IBTK gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005627569.3	XP_005627626.1	inhibitor of Bruton tyrosine kinase isoform X3
XM_539018.5	XP_539018.2	inhibitor of Bruton tyrosine kinase isoform X2
XM_005627571.2	XP_005627628.1	inhibitor of Bruton tyrosine kinase isoform X5
XM_005627571.3	XP_005627628.1	inhibitor of Bruton tyrosine kinase isoform X5
XM_005627570.2	XP_005627627.1	inhibitor of Bruton tyrosine kinase isoform X4
XM_022426286.1	XP_022281994.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_539018.6	XP_539018.2	inhibitor of Bruton tyrosine kinase isoform X2
XM_005627570.3	XP_005627627.1	inhibitor of Bruton tyrosine kinase isoform X4
XM_022426287.1	XP_022281995.1	inhibitor of Bruton tyrosine kinase isoform X6
XM_014118123.1	XP_013973598.1	inhibitor of Bruton tyrosine kinase isoform X1
XM_005627569.2	XP_005627626.1	inhibitor of Bruton tyrosine kinase isoform X3
XM_014118123.2	XP_013973598.1	inhibitor of Bruton tyrosine kinase isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe292612	XM_022426287.1 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe39387	XM_022426286.1 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe39387	XM_014118123.2 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe15526	XM_005627571.3 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe15523	XM_539018.6 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe15524	XM_005627569.3 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe15525	XM_005627570.3 Latest version!	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OCe15526	XM_005627571.2	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe15523	XM_539018.5	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe15525	XM_005627570.2	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe15524	XM_005627569.2	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe39387	XM_014118123.1	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

IBTK ( XM_022426287.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281995.1 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe292612
Clone ID Related Accession (Same CDS sequence)	XM_022426287.1
Accession Version	XM_022426287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGTTCCTT TCTATGTGAT 
GTGACCATGA AATCAGTGGA TGGAAAGGAA TTTCCTTGTC ATAAGTGTGT TCTTTGTGCT 
AGACTTGAAT ATTTTCATAG TATGCTGAGT AGCTCATGGA TTGAGGCTTC TACTTGTACG 
GCTCTGGAAA TGCCAATACA TTCTGACATA CTGGAAGTTA TTTTGGACTA CCTCTATACT 
GATGAAGCTG TGGTGATAAA AGAATCTCAA AATGTGGATT TTGTTTGTAG TGTTCTCGTG 
GTGGCTGATC AGCTTCTTAT AACTCGATTG AAAGGGATTT GTGAAGTAGC ATTAACTGAG 
AAACTTACCC TTAAGAATGC TACTATGGTA CTGGAGTTTG CAGCAATGTA TAATGCTGAA 
CAATTGAAAC TGTCTTGTTT GCAGTTTATA GGACTGAATA TGGCCGCTTT ACTTGAAGCA 
AGGTCTCTTG ATGTTTTAAG TGATGGTGTT TTGAAGGATC TTTCTGTGTT TTATAGAAAA 
ATGATTCCAG CAATGGATAG AAGAGTCATT ACACCATACC AAGATGGACC AGATATTAGC 
TATTTGGAAG TAGAAGATGG AGATGTCTTT TTGAAAGAAG AAATAAATAT GGAACAAAAT 
TATTCGGAAA GTATGTTCAA GAAGGCAAAA ACAAAAGCAA AAAAGAAGCC GCGTAAGCGT 
TCAGATAGTT CTGGAGGTTA TAACCTTTCA GATGTTATTC AGAGTCCACC ATCTACAGGA 
TTATTAAAGT CTGGTAAGAA CAATTCTGTG GAATCTCTTC CAGAACTGTT GACATCAGAC 
TCTGAAGGAA GCTATGCAGG AGTGGGTAGT CCTAGAGATT TACAGTCCCC TGATTTCACA 
GCAGGATTTC AGTCAGATAA AATTGAGGTG AAAATTAAAC CATATGTTAA TGGTACACCT 
CCTGCATATT CTAGGGAAGA TTTAAAACCA TGGGAAAAAT CACCAATACT TAACATATCT 
GCTCCACAGA CCATTCCTAG TAACAGAATT GATACTACAA ACTCTTCTAG TTGGGTTGCT 
GGCTCTTTCA GTCCTGTCAG CCCTCCTGTT ATGGATCTCA GAACCATCAT GCAAATTGAA 
GAAAGTAGAC AAAAATGTGG AGCTACACCA AAGACAAATT TGGGCAAGAT GATTTCTCAT 
GGAGTTAAAC TTTCTCAGAA GCAACGGAAA ATGATTGCAA TGACTACCAA AGAAAACAAT 
TCAGGAACGA ATAGCATGGA AACAGCTTTA ACTGCTCCTT CAAAAACTCC AAAACCAGCA 
AACGCATGGG CATCTTCTGT ACATTCAGTT TCATCCAAGT CATTCCGGGA TTTCTTTGAA 
GAAAAAAAAT CTATTACTGG CCATAGTTCA GGAGATCATG TCAAAAAAGT TTGTTTTAAA 
GGAATAGAAA ATTCTCAGGC ATCAAAAGTT GAAAGATGTT CCCCTCATGG TACGCCAGGA 
CCAGAAGGCA ACCATATTTC AGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCT 
TCTTCATTGA CTGCTCCTCC TGTGGTAGCT CCAGTCACTT TTGCATCTAT TGTAGAAGAA 
GAATTACAGC AGGAAGCAGC TTTAATCAGA AGTCGAGAAA AACCCTTGGC TTTGATTCAG 
ATTGAAGAGC GTGCAATACA AGATTTATTG GTTTTCTATG AGGCATTTGG CAACCCTGAT 
GAGTTTGTTA TTGTTGAAAG GACACCACAG GGACCACTAG CAGTACCTAT GTGGAATAAG 
CATGGATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281995.1
CDS	556..4527
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSSSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_022426287.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426287.1

