NOTCH2 cDNA ORF clone, Canis lupus familiaris(dog)

The following NOTCH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOTCH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe19354 XM_005630688.3
Latest version!
Canis lupus familiaris notch 2 (NOTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe294489 XM_022404895.1
Latest version!
Canis lupus familiaris notch 2 (NOTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe294490 XM_022404896.1
Latest version!
Canis lupus familiaris notch 2 (NOTCH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
OCe40889 XM_014120603.2
Latest version!
Canis lupus familiaris notch 2 (NOTCH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
OCe19354 XM_005630688.2 Canis lupus familiaris notch 2 (NOTCH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
OCe40889 XM_014120603.1 Canis lupus familiaris notch 2 (NOTCH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe19354
    Clone ID Related Accession (Same CDS sequence) XM_005630688.2 , XM_005630688.3
    Accession Version XM_005630688.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurogenic locus notch homolog protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005630688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGAAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTCCTGGATG AGTACAATGT GACGCCAAGC CCTCCAGGCA CTGTGCTGAC TTCTGCACTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TAAAGCATAC CCCCATGGGC
    AAGAAGCCTA GACGGCCCAA TACCAAGAGT ACCATGCCTA CCAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA CAAGGGTCAG
    CTGTCTGAGA GCTCAGTGAC CTTATCCCCC GTTGATTCCT TAGAGTCTCC TCACACATAT
    GTTTCTGACA CCACATCCTC TCCAATGATC ACATCCCCTG GAATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACCGC TGCCCCCCCT GCCCCAGCCC ATGCTCAGCA TGCACTGTCC
    TTCTCTAACC TGCATGAAAT GCAGCCGTTG GCTCATGGTG CCAGCACTGT GCTTCCCTCA
    GTTAGCCAGC TGCTGTCCCA CCACCACATT GCTCCCCCAG GCAACGGCAG TGCAGGGAGC
    TTGGGTAGGC TCCATCCTGT CACTGTTTCG GCAGATTGGA TGAACCGCAT GGAGATGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTTCTGGCTC CAGCTGAGAG CACGCATCCT
    GGCATACCTC CCCAGAGCAG GCCGCCTGAA GGGAAGCACA TAACTACCCC TCGGGAACCT
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GTATTGCCCA ACCAGCAGGG
    ACTCCCCAGC CTCAGTCTAA CTGCCCTCCA CCTGTTGCAG GCCCTATGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG TTTGCCCAGT ATGGCTTTCC CCACTGCCAT GGTGCCGCAG
    CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ATCCTTTTCC GGCATCTGTG
    GGCAAGTATC CCACACCACC TTCACAGCAT AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCTAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGAAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTTTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630745.1
    CDS66..7481
    Translation

    Target ORF information:

    RefSeq Version XM_005630688.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris notch 2 (NOTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630688.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGAAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTCCTGGATG AGTACAATGT GACGCCAAGC CCTCCAGGCA CTGTGCTGAC TTCTGCACTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TAAAGCATAC CCCCATGGGC
    AAGAAGCCTA GACGGCCCAA TACCAAGAGT ACCATGCCTA CCAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA CAAGGGTCAG
    CTGTCTGAGA GCTCAGTGAC CTTATCCCCC GTTGATTCCT TAGAGTCTCC TCACACATAT
    GTTTCTGACA CCACATCCTC TCCAATGATC ACATCCCCTG GAATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACCGC TGCCCCCCCT GCCCCAGCCC ATGCTCAGCA TGCACTGTCC
    TTCTCTAACC TGCATGAAAT GCAGCCGTTG GCTCATGGTG CCAGCACTGT GCTTCCCTCA
    GTTAGCCAGC TGCTGTCCCA CCACCACATT GCTCCCCCAG GCAACGGCAG TGCAGGGAGC
    TTGGGTAGGC TCCATCCTGT CACTGTTTCG GCAGATTGGA TGAACCGCAT GGAGATGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTTCTGGCTC CAGCTGAGAG CACGCATCCT
    GGCATACCTC CCCAGAGCAG GCCGCCTGAA GGGAAGCACA TAACTACCCC TCGGGAACCT
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GTATTGCCCA ACCAGCAGGG
    ACTCCCCAGC CTCAGTCTAA CTGCCCTCCA CCTGTTGCAG GCCCTATGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG TTTGCCCAGT ATGGCTTTCC CCACTGCCAT GGTGCCGCAG
    CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ATCCTTTTCC GGCATCTGTG
    GGCAAGTATC CCACACCACC TTCACAGCAT AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCTAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGAAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTTTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe19354
    Clone ID Related Accession (Same CDS sequence) XM_005630688.2 , XM_005630688.3
    Accession Version XM_005630688.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurogenic locus notch homolog protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005630688.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGAAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTCCTGGATG AGTACAATGT GACGCCAAGC CCTCCAGGCA CTGTGCTGAC TTCTGCACTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TAAAGCATAC CCCCATGGGC
    AAGAAGCCTA GACGGCCCAA TACCAAGAGT ACCATGCCTA CCAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA CAAGGGTCAG
    CTGTCTGAGA GCTCAGTGAC CTTATCCCCC GTTGATTCCT TAGAGTCTCC TCACACATAT
    GTTTCTGACA CCACATCCTC TCCAATGATC ACATCCCCTG GAATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACCGC TGCCCCCCCT GCCCCAGCCC ATGCTCAGCA TGCACTGTCC
    TTCTCTAACC TGCATGAAAT GCAGCCGTTG GCTCATGGTG CCAGCACTGT GCTTCCCTCA
    GTTAGCCAGC TGCTGTCCCA CCACCACATT GCTCCCCCAG GCAACGGCAG TGCAGGGAGC
    TTGGGTAGGC TCCATCCTGT CACTGTTTCG GCAGATTGGA TGAACCGCAT GGAGATGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTTCTGGCTC CAGCTGAGAG CACGCATCCT
    GGCATACCTC CCCAGAGCAG GCCGCCTGAA GGGAAGCACA TAACTACCCC TCGGGAACCT
