ROCK2 cDNA ORF clone, Canis lupus familiaris(dog)

The following ROCK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROCK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe294217 XM_022404446.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe294218 XM_022404447.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe294219 XM_022404448.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe294220 XM_022404449.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe294221 XM_022404450.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe294222 XM_022404451.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe294223 XM_022404452.1
Latest version!
Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe40665 XM_014120231.1
Latest version!
Canis lupus familiaris Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe294217
    Clone ID Related Accession (Same CDS sequence) XM_022404446.1
    Accession Version XM_022404446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGATTGCTTG ATTTGGCATT TAAAGACTGG GATTGGTCAT TTGATGATGT TGATGGTGGT
    GATGATGATG ACGATGTTTT TGATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260154.1
    CDS163..4332
    Translation

    Target ORF information:

    RefSeq Version XM_022404446.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGATTGCTTG ATTTGGCATT TAAAGACTGG GATTGGTCAT TTGATGATGT TGATGGTGGT
    GATGATGATG ACGATGTTTT TGATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294218
    Clone ID Related Accession (Same CDS sequence) XM_022404447.1
    Accession Version XM_022404447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GAATCAAGAC TAGAAGGATG GCTTTCATTG CCTTTACGAA ACAACACTAA GAAATTTGGA
    TGGACTAAAA AGTATGTGAT TGTAAGCAGT AAGAAGATTC TTTTCTATGA CAGCGAACAA
    GATAAAGAAC AATCTAATCC TTACATGGTT TTAGATATAG ACAAATTATT TCATGTCCGA
    CCAGTTACAC AGACAGATGT ATATAGAGCA GATGCTAAAG ATATTCCAAG GATATTTCAG
    ATTCTGTACG CCAATGAAGG AGAAAGTAAG AAGGAACAAG AATTTCCAGT GGAGCCAGTA
    GGAGAAAAAT CAAATTATAT TTGCCATAAA GGACATGAAT TTATTCCTAC TCTGTATCAT
    TTCCCAACCA ACTGTGAGGC TTGTATGAAG CCATTGTGGC ATATGTTTAA ACCTCCTCCT
    GCTTTAGAAT GCCGGCGCTG CCATATTAAA TGTCATAAAG ATCACATGGA CAAAAAGGAG
    GAGATTATAG CACCTTGCAA GGTTTATTGT GATATTTCGA CGGCAAAGAA TCTATTGTTG
    TTAGCAAATT CTACAGAAGA GCAGCAGAAG TGGGTTAGTC GGTTGGTGAA AAAGATTCCT
    AAAAAGCCTC CAGCTCCAGA CCCTTTTGCA CGGTCATCAC CTAGAACTTC AATGAAGATA
    CAACAAAACC AGTCTATTAG ACGGCCAAGT CGACAGCTTG CCCCAAACAA ACCAAGATTG
    CTTGATTTGG CATTTAAAGA CTGGGATTGG TCATTTGATG ATGTTGATGG TGGTGATGAT
    GATGACGATG TTTTTGATTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260155.1
    CDS163..4326
    Translation

    Target ORF information:

    RefSeq Version XM_022404447.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GAATCAAGAC TAGAAGGATG GCTTTCATTG CCTTTACGAA ACAACACTAA GAAATTTGGA
    TGGACTAAAA AGTATGTGAT TGTAAGCAGT AAGAAGATTC TTTTCTATGA CAGCGAACAA
    GATAAAGAAC AATCTAATCC TTACATGGTT TTAGATATAG ACAAATTATT TCATGTCCGA
    CCAGTTACAC AGACAGATGT ATATAGAGCA GATGCTAAAG ATATTCCAAG GATATTTCAG
    ATTCTGTACG CCAATGAAGG AGAAAGTAAG AAGGAACAAG AATTTCCAGT GGAGCCAGTA
    GGAGAAAAAT CAAATTATAT TTGCCATAAA GGACATGAAT TTATTCCTAC TCTGTATCAT
    TTCCCAACCA ACTGTGAGGC TTGTATGAAG CCATTGTGGC ATATGTTTAA ACCTCCTCCT
    GCTTTAGAAT GCCGGCGCTG CCATATTAAA TGTCATAAAG ATCACATGGA CAAAAAGGAG
    GAGATTATAG CACCTTGCAA GGTTTATTGT GATATTTCGA CGGCAAAGAA TCTATTGTTG
    TTAGCAAATT CTACAGAAGA GCAGCAGAAG TGGGTTAGTC GGTTGGTGAA AAAGATTCCT
    AAAAAGCCTC CAGCTCCAGA CCCTTTTGCA CGGTCATCAC CTAGAACTTC AATGAAGATA
    CAACAAAACC AGTCTATTAG ACGGCCAAGT CGACAGCTTG CCCCAAACAA ACCAAGATTG
    CTTGATTTGG CATTTAAAGA CTGGGATTGG TCATTTGATG ATGTTGATGG TGGTGATGAT
    GATGACGATG TTTTTGATTT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294219
    Clone ID Related Accession (Same CDS sequence) XM_022404448.1
    Accession Version XM_022404448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4158bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAATTCAGAA AAAATTATAT
    ACATTAGAAG AACACCTCAG CACTGAGATA CAAGCCAAAG AAGAACTAGA GCAAAAGTGC
    AAATCTGTTA ATACTCGACT TGAAAAAGTA GCAAAGGAAT TAGAAGAAGA GATTACTTTA
    AGGAAAAATG TGGAATCAGC ATTAAGACAA TTAGAAAGAG AAAAGGCACT TCTTCAGCAC
    AAAAATGCAG AATATCAGCG GAAAGCTGAT CACGAAGCAG ACAAGAAGCG GAATTTGGAA
    AATGATGTTA ACAGTTTAAA GGATCAACTT GAAGATTTGA AAAAAAGAAA TCAGAACTCT
    CAAATATCCA CCGAGAAAGT GAATCAGCTC CAGAGACAAC TGGATGAAAC CAATGCTTTA
    CTGAGAACAG AATCTGATAC TACGGCCCGG TTAAGAAAAA CCCAGGCAGA AAGTTCAAAA
    CAGATTCAGC AGCTGGAATC TAACAATAGA GATCTCCAAG ATAAAAATTG CCTGCTAGAG
    ACTGCCAAGT TAAAACTTGA AAAGGAATTT ATGAATCTTC AGTCAGTTCT AGAATCTGAA
    AGGAGGGACC GAACCCACGG ATCCGAGATA ATTAATGACT TACAAGGTAG AATATCTGGC
    CTAGAAGAAG ACTTAAAGAA TGGAAAAATC TTATTAGCAA AAGCAGAGGT GGAGAAGAGA
    CAACTGCAGG AGAGACTTAC TGATTTGGAA AAGGAAAAGA ACAACATGGA AATAGATATG
    ACGTACCAAC TAAAAGTTAT ACAGCAGAGC TTAGAACAAG AAGAAGCTGA GCATAAGGCC
    ACAAAAGCAC GGCTGGCAGA TAAAAATAAG ATTTATGAGT CCATTGAAGA AGCAAAATCA
    GAAGCCATGA AAGAAATGGA GAAGAAGCTC TCAGAGGAAA GGACGTTAAA ACAGAAGGTG
    GAGAATCTAT TGCTGGAAGC TGAGAAAAGA TGCTCTTTAT TTGACTGTGA CCTCAAGCAG
    TCACAGCAGA AAATAAATGA ACTCCTTAAA CAGAAAGATG TGCTAAATGA GGATGTTAGA
    AACTTGACAT TAAAAATAGA GCAGGAAACT CAGAAGCGCT GCCTTACACA AAATGACTTG
    AAGATGCAAA CACAGCAAGT TAACACACTA AAAATGTCAG AAAAACAGTT AAAACAAGAG
    AATAATCATC TCATGGAAAT GAAAATGAGC TTGGAAAAGC AGAATGCTGA ACTTCGGAAA
    GAACGTCAAG ATGCAGATGG GCAAATGAAA GAACTTCAGG ATCAGCTTGA AGCGGAGCAG
    TATTTCTCAA CCCTCTATAA AACACAGGTG AGGGAACTTA AAGAAGAATG TGAAGAAAAA
    ACCAAACTTT GTAAAGAATT ACAGCAAAAG AAACAGGAAT TACAGGAAGA AAGGGATTCC
    TTGGCTGCTC AACTGGAGAT CACCTTGACC AAAGCAGATT CTGAGCAGCT GGCTCGTTCA
    ATTGCTGAAG AGCAGTATTC TGATTTGGAA AAAGAGAAAA TTATGAAGGA GCTTGAGATC
    AAAGAGATGA TGGCTAGACA CAAACAGGAA CTCACTGAAA AAGATGCTAC AATTGCATCT
    CTTGAAGAAA CGAATAGAAC ACTAACTAGT GATGTTGCCA ATCTTGCAAA TGAGAAAGAA
    GAATTAAATA ACAAATTGAA AGATGCACAA GAACAGCTGT CACGGCTGAA GGATGAGGAG
    ATCAGTGCAG CTGCTATTAA AGCACAGTTT GAGAAGCAGC TGCTCACAGA GAGAACACTG
    AAAACTCAAG CTGTGAATAA GTTGGCTGAG ATCATGAATC GAAAAGAACC TGTCAAGCGT
    GGTAGTGACA CTGATGTGCG GAGAAAAGAG AAAGAAAATC GGAAGCTACA TATGGAGCTT
    AAATCTGAAC GTGAAAAACT GACCCAGCAG ATGATCAAGT ACCAGAAAGA ACTGAATGAA
    ATGCAGGCTC AAATAGCTGA AGAGAGCCAG ATTCGAATTG AACTGCAGAT GACACTGGAC
    AGTAAAGATA GTGACATTGA GCAGCTGCGG TCACAGCTCC AGGCTTTGCA TATTGGTCTG
    GATAGTTCCA GTATAGGCAG TGGACCGGGG GACGCTGAGG CAGATGATGG GTTTCCAGTT
    CATATCACTC AATCACGCAC TATGGAATCC ATGTCATTCA CCTACCAGCG TTCCTCTACT
    TCTCTCAATA TTGCCACTAA GCCTTCCAGT TCTCATATGC TTCTTGACTC TGATTCTGAC
    TCAGAAGAAG AATCTTTGCC TTACTTACCT ATTTCATCGG AACCTAATGG TACAGAATCA
    AGACTAGAAG GATGGCTTTC ATTGCCTTTA CGAAACAACA CTAAGAAATT TGGATGGACT
    AAAAAGTATG TGATTGTAAG CAGTAAGAAG ATTCTTTTCT ATGACAGCGA ACAAGATAAA
    GAACAATCTA ATCCTTACAT GGTTTTAGAT ATAGACAAAT TATTTCATGT CCGACCAGTT
    ACACAGACAG ATGTATATAG AGCAGATGCT AAAGATATTC CAAGGATATT TCAGATTCTG
    TACGCCAATG AAGGAGAAAG TAAGAAGGAA CAAGAATTTC CAGTGGAGCC AGTAGGAGAA
    AAATCAAATT ATATTTGCCA TAAAGGACAT GAATTTATTC CTACTCTGTA TCATTTCCCA
    ACCAACTGTG AGGCTTGTAT GAAGCCATTG TGGCATATGT TTAAACCTCC TCCTGCTTTA
    GAATGCCGGC GCTGCCATAT TAAATGTCAT AAAGATCACA TGGACAAAAA GGAGGAGATT
    ATAGCACCTT GCAAGGTTTA TTGTGATATT TCGACGGCAA AGAATCTATT GTTGTTAGCA
    AATTCTACAG AAGAGCAGCA GAAGTGGGTT AGTCGGTTGG TGAAAAAGAT TCCTAAAAAG
    CCTCCAGCTC CAGACCCTTT TGCACGGTCA TCACCTAGAA CTTCAATGAA GATACAACAA
    AACCAGTCTA TTAGACGGCC AAGTCGACAG CTTGCCCCAA ACAAACCAAG ATTGCTTGAT
    TTGGCATTTA AAGACTGGGA TTGGTCATTT GATGATGTTG ATGGTGGTGA TGATGATGAC
    GATGTTTTTG ATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260156.1
    CDS163..4320
    Translation

    Target ORF information:

    RefSeq Version XM_022404448.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAATTCAGAA AAAATTATAT
    ACATTAGAAG AACACCTCAG CACTGAGATA CAAGCCAAAG AAGAACTAGA GCAAAAGTGC
    AAATCTGTTA ATACTCGACT TGAAAAAGTA GCAAAGGAAT TAGAAGAAGA GATTACTTTA
    AGGAAAAATG TGGAATCAGC ATTAAGACAA TTAGAAAGAG AAAAGGCACT TCTTCAGCAC
    AAAAATGCAG AATATCAGCG GAAAGCTGAT CACGAAGCAG ACAAGAAGCG GAATTTGGAA
    AATGATGTTA ACAGTTTAAA GGATCAACTT GAAGATTTGA AAAAAAGAAA TCAGAACTCT
    CAAATATCCA CCGAGAAAGT GAATCAGCTC CAGAGACAAC TGGATGAAAC CAATGCTTTA
    CTGAGAACAG AATCTGATAC TACGGCCCGG TTAAGAAAAA CCCAGGCAGA AAGTTCAAAA
    CAGATTCAGC AGCTGGAATC TAACAATAGA GATCTCCAAG ATAAAAATTG CCTGCTAGAG
    ACTGCCAAGT TAAAACTTGA AAAGGAATTT ATGAATCTTC AGTCAGTTCT AGAATCTGAA
    AGGAGGGACC GAACCCACGG ATCCGAGATA ATTAATGACT TACAAGGTAG AATATCTGGC
    CTAGAAGAAG ACTTAAAGAA TGGAAAAATC TTATTAGCAA AAGCAGAGGT GGAGAAGAGA
    CAACTGCAGG AGAGACTTAC TGATTTGGAA AAGGAAAAGA ACAACATGGA AATAGATATG
    ACGTACCAAC TAAAAGTTAT ACAGCAGAGC TTAGAACAAG AAGAAGCTGA GCATAAGGCC
    ACAAAAGCAC GGCTGGCAGA TAAAAATAAG ATTTATGAGT CCATTGAAGA AGCAAAATCA
    GAAGCCATGA AAGAAATGGA GAAGAAGCTC TCAGAGGAAA GGACGTTAAA ACAGAAGGTG
    GAGAATCTAT TGCTGGAAGC TGAGAAAAGA TGCTCTTTAT TTGACTGTGA CCTCAAGCAG
    TCACAGCAGA AAATAAATGA ACTCCTTAAA CAGAAAGATG TGCTAAATGA GGATGTTAGA
    AACTTGACAT TAAAAATAGA GCAGGAAACT CAGAAGCGCT GCCTTACACA AAATGACTTG
    AAGATGCAAA CACAGCAAGT TAACACACTA AAAATGTCAG AAAAACAGTT AAAACAAGAG
    AATAATCATC TCATGGAAAT GAAAATGAGC TTGGAAAAGC AGAATGCTGA ACTTCGGAAA
    GAACGTCAAG ATGCAGATGG GCAAATGAAA GAACTTCAGG ATCAGCTTGA AGCGGAGCAG
    TATTTCTCAA CCCTCTATAA AACACAGGTG AGGGAACTTA AAGAAGAATG TGAAGAAAAA
    ACCAAACTTT GTAAAGAATT ACAGCAAAAG AAACAGGAAT TACAGGAAGA AAGGGATTCC
    TTGGCTGCTC AACTGGAGAT CACCTTGACC AAAGCAGATT CTGAGCAGCT GGCTCGTTCA
    ATTGCTGAAG AGCAGTATTC TGATTTGGAA AAAGAGAAAA TTATGAAGGA GCTTGAGATC
    AAAGAGATGA TGGCTAGACA CAAACAGGAA CTCACTGAAA AAGATGCTAC AATTGCATCT
    CTTGAAGAAA CGAATAGAAC ACTAACTAGT GATGTTGCCA ATCTTGCAAA TGAGAAAGAA
    GAATTAAATA ACAAATTGAA AGATGCACAA GAACAGCTGT CACGGCTGAA GGATGAGGAG
    ATCAGTGCAG CTGCTATTAA AGCACAGTTT GAGAAGCAGC TGCTCACAGA GAGAACACTG
    AAAACTCAAG CTGTGAATAA GTTGGCTGAG ATCATGAATC GAAAAGAACC TGTCAAGCGT
    GGTAGTGACA CTGATGTGCG GAGAAAAGAG AAAGAAAATC GGAAGCTACA TATGGAGCTT
    AAATCTGAAC GTGAAAAACT GACCCAGCAG ATGATCAAGT ACCAGAAAGA ACTGAATGAA
    ATGCAGGCTC AAATAGCTGA AGAGAGCCAG ATTCGAATTG AACTGCAGAT GACACTGGAC
    AGTAAAGATA GTGACATTGA GCAGCTGCGG TCACAGCTCC AGGCTTTGCA TATTGGTCTG
    GATAGTTCCA GTATAGGCAG TGGACCGGGG GACGCTGAGG CAGATGATGG GTTTCCAGTT
    CATATCACTC AATCACGCAC TATGGAATCC ATGTCATTCA CCTACCAGCG TTCCTCTACT
    TCTCTCAATA TTGCCACTAA GCCTTCCAGT TCTCATATGC TTCTTGACTC TGATTCTGAC
    TCAGAAGAAG AATCTTTGCC TTACTTACCT ATTTCATCGG AACCTAATGG TACAGAATCA
    AGACTAGAAG GATGGCTTTC ATTGCCTTTA CGAAACAACA CTAAGAAATT TGGATGGACT
    AAAAAGTATG TGATTGTAAG CAGTAAGAAG ATTCTTTTCT ATGACAGCGA ACAAGATAAA
    GAACAATCTA ATCCTTACAT GGTTTTAGAT ATAGACAAAT TATTTCATGT CCGACCAGTT
    ACACAGACAG ATGTATATAG AGCAGATGCT AAAGATATTC CAAGGATATT TCAGATTCTG
    TACGCCAATG AAGGAGAAAG TAAGAAGGAA CAAGAATTTC CAGTGGAGCC AGTAGGAGAA
    AAATCAAATT ATATTTGCCA TAAAGGACAT GAATTTATTC CTACTCTGTA TCATTTCCCA
    ACCAACTGTG AGGCTTGTAT GAAGCCATTG TGGCATATGT TTAAACCTCC TCCTGCTTTA
    GAATGCCGGC GCTGCCATAT TAAATGTCAT AAAGATCACA TGGACAAAAA GGAGGAGATT
    ATAGCACCTT GCAAGGTTTA TTGTGATATT TCGACGGCAA AGAATCTATT GTTGTTAGCA
    AATTCTACAG AAGAGCAGCA GAAGTGGGTT AGTCGGTTGG TGAAAAAGAT TCCTAAAAAG
    CCTCCAGCTC CAGACCCTTT TGCACGGTCA TCACCTAGAA CTTCAATGAA GATACAACAA
    AACCAGTCTA TTAGACGGCC AAGTCGACAG CTTGCCCCAA ACAAACCAAG ATTGCTTGAT
    TTGGCATTTA AAGACTGGGA TTGGTCATTT GATGATGTTG ATGGTGGTGA TGATGATGAC
    GATGTTTTTG ATTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294220
    Clone ID Related Accession (Same CDS sequence) XM_022404449.1
    Accession Version XM_022404449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGAAGCACGA GGGGAACAAA GGAAAGCATG GAGAATTCTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260157.1
    CDS162..4286
    Translation

    Target ORF information:

    RefSeq Version XM_022404449.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGAAGCACGA GGGGAACAAA GGAAAGCATG GAGAATTCTG CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294221
    Clone ID Related Accession (Same CDS sequence) XM_022404450.1
    Accession Version XM_022404450.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGTTCTGGCC CTGCCCTTCG AATCATTCTT CATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260158.1
    CDS162..4277
    Translation

    Target ORF information:

    RefSeq Version XM_022404450.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGTTCTGGCC CTGCCCTTCG AATCATTCTT CATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294222
    Clone ID Related Accession (Same CDS sequence) XM_022404451.1
    Accession Version XM_022404451.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260159.1
    CDS162..4247
    Translation

    Target ORF information:

    RefSeq Version XM_022404451.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404451.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG TTCATATCAC TCAATCACGC ACTATGGAAT CCATGTCATT CACCTACCAG
    CGTTCCTCTA CTTCTCTCAA TATTGCCACT AAGCCTTCCA GTTCTCATAT GCTTCTTGAC
    TCTGATTCTG ACTCAGAAGA AGAATCTTTG CCTTACTTAC CTATTTCATC GGAACCTAAT
    GGTACAGAAT CAAGACTAGA AGGATGGCTT TCATTGCCTT TACGAAACAA CACTAAGAAA
    TTTGGATGGA CTAAAAAGTA TGTGATTGTA AGCAGTAAGA AGATTCTTTT CTATGACAGC
    GAACAAGATA AAGAACAATC TAATCCTTAC ATGGTTTTAG ATATAGACAA ATTATTTCAT
    GTCCGACCAG TTACACAGAC AGATGTATAT AGAGCAGATG CTAAAGATAT TCCAAGGATA
    TTTCAGATTC TGTACGCCAA TGAAGGAGAA AGTAAGAAGG AACAAGAATT TCCAGTGGAG
    CCAGTAGGAG AAAAATCAAA TTATATTTGC CATAAAGGAC ATGAATTTAT TCCTACTCTG
    TATCATTTCC CAACCAACTG TGAGGCTTGT ATGAAGCCAT TGTGGCATAT GTTTAAACCT
    CCTCCTGCTT TAGAATGCCG GCGCTGCCAT ATTAAATGTC ATAAAGATCA CATGGACAAA
    AAGGAGGAGA TTATAGCACC TTGCAAGGTT TATTGTGATA TTTCGACGGC AAAGAATCTA
    TTGTTGTTAG CAAATTCTAC AGAAGAGCAG CAGAAGTGGG TTAGTCGGTT GGTGAAAAAG
    ATTCCTAAAA AGCCTCCAGC TCCAGACCCT TTTGCACGGT CATCACCTAG AACTTCAATG
    AAGATACAAC AAAACCAGTC TATTAGACGG CCAAGTCGAC AGCTTGCCCC AAACAAACCA
    AGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe294223
    Clone ID Related Accession (Same CDS sequence) XM_022404452.1
    Accession Version XM_022404452.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3993bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho-associated protein kinase 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG AATCAAGACT AGAAGGATGG CTTTCATTGC CTTTACGAAA CAACACTAAG
    AAATTTGGAT GGACTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC
    AGCGAACAAG ATAAAGAACA ATCTAATCCT TACATGGTTT TAGATATAGA CAAATTATTT
    CATGTCCGAC CAGTTACACA GACAGATGTA TATAGAGCAG ATGCTAAAGA TATTCCAAGG
    ATATTTCAGA TTCTGTACGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG
    GAGCCAGTAG GAGAAAAATC AAATTATATT TGCCATAAAG GACATGAATT TATTCCTACT
    CTGTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CATTGTGGCA TATGTTTAAA
    CCTCCTCCTG CTTTAGAATG CCGGCGCTGC CATATTAAAT GTCATAAAGA TCACATGGAC
    AAAAAGGAGG AGATTATAGC ACCTTGCAAG GTTTATTGTG ATATTTCGAC GGCAAAGAAT
    CTATTGTTGT TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA
    AAGATTCCTA AAAAGCCTCC AGCTCCAGAC CCTTTTGCAC GGTCATCACC TAGAACTTCA
    ATGAAGATAC AACAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA
    CCAAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT
    GGTGATGATG ATGACGATGT TTTTGATTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022260160.1
    CDS162..4154
    Translation

    Target ORF information:

    RefSeq Version XM_022404452.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022404452.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAAGGCAG AAGACTATGA TGTTGTAAAA GTTATTGGAA GAGGTGCTTT TGGTGAGGTG 
    CAGCTGGTTC GTCATAAAGC ATCACAGAAG GTGTATGCTA TGAAGCTTCT TAGTAAGTTT
    GAAATGATAA AAAGATCAGA TTCTGCTTTT TTCTGGGAAG AGAGAGATAT TATGGCCTTT
    GCCAACAGTC CCTGGGTGGT TCAGCTCTTT TGTGCTTTTC AAGATGATAA GTATCTGTAC
    ATGGTAATGG AGTACATGCC TGGTGGAGAT CTTGTAAACC TTATGAGTAA CTATGACGTG
    CCTGAAAAAT GGGCCAAATT CTATACTGCT GAAGTTGTTC TTGCTCTGGA TGCCATACAC
    TCCATGGGCT TAATTCACAG AGATGTAAAG CCTGACAACA TGCTCTTGGA TAAACATGGA
    CATCTAAAGT TAGCAGATTT TGGCACATGT ATGAAGATGG ATGAAACAGG CATGGTGCAT
    TGTGATACAG CAGTTGGAAC ACCTGATTAT ATTTCACCTG AGGTTCTCAA ATCACAAGGG
    GGTGATGGTT ACTATGGGCG AGAATGTGAT TGGTGGTCGG TGGGTGTTTT CCTTTTTGAG
    ATGCTGGTGG GGGATACTCC ATTTTATGCA GATTCACTTG TAGGAACTTA TAGCAAAATT
    ATGGATCATA AAAACTCACT GTGTTTCCCT GAAGATGCAG AAATTTCTAA ACATGCGAAG
    AATCTCATTT GCGCCTTCTT AACAGATAGG GAGGTACGAC TTGGGAGAAA TGGGGTAGAA
    GAAATCAAAC AGCATCCTTT CTTTAAGAAT GATCAATGGA ATTGGGATAA CATAAGAGAG
    ACGGCAGCTC CTGTAGTACC AGAACTCAGC AGTGACATAG ACAGCAGCAA TTTTGATGAC
    ATCGAAGATG ATAAAGGAGA TGTAGAAACT TTTCCAATTC CTAAAGCTTT TGTGGGAAAT
    CAGCTGCCTT TTATAGGATT TACCTACTAT AGAGAAAATC TGTTATTAAG TGAGTCTTCA
    TCTTGTAGAG AAAATGATTC ATTACAATCA AGGAAAAATG AAGAAAGTCA AGAGATTCAG
    AAAAAATTAT ATACATTAGA AGAACACCTC AGCACTGAGA TACAAGCCAA AGAAGAACTA
    GAGCAAAAGT GCAAATCTGT TAATACTCGA CTTGAAAAAG TAGCAAAGGA ATTAGAAGAA
    GAGATTACTT TAAGGAAAAA TGTGGAATCA GCATTAAGAC AATTAGAAAG AGAAAAGGCA
    CTTCTTCAGC ACAAAAATGC AGAATATCAG CGGAAAGCTG ATCACGAAGC AGACAAGAAG
    CGGAATTTGG AAAATGATGT TAACAGTTTA AAGGATCAAC TTGAAGATTT GAAAAAAAGA
    AATCAGAACT CTCAAATATC CACCGAGAAA GTGAATCAGC TCCAGAGACA ACTGGATGAA
    ACCAATGCTT TACTGAGAAC AGAATCTGAT ACTACGGCCC GGTTAAGAAA AACCCAGGCA
    GAAAGTTCAA AACAGATTCA GCAGCTGGAA TCTAACAATA GAGATCTCCA AGATAAAAAT
    TGCCTGCTAG AGACTGCCAA GTTAAAACTT GAAAAGGAAT TTATGAATCT TCAGTCAGTT
    CTAGAATCTG AAAGGAGGGA CCGAACCCAC GGATCCGAGA TAATTAATGA CTTACAAGGT
    AGAATATCTG GCCTAGAAGA AGACTTAAAG AATGGAAAAA TCTTATTAGC AAAAGCAGAG
    GTGGAGAAGA GACAACTGCA GGAGAGACTT ACTGATTTGG AAAAGGAAAA GAACAACATG
    GAAATAGATA TGACGTACCA ACTAAAAGTT ATACAGCAGA GCTTAGAACA AGAAGAAGCT
    GAGCATAAGG CCACAAAAGC ACGGCTGGCA GATAAAAATA AGATTTATGA GTCCATTGAA
    GAAGCAAAAT CAGAAGCCAT GAAAGAAATG GAGAAGAAGC TCTCAGAGGA AAGGACGTTA
    AAACAGAAGG TGGAGAATCT ATTGCTGGAA GCTGAGAAAA GATGCTCTTT ATTTGACTGT
    GACCTCAAGC AGTCACAGCA GAAAATAAAT GAACTCCTTA AACAGAAAGA TGTGCTAAAT
    GAGGATGTTA GAAACTTGAC ATTAAAAATA GAGCAGGAAA CTCAGAAGCG CTGCCTTACA
    CAAAATGACT TGAAGATGCA AACACAGCAA GTTAACACAC TAAAAATGTC AGAAAAACAG
    TTAAAACAAG AGAATAATCA TCTCATGGAA ATGAAAATGA GCTTGGAAAA GCAGAATGCT
    GAACTTCGGA AAGAACGTCA AGATGCAGAT GGGCAAATGA AAGAACTTCA GGATCAGCTT
    GAAGCGGAGC AGTATTTCTC AACCCTCTAT AAAACACAGG TGAGGGAACT TAAAGAAGAA
    TGTGAAGAAA AAACCAAACT TTGTAAAGAA TTACAGCAAA AGAAACAGGA ATTACAGGAA
    GAAAGGGATT CCTTGGCTGC TCAACTGGAG ATCACCTTGA CCAAAGCAGA TTCTGAGCAG
    CTGGCTCGTT CAATTGCTGA AGAGCAGTAT TCTGATTTGG AAAAAGAGAA AATTATGAAG
    GAGCTTGAGA TCAAAGAGAT GATGGCTAGA CACAAACAGG AACTCACTGA AAAAGATGCT
    ACAATTGCAT CTCTTGAAGA AACGAATAGA ACACTAACTA GTGATGTTGC CAATCTTGCA
    AATGAGAAAG AAGAATTAAA TAACAAATTG AAAGATGCAC AAGAACAGCT GTCACGGCTG
    AAGGATGAGG AGATCAGTGC AGCTGCTATT AAAGCACAGT TTGAGAAGCA GCTGCTCACA
    GAGAGAACAC TGAAAACTCA AGCTGTGAAT AAGTTGGCTG AGATCATGAA TCGAAAAGAA
    CCTGTCAAGC GTGGTAGTGA CACTGATGTG CGGAGAAAAG AGAAAGAAAA TCGGAAGCTA
    CATATGGAGC TTAAATCTGA ACGTGAAAAA CTGACCCAGC AGATGATCAA GTACCAGAAA
    GAACTGAATG AAATGCAGGC TCAAATAGCT GAAGAGAGCC AGATTCGAAT TGAACTGCAG
    ATGACACTGG ACAGTAAAGA TAGTGACATT GAGCAGCTGC GGTCACAGCT CCAGGCTTTG
