PLEKHA8 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLEKHA8
Entrez Gene ID	482381
Full Name	pleckstrin homology domain containing A8
Synonyms	FAPP-2,FAPP2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022427143.1	XP_022282851.1	pleckstrin homology domain-containing family A member 8 isoform X2
XM_022427144.1	XP_022282852.1	pleckstrin homology domain-containing family A member 8 isoform X3
XM_022427145.1	XP_022282853.1	pleckstrin homology domain-containing family A member 8 isoform X3
NM_001167698.1	NP_001161170.1	pleckstrin homology domain-containing family A member 8
XM_022427142.1	XP_022282850.1	pleckstrin homology domain-containing family A member 8 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLEKHA8	NP_001161170.1
Rattus norvegicus (Norway rat)	Plekha8	NP_001102705.1
Xenopus tropicalis (tropical clawed frog)	plekha8	XP_002932833.2
Bos taurus (cattle)	PLEKHA8	NP_001183961.1
Danio rerio (zebrafish)	plekha8	NP_001007375.1
Kluyveromyces lactis	KLLA0C14476g	XP_452848.1
Arabidopsis thaliana (thale cress)	GLTP1	NP_973588.1
Homo sapiens (human)	PLEKHA8	NP_001183955.1
Pan troglodytes (chimpanzee)	PLEKHA8	NP_001186008.1
	PLEKHA8	XP_001086395.1
Gallus gallus (chicken)	PLEKHA8	XP_418736.3
Eremothecium gossypii	AGOS_ABL064W	NP_982883.1
Magnaporthe oryzae (rice blast fungus)	MGG_09396	XP_003720215.1
Oryza sativa (rice)	Os02g0622400	NP_001047468.1
Mus musculus (house mouse)	Plekha8	NP_001157833.1
Neurospora crassa	NCU07947	XP_962080.1

Gene Ontology
Bibliography

Related articles in PubMed

FAPP2 is required for aquaporin-2 apical sorting at trans-Golgi network in polarized MDCK cells.
Yui N, Okutsu R, Sohara E, Rai T, Ohta A, Noda Y, Sasaki S, Uchida S
American journal of physiology. Cell physiology297(6)C1389-96(2009 Dec)

Golgi protein FAPP2 tubulates membranes.
Cao X, Coskun U, R?ssle M, Buschhorn SB, Grzybek M, Dafforn TR, Lenoir M, Overduin M, Simons K
Proceedings of the National Academy of Sciences of the United States of America106(50)21121-5(2009 Dec)

Genome sequence, comparative analysis and haplotype structure of the domestic dog.
Lindblad-Toh K, Wade CM, Mikkelsen TS, Karlsson EK, Jaffe DB, Kamal M, Clamp M, Chang JL, Kulbokas EJ 3rd, Zody MC, Mauceli E, Xie X, Breen M, Wayne RK, Ostrander EA, Ponting CP, Galibert F, Smith DR, DeJong PJ, Kirkness E, Alvarez P, Biagi T, Brockman W, Butler J, Chin CW, Cook A, Cuff J, Daly MJ, DeCaprio D, Gnerre S, Grabherr M, Kellis M, Kleber M, Bardeleben C, Goodstadt L, Heger A, Hitte C, Kim L, Koepfli KP, Parker HG, Pollinger JP, Searle SM, Sutter NB, Thomas R, Webber C, Baldwin J, Abebe A, Abouelleil A, Aftuck L, Ait-Zahra M, Aldredge T, Allen N, An P, Anderson S, Antoine C, Arachchi H, Aslam A, Ayotte L, Bachantsang P, Barry A, Bayul T, Benamara M, Berlin A, Bessette D, Blitshteyn B, Bloom T, Blye J, Boguslavskiy L, Bonnet C, Boukhgalter B, Brown A, Cahill P, Calixte N, Camarata J, Cheshatsang Y, Chu J, Citroen M, Collymore A, Cooke P, Dawoe T, Daza R, Decktor K, DeGray S, Dhargay N, Dooley K, Dooley K, Dorje P, Dorjee K, Dorris L, Duffey N, Dupes A, Egbiremolen O, Elong R, Falk J, Farina A, Faro S, Ferguson D, Ferreira P, Fisher S, FitzGerald M, Foley K, Foley C, Franke A, Friedrich D, Gage D, Garber M, Gearin G, Giannoukos G, Goode T, Goyette A, Graham J, Grandbois E, Gyaltsen K, Hafez N, Hagopian D, Hagos B, Hall J, Healy C, Hegarty R, Honan T, Horn A, Houde N, Hughes L, Hunnicutt L, Husby M, Jester B, Jones C, Kamat A, Kanga B, Kells C, Khazanovich D, Kieu AC, Kisner P, Kumar M, Lance K, Landers T, Lara M, Lee W, Leger JP, Lennon N, Leuper L, LeVine S, Liu J, Liu X, Lokyitsang Y, Lokyitsang T, Lui A, Macdonald J, Major J, Marabella R, Maru K, Matthews C, McDonough S, Mehta T, Meldrim J, Melnikov A, Meneus L, Mihalev A, Mihova T, Miller K, Mittelman R, Mlenga V, Mulrain L, Munson G, Navidi A, Naylor J, Nguyen T, Nguyen N, Nguyen C, Nguyen T, Nicol R, Norbu N, Norbu C, Novod N, Nyima T, Olandt P, O'Neill B, O'Neill K, Osman S, Oyono L, Patti C, Perrin D, Phunkhang P, Pierre F, Priest M, Rachupka A, Raghuraman S, Rameau R, Ray V, Raymond C, Rege F, Rise C, Rogers J, Rogov P, Sahalie J, Settipalli S, Sharpe T, Shea T, Sheehan M, Sherpa N, Shi J, Shih D, Sloan J, Smith C, Sparrow T, Stalker J, Stange-Thomann N, Stavropoulos S, Stone C, Stone S, Sykes S, Tchuinga P, Tenzing P, Tesfaye S, Thoulutsang D, Thoulutsang Y, Topham K, Topping I, Tsamla T, Vassiliev H, Venkataraman V, Vo A, Wangchuk T, Wangdi T, Weiand M, Wilkinson J, Wilson A, Yadav S, Yang S, Yang X, Young G, Yu Q, Zainoun J, Zembek L, Zimmer A, Lander ES
Nature438(7069)803-19(2005 Dec)

FAPP2 is involved in the transport of apical cargo in polarized MDCK cells.
Vieira OV, Verkade P, Manninen A, Simons K
The Journal of cell biology170(4)521-6(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

purified FAPP2 protein has the capability to form tubules from membrane sheets in vitro. This activity is dependent on the phosphoinositide-binding activity of the PH domain of FAPP2
Title: Golgi protein FAPP2 tubulates membranes.

