UBE3C cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE3C
Entrez Gene ID	482822
Full Name	ubiquitin protein ligase E3C
General protein information	Preferred Names ubiquitin protein ligase E3C Names ubiquitin-protein ligase E3C
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014119888.1	XP_013975363.1	ubiquitin-protein ligase E3C isoform X3
XM_539937.6	XP_539937.2	ubiquitin-protein ligase E3C isoform X1
XM_022403920.1	XP_022259628.1	ubiquitin-protein ligase E3C isoform X5
XM_539937.5	XP_539937.2	ubiquitin-protein ligase E3C isoform X1
XM_014119887.2	XP_013975362.1	ubiquitin-protein ligase E3C isoform X2
XM_014119888.2	XP_013975363.1	ubiquitin-protein ligase E3C isoform X3
XM_014119887.1	XP_013975362.1	ubiquitin-protein ligase E3C isoform X2
XM_022403919.1	XP_022259627.1	ubiquitin-protein ligase E3C isoform X4

Pathways from BioSystems

Homologies

	UBE3C	XP_002803585.1
Canis lupus familiaris (dog)	UBE3C	XP_539937.2
Pan troglodytes (chimpanzee)	UBE3C	XP_528010.3
Drosophila melanogaster (fruit fly)	CG3356	NP_611896.1
Gallus gallus (chicken)	UBE3C	NP_001026138.1
Bos taurus (cattle)	UBE3C	NP_001179117.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011474	XP_317832.4
Homo sapiens (human)	UBE3C	NP_055486.2
Mus musculus (house mouse)	Ube3c	NP_598668.1
Rattus norvegicus (Norway rat)	Ube3c	XP_006235923.1
Danio rerio (zebrafish)	ube3c	NP_956767.1
Xenopus tropicalis (tropical clawed frog)	ube3c	NP_001072518.1

Gene Ontology

The following UBE3C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE3C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe293929	XM_022403920.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe293928	XM_022403919.1 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe40465	XM_014119887.2 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe18146	XM_539937.6 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe18147	XM_014119888.2 Latest version!	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe18146	XM_539937.5	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe18147	XM_014119888.1	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe40465	XM_014119887.1	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE3C ( XM_022403920.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259628.1 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe293929
Clone ID Related Accession (Same CDS sequence)	XM_022403920.1
Accession Version	XM_022403920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin-protein ligase E3C isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCCATGAGGA CCCGAGGGCA 
GGGAAACAAG GTGACAAGAC AAGATATGGT GTTGTGGTTA GAAGGGGGCC ACGTGGCCTG 
ACAGGAAGGA GTCAGAGTGT GGGGTGGTTG AAATCAGATC AGGGAGGAGC ACTCAGTGAC 
TGGCAGCAGC ACTTCCAGGA CAGGCCTAAA GACTGGGTGG CTGAGGCGGA GCAGAGGACG 
GCTGAGGGCA GAGTGACAGA AATGTGTGGG AGCCAGCTAC AGGCTCAGGG TCAGCGTCAG 
AGCATGTGGG AGACGGCTAC GGAGTCGGGG TCAGGGAGCT GGCAGCCATG GGAAGAATGG 
CAGTGGGTTT TCTGGCCCAG TACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259628.1
CDS	69..2915
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLIWMYQNLIKHSSLFVQQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALHEDPRAGKQGDKTRYGVVVRRGPRGLTGRSQSVGWLKSDQGGALSDWQQHFQDRPKDWVAEAEQRTAEGRVTEMCGSQLQAQGQRQSMWETATESGSGSWQPWEEWQWVFWPST

Target ORF information:

RefSeq Version	XM_022403920.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403920.1

ORF Insert Sequence:

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCCATGAGGA CCCGAGGGCA 
GGGAAACAAG GTGACAAGAC AAGATATGGT GTTGTGGTTA GAAGGGGGCC ACGTGGCCTG 
ACAGGAAGGA GTCAGAGTGT GGGGTGGTTG AAATCAGATC AGGGAGGAGC ACTCAGTGAC 
TGGCAGCAGC ACTTCCAGGA CAGGCCTAAA GACTGGGTGG CTGAGGCGGA GCAGAGGACG 
GCTGAGGGCA GAGTGACAGA AATGTGTGGG AGCCAGCTAC AGGCTCAGGG TCAGCGTCAG 
AGCATGTGGG AGACGGCTAC GGAGTCGGGG TCAGGGAGCT GGCAGCCATG GGAAGAATGG 
CAGTGGGTTT TCTGGCCCAG TACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_022403919.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259627.1 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe293928
Clone ID Related Accession (Same CDS sequence)	XM_022403919.1
Accession Version	XM_022403919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin-protein ligase E3C isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCTATGAAAA TATGCTGGTA 
GAATTGCCCT TTGCTGGCTT TTTCCTGTCC AAATTGCTTG GAACCAGTGC AGATGTGGAC 
ATTCACCATC TGGCCTCGTT AGATCCTGAA GTGTATAAGA ATCTGCTTTT TCTCAAGAGC 
TATGAAGGTG ACGTGGAGGA GCTTGGACTG AACTTCACCG TGGTGAACAA TGACCTGGGG 
GAAGCACAGG TAGTAGAACT AAAATTTGGT GGAAAAGACA TCCCTGTCAC CAGTGCCAAC 
AGGATCGCGT ACATCCACCT TGTTGCTGAC TACAGATTGA ACAGACAGAT CCGCCAGCAC 
TGCCTGGCCT TCAGACAGGG CCTGGCCAAT GTGGTGAATC TAGAGTGGCT GCGAATGTTT 
GATCAGCAGG AGATTCAGGT ATTAATTTCT GGTGCACAAG TTCCCATAAG TCTGGAGGAC 
CTAAAATCCT TTACAAATTA TTCAGGAGGC TATTCTGCTG ACCATCCTGT AATCAAAGTC 
TTCTGGAGAG TTGTGGAAGG GTTCACGGAT GAAGAAAAGC GTAAGCTGCT CAAGTTTGTG 
ACCAGCTGCT CCCGACCCCC TCTCCTGGGC TTTAAGGATA CAATCCTGGA AGTAGAGATC 
AGAAGACATG CATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259627.1
CDS	69..3206
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLIWMYQNLIKHSSLFVQQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKDTILEVEIRRHAW

Target ORF information:

RefSeq Version	XM_022403919.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403919.1

ORF Insert Sequence:

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCTATGAAAA TATGCTGGTA 
GAATTGCCCT TTGCTGGCTT TTTCCTGTCC AAATTGCTTG GAACCAGTGC AGATGTGGAC 
ATTCACCATC TGGCCTCGTT AGATCCTGAA GTGTATAAGA ATCTGCTTTT TCTCAAGAGC 
TATGAAGGTG ACGTGGAGGA GCTTGGACTG AACTTCACCG TGGTGAACAA TGACCTGGGG 
GAAGCACAGG TAGTAGAACT AAAATTTGGT GGAAAAGACA TCCCTGTCAC CAGTGCCAAC 
AGGATCGCGT ACATCCACCT TGTTGCTGAC TACAGATTGA ACAGACAGAT CCGCCAGCAC 
TGCCTGGCCT TCAGACAGGG CCTGGCCAAT GTGGTGAATC TAGAGTGGCT GCGAATGTTT 
GATCAGCAGG AGATTCAGGT ATTAATTTCT GGTGCACAAG TTCCCATAAG TCTGGAGGAC 
CTAAAATCCT TTACAAATTA TTCAGGAGGC TATTCTGCTG ACCATCCTGT AATCAAAGTC 
TTCTGGAGAG TTGTGGAAGG GTTCACGGAT GAAGAAAAGC GTAAGCTGCT CAAGTTTGTG 
ACCAGCTGCT CCCGACCCCC TCTCCTGGGC TTTAAGGATA CAATCCTGGA AGTAGAGATC 
AGAAGACATG CATGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_014119887.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975362.1 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe40465
Clone ID Related Accession (Same CDS sequence)	XM_014119887.2 , XM_014119887.1
Accession Version	XM_014119887.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin-protein ligase E3C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGCAGTTGGA TGGATCTGAG 
AGACTTACGT GTTTATTTCA AATAAAGAGG TTGATGAGCC TCTGTTGCAG GTTATTGCAA 
AACTGTAATG ATGACAGTTT GAATGTTGCA CTTCCAATGA GGATGCTTGA GGTGTTTTCA 
TCTGAGAATA CTTATTTGCC TGTTTTACAT GACGCTAGCT ATGTGGTGTC CATAATTGAA 
CAGATTTTGC ACTACATGAT TCAAAATGGG TATTATAGAT CTCTCTATTT GTTAATTAAC 
AGCAAGCTTC CATCAAGTAT TGAGTATTCT GATTTATCTC GAGTTCCTAT AGCAAACATT 
TTGCTAGAGA ATGTTCTGAA ACCATTGCAC TTTACTTACA ACTCCTGTCC AGAAGGTGCA 
AGGCAGCAAG TTTTTAGCGC CTTCACTGAG GAGTTTTTGG CAGCACCTTT TACAGATCAG 
ATTTTTCATT TCATCATTCC AGCCTTAGCG GATGCACACA CTGTTTTCCC TTACGAGCCC 
TTCCTGAGCG CGCTGTTGTT ACTGGAGAGC AGACGCTCCG GGACGAGCAG CAGGACAAGC 
AGCAGGGCAC CGTGGCTCTT CTTCTTTGTC TTAACTGTTG GCGAGCAGTA TCTAGGGACC 
CTCTCTGAGG AAGGACTGCT GGTGTATTTA AGAGTACTCC AGACCTTTCT TTCTCAGCTA 
CCAGTTTCAC CGGCCAGCAC GAGCTGTCAG GATTCAGCCA GTGACTCGGA AGATGAGGGT 
GAAGAGGCAG ACAAGCAGCC AAGCGCTCCT GAGGATGGTC GACTGTCAGT CCCATACATA 
ACAGAGGAGT GTCTGAAGAA GTTGGACACA AAGCAGCAGA CCAACACTCT GCTAAACCTG 
GTATGGAGGG ACTCGGCCAG TGAGGAGGCT TTGACTCTAA TGGCGTCGAT TGGCCATACA 
CTCATGGTGC AGCATCGGAT GGTGGTGCCC AAAGTCAGGC TTCTCTACAG TCTAGCCTTT 
AATGCCAGGT TTCTGAGACA TCTTTGGTTT CTCATTTCTT CCATGACAAC ACGGATGATC 
ACAGGGTCTG TGGTGCCACT GCTCCAGGTG ATCTCGAGGG GCTCCCCCAT GTCTTTGGAA 
GACTCCAGTC GGATCATCCC TCTCTTCTAC TTGTTTAGCT CTCTGTTCAG TCACTCGCTA 
ATTTCCATAC ATGACAATGA ATTCTTTGGA GATCCCATAG AAGTTGTAGG TCAAAGACAA 
TCATCAATGA TGCCTTTTAC TCTAGAAGAA CTAATAATGT TGTCTCGCTG CCTTCGAGAT 
GCATGCTTAG GGATCATCAA GTTGGCTTAT CCAGAAACAA AACCAGAAGT TCGAGAAGAA 
TATATTACAG CATTTCAAAG TATTGGAGTT ACGACTAATT CTGAAATGCA GCAGTGTATA 
CAGATGGAAC AGAAACGATG GATTCAATTA TTTAAGGTGA TTACCAATCT AGTGAAAATG 
CTCAAGTCCA GAGACACAAG GAGAAATTTT TGTCCTCCAA ATCACTGGCT GTCAGAACAA 
GAAGATATTA AAGCAGATAA GGTCACCCAG CTCTACGTCC CGGCTTCCAG ACACGTGTGG 
AGGTTCCGGC GGATGGGGAG AATTGGCCCC CTGCAGTCGA GCCTGGACGT GGGTTTGGAG 
TCCCCGCCAC TGTCTGTGTC TGAGGAACGA CAGCTCGCCA TCCTGACAGA GCTGCCCTTT 
GTGGTTCCAT TTGAGGAGCG AGTCAAGATC TTTCAAAGGT TGATTTATGC AGATAAGCAA 
GAAGTTCAAG GAGATGGCCC ATTCCTGGAT GGAATTAATG TCACAATACG AAGAAATTAT 
ATTTATGAAG ATGCTTATGA CAAACTTTCC CCAGAAAATG AGCCTGATTT GAAAAAGCGG 
ATCCGTGTGC ACTTACTCAA TGCCCATGGC CTGGATGAAG CTGGCATTGA TGGTGGTGGC 
ATTTTCAGAG AGTTTCTGAA TGAGCTGCTC AAGTCAGGAT TTAACCCCAA CCAGGGGTTC 
TTTAAGACCA CGAATGAAGG GCTTCTGTAC CCTAGCCCAG CCGCTCAGAT GCTCGTGGGA 
GATTCCTTTG CCAGACACTA CTACTTCCTG GGCAGGATGC TTGGAAAGGC TCTCTATGAA 
AATATGCTGG TAGAATTGCC CTTTGCTGGC TTTTTCCTGT CCAAATTGCT TGGAACCAGT 
GCAGATGTGG ACATTCACCA TCTGGCCTCG TTAGATCCTG AAGTGTATAA GAATCTGCTT 
TTTCTCAAGA GCTATGAAGG TGACGTGGAG GAGCTTGGAC TGAACTTCAC CGTGGTGAAC 
AATGACCTGG GGGAAGCACA GGTAGTAGAA CTAAAATTTG GTGGAAAAGA CATCCCTGTC 
ACCAGTGCCA ACAGGATCGC GTACATCCAC CTTGTTGCTG ACTACAGATT GAACAGACAG 
ATCCGCCAGC ACTGCCTGGC CTTCAGACAG GGCCTGGCCA ATGTGGTGAA TCTAGAGTGG 
CTGCGAATGT TTGATCAGCA GGAGATTCAG GTATTAATTT CTGGTGCACA AGTTCCCATA 
AGTCTGGAGG ACCTAAAATC CTTTACAAAT TATTCAGGAG GCTATTCTGC TGACCATCCT 
GTAATCAAAG TCTTCTGGAG AGTTGTGGAA GGGTTCACGG ATGAAGAAAA GCGTAAGCTG 
CTCAAGTTTG TGACCAGCTG CTCCCGACCC CCTCTCCTGG GCTTTAAGGA GCTGTACCCC 
GCATTCTGCA TTCACAACGG GGGCTCTGAC CTTGAGCGGC TCCCCACGGC CAGCACCTGC 
ATGAACCTGC TGAAGCTTCC TGAGTTTTAC GACGAGACCC TTCTGCGGAG TAAGCTCCTC 
TATGCTATCG AATGTGCTGC TGGCTTTGAG CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975362.1
CDS	69..3287
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_014119887.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119887.2

ORF Insert Sequence:

