VWDE cDNA ORF clone, Canis lupus familiaris(dog)

The following VWDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe293244 XM_022427414.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe293245 XM_022427415.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe293246 XM_022427416.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe293247 XM_022427417.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe293248 XM_022427418.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe40041 XM_014119190.2
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40042 XM_014119191.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40043 XM_014119192.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe40044 XM_014119193.1
Latest version!
Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
View Old Accession Versions >>
OCe40041 XM_014119190.1 Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe293244
    Clone ID Related Accession (Same CDS sequence) XM_022427414.1
    Accession Version XM_022427414.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGGTAG TATGAAAATT CTTCAGAAGA TGGGAAGAAA ACTATTTGGT
    TCACATTTAA CCATTTGTAG ACACCCATGT GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC
    ATATGCAAAT GTAAACCTGG TTACACTGGT TCTAACTGCC AAACTGCTGT ATGTCATCCT
    GATTGCAAAA ACCATGGAAA ATGTATCAAG CCTAACATTT GTGACTGTCC CCCGGGACAT
    GGGGGTGCAA CCTGTGATGA AGAACATTGT AGCCCACCTT GTCAACATGG TGGCACCTGC
    TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC GGTTTTATAG GACCAAGGTG TGAAACAATG
    GTTTGTAACA GGCACTGTGA AAATGGAGGT GAATGTCTTA CACCAGATAT TTGCCAGTGC
    AAGCCTGGCT GGTATGGACC TACTTGTGGT ACAGCTTTGT GTGACCCCGT TTGCCTCAAT
    GGTGGTTCCT GTAATAAGCC AAACACTTGC CTCTGTCCAA ATGGATTCTT TGGTGCACAG
    TGTCAGAATG CTATCTGTCA CCCTCCCTGT AAGAATGGTG GCCGCTGCAT GAGAAATAAT
    GTGTGTGCCT GTCGTGAAGG ATATGCTGGG AAGAGATGCC AAAAAAGTAT CTGTGATCCC
    ATGTGCATGA ATGGAGGAAA ATGCGTGGGA CCAAACATTT GCTCTTGTCC TTCCGGTTGG
    AGAGGGAAAC AATGCAACAT AGCTCTCTGC TTTCAGAAAT GTAAAAATGG TGGCGAATGT
    ATTGCTCCCA GTATATGCCA TTGTCCTACC ACATGGGAAG GAGGGCAGTG TCAAATACCA
    ATTTGCAAAC AGAAGTGTCT TTATGGAGGG AGATGTGTAT TTCCCAATGT GTGCTCCTGT
    CGCAATGGAT ATTCTGGAGT CAAATGTGAA AAGAAAGTAC AGGATGCTGA GCCCAGGATG
    CATCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283122.1
    CDS184..5592
    Translation

    Target ORF information:

    RefSeq Version XM_022427414.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427414.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGGTAG TATGAAAATT CTTCAGAAGA TGGGAAGAAA ACTATTTGGT
    TCACATTTAA CCATTTGTAG ACACCCATGT GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC
    ATATGCAAAT GTAAACCTGG TTACACTGGT TCTAACTGCC AAACTGCTGT ATGTCATCCT
    GATTGCAAAA ACCATGGAAA ATGTATCAAG CCTAACATTT GTGACTGTCC CCCGGGACAT
    GGGGGTGCAA CCTGTGATGA AGAACATTGT AGCCCACCTT GTCAACATGG TGGCACCTGC
    TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC GGTTTTATAG GACCAAGGTG TGAAACAATG
    GTTTGTAACA GGCACTGTGA AAATGGAGGT GAATGTCTTA CACCAGATAT TTGCCAGTGC
    AAGCCTGGCT GGTATGGACC TACTTGTGGT ACAGCTTTGT GTGACCCCGT TTGCCTCAAT
    GGTGGTTCCT GTAATAAGCC AAACACTTGC CTCTGTCCAA ATGGATTCTT TGGTGCACAG
    TGTCAGAATG CTATCTGTCA CCCTCCCTGT AAGAATGGTG GCCGCTGCAT GAGAAATAAT
    GTGTGTGCCT GTCGTGAAGG ATATGCTGGG AAGAGATGCC AAAAAAGTAT CTGTGATCCC
    ATGTGCATGA ATGGAGGAAA ATGCGTGGGA CCAAACATTT GCTCTTGTCC TTCCGGTTGG
    AGAGGGAAAC AATGCAACAT AGCTCTCTGC TTTCAGAAAT GTAAAAATGG TGGCGAATGT
    ATTGCTCCCA GTATATGCCA TTGTCCTACC ACATGGGAAG GAGGGCAGTG TCAAATACCA
    ATTTGCAAAC AGAAGTGTCT TTATGGAGGG AGATGTGTAT TTCCCAATGT GTGCTCCTGT
    CGCAATGGAT ATTCTGGAGT CAAATGTGAA AAGAAAGTAC AGGATGCTGA GCCCAGGATG
    CATCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293245
    Clone ID Related Accession (Same CDS sequence) XM_022427415.1
    Accession Version XM_022427415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGGTAG TATGAAAATT CTTCAGAAGA TGGGAAGAAA ACTATTTGGT
    TCACATTTAA CCATTTGTAG ACACCCATGT GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC
    ATATGCAAAT GTAAACCTGG TTACACTGGT TCTAACTGCC AAACTGCTGT ATGTCATCCT
    GATTGCAAAA ACCATGGAAA ATGTATCAAG CCTAACATTT GTGACTGTCC CCCGGGACAT
    GGGGGTGCAA CCTGTGATGA AGAACATTGT AGCCCACCTT GTCAACATGG TGGCACCTGC
    TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC GGTTTTATAG GACCAAGGTG TGAAACAATG
    GTTTGTAACA GGCACTGTGA AAATGGAGGT GAATGTCTTA CACCAGATAT TTGCCAGTGC
    AAGCCTGGCT GGTATGGACC TACTTGTGGT ACAGCTTTGT GTGACCCCGT TTGCCTCAAT
    GGTGGTTCCT GTAATAAGCC AAACACTTGC CTCTGTCCAA ATGGATTCTT TGGTGCACAG
    TGTCAGAATG CTATCTGTCA CCCTCCCTGT AAGAATGGTG GCCGCTGCAT GAGAAATAAT
    GTGTGTGCCT GTCGTGAAGG ATATGCTGGG AAGAGATGCC AAAAAAGTAT CTGTGATCCC
    ATGTGCATGA ATGGAGGAAA ATGCGTGGGA CCAAACATTT GCTCTTGTCC TTCCGGTTGG
    AGAGGGAAAC AATGCAACAT AGCTCTCTGC TTTCAGAAAT GTAAAAATGG TGGCGAATGT
    ATTGCTCCCA GTATATGCCA TTGTCCTACC ACATGGGAAG GAGGGCAGTG TCAAATACCA
    ATTTGCAAAC AGAAGTGTCT TTATGGAGGG AGATGTGTAT TTCCCAATGT GTGCTCCTGT
    CGCAATGGAT ATTCTGGAGT CAAATGTGAA AAGAAAGTAC AGACAAAACG CCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283123.1
    CDS184..5580
    Translation

    Target ORF information:

    RefSeq Version XM_022427415.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGGTAG TATGAAAATT CTTCAGAAGA TGGGAAGAAA ACTATTTGGT
    TCACATTTAA CCATTTGTAG ACACCCATGT GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC
    ATATGCAAAT GTAAACCTGG TTACACTGGT TCTAACTGCC AAACTGCTGT ATGTCATCCT
    GATTGCAAAA ACCATGGAAA ATGTATCAAG CCTAACATTT GTGACTGTCC CCCGGGACAT
    GGGGGTGCAA CCTGTGATGA AGAACATTGT AGCCCACCTT GTCAACATGG TGGCACCTGC
    TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC GGTTTTATAG GACCAAGGTG TGAAACAATG
    GTTTGTAACA GGCACTGTGA AAATGGAGGT GAATGTCTTA CACCAGATAT TTGCCAGTGC
    AAGCCTGGCT GGTATGGACC TACTTGTGGT ACAGCTTTGT GTGACCCCGT TTGCCTCAAT
    GGTGGTTCCT GTAATAAGCC AAACACTTGC CTCTGTCCAA ATGGATTCTT TGGTGCACAG
    TGTCAGAATG CTATCTGTCA CCCTCCCTGT AAGAATGGTG GCCGCTGCAT GAGAAATAAT
    GTGTGTGCCT GTCGTGAAGG ATATGCTGGG AAGAGATGCC AAAAAAGTAT CTGTGATCCC
    ATGTGCATGA ATGGAGGAAA ATGCGTGGGA CCAAACATTT GCTCTTGTCC TTCCGGTTGG
    AGAGGGAAAC AATGCAACAT AGCTCTCTGC TTTCAGAAAT GTAAAAATGG TGGCGAATGT
    ATTGCTCCCA GTATATGCCA TTGTCCTACC ACATGGGAAG GAGGGCAGTG TCAAATACCA
    ATTTGCAAAC AGAAGTGTCT TTATGGAGGG AGATGTGTAT TTCCCAATGT GTGCTCCTGT
    CGCAATGGAT ATTCTGGAGT CAAATGTGAA AAGAAAGTAC AGACAAAACG CCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293246
    Clone ID Related Accession (Same CDS sequence) XM_022427416.1
    Accession Version XM_022427416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5379bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGATCTGCC TATGTGGGTT TGACAGAAAA GTTCAGGAGT GCTCTCCTGG AGGACACCAG 
    CTTCTTCAGA GCCCTTATAG AAGTGTGCAT TTTGACTCAA TGCACCTCCA GCATTCTCCC
    TTTCAAGACC TAATATGTGA CCATTCCCTG TCTCCTGGAT GGTATCGATT TCTCATCTTT
    GACAGACCTG CCGAGATGCC CACCAAATGT GTTGAGATGA ATCACTGTGG AACACAGGCT
    CCTATCTGGC TGTCTCTGAG AGATACAGAG ACCCTGCCTT TACCTGGAGA GATCAAGCAA
    CTGACAGCTT GTGCTACATG GCAGGTTTTG TTTAGTGCTA CCAAAGACTG CTGCCTTTTT
    CAAATCCCAG TGTCTGTAAG AAACTGTGGA AATTTTTTTG TGTACTTACT ACAGCCCACT
    CAGGGATGTA TGGGATATTG TGCAGAAGCT ATTTCTGATG CAAAATTATA CCCATGTGAC
    CCTGATGAAA TTGAAATTGG AGGTAACTGT GTTCGTCAGC TGTCTGCTTC ATTGCCACCA
    CCACCCCCAG GAAGGCCGGA AGTTGTGGTG GAGCTGATTG GATCCAGGCT TTTCTGTAGG
    TGTGCTTTTG ATGTTTACCC TACAAACACC TCAGTGGGAT TTCTCATAAC TTGGTCTAGA
    CTTTCTTCTC AAGAAATCAA AGAGGAACTG AAGCAAGAGG CCACAGTTCA GGCATCCTCT
    CTATTAGAAC TGGATGGCAT AAATCTCAGA CTTGGAGATA GGATATTCTG TAGTGCTTCC
    ATCTTTTTAT TGGAGAAACC ACACGTACAA AGTATAGCCA TCGAAAGCCA AGAATTTTTT
    GCAGGCATTA AGTTACACCC TGAATTAAGC ACTATATCAG AAGATGGGAA AGAATATCAG
    TTGAAGATAG AGAGCACAGT TCCTGTCATT TGTTCTGAAT TCAATGAGCT TGACCAGGAA
    TGCAAAATCT CATTAAAACT GAAAACTGTT GAGCAAGGTA AAGAACAGCT AGGTTTAAAT
    TTGGCATTGT CTTCCTGTCA CGCGGACCTT CACCAAACAT CATCCTGTGC TAATGGATCC
    TGTAGCCATG CTTTTGTGTA CTACACTGCT GTAACAGATT TTTCTCGAGA TGGAGATCGA
    GTTACAAACA TTGTAGTGGA GCCTATAGTT CATGAGGATT TCCTGTGGAA CAGCTACATT
    CCAGACAGCA TCCAGATCAA AGTAAAGGAT GTCCCAACTG CTTACTGCTA TTCATTTACT
    GACCCACATA TAATTACATT TGATGGCAGG GTATATGATA ATTTCAAGAC TGGAACATTT
    GTGCTTTATA AGAGTATGTC ACGTGATTTT GAAGTCCATG TACGCCAGTG GGACTGTGGA
    AGCCTTCACT ATCCTGTGTC ATGTAACTGC GGGTTTGTTG CCAAAGAAGA AAGTGATATA
    GTAACTTTCG ACATGTGCAG TGGTCAGCTA CATGAATCAC AGCCATATTT ATCTGTAAAG
    AGTCAAGATG TAACCAGCAA TGTCAAGATA AGTGAATCTT ATTTAGGAAG AAAAGTCACA
    ATCTGGTTTT CTTCTGGAGC ATTTATCCGT GCTGATCTTA GTGAATGGGG CATGAGTTTA
    ACAATCAGAG CCCCTAGCCT AGACTACCAA AACACTCTGG GACTTTGTGG CACCTTTGAT
    AATAACCCAG AAAATGATTT CCATAATAAA AATGGGATAA AAATTGATCT AACATTTAAT
    AATTGTGTTG CTTTTATTAA TGAGTGGAGG ATTTTGCCGG GGAAAAGCAT GTTTGACACA
    ATGCCAATTT CTCCGCCATC ACCTGGAAAA CTCTCCTATT GTAGCTGCTC ATTGAACACT
    AATTCATTAT ATCCTTTTGA TCACCTGGAC AGTGTATCTC AACAAGAGAT TATTTCAGGT
    TGCAAAGATC TCAAACATGC CAAATTCTCT TTCCTGATAC CAGAACTAGA TGTCACCTCT
    GAATATATTA ATTCAGAAGC TCTCATCAGA GGAATAAATG AGTATACATC TGGAGAAGAA
    AATAACTTGA ACTTCCTGCC TCAAGAAAAA AAGCATGTGA ACCTAAGCAA ACTGGAATTA
    CATTTACAAA AACATCCTGG AAATGAAAAA CAAGAGGCAC CGAAGACTTT GGACAACGAG
    AAACATACAC AGGACCAGGG GAGCCACAGC CAGGAAATGA GGCGGGATCC ACAAAACAGA
    TGGAAACGGC AAAACTTTTA TGAGTTTCTT CCTATGTTTG CTTTCCAGAG TCTCAGCCAA
    AGTGACCTAG AAGAATTGAC TTATTTTTTC CCTGAGGACC ATACTGAGGA TGTCCACCAG
    GAGTTTGTCC CTACGTGGCC CACACCCTCT GGTCTCACCG AAGGCCACAC TTTGGCACTG
    TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA GGAAGACACT GTCTTGCCTT TCTGGGCAAG
    AGTCTGGACA ATGCTATAGA TATGTGTGTT AAGGATGTTC TCCTAAAAGA TGACCTCAGC
    TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA AATGAATGTG AAAAGAGAAT TTTGGAAGAG
    GGGAAATACA ATGCAGAAGA AAATAGCAAG TCAGTCGAAG ATATTCTCTT GGTACTAAAA
    TGCCCCAATT TATGCAGCGG CCATGGGCAG TGCATGGAAT GGGGATGCGC ATGTTCCCCT
    GGCTTTGGTT CCTATGACTG CAGTGATTCG CATGACATGC CTCCTGAAAT TATAGAACTT
    GAGAATGCTG GATTCTGTAA TATTCGAAAA TATAATTGTA TGATAGTGAG AGTGTTTGGT
    AAAGGCTTCA AAGAATCACC TTCAATTAAA TGTGAAGTTA CTAAACTGCA GTATAATAGC
    AGTAAATGGG TCCTTGGAAA AACTGTCTAC ACGCATACTG TTTTTCAAAA TAGCAGAACT
    GTTGATTGTC AGCTGCCCCC TGATGTTCAA CCATCTGATA CCATGAATCT GATGGATGAG
    AAACCAATTG CGAAGTGGCA ATTAAAGGTA TCCAATGATG GTTATAGGTT CAGTAATCCC
    AAAATAATGA TCATTTATGA TGGAGCTTGT CAAATTTGTG GTCTCTATGG GAACGACTCA
    TGTGCTCTAA AGGAAAATGT TTGCATTATT GATGGACTCT GCTACGTTGA AGAAGATAAA
    AATCCTACCA GCCCTTGTTT GATTTGTAAA CCAAAAATGT CAAAATTTAC TTGGTCAGTT
    TTAGAAAACA ACCAACCTCC AGTGATTCAA ATACCGCAAG ACAAATTACA GACATTTTTC
    GGGGAGAACT TTTTGTACCA GTTCACGGCC TTTGATCCTG AAGGTTCTGA CGTCCGTTTT
    ACCTTGGACT CGGGTCCTGA GGGAGCAAAT GTTTCCTCTT CAGGACTTCT TATGTGGAAA
    ACAGAATCCC AAACTCCACA GGAATTCACT CTACGTTTTA ATGACGACTG TAATGCTGAA
    ACGAGAGTCA CGATTATGGT GACTGTGAAG TCTTGTGATT GCTTGAATGG TGGATTATGC
    GTGTCAGACA CTAAATCTCC TCCAGGAAGT GGAATGTACC TGTGTGTCTG CTTGCCTGGC
    TTTCAGGGCA GTCTTTGTGA AGTGAACGTC ACTGAGTGCC AGTCAAACCC CTGTGGTCTT
    GGCAAATGTG TTAGTGGATT TCAGAGTTAT TCTTGTGTAT GTCCACCTGA ACTCAAAGGC
    AAAACTTGCC AGGAGGATGT TGATGAGTGT GAATCCAGGC CTTGCTTTCC AGGAGCAGAA
    TGTCTCAATA CCTTTGGTTC CTATCATTGT GGTGCATGTC CAAAAGGATT TTATGGTGAT
    GGGAAAATAT GTCATGATAC AGCATTTCAG CCACCATCAA CAGATCACTC ACCATCTGGG
    GCAAACTTTA CCCACAGTCC TCCTACTGTC AAGAAGTTCT TGGACACACC CAGTAACATC
    TCACACTTCT CATTAAAATC AGTTACAACT CAAATGAATA ATTCAAAGAA AGTTCTTTCC
    CATCAAATAT CAGGCTTTGA ACAGATGGAT CATACTATCA GTACAAATCT CAGTCTGGAG
    GAATTGGGAG TTTCCATGAA CAACCATAAT TCTTTAAGCG TTAACCCAAA TCATTCCCCT
    GGTGAAGTAA AGGATTTTAT ACAGAGCACC ATCCAAACAG AGGCAGGAGG GCATAATTCT
    CAAATTAACA GGTCTGCCCA AAGTTACACG CAGTATAAAG ATAGGTATGC CTCATCAGTT
    ACTAGAGCCA TCACTGTTTT GCCAGAAAAG CCTGGATGGA TGAAAATATC AACTTGTGCT
    GGATCACCTT GTTTTCTCGG TGTTTCTTGT GTGCCAACCA CAGATGGTCA CTTCCAATGT
    GGACGCTGCC CCTTTGGATA TTATGGAGAT GGTATCAATT GCAGAGGTAG TATGAAAATT
    CTTCAGAAGA TGGGAAGAAA ACTATTTGGT TCACATTTAA CCATTTGTAG ACACCCATGT
    GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC ATATGCAAAT GTAAACCTGG TTACACTGGT
    TCTAACTGCC AAACTGCTGT ATGTCATCCT GATTGCAAAA ACCATGGAAA ATGTATCAAG
    CCTAACATTT GTGACTGTCC CCCGGGACAT GGGGGTGCAA CCTGTGATGA AGAACATTGT
    AGCCCACCTT GTCAACATGG TGGCACCTGC TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC
    GGTTTTATAG GACCAAGGTG TGAAACAATG GTTTGTAACA GGCACTGTGA AAATGGAGGT
    GAATGTCTTA CACCAGATAT TTGCCAGTGC AAGCCTGGCT GGTATGGACC TACTTGTGGT
    ACAGCTTTGT GTGACCCCGT TTGCCTCAAT GGTGGTTCCT GTAATAAGCC AAACACTTGC
    CTCTGTCCAA ATGGATTCTT TGGTGCACAG TGTCAGAATG CTATCTGTCA CCCTCCCTGT
    AAGAATGGTG GCCGCTGCAT GAGAAATAAT GTGTGTGCCT GTCGTGAAGG ATATGCTGGG
    AAGAGATGCC AAAAAAGTAT CTGTGATCCC ATGTGCATGA ATGGAGGAAA ATGCGTGGGA
    CCAAACATTT GCTCTTGTCC TTCCGGTTGG AGAGGGAAAC AATGCAACAT AGCTCTCTGC
    TTTCAGAAAT GTAAAAATGG TGGCGAATGT ATTGCTCCCA GTATATGCCA TTGTCCTACC
    ACATGGGAAG GAGGGCAGTG TCAAATACCA ATTTGCAAAC AGAAGTGTCT TTATGGAGGG
    AGATGTGTAT TTCCCAATGT GTGCTCCTGT CGCAATGGAT ATTCTGGAGT CAAATGTGAA
    AAGAAAGTAC AGGATGCTGA GCCCAGGATG CATCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283124.1
    CDS281..5659
    Translation

