GAA cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAA
Entrez Gene ID	483352
Full Name	glucosidase alpha, acid
General protein information	Preferred Names glucosidase alpha, acid Names lysosomal alpha-glucosidase glucosidase, alpha; acid (Pompe disease, glycogen storage disease type II)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005624023.2	XP_005624080.1	lysosomal alpha-glucosidase isoform X2
XM_845556.5	XP_850649.1	lysosomal alpha-glucosidase isoform X2
XM_005624023.3	XP_005624080.1	lysosomal alpha-glucosidase isoform X2
XM_845556.4	XP_850649.1	lysosomal alpha-glucosidase isoform X2
XM_022422643.1	XP_022278351.1	lysosomal alpha-glucosidase isoform X1
XM_005624022.2	XP_005624079.1	lysosomal alpha-glucosidase isoform X1
XM_005624022.3	XP_005624079.1	lysosomal alpha-glucosidase isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC422082	XP_420085.4
Schizosaccharomyces pombe (fission yeast)	SPAC30D11.01c	NP_593216.2
Canis lupus familiaris (dog)	GAA	XP_005624079.1
Mus musculus (house mouse)	Gaa	NP_032090.3
Pan troglodytes (chimpanzee)	GAA	XP_001160653.1
Rattus norvegicus (Norway rat)	Gaa	NP_954549.1
Danio rerio (zebrafish)	gaa	XP_001921957.2
Schizosaccharomyces pombe (fission yeast)	agl1	NP_593996.1
Oryza sativa (rice)	Os01g0130400	NP_001041922.1
Bos taurus (cattle)	GAA	NP_776338.1
Schizosaccharomyces pombe (fission yeast)	SPAC1039.11c	XP_001713119.1
Neurospora crassa	NCU09281	XP_958500.1
Xenopus tropicalis (tropical clawed frog)	gaa	XP_002932561.2
Homo sapiens (human)	GAA	NP_001073272.1
	GAA	XP_001109980.1
Magnaporthe oryzae (rice blast fungus)	MGG_10662	XP_003710096.1
Arabidopsis thaliana (thale cress)	XYL1	NP_177023.1
Arabidopsis thaliana (thale cress)	AT3G45940	NP_190180.1

Gene Ontology

The following GAA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe11090	XM_022422643.1 Latest version!	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe11090	XM_005624022.3 Latest version!	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe11091	XM_845556.5 Latest version!	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe11091	XM_005624023.3 Latest version!	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
OCe11091	XM_005624023.2	Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe11091	XM_845556.4	Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe11090	XM_005624022.2	Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAA ( XM_022422643.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022278351.1 Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe11090
Clone ID Related Accession (Same CDS sequence)	XM_022422643.1 , XM_005624022.2 , XM_005624022.3
Accession Version	XM_022422643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC ACCAACGACC GATGATTAAT CAGCTGTGCT CGGGCCTCCA GGACCCAGCC 
CGCGGCCCGC AGGTGCCACC GACCATGGAC GCGAGGTGGC CTCCCAGCTC CCGGCCCCTG 
CTGGGGGTCT GCACCCTCGT CTGCCTGGCC ACCGCTGCCT TCCTGGGCCA CGTCCTGCTC 
CGTGACTCCT TGGTGGCTCC CCAAGAGCAA CGGCGCTTCT CCCAAGGGCC CGAGCAGCTT 
TATCATGCTC ACCAGCAAGG AGCCCGTAAC CCAGGCCCCC AGCCCAGCCC AGGGCACCCC 
GGCAGGCCCC GAGCGGCACC CACACAGTGT GATGTGCCCC CCAACAGCCG TTTCGACTGT 
GCCCCGGACA AGGCCATCAC CCAGCAGCAG TGTGAGGCGC GGGGCTGCTG CTACAGGCCC 
GCGACGCCCT GGCCCCAGCA CCCCCGGATG GGACAGCCCT GGTGCTTCTT CCCACCCAGC 
TACCCGAGTT ACAAGCTGGA GAACCTGACC ACCACGGAGA CGGGCTACAC GGCCGCTCTG 
ACCCGTAGCA CCCCAACCTT CTTCCCCAAG GACATTCTGG CCTTACGGTT GGACGTGCTG 
CTGGAGACGG AGAGCCGGCT CCACTTCACG ATCAAAGATC CCACCAACAG GCGCTATGAG 
GTGCCCCTGG AGACCCCGCG GGCCCACGGC CGCGCCTTGG CCACACTCTA CAGCGTGGAG 
TTCCAGGAGG AGCCCTTCGG GGTGGTCGTG CGCCGGAAGC TGGACGGCCG GGTGCTGCTG 
AACACGACGG TGGCCCCCCT GTTCTTCGCC GACCAGTTTC TGCAGCTGTC CACCTCCCTG 
CCGTCCCAGT ACATCGCTGG CCTTGCCGAG CATCTCGGCT CCCTGATGCT CAGCACCAAC 
TGGACCAGGG TCACCCTCTG GAACCGGGAC ATCGCCCCCT CGCCCAACGT GAACCTGTAC 
GGGTCCCACC CTTTCTACCT GGCGCTGGAG GATGGCGGGT CAGCTCATGG GGTCTTCCTG 
CTGAACAGCA ATGCCATGGA CGTGGTCCTG CAGCCGAGCC CGGCCCTCAG CTGGAGGTCG 
ACAGGCGGGA TCCTGGACGT GTACGTCTTC CTGGGCCCGG AGCCCAAGAG CGTGGTACAG 
CAGTACCTGG AAGTCGTGGG CTCCCCGTTC ATGCCACCCT ACTGGGGCCT GGGCTTCCAC 
CTGTGCCGCT GGGGATACTC GTCCACAGCC ATCACCCGCC AGGTGGTAGA GAACATGACC 
CGGGCCCACT TCCCCCTGGA CACGCAGTGG AACGACCTGG ATTACATGGA CGCCAGGAGG 
GACTTTACTT TCAACAAAGA CGGCTTCAGG GACTTCCCGG CCATGGTGCA GGAGCTCCAC 
CGGGGCGGCC GGCGGTACGT GATGATCGTG GACCCGGCCA TCAGCAGCTC CAGCCCCCCT 
GGCAGCTACC GACCCTATGA CGAGGGTCTG CGGAGAAAGG TTTTCATCAC CAATGAGACG 
GGGCAGCCGC TGATTGGGAA GGTGTGGCCC GGGTTCACTG TCTTCCCCGA CTTCACCAGC 
CCCACGGCCC TGGACTGGTG GCAGGACATG GTGTCTGAGT TCCATGCCCA GGTGCCCTTC 
GACGGCATGT GGATTGACAT GAACGAGCCA TCCAACTTCG TGAGGGGCTC GGTGTATGGC 
TGCCCCGACA ATGACCTGGA GAACCCGCCC TACGTGCCCG GGGTGGTTGG CGGGACCCTG 
CGGGCAGCCA CCATCTGTGC CTCCAGCCGC CAGCTGCTCT CCACGCACTA CAACCTGCAC 
AACCTGTATG GCCTGACGGA AGCCATCGCC TCCCACAGGG CCCTCGTGAA GGCTCGGGGG 
ACGCGCCCCT TTGTGATCTC CCGCTCAACC TTCGCTGGCC ACGGCCAGTA TGCCGGCCAC 
TGGACAGGGG ACGTGTGGAG CAGCTGGGAG CAGCTCTCGT ACTCTGTACC AGAAATCCTG 
CTGTTCAACC TGCTGGGGGT GCCGCTGGTC GGGGCCGACG TCTGTGGCTT CCTGGGCAAC 
ACGTCAGAGG AGCTGTGCGT GCGCTGGACC CAGCTGGGGG CCTTCTATCC GTTCATGCGG 
AACCACAACG ACCTCAACAG CCTGCCCCAG GAGCCCTACA GGTTCAGCGC GACGGCGCAG 
GAAGCCATGA GGAAGGCGCT CGCCCTGCGC TACTCGCTGC TGCCCCACCT CTACACGCTC 
TTCCACCGGG CACACGTGGG GGGCGAGACC GTGGCCCGGC CCCTCTTCCT CGAGTTCCCT 
GAAGACCCCC ACACCTGGAC CGTGGACCGC CAGCTCCTGT GGGGGGAGGC TCTGCTCATC 
ACCCCCGTGC TCGAGGCCGG GAAGGTCGAA GTGACTGGCT ACTTCCCCGC TGGCACATGG 
TACGACCTGC AGACGGTGCC CGTAGGGGCC TTCGGCAGCC TCCCGCCCCC ACCTCCAGCT 
CCGCTCATGT CCACCATCCA CAGCAAGGGG CAGTGGGTGA CGCTGCCAGC CCCGCTGGAC 
ACCATCAACG TCCACCTCCG GGCTGGGCAC ATCATCCCCC TGCAGGGCCC TGGCCTCACG 
ACCACAGAGT CCCGAAAGCA GCCCATGGCC CTGGTCGCAG CCCTGGGCAC AAACGGGGAA 
GCCCGAGGGG AGCTGTTCTG GGACGACGGG GAGAGCCTCG GGGTGCTGGA GCGCGGGGCC 
TACACGGAGG TGGTCTTCCT GGCCAGGAAC GACACCATCG TGAACGAGCT GGTACGTGTG 
ACTAGCGAGG GGGCCGGCCT GCAGCTGAGG AAGGTGACCG TCCTGGGGGT GGCCGCGGGC 
CCCAGGCAGG TCCTCTGCAA CGGCGTCCCT GTTGCCTTCA CCTACAACCC TGACACCAAG 
ACCCTGGACA TCCCCGTGTC ACTGACGATG GGAGAACAGT TTCTCATCAG CTGGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278351.1
CDS	200..3139
Translation	MGHHQRPMINQLCSGLQDPARGPQVPPTMDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_022422643.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022422643.1