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGCAGTTGGA TGGATCTGAG 
AGACTTACGT GTTTATTTCA AATAAAGAGG TTGATGAGCC TCTGTTGCAG GTTATTGCAA 
AACTGTAATG ATGACAGTTT GAATGTTGCA CTTCCAATGA GGATGCTTGA GGTGTTTTCA 
TCTGAGAATA CTTATTTGCC TGTTTTACAT GACGCTAGCT ATGTGGTGTC CATAATTGAA 
CAGATTTTGC ACTACATGAT TCAAAATGGG TATTATAGAT CTCTCTATTT GTTAATTAAC 
AGCAAGCTTC CATCAAGTAT TGAGTATTCT GATTTATCTC GAGTTCCTAT AGCAAACATT 
TTGCTAGAGA ATGTTCTGAA ACCATTGCAC TTTACTTACA ACTCCTGTCC AGAAGGTGCA 
AGGCAGCAAG TTTTTAGCGC CTTCACTGAG GAGTTTTTGG CAGCACCTTT TACAGATCAG 
ATTTTTCATT TCATCATTCC AGCCTTAGCG GATGCACACA CTGTTTTCCC TTACGAGCCC 
TTCCTGAGCG CGCTGTTGTT ACTGGAGAGC AGACGCTCCG GGACGAGCAG CAGGACAAGC 
AGCAGGGCAC CGTGGCTCTT CTTCTTTGTC TTAACTGTTG GCGAGCAGTA TCTAGGGACC 
CTCTCTGAGG AAGGACTGCT GGTGTATTTA AGAGTACTCC AGACCTTTCT TTCTCAGCTA 
CCAGTTTCAC CGGCCAGCAC GAGCTGTCAG GATTCAGCCA GTGACTCGGA AGATGAGGGT 
GAAGAGGCAG ACAAGCAGCC AAGCGCTCCT GAGGATGGTC GACTGTCAGT CCCATACATA 
ACAGAGGAGT GTCTGAAGAA GTTGGACACA AAGCAGCAGA CCAACACTCT GCTAAACCTG 
GTATGGAGGG ACTCGGCCAG TGAGGAGGCT TTGACTCTAA TGGCGTCGAT TGGCCATACA 
CTCATGGTGC AGCATCGGAT GGTGGTGCCC AAAGTCAGGC TTCTCTACAG TCTAGCCTTT 
AATGCCAGGT TTCTGAGACA TCTTTGGTTT CTCATTTCTT CCATGACAAC ACGGATGATC 
ACAGGGTCTG TGGTGCCACT GCTCCAGGTG ATCTCGAGGG GCTCCCCCAT GTCTTTGGAA 
GACTCCAGTC GGATCATCCC TCTCTTCTAC TTGTTTAGCT CTCTGTTCAG TCACTCGCTA 
ATTTCCATAC ATGACAATGA ATTCTTTGGA GATCCCATAG AAGTTGTAGG TCAAAGACAA 
TCATCAATGA TGCCTTTTAC TCTAGAAGAA CTAATAATGT TGTCTCGCTG CCTTCGAGAT 
GCATGCTTAG GGATCATCAA GTTGGCTTAT CCAGAAACAA AACCAGAAGT TCGAGAAGAA 
TATATTACAG CATTTCAAAG TATTGGAGTT ACGACTAATT CTGAAATGCA GCAGTGTATA 
CAGATGGAAC AGAAACGATG GATTCAATTA TTTAAGGTGA TTACCAATCT AGTGAAAATG 
CTCAAGTCCA GAGACACAAG GAGAAATTTT TGTCCTCCAA ATCACTGGCT GTCAGAACAA 
GAAGATATTA AAGCAGATAA GGTCACCCAG CTCTACGTCC CGGCTTCCAG ACACGTGTGG 
AGGTTCCGGC GGATGGGGAG AATTGGCCCC CTGCAGTCGA GCCTGGACGT GGGTTTGGAG 
TCCCCGCCAC TGTCTGTGTC TGAGGAACGA CAGCTCGCCA TCCTGACAGA GCTGCCCTTT 
GTGGTTCCAT TTGAGGAGCG AGTCAAGATC TTTCAAAGGT TGATTTATGC AGATAAGCAA 
GAAGTTCAAG GAGATGGCCC ATTCCTGGAT GGAATTAATG TCACAATACG AAGAAATTAT 
ATTTATGAAG ATGCTTATGA CAAACTTTCC CCAGAAAATG AGCCTGATTT GAAAAAGCGG 
ATCCGTGTGC ACTTACTCAA TGCCCATGGC CTGGATGAAG CTGGCATTGA TGGTGGTGGC 
ATTTTCAGAG AGTTTCTGAA TGAGCTGCTC AAGTCAGGAT TTAACCCCAA CCAGGGGTTC 
TTTAAGACCA CGAATGAAGG GCTTCTGTAC CCTAGCCCAG CCGCTCAGAT GCTCGTGGGA 
GATTCCTTTG CCAGACACTA CTACTTCCTG GGCAGGATGC TTGGAAAGGC TCTCTATGAA 
AATATGCTGG TAGAATTGCC CTTTGCTGGC TTTTTCCTGT CCAAATTGCT TGGAACCAGT 
GCAGATGTGG ACATTCACCA TCTGGCCTCG TTAGATCCTG AAGTGTATAA GAATCTGCTT 
TTTCTCAAGA GCTATGAAGG TGACGTGGAG GAGCTTGGAC TGAACTTCAC CGTGGTGAAC 
AATGACCTGG GGGAAGCACA GGTAGTAGAA CTAAAATTTG GTGGAAAAGA CATCCCTGTC 
ACCAGTGCCA ACAGGATCGC GTACATCCAC CTTGTTGCTG ACTACAGATT GAACAGACAG 
ATCCGCCAGC ACTGCCTGGC CTTCAGACAG GGCCTGGCCA ATGTGGTGAA TCTAGAGTGG 
CTGCGAATGT TTGATCAGCA GGAGATTCAG GTATTAATTT CTGGTGCACA AGTTCCCATA 
AGTCTGGAGG ACCTAAAATC CTTTACAAAT TATTCAGGAG GCTATTCTGC TGACCATCCT 
GTAATCAAAG TCTTCTGGAG AGTTGTGGAA GGGTTCACGG ATGAAGAAAA GCGTAAGCTG 
CTCAAGTTTG TGACCAGCTG CTCCCGACCC CCTCTCCTGG GCTTTAAGGA GCTGTACCCC 
GCATTCTGCA TTCACAACGG GGGCTCTGAC CTTGAGCGGC TCCCCACGGC CAGCACCTGC 
ATGAACCTGC TGAAGCTTCC TGAGTTTTAC GACGAGACCC TTCTGCGGAG TAAGCTCCTC 
TATGCTATCG AATGTGCTGC TGGCTTTGAG CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_014119887.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975362.1 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe40465
Clone ID Related Accession (Same CDS sequence)	XM_014119887.2 , XM_014119887.1
Accession Version	XM_014119887.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin-protein ligase E3C isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGCAGTTGGA TGGATCTGAG 
AGACTTACGT GTTTATTTCA AATAAAGAGG TTGATGAGCC TCTGTTGCAG GTTATTGCAA 
AACTGTAATG ATGACAGTTT GAATGTTGCA CTTCCAATGA GGATGCTTGA GGTGTTTTCA 
TCTGAGAATA CTTATTTGCC TGTTTTACAT GACGCTAGCT ATGTGGTGTC CATAATTGAA 
CAGATTTTGC ACTACATGAT TCAAAATGGG TATTATAGAT CTCTCTATTT GTTAATTAAC 
AGCAAGCTTC CATCAAGTAT TGAGTATTCT GATTTATCTC GAGTTCCTAT AGCAAACATT 
TTGCTAGAGA ATGTTCTGAA ACCATTGCAC TTTACTTACA ACTCCTGTCC AGAAGGTGCA 
AGGCAGCAAG TTTTTAGCGC CTTCACTGAG GAGTTTTTGG CAGCACCTTT TACAGATCAG 
ATTTTTCATT TCATCATTCC AGCCTTAGCG GATGCACACA CTGTTTTCCC TTACGAGCCC 
TTCCTGAGCG CGCTGTTGTT ACTGGAGAGC AGACGCTCCG GGACGAGCAG CAGGACAAGC 
AGCAGGGCAC CGTGGCTCTT CTTCTTTGTC TTAACTGTTG GCGAGCAGTA TCTAGGGACC 
CTCTCTGAGG AAGGACTGCT GGTGTATTTA AGAGTACTCC AGACCTTTCT TTCTCAGCTA 
CCAGTTTCAC CGGCCAGCAC GAGCTGTCAG GATTCAGCCA GTGACTCGGA AGATGAGGGT 
GAAGAGGCAG ACAAGCAGCC AAGCGCTCCT GAGGATGGTC GACTGTCAGT CCCATACATA 
ACAGAGGAGT GTCTGAAGAA GTTGGACACA AAGCAGCAGA CCAACACTCT GCTAAACCTG 
GTATGGAGGG ACTCGGCCAG TGAGGAGGCT TTGACTCTAA TGGCGTCGAT TGGCCATACA 
CTCATGGTGC AGCATCGGAT GGTGGTGCCC AAAGTCAGGC TTCTCTACAG TCTAGCCTTT 
AATGCCAGGT TTCTGAGACA TCTTTGGTTT CTCATTTCTT CCATGACAAC ACGGATGATC 
ACAGGGTCTG TGGTGCCACT GCTCCAGGTG ATCTCGAGGG GCTCCCCCAT GTCTTTGGAA 
GACTCCAGTC GGATCATCCC TCTCTTCTAC TTGTTTAGCT CTCTGTTCAG TCACTCGCTA 
ATTTCCATAC ATGACAATGA ATTCTTTGGA GATCCCATAG AAGTTGTAGG TCAAAGACAA 
TCATCAATGA TGCCTTTTAC TCTAGAAGAA CTAATAATGT TGTCTCGCTG CCTTCGAGAT 
GCATGCTTAG GGATCATCAA GTTGGCTTAT CCAGAAACAA AACCAGAAGT TCGAGAAGAA 
TATATTACAG CATTTCAAAG TATTGGAGTT ACGACTAATT CTGAAATGCA GCAGTGTATA 
CAGATGGAAC AGAAACGATG GATTCAATTA TTTAAGGTGA TTACCAATCT AGTGAAAATG 
CTCAAGTCCA GAGACACAAG GAGAAATTTT TGTCCTCCAA ATCACTGGCT GTCAGAACAA 
GAAGATATTA AAGCAGATAA GGTCACCCAG CTCTACGTCC CGGCTTCCAG ACACGTGTGG 
AGGTTCCGGC GGATGGGGAG AATTGGCCCC CTGCAGTCGA GCCTGGACGT GGGTTTGGAG 
TCCCCGCCAC TGTCTGTGTC TGAGGAACGA CAGCTCGCCA TCCTGACAGA GCTGCCCTTT 
GTGGTTCCAT TTGAGGAGCG AGTCAAGATC TTTCAAAGGT TGATTTATGC AGATAAGCAA 
GAAGTTCAAG GAGATGGCCC ATTCCTGGAT GGAATTAATG TCACAATACG AAGAAATTAT 
ATTTATGAAG ATGCTTATGA CAAACTTTCC CCAGAAAATG AGCCTGATTT GAAAAAGCGG 
ATCCGTGTGC ACTTACTCAA TGCCCATGGC CTGGATGAAG CTGGCATTGA TGGTGGTGGC 
ATTTTCAGAG AGTTTCTGAA TGAGCTGCTC AAGTCAGGAT TTAACCCCAA CCAGGGGTTC 
TTTAAGACCA CGAATGAAGG GCTTCTGTAC CCTAGCCCAG CCGCTCAGAT GCTCGTGGGA 
GATTCCTTTG CCAGACACTA CTACTTCCTG GGCAGGATGC TTGGAAAGGC TCTCTATGAA 
AATATGCTGG TAGAATTGCC CTTTGCTGGC TTTTTCCTGT CCAAATTGCT TGGAACCAGT 
GCAGATGTGG ACATTCACCA TCTGGCCTCG TTAGATCCTG AAGTGTATAA GAATCTGCTT 
TTTCTCAAGA GCTATGAAGG TGACGTGGAG GAGCTTGGAC TGAACTTCAC CGTGGTGAAC 
AATGACCTGG GGGAAGCACA GGTAGTAGAA CTAAAATTTG GTGGAAAAGA CATCCCTGTC 
ACCAGTGCCA ACAGGATCGC GTACATCCAC CTTGTTGCTG ACTACAGATT GAACAGACAG 
ATCCGCCAGC ACTGCCTGGC CTTCAGACAG GGCCTGGCCA ATGTGGTGAA TCTAGAGTGG 
CTGCGAATGT TTGATCAGCA GGAGATTCAG GTATTAATTT CTGGTGCACA AGTTCCCATA 
AGTCTGGAGG ACCTAAAATC CTTTACAAAT TATTCAGGAG GCTATTCTGC TGACCATCCT 
GTAATCAAAG TCTTCTGGAG AGTTGTGGAA GGGTTCACGG ATGAAGAAAA GCGTAAGCTG 
CTCAAGTTTG TGACCAGCTG CTCCCGACCC CCTCTCCTGG GCTTTAAGGA GCTGTACCCC 
GCATTCTGCA TTCACAACGG GGGCTCTGAC CTTGAGCGGC TCCCCACGGC CAGCACCTGC 
ATGAACCTGC TGAAGCTTCC TGAGTTTTAC GACGAGACCC TTCTGCGGAG TAAGCTCCTC 
TATGCTATCG AATGTGCTGC TGGCTTTGAG CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975362.1
CDS	69..3287
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_014119887.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119887.1

ORF Insert Sequence:

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGCAGTTGGA TGGATCTGAG 
AGACTTACGT GTTTATTTCA AATAAAGAGG TTGATGAGCC TCTGTTGCAG GTTATTGCAA 
AACTGTAATG ATGACAGTTT GAATGTTGCA CTTCCAATGA GGATGCTTGA GGTGTTTTCA 
TCTGAGAATA CTTATTTGCC TGTTTTACAT GACGCTAGCT ATGTGGTGTC CATAATTGAA 
CAGATTTTGC ACTACATGAT TCAAAATGGG TATTATAGAT CTCTCTATTT GTTAATTAAC 
AGCAAGCTTC CATCAAGTAT TGAGTATTCT GATTTATCTC GAGTTCCTAT AGCAAACATT 
TTGCTAGAGA ATGTTCTGAA ACCATTGCAC TTTACTTACA ACTCCTGTCC AGAAGGTGCA 
AGGCAGCAAG TTTTTAGCGC CTTCACTGAG GAGTTTTTGG CAGCACCTTT TACAGATCAG 
ATTTTTCATT TCATCATTCC AGCCTTAGCG GATGCACACA CTGTTTTCCC TTACGAGCCC 
TTCCTGAGCG CGCTGTTGTT ACTGGAGAGC AGACGCTCCG GGACGAGCAG CAGGACAAGC 
AGCAGGGCAC CGTGGCTCTT CTTCTTTGTC TTAACTGTTG GCGAGCAGTA TCTAGGGACC 
CTCTCTGAGG AAGGACTGCT GGTGTATTTA AGAGTACTCC AGACCTTTCT TTCTCAGCTA 
CCAGTTTCAC CGGCCAGCAC GAGCTGTCAG GATTCAGCCA GTGACTCGGA AGATGAGGGT 
GAAGAGGCAG ACAAGCAGCC AAGCGCTCCT GAGGATGGTC GACTGTCAGT CCCATACATA 
ACAGAGGAGT GTCTGAAGAA GTTGGACACA AAGCAGCAGA CCAACACTCT GCTAAACCTG 
GTATGGAGGG ACTCGGCCAG TGAGGAGGCT TTGACTCTAA TGGCGTCGAT TGGCCATACA 
CTCATGGTGC AGCATCGGAT GGTGGTGCCC AAAGTCAGGC TTCTCTACAG TCTAGCCTTT 
AATGCCAGGT TTCTGAGACA TCTTTGGTTT CTCATTTCTT CCATGACAAC ACGGATGATC 
ACAGGGTCTG TGGTGCCACT GCTCCAGGTG ATCTCGAGGG GCTCCCCCAT GTCTTTGGAA 
GACTCCAGTC GGATCATCCC TCTCTTCTAC TTGTTTAGCT CTCTGTTCAG TCACTCGCTA 
ATTTCCATAC ATGACAATGA ATTCTTTGGA GATCCCATAG AAGTTGTAGG TCAAAGACAA 
TCATCAATGA TGCCTTTTAC TCTAGAAGAA CTAATAATGT TGTCTCGCTG CCTTCGAGAT 
GCATGCTTAG GGATCATCAA GTTGGCTTAT CCAGAAACAA AACCAGAAGT TCGAGAAGAA 
TATATTACAG CATTTCAAAG TATTGGAGTT ACGACTAATT CTGAAATGCA GCAGTGTATA 
CAGATGGAAC AGAAACGATG GATTCAATTA TTTAAGGTGA TTACCAATCT AGTGAAAATG 
CTCAAGTCCA GAGACACAAG GAGAAATTTT TGTCCTCCAA ATCACTGGCT GTCAGAACAA 
GAAGATATTA AAGCAGATAA GGTCACCCAG CTCTACGTCC CGGCTTCCAG ACACGTGTGG 
AGGTTCCGGC GGATGGGGAG AATTGGCCCC CTGCAGTCGA GCCTGGACGT GGGTTTGGAG 
TCCCCGCCAC TGTCTGTGTC TGAGGAACGA CAGCTCGCCA TCCTGACAGA GCTGCCCTTT 
GTGGTTCCAT TTGAGGAGCG AGTCAAGATC TTTCAAAGGT TGATTTATGC AGATAAGCAA 
GAAGTTCAAG GAGATGGCCC ATTCCTGGAT GGAATTAATG TCACAATACG AAGAAATTAT 
ATTTATGAAG ATGCTTATGA CAAACTTTCC CCAGAAAATG AGCCTGATTT GAAAAAGCGG 
ATCCGTGTGC ACTTACTCAA TGCCCATGGC CTGGATGAAG CTGGCATTGA TGGTGGTGGC 
ATTTTCAGAG AGTTTCTGAA TGAGCTGCTC AAGTCAGGAT TTAACCCCAA CCAGGGGTTC 
TTTAAGACCA CGAATGAAGG GCTTCTGTAC CCTAGCCCAG CCGCTCAGAT GCTCGTGGGA 
GATTCCTTTG CCAGACACTA CTACTTCCTG GGCAGGATGC TTGGAAAGGC TCTCTATGAA 
AATATGCTGG TAGAATTGCC CTTTGCTGGC TTTTTCCTGT CCAAATTGCT TGGAACCAGT 
GCAGATGTGG ACATTCACCA TCTGGCCTCG TTAGATCCTG AAGTGTATAA GAATCTGCTT 
TTTCTCAAGA GCTATGAAGG TGACGTGGAG GAGCTTGGAC TGAACTTCAC CGTGGTGAAC 
AATGACCTGG GGGAAGCACA GGTAGTAGAA CTAAAATTTG GTGGAAAAGA CATCCCTGTC 
ACCAGTGCCA ACAGGATCGC GTACATCCAC CTTGTTGCTG ACTACAGATT GAACAGACAG 
ATCCGCCAGC ACTGCCTGGC CTTCAGACAG GGCCTGGCCA ATGTGGTGAA TCTAGAGTGG 
CTGCGAATGT TTGATCAGCA GGAGATTCAG GTATTAATTT CTGGTGCACA AGTTCCCATA 
AGTCTGGAGG ACCTAAAATC CTTTACAAAT TATTCAGGAG GCTATTCTGC TGACCATCCT 
GTAATCAAAG TCTTCTGGAG AGTTGTGGAA GGGTTCACGG ATGAAGAAAA GCGTAAGCTG 
CTCAAGTTTG TGACCAGCTG CTCCCGACCC CCTCTCCTGG GCTTTAAGGA GCTGTACCCC 
GCATTCTGCA TTCACAACGG GGGCTCTGAC CTTGAGCGGC TCCCCACGGC CAGCACCTGC 
ATGAACCTGC TGAAGCTTCC TGAGTTTTAC GACGAGACCC TTCTGCGGAG TAAGCTCCTC 
TATGCTATCG AATGTGCTGC TGGCTTTGAG CTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_539937.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539937.2 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

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CloneID	OCe18146
Clone ID Related Accession (Same CDS sequence)	XM_539937.6 , XM_539937.5
Accession Version	XM_539937.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin-protein ligase E3C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539937.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCTATGAAAA TATGCTGGTA 
GAATTGCCCT TTGCTGGCTT TTTCCTGTCC AAATTGCTTG GAACCAGTGC AGATGTGGAC 
ATTCACCATC TGGCCTCGTT AGATCCTGAA GTGTATAAGA ATCTGCTTTT TCTCAAGAGC 
TATGAAGGTG ACGTGGAGGA GCTTGGACTG AACTTCACCG TGGTGAACAA TGACCTGGGG 
GAAGCACAGG TAGTAGAACT AAAATTTGGT GGAAAAGACA TCCCTGTCAC CAGTGCCAAC 
AGGATCGCGT ACATCCACCT TGTTGCTGAC TACAGATTGA ACAGACAGAT CCGCCAGCAC 
TGCCTGGCCT TCAGACAGGG CCTGGCCAAT GTGGTGAATC TAGAGTGGCT GCGAATGTTT 
GATCAGCAGG AGATTCAGGT ATTAATTTCT GGTGCACAAG TTCCCATAAG TCTGGAGGAC 
CTAAAATCCT TTACAAATTA TTCAGGAGGC TATTCTGCTG ACCATCCTGT AATCAAAGTC 
TTCTGGAGAG TTGTGGAAGG GTTCACGGAT GAAGAAAAGC GTAAGCTGCT CAAGTTTGTG 
ACCAGCTGCT CCCGACCCCC TCTCCTGGGC TTTAAGGAGC TGTACCCCGC ATTCTGCATT 
CACAACGGGG GCTCTGACCT TGAGCGGCTC CCCACGGCCA GCACCTGCAT GAACCTGCTG 
AAGCTTCCTG AGTTTTACGA CGAGACCCTT CTGCGGAGTA AGCTCCTCTA TGCTATCGAA 
TGTGCTGCTG GCTTTGAGCT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539937.2
CDS	69..3335
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLIWMYQNLIKHSSLFVQQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_539937.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539937.6

ORF Insert Sequence:

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCTATGAAAA TATGCTGGTA 
GAATTGCCCT TTGCTGGCTT TTTCCTGTCC AAATTGCTTG GAACCAGTGC AGATGTGGAC 
ATTCACCATC TGGCCTCGTT AGATCCTGAA GTGTATAAGA ATCTGCTTTT TCTCAAGAGC 
TATGAAGGTG ACGTGGAGGA GCTTGGACTG AACTTCACCG TGGTGAACAA TGACCTGGGG 
GAAGCACAGG TAGTAGAACT AAAATTTGGT GGAAAAGACA TCCCTGTCAC CAGTGCCAAC 
AGGATCGCGT ACATCCACCT TGTTGCTGAC TACAGATTGA ACAGACAGAT CCGCCAGCAC 
TGCCTGGCCT TCAGACAGGG CCTGGCCAAT GTGGTGAATC TAGAGTGGCT GCGAATGTTT 
GATCAGCAGG AGATTCAGGT ATTAATTTCT GGTGCACAAG TTCCCATAAG TCTGGAGGAC 
CTAAAATCCT TTACAAATTA TTCAGGAGGC TATTCTGCTG ACCATCCTGT AATCAAAGTC 
TTCTGGAGAG TTGTGGAAGG GTTCACGGAT GAAGAAAAGC GTAAGCTGCT CAAGTTTGTG 
ACCAGCTGCT CCCGACCCCC TCTCCTGGGC TTTAAGGAGC TGTACCCCGC ATTCTGCATT 
CACAACGGGG GCTCTGACCT TGAGCGGCTC CCCACGGCCA GCACCTGCAT GAACCTGCTG 
AAGCTTCCTG AGTTTTACGA CGAGACCCTT CTGCGGAGTA AGCTCCTCTA TGCTATCGAA 
TGTGCTGCTG GCTTTGAGCT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_539937.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539937.2 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe18146
Clone ID Related Accession (Same CDS sequence)	XM_539937.6 , XM_539937.5
Accession Version	XM_539937.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin-protein ligase E3C isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539937.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCTATGAAAA TATGCTGGTA 
GAATTGCCCT TTGCTGGCTT TTTCCTGTCC AAATTGCTTG GAACCAGTGC AGATGTGGAC 
ATTCACCATC TGGCCTCGTT AGATCCTGAA GTGTATAAGA ATCTGCTTTT TCTCAAGAGC 
TATGAAGGTG ACGTGGAGGA GCTTGGACTG AACTTCACCG TGGTGAACAA TGACCTGGGG 
GAAGCACAGG TAGTAGAACT AAAATTTGGT GGAAAAGACA TCCCTGTCAC CAGTGCCAAC 
AGGATCGCGT ACATCCACCT TGTTGCTGAC TACAGATTGA ACAGACAGAT CCGCCAGCAC 
TGCCTGGCCT TCAGACAGGG CCTGGCCAAT GTGGTGAATC TAGAGTGGCT GCGAATGTTT 
GATCAGCAGG AGATTCAGGT ATTAATTTCT GGTGCACAAG TTCCCATAAG TCTGGAGGAC 
CTAAAATCCT TTACAAATTA TTCAGGAGGC TATTCTGCTG ACCATCCTGT AATCAAAGTC 
TTCTGGAGAG TTGTGGAAGG GTTCACGGAT GAAGAAAAGC GTAAGCTGCT CAAGTTTGTG 
ACCAGCTGCT CCCGACCCCC TCTCCTGGGC TTTAAGGAGC TGTACCCCGC ATTCTGCATT 
CACAACGGGG GCTCTGACCT TGAGCGGCTC CCCACGGCCA GCACCTGCAT GAACCTGCTG 
AAGCTTCCTG AGTTTTACGA CGAGACCCTT CTGCGGAGTA AGCTCCTCTA TGCTATCGAA 
TGTGCTGCTG GCTTTGAGCT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539937.2
CDS	69..3335
Translation	MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLIWMYQNLIKHSSLFVQQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_539937.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539937.5