FAPP2 plays a specific role in apical transport in MDCK cells.
Title: FAPP2 is involved in the transport of apical cargo in polarized MDCK cells.

The following PLEKHA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLEKHA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe00329	NM_001167698.1 Latest version!	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe293085	XM_022427145.1 Latest version!	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe293085	XM_022427144.1 Latest version!	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OCe293083	XM_022427142.1 Latest version!	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe293084	XM_022427143.1 Latest version!	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLEKHA8 ( NM_001167698.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001161170.1 Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), mRNA.

Price & Availability↑

CloneID	OCe00329
Clone ID Related Accession (Same CDS sequence)	NM_001167698.1
Accession Version	NM_001167698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology domain-containing family A member 8
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03009401.1 and AAEX03009402.1. On Nov 12, 2009 this sequence version replaced XM_539498.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GU204956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGGG TGCTGTACAA GTGGACCAAC TATCTGAGCG GTTGGCAGCC TAGATGGTTC 
CTACTCTGTG GGGGTATATT ATCCTATTAT GATTCTCCTG AAGATGCCTG GAAAGGTTGC 
AAAGGGAGTA TCCAAATGGC GGTCTGTGAA ATTCAAGTTC ATTCTGTAGA TAACACACGC 
ATGGACCTGA TAATCCCTGG GGAGCAGTAT TTCTACTTGA AGGCCAGAAG TGTGGCTGAG 
AGACAGCGGT GGCTGGTAGC CCTGGGGTCA GCCAAGGCTT GCCTGACAGA TAGTAGGACC 
CAGAAGGAAA AAGAGTTTGC TGAAAACACT GAAAACTTGA AAACCAAAAT GTCGGAACTA 
AGACTCTACT GTGACCTCCT TGTTCAGCAA GTAGATAAAA CAAAAGAAGT AACCACAACT 
GGTGTGTCCA ATTCTGAGGA AGGAATTGAT GTGGGAACTC TGCTGAAATC AACCTGCAAT 
ACTTTTCTGA AGACTTTGGA AGAATGCATG CAGATCGCAA ACGCAGCCTT CACCTCTGAA 
CTACTCTATC GTACTCCCCC CGGCTCACCA CAGCTGGCCA TGCTCAAGTC CAGCAAGATG 
AAACATCCTA TTATACCGAT TCATAATTCA TTGGAAAGGC AAATGGAGTT AAACAGTTGT 
GAAAATGGAT CTTTGCATAT GGAAATAAAT GATGGTGAAG AAATCCTAAT GAAAAATAAG 
AGCTCCTTAT ATTTGAAACC AGAGATAGAT TGCAGCATAT CAAGTGAAGA AAATACAGAT 
GATAATATAA CAGTCCAAGG TGAGATCATG AAGGAAGAAG GAGAGGACAA CCTGGGAAAT 
CATGACAGCA GCTTAGCACA GCCTGCATCA GACTCCTCAA GTAGCCCTCC AGAATCCCAC 
TGGGAAGAAG GCCAAGAAAT CATCCCAACT TTCTTTAGTA CCATGAATAC CAGCTTTAGT 
GACATTGAAC TTCTGGAAGA CAGTGGCATT CCCACAGAAG CATTCTTGGC ATCATGTTAT 
GCTGTGGTTC CAGTATTAGA CAAACTTGGC CCTACGGTGT TTGCTCCTGT TAAAATGGAT 
CTTGTTGGAA ATATTAAGAA AGTAAATCAG AAGTATATAA CCAACAAGGA AGAGTTCACT 
ACCCTCCAGA AGATAGTGCT GCACGAAGTG GAGGCTGATG TAGCCCAGGT TAGGAACTCA 
GCTACAGAAG CCCTCTTGTG GCTGAAGAGA GGTCTCAAAT TTTTGAAGGG ATTTTTGACA 
GAAGTGAAAA ATGGTGAAAA GGATATCCAG ACAGCCTTGA ATAACGCATA TGGTAAAACA 
TTACGGCAGC ACCATGGCTG GGTAGTTCGA GGGGTTTTTG CGTTAGCTCT GAGGGCAGCT 
CCATCCTATG AAGATTTTGT CGCCGCATTA ACCATAAAGG AAGGCGACCA CCAGAAAGCA 
GCTTTCAGTG TTGGAATGCA GAGGGACCTC AGCCTTTACC TCCCTGCCAT GGAAAAGCAG 
CTGGCGATAC TGGACACTTT ATACGAGGTC CACGGACTGG AGTCTGATGA GGTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161170.1
CDS	315..1874
Translation	MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELNSCENGSLHMEINDGEEILMKNKSSLYLKPEIDCSISSEENTDDNITVQGEIMKEEGEDNLGNHDSSLAQPASDSSSSPPESHWEEGQEIIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKAAFSVGMQRDLSLYLPAMEKQLAILDTLYEVHGLESDEVV

Target ORF information:

RefSeq Version	NM_001167698.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167698.1

ORF Insert Sequence:

ATGGAGGGGG TGCTGTACAA GTGGACCAAC TATCTGAGCG GTTGGCAGCC TAGATGGTTC 
CTACTCTGTG GGGGTATATT ATCCTATTAT GATTCTCCTG AAGATGCCTG GAAAGGTTGC 
AAAGGGAGTA TCCAAATGGC GGTCTGTGAA ATTCAAGTTC ATTCTGTAGA TAACACACGC 
ATGGACCTGA TAATCCCTGG GGAGCAGTAT TTCTACTTGA AGGCCAGAAG TGTGGCTGAG 
AGACAGCGGT GGCTGGTAGC CCTGGGGTCA GCCAAGGCTT GCCTGACAGA TAGTAGGACC 
CAGAAGGAAA AAGAGTTTGC TGAAAACACT GAAAACTTGA AAACCAAAAT GTCGGAACTA 
AGACTCTACT GTGACCTCCT TGTTCAGCAA GTAGATAAAA CAAAAGAAGT AACCACAACT 
GGTGTGTCCA ATTCTGAGGA AGGAATTGAT GTGGGAACTC TGCTGAAATC AACCTGCAAT 
ACTTTTCTGA AGACTTTGGA AGAATGCATG CAGATCGCAA ACGCAGCCTT CACCTCTGAA 
CTACTCTATC GTACTCCCCC CGGCTCACCA CAGCTGGCCA TGCTCAAGTC CAGCAAGATG 
AAACATCCTA TTATACCGAT TCATAATTCA TTGGAAAGGC AAATGGAGTT AAACAGTTGT 
GAAAATGGAT CTTTGCATAT GGAAATAAAT GATGGTGAAG AAATCCTAAT GAAAAATAAG 
AGCTCCTTAT ATTTGAAACC AGAGATAGAT TGCAGCATAT CAAGTGAAGA AAATACAGAT 
GATAATATAA CAGTCCAAGG TGAGATCATG AAGGAAGAAG GAGAGGACAA CCTGGGAAAT 
CATGACAGCA GCTTAGCACA GCCTGCATCA GACTCCTCAA GTAGCCCTCC AGAATCCCAC 
TGGGAAGAAG GCCAAGAAAT CATCCCAACT TTCTTTAGTA CCATGAATAC CAGCTTTAGT 
GACATTGAAC TTCTGGAAGA CAGTGGCATT CCCACAGAAG CATTCTTGGC ATCATGTTAT 
GCTGTGGTTC CAGTATTAGA CAAACTTGGC CCTACGGTGT TTGCTCCTGT TAAAATGGAT 
CTTGTTGGAA ATATTAAGAA AGTAAATCAG AAGTATATAA CCAACAAGGA AGAGTTCACT 
ACCCTCCAGA AGATAGTGCT GCACGAAGTG GAGGCTGATG TAGCCCAGGT TAGGAACTCA 
GCTACAGAAG CCCTCTTGTG GCTGAAGAGA GGTCTCAAAT TTTTGAAGGG ATTTTTGACA 
GAAGTGAAAA ATGGTGAAAA GGATATCCAG ACAGCCTTGA ATAACGCATA TGGTAAAACA 
TTACGGCAGC ACCATGGCTG GGTAGTTCGA GGGGTTTTTG CGTTAGCTCT GAGGGCAGCT 
CCATCCTATG AAGATTTTGT CGCCGCATTA ACCATAAAGG AAGGCGACCA CCAGAAAGCA 
GCTTTCAGTG TTGGAATGCA GAGGGACCTC AGCCTTTACC TCCCTGCCAT GGAAAAGCAG 
CTGGCGATAC TGGACACTTT ATACGAGGTC CACGGACTGG AGTCTGATGA GGTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHA8 ( XM_022427144.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282852.1 Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe293085
Clone ID Related Accession (Same CDS sequence)	XM_022427144.1 , XM_022427145.1
Accession Version	XM_022427144.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology domain-containing family A member 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCT GTGAAATTCA AGTTCATTCT GTAGATAACA CACGCATGGA CCTGATAATC 
CCTGGGGAGC AGTATTTCTA CTTGAAGGCC AGAAGTGTGG CTGAGAGACA GCGGTGGCTG 
GTAGCCCTGG GGTCAGCCAA GGCTTGCCTG ACAGATAGTA GGACCCAGAA GGAAAAAGAG 
TTTGCTGAAA ACACTGAAAA CTTGAAAACC AAAATGTCGG AACTAAGACT CTACTGTGAC 
CTCCTTGTTC AGCAAGTAGA TAAAACAAAA GAAGTAACCA CAACTGGTGT GTCCAATTCT 
GAGGAAGGAA TTGATGTGGG AACTCTGCTG AAATCAACCT GCAATACTTT TCTGAAGACT 
TTGGAAGAAT GCATGCAGAT CGCAAACGCA GCCTTCACCT CTGAACTACT CTATCGTACT 
CCCCCCGGCT CACCACAGCT GGCCATGCTC AAGTCCAGCA AGATGAAACA TCCTATTATA 
CCGATTCATA ATTCATTGGA AAGGCAAATG GAGTTAAACA GTTGTGAAAA TGGATCTTTG 
CATATGGAAA TAAATGATGG TGAAGAAATC CTAATGAAAA ATAAGAGCTC CTTATATTTG 
AAACCAGAGA TAGATTGCAG CATATCAAGT GAAGAAAATA CAGATGATAA TATAACAGTC 
CAAGGTGAGA TCATGAAGGA AGAAGGAGAG GACAACCTGG GAAATCATGA CAGCAGCTTA 
GCACAGCCTG CATCAGACTC CTCAAGTAGC CCTCCAGAAT CCCACTGGGA AGAAGGCCAA 
GAAATCATCC CAACTTTCTT TAGTACCATG AATACCAGCT TTAGTGACAT TGAACTTCTG 
GAAGACAGTG GCATTCCCAC AGAAGCATTC TTGGCATCAT GTTATGCTGT GGTTCCAGTA 
TTAGACAAAC TTGGCCCTAC GGTGTTTGCT CCTGTTAAAA TGGATCTTGT TGGAAATATT 
AAGAAAGTAA ATCAGAAGTA TATAACCAAC AAGGAAGAGT TCACTACCCT CCAGAAGATA 
GTGCTGCACG AAGTGGAGGC TGATGTAGCC CAGGTTAGGA ACTCAGCTAC AGAAGCCCTC 
TTGTGGCTGA AGAGAGGTCT CAAATTTTTG AAGGGATTTT TGACAGAAGT GAAAAATGGT 
GAAAAGGATA TCCAGACAGC CTTGAATAAC GCATATGGTA AAACATTACG GCAGCACCAT 
GGCTGGGTAG TTCGAGGGGT TTTTGCGTTA GCTCTGAGGG CAGCTCCATC CTATGAAGAT 
TTTGTCGCCG CATTAACCAT AAAGGAAGGC GACCACCAGA AAGCAGCTTT CAGTGTTGGA 
ATGCAGAGGG ACCTCAGCCT TTACCTCCCT GCCATGGAAA AGCAGCTGGC GATACTGGAC 
ACTTTATACG AGGTCCACGG ACTGGAGTCT GATGAGGTGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282852.1
CDS	264..1688
Translation	MAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELNSCENGSLHMEINDGEEILMKNKSSLYLKPEIDCSISSEENTDDNITVQGEIMKEEGEDNLGNHDSSLAQPASDSSSSPPESHWEEGQEIIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKAAFSVGMQRDLSLYLPAMEKQLAILDTLYEVHGLESDEVV