    CATATTGGTC TGGATAGTTC CAGTATAGGC AGTGGACCGG GGGACGCTGA GGCAGATGAT
    GGGTTTCCAG AATCAAGACT AGAAGGATGG CTTTCATTGC CTTTACGAAA CAACACTAAG
    AAATTTGGAT GGACTAAAAA GTATGTGATT GTAAGCAGTA AGAAGATTCT TTTCTATGAC
    AGCGAACAAG ATAAAGAACA ATCTAATCCT TACATGGTTT TAGATATAGA CAAATTATTT
    CATGTCCGAC CAGTTACACA GACAGATGTA TATAGAGCAG ATGCTAAAGA TATTCCAAGG
    ATATTTCAGA TTCTGTACGC CAATGAAGGA GAAAGTAAGA AGGAACAAGA ATTTCCAGTG
    GAGCCAGTAG GAGAAAAATC AAATTATATT TGCCATAAAG GACATGAATT TATTCCTACT
    CTGTATCATT TCCCAACCAA CTGTGAGGCT TGTATGAAGC CATTGTGGCA TATGTTTAAA
    CCTCCTCCTG CTTTAGAATG CCGGCGCTGC CATATTAAAT GTCATAAAGA TCACATGGAC
    AAAAAGGAGG AGATTATAGC ACCTTGCAAG GTTTATTGTG ATATTTCGAC GGCAAAGAAT
    CTATTGTTGT TAGCAAATTC TACAGAAGAG CAGCAGAAGT GGGTTAGTCG GTTGGTGAAA
    AAGATTCCTA AAAAGCCTCC AGCTCCAGAC CCTTTTGCAC GGTCATCACC TAGAACTTCA
    ATGAAGATAC AACAAAACCA GTCTATTAGA CGGCCAAGTC GACAGCTTGC CCCAAACAAA
    CCAAGATTGC TTGATTTGGC ATTTAAAGAC TGGGATTGGT CATTTGATGA TGTTGATGGT
    GGTGATGATG ATGACGATGT TTTTGATTTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40665
    Clone ID Related Accession (Same CDS sequence) XM_014120231.1
    Accession Version XM_014120231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho-associated protein kinase 2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCATTCC ACACGGATGG AAACTTGCTT AACCAAAGAG CGAAGAAGAG AAGAGCCAAG 
    TATGTTCAGT TTGAGAAAAC CAGCATACCT GCCAGGACTC TTTCTGTTAA TGTGACAGAG
    CTTGCTCGGA TTATTCCCCA GTTGTTGGGA CAACTAGGCC CCTTCCCAGA TTGCGCCCTG
    CGAGGATCGG ACCAATGGCA CGAGCTGCGA GCCGGCAGGA ATGCGGAGGA GCTGCCCCGT
    AACCTGCCCT TCTTCCGCCC CCCAAAGGCA AGGAGGAGCT GGAGAGGGCC TGGGGACCGA
    GGCCTCTTCT ATCACCCTTT TATGCTCCTC CGCGGATGGG TGCTCAGGCC CGGGGCCGCG
    AGAAGACCGC TAGGGACCTG CACAAAGGCT CTCGCATCCC AGCACTTCTG CCCCGGGGGC
    GAAGCCGGGC GGGGAGTGCG GTGGCCGACC CCGCTGCGCG CCCCTCCGGC CCGTCCGCGG
    CCCTCCCCCC GCCCCGGGCC CAGGCAGGGG GCAGGGGCGC CCTGCAGCCT CCAGGCCAGC
    GGCATGCGGC GCCGGGCCGT GGGTCGGGGT CGGGGTCGGG GCCGGGGAAA CCCGCCGCGC
    GCCCTCTGGG GAGATGACAA AGAGCCGGGG CCAAGGAATC CGTCCGAGCT GTCGAGGAGA
    AGGGATCTCT CCTTCTCGCG GTCCGTCCTT CCTCAGGCAG CCCGTGGAAG ACCCCTGTCC
    CTAGTCAGCA CTCCGCGCGG GTTGGCCACG GGGCAGGACC TGGCTGAAGA CCGCCTCTCG
    GTGCAGGAGC GGGACGTGGT GATGGGCTGC GTCCCGTCCG ACGTCCTCGC CCTGGCGTCT
    CCCCGATTTT CGCGTCAGCG TGTGGATGGC TTAAATTCCT TGGTCCTTGA TTTGGATTTT
    CCTGCTTTGA GGAAAAACAA GAATATAGAT AATTTTTTAA ATAGATATGA GAAAATTGTG
    AAAAAAATCA GAGGTCTACA GATGAAGGCA GAAGACTATG ATGTTGTAAA AGTTATTGGA
    AGAGGTGCTT TTGGTGAGGT GCAGCTGGTT CGTCATAAAG CATCACAGAA GGTGTATGCT
    ATGAAGCTTC TTAGTAAGTT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA
    GAGAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTGG TTCAGCTCTT TTGTGCTTTT
    CAAGATGATA AGTATCTGTA CATGGTAATG GAGTACATGC CTGGTGGAGA TCTTGTAAAC
    CTTATGAGTA ACTATGACGT GCCTGAAAAA TGGGCCAAAT TCTATACTGC TGAAGTTGTT
    CTTGCTCTGG ATGCCATACA CTCCATGGGC TTAATTCACA GAGATGTAAA GCCTGACAAC
    ATGCTCTTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAGATG
    GATGAAACAG GCATGGTGCA TTGTGATACA GCAGTTGGAA CACCTGATTA TATTTCACCT
    GAGGTTCTCA AATCACAAGG GGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCG
    GTGGGTGTTT TCCTTTTTGA GATGCTGGTG GGGGATACTC CATTTTATGC AGATTCACTT
    GTAGGAACTT ATAGCAAAAT TATGGATCAT AAAAACTCAC TGTGTTTCCC TGAAGATGCA
    GAAATTTCTA AACATGCGAA GAATCTCATT TGCGCCTTCT TAACAGATAG GGAGGTACGA
    CTTGGGAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG
    AATTGGGATA ACATAAGAGA GACGGCAGCT CCTGTAGTAC CAGAACTCAG CAGTGACATA
    GACAGCAGCA ATTTTGATGA CATCGAAGAT GATAAAGGAG ATGTAGAAAC TTTTCCAATT
    CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTA TAGAGAAAAT
    CTGTTATTAA GTGAGTCTTC ATCTTGTAGA GAAAATGATT CATTACAATC AAGGAAAAAT