ORF Insert Sequence:

ATGTTTAGTT TTGAAGGGGA TTTCAAAACG AGGCCCAAAG TGTCTCTCGG CGGCGCCAGC 
AGGAAGGAAG AAAAGGCTTC TCTTTTACAT CGTACTCAGG AAGAAAGAAG GAAGAGAGAG 
GAGGAAAGGC GAAGATTGAA AAATGCAATA ATTATACAGT CATTTATACG AGGCTATAGA 
GACCGGAAAC AGCAACACGC CATCCAGAGA AGCGCCTTTG ATCGCTGCGC CAGTGCTTCA 
CAGTCAGGTG GCACTTTCTC TATTGCCAGT GGGCCCAACC TTACCCTTTT GGTAAGGCAG 
CTTCTGTTTT TCTACAAGCA GAATGAAGAC TCAAAACGTT TGATATGGAT GTATCAGAAC 
TTAATTAAAC ACAGCTCTCT GTTTGTCCAG CAGTTGGATG GATCTGAGAG ACTTACGTGT 
TTATTTCAAA TAAAGAGGTT GATGAGCCTC TGTTGCAGGT TATTGCAAAA CTGTAATGAT 
GACAGTTTGA ATGTTGCACT TCCAATGAGG ATGCTTGAGG TGTTTTCATC TGAGAATACT 
TATTTGCCTG TTTTACATGA CGCTAGCTAT GTGGTGTCCA TAATTGAACA GATTTTGCAC 
TACATGATTC AAAATGGGTA TTATAGATCT CTCTATTTGT TAATTAACAG CAAGCTTCCA 
TCAAGTATTG AGTATTCTGA TTTATCTCGA GTTCCTATAG CAAACATTTT GCTAGAGAAT 
GTTCTGAAAC CATTGCACTT TACTTACAAC TCCTGTCCAG AAGGTGCAAG GCAGCAAGTT 
TTTAGCGCCT TCACTGAGGA GTTTTTGGCA GCACCTTTTA CAGATCAGAT TTTTCATTTC 
ATCATTCCAG CCTTAGCGGA TGCACACACT GTTTTCCCTT ACGAGCCCTT CCTGAGCGCG 
CTGTTGTTAC TGGAGAGCAG ACGCTCCGGG ACGAGCAGCA GGACAAGCAG CAGGGCACCG 
TGGCTCTTCT TCTTTGTCTT AACTGTTGGC GAGCAGTATC TAGGGACCCT CTCTGAGGAA 
GGACTGCTGG TGTATTTAAG AGTACTCCAG ACCTTTCTTT CTCAGCTACC AGTTTCACCG 
GCCAGCACGA GCTGTCAGGA TTCAGCCAGT GACTCGGAAG ATGAGGGTGA AGAGGCAGAC 
AAGCAGCCAA GCGCTCCTGA GGATGGTCGA CTGTCAGTCC CATACATAAC AGAGGAGTGT 
CTGAAGAAGT TGGACACAAA GCAGCAGACC AACACTCTGC TAAACCTGGT ATGGAGGGAC 
TCGGCCAGTG AGGAGGCTTT GACTCTAATG GCGTCGATTG GCCATACACT CATGGTGCAG 
CATCGGATGG TGGTGCCCAA AGTCAGGCTT CTCTACAGTC TAGCCTTTAA TGCCAGGTTT 
CTGAGACATC TTTGGTTTCT CATTTCTTCC ATGACAACAC GGATGATCAC AGGGTCTGTG 
GTGCCACTGC TCCAGGTGAT CTCGAGGGGC TCCCCCATGT CTTTGGAAGA CTCCAGTCGG 
ATCATCCCTC TCTTCTACTT GTTTAGCTCT CTGTTCAGTC ACTCGCTAAT TTCCATACAT 
GACAATGAAT TCTTTGGAGA TCCCATAGAA GTTGTAGGTC AAAGACAATC ATCAATGATG 
CCTTTTACTC TAGAAGAACT AATAATGTTG TCTCGCTGCC TTCGAGATGC ATGCTTAGGG 
ATCATCAAGT TGGCTTATCC AGAAACAAAA CCAGAAGTTC GAGAAGAATA TATTACAGCA 
TTTCAAAGTA TTGGAGTTAC GACTAATTCT GAAATGCAGC AGTGTATACA GATGGAACAG 
AAACGATGGA TTCAATTATT TAAGGTGATT ACCAATCTAG TGAAAATGCT CAAGTCCAGA 
GACACAAGGA GAAATTTTTG TCCTCCAAAT CACTGGCTGT CAGAACAAGA AGATATTAAA 
GCAGATAAGG TCACCCAGCT CTACGTCCCG GCTTCCAGAC ACGTGTGGAG GTTCCGGCGG 
ATGGGGAGAA TTGGCCCCCT GCAGTCGAGC CTGGACGTGG GTTTGGAGTC CCCGCCACTG 
TCTGTGTCTG AGGAACGACA GCTCGCCATC CTGACAGAGC TGCCCTTTGT GGTTCCATTT 
GAGGAGCGAG TCAAGATCTT TCAAAGGTTG ATTTATGCAG ATAAGCAAGA AGTTCAAGGA 
GATGGCCCAT TCCTGGATGG AATTAATGTC ACAATACGAA GAAATTATAT TTATGAAGAT 
GCTTATGACA AACTTTCCCC AGAAAATGAG CCTGATTTGA AAAAGCGGAT CCGTGTGCAC 
TTACTCAATG CCCATGGCCT GGATGAAGCT GGCATTGATG GTGGTGGCAT TTTCAGAGAG 
TTTCTGAATG AGCTGCTCAA GTCAGGATTT AACCCCAACC AGGGGTTCTT TAAGACCACG 
AATGAAGGGC TTCTGTACCC TAGCCCAGCC GCTCAGATGC TCGTGGGAGA TTCCTTTGCC 
AGACACTACT ACTTCCTGGG CAGGATGCTT GGAAAGGCTC TCTATGAAAA TATGCTGGTA 
GAATTGCCCT TTGCTGGCTT TTTCCTGTCC AAATTGCTTG GAACCAGTGC AGATGTGGAC 
ATTCACCATC TGGCCTCGTT AGATCCTGAA GTGTATAAGA ATCTGCTTTT TCTCAAGAGC 
TATGAAGGTG ACGTGGAGGA GCTTGGACTG AACTTCACCG TGGTGAACAA TGACCTGGGG 
GAAGCACAGG TAGTAGAACT AAAATTTGGT GGAAAAGACA TCCCTGTCAC CAGTGCCAAC 
AGGATCGCGT ACATCCACCT TGTTGCTGAC TACAGATTGA ACAGACAGAT CCGCCAGCAC 
TGCCTGGCCT TCAGACAGGG CCTGGCCAAT GTGGTGAATC TAGAGTGGCT GCGAATGTTT 
GATCAGCAGG AGATTCAGGT ATTAATTTCT GGTGCACAAG TTCCCATAAG TCTGGAGGAC 
CTAAAATCCT TTACAAATTA TTCAGGAGGC TATTCTGCTG ACCATCCTGT AATCAAAGTC 
TTCTGGAGAG TTGTGGAAGG GTTCACGGAT GAAGAAAAGC GTAAGCTGCT CAAGTTTGTG 
ACCAGCTGCT CCCGACCCCC TCTCCTGGGC TTTAAGGAGC TGTACCCCGC ATTCTGCATT 
CACAACGGGG GCTCTGACCT TGAGCGGCTC CCCACGGCCA GCACCTGCAT GAACCTGCTG 
AAGCTTCCTG AGTTTTACGA CGAGACCCTT CTGCGGAGTA AGCTCCTCTA TGCTATCGAA 
TGTGCTGCTG GCTTTGAGCT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_014119888.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975363.1 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe18147
Clone ID Related Accession (Same CDS sequence)	XM_014119888.1 , XM_014119888.2
Accession Version	XM_014119888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ubiquitin-protein ligase E3C isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AGGCTTCTCT TTTACATCGT ACTCAGGAAG AAAGAAGGAA GAGAGAGGAG 
GAAAGGCGAA GATTGAAAAA TGCAATAATT ATACAGTCAT TTATACGAGG CTATAGAGAC 
CGGAAACAGC AACACGCCAT CCAGAGAAGC GCCTTTGATC GCTGCGCCAG TGCTTCACAG 
TCAGGTGGCA CTTTCTCTAT TGCCAGTGGG CCCAACCTTA CCCTTTTGGT AAGGCAGCTT 
CTGTTTTTCT ACAAGCAGAA TGAAGACTCA AAACGTTTGA TATGGATGTA TCAGAACTTA 
ATTAAACACA GCTCTCTGTT TGTCCAGCAG TTGGATGGAT CTGAGAGACT TACGTGTTTA 
TTTCAAATAA AGAGGTTGAT GAGCCTCTGT TGCAGGTTAT TGCAAAACTG TAATGATGAC 
AGTTTGAATG TTGCACTTCC AATGAGGATG CTTGAGGTGT TTTCATCTGA GAATACTTAT 
TTGCCTGTTT TACATGACGC TAGCTATGTG GTGTCCATAA TTGAACAGAT TTTGCACTAC 
ATGATTCAAA ATGGGTATTA TAGATCTCTC TATTTGTTAA TTAACAGCAA GCTTCCATCA 
AGTATTGAGT ATTCTGATTT ATCTCGAGTT CCTATAGCAA ACATTTTGCT AGAGAATGTT 
CTGAAACCAT TGCACTTTAC TTACAACTCC TGTCCAGAAG GTGCAAGGCA GCAAGTTTTT 
AGCGCCTTCA CTGAGGAGTT TTTGGCAGCA CCTTTTACAG ATCAGATTTT TCATTTCATC 
ATTCCAGCCT TAGCGGATGC ACACACTGTT TTCCCTTACG AGCCCTTCCT GAGCGCGCTG 
TTGTTACTGG AGAGCAGACG CTCCGGGACG AGCAGCAGGA CAAGCAGCAG GGCACCGTGG 
CTCTTCTTCT TTGTCTTAAC TGTTGGCGAG CAGTATCTAG GGACCCTCTC TGAGGAAGGA 
CTGCTGGTGT ATTTAAGAGT ACTCCAGACC TTTCTTTCTC AGCTACCAGT TTCACCGGCC 
AGCACGAGCT GTCAGGATTC AGCCAGTGAC TCGGAAGATG AGGGTGAAGA GGCAGACAAG 
CAGCCAAGCG CTCCTGAGGA TGGTCGACTG TCAGTCCCAT ACATAACAGA GGAGTGTCTG 
AAGAAGTTGG ACACAAAGCA GCAGACCAAC ACTCTGCTAA ACCTGGTATG GAGGGACTCG 
GCCAGTGAGG AGGCTTTGAC TCTAATGGCG TCGATTGGCC ATACACTCAT GGTGCAGCAT 
CGGATGGTGG TGCCCAAAGT CAGGCTTCTC TACAGTCTAG CCTTTAATGC CAGGTTTCTG 
AGACATCTTT GGTTTCTCAT TTCTTCCATG ACAACACGGA TGATCACAGG GTCTGTGGTG 
CCACTGCTCC AGGTGATCTC GAGGGGCTCC CCCATGTCTT TGGAAGACTC CAGTCGGATC 
ATCCCTCTCT TCTACTTGTT TAGCTCTCTG TTCAGTCACT CGCTAATTTC CATACATGAC 
AATGAATTCT TTGGAGATCC CATAGAAGTT GTAGGTCAAA GACAATCATC AATGATGCCT 
TTTACTCTAG AAGAACTAAT AATGTTGTCT CGCTGCCTTC GAGATGCATG CTTAGGGATC 
ATCAAGTTGG CTTATCCAGA AACAAAACCA GAAGTTCGAG AAGAATATAT TACAGCATTT 
CAAAGTATTG GAGTTACGAC TAATTCTGAA ATGCAGCAGT GTATACAGAT GGAACAGAAA 
CGATGGATTC AATTATTTAA GGTGATTACC AATCTAGTGA AAATGCTCAA GTCCAGAGAC 
ACAAGGAGAA ATTTTTGTCC TCCAAATCAC TGGCTGTCAG AACAAGAAGA TATTAAAGCA 
GATAAGGTCA CCCAGCTCTA CGTCCCGGCT TCCAGACACG TGTGGAGGTT CCGGCGGATG 
GGGAGAATTG GCCCCCTGCA GTCGAGCCTG GACGTGGGTT TGGAGTCCCC GCCACTGTCT 
GTGTCTGAGG AACGACAGCT CGCCATCCTG ACAGAGCTGC CCTTTGTGGT TCCATTTGAG 
GAGCGAGTCA AGATCTTTCA AAGGTTGATT TATGCAGATA AGCAAGAAGT TCAAGGAGAT 
GGCCCATTCC TGGATGGAAT TAATGTCACA ATACGAAGAA ATTATATTTA TGAAGATGCT 
TATGACAAAC TTTCCCCAGA AAATGAGCCT GATTTGAAAA AGCGGATCCG TGTGCACTTA 
CTCAATGCCC ATGGCCTGGA TGAAGCTGGC ATTGATGGTG GTGGCATTTT CAGAGAGTTT 
CTGAATGAGC TGCTCAAGTC AGGATTTAAC CCCAACCAGG GGTTCTTTAA GACCACGAAT 
GAAGGGCTTC TGTACCCTAG CCCAGCCGCT CAGATGCTCG TGGGAGATTC CTTTGCCAGA 
CACTACTACT TCCTGGGCAG GATGCTTGGA AAGGCTCTCT ATGAAAATAT GCTGGTAGAA 
TTGCCCTTTG CTGGCTTTTT CCTGTCCAAA TTGCTTGGAA CCAGTGCAGA TGTGGACATT 
CACCATCTGG CCTCGTTAGA TCCTGAAGTG TATAAGAATC TGCTTTTTCT CAAGAGCTAT 
GAAGGTGACG TGGAGGAGCT TGGACTGAAC TTCACCGTGG TGAACAATGA CCTGGGGGAA 
GCACAGGTAG TAGAACTAAA ATTTGGTGGA AAAGACATCC CTGTCACCAG TGCCAACAGG 
ATCGCGTACA TCCACCTTGT TGCTGACTAC AGATTGAACA GACAGATCCG CCAGCACTGC 
CTGGCCTTCA GACAGGGCCT GGCCAATGTG GTGAATCTAG AGTGGCTGCG AATGTTTGAT 
CAGCAGGAGA TTCAGGTATT AATTTCTGGT GCACAAGTTC CCATAAGTCT GGAGGACCTA 
AAATCCTTTA CAAATTATTC AGGAGGCTAT TCTGCTGACC ATCCTGTAAT CAAAGTCTTC 
TGGAGAGTTG TGGAAGGGTT CACGGATGAA GAAAAGCGTA AGCTGCTCAA GTTTGTGACC 
AGCTGCTCCC GACCCCCTCT CCTGGGCTTT AAGGAGCTGT ACCCCGCATT CTGCATTCAC 
AACGGGGGCT CTGACCTTGA GCGGCTCCCC ACGGCCAGCA CCTGCATGAA CCTGCTGAAG 
CTTCCTGAGT TTTACGACGA GACCCTTCTG CGGAGTAAGC TCCTCTATGC TATCGAATGT 
GCTGCTGGCT TTGAGCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975363.1
CDS	433..3636
Translation	MEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLIWMYQNLIKHSSLFVQQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_014119888.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119888.1