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGTTCCTT TCTATGTGAT 
GTGACCATGA AATCAGTGGA TGGAAAGGAA TTTCCTTGTC ATAAGTGTGT TCTTTGTGCT 
AGACTTGAAT ATTTTCATAG TATGCTGAGT AGCTCATGGA TTGAGGCTTC TACTTGTACG 
GCTCTGGAAA TGCCAATACA TTCTGACATA CTGGAAGTTA TTTTGGACTA CCTCTATACT 
GATGAAGCTG TGGTGATAAA AGAATCTCAA AATGTGGATT TTGTTTGTAG TGTTCTCGTG 
GTGGCTGATC AGCTTCTTAT AACTCGATTG AAAGGGATTT GTGAAGTAGC ATTAACTGAG 
AAACTTACCC TTAAGAATGC TACTATGGTA CTGGAGTTTG CAGCAATGTA TAATGCTGAA 
CAATTGAAAC TGTCTTGTTT GCAGTTTATA GGACTGAATA TGGCCGCTTT ACTTGAAGCA 
AGGTCTCTTG ATGTTTTAAG TGATGGTGTT TTGAAGGATC TTTCTGTGTT TTATAGAAAA 
ATGATTCCAG CAATGGATAG AAGAGTCATT ACACCATACC AAGATGGACC AGATATTAGC 
TATTTGGAAG TAGAAGATGG AGATGTCTTT TTGAAAGAAG AAATAAATAT GGAACAAAAT 
TATTCGGAAA GTATGTTCAA GAAGGCAAAA ACAAAAGCAA AAAAGAAGCC GCGTAAGCGT 
TCAGATAGTT CTGGAGGTTA TAACCTTTCA GATGTTATTC AGAGTCCACC ATCTACAGGA 
TTATTAAAGT CTGGTAAGAA CAATTCTGTG GAATCTCTTC CAGAACTGTT GACATCAGAC 
TCTGAAGGAA GCTATGCAGG AGTGGGTAGT CCTAGAGATT TACAGTCCCC TGATTTCACA 
GCAGGATTTC AGTCAGATAA AATTGAGGTG AAAATTAAAC CATATGTTAA TGGTACACCT 
CCTGCATATT CTAGGGAAGA TTTAAAACCA TGGGAAAAAT CACCAATACT TAACATATCT 
GCTCCACAGA CCATTCCTAG TAACAGAATT GATACTACAA ACTCTTCTAG TTGGGTTGCT 
GGCTCTTTCA GTCCTGTCAG CCCTCCTGTT ATGGATCTCA GAACCATCAT GCAAATTGAA 
GAAAGTAGAC AAAAATGTGG AGCTACACCA AAGACAAATT TGGGCAAGAT GATTTCTCAT 
GGAGTTAAAC TTTCTCAGAA GCAACGGAAA ATGATTGCAA TGACTACCAA AGAAAACAAT 
TCAGGAACGA ATAGCATGGA AACAGCTTTA ACTGCTCCTT CAAAAACTCC AAAACCAGCA 
AACGCATGGG CATCTTCTGT ACATTCAGTT TCATCCAAGT CATTCCGGGA TTTCTTTGAA 
GAAAAAAAAT CTATTACTGG CCATAGTTCA GGAGATCATG TCAAAAAAGT TTGTTTTAAA 
GGAATAGAAA ATTCTCAGGC ATCAAAAGTT GAAAGATGTT CCCCTCATGG TACGCCAGGA 
CCAGAAGGCA ACCATATTTC AGATTTACCA CTTCTAGACA GTCCCAATCC CTGGCTGTCT 
TCTTCATTGA CTGCTCCTCC TGTGGTAGCT CCAGTCACTT TTGCATCTAT TGTAGAAGAA 
GAATTACAGC AGGAAGCAGC TTTAATCAGA AGTCGAGAAA AACCCTTGGC TTTGATTCAG 
ATTGAAGAGC GTGCAATACA AGATTTATTG GTTTTCTATG AGGCATTTGG CAACCCTGAT 
GAGTTTGTTA TTGTTGAAAG GACACCACAG GGACCACTAG CAGTACCTAT GTGGAATAAG 
CATGGATGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_022426286.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281994.1 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe39387
Clone ID Related Accession (Same CDS sequence)	XM_022426286.1 , XM_014118123.1 , XM_014118123.2
Accession Version	XM_022426286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT 
CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA 
GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT 
CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT 
TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA 
CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281994.1
CDS	608..4666
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_022426286.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022426286.1