    GAAGAAAGTC AAGAGATTCA GAAAAAATTA TATACATTAG AAGAACACCT CAGCACTGAG
    ATACAAGCCA AAGAAGAACT AGAGCAAAAG TGCAAATCTG TTAATACTCG ACTTGAAAAA
    GTAGCAAAGG AATTAGAAGA AGAGATTACT TTAAGGAAAA ATGTGGAATC AGCATTAAGA
    CAATTAGAAA GAGAAAAGGC ACTTCTTCAG CACAAAAATG CAGAATATCA GCGGAAAGCT
    GATCACGAAG CAGACAAGAA GCGGAATTTG GAAAATGATG TTAACAGTTT AAAGGATCAA
    CTTGAAGATT TGAAAAAAAG AAATCAGAAC TCTCAAATAT CCACCGAGAA AGTGAATCAG
    CTCCAGAGAC AACTGGATGA AACCAATGCT TTACTGAGAA CAGAATCTGA TACTACGGCC
    CGGTTAAGAA AAACCCAGGC AGAAAGTTCA AAACAGATTC AGCAGCTGGA ATCTAACAAT
    AGAGATCTCC AAGATAAAAA TTGCCTGCTA GAGACTGCCA AGTTAAAACT TGAAAAGGAA
    TTTATGAATC TTCAGTCAGT TCTAGAATCT GAAAGGAGGG ACCGAACCCA CGGATCCGAG
    ATAATTAATG ACTTACAAGG TAGAATATCT GGCCTAGAAG AAGACTTAAA GAATGGAAAA
    ATCTTATTAG CAAAAGCAGA GGTGGAGAAG AGACAACTGC AGGAGAGACT TACTGATTTG
    GAAAAGGAAA AGAACAACAT GGAAATAGAT ATGACGTACC AACTAAAAGT TATACAGCAG
    AGCTTAGAAC AAGAAGAAGC TGAGCATAAG GCCACAAAAG CACGGCTGGC AGATAAAAAT
    AAGATTTATG AGTCCATTGA AGAAGCAAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG
    CTCTCAGAGG AAAGGACGTT AAAACAGAAG GTGGAGAATC TATTGCTGGA AGCTGAGAAA
    AGATGCTCTT TATTTGACTG TGACCTCAAG CAGTCACAGC AGAAAATAAA TGAACTCCTT
    AAACAGAAAG ATGTGCTAAA TGAGGATGTT AGAAACTTGA CATTAAAAAT AGAGCAGGAA
    ACTCAGAAGC GCTGCCTTAC ACAAAATGAC TTGAAGATGC AAACACAGCA AGTTAACACA
    CTAAAAATGT CAGAAAAACA GTTAAAACAA GAGAATAATC ATCTCATGGA AATGAAAATG
    AGCTTGGAAA AGCAGAATGC TGAACTTCGG AAAGAACGTC AAGATGCAGA TGGGCAAATG
    AAAGAACTTC AGGATCAGCT TGAAGCGGAG CAGTATTTCT CAACCCTCTA TAAAACACAG
    GTGAGGGAAC TTAAAGAAGA ATGTGAAGAA AAAACCAAAC TTTGTAAAGA ATTACAGCAA
    AAGAAACAGG AATTACAGGA AGAAAGGGAT TCCTTGGCTG CTCAACTGGA GATCACCTTG
    ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCAATTGCTG AAGAGCAGTA TTCTGATTTG
    GAAAAAGAGA AAATTATGAA GGAGCTTGAG ATCAAAGAGA TGATGGCTAG ACACAAACAG
    GAACTCACTG AAAAAGATGC TACAATTGCA TCTCTTGAAG AAACGAATAG AACACTAACT
    AGTGATGTTG CCAATCTTGC AAATGAGAAA GAAGAATTAA ATAACAAATT GAAAGATGCA
    CAAGAACAGC TGTCACGGCT GAAGGATGAG GAGATCAGTG CAGCTGCTAT TAAAGCACAG
    TTTGAGAAGC AGCTGCTCAC AGAGAGAACA CTGAAAACTC AAGCTGTGAA TAAGTTGGCT
    GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACTGATGT GCGGAGAAAA
    GAGAAAGAAA ATCGGAAGCT ACATATGGAG CTTAAATCTG AACGTGAAAA ACTGACCCAG
    CAGATGATCA AGTACCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC
    CAGATTCGAA TTGAACTGCA GATGACACTG GACAGTAAAG ATAGTGACAT TGAGCAGCTG
    CGGTCACAGC TCCAGGCTTT GCATATTGGT CTGGATAGTT CCAGTATAGG CAGTGGACCG
    GGGGACGCTG AGGCAGATGA TGGGTTTCCA GTTCATATCA CTCAATCACG CACTATGGAA
    TCCATGTCAT TCACCTACCA GCGTTCCTCT ACTTCTCTCA ATATTGCCAC TAAGCCTTCC
    AGTTCTCATA TGCTTCTTGA CTCTGATTCT GACTCAGAAG AAGAATCTTT GCCTTACTTA
    CCTATTTCAT CGGAACCTAA TGAATCAAGA CTAGAAGGAT GGCTTTCATT GCCTTTACGA
    AACAACACTA AGAAATTTGG ATGGACTAAA AAGTATGTGA TTGTAAGCAG TAAGAAGATT
    CTTTTCTATG ACAGCGAACA AGATAAAGAA CAATCTAATC CTTACATGGT TTTAGATATA
    GACAAATTAT TTCATGTCCG ACCAGTTACA CAGACAGATG TATATAGAGC AGATGCTAAA
    GATATTCCAA GGATATTTCA GATTCTGTAC GCCAATGAAG GAGAAAGTAA GAAGGAACAA
    GAATTTCCAG TGGAGCCAGT AGGAGAAAAA TCAAATTATA TTTGCCATAA AGGACATGAA
    TTTATTCCTA CTCTGTATCA TTTCCCAACC AACTGTGAGG CTTGTATGAA GCCATTGTGG
    CATATGTTTA AACCTCCTCC TGCTTTAGAA TGCCGGCGCT GCCATATTAA ATGTCATAAA
    GATCACATGG ACAAAAAGGA GGAGATTATA GCACCTTGCA AGGTTTATTG TGATATTTCG
    ACGGCAAAGA ATCTATTGTT GTTAGCAAAT TCTACAGAAG AGCAGCAGAA GTGGGTTAGT
    CGGTTGGTGA AAAAGATTCC TAAAAAGCCT CCAGCTCCAG ACCCTTTTGC ACGGTCATCA
    CCTAGAACTT CAATGAAGAT ACAACAAAAC CAGTCTATTA GACGGCCAAG TCGACAGCTT
    GCCCCAAACA AACCAAGATT GCTTGATTTG GCATTTAAAG ACTGGGATTG GTCATTTGAT
    GATGTTGATG GTGGTGATGA TGATGACGAT GTTTTTGATT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975706.1
    CDS1..5145
    Translation