ORF Insert Sequence:

ATGGGGCATC ACCAACGACC GATGATTAAT CAGCTGTGCT CGGGCCTCCA GGACCCAGCC 
CGCGGCCCGC AGGTGCCACC GACCATGGAC GCGAGGTGGC CTCCCAGCTC CCGGCCCCTG 
CTGGGGGTCT GCACCCTCGT CTGCCTGGCC ACCGCTGCCT TCCTGGGCCA CGTCCTGCTC 
CGTGACTCCT TGGTGGCTCC CCAAGAGCAA CGGCGCTTCT CCCAAGGGCC CGAGCAGCTT 
TATCATGCTC ACCAGCAAGG AGCCCGTAAC CCAGGCCCCC AGCCCAGCCC AGGGCACCCC 
GGCAGGCCCC GAGCGGCACC CACACAGTGT GATGTGCCCC CCAACAGCCG TTTCGACTGT 
GCCCCGGACA AGGCCATCAC CCAGCAGCAG TGTGAGGCGC GGGGCTGCTG CTACAGGCCC 
GCGACGCCCT GGCCCCAGCA CCCCCGGATG GGACAGCCCT GGTGCTTCTT CCCACCCAGC 
TACCCGAGTT ACAAGCTGGA GAACCTGACC ACCACGGAGA CGGGCTACAC GGCCGCTCTG 
ACCCGTAGCA CCCCAACCTT CTTCCCCAAG GACATTCTGG CCTTACGGTT GGACGTGCTG 
CTGGAGACGG AGAGCCGGCT CCACTTCACG ATCAAAGATC CCACCAACAG GCGCTATGAG 
GTGCCCCTGG AGACCCCGCG GGCCCACGGC CGCGCCTTGG CCACACTCTA CAGCGTGGAG 
TTCCAGGAGG AGCCCTTCGG GGTGGTCGTG CGCCGGAAGC TGGACGGCCG GGTGCTGCTG 
AACACGACGG TGGCCCCCCT GTTCTTCGCC GACCAGTTTC TGCAGCTGTC CACCTCCCTG 
CCGTCCCAGT ACATCGCTGG CCTTGCCGAG CATCTCGGCT CCCTGATGCT CAGCACCAAC 
TGGACCAGGG TCACCCTCTG GAACCGGGAC ATCGCCCCCT CGCCCAACGT GAACCTGTAC 
GGGTCCCACC CTTTCTACCT GGCGCTGGAG GATGGCGGGT CAGCTCATGG GGTCTTCCTG 
CTGAACAGCA ATGCCATGGA CGTGGTCCTG CAGCCGAGCC CGGCCCTCAG CTGGAGGTCG 
ACAGGCGGGA TCCTGGACGT GTACGTCTTC CTGGGCCCGG AGCCCAAGAG CGTGGTACAG 
CAGTACCTGG AAGTCGTGGG CTCCCCGTTC ATGCCACCCT ACTGGGGCCT GGGCTTCCAC 
CTGTGCCGCT GGGGATACTC GTCCACAGCC ATCACCCGCC AGGTGGTAGA GAACATGACC 
CGGGCCCACT TCCCCCTGGA CACGCAGTGG AACGACCTGG ATTACATGGA CGCCAGGAGG 
GACTTTACTT TCAACAAAGA CGGCTTCAGG GACTTCCCGG CCATGGTGCA GGAGCTCCAC 
CGGGGCGGCC GGCGGTACGT GATGATCGTG GACCCGGCCA TCAGCAGCTC CAGCCCCCCT 
GGCAGCTACC GACCCTATGA CGAGGGTCTG CGGAGAAAGG TTTTCATCAC CAATGAGACG 
GGGCAGCCGC TGATTGGGAA GGTGTGGCCC GGGTTCACTG TCTTCCCCGA CTTCACCAGC 
CCCACGGCCC TGGACTGGTG GCAGGACATG GTGTCTGAGT TCCATGCCCA GGTGCCCTTC 
GACGGCATGT GGATTGACAT GAACGAGCCA TCCAACTTCG TGAGGGGCTC GGTGTATGGC 
TGCCCCGACA ATGACCTGGA GAACCCGCCC TACGTGCCCG GGGTGGTTGG CGGGACCCTG 
CGGGCAGCCA CCATCTGTGC CTCCAGCCGC CAGCTGCTCT CCACGCACTA CAACCTGCAC 
AACCTGTATG GCCTGACGGA AGCCATCGCC TCCCACAGGG CCCTCGTGAA GGCTCGGGGG 
ACGCGCCCCT TTGTGATCTC CCGCTCAACC TTCGCTGGCC ACGGCCAGTA TGCCGGCCAC 
TGGACAGGGG ACGTGTGGAG CAGCTGGGAG CAGCTCTCGT ACTCTGTACC AGAAATCCTG 
CTGTTCAACC TGCTGGGGGT GCCGCTGGTC GGGGCCGACG TCTGTGGCTT CCTGGGCAAC 
ACGTCAGAGG AGCTGTGCGT GCGCTGGACC CAGCTGGGGG CCTTCTATCC GTTCATGCGG 
AACCACAACG ACCTCAACAG CCTGCCCCAG GAGCCCTACA GGTTCAGCGC GACGGCGCAG 
GAAGCCATGA GGAAGGCGCT CGCCCTGCGC TACTCGCTGC TGCCCCACCT CTACACGCTC 
TTCCACCGGG CACACGTGGG GGGCGAGACC GTGGCCCGGC CCCTCTTCCT CGAGTTCCCT 
GAAGACCCCC ACACCTGGAC CGTGGACCGC CAGCTCCTGT GGGGGGAGGC TCTGCTCATC 
ACCCCCGTGC TCGAGGCCGG GAAGGTCGAA GTGACTGGCT ACTTCCCCGC TGGCACATGG 
TACGACCTGC AGACGGTGCC CGTAGGGGCC TTCGGCAGCC TCCCGCCCCC ACCTCCAGCT 
CCGCTCATGT CCACCATCCA CAGCAAGGGG CAGTGGGTGA CGCTGCCAGC CCCGCTGGAC 
ACCATCAACG TCCACCTCCG GGCTGGGCAC ATCATCCCCC TGCAGGGCCC TGGCCTCACG 
ACCACAGAGT CCCGAAAGCA GCCCATGGCC CTGGTCGCAG CCCTGGGCAC AAACGGGGAA 
GCCCGAGGGG AGCTGTTCTG GGACGACGGG GAGAGCCTCG GGGTGCTGGA GCGCGGGGCC 
TACACGGAGG TGGTCTTCCT GGCCAGGAAC GACACCATCG TGAACGAGCT GGTACGTGTG 
ACTAGCGAGG GGGCCGGCCT GCAGCTGAGG AAGGTGACCG TCCTGGGGGT GGCCGCGGGC 
CCCAGGCAGG TCCTCTGCAA CGGCGTCCCT GTTGCCTTCA CCTACAACCC TGACACCAAG 
ACCCTGGACA TCCCCGTGTC ACTGACGATG GGAGAACAGT TTCTCATCAG CTGGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005624022.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624079.1 Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe11090
Clone ID Related Accession (Same CDS sequence)	XM_022422643.1 , XM_005624022.2 , XM_005624022.3