ORF Insert Sequence:

ATGGAAGAAA AGGCTTCTCT TTTACATCGT ACTCAGGAAG AAAGAAGGAA GAGAGAGGAG 
GAAAGGCGAA GATTGAAAAA TGCAATAATT ATACAGTCAT TTATACGAGG CTATAGAGAC 
CGGAAACAGC AACACGCCAT CCAGAGAAGC GCCTTTGATC GCTGCGCCAG TGCTTCACAG 
TCAGGTGGCA CTTTCTCTAT TGCCAGTGGG CCCAACCTTA CCCTTTTGGT AAGGCAGCTT 
CTGTTTTTCT ACAAGCAGAA TGAAGACTCA AAACGTTTGA TATGGATGTA TCAGAACTTA 
ATTAAACACA GCTCTCTGTT TGTCCAGCAG TTGGATGGAT CTGAGAGACT TACGTGTTTA 
TTTCAAATAA AGAGGTTGAT GAGCCTCTGT TGCAGGTTAT TGCAAAACTG TAATGATGAC 
AGTTTGAATG TTGCACTTCC AATGAGGATG CTTGAGGTGT TTTCATCTGA GAATACTTAT 
TTGCCTGTTT TACATGACGC TAGCTATGTG GTGTCCATAA TTGAACAGAT TTTGCACTAC 
ATGATTCAAA ATGGGTATTA TAGATCTCTC TATTTGTTAA TTAACAGCAA GCTTCCATCA 
AGTATTGAGT ATTCTGATTT ATCTCGAGTT CCTATAGCAA ACATTTTGCT AGAGAATGTT 
CTGAAACCAT TGCACTTTAC TTACAACTCC TGTCCAGAAG GTGCAAGGCA GCAAGTTTTT 
AGCGCCTTCA CTGAGGAGTT TTTGGCAGCA CCTTTTACAG ATCAGATTTT TCATTTCATC 
ATTCCAGCCT TAGCGGATGC ACACACTGTT TTCCCTTACG AGCCCTTCCT GAGCGCGCTG 
TTGTTACTGG AGAGCAGACG CTCCGGGACG AGCAGCAGGA CAAGCAGCAG GGCACCGTGG 
CTCTTCTTCT TTGTCTTAAC TGTTGGCGAG CAGTATCTAG GGACCCTCTC TGAGGAAGGA 
CTGCTGGTGT ATTTAAGAGT ACTCCAGACC TTTCTTTCTC AGCTACCAGT TTCACCGGCC 
AGCACGAGCT GTCAGGATTC AGCCAGTGAC TCGGAAGATG AGGGTGAAGA GGCAGACAAG 
CAGCCAAGCG CTCCTGAGGA TGGTCGACTG TCAGTCCCAT ACATAACAGA GGAGTGTCTG 
AAGAAGTTGG ACACAAAGCA GCAGACCAAC ACTCTGCTAA ACCTGGTATG GAGGGACTCG 
GCCAGTGAGG AGGCTTTGAC TCTAATGGCG TCGATTGGCC ATACACTCAT GGTGCAGCAT 
CGGATGGTGG TGCCCAAAGT CAGGCTTCTC TACAGTCTAG CCTTTAATGC CAGGTTTCTG 
AGACATCTTT GGTTTCTCAT TTCTTCCATG ACAACACGGA TGATCACAGG GTCTGTGGTG 
CCACTGCTCC AGGTGATCTC GAGGGGCTCC CCCATGTCTT TGGAAGACTC CAGTCGGATC 
ATCCCTCTCT TCTACTTGTT TAGCTCTCTG TTCAGTCACT CGCTAATTTC CATACATGAC 
AATGAATTCT TTGGAGATCC CATAGAAGTT GTAGGTCAAA GACAATCATC AATGATGCCT 
TTTACTCTAG AAGAACTAAT AATGTTGTCT CGCTGCCTTC GAGATGCATG CTTAGGGATC 
ATCAAGTTGG CTTATCCAGA AACAAAACCA GAAGTTCGAG AAGAATATAT TACAGCATTT 
CAAAGTATTG GAGTTACGAC TAATTCTGAA ATGCAGCAGT GTATACAGAT GGAACAGAAA 
CGATGGATTC AATTATTTAA GGTGATTACC AATCTAGTGA AAATGCTCAA GTCCAGAGAC 
ACAAGGAGAA ATTTTTGTCC TCCAAATCAC TGGCTGTCAG AACAAGAAGA TATTAAAGCA 
GATAAGGTCA CCCAGCTCTA CGTCCCGGCT TCCAGACACG TGTGGAGGTT CCGGCGGATG 
GGGAGAATTG GCCCCCTGCA GTCGAGCCTG GACGTGGGTT TGGAGTCCCC GCCACTGTCT 
GTGTCTGAGG AACGACAGCT CGCCATCCTG ACAGAGCTGC CCTTTGTGGT TCCATTTGAG 
GAGCGAGTCA AGATCTTTCA AAGGTTGATT TATGCAGATA AGCAAGAAGT TCAAGGAGAT 
GGCCCATTCC TGGATGGAAT TAATGTCACA ATACGAAGAA ATTATATTTA TGAAGATGCT 
TATGACAAAC TTTCCCCAGA AAATGAGCCT GATTTGAAAA AGCGGATCCG TGTGCACTTA 
CTCAATGCCC ATGGCCTGGA TGAAGCTGGC ATTGATGGTG GTGGCATTTT CAGAGAGTTT 
CTGAATGAGC TGCTCAAGTC AGGATTTAAC CCCAACCAGG GGTTCTTTAA GACCACGAAT 
GAAGGGCTTC TGTACCCTAG CCCAGCCGCT CAGATGCTCG TGGGAGATTC CTTTGCCAGA 
CACTACTACT TCCTGGGCAG GATGCTTGGA AAGGCTCTCT ATGAAAATAT GCTGGTAGAA 
TTGCCCTTTG CTGGCTTTTT CCTGTCCAAA TTGCTTGGAA CCAGTGCAGA TGTGGACATT 
CACCATCTGG CCTCGTTAGA TCCTGAAGTG TATAAGAATC TGCTTTTTCT CAAGAGCTAT 
GAAGGTGACG TGGAGGAGCT TGGACTGAAC TTCACCGTGG TGAACAATGA CCTGGGGGAA 
GCACAGGTAG TAGAACTAAA ATTTGGTGGA AAAGACATCC CTGTCACCAG TGCCAACAGG 
ATCGCGTACA TCCACCTTGT TGCTGACTAC AGATTGAACA GACAGATCCG CCAGCACTGC 
CTGGCCTTCA GACAGGGCCT GGCCAATGTG GTGAATCTAG AGTGGCTGCG AATGTTTGAT 
CAGCAGGAGA TTCAGGTATT AATTTCTGGT GCACAAGTTC CCATAAGTCT GGAGGACCTA 
AAATCCTTTA CAAATTATTC AGGAGGCTAT TCTGCTGACC ATCCTGTAAT CAAAGTCTTC 
TGGAGAGTTG TGGAAGGGTT CACGGATGAA GAAAAGCGTA AGCTGCTCAA GTTTGTGACC 
AGCTGCTCCC GACCCCCTCT CCTGGGCTTT AAGGAGCTGT ACCCCGCATT CTGCATTCAC 
AACGGGGGCT CTGACCTTGA GCGGCTCCCC ACGGCCAGCA CCTGCATGAA CCTGCTGAAG 
CTTCCTGAGT TTTACGACGA GACCCTTCTG CGGAGTAAGC TCCTCTATGC TATCGAATGT 
GCTGCTGGCT TTGAGCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE3C ( XM_014119888.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975363.1 Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.