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GTATTGCCCA ACCAGCAGGG
    ACTCCCCAGC CTCAGTCTAA CTGCCCTCCA CCTGTTGCAG GCCCTATGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG TTTGCCCAGT ATGGCTTTCC CCACTGCCAT GGTGCCGCAG
    CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ATCCTTTTCC GGCATCTGTG
    GGCAAGTATC CCACACCACC TTCACAGCAT AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCTAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGAAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTTTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005630745.1
    CDS66..7481
    Translation

    Target ORF information:

    RefSeq Version XM_005630688.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris notch 2 (NOTCH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005630688.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGGAA GAGACACCTC TGTTTCTTGC TGCCCGGGAG
    GGAAGCTATG AAGCAGCCAA GATCCTGTTA GACCATTTTG CCAATCGGGA CATCACAGAC
    CATATGGATC GTCTTCCCCG GGATGTGGCT CGGGACCGCA TGCACCATGA CATCGTGCGC
    CTCCTGGATG AGTACAATGT GACGCCAAGC CCTCCAGGCA CTGTGCTGAC TTCTGCACTC
    TCACCTGTCA TCTGTGGGCC CAACAGATCT TTCCTCAGCC TAAAGCATAC CCCCATGGGC
    AAGAAGCCTA GACGGCCCAA TACCAAGAGT ACCATGCCTA CCAGCCTCCC TAACCTTGCC
    AAGGAGGCCA AGGATGCCAA GGGTAGTAGG AGGAAGAAGT CTCTGAGTGA CAAGGGTCAG
    CTGTCTGAGA GCTCAGTGAC CTTATCCCCC GTTGATTCCT TAGAGTCTCC TCACACATAT
    GTTTCTGACA CCACATCCTC TCCAATGATC ACATCCCCTG GAATCTTACA GGCCTCACCC
    AACCCTATGT TGGCCACCGC TGCCCCCCCT GCCCCAGCCC ATGCTCAGCA TGCACTGTCC
    TTCTCTAACC TGCATGAAAT GCAGCCGTTG GCTCATGGTG CCAGCACTGT GCTTCCCTCA
    GTTAGCCAGC TGCTGTCCCA CCACCACATT GCTCCCCCAG GCAACGGCAG TGCAGGGAGC
    TTGGGTAGGC TCCATCCTGT CACTGTTTCG GCAGATTGGA TGAACCGCAT GGAGATGAAT
    GAGACCCAGT ACAATGAGAT GTTTGGTATG GTTCTGGCTC CAGCTGAGAG CACGCATCCT
    GGCATACCTC CCCAGAGCAG GCCGCCTGAA GGGAAGCACA TAACTACCCC TCGGGAACCT
    TTGCCCCCCA TTGTGACTTT CCAGCTAATC CCCAAAGGCA GTATTGCCCA ACCAGCAGGG
    ACTCCCCAGC CTCAGTCTAA CTGCCCTCCA CCTGTTGCAG GCCCTATGCC CACCATGTAT
    CAGATTCCAG AAATGGCTCG TTTGCCCAGT ATGGCTTTCC CCACTGCCAT GGTGCCGCAG
    CAGGATGGGC AGGTTGCTCA GACCATTCTC CCAGCCTATC ATCCTTTTCC GGCATCTGTG
    GGCAAGTATC CCACACCACC TTCACAGCAT AGTTATGCTT CCTCAAATGC TGCTGAGCGA
    ACACCTAGTC ACAGTGGTCA CCTCCAGGGT GAGCATCCCT ACCTGACACC ATCCCCAGAG
    TCTCCTGACC AGTGGTCAAG TTCATCACCC CACTCTGCTT CTGACTGGTC AGATGTGACC
    ACCAGCCCTA CCCCTGGGGG TGCTGGAGGA GGTCAGCGGG GACCTGGAAC ACACATGTCT
    GAGCCACCAC GCAGCAACAT GCAGGTTTAT GCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294489
    Clone ID Related Accession (Same CDS sequence) XM_022404895.1
    Accession Version XM_022404895.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurogenic locus notch homolog protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGGG GTGCTCCTCA TGGCCTCTGG CTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260603.1
    CDS66..6293
    Translation