Accession Version	XM_005624022.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysosomal alpha-glucosidase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726072.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624022.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC ACCAACGACC GATGATTAAT CAGCTGTGCT CGGGCCTCCA GGACCCAGCC 
CGCGGCCCGC AGGTGCCACC GACCATGGAC GCGAGGTGGC CTCCCAGCTC CCGGCCCCTG 
CTGGGGGTCT GCACCCTCGT CTGCCTGGCC ACCGCTGCCT TCCTGGGCCA CGTCCTGCTC 
CGTGACTCCT TGGTGGCTCC CCAAGAGCAA CGGCGCTTCT CCCAAGGGCC CGAGCAGCTT 
TATCATGCTC ACCAGCAAGG AGCCCGTAAC CCAGGCCCCC AGCCCAGCCC AGGGCACCCC 
GGCAGGCCCC GAGCGGCACC CACACAGTGT GATGTGCCCC CCAACAGCCG TTTCGACTGT 
GCCCCGGACA AGGCCATCAC CCAGCAGCAG TGTGAGGCGC GGGGCTGCTG CTACAGGCCC 
GCGACGCCCT GGCCCCAGCA CCCCCGGATG GGACAGCCCT GGTGCTTCTT CCCACCCAGC 
TACCCGAGTT ACAAGCTGGA GAACCTGACC ACCACGGAGA CGGGCTACAC GGCCGCTCTG 
ACCCGTAGCA CCCCAACCTT CTTCCCCAAG GACATTCTGG CCTTACGGTT GGACGTGCTG 
CTGGAGACGG AGAGCCGGCT CCACTTCACG ATCAAAGATC CCACCAACAG GCGCTATGAG 
GTGCCCCTGG AGACCCCGCG GGCCCACGGC CGCGCCTTGG CCACACTCTA CAGCGTGGAG 
TTCCAGGAGG AGCCCTTCGG GGTGGTCGTG CGCCGGAAGC TGGACGGCCG GGTGCTGCTG 
AACACGACGG TGGCCCCCCT GTTCTTCGCC GACCAGTTTC TGCAGCTGTC CACCTCCCTG 
CCGTCCCAGT ACATCGCTGG CCTTGCCGAG CATCTCGGCT CCCTGATGCT CAGCACCAAC 
TGGACCAGGG TCACCCTCTG GAACCGGGAC ATCGCCCCCT CGCCCAACGT GAACCTGTAC 
GGGTCCCACC CTTTCTACCT GGCGCTGGAG GATGGCGGGT CAGCTCATGG GGTCTTCCTG 
CTGAACAGCA ATGCCATGGA CGTGGTCCTG CAGCCGAGCC CGGCCCTCAG CTGGAGGTCG 
ACAGGCGGGA TCCTGGACGT GTACGTCTTC CTGGGCCCGG AGCCCAAGAG CGTGGTACAG 
CAGTACCTGG AAGTCGTGGG CTCCCCGTTC ATGCCACCCT ACTGGGGCCT GGGCTTCCAC 
CTGTGCCGCT GGGGATACTC GTCCACAGCC ATCACCCGCC AGGTGGTAGA GAACATGACC 
CGGGCCCACT TCCCCCTGGA CACGCAGTGG AACGACCTGG ATTACATGGA CGCCAGGAGG 
GACTTTACTT TCAACAAAGA CGGCTTCAGG GACTTCCCGG CCATGGTGCA GGAGCTCCAC 
CGGGGCGGCC GGCGGTACGT GATGATCGTG GACCCGGCCA TCAGCAGCTC CAGCCCCCCT 
GGCAGCTACC GACCCTATGA CGAGGGTCTG CGGAGAAAGG TTTTCATCAC CAATGAGACG 
GGGCAGCCGC TGATTGGGAA GGTGTGGCCC GGGTTCACTG TCTTCCCCGA CTTCACCAGC 
CCCACGGCCC TGGACTGGTG GCAGGACATG GTGTCTGAGT TCCATGCCCA GGTGCCCTTC 
GACGGCATGT GGATTGACAT GAACGAGCCA TCCAACTTCG TGAGGGGCTC GGTGTATGGC 
TGCCCCGACA ATGACCTGGA GAACCCGCCC TACGTGCCCG GGGTGGTTGG CGGGACCCTG 
CGGGCAGCCA CCATCTGTGC CTCCAGCCGC CAGCTGCTCT CCACGCACTA CAACCTGCAC 
AACCTGTATG GCCTGACGGA AGCCATCGCC TCCCACAGGG CCCTCGTGAA GGCTCGGGGG 
ACGCGCCCCT TTGTGATCTC CCGCTCAACC TTCGCTGGCC ACGGCCAGTA TGCCGGCCAC 
TGGACAGGGG ACGTGTGGAG CAGCTGGGAG CAGCTCTCGT ACTCTGTACC AGAAATCCTG 
CTGTTCAACC TGCTGGGGGT GCCGCTGGTC GGGGCCGACG TCTGTGGCTT CCTGGGCAAC 
ACGTCAGAGG AGCTGTGCGT GCGCTGGACC CAGCTGGGGG CCTTCTATCC GTTCATGCGG 
AACCACAACG ACCTCAACAG CCTGCCCCAG GAGCCCTACA GGTTCAGCGC GACGGCGCAG 
GAAGCCATGA GGAAGGCGCT CGCCCTGCGC TACTCGCTGC TGCCCCACCT CTACACGCTC 
TTCCACCGGG CACACGTGGG GGGCGAGACC GTGGCCCGGC CCCTCTTCCT CGAGTTCCCT 
GAAGACCCCC ACACCTGGAC CGTGGACCGC CAGCTCCTGT GGGGGGAGGC TCTGCTCATC 
ACCCCCGTGC TCGAGGCCGG GAAGGTCGAA GTGACTGGCT ACTTCCCCGC TGGCACATGG 
TACGACCTGC AGACGGTGCC CGTAGGGGCC TTCGGCAGCC TCCCGCCCCC ACCTCCAGCT 
CCGCTCATGT CCACCATCCA CAGCAAGGGG CAGTGGGTGA CGCTGCCAGC CCCGCTGGAC 
ACCATCAACG TCCACCTCCG GGCTGGGCAC ATCATCCCCC TGCAGGGCCC TGGCCTCACG 
ACCACAGAGT CCCGAAAGCA GCCCATGGCC CTGGTCGCAG CCCTGGGCAC AAACGGGGAA 
GCCCGAGGGG AGCTGTTCTG GGACGACGGG GAGAGCCTCG GGGTGCTGGA GCGCGGGGCC 
TACACGGAGG TGGTCTTCCT GGCCAGGAAC GACACCATCG TGAACGAGCT GGTACGTGTG 
ACTAGCGAGG GGGCCGGCCT GCAGCTGAGG AAGGTGACCG TCCTGGGGGT GGCCGCGGGC 
CCCAGGCAGG TCCTCTGCAA CGGCGTCCCT GTTGCCTTCA CCTACAACCC TGACACCAAG 
ACCCTGGACA TCCCCGTGTC ACTGACGATG GGAGAACAGT TTCTCATCAG CTGGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624079.1
CDS	530..3469
Translation	MGHHQRPMINQLCSGLQDPARGPQVPPTMDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_005624022.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624022.2