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT 
CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA 
GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT 
CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT 
TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA 
CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_014118123.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973598.1 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X1, mRNA.

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CloneID	OCe39387
Clone ID Related Accession (Same CDS sequence)	XM_022426286.1 , XM_014118123.1 , XM_014118123.2
Accession Version	XM_014118123.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT 
CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA 
GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT 
CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT 
TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA 
CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973598.1
CDS	1231..5289
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_014118123.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118123.1

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT 
CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA 
GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT 
CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT 
TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA 
CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_014118123.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013973598.1 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA.

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CloneID	OCe39387
Clone ID Related Accession (Same CDS sequence)	XM_022426286.1 , XM_014118123.1 , XM_014118123.2
Accession Version	XM_014118123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014118123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT 
CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA 
GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT 
CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT 
TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA 
CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013973598.1
CDS	556..4614
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_014118123.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014118123.2

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGATGTTCCC CTCATGGTAC GCCAGGACCA GAAGGCAACC ATATTTCAGA TTTACCACTT 
CTAGACAGTC CCAATCCCTG GCTGTCTTCT TCATTGACTG CTCCTCCTGT GGTAGCTCCA 
GTCACTTTTG CATCTATTGT AGAAGAAGAA TTACAGCAGG AAGCAGCTTT AATCAGAAGT 
CGAGAAAAAC CCTTGGCTTT GATTCAGATT GAAGAGCGTG CAATACAAGA TTTATTGGTT 
TTCTATGAGG CATTTGGCAA CCCTGATGAG TTTGTTATTG TTGAAAGGAC ACCACAGGGA 
CCACTAGCAG TACCTATGTG GAATAAGCAT GGATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_005627571.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627628.1 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe15526
Clone ID Related Accession (Same CDS sequence)	XM_005627571.2 , XM_005627571.3
Accession Version	XM_005627571.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inhibitor of Bruton tyrosine kinase isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627571.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA 
TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC 
TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA 
TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA 
CCTATGTGGA ATAAGCATGG ATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627628.1
CDS	1231..5217
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVESPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_005627571.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627571.2