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CloneID	OCe18147
Clone ID Related Accession (Same CDS sequence)	XM_014119888.1 , XM_014119888.2
Accession Version	XM_014119888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ubiquitin-protein ligase E3C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAA AGGCTTCTCT TTTACATCGT ACTCAGGAAG AAAGAAGGAA GAGAGAGGAG 
GAAAGGCGAA GATTGAAAAA TGCAATAATT ATACAGTCAT TTATACGAGG CTATAGAGAC 
CGGAAACAGC AACACGCCAT CCAGAGAAGC GCCTTTGATC GCTGCGCCAG TGCTTCACAG 
TCAGGTGGCA CTTTCTCTAT TGCCAGTGGG CCCAACCTTA CCCTTTTGGT AAGGCAGCTT 
CTGTTTTTCT ACAAGCAGAA TGAAGACTCA AAACGTTTGA TATGGATGTA TCAGAACTTA 
ATTAAACACA GCTCTCTGTT TGTCCAGCAG TTGGATGGAT CTGAGAGACT TACGTGTTTA 
TTTCAAATAA AGAGGTTGAT GAGCCTCTGT TGCAGGTTAT TGCAAAACTG TAATGATGAC 
AGTTTGAATG TTGCACTTCC AATGAGGATG CTTGAGGTGT TTTCATCTGA GAATACTTAT 
TTGCCTGTTT TACATGACGC TAGCTATGTG GTGTCCATAA TTGAACAGAT TTTGCACTAC 
ATGATTCAAA ATGGGTATTA TAGATCTCTC TATTTGTTAA TTAACAGCAA GCTTCCATCA 
AGTATTGAGT ATTCTGATTT ATCTCGAGTT CCTATAGCAA ACATTTTGCT AGAGAATGTT 
CTGAAACCAT TGCACTTTAC TTACAACTCC TGTCCAGAAG GTGCAAGGCA GCAAGTTTTT 
AGCGCCTTCA CTGAGGAGTT TTTGGCAGCA CCTTTTACAG ATCAGATTTT TCATTTCATC 
ATTCCAGCCT TAGCGGATGC ACACACTGTT TTCCCTTACG AGCCCTTCCT GAGCGCGCTG 
TTGTTACTGG AGAGCAGACG CTCCGGGACG AGCAGCAGGA CAAGCAGCAG GGCACCGTGG 
CTCTTCTTCT TTGTCTTAAC TGTTGGCGAG CAGTATCTAG GGACCCTCTC TGAGGAAGGA 
CTGCTGGTGT ATTTAAGAGT ACTCCAGACC TTTCTTTCTC AGCTACCAGT TTCACCGGCC 
AGCACGAGCT GTCAGGATTC AGCCAGTGAC TCGGAAGATG AGGGTGAAGA GGCAGACAAG 
CAGCCAAGCG CTCCTGAGGA TGGTCGACTG TCAGTCCCAT ACATAACAGA GGAGTGTCTG 
AAGAAGTTGG ACACAAAGCA GCAGACCAAC ACTCTGCTAA ACCTGGTATG GAGGGACTCG 
GCCAGTGAGG AGGCTTTGAC TCTAATGGCG TCGATTGGCC ATACACTCAT GGTGCAGCAT 
CGGATGGTGG TGCCCAAAGT CAGGCTTCTC TACAGTCTAG CCTTTAATGC CAGGTTTCTG 
AGACATCTTT GGTTTCTCAT TTCTTCCATG ACAACACGGA TGATCACAGG GTCTGTGGTG 
CCACTGCTCC AGGTGATCTC GAGGGGCTCC CCCATGTCTT TGGAAGACTC CAGTCGGATC 
ATCCCTCTCT TCTACTTGTT TAGCTCTCTG TTCAGTCACT CGCTAATTTC CATACATGAC 
AATGAATTCT TTGGAGATCC CATAGAAGTT GTAGGTCAAA GACAATCATC AATGATGCCT 
TTTACTCTAG AAGAACTAAT AATGTTGTCT CGCTGCCTTC GAGATGCATG CTTAGGGATC 
ATCAAGTTGG CTTATCCAGA AACAAAACCA GAAGTTCGAG AAGAATATAT TACAGCATTT 
CAAAGTATTG GAGTTACGAC TAATTCTGAA ATGCAGCAGT GTATACAGAT GGAACAGAAA 
CGATGGATTC AATTATTTAA GGTGATTACC AATCTAGTGA AAATGCTCAA GTCCAGAGAC 
ACAAGGAGAA ATTTTTGTCC TCCAAATCAC TGGCTGTCAG AACAAGAAGA TATTAAAGCA 
GATAAGGTCA CCCAGCTCTA CGTCCCGGCT TCCAGACACG TGTGGAGGTT CCGGCGGATG 
GGGAGAATTG GCCCCCTGCA GTCGAGCCTG GACGTGGGTT TGGAGTCCCC GCCACTGTCT 
GTGTCTGAGG AACGACAGCT CGCCATCCTG ACAGAGCTGC CCTTTGTGGT TCCATTTGAG 
GAGCGAGTCA AGATCTTTCA AAGGTTGATT TATGCAGATA AGCAAGAAGT TCAAGGAGAT 
GGCCCATTCC TGGATGGAAT TAATGTCACA ATACGAAGAA ATTATATTTA TGAAGATGCT 
TATGACAAAC TTTCCCCAGA AAATGAGCCT GATTTGAAAA AGCGGATCCG TGTGCACTTA 
CTCAATGCCC ATGGCCTGGA TGAAGCTGGC ATTGATGGTG GTGGCATTTT CAGAGAGTTT 
CTGAATGAGC TGCTCAAGTC AGGATTTAAC CCCAACCAGG GGTTCTTTAA GACCACGAAT 
GAAGGGCTTC TGTACCCTAG CCCAGCCGCT CAGATGCTCG TGGGAGATTC CTTTGCCAGA 
CACTACTACT TCCTGGGCAG GATGCTTGGA AAGGCTCTCT ATGAAAATAT GCTGGTAGAA 
TTGCCCTTTG CTGGCTTTTT CCTGTCCAAA TTGCTTGGAA CCAGTGCAGA TGTGGACATT 
CACCATCTGG CCTCGTTAGA TCCTGAAGTG TATAAGAATC TGCTTTTTCT CAAGAGCTAT 
GAAGGTGACG TGGAGGAGCT TGGACTGAAC TTCACCGTGG TGAACAATGA CCTGGGGGAA 
GCACAGGTAG TAGAACTAAA ATTTGGTGGA AAAGACATCC CTGTCACCAG TGCCAACAGG 
ATCGCGTACA TCCACCTTGT TGCTGACTAC AGATTGAACA GACAGATCCG CCAGCACTGC 
CTGGCCTTCA GACAGGGCCT GGCCAATGTG GTGAATCTAG AGTGGCTGCG AATGTTTGAT 
CAGCAGGAGA TTCAGGTATT AATTTCTGGT GCACAAGTTC CCATAAGTCT GGAGGACCTA 
AAATCCTTTA CAAATTATTC AGGAGGCTAT TCTGCTGACC ATCCTGTAAT CAAAGTCTTC 
TGGAGAGTTG TGGAAGGGTT CACGGATGAA GAAAAGCGTA AGCTGCTCAA GTTTGTGACC 
AGCTGCTCCC GACCCCCTCT CCTGGGCTTT AAGGAGCTGT ACCCCGCATT CTGCATTCAC 
AACGGGGGCT CTGACCTTGA GCGGCTCCCC ACGGCCAGCA CCTGCATGAA CCTGCTGAAG 
CTTCCTGAGT TTTACGACGA GACCCTTCTG CGGAGTAAGC TCCTCTATGC TATCGAATGT 
GCTGCTGGCT TTGAGCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975363.1
CDS	489..3692
Translation	MEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQHAIQRSAFDRCASASQSGGTFSIASGPNLTLLVRQLLFFYKQNEDSKRLIWMYQNLIKHSSLFVQQLDGSERLTCLFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLHDASYVVSIIEQILHYMIQNGYYRSLYLLINSKLPSSIEYSDLSRVPIANILLENVLKPLHFTYNSCPEGARQQVFSAFTEEFLAAPFTDQIFHFIIPALADAHTVFPYEPFLSALLLLESRRSGTSSRTSSRAPWLFFFVLTVGEQYLGTLSEEGLLVYLRVLQTFLSQLPVSPASTSCQDSASDSEDEGEEADKQPSAPEDGRLSVPYITEECLKKLDTKQQTNTLLNLVWRDSASEEALTLMASIGHTLMVQHRMVVPKVRLLYSLAFNARFLRHLWFLISSMTTRMITGSVVPLLQVISRGSPMSLEDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLAYPETKPEVREEYITAFQSIGVTTNSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSEQEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSSLDVGLESPPLSVSEERQLAILTELPFVVPFEERVKIFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGGGIFREFLNELLKSGFNPNQGFFKTTNEGLLYPSPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFAGFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEGDVEELGLNFTVVNNDLGEAQVVELKFGGKDIPVTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVNLEWLRMFDQQEIQVLISGAQVPISLEDLKSFTNYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTASTCMNLLKLPEFYDETLLRSKLLYAIECAAGFELS