    Target ORF information:

    RefSeq Version XM_022427416.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGATCTGCC TATGTGGGTT TGACAGAAAA GTTCAGGAGT GCTCTCCTGG AGGACACCAG 
    CTTCTTCAGA GCCCTTATAG AAGTGTGCAT TTTGACTCAA TGCACCTCCA GCATTCTCCC
    TTTCAAGACC TAATATGTGA CCATTCCCTG TCTCCTGGAT GGTATCGATT TCTCATCTTT
    GACAGACCTG CCGAGATGCC CACCAAATGT GTTGAGATGA ATCACTGTGG AACACAGGCT
    CCTATCTGGC TGTCTCTGAG AGATACAGAG ACCCTGCCTT TACCTGGAGA GATCAAGCAA
    CTGACAGCTT GTGCTACATG GCAGGTTTTG TTTAGTGCTA CCAAAGACTG CTGCCTTTTT
    CAAATCCCAG TGTCTGTAAG AAACTGTGGA AATTTTTTTG TGTACTTACT ACAGCCCACT
    CAGGGATGTA TGGGATATTG TGCAGAAGCT ATTTCTGATG CAAAATTATA CCCATGTGAC
    CCTGATGAAA TTGAAATTGG AGGTAACTGT GTTCGTCAGC TGTCTGCTTC ATTGCCACCA
    CCACCCCCAG GAAGGCCGGA AGTTGTGGTG GAGCTGATTG GATCCAGGCT TTTCTGTAGG
    TGTGCTTTTG ATGTTTACCC TACAAACACC TCAGTGGGAT TTCTCATAAC TTGGTCTAGA
    CTTTCTTCTC AAGAAATCAA AGAGGAACTG AAGCAAGAGG CCACAGTTCA GGCATCCTCT
    CTATTAGAAC TGGATGGCAT AAATCTCAGA CTTGGAGATA GGATATTCTG TAGTGCTTCC
    ATCTTTTTAT TGGAGAAACC ACACGTACAA AGTATAGCCA TCGAAAGCCA AGAATTTTTT
    GCAGGCATTA AGTTACACCC TGAATTAAGC ACTATATCAG AAGATGGGAA AGAATATCAG
    TTGAAGATAG AGAGCACAGT TCCTGTCATT TGTTCTGAAT TCAATGAGCT TGACCAGGAA
    TGCAAAATCT CATTAAAACT GAAAACTGTT GAGCAAGGTA AAGAACAGCT AGGTTTAAAT
    TTGGCATTGT CTTCCTGTCA CGCGGACCTT CACCAAACAT CATCCTGTGC TAATGGATCC
    TGTAGCCATG CTTTTGTGTA CTACACTGCT GTAACAGATT TTTCTCGAGA TGGAGATCGA
    GTTACAAACA TTGTAGTGGA GCCTATAGTT CATGAGGATT TCCTGTGGAA CAGCTACATT
    CCAGACAGCA TCCAGATCAA AGTAAAGGAT GTCCCAACTG CTTACTGCTA TTCATTTACT
    GACCCACATA TAATTACATT TGATGGCAGG GTATATGATA ATTTCAAGAC TGGAACATTT
    GTGCTTTATA AGAGTATGTC ACGTGATTTT GAAGTCCATG TACGCCAGTG GGACTGTGGA
    AGCCTTCACT ATCCTGTGTC ATGTAACTGC GGGTTTGTTG CCAAAGAAGA AAGTGATATA
    GTAACTTTCG ACATGTGCAG TGGTCAGCTA CATGAATCAC AGCCATATTT ATCTGTAAAG
    AGTCAAGATG TAACCAGCAA TGTCAAGATA AGTGAATCTT ATTTAGGAAG AAAAGTCACA
    ATCTGGTTTT CTTCTGGAGC ATTTATCCGT GCTGATCTTA GTGAATGGGG CATGAGTTTA
    ACAATCAGAG CCCCTAGCCT AGACTACCAA AACACTCTGG GACTTTGTGG CACCTTTGAT
    AATAACCCAG AAAATGATTT CCATAATAAA AATGGGATAA AAATTGATCT AACATTTAAT
    AATTGTGTTG CTTTTATTAA TGAGTGGAGG ATTTTGCCGG GGAAAAGCAT GTTTGACACA
    ATGCCAATTT CTCCGCCATC ACCTGGAAAA CTCTCCTATT GTAGCTGCTC ATTGAACACT
    AATTCATTAT ATCCTTTTGA TCACCTGGAC AGTGTATCTC AACAAGAGAT TATTTCAGGT
    TGCAAAGATC TCAAACATGC CAAATTCTCT TTCCTGATAC CAGAACTAGA TGTCACCTCT
    GAATATATTA ATTCAGAAGC TCTCATCAGA GGAATAAATG AGTATACATC TGGAGAAGAA
    AATAACTTGA ACTTCCTGCC TCAAGAAAAA AAGCATGTGA ACCTAAGCAA ACTGGAATTA
    CATTTACAAA AACATCCTGG AAATGAAAAA CAAGAGGCAC CGAAGACTTT GGACAACGAG
    AAACATACAC AGGACCAGGG GAGCCACAGC CAGGAAATGA GGCGGGATCC ACAAAACAGA
    TGGAAACGGC AAAACTTTTA TGAGTTTCTT CCTATGTTTG CTTTCCAGAG TCTCAGCCAA
    AGTGACCTAG AAGAATTGAC TTATTTTTTC CCTGAGGACC ATACTGAGGA TGTCCACCAG
    GAGTTTGTCC CTACGTGGCC CACACCCTCT GGTCTCACCG AAGGCCACAC TTTGGCACTG
    TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA GGAAGACACT GTCTTGCCTT TCTGGGCAAG
    AGTCTGGACA ATGCTATAGA TATGTGTGTT AAGGATGTTC TCCTAAAAGA TGACCTCAGC
    TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA AATGAATGTG AAAAGAGAAT TTTGGAAGAG
    GGGAAATACA ATGCAGAAGA AAATAGCAAG TCAGTCGAAG ATATTCTCTT GGTACTAAAA
    TGCCCCAATT TATGCAGCGG CCATGGGCAG TGCATGGAAT GGGGATGCGC ATGTTCCCCT
    GGCTTTGGTT CCTATGACTG CAGTGATTCG CATGACATGC CTCCTGAAAT TATAGAACTT
    GAGAATGCTG GATTCTGTAA TATTCGAAAA TATAATTGTA TGATAGTGAG AGTGTTTGGT
    AAAGGCTTCA AAGAATCACC TTCAATTAAA TGTGAAGTTA CTAAACTGCA GTATAATAGC
    AGTAAATGGG TCCTTGGAAA AACTGTCTAC ACGCATACTG TTTTTCAAAA TAGCAGAACT
    GTTGATTGTC AGCTGCCCCC TGATGTTCAA CCATCTGATA CCATGAATCT GATGGATGAG
    AAACCAATTG CGAAGTGGCA ATTAAAGGTA TCCAATGATG GTTATAGGTT CAGTAATCCC
    AAAATAATGA TCATTTATGA TGGAGCTTGT CAAATTTGTG GTCTCTATGG GAACGACTCA
    TGTGCTCTAA AGGAAAATGT TTGCATTATT GATGGACTCT GCTACGTTGA AGAAGATAAA
    AATCCTACCA GCCCTTGTTT GATTTGTAAA CCAAAAATGT CAAAATTTAC TTGGTCAGTT
    TTAGAAAACA ACCAACCTCC AGTGATTCAA ATACCGCAAG ACAAATTACA GACATTTTTC
    GGGGAGAACT TTTTGTACCA GTTCACGGCC TTTGATCCTG AAGGTTCTGA CGTCCGTTTT
    ACCTTGGACT CGGGTCCTGA GGGAGCAAAT GTTTCCTCTT CAGGACTTCT TATGTGGAAA
    ACAGAATCCC AAACTCCACA GGAATTCACT CTACGTTTTA ATGACGACTG TAATGCTGAA
    ACGAGAGTCA CGATTATGGT GACTGTGAAG TCTTGTGATT GCTTGAATGG TGGATTATGC
    GTGTCAGACA CTAAATCTCC TCCAGGAAGT GGAATGTACC TGTGTGTCTG CTTGCCTGGC
    TTTCAGGGCA GTCTTTGTGA AGTGAACGTC ACTGAGTGCC AGTCAAACCC CTGTGGTCTT
    GGCAAATGTG TTAGTGGATT TCAGAGTTAT TCTTGTGTAT GTCCACCTGA ACTCAAAGGC
    AAAACTTGCC AGGAGGATGT TGATGAGTGT GAATCCAGGC CTTGCTTTCC AGGAGCAGAA
    TGTCTCAATA CCTTTGGTTC CTATCATTGT GGTGCATGTC CAAAAGGATT TTATGGTGAT
    GGGAAAATAT GTCATGATAC AGCATTTCAG CCACCATCAA CAGATCACTC ACCATCTGGG
    GCAAACTTTA CCCACAGTCC TCCTACTGTC AAGAAGTTCT TGGACACACC CAGTAACATC
    TCACACTTCT CATTAAAATC AGTTACAACT CAAATGAATA ATTCAAAGAA AGTTCTTTCC
    CATCAAATAT CAGGCTTTGA ACAGATGGAT CATACTATCA GTACAAATCT CAGTCTGGAG
    GAATTGGGAG TTTCCATGAA CAACCATAAT TCTTTAAGCG TTAACCCAAA TCATTCCCCT
    GGTGAAGTAA AGGATTTTAT ACAGAGCACC ATCCAAACAG AGGCAGGAGG GCATAATTCT
    CAAATTAACA GGTCTGCCCA AAGTTACACG CAGTATAAAG ATAGGTATGC CTCATCAGTT
    ACTAGAGCCA TCACTGTTTT GCCAGAAAAG CCTGGATGGA TGAAAATATC AACTTGTGCT
    GGATCACCTT GTTTTCTCGG TGTTTCTTGT GTGCCAACCA CAGATGGTCA CTTCCAATGT
    GGACGCTGCC CCTTTGGATA TTATGGAGAT GGTATCAATT GCAGAGGTAG TATGAAAATT
    CTTCAGAAGA TGGGAAGAAA ACTATTTGGT TCACATTTAA CCATTTGTAG ACACCCATGT
    GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC ATATGCAAAT GTAAACCTGG TTACACTGGT
    TCTAACTGCC AAACTGCTGT ATGTCATCCT GATTGCAAAA ACCATGGAAA ATGTATCAAG
    CCTAACATTT GTGACTGTCC CCCGGGACAT GGGGGTGCAA CCTGTGATGA AGAACATTGT
    AGCCCACCTT GTCAACATGG TGGCACCTGC TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC
    GGTTTTATAG GACCAAGGTG TGAAACAATG GTTTGTAACA GGCACTGTGA AAATGGAGGT
    GAATGTCTTA CACCAGATAT TTGCCAGTGC AAGCCTGGCT GGTATGGACC TACTTGTGGT
    ACAGCTTTGT GTGACCCCGT TTGCCTCAAT GGTGGTTCCT GTAATAAGCC AAACACTTGC
    CTCTGTCCAA ATGGATTCTT TGGTGCACAG TGTCAGAATG CTATCTGTCA CCCTCCCTGT
    AAGAATGGTG GCCGCTGCAT GAGAAATAAT GTGTGTGCCT GTCGTGAAGG ATATGCTGGG
    AAGAGATGCC AAAAAAGTAT CTGTGATCCC ATGTGCATGA ATGGAGGAAA ATGCGTGGGA
    CCAAACATTT GCTCTTGTCC TTCCGGTTGG AGAGGGAAAC AATGCAACAT AGCTCTCTGC
    TTTCAGAAAT GTAAAAATGG TGGCGAATGT ATTGCTCCCA GTATATGCCA TTGTCCTACC
    ACATGGGAAG GAGGGCAGTG TCAAATACCA ATTTGCAAAC AGAAGTGTCT TTATGGAGGG
    AGATGTGTAT TTCCCAATGT GTGCTCCTGT CGCAATGGAT ATTCTGGAGT CAAATGTGAA
    AAGAAAGTAC AGGATGCTGA GCCCAGGATG CATCGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293247
    Clone ID Related Accession (Same CDS sequence) XM_022427417.1
    Accession Version XM_022427417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGGTAG TATGAAAATT CTTCAGAAGA TGGGAAGAAA ACTATTTGGT
    TCACATTTAA CCATTTGTAG ACACCCATGT GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC
    ATATGCAAAT GTAAACCTGG TTACACTGGT TCTAACTGCC AAACTGCTGT ATGTCATCCT
    GATTGCAAAA ACCATGGAAA ATGTATCAAG CCTAACATTT GTGACTGTCC CCCGGGACAT
    GGGGGTGCAA CCTGTGATGA AGAACATTGT AGCCCACCTT GTCAACATGG TGGCACCTGC
    TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC GGTTTTATAG GACCAAGGTG TGAAACAATG
    GTTTGTAACA GGCACTGTGA AAATGGAGGT GAATGTCTTA CACCAGATAT TTGCCAGTGC
    AAGCCTGGCT GGTATGGACC TACTTGTGGT ACAGCTTTGT GTGACCCCGT TTGCCTCAAT
    GGTGGTTCCT GTAATAAGCC AAACACTTGC CTCTGTCCAA ATGGATTCTT TGGTGCACAG
    TGTCAGAATG CTATCTGTCA CCCTCCCTGT AAGAATGGTG GCCGCTGCAT GAGAAATAAT
    GTGTGTGCCT GTCGTGAAGG ATATGCTGGG AAGAGATGCC AAAAAAGTAT CTGTGATCCC
    ATGTGCATGA ATGGAGGAAA ATGCGTGGGA CCAAACATTT GCTCTTGTCC TTCCGGTTGG
    AGAGGGAAAC AATGCAACAT AGCTCTCTGC TTTCAGAAAT GTAAAAATGG TGGCGAATGT
    ATTGCTCCCA GTATATGCCA TTGTCCTACC ACATGGGAAG GAGGGCAGTG TCAAATACAC
    AAAACGCCAT TAATATCAAG TTATGTGCAT CACAAGCCTG TTGTAAGAAT GAAATTTTTA
    ATTCAGAAAA AGTTGAAGAT AACTACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283125.1
    CDS184..5553
    Translation