    Target ORF information:

    RefSeq Version XM_022404895.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris notch 2 (NOTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404895.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGGG GTGCTCCTCA TGGCCTCTGG CTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294490
    Clone ID Related Accession (Same CDS sequence) XM_022404896.1
    Accession Version XM_022404896.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurogenic locus notch homolog protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGAC TATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260604.1
    CDS66..6272
    Translation

    Target ORF information:

    RefSeq Version XM_022404896.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris notch 2 (NOTCH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404896.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGAC TATTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40889
    Clone ID Related Accession (Same CDS sequence) XM_014120603.1 , XM_014120603.2
    Accession Version XM_014120603.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product neurogenic locus notch homolog protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGTT GCGAATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976078.1
    CDS66..6275
    Translation

    Target ORF information:

    RefSeq Version XM_014120603.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris notch 2 (NOTCH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGTT GCGAATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40889
    Clone ID Related Accession (Same CDS sequence) XM_014120603.1 , XM_014120603.2
    Accession Version XM_014120603.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product neurogenic locus notch homolog protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014120603.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGTT GCGAATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013976078.1
    CDS66..6275
    Translation

    Target ORF information:

    RefSeq Version XM_014120603.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris notch 2 (NOTCH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120603.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCCCGGCC TGAGCCCCGC TCGGCTGTGG GCGCTGCTGG CGCTCGGGCT GAGCTGCGCG 
    GCCCCCGCGC GCGCATTGCA GTGTCGGGAT AGCTATGAGC CCTGTGTAAA TGAAGGAGTA
    TGTGTTACCT ACCACAATGG CACAGGATAC TGCAAATGTC CAGAGGGTTT CTTGGGAGAA
    TATTGTCAAC ATCGAGACCC CTGTGAGAGG AACCGCTGCC AGAATGGTGG GACGTGTGTG
    GCCCAGGCCA TGTTGGGGAA AGCAACATGC CGATGTGCCC TGGGGTTCAC AGGTGATGAT
    TGCCAGTACT CAACCACTCA TCCCTGCTTT GTTTCTCACC CCTGCTTGAA TGGAGGCACC
    TGCCACATGC ATAGCCGGGA TGACTATGAG TGCACCTGCC AAGTTGGTTT TACAGGTAAG
    CTGTGCCAGT GGACTGATGC CTGCCTGTCT CATCCCTGTG CGAATGGAAG TACCTGTACC
    ACTGTGGCCA ACCAGTTCTC CTGCAAATGC CTCACAGGCT TCACCGGACA GAAGTGTGAG
    ATTGATGTCA ATGAGTGTGA CATTCCAGGA CAGTGCCAGC ATGGTGGCAC CTGCCTCAAT
    CTACCGGGTT CCTATCAGTG CCAGTGCCCC CAGGGCTTCA CAGGCAAGCA CTGTGACAGC
    CCCTATGTGC CCTGTGCACC CTCCCCCTGT GTTAATGGTG GCACCTGCCG GCAGACTGGT
    GACTTCACAT TTGAATGCAA CTGCCTTCCA GGTTTTGAAG GGATCACCTG TGAGCGGAAT
