PC cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PC
Entrez Gene ID	483704
Full Name	pyruvate carboxylase
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005631415.2	XP_005631472.1	pyruvate carboxylase, mitochondrial isoform X1
XM_005631415.3	XP_005631472.1	pyruvate carboxylase, mitochondrial isoform X1
XM_540825.6	XP_540825.2	pyruvate carboxylase, mitochondrial isoform X2
XM_005631416.2	XP_005631473.1	pyruvate carboxylase, mitochondrial isoform X2
XM_005631416.3	XP_005631473.1	pyruvate carboxylase, mitochondrial isoform X2
XM_540825.5	XP_540825.2	pyruvate carboxylase, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PC	NP_071504.2
Canis lupus familiaris (dog)	PC	XP_540825.2
Bos taurus (cattle)	PC	NP_808815.2
Mus musculus (house mouse)	Pcx	NP_001156418.1
Caenorhabditis elegans (roundworm)	pyc-1	NP_001256376.1
Saccharomyces cerevisiae (baker's yeast)	PYC2	NP_009777.1
Kluyveromyces lactis	KLLA0C05764g	XP_452456.1
Eremothecium gossypii	AGOS_AAR162C	NP_982705.2
Magnaporthe oryzae (rice blast fungus)	MGG_07756	XP_003712969.1
Pan troglodytes (chimpanzee)	PC	XP_003952003.1
Neurospora crassa	NCU02505	XP_965636.1
Xenopus tropicalis (tropical clawed frog)	pc.2	NP_001096495.1
Schizosaccharomyces pombe (fission yeast)	pyr1	NP_595900.1
	PC	XP_001107814.1
Rattus norvegicus (Norway rat)	Pc	NP_036876.2
Danio rerio (zebrafish)	pcxb	NP_571625.1
Drosophila melanogaster (fruit fly)	CG1516	NP_724844.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004742	XP_001689096.1
Saccharomyces cerevisiae (baker's yeast)	PYC1	NP_011453.1

Gene Ontology

The following PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe20283	XM_005631415.3 Latest version!	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe20284	XM_540825.6 Latest version!	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe20284	XM_005631416.3 Latest version!	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
OCe20283	XM_005631415.2	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe20284	XM_005631416.2	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe20284	XM_540825.5	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PC ( XM_005631415.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005631472.1 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe20283
Clone ID Related Accession (Same CDS sequence)	XM_005631415.2 , XM_005631415.3
Accession Version	XM_005631415.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT 
GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT 
GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG 
GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC 
GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA 
AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC 
CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG 
ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT 
GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC 
TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC 
AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT 
GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG 
ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA 
CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG 
ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC 
ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC 
CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG 
ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT 
ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG 
CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT 
AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA 
GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG 
GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC 
CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC 
CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG 
GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT 
GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG 
GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA 
ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA 
AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA 
GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG 
GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT 
GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG 
AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG 
GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT 
GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA 
GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG 
CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG 
CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG 
GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC 
AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG 
GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT 
GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC 
GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG 
GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC 
CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG 
GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG 
CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA 
AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG 
CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC 
TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC 
CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT 
GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG 
AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG 
GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG 
AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG 
GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG 
GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG 
GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631472.1
CDS	108..3680
Translation	MASLGLCRPTATMLKFQTVRGGLRLLGIHRSSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEESYNRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGRHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGNAGGVVEAAISYTGDVADPNRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVASDSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQLLEKELTERHGEEVTPEDVLSAAIYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTTDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005631415.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631415.2

ORF Insert Sequence:

ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT 
GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT 
GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG 
GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC 
GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA 
AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC 
CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG 
ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT 
GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC 
TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC 
AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT 
GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG 
ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA 
CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG 
ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC 
ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC 
CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG 
ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT 
ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG 
CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT 
AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA 
GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG 
GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC 
CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC 
CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG 
GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT 
GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG 
GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA 
ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA 
AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA 
GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG 
GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT 
GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG 
AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG 
GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT 
GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA 
GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG 
CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG 
CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG 
GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC 
AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG 
GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT 
GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC 
GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG 
GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC 
CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG 
GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG 
CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA 
AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG 
CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC 
TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC 
CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT 
GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG 
AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG 
GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG 
AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG 
GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG 
GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG 
GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005631415.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005631472.1 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe20283
Clone ID Related Accession (Same CDS sequence)	XM_005631415.2 , XM_005631415.3
Accession Version	XM_005631415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pyruvate carboxylase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT 
GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT 
GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG 
GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC 
GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA 
AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC 
CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG 
ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT 
GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC 
TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC 
AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT 
GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG 
ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA 
CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG 
ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC 
ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC 
CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG 
ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT 
ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG 
CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT 
AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA 
GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG 
GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC 
CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC 
CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG 
GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT 
GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG 
GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA 
ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA 
AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA 
GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG 
GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT 
GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG 
AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG 
GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT 
GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA 
GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG 
CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG 
CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG 
GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC 
AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG 
GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT 
GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC 
GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG 
GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC 
CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG 
GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG 
CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA 
AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG 
CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC 
TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC 
CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT 
GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG 
AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG 
GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG 
AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG 
GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG 
GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG 
GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631472.1
CDS	134..3706
Translation	MASLGLCRPTATMLKFQTVRGGLRLLGIHRSSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEESYNRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGRHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGNAGGVVEAAISYTGDVADPNRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVASDSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQLLEKELTERHGEEVTPEDVLSAAIYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTTDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005631415.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631415.3

ORF Insert Sequence:

ATGGCCTCTC TGGGACTCTG CAGACCAACT GCCACGATGC TGAAGTTCCA AACCGTCCGT 
GGGGGCCTGA GGCTCCTGGG TATCCACCGA AGCTCCACTG CCCCTGCTGC CTCCCCAAAT 
GTCCGGCGCC TGGAGTACAA GCCCATCAAG AAAGTTATGG TGGCCAACAG AGGTGAGATT 
GCTATCCGTG TGTTCCGGGC CTGCACAGAG CTGGGCATCC GCACTGTAGC TGTCTACTCG 
GAGCAGGACA CAGGCCAGAT GCACCGGCAG AAAGCAGATG AAGCCTACCT CATCGGCCGC 
GGCCTTGCCC CAGTGCAGGC CTATCTGCAC ATCCCAGACA TCATCAAGGT GGCCAAGGAA 
AACAATGTGG ATGCAGTGCA CCCTGGCTAC GGGTTCCTGT CAGAGCGAGC AGACTTTGCC 
CAAGCCTGCC AGGATGCAGG GGTTCGGTTT ATTGGGCCGA GCCCTGAGGT GGTCCGCAAG 
ATGGGCGATA AGGTGGAGGC CCGGGCCATC GCCATTGCTG CAGGTGTCCC TGTGGTCCCT 
GGCACAGATG CCCCCATCAC TTCCTTGCAC GAGGCCCATG AATTCTCTAA CACCTATGGC 
TTCCCCATTA TCTTCAAGGC CGCCTATGGA GGTGGGGGAC GTGGCATGAG GGTCGTGCAC 
AGTTATGAGG AGCTGGAGGA AAGCTATAAC CGGGCCTACT CGGAGGCCCT GGCCGCCTTT 
GGGAATGGGG CGCTGTTTGT GGAGAAGTTC ATTGAGAAGC CACGCCACAT CGAGGTGCAG 
ATCCTGGGAG ACCAGTACGG GAATATTCTG CACCTCTATG AGCGAGACTG TTCCATCCAA 
CGGCGGCACC AGAAAGTGGT TGAGATTGCT CCTGCAGCCC ACCTGGATCC CCAGCTTCGG 
ACACGGCTCA CCAGTGACTC TGTCAAACTT GCTAAGCAGG TGGGCTACGA GAACGCAGGC 
ACGGTGGAGT TCCTGGTGGA CCGGCATGGC AGGCACTACT TCATCGAGGT CAACTCACGC 
CTGCAGGTGG AGCACACAGT CACCGAGGAG ATCACAGATG TGGATCTGGT TCACGCCCAG 
ATCCATGTGG CCGAGGGCCG GAGCCTGCCT GACCTGGGCC TGCGGCAGGA GAATATCCGT 
ATCAACGGTT GTGCCATTCA GTGCCGGGTC ACCACCGAGG ACCCTGCACG CAGCTTCCAG 
CCAGACACTG GCCGAATCGA GGTGTTCCGG AGCGGAGAGG GCATGGGCAT CCGCCTGGAT 
AATGCATCTG CCTTCCAAGG AGCTGTCATC TCCCCGCACT ATGACTCCCT GCTCGTCAAA 
GTCATTGCTC ATGGCAAAGA CCACCCCACG GCCGCCACCA AGATGAGCAG AGCCCTTGCG 
GAGTTCCGAG TCCGAGGTGT AAAGACCAAC ATCCCCTTCC TGCAGAATGT GCTCAACAAC 
CAGCAGTTCC TGGCAGGCAC CGTGGACACC CAGTTCATCG ATGAGAACCC GGAGCTTTTC 
CAGCTGCGGC CTGCGCAGAA CCGGGCTCAG AAGCTGCTGC ACTACCTCGG TCATGTCATG 
GTGAACGGAC CAACCACCCC AATCCCTGTC AAGGCCAGCC CCAGCCCCAC GGACCCTGTT 
GTCCCTGCGG TGCCCATAGG CCCACCCCCA GCTGGTTTCA GAGACATCCT GCTGCGGGAG 
GGGCCTGAGG GCTTTGCTCG AGCTGTGAGG AACCACCAGG GGCTGCTGCT CATGGACACA 
ACCTTCAGGG ATGCCCACCA GTCGCTGCTG GCCACTCGTG TGCGAACCCA TGATCTCAAA 
AAGATTGCCC CCTACGTTGC CCACAACTTC AGCAAGCTCT TCAGCATAGA GAACTGGGGA 
GGAGCCACGT TTGATGTTGC CATGCGCTTT CTGTACGAGT GCCCCTGGCG GCGGCTGCAG 
GAGCTCCGGG AGCTCATCCC CAACATCCCG TTTCAGATGC TGCTGCGGGG GGCCAATGCT 
GTGGGCTACA CCAACTACCC TGACAATGTG GTGTTCAAGT TCTGTGAGGT AGCCAAGGAG 
AATGGCATGG ACGTCTTCCG GGTTTTTGAC TCCCTCAACT ACATGCCAAA TCTGCTACTG 
GGCATGGAGG CTGCAGGCAA TGCTGGTGGC GTGGTGGAGG CTGCCATCTC CTACACTGGT 
GACGTGGCCG ACCCCAACCG CACCAAATAC TCACTGCAGT ACTACATGGG CTTGGCGGAA 
GAGCTGGTGC GAGCTGGCAC CCATATCCTG TGTATCAAGG ACATGGCAGG GCTGCTAAAG 
CCTACAGCCT GCACCATGCT GGTCAGCTCC CTCAGGGACC GGTTCCCCGA CCTTCCACTG 
CACATCCACA CCCATGACAC ATCAGGGGCA GGTGTGGCAG CTATGTTGGC CTGTGCCCAG 
GCTGGGGCTG ATGTGGTAGA TGTGGCATCT GACTCCATGT CCGGGATGAC TTCACAGCCC 
AGCATGGGGG CCCTGGTGGC CTGCACCCGA GGGACTCCCC TGGACACAGG GGTGCCCCTG 
GAACGTGTGT TTGACTACAG TGAGTACTGG GAGGGGGCCC GGGGATTGTA TGCGGCCTTT 
GACTGCACGG CCACCATGAA GTCTGGCAAT TCGGACGTGT ATGAGAACGA GATCCCAGGC 
GGCCAGTACA CCAATCTGCA CTTCCAGGCA CACAGCATGG GGCTTGGCTC CAAGTTCAAG 
GAAGTCAAGA AGGCCTATGT GGAGGCCAAC CAGATGCTGG GCGATCTCAT CAAGGTGACC 
CCGTCCTCCA AGATCGTGGG CGACCTGGCC CAGTTCATGG TACAGAATGG GCTGAGCCGG 
GCAGAGGCTG AAGCCCAGGC AGAAGAATTG TCCTTCCCCC GTTCCGTGGT GGAGTTCCTG 
CAGGGCTACA TTGGCATTCC CCACGGGGGC TTCCCTGAGC CCCTTCGCTC TAAGGTGCTA 
AAGGACCTGC CAAGGGTAGA AGGGCGGCCA GGAGCCTCCC TCCCCCCACT GGATCTGCAG 
CTGCTGGAGA AGGAGCTGAC TGAACGGCAT GGGGAGGAGG TGACCCCAGA AGATGTGCTC 
TCAGCAGCCA TATATCCAGA CGTCTTTGCC CACTTCAAGG ACTTCACAGC TACCTTTGGC 
CCCCTGGATA GCCTCAATAC CCGCCTCTTC CTGCAGGGAC CCAAAATTGC AGAGGAGTTT 
GAGGTGGAGC TGGAGCGGGG CAAGACACTG CACATCAAGG CTCTGGCCAT AAGTGACCTG 
AACCGGGCTG GCCAGAGGCA GGTCTTCTTT GAGCTCAATG GACAGCTGCG GTCCATTCTG 
GTCAAGGACA CTCAGGCCAT GAAGGAAATG CACTTCCACC CCAAGGCCCT GAAGGATGTG 
AAGGGCCAGA TTGGGGCACC CATGCCTGGG AAGGTGATAG ACATCAAGGT GGCAGCTGGG 
GCCAAGGTGG CCAAGGGCCA GCCCCTCTGT GTGCTCAGTG CCATGAAGAT GGAGACTGTG 
GTGACTTCAC CCATGGAGGG CACTGTCCGC AAGGTCCACG TAACCACAGA CATGACACTG 
GAGGGCGATG ACCTCATCCT GGAGATTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_540825.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_540825.2 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.