    Target ORF information:

    RefSeq Version XM_022427417.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGGTAG TATGAAAATT CTTCAGAAGA TGGGAAGAAA ACTATTTGGT
    TCACATTTAA CCATTTGTAG ACACCCATGT GGAAAAAGCA GGGAGTGTGT AGCCCCAAAC
    ATATGCAAAT GTAAACCTGG TTACACTGGT TCTAACTGCC AAACTGCTGT ATGTCATCCT
    GATTGCAAAA ACCATGGAAA ATGTATCAAG CCTAACATTT GTGACTGTCC CCCGGGACAT
    GGGGGTGCAA CCTGTGATGA AGAACATTGT AGCCCACCTT GTCAACATGG TGGCACCTGC
    TTGGCTGGAA ATCTCTGCAC TTGTCCTTAC GGTTTTATAG GACCAAGGTG TGAAACAATG
    GTTTGTAACA GGCACTGTGA AAATGGAGGT GAATGTCTTA CACCAGATAT TTGCCAGTGC
    AAGCCTGGCT GGTATGGACC TACTTGTGGT ACAGCTTTGT GTGACCCCGT TTGCCTCAAT
    GGTGGTTCCT GTAATAAGCC AAACACTTGC CTCTGTCCAA ATGGATTCTT TGGTGCACAG
    TGTCAGAATG CTATCTGTCA CCCTCCCTGT AAGAATGGTG GCCGCTGCAT GAGAAATAAT
    GTGTGTGCCT GTCGTGAAGG ATATGCTGGG AAGAGATGCC AAAAAAGTAT CTGTGATCCC
    ATGTGCATGA ATGGAGGAAA ATGCGTGGGA CCAAACATTT GCTCTTGTCC TTCCGGTTGG
    AGAGGGAAAC AATGCAACAT AGCTCTCTGC TTTCAGAAAT GTAAAAATGG TGGCGAATGT
    ATTGCTCCCA GTATATGCCA TTGTCCTACC ACATGGGAAG GAGGGCAGTG TCAAATACAC
    AAAACGCCAT TAATATCAAG TTATGTGCAT CACAAGCCTG TTGTAAGAAT GAAATTTTTA
    ATTCAGAAAA AGTTGAAGAT AACTACTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293248
    Clone ID Related Accession (Same CDS sequence) XM_022427418.1
    Accession Version XM_022427418.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGCCAT TTGTAGACAC
    CCATGTGGAA AAAGCAGGGA GTGTGTAGCC CCAAACATAT GCAAATGTAA ACCTGGTTAC
    ACTGGTTCTA ACTGCCAAAC TGCTGTATGT CATCCTGATT GCAAAAACCA TGGAAAATGT
    ATCAAGCCTA ACATTTGTGA CTGTCCCCCG GGACATGGGG GTGCAACCTG TGATGAAGAA
    CATTGTAGCC CACCTTGTCA ACATGGTGGC ACCTGCTTGG CTGGAAATCT CTGCACTTGT
    CCTTACGGTT TTATAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGTGAAT GTCTTACACC AGATATTTGC CAGTGCAAGC CTGGCTGGTA TGGACCTACT
    TGTGGTACAG CTTTGTGTGA CCCCGTTTGC CTCAATGGTG GTTCCTGTAA TAAGCCAAAC
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGT GCACAGTGTC AGAATGCTAT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCG CTGCATGAGA AATAATGTGT GTGCCTGTCG TGAAGGATAT
    GCTGGGAAGA GATGCCAAAA AAGTATCTGT GATCCCATGT GCATGAATGG AGGAAAATGC
    GTGGGACCAA ACATTTGCTC TTGTCCTTCC GGTTGGAGAG GGAAACAATG CAACATAGCT
    CTCTGCTTTC AGAAATGTAA AAATGGTGGC GAATGTATTG CTCCCAGTAT ATGCCATTGT
    CCTACCACAT GGGAAGGAGG GCAGTGTCAA ATACCAATTT GCAAACAGAA GTGTCTTTAT
    GGAGGGAGAT GTGTATTTCC CAATGTGTGC TCCTGTCGCA ATGGATATTC TGGAGTCAAA
    TGTGAAAAGA AAGTACAGGA TGCTGAGCCC AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022283126.1
    CDS184..4968
    Translation

    Target ORF information:

    RefSeq Version XM_022427418.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022427418.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGCCAT TTGTAGACAC
    CCATGTGGAA AAAGCAGGGA GTGTGTAGCC CCAAACATAT GCAAATGTAA ACCTGGTTAC
    ACTGGTTCTA ACTGCCAAAC TGCTGTATGT CATCCTGATT GCAAAAACCA TGGAAAATGT
    ATCAAGCCTA ACATTTGTGA CTGTCCCCCG GGACATGGGG GTGCAACCTG TGATGAAGAA
    CATTGTAGCC CACCTTGTCA ACATGGTGGC ACCTGCTTGG CTGGAAATCT CTGCACTTGT
    CCTTACGGTT TTATAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGTGAAT GTCTTACACC AGATATTTGC CAGTGCAAGC CTGGCTGGTA TGGACCTACT
    TGTGGTACAG CTTTGTGTGA CCCCGTTTGC CTCAATGGTG GTTCCTGTAA TAAGCCAAAC
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGT GCACAGTGTC AGAATGCTAT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCG CTGCATGAGA AATAATGTGT GTGCCTGTCG TGAAGGATAT
    GCTGGGAAGA GATGCCAAAA AAGTATCTGT GATCCCATGT GCATGAATGG AGGAAAATGC
    GTGGGACCAA ACATTTGCTC TTGTCCTTCC GGTTGGAGAG GGAAACAATG CAACATAGCT
    CTCTGCTTTC AGAAATGTAA AAATGGTGGC GAATGTATTG CTCCCAGTAT ATGCCATTGT
    CCTACCACAT GGGAAGGAGG GCAGTGTCAA ATACCAATTT GCAAACAGAA GTGTCTTTAT
    GGAGGGAGAT GTGTATTTCC CAATGTGTGC TCCTGTCGCA ATGGATATTC TGGAGTCAAA
    TGTGAAAAGA AAGTACAGGA TGCTGAGCCC AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40041
    Clone ID Related Accession (Same CDS sequence) XM_014119190.1 , XM_014119190.2
    Accession Version XM_014119190.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGTATCTGT
    GATCCCATGT GCATGAATGG AGGAAAATGC GTGGGACCAA ACATTTGCTC TTGTCCTTCC
    GGTTGGAGAG GGAAACAATG CAACATAGCT CTCTGCTTTC AGAAATGTAA AAATGGTGGC
    GAATGTATTG CTCCCAGTAT ATGCCATTGT CCTACCACAT GGGAAGGAGG GCAGTGTCAA
    ATACCAATTT GCAAACAGAA GTGTCTTTAT GGAGGGAGAT GTGTATTTCC CAATGTGTGC
    TCCTGTCGCA ATGGATATTC TGGAGTCAAA TGTGAAAAGA AAGTACAGGA TGCTGAGCCC
    AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974665.1
    CDS180..5534
    Translation