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA 
TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC 
TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA 
TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA 
CCTATGTGGA ATAAGCATGG ATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_005627571.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627628.1 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X6, mRNA.

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CloneID	OCe15526
Clone ID Related Accession (Same CDS sequence)	XM_005627571.2 , XM_005627571.3
Accession Version	XM_005627571.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627571.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA 
TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC 
TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA 
TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA 
CCTATGTGGA ATAAGCATGG ATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627628.1
CDS	556..4542
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVESPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_005627571.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627571.3

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGGATTA TTAAAGTCTG GTAAGAACAA TTCTGTGGAA 
TCTCTTCCAG AACTGTTGAC ATCAGACTCT GAAGGAAGCT ATGCAGGAGT GGGTAGTCCT 
AGAGATTTAC AGTCCCCTGA TTTCACAGCA GGATTTCAGT CAGATAAAAT TGAGGTGAAA 
ATTAAACCAT ATGTTAATGG TACACCTCCT GCATATTCTA GGGAAGATTT AAAACCATGG 
GAAAAATCAC CAATACTTAA CATATCTGCT CCACAGACCA TTCCTAGTAA CAGAATTGAT 
ACTACAAACT CTTCTAGTTG GGTTGCTGGC TCTTTCAGTC CTGTCAGCCC TCCTGTTATG 
GATCTCAGAA CCATCATGCA AATTGAAGAA AGTAGACAAA AATGTGGAGC TACACCAAAG 
ACAAATTTGG GCAAGATGAT TTCTCATGGA GTTAAACTTT CTCAGAAGCA ACGGAAAATG 
ATTGCAATGA CTACCAAAGA AAACAATTCA GGAACGAATA GCATGGAAAC AGCTTTAACT 
GCTCCTTCAA AAACTCCAAA ACCAGCAAAC GCATGGGCAT CTTCTGTACA TTCAGTTTCA 
TCCAAGTCAT TCCGGGATTT CTTTGAAGAA AAAAAATCTA TTACTGGCCA TAGTTCAGGA 
GATCATGTCA AAAAAGTTTG TTTTAAAGGA ATAGAAAATT CTCAGGCATC AAAAGTTGAA 
AGTCCCTGGC TGTCTTCTTC ATTGACTGCT CCTCCTGTGG TAGCTCCAGT CACTTTTGCA 
TCTATTGTAG AAGAAGAATT ACAGCAGGAA GCAGCTTTAA TCAGAAGTCG AGAAAAACCC 
TTGGCTTTGA TTCAGATTGA AGAGCGTGCA ATACAAGATT TATTGGTTTT CTATGAGGCA 
TTTGGCAACC CTGATGAGTT TGTTATTGTT GAAAGGACAC CACAGGGACC ACTAGCAGTA 
CCTATGTGGA ATAAGCATGG ATGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_539018.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539018.2 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X2, mRNA.

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CloneID	OCe15523
Clone ID Related Accession (Same CDS sequence)	XM_539018.5 , XM_539018.6
Accession Version	XM_539018.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inhibitor of Bruton tyrosine kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_539018.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT 
CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA 
GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT 
AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA 
AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT 
ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT 
CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA 
AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT 
CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC 
AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA 
TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA 
GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC 
ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA 
GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC 
TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA 
CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539018.2
CDS	1231..5286
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_539018.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539018.5