Target ORF information:

RefSeq Version	XM_022427144.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022427144.1

ORF Insert Sequence:

ATGGCGGTCT GTGAAATTCA AGTTCATTCT GTAGATAACA CACGCATGGA CCTGATAATC 
CCTGGGGAGC AGTATTTCTA CTTGAAGGCC AGAAGTGTGG CTGAGAGACA GCGGTGGCTG 
GTAGCCCTGG GGTCAGCCAA GGCTTGCCTG ACAGATAGTA GGACCCAGAA GGAAAAAGAG 
TTTGCTGAAA ACACTGAAAA CTTGAAAACC AAAATGTCGG AACTAAGACT CTACTGTGAC 
CTCCTTGTTC AGCAAGTAGA TAAAACAAAA GAAGTAACCA CAACTGGTGT GTCCAATTCT 
GAGGAAGGAA TTGATGTGGG AACTCTGCTG AAATCAACCT GCAATACTTT TCTGAAGACT 
TTGGAAGAAT GCATGCAGAT CGCAAACGCA GCCTTCACCT CTGAACTACT CTATCGTACT 
CCCCCCGGCT CACCACAGCT GGCCATGCTC AAGTCCAGCA AGATGAAACA TCCTATTATA 
CCGATTCATA ATTCATTGGA AAGGCAAATG GAGTTAAACA GTTGTGAAAA TGGATCTTTG 
CATATGGAAA TAAATGATGG TGAAGAAATC CTAATGAAAA ATAAGAGCTC CTTATATTTG 
AAACCAGAGA TAGATTGCAG CATATCAAGT GAAGAAAATA CAGATGATAA TATAACAGTC 
CAAGGTGAGA TCATGAAGGA AGAAGGAGAG GACAACCTGG GAAATCATGA CAGCAGCTTA 
GCACAGCCTG CATCAGACTC CTCAAGTAGC CCTCCAGAAT CCCACTGGGA AGAAGGCCAA 
GAAATCATCC CAACTTTCTT TAGTACCATG AATACCAGCT TTAGTGACAT TGAACTTCTG 
GAAGACAGTG GCATTCCCAC AGAAGCATTC TTGGCATCAT GTTATGCTGT GGTTCCAGTA 
TTAGACAAAC TTGGCCCTAC GGTGTTTGCT CCTGTTAAAA TGGATCTTGT TGGAAATATT 
AAGAAAGTAA ATCAGAAGTA TATAACCAAC AAGGAAGAGT TCACTACCCT CCAGAAGATA 
GTGCTGCACG AAGTGGAGGC TGATGTAGCC CAGGTTAGGA ACTCAGCTAC AGAAGCCCTC 
TTGTGGCTGA AGAGAGGTCT CAAATTTTTG AAGGGATTTT TGACAGAAGT GAAAAATGGT 
GAAAAGGATA TCCAGACAGC CTTGAATAAC GCATATGGTA AAACATTACG GCAGCACCAT 
GGCTGGGTAG TTCGAGGGGT TTTTGCGTTA GCTCTGAGGG CAGCTCCATC CTATGAAGAT 
TTTGTCGCCG CATTAACCAT AAAGGAAGGC GACCACCAGA AAGCAGCTTT CAGTGTTGGA 
ATGCAGAGGG ACCTCAGCCT TTACCTCCCT GCCATGGAAA AGCAGCTGGC GATACTGGAC 
ACTTTATACG AGGTCCACGG ACTGGAGTCT GATGAGGTGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHA8 ( XM_022427145.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282853.1 Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe293085
Clone ID Related Accession (Same CDS sequence)	XM_022427144.1 , XM_022427145.1
Accession Version	XM_022427145.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology domain-containing family A member 8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTCT GTGAAATTCA AGTTCATTCT GTAGATAACA CACGCATGGA CCTGATAATC 
CCTGGGGAGC AGTATTTCTA CTTGAAGGCC AGAAGTGTGG CTGAGAGACA GCGGTGGCTG 
GTAGCCCTGG GGTCAGCCAA GGCTTGCCTG ACAGATAGTA GGACCCAGAA GGAAAAAGAG 
TTTGCTGAAA ACACTGAAAA CTTGAAAACC AAAATGTCGG AACTAAGACT CTACTGTGAC 
CTCCTTGTTC AGCAAGTAGA TAAAACAAAA GAAGTAACCA CAACTGGTGT GTCCAATTCT 
GAGGAAGGAA TTGATGTGGG AACTCTGCTG AAATCAACCT GCAATACTTT TCTGAAGACT 
TTGGAAGAAT GCATGCAGAT CGCAAACGCA GCCTTCACCT CTGAACTACT CTATCGTACT 
CCCCCCGGCT CACCACAGCT GGCCATGCTC AAGTCCAGCA AGATGAAACA TCCTATTATA 
CCGATTCATA ATTCATTGGA AAGGCAAATG GAGTTAAACA GTTGTGAAAA TGGATCTTTG 
CATATGGAAA TAAATGATGG TGAAGAAATC CTAATGAAAA ATAAGAGCTC CTTATATTTG 
AAACCAGAGA TAGATTGCAG CATATCAAGT GAAGAAAATA CAGATGATAA TATAACAGTC 
CAAGGTGAGA TCATGAAGGA AGAAGGAGAG GACAACCTGG GAAATCATGA CAGCAGCTTA 
GCACAGCCTG CATCAGACTC CTCAAGTAGC CCTCCAGAAT CCCACTGGGA AGAAGGCCAA 
GAAATCATCC CAACTTTCTT TAGTACCATG AATACCAGCT TTAGTGACAT TGAACTTCTG 
GAAGACAGTG GCATTCCCAC AGAAGCATTC TTGGCATCAT GTTATGCTGT GGTTCCAGTA 
TTAGACAAAC TTGGCCCTAC GGTGTTTGCT CCTGTTAAAA TGGATCTTGT TGGAAATATT 
AAGAAAGTAA ATCAGAAGTA TATAACCAAC AAGGAAGAGT TCACTACCCT CCAGAAGATA 
GTGCTGCACG AAGTGGAGGC TGATGTAGCC CAGGTTAGGA ACTCAGCTAC AGAAGCCCTC 
TTGTGGCTGA AGAGAGGTCT CAAATTTTTG AAGGGATTTT TGACAGAAGT GAAAAATGGT 
GAAAAGGATA TCCAGACAGC CTTGAATAAC GCATATGGTA AAACATTACG GCAGCACCAT 
GGCTGGGTAG TTCGAGGGGT TTTTGCGTTA GCTCTGAGGG CAGCTCCATC CTATGAAGAT 
TTTGTCGCCG CATTAACCAT AAAGGAAGGC GACCACCAGA AAGCAGCTTT CAGTGTTGGA 
ATGCAGAGGG ACCTCAGCCT TTACCTCCCT GCCATGGAAA AGCAGCTGGC GATACTGGAC 
ACTTTATACG AGGTCCACGG ACTGGAGTCT GATGAGGTGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282853.1
CDS	246..1670
Translation	MAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELNSCENGSLHMEINDGEEILMKNKSSLYLKPEIDCSISSEENTDDNITVQGEIMKEEGEDNLGNHDSSLAQPASDSSSSPPESHWEEGQEIIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKAAFSVGMQRDLSLYLPAMEKQLAILDTLYEVHGLESDEVV