    Target ORF information:

    RefSeq Version XM_014119190.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119190.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGTATCTGT
    GATCCCATGT GCATGAATGG AGGAAAATGC GTGGGACCAA ACATTTGCTC TTGTCCTTCC
    GGTTGGAGAG GGAAACAATG CAACATAGCT CTCTGCTTTC AGAAATGTAA AAATGGTGGC
    GAATGTATTG CTCCCAGTAT ATGCCATTGT CCTACCACAT GGGAAGGAGG GCAGTGTCAA
    ATACCAATTT GCAAACAGAA GTGTCTTTAT GGAGGGAGAT GTGTATTTCC CAATGTGTGC
    TCCTGTCGCA ATGGATATTC TGGAGTCAAA TGTGAAAAGA AAGTACAGGA TGCTGAGCCC
    AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40041
    Clone ID Related Accession (Same CDS sequence) XM_014119190.1 , XM_014119190.2
    Accession Version XM_014119190.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006596.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014119190.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGTATCTGT
    GATCCCATGT GCATGAATGG AGGAAAATGC GTGGGACCAA ACATTTGCTC TTGTCCTTCC
    GGTTGGAGAG GGAAACAATG CAACATAGCT CTCTGCTTTC AGAAATGTAA AAATGGTGGC
    GAATGTATTG CTCCCAGTAT ATGCCATTGT CCTACCACAT GGGAAGGAGG GCAGTGTCAA
    ATACCAATTT GCAAACAGAA GTGTCTTTAT GGAGGGAGAT GTGTATTTCC CAATGTGTGC
    TCCTGTCGCA ATGGATATTC TGGAGTCAAA TGTGAAAAGA AAGTACAGGA TGCTGAGCCC
    AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974665.1
    CDS184..5538
    Translation

    Target ORF information:

    RefSeq Version XM_014119190.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119190.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGTATCTGT
    GATCCCATGT GCATGAATGG AGGAAAATGC GTGGGACCAA ACATTTGCTC TTGTCCTTCC
    GGTTGGAGAG GGAAACAATG CAACATAGCT CTCTGCTTTC AGAAATGTAA AAATGGTGGC
    GAATGTATTG CTCCCAGTAT ATGCCATTGT CCTACCACAT GGGAAGGAGG GCAGTGTCAA
    ATACCAATTT GCAAACAGAA GTGTCTTTAT GGAGGGAGAT GTGTATTTCC CAATGTGTGC
    TCCTGTCGCA ATGGATATTC TGGAGTCAAA TGTGAAAAGA AAGTACAGGA TGCTGAGCCC
    AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40042
    Clone ID Related Accession (Same CDS sequence) XM_014119191.1
    Accession Version XM_014119191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGTATCTGT
    GATCCCATGT GCATGAATGG AGGAAAATGC GTGGGACCAA ACATTTGCTC TTGTCCTTCC
    GGTTGGAGAG GGAAACAATG CAACATAGCT CTCTGCTTTC AGAAATGTAA AAATGGTGGC
    GAATGTATTG CTCCCAGTAT ATGCCATTGT CCTACCACAT GGGAAGGAGG GCAGTGTCAA
    ATACCAATTT GCAAACAGAA GTGTCTTTAT GGAGGGAGAT GTGTATTTCC CAATGTGTGC
    TCCTGTCGCA ATGGATATTC TGGAGTCAAA TGTGAAAAGA AAGTACAGAC AAAACGCCAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974666.1
    CDS180..5522
    Translation

    Target ORF information:

    RefSeq Version XM_014119191.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGTATCTGT
    GATCCCATGT GCATGAATGG AGGAAAATGC GTGGGACCAA ACATTTGCTC TTGTCCTTCC
    GGTTGGAGAG GGAAACAATG CAACATAGCT CTCTGCTTTC AGAAATGTAA AAATGGTGGC
    GAATGTATTG CTCCCAGTAT ATGCCATTGT CCTACCACAT GGGAAGGAGG GCAGTGTCAA
    ATACCAATTT GCAAACAGAA GTGTCTTTAT GGAGGGAGAT GTGTATTTCC CAATGTGTGC
    TCCTGTCGCA ATGGATATTC TGGAGTCAAA TGTGAAAAGA AAGTACAGAC AAAACGCCAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40043
    Clone ID Related Accession (Same CDS sequence) XM_014119192.1
    Accession Version XM_014119192.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGATCTGCC TATGTGGGTT TGACAGAAAA GTTCAGGAGT GCTCTCCTGG AGGACACCAG 
    CTTCTTCAGA GCCCTTATAG AAGTGTGCAT TTTGACTCAA TGCACCTCCA GCATTCTCCC
    TTTCAAGACC TAATATGTGA CCATTCCCTG TCTCCTGGAT GGTATCGATT TCTCATCTTT
    GACAGACCTG CCGAGATGCC CACCAAATGT GTTGAGATGA ATCACTGTGG AACACAGGCT
    CCTATCTGGC TGTCTCTGAG AGATACAGAG ACCCTGCCTT TACCTGGAGA GATCAAGCAA
    CTGACAGCTT GTGCTACATG GCAGGTTTTG TTTAGTGCTA CCAAAGACTG CTGCCTTTTT
    CAAATCCCAG TGTCTGTAAG AAACTGTGGA AATTTTTTTG TGTACTTACT ACAGCCCACT
    CAGGGATGTA TGGGATATTG TGCAGAAGCT ATTTCTGATG CAAAATTATA CCCATGTGAC
    CCTGATGAAA TTGAAATTGG AGGTAACTGT GTTCGTCAGC TGTCTGCTTC ATTGCCACCA
    CCACCCCCAG GAAGGCCGGA AGTTGTGGTG GAGCTGATTG GATCCAGGCT TTTCTGTAGG
    TGTGCTTTTG ATGTTTACCC TACAAACACC TCAGTGGGAT TTCTCATAAC TTGGTCTAGA
    CTTTCTTCTC AAGAAATCAA AGAGGAACTG AAGCAAGAGG CCACAGTTCA GGCATCCTCT
    CTATTAGAAC TGGATGGCAT AAATCTCAGA CTTGGAGATA GGATATTCTG TAGTGCTTCC
    ATCTTTTTAT TGGAGAAACC ACACGTACAA AGTATAGCCA TCGAAAGCCA AGAATTTTTT
    GCAGGCATTA AGTTACACCC TGAATTAAGC ACTATATCAG AAGATGGGAA AGAATATCAG
    TTGAAGATAG AGAGCACAGT TCCTGTCATT TGTTCTGAAT TCAATGAGCT TGACCAGGAA
    TGCAAAATCT CATTAAAACT GAAAACTGTT GAGCAAGGTA AAGAACAGCT AGGTTTAAAT
    TTGGCATTGT CTTCCTGTCA CGCGGACCTT CACCAAACAT CATCCTGTGC TAATGGATCC
    TGTAGCCATG CTTTTGTGTA CTACACTGCT GTAACAGATT TTTCTCGAGA TGGAGATCGA
    GTTACAAACA TTGTAGTGGA GCCTATAGTT CATGAGGATT TCCTGTGGAA CAGCTACATT
    CCAGACAGCA TCCAGATCAA AGTAAAGGAT GTCCCAACTG CTTACTGCTA TTCATTTACT
    GACCCACATA TAATTACATT TGATGGCAGG GTATATGATA ATTTCAAGAC TGGAACATTT
    GTGCTTTATA AGAGTATGTC ACGTGATTTT GAAGTCCATG TACGCCAGTG GGACTGTGGA
    AGCCTTCACT ATCCTGTGTC ATGTAACTGC GGGTTTGTTG CCAAAGAAGA AAGTGATATA
    GTAACTTTCG ACATGTGCAG TGGTCAGCTA CATGAATCAC AGCCATATTT ATCTGTAAAG
    AGTCAAGATG TAACCAGCAA TGTCAAGATA AGTGAATCTT ATTTAGGAAG AAAAGTCACA
    ATCTGGTTTT CTTCTGGAGC ATTTATCCGT GCTGATCTTA GTGAATGGGG CATGAGTTTA
    ACAATCAGAG CCCCTAGCCT AGACTACCAA AACACTCTGG GACTTTGTGG CACCTTTGAT
    AATAACCCAG AAAATGATTT CCATAATAAA AATGGGATAA AAATTGATCT AACATTTAAT
    AATTGTGTTG CTTTTATTAA TGAGTGGAGG ATTTTGCCGG GGAAAAGCAT GTTTGACACA
    ATGCCAATTT CTCCGCCATC ACCTGGAAAA CTCTCCTATT GTAGCTGCTC ATTGAACACT
    AATTCATTAT ATCCTTTTGA TCACCTGGAC AGTGTATCTC AACAAGAGAT TATTTCAGGT
    TGCAAAGATC TCAAACATGC CAAATTCTCT TTCCTGATAC CAGAACTAGA TGTCACCTCT
    GAATATATTA ATTCAGAAGC TCTCATCAGA GGAATAAATG AGTATACATC TGGAGAAGAA
    AATAACTTGA ACTTCCTGCC TCAAGAAAAA AAGCATGTGA ACCTAAGCAA ACTGGAATTA
    CATTTACAAA AACATCCTGG AAATGAAAAA CAAGAGGCAC CGAAGACTTT GGACAACGAG
    AAACATACAC AGGACCAGGG GAGCCACAGC CAGGAAATGA GGCGGGATCC ACAAAACAGA
    TGGAAACGGC AAAACTTTTA TGAGTTTCTT CCTATGTTTG CTTTCCAGAG TCTCAGCCAA
    AGTGACCTAG AAGAATTGAC TTATTTTTTC CCTGAGGACC ATACTGAGGA TGTCCACCAG
    GAGTTTGTCC CTACGTGGCC CACACCCTCT GGTCTCACCG AAGGCCACAC TTTGGCACTG
    TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA GGAAGACACT GTCTTGCCTT TCTGGGCAAG
    AGTCTGGACA ATGCTATAGA TATGTGTGTT AAGGATGTTC TCCTAAAAGA TGACCTCAGC
    TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA AATGAATGTG AAAAGAGAAT TTTGGAAGAG
    GGGAAATACA ATGCAGAAGA AAATAGCAAG TCAGTCGAAG ATATTCTCTT GGTACTAAAA
    TGCCCCAATT TATGCAGCGG CCATGGGCAG TGCATGGAAT GGGGATGCGC ATGTTCCCCT
    GGCTTTGGTT CCTATGACTG CAGTGATTCG CATGACATGC CTCCTGAAAT TATAGAACTT
    GAGAATGCTG GATTCTGTAA TATTCGAAAA TATAATTGTA TGATAGTGAG AGTGTTTGGT
    AAAGGCTTCA AAGAATCACC TTCAATTAAA TGTGAAGTTA CTAAACTGCA GTATAATAGC
    AGTAAATGGG TCCTTGGAAA AACTGTCTAC ACGCATACTG TTTTTCAAAA TAGCAGAACT
    GTTGATTGTC AGCTGCCCCC TGATGTTCAA CCATCTGATA CCATGAATCT GATGGATGAG
    AAACCAATTG CGAAGTGGCA ATTAAAGGTA TCCAATGATG GTTATAGGTT CAGTAATCCC
    AAAATAATGA TCATTTATGA TGGAGCTTGT CAAATTTGTG GTCTCTATGG GAACGACTCA
    TGTGCTCTAA AGGAAAATGT TTGCATTATT GATGGACTCT GCTACGTTGA AGAAGATAAA
    AATCCTACCA GCCCTTGTTT GATTTGTAAA CCAAAAATGT CAAAATTTAC TTGGTCAGTT
    TTAGAAAACA ACCAACCTCC AGTGATTCAA ATACCGCAAG ACAAATTACA GACATTTTTC
    GGGGAGAACT TTTTGTACCA GTTCACGGCC TTTGATCCTG AAGGTTCTGA CGTCCGTTTT
    ACCTTGGACT CGGGTCCTGA GGGAGCAAAT GTTTCCTCTT CAGGACTTCT TATGTGGAAA
    ACAGAATCCC AAACTCCACA GGAATTCACT CTACGTTTTA ATGACGACTG TAATGCTGAA
    ACGAGAGTCA CGATTATGGT GACTGTGAAG TCTTGTGATT GCTTGAATGG TGGATTATGC
    GTGTCAGACA CTAAATCTCC TCCAGGAAGT GGAATGTACC TGTGTGTCTG CTTGCCTGGC
    TTTCAGGGCA GTCTTTGTGA AGTGAACGTC ACTGAGTGCC AGTCAAACCC CTGTGGTCTT
    GGCAAATGTG TTAGTGGATT TCAGAGTTAT TCTTGTGTAT GTCCACCTGA ACTCAAAGGC
    AAAACTTGCC AGGAGGATGT TGATGAGTGT GAATCCAGGC CTTGCTTTCC AGGAGCAGAA
    TGTCTCAATA CCTTTGGTTC CTATCATTGT GGTGCATGTC CAAAAGGATT TTATGGTGAT
    GGGAAAATAT GTCATGATAC AGCATTTCAG CCACCATCAA CAGATCACTC ACCATCTGGG
    GCAAACTTTA CCCACAGTCC TCCTACTGTC AAGAAGTTCT TGGACACACC CAGTAACATC
    TCACACTTCT CATTAAAATC AGTTACAACT CAAATGAATA ATTCAAAGAA AGTTCTTTCC
    CATCAAATAT CAGGCTTTGA ACAGATGGAT CATACTATCA GTACAAATCT CAGTCTGGAG
    GAATTGGGAG TTTCCATGAA CAACCATAAT TCTTTAAGCG TTAACCCAAA TCATTCCCCT
    GGTGAAGTAA AGGATTTTAT ACAGAGCACC ATCCAAACAG AGGCAGGAGG GCATAATTCT
    CAAATTAACA GGTCTGCCCA AAGTTACACG CAGTATAAAG ATAGGTATGC CTCATCAGTT
    ACTAGAGCCA TCACTGTTTT GCCAGAAAAG CCTGGATGGA TGAAAATATC AACTTGTGCT
    GGATCACCTT GTTTTCTCGG TGTTTCTTGT GTGCCAACCA CAGATGGTCA CTTCCAATGT
    GGACGCTGCC CCTTTGGATA TTATGGAGAT GGTATCAATT GCAGAGCCAT TTGTAGACAC
    CCATGTGGAA AAAGCAGGGA GTGTGTAGCC CCAAACATAT GCAAATGTAA ACCTGGTTAC
    ACTGGTTCTA ACTGCCAAAC TGCTGTATGT CATCCTGATT GCAAAAACCA TGGAAAATGT
    ATCAAGCCTA ACATTTGTGA CTGTCCCCCG GGACATGGGG GTGCAACCTG TGATGAAGAA
    CATTGTAGCC CACCTTGTCA ACATGGTGGC ACCTGCTTGG CTGGAAATCT CTGCACTTGT
    CCTTACGGTT TTATAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGTGAAT GTCTTACACC AGATATTTGC CAGTGCAAGC CTGGCTGGTA TGGACCTACT
    TGTGGTACAG CTTTGTGTGA CCCCGTTTGC CTCAATGGTG GTTCCTGTAA TAAGCCAAAC
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGT GCACAGTGTC AGAATGCTAT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCG CTGCATGAGA AATAATGTGT GTGCCTGTCG TGAAGGATAT
    GCTGGGAAGA GATGCCAAAA AAGTATCTGT GATCCCATGT GCATGAATGG AGGAAAATGC
    GTGGGACCAA ACATTTGCTC TTGTCCTTCC GGTTGGAGAG GGAAACAATG CAACATAGCT
    CTCTGCTTTC AGAAATGTAA AAATGGTGGC GAATGTATTG CTCCCAGTAT ATGCCATTGT
    CCTACCACAT GGGAAGGAGG GCAGTGTCAA ATACCAATTT GCAAACAGAA GTGTCTTTAT
    GGAGGGAGAT GTGTATTTCC CAATGTGTGC TCCTGTCGCA ATGGATATTC TGGAGTCAAA
    TGTGAAAAGA AAGTACAGGA TGCTGAGCCC AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974667.1
    CDS106..5430
    Translation

    Target ORF information:

    RefSeq Version XM_014119192.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGATCTGCC TATGTGGGTT TGACAGAAAA GTTCAGGAGT GCTCTCCTGG AGGACACCAG 
    CTTCTTCAGA GCCCTTATAG AAGTGTGCAT TTTGACTCAA TGCACCTCCA GCATTCTCCC
    TTTCAAGACC TAATATGTGA CCATTCCCTG TCTCCTGGAT GGTATCGATT TCTCATCTTT
    GACAGACCTG CCGAGATGCC CACCAAATGT GTTGAGATGA ATCACTGTGG AACACAGGCT
    CCTATCTGGC TGTCTCTGAG AGATACAGAG ACCCTGCCTT TACCTGGAGA GATCAAGCAA
    CTGACAGCTT GTGCTACATG GCAGGTTTTG TTTAGTGCTA CCAAAGACTG CTGCCTTTTT
    CAAATCCCAG TGTCTGTAAG AAACTGTGGA AATTTTTTTG TGTACTTACT ACAGCCCACT
    CAGGGATGTA TGGGATATTG TGCAGAAGCT ATTTCTGATG CAAAATTATA CCCATGTGAC
    CCTGATGAAA TTGAAATTGG AGGTAACTGT GTTCGTCAGC TGTCTGCTTC ATTGCCACCA
    CCACCCCCAG GAAGGCCGGA AGTTGTGGTG GAGCTGATTG GATCCAGGCT TTTCTGTAGG
    TGTGCTTTTG ATGTTTACCC TACAAACACC TCAGTGGGAT TTCTCATAAC TTGGTCTAGA
    CTTTCTTCTC AAGAAATCAA AGAGGAACTG AAGCAAGAGG CCACAGTTCA GGCATCCTCT
    CTATTAGAAC TGGATGGCAT AAATCTCAGA CTTGGAGATA GGATATTCTG TAGTGCTTCC
    ATCTTTTTAT TGGAGAAACC ACACGTACAA AGTATAGCCA TCGAAAGCCA AGAATTTTTT
    GCAGGCATTA AGTTACACCC TGAATTAAGC ACTATATCAG AAGATGGGAA AGAATATCAG
    TTGAAGATAG AGAGCACAGT TCCTGTCATT TGTTCTGAAT TCAATGAGCT TGACCAGGAA
    TGCAAAATCT CATTAAAACT GAAAACTGTT GAGCAAGGTA AAGAACAGCT AGGTTTAAAT
    TTGGCATTGT CTTCCTGTCA CGCGGACCTT CACCAAACAT CATCCTGTGC TAATGGATCC
    TGTAGCCATG CTTTTGTGTA CTACACTGCT GTAACAGATT TTTCTCGAGA TGGAGATCGA
    GTTACAAACA TTGTAGTGGA GCCTATAGTT CATGAGGATT TCCTGTGGAA CAGCTACATT
    CCAGACAGCA TCCAGATCAA AGTAAAGGAT GTCCCAACTG CTTACTGCTA TTCATTTACT
    GACCCACATA TAATTACATT TGATGGCAGG GTATATGATA ATTTCAAGAC TGGAACATTT
    GTGCTTTATA AGAGTATGTC ACGTGATTTT GAAGTCCATG TACGCCAGTG GGACTGTGGA
    AGCCTTCACT ATCCTGTGTC ATGTAACTGC GGGTTTGTTG CCAAAGAAGA AAGTGATATA
    GTAACTTTCG ACATGTGCAG TGGTCAGCTA CATGAATCAC AGCCATATTT ATCTGTAAAG
    AGTCAAGATG TAACCAGCAA TGTCAAGATA AGTGAATCTT ATTTAGGAAG AAAAGTCACA
    ATCTGGTTTT CTTCTGGAGC ATTTATCCGT GCTGATCTTA GTGAATGGGG CATGAGTTTA
    ACAATCAGAG CCCCTAGCCT AGACTACCAA AACACTCTGG GACTTTGTGG CACCTTTGAT
    AATAACCCAG AAAATGATTT CCATAATAAA AATGGGATAA AAATTGATCT AACATTTAAT
    AATTGTGTTG CTTTTATTAA TGAGTGGAGG ATTTTGCCGG GGAAAAGCAT GTTTGACACA
    ATGCCAATTT CTCCGCCATC ACCTGGAAAA CTCTCCTATT GTAGCTGCTC ATTGAACACT
    AATTCATTAT ATCCTTTTGA TCACCTGGAC AGTGTATCTC AACAAGAGAT TATTTCAGGT
    TGCAAAGATC TCAAACATGC CAAATTCTCT TTCCTGATAC CAGAACTAGA TGTCACCTCT
    GAATATATTA ATTCAGAAGC TCTCATCAGA GGAATAAATG AGTATACATC TGGAGAAGAA
    AATAACTTGA ACTTCCTGCC TCAAGAAAAA AAGCATGTGA ACCTAAGCAA ACTGGAATTA
    CATTTACAAA AACATCCTGG AAATGAAAAA CAAGAGGCAC CGAAGACTTT GGACAACGAG
    AAACATACAC AGGACCAGGG GAGCCACAGC CAGGAAATGA GGCGGGATCC ACAAAACAGA
    TGGAAACGGC AAAACTTTTA TGAGTTTCTT CCTATGTTTG CTTTCCAGAG TCTCAGCCAA
    AGTGACCTAG AAGAATTGAC TTATTTTTTC CCTGAGGACC ATACTGAGGA TGTCCACCAG
    GAGTTTGTCC CTACGTGGCC CACACCCTCT GGTCTCACCG AAGGCCACAC TTTGGCACTG
    TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA GGAAGACACT GTCTTGCCTT TCTGGGCAAG
    AGTCTGGACA ATGCTATAGA TATGTGTGTT AAGGATGTTC TCCTAAAAGA TGACCTCAGC
    TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA AATGAATGTG AAAAGAGAAT TTTGGAAGAG
    GGGAAATACA ATGCAGAAGA AAATAGCAAG TCAGTCGAAG ATATTCTCTT GGTACTAAAA
    TGCCCCAATT TATGCAGCGG CCATGGGCAG TGCATGGAAT GGGGATGCGC ATGTTCCCCT
    GGCTTTGGTT CCTATGACTG CAGTGATTCG CATGACATGC CTCCTGAAAT TATAGAACTT
    GAGAATGCTG GATTCTGTAA TATTCGAAAA TATAATTGTA TGATAGTGAG AGTGTTTGGT
    AAAGGCTTCA AAGAATCACC TTCAATTAAA TGTGAAGTTA CTAAACTGCA GTATAATAGC
    AGTAAATGGG TCCTTGGAAA AACTGTCTAC ACGCATACTG TTTTTCAAAA TAGCAGAACT
    GTTGATTGTC AGCTGCCCCC TGATGTTCAA CCATCTGATA CCATGAATCT GATGGATGAG
    AAACCAATTG CGAAGTGGCA ATTAAAGGTA TCCAATGATG GTTATAGGTT CAGTAATCCC
    AAAATAATGA TCATTTATGA TGGAGCTTGT CAAATTTGTG GTCTCTATGG GAACGACTCA
    TGTGCTCTAA AGGAAAATGT TTGCATTATT GATGGACTCT GCTACGTTGA AGAAGATAAA
    AATCCTACCA GCCCTTGTTT GATTTGTAAA CCAAAAATGT CAAAATTTAC TTGGTCAGTT
    TTAGAAAACA ACCAACCTCC AGTGATTCAA ATACCGCAAG ACAAATTACA GACATTTTTC
    GGGGAGAACT TTTTGTACCA GTTCACGGCC TTTGATCCTG AAGGTTCTGA CGTCCGTTTT
    ACCTTGGACT CGGGTCCTGA GGGAGCAAAT GTTTCCTCTT CAGGACTTCT TATGTGGAAA
    ACAGAATCCC AAACTCCACA GGAATTCACT CTACGTTTTA ATGACGACTG TAATGCTGAA
    ACGAGAGTCA CGATTATGGT GACTGTGAAG TCTTGTGATT GCTTGAATGG TGGATTATGC
    GTGTCAGACA CTAAATCTCC TCCAGGAAGT GGAATGTACC TGTGTGTCTG CTTGCCTGGC
    TTTCAGGGCA GTCTTTGTGA AGTGAACGTC ACTGAGTGCC AGTCAAACCC CTGTGGTCTT
    GGCAAATGTG TTAGTGGATT TCAGAGTTAT TCTTGTGTAT GTCCACCTGA ACTCAAAGGC
    AAAACTTGCC AGGAGGATGT TGATGAGTGT GAATCCAGGC CTTGCTTTCC AGGAGCAGAA
    TGTCTCAATA CCTTTGGTTC CTATCATTGT GGTGCATGTC CAAAAGGATT TTATGGTGAT
    GGGAAAATAT GTCATGATAC AGCATTTCAG CCACCATCAA CAGATCACTC ACCATCTGGG
    GCAAACTTTA CCCACAGTCC TCCTACTGTC AAGAAGTTCT TGGACACACC CAGTAACATC
    TCACACTTCT CATTAAAATC AGTTACAACT CAAATGAATA ATTCAAAGAA AGTTCTTTCC
    CATCAAATAT CAGGCTTTGA ACAGATGGAT CATACTATCA GTACAAATCT CAGTCTGGAG
    GAATTGGGAG TTTCCATGAA CAACCATAAT TCTTTAAGCG TTAACCCAAA TCATTCCCCT
    GGTGAAGTAA AGGATTTTAT ACAGAGCACC ATCCAAACAG AGGCAGGAGG GCATAATTCT
    CAAATTAACA GGTCTGCCCA AAGTTACACG CAGTATAAAG ATAGGTATGC CTCATCAGTT
    ACTAGAGCCA TCACTGTTTT GCCAGAAAAG CCTGGATGGA TGAAAATATC AACTTGTGCT
    GGATCACCTT GTTTTCTCGG TGTTTCTTGT GTGCCAACCA CAGATGGTCA CTTCCAATGT
    GGACGCTGCC CCTTTGGATA TTATGGAGAT GGTATCAATT GCAGAGCCAT TTGTAGACAC
    CCATGTGGAA AAAGCAGGGA GTGTGTAGCC CCAAACATAT GCAAATGTAA ACCTGGTTAC
    ACTGGTTCTA ACTGCCAAAC TGCTGTATGT CATCCTGATT GCAAAAACCA TGGAAAATGT
    ATCAAGCCTA ACATTTGTGA CTGTCCCCCG GGACATGGGG GTGCAACCTG TGATGAAGAA
    CATTGTAGCC CACCTTGTCA ACATGGTGGC ACCTGCTTGG CTGGAAATCT CTGCACTTGT
    CCTTACGGTT TTATAGGACC AAGGTGTGAA ACAATGGTTT GTAACAGGCA CTGTGAAAAT
    GGAGGTGAAT GTCTTACACC AGATATTTGC CAGTGCAAGC CTGGCTGGTA TGGACCTACT
    TGTGGTACAG CTTTGTGTGA CCCCGTTTGC CTCAATGGTG GTTCCTGTAA TAAGCCAAAC
    ACTTGCCTCT GTCCAAATGG ATTCTTTGGT GCACAGTGTC AGAATGCTAT CTGTCACCCT
    CCCTGTAAGA ATGGTGGCCG CTGCATGAGA AATAATGTGT GTGCCTGTCG TGAAGGATAT
    GCTGGGAAGA GATGCCAAAA AAGTATCTGT GATCCCATGT GCATGAATGG AGGAAAATGC
    GTGGGACCAA ACATTTGCTC TTGTCCTTCC GGTTGGAGAG GGAAACAATG CAACATAGCT
    CTCTGCTTTC AGAAATGTAA AAATGGTGGC GAATGTATTG CTCCCAGTAT ATGCCATTGT
    CCTACCACAT GGGAAGGAGG GCAGTGTCAA ATACCAATTT GCAAACAGAA GTGTCTTTAT
    GGAGGGAGAT GTGTATTTCC CAATGTGTGC TCCTGTCGCA ATGGATATTC TGGAGTCAAA
    TGTGAAAAGA AAGTACAGGA TGCTGAGCCC AGGATGCATC GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40044
    Clone ID Related Accession (Same CDS sequence) XM_014119193.1
    Accession Version XM_014119193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product von Willebrand factor D and EGF domain-containing protein isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726084.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGGCTGTTT
    CATCCTTTTA CCTTAAGGTA TCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013974668.1
    CDS180..5246
    Translation

    Target ORF information:

    RefSeq Version XM_014119193.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris von Willebrand factor D and EGF domains (VWDE), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTCGGCC GGGTCTGGGC GCTGGCCCTG CTGCTCCTGG CCCCGGGCCG AGGTCGGCAA 
    GTTCAGGAGT GCTCTCCTGG AGGACACCAG CTTCTTCAGA GCCCTTATAG AAGTGTGCAT
    TTTGACTCAA TGCACCTCCA GCATTCTCCC TTTCAAGACC TAATATGTGA CCATTCCCTG
    TCTCCTGGAT GGTATCGATT TCTCATCTTT GACAGACCTG CCGAGATGCC CACCAAATGT
    GTTGAGATGA ATCACTGTGG AACACAGGCT CCTATCTGGC TGTCTCTGAG AGATACAGAG
    ACCCTGCCTT TACCTGGAGA GATCAAGCAA CTGACAGCTT GTGCTACATG GCAGGTTTTG
    TTTAGTGCTA CCAAAGACTG CTGCCTTTTT CAAATCCCAG TGTCTGTAAG AAACTGTGGA
    AATTTTTTTG TGTACTTACT ACAGCCCACT CAGGGATGTA TGGGATATTG TGCAGAAGCT
    ATTTCTGATG CAAAATTATA CCCATGTGAC CCTGATGAAA TTGAAATTGG AGGTAACTGT
    GTTCGTCAGC TGTCTGCTTC ATTGCCACCA CCACCCCCAG GAAGGCCGGA AGTTGTGGTG
    GAGCTGATTG GATCCAGGCT TTTCTGTAGG TGTGCTTTTG ATGTTTACCC TACAAACACC
    TCAGTGGGAT TTCTCATAAC TTGGTCTAGA CTTTCTTCTC AAGAAATCAA AGAGGAACTG
    AAGCAAGAGG CCACAGTTCA GGCATCCTCT CTATTAGAAC TGGATGGCAT AAATCTCAGA
    CTTGGAGATA GGATATTCTG TAGTGCTTCC ATCTTTTTAT TGGAGAAACC ACACGTACAA
    AGTATAGCCA TCGAAAGCCA AGAATTTTTT GCAGGCATTA AGTTACACCC TGAATTAAGC
    ACTATATCAG AAGATGGGAA AGAATATCAG TTGAAGATAG AGAGCACAGT TCCTGTCATT
    TGTTCTGAAT TCAATGAGCT TGACCAGGAA TGCAAAATCT CATTAAAACT GAAAACTGTT
    GAGCAAGGTA AAGAACAGCT AGGTTTAAAT TTGGCATTGT CTTCCTGTCA CGCGGACCTT
    CACCAAACAT CATCCTGTGC TAATGGATCC TGTAGCCATG CTTTTGTGTA CTACACTGCT
    GTAACAGATT TTTCTCGAGA TGGAGATCGA GTTACAAACA TTGTAGTGGA GCCTATAGTT
    CATGAGGATT TCCTGTGGAA CAGCTACATT CCAGACAGCA TCCAGATCAA AGTAAAGGAT
    GTCCCAACTG CTTACTGCTA TTCATTTACT GACCCACATA TAATTACATT TGATGGCAGG
    GTATATGATA ATTTCAAGAC TGGAACATTT GTGCTTTATA AGAGTATGTC ACGTGATTTT
    GAAGTCCATG TACGCCAGTG GGACTGTGGA AGCCTTCACT ATCCTGTGTC ATGTAACTGC
    GGGTTTGTTG CCAAAGAAGA AAGTGATATA GTAACTTTCG ACATGTGCAG TGGTCAGCTA
    CATGAATCAC AGCCATATTT ATCTGTAAAG AGTCAAGATG TAACCAGCAA TGTCAAGATA
    AGTGAATCTT ATTTAGGAAG AAAAGTCACA ATCTGGTTTT CTTCTGGAGC ATTTATCCGT
    GCTGATCTTA GTGAATGGGG CATGAGTTTA ACAATCAGAG CCCCTAGCCT AGACTACCAA
    AACACTCTGG GACTTTGTGG CACCTTTGAT AATAACCCAG AAAATGATTT CCATAATAAA
    AATGGGATAA AAATTGATCT AACATTTAAT AATTGTGTTG CTTTTATTAA TGAGTGGAGG
    ATTTTGCCGG GGAAAAGCAT GTTTGACACA ATGCCAATTT CTCCGCCATC ACCTGGAAAA
    CTCTCCTATT GTAGCTGCTC ATTGAACACT AATTCATTAT ATCCTTTTGA TCACCTGGAC
    AGTGTATCTC AACAAGAGAT TATTTCAGGT TGCAAAGATC TCAAACATGC CAAATTCTCT
    TTCCTGATAC CAGAACTAGA TGTCACCTCT GAATATATTA ATTCAGAAGC TCTCATCAGA
    GGAATAAATG AGTATACATC TGGAGAAGAA AATAACTTGA ACTTCCTGCC TCAAGAAAAA
    AAGCATGTGA ACCTAAGCAA ACTGGAATTA CATTTACAAA AACATCCTGG AAATGAAAAA
    CAAGAGGCAC CGAAGACTTT GGACAACGAG AAACATACAC AGGACCAGGG GAGCCACAGC
    CAGGAAATGA GGCGGGATCC ACAAAACAGA TGGAAACGGC AAAACTTTTA TGAGTTTCTT
    CCTATGTTTG CTTTCCAGAG TCTCAGCCAA AGTGACCTAG AAGAATTGAC TTATTTTTTC
    CCTGAGGACC ATACTGAGGA TGTCCACCAG GAGTTTGTCC CTACGTGGCC CACACCCTCT
    GGTCTCACCG AAGGCCACAC TTTGGCACTG TGCCAGCAGA CTCTTGCCAA CTCTAGTGTA
    GGAAGACACT GTCTTGCCTT TCTGGGCAAG AGTCTGGACA ATGCTATAGA TATGTGTGTT
    AAGGATGTTC TCCTAAAAGA TGACCTCAGC TGGGCAGAAG CAGGTGTGGC TCTTTTAGAA
    AATGAATGTG AAAAGAGAAT TTTGGAAGAG GGGAAATACA ATGCAGAAGA AAATAGCAAG
    TCAGTCGAAG ATATTCTCTT GGTACTAAAA TGCCCCAATT TATGCAGCGG CCATGGGCAG
    TGCATGGAAT GGGGATGCGC ATGTTCCCCT GGCTTTGGTT CCTATGACTG CAGTGATTCG
    CATGACATGC CTCCTGAAAT TATAGAACTT GAGAATGCTG GATTCTGTAA TATTCGAAAA
    TATAATTGTA TGATAGTGAG AGTGTTTGGT AAAGGCTTCA AAGAATCACC TTCAATTAAA
    TGTGAAGTTA CTAAACTGCA GTATAATAGC AGTAAATGGG TCCTTGGAAA AACTGTCTAC
    ACGCATACTG TTTTTCAAAA TAGCAGAACT GTTGATTGTC AGCTGCCCCC TGATGTTCAA
    CCATCTGATA CCATGAATCT GATGGATGAG AAACCAATTG CGAAGTGGCA ATTAAAGGTA
    TCCAATGATG GTTATAGGTT CAGTAATCCC AAAATAATGA TCATTTATGA TGGAGCTTGT
    CAAATTTGTG GTCTCTATGG GAACGACTCA TGTGCTCTAA AGGAAAATGT TTGCATTATT
    GATGGACTCT GCTACGTTGA AGAAGATAAA AATCCTACCA GCCCTTGTTT GATTTGTAAA
    CCAAAAATGT CAAAATTTAC TTGGTCAGTT TTAGAAAACA ACCAACCTCC AGTGATTCAA
    ATACCGCAAG ACAAATTACA GACATTTTTC GGGGAGAACT TTTTGTACCA GTTCACGGCC
    TTTGATCCTG AAGGTTCTGA CGTCCGTTTT ACCTTGGACT CGGGTCCTGA GGGAGCAAAT
    GTTTCCTCTT CAGGACTTCT TATGTGGAAA ACAGAATCCC AAACTCCACA GGAATTCACT
    CTACGTTTTA ATGACGACTG TAATGCTGAA ACGAGAGTCA CGATTATGGT GACTGTGAAG
    TCTTGTGATT GCTTGAATGG TGGATTATGC GTGTCAGACA CTAAATCTCC TCCAGGAAGT
    GGAATGTACC TGTGTGTCTG CTTGCCTGGC TTTCAGGGCA GTCTTTGTGA AGTGAACGTC
    ACTGAGTGCC AGTCAAACCC CTGTGGTCTT GGCAAATGTG TTAGTGGATT TCAGAGTTAT
    TCTTGTGTAT GTCCACCTGA ACTCAAAGGC AAAACTTGCC AGGAGGATGT TGATGAGTGT
    GAATCCAGGC CTTGCTTTCC AGGAGCAGAA TGTCTCAATA CCTTTGGTTC CTATCATTGT
    GGTGCATGTC CAAAAGGATT TTATGGTGAT GGGAAAATAT GTCATGATAC AGCATTTCAG
    CCACCATCAA CAGATCACTC ACCATCTGGG GCAAACTTTA CCCACAGTCC TCCTACTGTC
    AAGAAGTTCT TGGACACACC CAGTAACATC TCACACTTCT CATTAAAATC AGTTACAACT
    CAAATGAATA ATTCAAAGAA AGTTCTTTCC CATCAAATAT CAGGCTTTGA ACAGATGGAT
    CATACTATCA GTACAAATCT CAGTCTGGAG GAATTGGGAG TTTCCATGAA CAACCATAAT
    TCTTTAAGCG TTAACCCAAA TCATTCCCCT GGTGAAGTAA AGGATTTTAT ACAGAGCACC
    ATCCAAACAG AGGCAGGAGG GCATAATTCT CAAATTAACA GGTCTGCCCA AAGTTACACG
    CAGTATAAAG ATAGGTATGC CTCATCAGTT ACTAGAGCCA TCACTGTTTT GCCAGAAAAG
    CCTGGATGGA TGAAAATATC AACTTGTGCT GGATCACCTT GTTTTCTCGG TGTTTCTTGT
    GTGCCAACCA CAGATGGTCA CTTCCAATGT GGACGCTGCC CCTTTGGATA TTATGGAGAT
    GGTATCAATT GCAGAGCCAT TTGTAGACAC CCATGTGGAA AAAGCAGGGA GTGTGTAGCC
    CCAAACATAT GCAAATGTAA ACCTGGTTAC ACTGGTTCTA ACTGCCAAAC TGCTGTATGT
    CATCCTGATT GCAAAAACCA TGGAAAATGT ATCAAGCCTA ACATTTGTGA CTGTCCCCCG
    GGACATGGGG GTGCAACCTG TGATGAAGAA CATTGTAGCC CACCTTGTCA ACATGGTGGC
    ACCTGCTTGG CTGGAAATCT CTGCACTTGT CCTTACGGTT TTATAGGACC AAGGTGTGAA
    ACAATGGTTT GTAACAGGCA CTGTGAAAAT GGAGGTGAAT GTCTTACACC AGATATTTGC
    CAGTGCAAGC CTGGCTGGTA TGGACCTACT TGTGGTACAG CTTTGTGTGA CCCCGTTTGC
    CTCAATGGTG GTTCCTGTAA TAAGCCAAAC ACTTGCCTCT GTCCAAATGG ATTCTTTGGT
    GCACAGTGTC AGAATGCTAT CTGTCACCCT CCCTGTAAGA ATGGTGGCCG CTGCATGAGA
    AATAATGTGT GTGCCTGTCG TGAAGGATAT GCTGGGAAGA GATGCCAAAA AAGGCTGTTT
    CATCCTTTTA CCTTAAGGTA TCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.