    ATTGATGACT GCCCTAACCA CAAGTGTCAG AATGGAGGGG TTTGTGTGGA TGGGGTTAAT
    ACTTACAACT GCCGCTGTCC CCCTCAGTGG ACAGGACAGT TCTGCACAGA GGATGTGGAT
    GAGTGTCTGC TACAACCTAA TGCTTGTCAG AATGGAGGCA CCTGTACCAA CCGCAATGGG
    GGTTATGGCT GTGTATGTGT TAATGGCTGG AGTGGAGATG ACTGCAGTGA GAACATTGAT
    GACTGTGCCT TTGCCTCCTG CACCCCGGGC TCCACCTGCA TTGACCGTGT GGCCTCCTTC
    TCTTGCATGT GCCCAGAAGG AAAGGCAGGT CTATTATGTC ATCTGGATGA TGCATGCATC
    AGCAATCCTT GCCACAAGGG GGCACTGTGT GATACCAACC CCCTGAACGG GCAGTATATT
    TGCACCTGCC CTCAAGGCTT CAAAGGGGCT GATTGTACAG AAGATGTGGA TGAGTGTGCT
    ATGGCCAATA GCAATCCTTG TGAGCATGCA GGAAAGTGTG TGAACACAGA TGGTGCCTTC
    CACTGTGAAT GTCTGAAGGG CTATGCAGGG CCTCGTTGTG AGATGGACAT CAATGAGTGT
    CATTCCGACC CCTGCCAGAA TGATGCAACC TGTCTGGATA AGATTGGAGG CTTCACATGT
    TTGTGCATGC CAGGTTTCAA AGGTGTACAT TGTGAATTGG AGATAAACGA ATGTCAGAGC
    AACCCTTGTG TGAACAATGG TCAGTGTGTG GATAAAGTCA ATCGCTTCCA GTGTCTATGT
    CCACCTGGTT TCACTGGGCC AGTTTGCCAG ATTGATATTG ATGACTGTTC CAGTACTCCA
    TGTCTGAATG GGGCAAAGTG TATTGATCAC CCGAATGGCT ATGAATGCCA GTGTGCCACA
    GGATTCACTG GTGTGTTGTG TGAGGAGAAC ATTGACAACT GTGACCCTGA TCCTTGCCAC
    CATGGCCAGT GTCAGGATGG GATTGATTCC TACACCTGCA TCTGCAACCC GGGGTACATG
    GGTGCCATCT GCAGTGACCA GATAGATGAG TGTTACAGCA GCCCCTGCCT GAATGATGGT
    CGCTGCATTG ACCTGGTGAA TGGCTACCAG TGCAACTGCC AGCCTGGCAC ATCAGGTGTT
    AATTGTGAAA TCAATTTTGA TGACTGTGCA AGTAACCCTT GTGTCCATGG GGTCTGTATG
    GATGGCATTA ATCGTTACAG TTGTGTCTGC TCACCAGGAT TCACAGGACA GAGATGTAAC
    ATTGACATTG ATGAGTGTGC CTCCAATCCC TGTCGCAAGG GTGCAACATG CATCAATGAT
    GTGAATGGTT TCCGCTGTCT ATGCCCTGAG GGTCCTCATC GCCCCAGCTG CTACTCACAG
    GTGAACGAGT GCCTGAGCAG TCCCTGCATC CATGGAAACT GTACCGGGGG CCCTAGTGGA
    TACAAGTGCC TTTGTGATGC AGGCTGGATT GGTATCAACT GCGAAGTGGA CAAGAATGAA
    TGTCTTTCTA ACCCATGCCA GAATGGAGGA ACTTGTGACA ATCTGGTGAA TGGATACAGG
    TGTACTTGCA AGAAGGGCTT TAAAGGCTAT AACTGTCAGG TGAATATTGA TGAATGTGCT
    TCAAATCCGT GTCTGAACCA AGGAACCTGC TTTGATAACA TAAGTGGCTA CACTTGCCAC
    TGTGTGCTGC CATACACAGG CAAGAATTGT CAGACGGTAT TGGCTCCCTG TTCCCCAAAC
    CCATGTGAGA ATGCTGCTGT TTGCAAAGAG GCACCCGATT TTGAGAGTTA CACCTGCCTG
    TGTGCCCCTG GCTGGCAAGG TCAGCGGTGT ACAATTGACA TTGATGAATG TGTCTCCAAA
    CCCTGCATGA ACCATGGTCT CTGCCATAAT ACCCAGGGCA GCTACATGTG CGAGTGTCCT
    CCGGGCTTCA GTGGTATGGA CTGTGAGGAC GACATCGATG ACTGTCTTGC CAATCCTTGC
    CAGAATGGAG GTTCCTGTGT AGATGGAGTG AATACTTTCT CCTGCCTGTG TCATCCTGGC
    TTTATTGGAG ATAAGTGTCA AACAGACATG AATGAGTGTC TGAGTGAACC CTGTAAGAAT
    GGAGGGACTT GCTCTGACTA TGTCAACAGT TACACTTGCA AGTGCCAGGC GGGATTTGAT
    GGAGTACACT GTGAGAACAA CATCGACGAG TGCACTGAGA GCTCCTGTTT CAATGGTGGC
    ACATGTGTCG ACGGGATTAA CTCCTTCTCT TGCTTGTGCC CTTTGGGCTT TACTGGCCCC
    TTCTGCCTCC ATGAGATCAA TGAATGCAAC TCTCACCCAT GCCTAAATGA AGGAATATGT
    GTTGATGGCC TTGGTACCTA CCGCTGCACC TGTCCCTTAG GCTACACTGG GAAAAACTGT
    CAGACACTGG TGAACCTCTG TAGTCGGTCT CCATGTAAAA ACAAAGGTAC TTGCATTCAG
    GAAAAAGCAG AGTCCCGGTG CCTGTGTCCA TCAGGATGGG CTGGTGCTTA CTGTGACGTG
    CCCAATGTGT CCTGTGAGGT GGCGGCCTTA CACAGAGGGG TGTCCACTGA CCACCTGTGC
    CAGCACTCCG GCATCTGCAT CAATGCTGGC AACTCGCATC ACTGCCAGTG CCCCCTGGGC
    TACACCGGGA GCTACTGTGA GGAGCAGCTC GATGAGTGCT CATCCAACCC CTGCCAGCAT
    GGGGCCACCT GCAGGGACTT CATTGGCGGG TACAGATGTG AGTGTGTCCC AGGATATCAG