Target ORF information:

RefSeq Version	XM_022427145.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022427145.1

ORF Insert Sequence:

ATGGCGGTCT GTGAAATTCA AGTTCATTCT GTAGATAACA CACGCATGGA CCTGATAATC 
CCTGGGGAGC AGTATTTCTA CTTGAAGGCC AGAAGTGTGG CTGAGAGACA GCGGTGGCTG 
GTAGCCCTGG GGTCAGCCAA GGCTTGCCTG ACAGATAGTA GGACCCAGAA GGAAAAAGAG 
TTTGCTGAAA ACACTGAAAA CTTGAAAACC AAAATGTCGG AACTAAGACT CTACTGTGAC 
CTCCTTGTTC AGCAAGTAGA TAAAACAAAA GAAGTAACCA CAACTGGTGT GTCCAATTCT 
GAGGAAGGAA TTGATGTGGG AACTCTGCTG AAATCAACCT GCAATACTTT TCTGAAGACT 
TTGGAAGAAT GCATGCAGAT CGCAAACGCA GCCTTCACCT CTGAACTACT CTATCGTACT 
CCCCCCGGCT CACCACAGCT GGCCATGCTC AAGTCCAGCA AGATGAAACA TCCTATTATA 
CCGATTCATA ATTCATTGGA AAGGCAAATG GAGTTAAACA GTTGTGAAAA TGGATCTTTG 
CATATGGAAA TAAATGATGG TGAAGAAATC CTAATGAAAA ATAAGAGCTC CTTATATTTG 
AAACCAGAGA TAGATTGCAG CATATCAAGT GAAGAAAATA CAGATGATAA TATAACAGTC 
CAAGGTGAGA TCATGAAGGA AGAAGGAGAG GACAACCTGG GAAATCATGA CAGCAGCTTA 
GCACAGCCTG CATCAGACTC CTCAAGTAGC CCTCCAGAAT CCCACTGGGA AGAAGGCCAA 
GAAATCATCC CAACTTTCTT TAGTACCATG AATACCAGCT TTAGTGACAT TGAACTTCTG 
GAAGACAGTG GCATTCCCAC AGAAGCATTC TTGGCATCAT GTTATGCTGT GGTTCCAGTA 
TTAGACAAAC TTGGCCCTAC GGTGTTTGCT CCTGTTAAAA TGGATCTTGT TGGAAATATT 
AAGAAAGTAA ATCAGAAGTA TATAACCAAC AAGGAAGAGT TCACTACCCT CCAGAAGATA 
GTGCTGCACG AAGTGGAGGC TGATGTAGCC CAGGTTAGGA ACTCAGCTAC AGAAGCCCTC 
TTGTGGCTGA AGAGAGGTCT CAAATTTTTG AAGGGATTTT TGACAGAAGT GAAAAATGGT 
GAAAAGGATA TCCAGACAGC CTTGAATAAC GCATATGGTA AAACATTACG GCAGCACCAT 
GGCTGGGTAG TTCGAGGGGT TTTTGCGTTA GCTCTGAGGG CAGCTCCATC CTATGAAGAT 
TTTGTCGCCG CATTAACCAT AAAGGAAGGC GACCACCAGA AAGCAGCTTT CAGTGTTGGA 
ATGCAGAGGG ACCTCAGCCT TTACCTCCCT GCCATGGAAA AGCAGCTGGC GATACTGGAC 
ACTTTATACG AGGTCCACGG ACTGGAGTCT GATGAGGTGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHA8 ( XM_022427142.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282850.1 Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe293083
Clone ID Related Accession (Same CDS sequence)	XM_022427142.1
Accession Version	XM_022427142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology domain-containing family A member 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCCAG CTGAGGACAT TGACAAAACT TATGCAGAAA TAGTTAGAGA TGACATTTTA 
GAAAGAAGAT TCAACTTAGG CAAAAGCCCA GAAGCAAGAA TACAGAAAAT AAGTTTGGTT 
TGTCTAGAAT ATAGATTGGG TTGGCAGCCT AGATGGTTCC TACTCTGTGG GGGTATATTA 
TCCTATTATG ATTCTCCTGA AGATGCCTGG AAAGGTTGCA AAGGGAGTAT CCAAATGGCG 
GTCTGTGAAA TTCAAGTTCA TTCTGTAGAT AACACACGCA TGGACCTGAT AATCCCTGGG 
GAGCAGTATT TCTACTTGAA GGCCAGAAGT GTGGCTGAGA GACAGCGGTG GCTGGTAGCC 
CTGGGGTCAG CCAAGGCTTG CCTGACAGAT AGTAGGACCC AGAAGGAAAA AGAGTTTGCT 
GAAAACACTG AAAACTTGAA AACCAAAATG TCGGAACTAA GACTCTACTG TGACCTCCTT 
GTTCAGCAAG TAGATAAAAC AAAAGAAGTA ACCACAACTG GTGTGTCCAA TTCTGAGGAA 
GGAATTGATG TGGGAACTCT GCTGAAATCA ACCTGCAATA CTTTTCTGAA GACTTTGGAA 
GAATGCATGC AGATCGCAAA CGCAGCCTTC ACCTCTGAAC TACTCTATCG TACTCCCCCC 
GGCTCACCAC AGCTGGCCAT GCTCAAGTCC AGCAAGATGA AACATCCTAT TATACCGATT 
CATAATTCAT TGGAAAGGCA AATGGAGTTA AACAGTTGTG AAAATGGATC TTTGCATATG 
GAAATAAATG ATGGTGAAGA AATCCTAATG AAAAATAAGA GCTCCTTATA TTTGAAACCA 
GAGATAGATT GCAGCATATC AAGTGAAGAA AATACAGATG ATAATATAAC AGTCCAAGGT 
GAGATCATGA AGGAAGAAGG AGAGGACAAC CTGGGAAATC ATGACAGCAG CTTAGCACAG 
CCTGCATCAG ACTCCTCAAG TAGCCCTCCA GAATCCCACT GGGAAGAAGG CCAAGAAATC 
ATCCCAACTT TCTTTAGTAC CATGAATACC AGCTTTAGTG ACATTGAACT TCTGGAAGAC 
AGTGGCATTC CCACAGAAGC ATTCTTGGCA TCATGTTATG CTGTGGTTCC AGTATTAGAC 
AAACTTGGCC CTACGGTGTT TGCTCCTGTT AAAATGGATC TTGTTGGAAA TATTAAGAAA 
GTAAATCAGA AGTATATAAC CAACAAGGAA GAGTTCACTA CCCTCCAGAA GATAGTGCTG 
CACGAAGTGG AGGCTGATGT AGCCCAGGTT AGGAACTCAG CTACAGAAGC CCTCTTGTGG 
CTGAAGAGAG GTCTCAAATT TTTGAAGGGA TTTTTGACAG AAGTGAAAAA TGGTGAAAAG 
GATATCCAGA CAGCCTTGAA TAACGCATAT GGTAAAACAT TACGGCAGCA CCATGGCTGG 
GTAGTTCGAG GGGTTTTTGC GTTAGCTCTG AGGGCAGCTC CATCCTATGA AGATTTTGTC 
GCCGCATTAA CCATAAAGGA AGGCGACCAC CAGAAAGCAG CTTTCAGTGT TGGAATGCAG 
AGGGACCTCA GCCTTTACCT CCCTGCCATG GAAAAGCAGC TGGCGATACT GGACACTTTA 
TACGAGGTCC ACGGACTGGA GTCTGATGAG GTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282850.1
CDS	16..1674
Translation	MYPAEDIDKTYAEIVRDDILERRFNLGKSPEARIQKISLVCLEYRLGWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELNSCENGSLHMEINDGEEILMKNKSSLYLKPEIDCSISSEENTDDNITVQGEIMKEEGEDNLGNHDSSLAQPASDSSSSPPESHWEEGQEIIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKAAFSVGMQRDLSLYLPAMEKQLAILDTLYEVHGLESDEVV

Target ORF information:

RefSeq Version	XM_022427142.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022427142.1

ORF Insert Sequence:

ATGTATCCAG CTGAGGACAT TGACAAAACT TATGCAGAAA TAGTTAGAGA TGACATTTTA 
GAAAGAAGAT TCAACTTAGG CAAAAGCCCA GAAGCAAGAA TACAGAAAAT AAGTTTGGTT 
TGTCTAGAAT ATAGATTGGG TTGGCAGCCT AGATGGTTCC TACTCTGTGG GGGTATATTA 
TCCTATTATG ATTCTCCTGA AGATGCCTGG AAAGGTTGCA AAGGGAGTAT CCAAATGGCG 
GTCTGTGAAA TTCAAGTTCA TTCTGTAGAT AACACACGCA TGGACCTGAT AATCCCTGGG 
GAGCAGTATT TCTACTTGAA GGCCAGAAGT GTGGCTGAGA GACAGCGGTG GCTGGTAGCC 
CTGGGGTCAG CCAAGGCTTG CCTGACAGAT AGTAGGACCC AGAAGGAAAA AGAGTTTGCT 
GAAAACACTG AAAACTTGAA AACCAAAATG TCGGAACTAA GACTCTACTG TGACCTCCTT 
GTTCAGCAAG TAGATAAAAC AAAAGAAGTA ACCACAACTG GTGTGTCCAA TTCTGAGGAA 
GGAATTGATG TGGGAACTCT GCTGAAATCA ACCTGCAATA CTTTTCTGAA GACTTTGGAA 
GAATGCATGC AGATCGCAAA CGCAGCCTTC ACCTCTGAAC TACTCTATCG TACTCCCCCC 
GGCTCACCAC AGCTGGCCAT GCTCAAGTCC AGCAAGATGA AACATCCTAT TATACCGATT 
CATAATTCAT TGGAAAGGCA AATGGAGTTA AACAGTTGTG AAAATGGATC TTTGCATATG 
GAAATAAATG ATGGTGAAGA AATCCTAATG AAAAATAAGA GCTCCTTATA TTTGAAACCA 
GAGATAGATT GCAGCATATC AAGTGAAGAA AATACAGATG ATAATATAAC AGTCCAAGGT 
GAGATCATGA AGGAAGAAGG AGAGGACAAC CTGGGAAATC ATGACAGCAG CTTAGCACAG 
CCTGCATCAG ACTCCTCAAG TAGCCCTCCA GAATCCCACT GGGAAGAAGG CCAAGAAATC 
ATCCCAACTT TCTTTAGTAC CATGAATACC AGCTTTAGTG ACATTGAACT TCTGGAAGAC 
AGTGGCATTC CCACAGAAGC ATTCTTGGCA TCATGTTATG CTGTGGTTCC AGTATTAGAC 
AAACTTGGCC CTACGGTGTT TGCTCCTGTT AAAATGGATC TTGTTGGAAA TATTAAGAAA 
GTAAATCAGA AGTATATAAC CAACAAGGAA GAGTTCACTA CCCTCCAGAA GATAGTGCTG 
CACGAAGTGG AGGCTGATGT AGCCCAGGTT AGGAACTCAG CTACAGAAGC CCTCTTGTGG 
CTGAAGAGAG GTCTCAAATT TTTGAAGGGA TTTTTGACAG AAGTGAAAAA TGGTGAAAAG 
GATATCCAGA CAGCCTTGAA TAACGCATAT GGTAAAACAT TACGGCAGCA CCATGGCTGG 
GTAGTTCGAG GGGTTTTTGC GTTAGCTCTG AGGGCAGCTC CATCCTATGA AGATTTTGTC 
GCCGCATTAA CCATAAAGGA AGGCGACCAC CAGAAAGCAG CTTTCAGTGT TGGAATGCAG 
AGGGACCTCA GCCTTTACCT CCCTGCCATG GAAAAGCAGC TGGCGATACT GGACACTTTA 
TACGAGGTCC ACGGACTGGA GTCTGATGAG GTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLEKHA8 ( XM_022427143.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022282851.1 Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe293084
Clone ID Related Accession (Same CDS sequence)	XM_022427143.1
Accession Version	XM_022427143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	pleckstrin homology domain-containing family A member 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGTT GGCAGCCTAG ATGGTTCCTA CTCTGTGGGG GTATATTATC CTATTATGAT 