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CloneID	OCe20284
Clone ID Related Accession (Same CDS sequence)	XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
Accession Version	XM_540825.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540825.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_540825.2
CDS	187..3723
Translation	MLKFQTVRGGLRLLGIHRSSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEESYNRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGRHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGNAGGVVEAAISYTGDVADPNRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVASDSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQLLEKELTERHGEEVTPEDVLSAAIYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTTDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_540825.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_540825.6

ORF Insert Sequence:

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005631416.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005631473.1 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.

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CloneID	OCe20284
Clone ID Related Accession (Same CDS sequence)	XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
Accession Version	XM_005631416.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005631416.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631473.1
CDS	86..3622
Translation	MLKFQTVRGGLRLLGIHRSSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEESYNRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGRHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGNAGGVVEAAISYTGDVADPNRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVASDSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQLLEKELTERHGEEVTPEDVLSAAIYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTTDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005631416.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631416.2

ORF Insert Sequence:

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_005631416.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005631473.1 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe20284
Clone ID Related Accession (Same CDS sequence)	XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
Accession Version	XM_005631416.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006600.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005631416.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005631473.1
CDS	144..3680
Translation	MLKFQTVRGGLRLLGIHRSSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEESYNRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGRHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGNAGGVVEAAISYTGDVADPNRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVASDSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQLLEKELTERHGEEVTPEDVLSAAIYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTTDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_005631416.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005631416.3

ORF Insert Sequence:

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PC ( XM_540825.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_540825.2 Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.

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CloneID	OCe20284
Clone ID Related Accession (Same CDS sequence)	XM_540825.6 , XM_005631416.2 , XM_005631416.3 , XM_540825.5
Accession Version	XM_540825.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	pyruvate carboxylase, mitochondrial isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726092.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540825.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_540825.2
CDS	161..3697
Translation	MLKFQTVRGGLRLLGIHRSSTAPAASPNVRRLEYKPIKKVMVANRGEIAIRVFRACTELGIRTVAVYSEQDTGQMHRQKADEAYLIGRGLAPVQAYLHIPDIIKVAKENNVDAVHPGYGFLSERADFAQACQDAGVRFIGPSPEVVRKMGDKVEARAIAIAAGVPVVPGTDAPITSLHEAHEFSNTYGFPIIFKAAYGGGGRGMRVVHSYEELEESYNRAYSEALAAFGNGALFVEKFIEKPRHIEVQILGDQYGNILHLYERDCSIQRRHQKVVEIAPAAHLDPQLRTRLTSDSVKLAKQVGYENAGTVEFLVDRHGRHYFIEVNSRLQVEHTVTEEITDVDLVHAQIHVAEGRSLPDLGLRQENIRINGCAIQCRVTTEDPARSFQPDTGRIEVFRSGEGMGIRLDNASAFQGAVISPHYDSLLVKVIAHGKDHPTAATKMSRALAEFRVRGVKTNIPFLQNVLNNQQFLAGTVDTQFIDENPELFQLRPAQNRAQKLLHYLGHVMVNGPTTPIPVKASPSPTDPVVPAVPIGPPPAGFRDILLREGPEGFARAVRNHQGLLLMDTTFRDAHQSLLATRVRTHDLKKIAPYVAHNFSKLFSIENWGGATFDVAMRFLYECPWRRLQELRELIPNIPFQMLLRGANAVGYTNYPDNVVFKFCEVAKENGMDVFRVFDSLNYMPNLLLGMEAAGNAGGVVEAAISYTGDVADPNRTKYSLQYYMGLAEELVRAGTHILCIKDMAGLLKPTACTMLVSSLRDRFPDLPLHIHTHDTSGAGVAAMLACAQAGADVVDVASDSMSGMTSQPSMGALVACTRGTPLDTGVPLERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGIPHGGFPEPLRSKVLKDLPRVEGRPGASLPPLDLQLLEKELTERHGEEVTPEDVLSAAIYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAISDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVAAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTTDMTLEGDDLILEIE

Target ORF information:

RefSeq Version	XM_540825.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris pyruvate carboxylase (PC), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_540825.5

ORF Insert Sequence:

ATGCTGAAGT TCCAAACCGT CCGTGGGGGC CTGAGGCTCC TGGGTATCCA CCGAAGCTCC 
ACTGCCCCTG CTGCCTCCCC AAATGTCCGG CGCCTGGAGT ACAAGCCCAT CAAGAAAGTT 
ATGGTGGCCA ACAGAGGTGA GATTGCTATC CGTGTGTTCC GGGCCTGCAC AGAGCTGGGC 
ATCCGCACTG TAGCTGTCTA CTCGGAGCAG GACACAGGCC AGATGCACCG GCAGAAAGCA 
GATGAAGCCT ACCTCATCGG CCGCGGCCTT GCCCCAGTGC AGGCCTATCT GCACATCCCA 
GACATCATCA AGGTGGCCAA GGAAAACAAT GTGGATGCAG TGCACCCTGG CTACGGGTTC 
CTGTCAGAGC GAGCAGACTT TGCCCAAGCC TGCCAGGATG CAGGGGTTCG GTTTATTGGG 
CCGAGCCCTG AGGTGGTCCG CAAGATGGGC GATAAGGTGG AGGCCCGGGC CATCGCCATT 
GCTGCAGGTG TCCCTGTGGT CCCTGGCACA GATGCCCCCA TCACTTCCTT GCACGAGGCC 
CATGAATTCT CTAACACCTA TGGCTTCCCC ATTATCTTCA AGGCCGCCTA TGGAGGTGGG 
GGACGTGGCA TGAGGGTCGT GCACAGTTAT GAGGAGCTGG AGGAAAGCTA TAACCGGGCC 
TACTCGGAGG CCCTGGCCGC CTTTGGGAAT GGGGCGCTGT TTGTGGAGAA GTTCATTGAG 
AAGCCACGCC ACATCGAGGT GCAGATCCTG GGAGACCAGT ACGGGAATAT TCTGCACCTC 
TATGAGCGAG ACTGTTCCAT CCAACGGCGG CACCAGAAAG TGGTTGAGAT TGCTCCTGCA 
GCCCACCTGG ATCCCCAGCT TCGGACACGG CTCACCAGTG ACTCTGTCAA ACTTGCTAAG 
CAGGTGGGCT ACGAGAACGC AGGCACGGTG GAGTTCCTGG TGGACCGGCA TGGCAGGCAC 
TACTTCATCG AGGTCAACTC ACGCCTGCAG GTGGAGCACA CAGTCACCGA GGAGATCACA 
GATGTGGATC TGGTTCACGC CCAGATCCAT GTGGCCGAGG GCCGGAGCCT GCCTGACCTG 
GGCCTGCGGC AGGAGAATAT CCGTATCAAC GGTTGTGCCA TTCAGTGCCG GGTCACCACC 
GAGGACCCTG CACGCAGCTT CCAGCCAGAC ACTGGCCGAA TCGAGGTGTT CCGGAGCGGA 
GAGGGCATGG GCATCCGCCT GGATAATGCA TCTGCCTTCC AAGGAGCTGT CATCTCCCCG 
CACTATGACT CCCTGCTCGT CAAAGTCATT GCTCATGGCA AAGACCACCC CACGGCCGCC 
ACCAAGATGA GCAGAGCCCT TGCGGAGTTC CGAGTCCGAG GTGTAAAGAC CAACATCCCC 
TTCCTGCAGA ATGTGCTCAA CAACCAGCAG TTCCTGGCAG GCACCGTGGA CACCCAGTTC 
ATCGATGAGA ACCCGGAGCT TTTCCAGCTG CGGCCTGCGC AGAACCGGGC TCAGAAGCTG 
CTGCACTACC TCGGTCATGT CATGGTGAAC GGACCAACCA CCCCAATCCC TGTCAAGGCC 
AGCCCCAGCC CCACGGACCC TGTTGTCCCT GCGGTGCCCA TAGGCCCACC CCCAGCTGGT 
TTCAGAGACA TCCTGCTGCG GGAGGGGCCT GAGGGCTTTG CTCGAGCTGT GAGGAACCAC 
CAGGGGCTGC TGCTCATGGA CACAACCTTC AGGGATGCCC ACCAGTCGCT GCTGGCCACT 