ORF Insert Sequence:

ATGGGGCATC ACCAACGACC GATGATTAAT CAGCTGTGCT CGGGCCTCCA GGACCCAGCC 
CGCGGCCCGC AGGTGCCACC GACCATGGAC GCGAGGTGGC CTCCCAGCTC CCGGCCCCTG 
CTGGGGGTCT GCACCCTCGT CTGCCTGGCC ACCGCTGCCT TCCTGGGCCA CGTCCTGCTC 
CGTGACTCCT TGGTGGCTCC CCAAGAGCAA CGGCGCTTCT CCCAAGGGCC CGAGCAGCTT 
TATCATGCTC ACCAGCAAGG AGCCCGTAAC CCAGGCCCCC AGCCCAGCCC AGGGCACCCC 
GGCAGGCCCC GAGCGGCACC CACACAGTGT GATGTGCCCC CCAACAGCCG TTTCGACTGT 
GCCCCGGACA AGGCCATCAC CCAGCAGCAG TGTGAGGCGC GGGGCTGCTG CTACAGGCCC 
GCGACGCCCT GGCCCCAGCA CCCCCGGATG GGACAGCCCT GGTGCTTCTT CCCACCCAGC 
TACCCGAGTT ACAAGCTGGA GAACCTGACC ACCACGGAGA CGGGCTACAC GGCCGCTCTG 
ACCCGTAGCA CCCCAACCTT CTTCCCCAAG GACATTCTGG CCTTACGGTT GGACGTGCTG 
CTGGAGACGG AGAGCCGGCT CCACTTCACG ATCAAAGATC CCACCAACAG GCGCTATGAG 
GTGCCCCTGG AGACCCCGCG GGCCCACGGC CGCGCCTTGG CCACACTCTA CAGCGTGGAG 
TTCCAGGAGG AGCCCTTCGG GGTGGTCGTG CGCCGGAAGC TGGACGGCCG GGTGCTGCTG 
AACACGACGG TGGCCCCCCT GTTCTTCGCC GACCAGTTTC TGCAGCTGTC CACCTCCCTG 
CCGTCCCAGT ACATCGCTGG CCTTGCCGAG CATCTCGGCT CCCTGATGCT CAGCACCAAC 
TGGACCAGGG TCACCCTCTG GAACCGGGAC ATCGCCCCCT CGCCCAACGT GAACCTGTAC 
GGGTCCCACC CTTTCTACCT GGCGCTGGAG GATGGCGGGT CAGCTCATGG GGTCTTCCTG 
CTGAACAGCA ATGCCATGGA CGTGGTCCTG CAGCCGAGCC CGGCCCTCAG CTGGAGGTCG 
ACAGGCGGGA TCCTGGACGT GTACGTCTTC CTGGGCCCGG AGCCCAAGAG CGTGGTACAG 
CAGTACCTGG AAGTCGTGGG CTCCCCGTTC ATGCCACCCT ACTGGGGCCT GGGCTTCCAC 
CTGTGCCGCT GGGGATACTC GTCCACAGCC ATCACCCGCC AGGTGGTAGA GAACATGACC 
CGGGCCCACT TCCCCCTGGA CACGCAGTGG AACGACCTGG ATTACATGGA CGCCAGGAGG 
GACTTTACTT TCAACAAAGA CGGCTTCAGG GACTTCCCGG CCATGGTGCA GGAGCTCCAC 
CGGGGCGGCC GGCGGTACGT GATGATCGTG GACCCGGCCA TCAGCAGCTC CAGCCCCCCT 
GGCAGCTACC GACCCTATGA CGAGGGTCTG CGGAGAAAGG TTTTCATCAC CAATGAGACG 
GGGCAGCCGC TGATTGGGAA GGTGTGGCCC GGGTTCACTG TCTTCCCCGA CTTCACCAGC 
CCCACGGCCC TGGACTGGTG GCAGGACATG GTGTCTGAGT TCCATGCCCA GGTGCCCTTC 
GACGGCATGT GGATTGACAT GAACGAGCCA TCCAACTTCG TGAGGGGCTC GGTGTATGGC 
TGCCCCGACA ATGACCTGGA GAACCCGCCC TACGTGCCCG GGGTGGTTGG CGGGACCCTG 
CGGGCAGCCA CCATCTGTGC CTCCAGCCGC CAGCTGCTCT CCACGCACTA CAACCTGCAC 
AACCTGTATG GCCTGACGGA AGCCATCGCC TCCCACAGGG CCCTCGTGAA GGCTCGGGGG 
ACGCGCCCCT TTGTGATCTC CCGCTCAACC TTCGCTGGCC ACGGCCAGTA TGCCGGCCAC 
TGGACAGGGG ACGTGTGGAG CAGCTGGGAG CAGCTCTCGT ACTCTGTACC AGAAATCCTG 
CTGTTCAACC TGCTGGGGGT GCCGCTGGTC GGGGCCGACG TCTGTGGCTT CCTGGGCAAC 
ACGTCAGAGG AGCTGTGCGT GCGCTGGACC CAGCTGGGGG CCTTCTATCC GTTCATGCGG 
AACCACAACG ACCTCAACAG CCTGCCCCAG GAGCCCTACA GGTTCAGCGC GACGGCGCAG 
GAAGCCATGA GGAAGGCGCT CGCCCTGCGC TACTCGCTGC TGCCCCACCT CTACACGCTC 
TTCCACCGGG CACACGTGGG GGGCGAGACC GTGGCCCGGC CCCTCTTCCT CGAGTTCCCT 
GAAGACCCCC ACACCTGGAC CGTGGACCGC CAGCTCCTGT GGGGGGAGGC TCTGCTCATC 
ACCCCCGTGC TCGAGGCCGG GAAGGTCGAA GTGACTGGCT ACTTCCCCGC TGGCACATGG 
TACGACCTGC AGACGGTGCC CGTAGGGGCC TTCGGCAGCC TCCCGCCCCC ACCTCCAGCT 
CCGCTCATGT CCACCATCCA CAGCAAGGGG CAGTGGGTGA CGCTGCCAGC CCCGCTGGAC 
ACCATCAACG TCCACCTCCG GGCTGGGCAC ATCATCCCCC TGCAGGGCCC TGGCCTCACG 
ACCACAGAGT CCCGAAAGCA GCCCATGGCC CTGGTCGCAG CCCTGGGCAC AAACGGGGAA 
GCCCGAGGGG AGCTGTTCTG GGACGACGGG GAGAGCCTCG GGGTGCTGGA GCGCGGGGCC 
TACACGGAGG TGGTCTTCCT GGCCAGGAAC GACACCATCG TGAACGAGCT GGTACGTGTG 
ACTAGCGAGG GGGCCGGCCT GCAGCTGAGG AAGGTGACCG TCCTGGGGGT GGCCGCGGGC 
CCCAGGCAGG TCCTCTGCAA CGGCGTCCCT GTTGCCTTCA CCTACAACCC TGACACCAAG 
ACCCTGGACA TCCCCGTGTC ACTGACGATG GGAGAACAGT TTCTCATCAG CTGGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005624022.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624079.1 Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe11090
Clone ID Related Accession (Same CDS sequence)	XM_022422643.1 , XM_005624022.2 , XM_005624022.3