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT 
CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA 
GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT 
AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA 
AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT 
ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT 
CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA 
AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT 
CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC 
AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA 
TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA 
GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC 
ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA 
GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC 
TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA 
CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_539018.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_539018.2 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe15523
Clone ID Related Accession (Same CDS sequence)	XM_539018.5 , XM_539018.6
Accession Version	XM_539018.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539018.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT 
CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA 
GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT 
AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA 
AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT 
ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT 
CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA 
AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT 
CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC 
AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA 
TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA 
GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC 
ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA 
GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC 
TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA 
CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539018.2
CDS	556..4611
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTGLLKSGKNNSVESLPELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_539018.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539018.6

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGGATTATTA AAGTCTGGTA AGAACAATTC TGTGGAATCT 
CTTCCAGAAC TGTTGACATC AGACTCTGAA GGAAGCTATG CAGGAGTGGG TAGTCCTAGA 
GATTTACAGT CCCCTGATTT CACAGCAGGA TTTCAGTCAG ATAAAATTGA GGTGAAAATT 
AAACCATATG TTAATGGTAC ACCTCCTGCA TATTCTAGGG AAGATTTAAA ACCATGGGAA 
AAATCACCAA TACTTAACAT ATCTGCTCCA CAGACCATTC CTAGTAACAG AATTGATACT 
ACAAACTCTT CTAGTTGGGT TGCTGGCTCT TTCAGTCCTG TCAGCCCTCC TGTTATGGAT 
CTCAGAACCA TCATGCAAAT TGAAGAAAGT AGACAAAAAT GTGGAGCTAC ACCAAAGACA 
AATTTGGGCA AGATGATTTC TCATGGAGTT AAACTTTCTC AGAAGCAACG GAAAATGATT 
GCAATGACTA CCAAAGAAAA CAATTCAGGA ACGAATAGCA TGGAAACAGC TTTAACTGCT 
CCTTCAAAAA CTCCAAAACC AGCAAACGCA TGGGCATCTT CTGTACATTC AGTTTCATCC 
AAGTCATTCC GGGATTTCTT TGAAGAAAAA AAATCTATTA CTGGCCATAG TTCAGGAGAT 
CATGTCAAAA AAGTTTGTTT TAAAGGAATA GAAAATTCTC AGGCATCAAA AGTTGAAAGA 
TGTTCCCCTC ATGGTACGCC AGGACCAGAA GGCAACCATA TTTCAGATTT ACCACTTCTA 
GACAGTCCCA ATCCCTGGCT GTCTTCTTCA TTGACTGCTC CTCCTGTGGT AGCTCCAGTC 
ACTTTTGCAT CTATTGTAGA AGAAGAATTA CAGCAGGAAG CAGCTTTAAT CAGAAGTCGA 
GAAAAACCCT TGGCTTTGAT TCAGATTGAA GAGCGTGCAA TACAAGATTT ATTGGTTTTC 
TATGAGGCAT TTGGCAACCC TGATGAGTTT GTTATTGTTG AAAGGACACC ACAGGGACCA 
CTAGCAGTAC CTATGTGGAA TAAGCATGGA TGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_005627569.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627626.1 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe15524
Clone ID Related Accession (Same CDS sequence)	XM_005627569.3 , XM_005627569.2
Accession Version	XM_005627569.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627569.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA 
GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT 
AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA 
GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT 
AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC 
AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT 
GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG 
AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG 
GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT 
GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT 
GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG 
GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT 
TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT 
CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA 
GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA 
CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA 
AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627626.1
CDS	556..4569
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_005627569.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627569.3

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA 
GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT 
AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA 
GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT 
AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC 
AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT 
GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG 
AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG 
GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT 
GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT 
GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG 
GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT 
TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT 
CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA 
GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA 
CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA 
AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_005627569.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627626.1 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X3, mRNA.