    Target ORF information:

    RefSeq Version XM_014120231.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho-associated, coiled-coil containing protein kinase 2 (ROCK2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014120231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCATTCC ACACGGATGG AAACTTGCTT AACCAAAGAG CGAAGAAGAG AAGAGCCAAG 
    TATGTTCAGT TTGAGAAAAC CAGCATACCT GCCAGGACTC TTTCTGTTAA TGTGACAGAG
    CTTGCTCGGA TTATTCCCCA GTTGTTGGGA CAACTAGGCC CCTTCCCAGA TTGCGCCCTG
    CGAGGATCGG ACCAATGGCA CGAGCTGCGA GCCGGCAGGA ATGCGGAGGA GCTGCCCCGT
    AACCTGCCCT TCTTCCGCCC CCCAAAGGCA AGGAGGAGCT GGAGAGGGCC TGGGGACCGA
    GGCCTCTTCT ATCACCCTTT TATGCTCCTC CGCGGATGGG TGCTCAGGCC CGGGGCCGCG
    AGAAGACCGC TAGGGACCTG CACAAAGGCT CTCGCATCCC AGCACTTCTG CCCCGGGGGC
    GAAGCCGGGC GGGGAGTGCG GTGGCCGACC CCGCTGCGCG CCCCTCCGGC CCGTCCGCGG
    CCCTCCCCCC GCCCCGGGCC CAGGCAGGGG GCAGGGGCGC CCTGCAGCCT CCAGGCCAGC
    GGCATGCGGC GCCGGGCCGT GGGTCGGGGT CGGGGTCGGG GCCGGGGAAA CCCGCCGCGC
    GCCCTCTGGG GAGATGACAA AGAGCCGGGG CCAAGGAATC CGTCCGAGCT GTCGAGGAGA
    AGGGATCTCT CCTTCTCGCG GTCCGTCCTT CCTCAGGCAG CCCGTGGAAG ACCCCTGTCC
    CTAGTCAGCA CTCCGCGCGG GTTGGCCACG GGGCAGGACC TGGCTGAAGA CCGCCTCTCG
    GTGCAGGAGC GGGACGTGGT GATGGGCTGC GTCCCGTCCG ACGTCCTCGC CCTGGCGTCT
    CCCCGATTTT CGCGTCAGCG TGTGGATGGC TTAAATTCCT TGGTCCTTGA TTTGGATTTT
    CCTGCTTTGA GGAAAAACAA GAATATAGAT AATTTTTTAA ATAGATATGA GAAAATTGTG
    AAAAAAATCA GAGGTCTACA GATGAAGGCA GAAGACTATG ATGTTGTAAA AGTTATTGGA
    AGAGGTGCTT TTGGTGAGGT GCAGCTGGTT CGTCATAAAG CATCACAGAA GGTGTATGCT
    ATGAAGCTTC TTAGTAAGTT TGAAATGATA AAAAGATCAG ATTCTGCTTT TTTCTGGGAA
    GAGAGAGATA TTATGGCCTT TGCCAACAGT CCCTGGGTGG TTCAGCTCTT TTGTGCTTTT
    CAAGATGATA AGTATCTGTA CATGGTAATG GAGTACATGC CTGGTGGAGA TCTTGTAAAC
    CTTATGAGTA ACTATGACGT GCCTGAAAAA TGGGCCAAAT TCTATACTGC TGAAGTTGTT
    CTTGCTCTGG ATGCCATACA CTCCATGGGC TTAATTCACA GAGATGTAAA GCCTGACAAC
    ATGCTCTTGG ATAAACATGG ACATCTAAAG TTAGCAGATT TTGGCACATG TATGAAGATG
    GATGAAACAG GCATGGTGCA TTGTGATACA GCAGTTGGAA CACCTGATTA TATTTCACCT
    GAGGTTCTCA AATCACAAGG GGGTGATGGT TACTATGGGC GAGAATGTGA TTGGTGGTCG
    GTGGGTGTTT TCCTTTTTGA GATGCTGGTG GGGGATACTC CATTTTATGC AGATTCACTT
    GTAGGAACTT ATAGCAAAAT TATGGATCAT AAAAACTCAC TGTGTTTCCC TGAAGATGCA
    GAAATTTCTA AACATGCGAA GAATCTCATT TGCGCCTTCT TAACAGATAG GGAGGTACGA
    CTTGGGAGAA ATGGGGTAGA AGAAATCAAA CAGCATCCTT TCTTTAAGAA TGATCAATGG
    AATTGGGATA ACATAAGAGA GACGGCAGCT CCTGTAGTAC CAGAACTCAG CAGTGACATA
    GACAGCAGCA ATTTTGATGA CATCGAAGAT GATAAAGGAG ATGTAGAAAC TTTTCCAATT
    CCTAAAGCTT TTGTGGGAAA TCAGCTGCCT TTTATAGGAT TTACCTACTA TAGAGAAAAT
    CTGTTATTAA GTGAGTCTTC ATCTTGTAGA GAAAATGATT CATTACAATC AAGGAAAAAT
    GAAGAAAGTC AAGAGATTCA GAAAAAATTA TATACATTAG AAGAACACCT CAGCACTGAG
    ATACAAGCCA AAGAAGAACT AGAGCAAAAG TGCAAATCTG TTAATACTCG ACTTGAAAAA
    GTAGCAAAGG AATTAGAAGA AGAGATTACT TTAAGGAAAA ATGTGGAATC AGCATTAAGA
    CAATTAGAAA GAGAAAAGGC ACTTCTTCAG CACAAAAATG CAGAATATCA GCGGAAAGCT
    GATCACGAAG CAGACAAGAA GCGGAATTTG GAAAATGATG TTAACAGTTT AAAGGATCAA
    CTTGAAGATT TGAAAAAAAG AAATCAGAAC TCTCAAATAT CCACCGAGAA AGTGAATCAG
    CTCCAGAGAC AACTGGATGA AACCAATGCT TTACTGAGAA CAGAATCTGA TACTACGGCC
    CGGTTAAGAA AAACCCAGGC AGAAAGTTCA AAACAGATTC AGCAGCTGGA ATCTAACAAT
    AGAGATCTCC AAGATAAAAA TTGCCTGCTA GAGACTGCCA AGTTAAAACT TGAAAAGGAA
    TTTATGAATC TTCAGTCAGT TCTAGAATCT GAAAGGAGGG ACCGAACCCA CGGATCCGAG
    ATAATTAATG ACTTACAAGG TAGAATATCT GGCCTAGAAG AAGACTTAAA GAATGGAAAA
    ATCTTATTAG CAAAAGCAGA GGTGGAGAAG AGACAACTGC AGGAGAGACT TACTGATTTG
    GAAAAGGAAA AGAACAACAT GGAAATAGAT ATGACGTACC AACTAAAAGT TATACAGCAG
    AGCTTAGAAC AAGAAGAAGC TGAGCATAAG GCCACAAAAG CACGGCTGGC AGATAAAAAT
    AAGATTTATG AGTCCATTGA AGAAGCAAAA TCAGAAGCCA TGAAAGAAAT GGAGAAGAAG
    CTCTCAGAGG AAAGGACGTT AAAACAGAAG GTGGAGAATC TATTGCTGGA AGCTGAGAAA
    AGATGCTCTT TATTTGACTG TGACCTCAAG CAGTCACAGC AGAAAATAAA TGAACTCCTT
    AAACAGAAAG ATGTGCTAAA TGAGGATGTT AGAAACTTGA CATTAAAAAT AGAGCAGGAA
    ACTCAGAAGC GCTGCCTTAC ACAAAATGAC TTGAAGATGC AAACACAGCA AGTTAACACA
    CTAAAAATGT CAGAAAAACA GTTAAAACAA GAGAATAATC ATCTCATGGA AATGAAAATG
    AGCTTGGAAA AGCAGAATGC TGAACTTCGG AAAGAACGTC AAGATGCAGA TGGGCAAATG
    AAAGAACTTC AGGATCAGCT TGAAGCGGAG CAGTATTTCT CAACCCTCTA TAAAACACAG
    GTGAGGGAAC TTAAAGAAGA ATGTGAAGAA AAAACCAAAC TTTGTAAAGA ATTACAGCAA
    AAGAAACAGG AATTACAGGA AGAAAGGGAT TCCTTGGCTG CTCAACTGGA GATCACCTTG
    ACCAAAGCAG ATTCTGAGCA GCTGGCTCGT TCAATTGCTG AAGAGCAGTA TTCTGATTTG
    GAAAAAGAGA AAATTATGAA GGAGCTTGAG ATCAAAGAGA TGATGGCTAG ACACAAACAG
    GAACTCACTG AAAAAGATGC TACAATTGCA TCTCTTGAAG AAACGAATAG AACACTAACT
    AGTGATGTTG CCAATCTTGC AAATGAGAAA GAAGAATTAA ATAACAAATT GAAAGATGCA
    CAAGAACAGC TGTCACGGCT GAAGGATGAG GAGATCAGTG CAGCTGCTAT TAAAGCACAG
    TTTGAGAAGC AGCTGCTCAC AGAGAGAACA CTGAAAACTC AAGCTGTGAA TAAGTTGGCT
    GAGATCATGA ATCGAAAAGA ACCTGTCAAG CGTGGTAGTG ACACTGATGT GCGGAGAAAA
    GAGAAAGAAA ATCGGAAGCT ACATATGGAG CTTAAATCTG AACGTGAAAA ACTGACCCAG
    CAGATGATCA AGTACCAGAA AGAACTGAAT GAAATGCAGG CTCAAATAGC TGAAGAGAGC
    CAGATTCGAA TTGAACTGCA GATGACACTG GACAGTAAAG ATAGTGACAT TGAGCAGCTG
    CGGTCACAGC TCCAGGCTTT GCATATTGGT CTGGATAGTT CCAGTATAGG CAGTGGACCG
    GGGGACGCTG AGGCAGATGA TGGGTTTCCA GTTCATATCA CTCAATCACG CACTATGGAA
    TCCATGTCAT TCACCTACCA GCGTTCCTCT ACTTCTCTCA ATATTGCCAC TAAGCCTTCC
    AGTTCTCATA TGCTTCTTGA CTCTGATTCT GACTCAGAAG AAGAATCTTT GCCTTACTTA
    CCTATTTCAT CGGAACCTAA TGAATCAAGA CTAGAAGGAT GGCTTTCATT GCCTTTACGA
    AACAACACTA AGAAATTTGG ATGGACTAAA AAGTATGTGA TTGTAAGCAG TAAGAAGATT
    CTTTTCTATG ACAGCGAACA AGATAAAGAA CAATCTAATC CTTACATGGT TTTAGATATA
    GACAAATTAT TTCATGTCCG ACCAGTTACA CAGACAGATG TATATAGAGC AGATGCTAAA
    GATATTCCAA GGATATTTCA GATTCTGTAC GCCAATGAAG GAGAAAGTAA GAAGGAACAA
    GAATTTCCAG TGGAGCCAGT AGGAGAAAAA TCAAATTATA TTTGCCATAA AGGACATGAA
    TTTATTCCTA CTCTGTATCA TTTCCCAACC AACTGTGAGG CTTGTATGAA GCCATTGTGG
    CATATGTTTA AACCTCCTCC TGCTTTAGAA TGCCGGCGCT GCCATATTAA ATGTCATAAA
    GATCACATGG ACAAAAAGGA GGAGATTATA GCACCTTGCA AGGTTTATTG TGATATTTCG
    ACGGCAAAGA ATCTATTGTT GTTAGCAAAT TCTACAGAAG AGCAGCAGAA GTGGGTTAGT
    CGGTTGGTGA AAAAGATTCC TAAAAAGCCT CCAGCTCCAG ACCCTTTTGC ACGGTCATCA
    CCTAGAACTT CAATGAAGAT ACAACAAAAC CAGTCTATTA GACGGCCAAG TCGACAGCTT
    GCCCCAAACA AACCAAGATT GCTTGATTTG GCATTTAAAG ACTGGGATTG GTCATTTGAT
    GATGTTGATG GTGGTGATGA TGATGACGAT GTTTTTGATT TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.