TCTCCTGAAG ATGCCTGGAA AGGTTGCAAA GGGAGTATCC AAATGGCGGT CTGTGAAATT 
CAAGTTCATT CTGTAGATAA CACACGCATG GACCTGATAA TCCCTGGGGA GCAGTATTTC 
TACTTGAAGG CCAGAAGTGT GGCTGAGAGA CAGCGGTGGC TGGTAGCCCT GGGGTCAGCC 
AAGGCTTGCC TGACAGATAG TAGGACCCAG AAGGAAAAAG AGTTTGCTGA AAACACTGAA 
AACTTGAAAA CCAAAATGTC GGAACTAAGA CTCTACTGTG ACCTCCTTGT TCAGCAAGTA 
GATAAAACAA AAGAAGTAAC CACAACTGGT GTGTCCAATT CTGAGGAAGG AATTGATGTG 
GGAACTCTGC TGAAATCAAC CTGCAATACT TTTCTGAAGA CTTTGGAAGA ATGCATGCAG 
ATCGCAAACG CAGCCTTCAC CTCTGAACTA CTCTATCGTA CTCCCCCCGG CTCACCACAG 
CTGGCCATGC TCAAGTCCAG CAAGATGAAA CATCCTATTA TACCGATTCA TAATTCATTG 
GAAAGGCAAA TGGAGTTAAA CAGTTGTGAA AATGGATCTT TGCATATGGA AATAAATGAT 
GGTGAAGAAA TCCTAATGAA AAATAAGAGC TCCTTATATT TGAAACCAGA GATAGATTGC 
AGCATATCAA GTGAAGAAAA TACAGATGAT AATATAACAG TCCAAGGTGA GATCATGAAG 
GAAGAAGGAG AGGACAACCT GGGAAATCAT GACAGCAGCT TAGCACAGCC TGCATCAGAC 
TCCTCAAGTA GCCCTCCAGA ATCCCACTGG GAAGAAGGCC AAGAAATCAT CCCAACTTTC 
TTTAGTACCA TGAATACCAG CTTTAGTGAC ATTGAACTTC TGGAAGACAG TGGCATTCCC 
ACAGAAGCAT TCTTGGCATC ATGTTATGCT GTGGTTCCAG TATTAGACAA ACTTGGCCCT 
ACGGTGTTTG CTCCTGTTAA AATGGATCTT GTTGGAAATA TTAAGAAAGT AAATCAGAAG 
TATATAACCA ACAAGGAAGA GTTCACTACC CTCCAGAAGA TAGTGCTGCA CGAAGTGGAG 
GCTGATGTAG CCCAGGTTAG GAACTCAGCT ACAGAAGCCC TCTTGTGGCT GAAGAGAGGT 
CTCAAATTTT TGAAGGGATT TTTGACAGAA GTGAAAAATG GTGAAAAGGA TATCCAGACA 
GCCTTGAATA ACGCATATGG TAAAACATTA CGGCAGCACC ATGGCTGGGT AGTTCGAGGG 
GTTTTTGCGT TAGCTCTGAG GGCAGCTCCA TCCTATGAAG ATTTTGTCGC CGCATTAACC 
ATAAAGGAAG GCGACCACCA GAAAGCAGCT TTCAGTGTTG GAATGCAGAG GGACCTCAGC 
CTTTACCTCC CTGCCATGGA AAAGCAGCTG GCGATACTGG ACACTTTATA CGAGGTCCAC 
GGACTGGAGT CTGATGAGGT GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022282851.1
CDS	16..1542
Translation	MQSWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQYFYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTTGVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNSLERQMELNSCENGSLHMEINDGEEILMKNKSSLYLKPEIDCSISSEENTDDNITVQGEIMKEEGEDNLGNHDSSLAQPASDSSSSPPESHWEEGQEIIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLGPTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLTEVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFALALRAAPSYEDFVAALTIKEGDHQKAAFSVGMQRDLSLYLPAMEKQLAILDTLYEVHGLESDEVV