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CloneID	OCe15524
Clone ID Related Accession (Same CDS sequence)	XM_005627569.3 , XM_005627569.2
Accession Version	XM_005627569.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inhibitor of Bruton tyrosine kinase isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA 
GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT 
AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA 
GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT 
AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC 
AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT 
GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG 
AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG 
GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT 
GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT 
GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG 
GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT 
TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT 
CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA 
GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA 
CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA 
AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627626.1
CDS	1231..5244
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKAEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_005627569.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627569.2

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGCAGA GATTTTATCA 
AATCTTCATA ATTCCTCATC AGATGTGTCT TGTGTCTCTG ATATAAATAG TGTATACGAA 
AGAATTCGAC TTGAGAAACT TACTTTTGCC CATAGAGCTG TTAGTGTCAG CACAGATCCA 
AGTGGATGCA ACTTTGCAAT CCTGCAGTCA GATCCTAAAA CAAGCCTTTA TGAAATTCCA 
GCCGTATCCT CATCATCCTT TTTTGAAGAG TTTGGCAAAC TCTTAAGGGA AACAGATGAA 
ACGGACAGTA TTCATGATGT GACATTTCAA GTTGGCAATA GAATTTTCCC TGCACATAAA 
TATATTTTGG CAGTGCGTTC TGACTTTTTT CAGAAATTGT TTCTTTCAGA TGGGACTACT 
TTAGATTTTA TAGATGTTTA CCGGAAAGAT GAAGATTCTG CAGGATGCCA TCTCTTTGTC 
ATAGAGAAGG TTCATCCTGA CTTGTTTGAG TACCTTTTAC AATTTATATA CACAGATACT 
TGTGACTTTT TAATTCATGG CTTCACACCA AGAATAAACT TAAACAAAAA GCCAGAAGAA 
TATCAGGGCA CTATGAATTC ACATTCAAAT AAAATGCATT GCCATGAAGA TAATCAGAAG 
TCAGCATTTG AAGTTTACAG AAGTAATCAA GCACACACAG TTAATGAAAA GCAAAAAAGC 
AAATCAAAGG CTTCCAATAA AGGGAAAAGT ATTGGGGAAG ATGATCCTGT AAGAATGTTG 
CAAAATGTTG CAAAGAAATT TGGTTTCAGT AATTTGAGTA GTAGGTTAGA TGGAGTCAGA 
TTTGAAAATG GAAAAATTAA TGTTATTGAG AAGAAAACTG GTAATAAATC AAAGCTAAAT 
CAGAAAAAAT GTTCCTTTCT ATGTGATGTG ACCATGAAAT CAGTGGATGG AAAGGAATTT 
CCTTGTCATA AGTGTGTTCT TTGTGCTAGA CTTGAATATT TTCATAGTAT GCTGAGTAGC 
TCATGGATTG AGGCTTCTAC TTGTACGGCT CTGGAAATGC CAATACATTC TGACATACTG 
GAAGTTATTT TGGACTACCT CTATACTGAT GAAGCTGTGG TGATAAAAGA ATCTCAAAAT 
GTGGATTTTG TTTGTAGTGT TCTCGTGGTG GCTGATCAGC TTCTTATAAC TCGATTGAAA 
GGGATTTGTG AAGTAGCATT AACTGAGAAA CTTACCCTTA AGAATGCTAC TATGGTACTG 
GAGTTTGCAG CAATGTATAA TGCTGAACAA TTGAAACTGT CTTGTTTGCA GTTTATAGGA 
CTGAATATGG CCGCTTTACT TGAAGCAAGG TCTCTTGATG TTTTAAGTGA TGGTGTTTTG 
AAGGATCTTT CTGTGTTTTA TAGAAAAATG ATTCCAGCAA TGGATAGAAG AGTCATTACA 