Accession Version	XM_005624022.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2940bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysosomal alpha-glucosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624022.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC ACCAACGACC GATGATTAAT CAGCTGTGCT CGGGCCTCCA GGACCCAGCC 
CGCGGCCCGC AGGTGCCACC GACCATGGAC GCGAGGTGGC CTCCCAGCTC CCGGCCCCTG 
CTGGGGGTCT GCACCCTCGT CTGCCTGGCC ACCGCTGCCT TCCTGGGCCA CGTCCTGCTC 
CGTGACTCCT TGGTGGCTCC CCAAGAGCAA CGGCGCTTCT CCCAAGGGCC CGAGCAGCTT 
TATCATGCTC ACCAGCAAGG AGCCCGTAAC CCAGGCCCCC AGCCCAGCCC AGGGCACCCC 
GGCAGGCCCC GAGCGGCACC CACACAGTGT GATGTGCCCC CCAACAGCCG TTTCGACTGT 
GCCCCGGACA AGGCCATCAC CCAGCAGCAG TGTGAGGCGC GGGGCTGCTG CTACAGGCCC 
GCGACGCCCT GGCCCCAGCA CCCCCGGATG GGACAGCCCT GGTGCTTCTT CCCACCCAGC 
TACCCGAGTT ACAAGCTGGA GAACCTGACC ACCACGGAGA CGGGCTACAC GGCCGCTCTG 
ACCCGTAGCA CCCCAACCTT CTTCCCCAAG GACATTCTGG CCTTACGGTT GGACGTGCTG 
CTGGAGACGG AGAGCCGGCT CCACTTCACG ATCAAAGATC CCACCAACAG GCGCTATGAG 
GTGCCCCTGG AGACCCCGCG GGCCCACGGC CGCGCCTTGG CCACACTCTA CAGCGTGGAG 
TTCCAGGAGG AGCCCTTCGG GGTGGTCGTG CGCCGGAAGC TGGACGGCCG GGTGCTGCTG 
AACACGACGG TGGCCCCCCT GTTCTTCGCC GACCAGTTTC TGCAGCTGTC CACCTCCCTG 
CCGTCCCAGT ACATCGCTGG CCTTGCCGAG CATCTCGGCT CCCTGATGCT CAGCACCAAC 
TGGACCAGGG TCACCCTCTG GAACCGGGAC ATCGCCCCCT CGCCCAACGT GAACCTGTAC 
GGGTCCCACC CTTTCTACCT GGCGCTGGAG GATGGCGGGT CAGCTCATGG GGTCTTCCTG 
CTGAACAGCA ATGCCATGGA CGTGGTCCTG CAGCCGAGCC CGGCCCTCAG CTGGAGGTCG 
ACAGGCGGGA TCCTGGACGT GTACGTCTTC CTGGGCCCGG AGCCCAAGAG CGTGGTACAG 
CAGTACCTGG AAGTCGTGGG CTCCCCGTTC ATGCCACCCT ACTGGGGCCT GGGCTTCCAC 
CTGTGCCGCT GGGGATACTC GTCCACAGCC ATCACCCGCC AGGTGGTAGA GAACATGACC 
CGGGCCCACT TCCCCCTGGA CACGCAGTGG AACGACCTGG ATTACATGGA CGCCAGGAGG 
GACTTTACTT TCAACAAAGA CGGCTTCAGG GACTTCCCGG CCATGGTGCA GGAGCTCCAC 
CGGGGCGGCC GGCGGTACGT GATGATCGTG GACCCGGCCA TCAGCAGCTC CAGCCCCCCT 
GGCAGCTACC GACCCTATGA CGAGGGTCTG CGGAGAAAGG TTTTCATCAC CAATGAGACG 
GGGCAGCCGC TGATTGGGAA GGTGTGGCCC GGGTTCACTG TCTTCCCCGA CTTCACCAGC 
CCCACGGCCC TGGACTGGTG GCAGGACATG GTGTCTGAGT TCCATGCCCA GGTGCCCTTC 
GACGGCATGT GGATTGACAT GAACGAGCCA TCCAACTTCG TGAGGGGCTC GGTGTATGGC 
TGCCCCGACA ATGACCTGGA GAACCCGCCC TACGTGCCCG GGGTGGTTGG CGGGACCCTG 
CGGGCAGCCA CCATCTGTGC CTCCAGCCGC CAGCTGCTCT CCACGCACTA CAACCTGCAC 
AACCTGTATG GCCTGACGGA AGCCATCGCC TCCCACAGGG CCCTCGTGAA GGCTCGGGGG 
ACGCGCCCCT TTGTGATCTC CCGCTCAACC TTCGCTGGCC ACGGCCAGTA TGCCGGCCAC 
TGGACAGGGG ACGTGTGGAG CAGCTGGGAG CAGCTCTCGT ACTCTGTACC AGAAATCCTG 
CTGTTCAACC TGCTGGGGGT GCCGCTGGTC GGGGCCGACG TCTGTGGCTT CCTGGGCAAC 
ACGTCAGAGG AGCTGTGCGT GCGCTGGACC CAGCTGGGGG CCTTCTATCC GTTCATGCGG 
AACCACAACG ACCTCAACAG CCTGCCCCAG GAGCCCTACA GGTTCAGCGC GACGGCGCAG 
GAAGCCATGA GGAAGGCGCT CGCCCTGCGC TACTCGCTGC TGCCCCACCT CTACACGCTC 
TTCCACCGGG CACACGTGGG GGGCGAGACC GTGGCCCGGC CCCTCTTCCT CGAGTTCCCT 
GAAGACCCCC ACACCTGGAC CGTGGACCGC CAGCTCCTGT GGGGGGAGGC TCTGCTCATC 
ACCCCCGTGC TCGAGGCCGG GAAGGTCGAA GTGACTGGCT ACTTCCCCGC TGGCACATGG 
TACGACCTGC AGACGGTGCC CGTAGGGGCC TTCGGCAGCC TCCCGCCCCC ACCTCCAGCT 
CCGCTCATGT CCACCATCCA CAGCAAGGGG CAGTGGGTGA CGCTGCCAGC CCCGCTGGAC 
ACCATCAACG TCCACCTCCG GGCTGGGCAC ATCATCCCCC TGCAGGGCCC TGGCCTCACG 
ACCACAGAGT CCCGAAAGCA GCCCATGGCC CTGGTCGCAG CCCTGGGCAC AAACGGGGAA 
GCCCGAGGGG AGCTGTTCTG GGACGACGGG GAGAGCCTCG GGGTGCTGGA GCGCGGGGCC 
TACACGGAGG TGGTCTTCCT GGCCAGGAAC GACACCATCG TGAACGAGCT GGTACGTGTG 
ACTAGCGAGG GGGCCGGCCT GCAGCTGAGG AAGGTGACCG TCCTGGGGGT GGCCGCGGGC 
CCCAGGCAGG TCCTCTGCAA CGGCGTCCCT GTTGCCTTCA CCTACAACCC TGACACCAAG 
ACCCTGGACA TCCCCGTGTC ACTGACGATG GGAGAACAGT TTCTCATCAG CTGGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624079.1
CDS	324..3263
Translation	MGHHQRPMINQLCSGLQDPARGPQVPPTMDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_005624022.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624022.3