Target ORF information:

RefSeq Version	XM_014119888.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin protein ligase E3C (UBE3C), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119888.2

ORF Insert Sequence:

ATGGAAGAAA AGGCTTCTCT TTTACATCGT ACTCAGGAAG AAAGAAGGAA GAGAGAGGAG 
GAAAGGCGAA GATTGAAAAA TGCAATAATT ATACAGTCAT TTATACGAGG CTATAGAGAC 
CGGAAACAGC AACACGCCAT CCAGAGAAGC GCCTTTGATC GCTGCGCCAG TGCTTCACAG 
TCAGGTGGCA CTTTCTCTAT TGCCAGTGGG CCCAACCTTA CCCTTTTGGT AAGGCAGCTT 
CTGTTTTTCT ACAAGCAGAA TGAAGACTCA AAACGTTTGA TATGGATGTA TCAGAACTTA 
ATTAAACACA GCTCTCTGTT TGTCCAGCAG TTGGATGGAT CTGAGAGACT TACGTGTTTA 
TTTCAAATAA AGAGGTTGAT GAGCCTCTGT TGCAGGTTAT TGCAAAACTG TAATGATGAC 
AGTTTGAATG TTGCACTTCC AATGAGGATG CTTGAGGTGT TTTCATCTGA GAATACTTAT 
TTGCCTGTTT TACATGACGC TAGCTATGTG GTGTCCATAA TTGAACAGAT TTTGCACTAC 
ATGATTCAAA ATGGGTATTA TAGATCTCTC TATTTGTTAA TTAACAGCAA GCTTCCATCA 
AGTATTGAGT ATTCTGATTT ATCTCGAGTT CCTATAGCAA ACATTTTGCT AGAGAATGTT 
CTGAAACCAT TGCACTTTAC TTACAACTCC TGTCCAGAAG GTGCAAGGCA GCAAGTTTTT 
AGCGCCTTCA CTGAGGAGTT TTTGGCAGCA CCTTTTACAG ATCAGATTTT TCATTTCATC 
ATTCCAGCCT TAGCGGATGC ACACACTGTT TTCCCTTACG AGCCCTTCCT GAGCGCGCTG 
TTGTTACTGG AGAGCAGACG CTCCGGGACG AGCAGCAGGA CAAGCAGCAG GGCACCGTGG 
CTCTTCTTCT TTGTCTTAAC TGTTGGCGAG CAGTATCTAG GGACCCTCTC TGAGGAAGGA 
CTGCTGGTGT ATTTAAGAGT ACTCCAGACC TTTCTTTCTC AGCTACCAGT TTCACCGGCC 
AGCACGAGCT GTCAGGATTC AGCCAGTGAC TCGGAAGATG AGGGTGAAGA GGCAGACAAG 
CAGCCAAGCG CTCCTGAGGA TGGTCGACTG TCAGTCCCAT ACATAACAGA GGAGTGTCTG 
AAGAAGTTGG ACACAAAGCA GCAGACCAAC ACTCTGCTAA ACCTGGTATG GAGGGACTCG 
GCCAGTGAGG AGGCTTTGAC TCTAATGGCG TCGATTGGCC ATACACTCAT GGTGCAGCAT 
CGGATGGTGG TGCCCAAAGT CAGGCTTCTC TACAGTCTAG CCTTTAATGC CAGGTTTCTG 
AGACATCTTT GGTTTCTCAT TTCTTCCATG ACAACACGGA TGATCACAGG GTCTGTGGTG 
CCACTGCTCC AGGTGATCTC GAGGGGCTCC CCCATGTCTT TGGAAGACTC CAGTCGGATC 
ATCCCTCTCT TCTACTTGTT TAGCTCTCTG TTCAGTCACT CGCTAATTTC CATACATGAC 
AATGAATTCT TTGGAGATCC CATAGAAGTT GTAGGTCAAA GACAATCATC AATGATGCCT 
TTTACTCTAG AAGAACTAAT AATGTTGTCT CGCTGCCTTC GAGATGCATG CTTAGGGATC 
ATCAAGTTGG CTTATCCAGA AACAAAACCA GAAGTTCGAG AAGAATATAT TACAGCATTT 
CAAAGTATTG GAGTTACGAC TAATTCTGAA ATGCAGCAGT GTATACAGAT GGAACAGAAA 
CGATGGATTC AATTATTTAA GGTGATTACC AATCTAGTGA AAATGCTCAA GTCCAGAGAC 
ACAAGGAGAA ATTTTTGTCC TCCAAATCAC TGGCTGTCAG AACAAGAAGA TATTAAAGCA 
GATAAGGTCA CCCAGCTCTA CGTCCCGGCT TCCAGACACG TGTGGAGGTT CCGGCGGATG 
GGGAGAATTG GCCCCCTGCA GTCGAGCCTG GACGTGGGTT TGGAGTCCCC GCCACTGTCT 
GTGTCTGAGG AACGACAGCT CGCCATCCTG ACAGAGCTGC CCTTTGTGGT TCCATTTGAG 
GAGCGAGTCA AGATCTTTCA AAGGTTGATT TATGCAGATA AGCAAGAAGT TCAAGGAGAT 
GGCCCATTCC TGGATGGAAT TAATGTCACA ATACGAAGAA ATTATATTTA TGAAGATGCT 
TATGACAAAC TTTCCCCAGA AAATGAGCCT GATTTGAAAA AGCGGATCCG TGTGCACTTA 
CTCAATGCCC ATGGCCTGGA TGAAGCTGGC ATTGATGGTG GTGGCATTTT CAGAGAGTTT 
CTGAATGAGC TGCTCAAGTC AGGATTTAAC CCCAACCAGG GGTTCTTTAA GACCACGAAT 
GAAGGGCTTC TGTACCCTAG CCCAGCCGCT CAGATGCTCG TGGGAGATTC CTTTGCCAGA 
CACTACTACT TCCTGGGCAG GATGCTTGGA AAGGCTCTCT ATGAAAATAT GCTGGTAGAA 
TTGCCCTTTG CTGGCTTTTT CCTGTCCAAA TTGCTTGGAA CCAGTGCAGA TGTGGACATT 
CACCATCTGG CCTCGTTAGA TCCTGAAGTG TATAAGAATC TGCTTTTTCT CAAGAGCTAT 
GAAGGTGACG TGGAGGAGCT TGGACTGAAC TTCACCGTGG TGAACAATGA CCTGGGGGAA 
GCACAGGTAG TAGAACTAAA ATTTGGTGGA AAAGACATCC CTGTCACCAG TGCCAACAGG 
ATCGCGTACA TCCACCTTGT TGCTGACTAC AGATTGAACA GACAGATCCG CCAGCACTGC 
CTGGCCTTCA GACAGGGCCT GGCCAATGTG GTGAATCTAG AGTGGCTGCG AATGTTTGAT 
CAGCAGGAGA TTCAGGTATT AATTTCTGGT GCACAAGTTC CCATAAGTCT GGAGGACCTA 
AAATCCTTTA CAAATTATTC AGGAGGCTAT TCTGCTGACC ATCCTGTAAT CAAAGTCTTC 
TGGAGAGTTG TGGAAGGGTT CACGGATGAA GAAAAGCGTA AGCTGCTCAA GTTTGTGACC 
AGCTGCTCCC GACCCCCTCT CCTGGGCTTT AAGGAGCTGT ACCCCGCATT CTGCATTCAC 
AACGGGGGCT CTGACCTTGA GCGGCTCCCC ACGGCCAGCA CCTGCATGAA CCTGCTGAAG 
CTTCCTGAGT TTTACGACGA GACCCTTCTG CGGAGTAAGC TCCTCTATGC TATCGAATGT 
GCTGCTGGCT TTGAGCTGAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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UBE3C cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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