CCATACCAAG ATGGACCAGA TATTAGCTAT TTGGAAGTAG AAGATGGAGA TGTCTTTTTG 
AAAGAAGAAA TAAATATGGA ACAAAATTAT TCGGAAAGTA TGTTCAAGAA GGCAAAAACA 
AAAGCAAAAA AGAAGCCGCG TAAGCGTTCA GATAGTTCTG GAGGTTATAA CCTTTCAGAT 
GTTATTCAGA GTCCACCATC TACAGAACTG TTGACATCAG ACTCTGAAGG AAGCTATGCA 
GGAGTGGGTA GTCCTAGAGA TTTACAGTCC CCTGATTTCA CAGCAGGATT TCAGTCAGAT 
AAAATTGAGG TGAAAATTAA ACCATATGTT AATGGTACAC CTCCTGCATA TTCTAGGGAA 
GATTTAAAAC CATGGGAAAA ATCACCAATA CTTAACATAT CTGCTCCACA GACCATTCCT 
AGTAACAGAA TTGATACTAC AAACTCTTCT AGTTGGGTTG CTGGCTCTTT CAGTCCTGTC 
AGCCCTCCTG TTATGGATCT CAGAACCATC ATGCAAATTG AAGAAAGTAG ACAAAAATGT 
GGAGCTACAC CAAAGACAAA TTTGGGCAAG ATGATTTCTC ATGGAGTTAA ACTTTCTCAG 
AAGCAACGGA AAATGATTGC AATGACTACC AAAGAAAACA ATTCAGGAAC GAATAGCATG 
GAAACAGCTT TAACTGCTCC TTCAAAAACT CCAAAACCAG CAAACGCATG GGCATCTTCT 
GTACATTCAG TTTCATCCAA GTCATTCCGG GATTTCTTTG AAGAAAAAAA ATCTATTACT 
GGCCATAGTT CAGGAGATCA TGTCAAAAAA GTTTGTTTTA AAGGAATAGA AAATTCTCAG 
GCATCAAAAG TTGAAAGATG TTCCCCTCAT GGTACGCCAG GACCAGAAGG CAACCATATT 
TCAGATTTAC CACTTCTAGA CAGTCCCAAT CCCTGGCTGT CTTCTTCATT GACTGCTCCT 
CCTGTGGTAG CTCCAGTCAC TTTTGCATCT ATTGTAGAAG AAGAATTACA GCAGGAAGCA 
GCTTTAATCA GAAGTCGAGA AAAACCCTTG GCTTTGATTC AGATTGAAGA GCGTGCAATA 
CAAGATTTAT TGGTTTTCTA TGAGGCATTT GGCAACCCTG ATGAGTTTGT TATTGTTGAA 
AGGACACCAC AGGGACCACT AGCAGTACCT ATGTGGAATA AGCATGGATG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_005627570.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627627.1 Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe15525
Clone ID Related Accession (Same CDS sequence)	XM_005627570.2 , XM_005627570.3
Accession Version	XM_005627570.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inhibitor of Bruton tyrosine kinase isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005627570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA 
GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA 
ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT 
TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT 
AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC 
CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA 
GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG 
CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA 
ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA 
CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC 
CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA 
TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA 
GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT 
GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT 
TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA 
GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG 
ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627627.1
CDS	1231..5241
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_005627570.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris inhibitor of Bruton agammaglobulinemia tyrosine kinase (IBTK), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627570.2