ORF Insert Sequence:

ATGGGGCATC ACCAACGACC GATGATTAAT CAGCTGTGCT CGGGCCTCCA GGACCCAGCC 
CGCGGCCCGC AGGTGCCACC GACCATGGAC GCGAGGTGGC CTCCCAGCTC CCGGCCCCTG 
CTGGGGGTCT GCACCCTCGT CTGCCTGGCC ACCGCTGCCT TCCTGGGCCA CGTCCTGCTC 
CGTGACTCCT TGGTGGCTCC CCAAGAGCAA CGGCGCTTCT CCCAAGGGCC CGAGCAGCTT 
TATCATGCTC ACCAGCAAGG AGCCCGTAAC CCAGGCCCCC AGCCCAGCCC AGGGCACCCC 
GGCAGGCCCC GAGCGGCACC CACACAGTGT GATGTGCCCC CCAACAGCCG TTTCGACTGT 
GCCCCGGACA AGGCCATCAC CCAGCAGCAG TGTGAGGCGC GGGGCTGCTG CTACAGGCCC 
GCGACGCCCT GGCCCCAGCA CCCCCGGATG GGACAGCCCT GGTGCTTCTT CCCACCCAGC 
TACCCGAGTT ACAAGCTGGA GAACCTGACC ACCACGGAGA CGGGCTACAC GGCCGCTCTG 
ACCCGTAGCA CCCCAACCTT CTTCCCCAAG GACATTCTGG CCTTACGGTT GGACGTGCTG 
CTGGAGACGG AGAGCCGGCT CCACTTCACG ATCAAAGATC CCACCAACAG GCGCTATGAG 
GTGCCCCTGG AGACCCCGCG GGCCCACGGC CGCGCCTTGG CCACACTCTA CAGCGTGGAG 
TTCCAGGAGG AGCCCTTCGG GGTGGTCGTG CGCCGGAAGC TGGACGGCCG GGTGCTGCTG 
AACACGACGG TGGCCCCCCT GTTCTTCGCC GACCAGTTTC TGCAGCTGTC CACCTCCCTG 
CCGTCCCAGT ACATCGCTGG CCTTGCCGAG CATCTCGGCT CCCTGATGCT CAGCACCAAC 
TGGACCAGGG TCACCCTCTG GAACCGGGAC ATCGCCCCCT CGCCCAACGT GAACCTGTAC 
GGGTCCCACC CTTTCTACCT GGCGCTGGAG GATGGCGGGT CAGCTCATGG GGTCTTCCTG 
CTGAACAGCA ATGCCATGGA CGTGGTCCTG CAGCCGAGCC CGGCCCTCAG CTGGAGGTCG 
ACAGGCGGGA TCCTGGACGT GTACGTCTTC CTGGGCCCGG AGCCCAAGAG CGTGGTACAG 
CAGTACCTGG AAGTCGTGGG CTCCCCGTTC ATGCCACCCT ACTGGGGCCT GGGCTTCCAC 
CTGTGCCGCT GGGGATACTC GTCCACAGCC ATCACCCGCC AGGTGGTAGA GAACATGACC 
CGGGCCCACT TCCCCCTGGA CACGCAGTGG AACGACCTGG ATTACATGGA CGCCAGGAGG 
GACTTTACTT TCAACAAAGA CGGCTTCAGG GACTTCCCGG CCATGGTGCA GGAGCTCCAC 
CGGGGCGGCC GGCGGTACGT GATGATCGTG GACCCGGCCA TCAGCAGCTC CAGCCCCCCT 
GGCAGCTACC GACCCTATGA CGAGGGTCTG CGGAGAAAGG TTTTCATCAC CAATGAGACG 
GGGCAGCCGC TGATTGGGAA GGTGTGGCCC GGGTTCACTG TCTTCCCCGA CTTCACCAGC 
CCCACGGCCC TGGACTGGTG GCAGGACATG GTGTCTGAGT TCCATGCCCA GGTGCCCTTC 
GACGGCATGT GGATTGACAT GAACGAGCCA TCCAACTTCG TGAGGGGCTC GGTGTATGGC 
TGCCCCGACA ATGACCTGGA GAACCCGCCC TACGTGCCCG GGGTGGTTGG CGGGACCCTG 
CGGGCAGCCA CCATCTGTGC CTCCAGCCGC CAGCTGCTCT CCACGCACTA CAACCTGCAC 
AACCTGTATG GCCTGACGGA AGCCATCGCC TCCCACAGGG CCCTCGTGAA GGCTCGGGGG 
ACGCGCCCCT TTGTGATCTC CCGCTCAACC TTCGCTGGCC ACGGCCAGTA TGCCGGCCAC 
TGGACAGGGG ACGTGTGGAG CAGCTGGGAG CAGCTCTCGT ACTCTGTACC AGAAATCCTG 
CTGTTCAACC TGCTGGGGGT GCCGCTGGTC GGGGCCGACG TCTGTGGCTT CCTGGGCAAC 
ACGTCAGAGG AGCTGTGCGT GCGCTGGACC CAGCTGGGGG CCTTCTATCC GTTCATGCGG 
AACCACAACG ACCTCAACAG CCTGCCCCAG GAGCCCTACA GGTTCAGCGC GACGGCGCAG 
GAAGCCATGA GGAAGGCGCT CGCCCTGCGC TACTCGCTGC TGCCCCACCT CTACACGCTC 
TTCCACCGGG CACACGTGGG GGGCGAGACC GTGGCCCGGC CCCTCTTCCT CGAGTTCCCT 
GAAGACCCCC ACACCTGGAC CGTGGACCGC CAGCTCCTGT GGGGGGAGGC TCTGCTCATC 
ACCCCCGTGC TCGAGGCCGG GAAGGTCGAA GTGACTGGCT ACTTCCCCGC TGGCACATGG 
TACGACCTGC AGACGGTGCC CGTAGGGGCC TTCGGCAGCC TCCCGCCCCC ACCTCCAGCT 
CCGCTCATGT CCACCATCCA CAGCAAGGGG CAGTGGGTGA CGCTGCCAGC CCCGCTGGAC 
ACCATCAACG TCCACCTCCG GGCTGGGCAC ATCATCCCCC TGCAGGGCCC TGGCCTCACG 
ACCACAGAGT CCCGAAAGCA GCCCATGGCC CTGGTCGCAG CCCTGGGCAC AAACGGGGAA 
GCCCGAGGGG AGCTGTTCTG GGACGACGGG GAGAGCCTCG GGGTGCTGGA GCGCGGGGCC 
TACACGGAGG TGGTCTTCCT GGCCAGGAAC GACACCATCG TGAACGAGCT GGTACGTGTG 
ACTAGCGAGG GGGCCGGCCT GCAGCTGAGG AAGGTGACCG TCCTGGGGGT GGCCGCGGGC 
CCCAGGCAGG TCCTCTGCAA CGGCGTCCCT GTTGCCTTCA CCTACAACCC TGACACCAAG 
ACCCTGGACA TCCCCGTGTC ACTGACGATG GGAGAACAGT TTCTCATCAG CTGGTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005624023.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624080.1 Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe11091
Clone ID Related Accession (Same CDS sequence)	XM_005624023.2 , XM_845556.5 , XM_005624023.3 , XM_845556.4