    GGCGTCAATT GCGAGTATGA AGTAGATGAG TGTCAGAATC AGCCATGCCA GAACGGAGGC
    ACCTGTGTCG ACCTCGTGAA CCATTTCAAG TGCTCCTGTC CACCAGGCAC TCGAGGCCTG
    CTTTGTGAAG AGAACATTGA TGACTGTGTT GGGGGTCCCC ACTGCCTCAA TGGTGGTCAG
    TGTGTGGATA GGATTGGGGG CTACAGTTGT CGCTGCTTGC CTGGCTTTGC GGGAGAGCGG
    TGTGAGGGCG ACATCAACGA GTGCCTCTCG AACCCCTGCA GCTCTGAGGG CAGCCTGGAC
    TGCATCCAGC TAACCAATGA CTACCTGTGT GTCTGTCGCA GTGCCTTCAC TGGTCGTCAC
    TGTGAAACTT TCATCGATGT GTGTCCCCAG ATGCCTTGTC TGAATGGGGG GACGTGTGCT
    GTGGCCAGCA ACATGCCTGA TGGTTTCATT TGTCGTTGTC CCCCGGGATT CTCCGGAGCG
    AAATGTCAGA GCAGCTGTGG ACAAGTGAAG TGCAGGAGAG GAGAGCAGTG TGTGCACACG
    GCCTCAGGAC CACGCTGCTT CTGCCCCAAC CCCCAGGACT GTGAATCAGG CTGTGCCAGT
    AGTCCCTGCC AACACGGGGG TAGCTGCTAT CCTCAGCGCC AGCCTCCTTA CTACTCTTGC
    CAGTGTTCTC CACCATTCTG GGGTAGCCAT TGTGAACTTT ACATGGCTCC CACCAGCACC
    CCTCCTGCCA CCTGCCTGAG CCAGTATTGT GCCGACAAAG CTCGGGACGG CGTCTGCGAT
    GAGGCCTGCA ACAGCCATGC CTGCCAGTGG GATGGGGGTG ACTGTTCCCT GACCATGGAG
    AACCCCTGGG CCAACTGCTC TTCCCCACTC CCCTGCTGGG ATTACATCAA CAACCAGTGT
    GATGAGCTGT GCAACACAGC GGAGTGCCTT TTTGACAATT TTGAATGCCA GGGGAACAGC
    AAGACATGCA AGTATGACAA ATACTGTGCA GACCACTTCA AAGACAACCA CTGTGACCAG
    GGATGCAACA GTGAGGAGTG TGGCTGGGAT GGGCTGGACT GTGCTGCTGA CCGGCCAGAG
    AACCTGGCAG AGGGGACCCT GGTTATAGTG GTGCTCATGC CCCCAGAGCA ACTGCTCCAG
    GATGCCCGCA GCTTCTTGCG GGCACTGGGC ACCCTGCTCC ATACCAACCT GCACATTAAA
    CGGGACTCCC AGGGGGCACT CATGGTCTAC CCCTATTATG GTGAGAAGTC AGCTGCCACA
    AAGAAGCAGA GGATGACTCG CAGATCCCTT CCCGGTGATC AAGAACAAGA GGTGGCTGGC
    TCTAAGGTAT TTCTGGAAAT TGACAACCGC CAGTGTGTTC AAGATTCAGA CCAATGTTTC
    AAGAACACAG ATGCAGCAGC AGCTCTTCTG GCTTCTCACG CCATCCAAGG GACCTTGTCC
    TACCCTCTTG TATCTGTTGT CAGTGAATCT CTGAATCCAA AACCCACTCA GCTTCTTTAT
    CTACTTGCTG TCGCTGTCGT CATTATCCTA TTTATTATCC TGCTGGGAGT TATCATGGCC
    AAAAGAAAAC GTAAGCATGG CTCCCTCTGG CTGCCTGAAG GTTTCACCCT TCGCCGAGAC
    TCCAGCAATC ACAAGCGTCG TGAACCAGTG GGACAAGATG CCGTGGGGCT GAAAAATCTC
    TCAGTGCAAG TCTCAGAGGC TAACCTAATT GGTTCTGGAA CAAGTGAACA TTGGGTTGAT
    GATGAAGGGC CCCAGCCAAA GAAAGCCAAG GCTGAAGATG AAGCTTTGCT CTCAGAAGAA
    GATGATGCCA TTGATCGACG TCCATGGACA CAGCAGCACC TTGAGGCTGC AGATATCCGC
    AGGACACCAT CACTGGCACT CACTCCTCCA CAGGCAGAGC AGGAGGTGGA CGTGCTGGAT
    GTGAATGTCC GTGGCCCAGA TGGGTGCACC CCATTGATGC TGGCTTCTCT CCGAGGAGGC
    AGCTCAGATA TGAGTGATGA AGATGAAGAC GGAGAAGACT CTTCTGCCAA CATCATCACA
    GACTTGGTCT ACCAGGGTGC CAGCCTGCAG GCCCAGACAG ACCGGACTGG TGAGATGGCC
    CTGCACCTTG CAGCCCGCTA CTCCAGGGCT GATGCTGCCA AGCGTCTCTT GGATGCAGGT
    GCAGATGCCA ATGCACAGGA CAACATGGGC CGTTGCCCTC TCCATGCTGC AGTGGCAGCT
    GATGCCCAGG GGGTCTTCCA GATTCTGATC CGCAACCGAG TAACTGATTT GGATGCCAGG
    ATGAACGACG GCACTACACC GCTGATCCTG GCTGCCCGCC TAGCTGTGGA GGGAATGGTG
    GCAGAACTAA TCAACTGCCA AGCAGATGTG AATGCAGTGG ATGACCATGG GAAATCTGCT
    CTTCACTGGG CAGCTGCTGT CAATAACGTG GAGGCAACCC TTTTGCTGTT GAAAAATGGG
    GCCAACCGAG ACATGCAAGA CAACAAGACA GATTACAAAG AAATACTTTT GAGGAAAGAA
    ACTTCGTCAA ACAGAATGCA TCACAAGACA GAGTCACTTT TCTGTTTACT AGTTGGTGAC
    AAATCGACAC TTGCTCAGGA CAGAGTGCCT TCCAGTGGAG ATGGAGTGTG TTTGCTAAAG
    CCAGGATTGA AAAGTGAGTT GCGAATCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.