CGTGTGCGAA CCCATGATCT CAAAAAGATT GCCCCCTACG TTGCCCACAA CTTCAGCAAG 
CTCTTCAGCA TAGAGAACTG GGGAGGAGCC ACGTTTGATG TTGCCATGCG CTTTCTGTAC 
GAGTGCCCCT GGCGGCGGCT GCAGGAGCTC CGGGAGCTCA TCCCCAACAT CCCGTTTCAG 
ATGCTGCTGC GGGGGGCCAA TGCTGTGGGC TACACCAACT ACCCTGACAA TGTGGTGTTC 
AAGTTCTGTG AGGTAGCCAA GGAGAATGGC ATGGACGTCT TCCGGGTTTT TGACTCCCTC 
AACTACATGC CAAATCTGCT ACTGGGCATG GAGGCTGCAG GCAATGCTGG TGGCGTGGTG 
GAGGCTGCCA TCTCCTACAC TGGTGACGTG GCCGACCCCA ACCGCACCAA ATACTCACTG 
CAGTACTACA TGGGCTTGGC GGAAGAGCTG GTGCGAGCTG GCACCCATAT CCTGTGTATC 
AAGGACATGG CAGGGCTGCT AAAGCCTACA GCCTGCACCA TGCTGGTCAG CTCCCTCAGG 
GACCGGTTCC CCGACCTTCC ACTGCACATC CACACCCATG ACACATCAGG GGCAGGTGTG 
GCAGCTATGT TGGCCTGTGC CCAGGCTGGG GCTGATGTGG TAGATGTGGC ATCTGACTCC 
ATGTCCGGGA TGACTTCACA GCCCAGCATG GGGGCCCTGG TGGCCTGCAC CCGAGGGACT 
CCCCTGGACA CAGGGGTGCC CCTGGAACGT GTGTTTGACT ACAGTGAGTA CTGGGAGGGG 
GCCCGGGGAT TGTATGCGGC CTTTGACTGC ACGGCCACCA TGAAGTCTGG CAATTCGGAC 
GTGTATGAGA ACGAGATCCC AGGCGGCCAG TACACCAATC TGCACTTCCA GGCACACAGC 
ATGGGGCTTG GCTCCAAGTT CAAGGAAGTC AAGAAGGCCT ATGTGGAGGC CAACCAGATG 
CTGGGCGATC TCATCAAGGT GACCCCGTCC TCCAAGATCG TGGGCGACCT GGCCCAGTTC 
ATGGTACAGA ATGGGCTGAG CCGGGCAGAG GCTGAAGCCC AGGCAGAAGA ATTGTCCTTC 
CCCCGTTCCG TGGTGGAGTT CCTGCAGGGC TACATTGGCA TTCCCCACGG GGGCTTCCCT 
GAGCCCCTTC GCTCTAAGGT GCTAAAGGAC CTGCCAAGGG TAGAAGGGCG GCCAGGAGCC 
TCCCTCCCCC CACTGGATCT GCAGCTGCTG GAGAAGGAGC TGACTGAACG GCATGGGGAG 
GAGGTGACCC CAGAAGATGT GCTCTCAGCA GCCATATATC CAGACGTCTT TGCCCACTTC 
AAGGACTTCA CAGCTACCTT TGGCCCCCTG GATAGCCTCA ATACCCGCCT CTTCCTGCAG 
GGACCCAAAA TTGCAGAGGA GTTTGAGGTG GAGCTGGAGC GGGGCAAGAC ACTGCACATC 
AAGGCTCTGG CCATAAGTGA CCTGAACCGG GCTGGCCAGA GGCAGGTCTT CTTTGAGCTC 
AATGGACAGC TGCGGTCCAT TCTGGTCAAG GACACTCAGG CCATGAAGGA AATGCACTTC 
CACCCCAAGG CCCTGAAGGA TGTGAAGGGC CAGATTGGGG CACCCATGCC TGGGAAGGTG 
ATAGACATCA AGGTGGCAGC TGGGGCCAAG GTGGCCAAGG GCCAGCCCCT CTGTGTGCTC 
AGTGCCATGA AGATGGAGAC TGTGGTGACT TCACCCATGG AGGGCACTGT CCGCAAGGTC 
CACGTAACCA CAGACATGAC ACTGGAGGGC GATGACCTCA TCCTGGAGAT TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PC cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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電話

FAX

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