Accession Version	XM_005624023.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysosomal alpha-glucosidase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726072.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005624023.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624080.1
CDS	134..2989
Translation	MDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_005624023.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624023.2

ORF Insert Sequence:

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_845556.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850649.1 Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe11091
Clone ID Related Accession (Same CDS sequence)	XM_005624023.2 , XM_845556.5 , XM_005624023.3 , XM_845556.4
Accession Version	XM_845556.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysosomal alpha-glucosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_845556.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850649.1
CDS	93..2948
Translation	MDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_845556.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_845556.5

ORF Insert Sequence:

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_005624023.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005624080.1 Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe11091
Clone ID Related Accession (Same CDS sequence)	XM_005624023.2 , XM_845556.5 , XM_005624023.3 , XM_845556.4
Accession Version	XM_005624023.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	lysosomal alpha-glucosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005624023.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005624080.1
CDS	61..2916
Translation	MDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_005624023.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosidase alpha, acid (GAA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005624023.3

ORF Insert Sequence:

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAA ( XM_845556.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_850649.1 Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.

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CloneID	OCe11091
Clone ID Related Accession (Same CDS sequence)	XM_005624023.2 , XM_845556.5 , XM_005624023.3 , XM_845556.4
Accession Version	XM_845556.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	lysosomal alpha-glucosidase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726072.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_845556.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_850649.1
CDS	99..2954
Translation	MDARWPPSSRPLLGVCTLVCLATAAFLGHVLLRDSLVAPQEQRRFSQGPEQLYHAHQQGARNPGPQPSPGHPGRPRAAPTQCDVPPNSRFDCAPDKAITQQQCEARGCCYRPATPWPQHPRMGQPWCFFPPSYPSYKLENLTTTETGYTAALTRSTPTFFPKDILALRLDVLLETESRLHFTIKDPTNRRYEVPLETPRAHGRALATLYSVEFQEEPFGVVVRRKLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYIAGLAEHLGSLMLSTNWTRVTLWNRDIAPSPNVNLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYVFLGPEPKSVVQQYLEVVGSPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDTQWNDLDYMDARRDFTFNKDGFRDFPAMVQELHRGGRRYVMIVDPAISSSSPPGSYRPYDEGLRRKVFITNETGQPLIGKVWPGFTVFPDFTSPTALDWWQDMVSEFHAQVPFDGMWIDMNEPSNFVRGSVYGCPDNDLENPPYVPGVVGGTLRAATICASSRQLLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGQYAGHWTGDVWSSWEQLSYSVPEILLFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNDLNSLPQEPYRFSATAQEAMRKALALRYSLLPHLYTLFHRAHVGGETVARPLFLEFPEDPHTWTVDRQLLWGEALLITPVLEAGKVEVTGYFPAGTWYDLQTVPVGAFGSLPPPPPAPLMSTIHSKGQWVTLPAPLDTINVHLRAGHIIPLQGPGLTTTESRKQPMALVAALGTNGEARGELFWDDGESLGVLERGAYTEVVFLARNDTIVNELVRVTSEGAGLQLRKVTVLGVAAGPRQVLCNGVPVAFTYNPDTKTLDIPVSLTMGEQFLISWS