Target ORF information:

RefSeq Version	XM_022427143.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris pleckstrin homology domain containing A8 (PLEKHA8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022427143.1

ORF Insert Sequence:

ATGCAAAGTT GGCAGCCTAG ATGGTTCCTA CTCTGTGGGG GTATATTATC CTATTATGAT 
TCTCCTGAAG ATGCCTGGAA AGGTTGCAAA GGGAGTATCC AAATGGCGGT CTGTGAAATT 
CAAGTTCATT CTGTAGATAA CACACGCATG GACCTGATAA TCCCTGGGGA GCAGTATTTC 
TACTTGAAGG CCAGAAGTGT GGCTGAGAGA CAGCGGTGGC TGGTAGCCCT GGGGTCAGCC 
AAGGCTTGCC TGACAGATAG TAGGACCCAG AAGGAAAAAG AGTTTGCTGA AAACACTGAA 
AACTTGAAAA CCAAAATGTC GGAACTAAGA CTCTACTGTG ACCTCCTTGT TCAGCAAGTA 
GATAAAACAA AAGAAGTAAC CACAACTGGT GTGTCCAATT CTGAGGAAGG AATTGATGTG 
GGAACTCTGC TGAAATCAAC CTGCAATACT TTTCTGAAGA CTTTGGAAGA ATGCATGCAG 
ATCGCAAACG CAGCCTTCAC CTCTGAACTA CTCTATCGTA CTCCCCCCGG CTCACCACAG 
CTGGCCATGC TCAAGTCCAG CAAGATGAAA CATCCTATTA TACCGATTCA TAATTCATTG 
GAAAGGCAAA TGGAGTTAAA CAGTTGTGAA AATGGATCTT TGCATATGGA AATAAATGAT 
GGTGAAGAAA TCCTAATGAA AAATAAGAGC TCCTTATATT TGAAACCAGA GATAGATTGC 
AGCATATCAA GTGAAGAAAA TACAGATGAT AATATAACAG TCCAAGGTGA GATCATGAAG 
GAAGAAGGAG AGGACAACCT GGGAAATCAT GACAGCAGCT TAGCACAGCC TGCATCAGAC 
TCCTCAAGTA GCCCTCCAGA ATCCCACTGG GAAGAAGGCC AAGAAATCAT CCCAACTTTC 
TTTAGTACCA TGAATACCAG CTTTAGTGAC ATTGAACTTC TGGAAGACAG TGGCATTCCC 
ACAGAAGCAT TCTTGGCATC ATGTTATGCT GTGGTTCCAG TATTAGACAA ACTTGGCCCT 
ACGGTGTTTG CTCCTGTTAA AATGGATCTT GTTGGAAATA TTAAGAAAGT AAATCAGAAG 
TATATAACCA ACAAGGAAGA GTTCACTACC CTCCAGAAGA TAGTGCTGCA CGAAGTGGAG 
GCTGATGTAG CCCAGGTTAG GAACTCAGCT ACAGAAGCCC TCTTGTGGCT GAAGAGAGGT 
CTCAAATTTT TGAAGGGATT TTTGACAGAA GTGAAAAATG GTGAAAAGGA TATCCAGACA 
GCCTTGAATA ACGCATATGG TAAAACATTA CGGCAGCACC ATGGCTGGGT AGTTCGAGGG 
GTTTTTGCGT TAGCTCTGAG GGCAGCTCCA TCCTATGAAG ATTTTGTCGC CGCATTAACC 
ATAAAGGAAG GCGACCACCA GAAAGCAGCT TTCAGTGTTG GAATGCAGAG GGACCTCAGC 
CTTTACCTCC CTGCCATGGA AAAGCAGCTG GCGATACTGG ACACTTTATA CGAGGTCCAC 
GGACTGGAGT CTGATGAGGT GGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FAPP2 is required for aquaporin-2 apical sorting at trans-Golgi network in polarized MDCK cells.
American journal of physiology. Cell physiology297(6)C1389-96(2009 Dec)
Yui N,Okutsu R,Sohara E,Rai T,Ohta A,Noda Y,Sasaki S,Uchida S

Golgi protein FAPP2 tubulates membranes.
Proceedings of the National Academy of Sciences of the United States of America106(50)21121-5(2009 Dec)
Cao X,Coskun U,R?ssle M,Buschhorn SB,Grzybek M,Dafforn TR,Lenoir M,Overduin M,Simons K

Genome sequence, comparative analysis and haplotype structure of the domestic dog.
Nature438(7069)803-19(2005 Dec)
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FAPP2 is involved in the transport of apical cargo in polarized MDCK cells.
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Vieira OV,Verkade P,Manninen A,Simons K

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PLEKHA8 cDNA ORF clone, Canis lupus familiaris(dog)

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Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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