST1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022405084.1

ORF Insert Sequence:

ATGACCGTCA CCTACTCAAG CCAAGTGGCT AATGCCCGCC TAGGCTCCTT CTCCCGCCTG 
CTGCTGTGCT GGCGAGGCAG CATCTACAAG CTGCTCTATG GCGAGTTCCT CATCTTCCTG 
CTCAGCTACT ATACTATTCG CTTCATTTAC AGGATGGCCC TCACGGAGGA ACAGCAGGTG 
ATGTTTGAGA AACTGACTCT GTATTGCGAC AGCTACATCC AGCTCATCCC CATTTCCTTC 
GTGCTGGGCT TCTACGTGAC TCTGGTCGTG ACCCGCTGGT GGAACCAGTA CGAGAACCTG 
CCGTGGCCCG ACCGCCTCAT GAACCTGGTG TCGGGCTTGG TGGAGGGCAG GGACGAGCAG 
GGCCGGCTGC TGCGCCGCAC GCTCATCCGC TACGCCAACC TGGGCAACGT GCTCATCCTG 
CGCAGCGTCA GCGCCGCCGT CTACAAGCGC TTCCCCAGCT CCCAGCACCT GGTGAAAGCA 
GGCTTTATGA CCCCCGCGGA ATGCAAGCAC TTAGAAAAGC TGAGCTTGCC GCACAACACG 
TTCTGGGTGC CCTGGGTGTG GTTTGCCAAC CTGTCAATGA AGGCGTGGAT TGGAGGTCGA 
ATCCGGGACC CTGTCCTGCT CCAGAGCCTG CTGGACGAGA TGAACATCTT GCGTACTCAG 
TGTGGACACC TGTTTGCCTA CGACTGGATC AGTATCCCGC TGGTGTACAC GCAGGTGGTG 
ACCGTGGCTG TATACAGCTT CTTCCTGGCT TGCCTGGTTG GGCGGCAGTT CCTGAACCCA 
GCCAAGGCCT ACCCTGGCCA CGAGCTGGAC CTTGTTGTAC CTGTCTTCAC ATTTCTGCAG 
TTCTTCTTCT ATGCTGGCTG GCTGAAGTGG TGCTGGGAGG TGGCAGAACA GCTCATCAAC 
CCATTTGGAG AGGATGATGA TGACTTTGAG ACCAACTGGA TTGTTGACAG GAGTTTGCAG 
GTGTCCCTGT TGGCTGTGGA CGAGATGCAC CAGGACCTGC CCCCGATGGA GCGGGATATG 
TACTGGAATG AACCAGAACC ACAACCCCCC TACACGGCTG CTTCTGCCCA GTATCGTCGA 
GCCTCCTTTT TAGGCTCCAC TTTCAACATC AATCTGCATA AGGAAGACAT GGAGGAGGAA 
GAGGAAGGCA CTCACGCTGG CATCATTGGC CGCTTTCTAG GGCTGCAGTC CCACGACCAC 
CAACCCCCCA GGACAAACTC AAAGACCAAA CTACTATGGC CCAAGAAAGA AGGCTTTCTC 
CAAGACGGCC AGCCCAAGAA ACTCGGGGGT GCCAGGCAGA CCTCTAGGGA CAAGGAAGAC 
AGCAAGGCCT GGGAGATTAA GGAGGAGGGT GCTTTTGAGA CTGCTGCATT TTATGGGAGG 
TCAGGCTACC ACAGTGCCCC ACAGACGCCC CTCAGCCACA CACCTATGGT CTTCCCATCT 
GGACAGTCAG CACCCTCAAG CCTACGCAGA GTCTCAGGGA TAGACGGCGC TGTCAAAGAC 
CAAAGCCTAC AGCCTGGGAT AAAGACCAGT TCTGAAGCAC TCCCAGAGAA TGTTGGGTTC 
TCATTAGAGA ACCCAGAATC GGGTCACGTG AAGAGGAAAA CTGTCGAGTT TAACCTGACA 
GACATGTCGG GGACCCCTGA ACATCATCTC AGAGACTCAG ATTTGAGCCA GTCAACTAGC 
AACTTACACA CTATACTCAA GGGCCACGGA GATCCCTATT GGGCCTTGGA AAACAGGGAT 
GAAGCACATT CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modeling the structural consequences of BEST1 missense mutations.
Advances in experimental medicine and biology723611-8(2012)
Guziewicz KE,Aguirre GD,Zangerl B

Assessment of canine BEST1 variations identifies new mutations and establishes an independent bestrophinopathy model (cmr3).
Molecular vision162791-804(2010 Dec)
Zangerl B,Wickstr?m K,Slavik J,Lindauer SJ,Ahonen S,Schelling C,Lohi H,Guziewicz KE,Aguirre GD

Multifocal retinal dysplasia in the German Spitz (Klein and Mittel) is not caused by mutations in BEST1.
Animal genetics41(3)333-4(2010 Jun)
Aguirre-Hern?ndez J,Sargan DR

Bestrophin gene mutations cause canine multifocal retinopathy: a novel animal model for best disease.
Investigative ophthalmology & visual science48(5)1959-67(2007 May)
Guziewicz KE,Zangerl B,Lindauer SJ,Mullins RF,Sandmeyer LS,Grahn BH,Stone EM,Acland GM,Aguirre GD

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BEST1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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