Target ORF information:

RefSeq Version	XM_845556.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucosidase, alpha; acid (GAA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_845556.4

ORF Insert Sequence:

ATGGACGCGA GGTGGCCTCC CAGCTCCCGG CCCCTGCTGG GGGTCTGCAC CCTCGTCTGC 
CTGGCCACCG CTGCCTTCCT GGGCCACGTC CTGCTCCGTG ACTCCTTGGT GGCTCCCCAA 
GAGCAACGGC GCTTCTCCCA AGGGCCCGAG CAGCTTTATC ATGCTCACCA GCAAGGAGCC 
CGTAACCCAG GCCCCCAGCC CAGCCCAGGG CACCCCGGCA GGCCCCGAGC GGCACCCACA 
CAGTGTGATG TGCCCCCCAA CAGCCGTTTC GACTGTGCCC CGGACAAGGC CATCACCCAG 
CAGCAGTGTG AGGCGCGGGG CTGCTGCTAC AGGCCCGCGA CGCCCTGGCC CCAGCACCCC 
CGGATGGGAC AGCCCTGGTG CTTCTTCCCA CCCAGCTACC CGAGTTACAA GCTGGAGAAC 
CTGACCACCA CGGAGACGGG CTACACGGCC GCTCTGACCC GTAGCACCCC AACCTTCTTC 
CCCAAGGACA TTCTGGCCTT ACGGTTGGAC GTGCTGCTGG AGACGGAGAG CCGGCTCCAC 
TTCACGATCA AAGATCCCAC CAACAGGCGC TATGAGGTGC CCCTGGAGAC CCCGCGGGCC 
CACGGCCGCG CCTTGGCCAC ACTCTACAGC GTGGAGTTCC AGGAGGAGCC CTTCGGGGTG 
GTCGTGCGCC GGAAGCTGGA CGGCCGGGTG CTGCTGAACA CGACGGTGGC CCCCCTGTTC 
TTCGCCGACC AGTTTCTGCA GCTGTCCACC TCCCTGCCGT CCCAGTACAT CGCTGGCCTT 
GCCGAGCATC TCGGCTCCCT GATGCTCAGC ACCAACTGGA CCAGGGTCAC CCTCTGGAAC 
CGGGACATCG CCCCCTCGCC CAACGTGAAC CTGTACGGGT CCCACCCTTT CTACCTGGCG 
CTGGAGGATG GCGGGTCAGC TCATGGGGTC TTCCTGCTGA ACAGCAATGC CATGGACGTG 
GTCCTGCAGC CGAGCCCGGC CCTCAGCTGG AGGTCGACAG GCGGGATCCT GGACGTGTAC 
GTCTTCCTGG GCCCGGAGCC CAAGAGCGTG GTACAGCAGT ACCTGGAAGT CGTGGGCTCC 
CCGTTCATGC CACCCTACTG GGGCCTGGGC TTCCACCTGT GCCGCTGGGG ATACTCGTCC 
ACAGCCATCA CCCGCCAGGT GGTAGAGAAC ATGACCCGGG CCCACTTCCC CCTGGACACG 
CAGTGGAACG ACCTGGATTA CATGGACGCC AGGAGGGACT TTACTTTCAA CAAAGACGGC 
TTCAGGGACT TCCCGGCCAT GGTGCAGGAG CTCCACCGGG GCGGCCGGCG GTACGTGATG 
ATCGTGGACC CGGCCATCAG CAGCTCCAGC CCCCCTGGCA GCTACCGACC CTATGACGAG 
GGTCTGCGGA GAAAGGTTTT CATCACCAAT GAGACGGGGC AGCCGCTGAT TGGGAAGGTG 
TGGCCCGGGT TCACTGTCTT CCCCGACTTC ACCAGCCCCA CGGCCCTGGA CTGGTGGCAG 
GACATGGTGT CTGAGTTCCA TGCCCAGGTG CCCTTCGACG GCATGTGGAT TGACATGAAC 
GAGCCATCCA ACTTCGTGAG GGGCTCGGTG TATGGCTGCC CCGACAATGA CCTGGAGAAC 
CCGCCCTACG TGCCCGGGGT GGTTGGCGGG ACCCTGCGGG CAGCCACCAT CTGTGCCTCC 
AGCCGCCAGC TGCTCTCCAC GCACTACAAC CTGCACAACC TGTATGGCCT GACGGAAGCC 
ATCGCCTCCC ACAGGGCCCT CGTGAAGGCT CGGGGGACGC GCCCCTTTGT GATCTCCCGC 
TCAACCTTCG CTGGCCACGG CCAGTATGCC GGCCACTGGA CAGGGGACGT GTGGAGCAGC 
TGGGAGCAGC TCTCGTACTC TGTACCAGAA ATCCTGCTGT TCAACCTGCT GGGGGTGCCG 
CTGGTCGGGG CCGACGTCTG TGGCTTCCTG GGCAACACGT CAGAGGAGCT GTGCGTGCGC 
TGGACCCAGC TGGGGGCCTT CTATCCGTTC ATGCGGAACC ACAACGACCT CAACAGCCTG 
CCCCAGGAGC CCTACAGGTT CAGCGCGACG GCGCAGGAAG CCATGAGGAA GGCGCTCGCC 
CTGCGCTACT CGCTGCTGCC CCACCTCTAC ACGCTCTTCC ACCGGGCACA CGTGGGGGGC 
GAGACCGTGG CCCGGCCCCT CTTCCTCGAG TTCCCTGAAG ACCCCCACAC CTGGACCGTG 
GACCGCCAGC TCCTGTGGGG GGAGGCTCTG CTCATCACCC CCGTGCTCGA GGCCGGGAAG 
GTCGAAGTGA CTGGCTACTT CCCCGCTGGC ACATGGTACG ACCTGCAGAC GGTGCCCGTA 
GGGGCCTTCG GCAGCCTCCC GCCCCCACCT CCAGCTCCGC TCATGTCCAC CATCCACAGC 
AAGGGGCAGT GGGTGACGCT GCCAGCCCCG CTGGACACCA TCAACGTCCA CCTCCGGGCT 
GGGCACATCA TCCCCCTGCA GGGCCCTGGC CTCACGACCA CAGAGTCCCG AAAGCAGCCC 
ATGGCCCTGG TCGCAGCCCT GGGCACAAAC GGGGAAGCCC GAGGGGAGCT GTTCTGGGAC 
GACGGGGAGA GCCTCGGGGT GCTGGAGCGC GGGGCCTACA CGGAGGTGGT CTTCCTGGCC 
AGGAACGACA CCATCGTGAA CGAGCTGGTA CGTGTGACTA GCGAGGGGGC CGGCCTGCAG 
CTGAGGAAGG TGACCGTCCT GGGGGTGGCC GCGGGCCCCA GGCAGGTCCT CTGCAACGGC 
GTCCCTGTTG CCTTCACCTA CAACCCTGAC ACCAAGACCC TGGACATCCC CGTGTCACTG 
ACGATGGGAG AACAGTTTCT CATCAGCTGG TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GAA cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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