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA 
GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA 
ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT 
TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT 
AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC 
CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA 
GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG 
CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA 
ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA 
CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC 
CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA 
TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA 
GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT 
GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT 
TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA 
GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG 
ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IBTK ( XM_005627570.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005627627.1 Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe15525
Clone ID Related Accession (Same CDS sequence)	XM_005627570.2 , XM_005627570.3
Accession Version	XM_005627570.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inhibitor of Bruton tyrosine kinase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005627570.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA 
GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA 
ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT 
TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT 
AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC 
CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA 
GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG 
CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA 
ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA 
CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC 
CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA 
TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA 
GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT 
GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT 
TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA 
GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG 
ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005627627.1
CDS	556..4566
Translation	MNSSIPDCTSKCRSLKHALDVLSVVTKGNENQIKAFLSSYCYNAATIKDAFGRNALHLVSSCGKKGVLDWLIEKGVDLLVKDKESGWTALHRSIFYGHIDCVWSLLKHGVSLYIQDKEGLSALDLVMKDRPTHVVFKTTDPTDVYTWGDNTNFTLGHGSQNSKHHPELVDLFSRSGVYIKQVVLCKFHSVFLSQKGQVYTCGHGPGGRLGHGDEQTCLIPRLVEGLSGHTCSQVAAAKDHTVVLTEDGCVYTFGLNIFHQLGIIPPPSSCNVPRQMQAKYLKGRTIIGVAAGRFHTVLWTREAVYTMGLNGGQLGYLPDPNGEKYVTAPRQVSALHHKDITLSLVAASDGATVCVTTRGDIYLLADYQCKKMASKQLNLKKVLVSGGRMEYKVDPENLKENGGQKICILAMDGAGRVFCWRSVSSSLKQCRWAYPRQVFISDIALNRKDILFVTQDGEGFRGKWFEEKKRNSEKKEILSNLHNSSSDVSCVSDINSVYERIRLEKLTFAHRAVSVSTDPSGCNFAILQSDPKTSLYEIPAVSSSSFFEEFGKLLRETDETDSIHDVTFQVGNRIFPAHKYILAVRSDFFQKLFLSDGTTLDFIDVYRKDEDSAGCHLFVIEKVHPDLFEYLLQFIYTDTCDFLIHGFTPRINLNKKPEEYQGTMNSHSNKMHCHEDNQKSAFEVYRSNQAHTVNEKQKSKSKASNKGKSIGEDDPVRMLQNVAKKFGFSNLSSRLDGVRFENGKINVIEKKTGNKSKLNQKKCSFLCDVTMKSVDGKEFPCHKCVLCARLEYFHSMLSSSWIEASTCTALEMPIHSDILEVILDYLYTDEAVVIKESQNVDFVCSVLVVADQLLITRLKGICEVALTEKLTLKNATMVLEFAAMYNAEQLKLSCLQFIGLNMAALLEARSLDVLSDGVLKDLSVFYRKMIPAMDRRVITPYQDGPDISYLEVEDGDVFLKEEINMEQNYSESMFKKAKTKAKKKPRKRSDSSGGYNLSDVIQSPPSTELLTSDSEGSYAGVGSPRDLQSPDFTAGFQSDKIEVKIKPYVNGTPPAYSREDLKPWEKSPILNISAPQTIPSNRIDTTNSSSWVAGSFSPVSPPVMDLRTIMQIEESRQKCGATPKTNLGKMISHGVKLSQKQRKMIAMTTKENNSGTNSMETALTAPSKTPKPANAWASSVHSVSSKSFRDFFEEKKSITGHSSGDHVKKVCFKGIENSQASKVERCSPHGTPGPEGNHISDLPLLDSPNPWLSSSLTAPPVVAPVTFASIVEEELQQEAALIRSREKPLALIQIEERAIQDLLVFYEAFGNPDEFVIVERTPQGPLAVPMWNKHGC

Target ORF information:

RefSeq Version	XM_005627570.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris inhibitor of Bruton tyrosine kinase (IBTK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005627570.3

ORF Insert Sequence:

ATGAATTCTT CCATCCCTGA CTGCACATCA AAGTGTCGAT CCCTGAAGCA TGCTTTGGAT 
GTCCTTTCTG TCGTAACAAA GGGGAATGAA AACCAGATTA AGGCCTTTCT CTCCAGTTAT 
TGTTACAATG CTGCAACTAT CAAAGATGCC TTTGGCAGAA ACGCCCTCCA CCTTGTTTCC 
TCCTGTGGAA AGAAAGGAGT ATTAGATTGG CTTATTGAGA AAGGAGTGGA TCTGCTGGTG 
AAAGACAAGG AATCAGGATG GACAGCTTTG CACAGAAGCA TTTTTTATGG ACATATTGAT 
TGTGTTTGGT CTCTCTTGAA GCATGGTGTT AGTCTGTATA TTCAAGACAA AGAAGGCTTG 
TCAGCTTTGG ATCTTGTAAT GAAGGACAGA CCAACTCATG TAGTATTCAA AACTACTGAT 
CCTACAGATG TCTACACCTG GGGAGATAAT ACAAATTTCA CCCTGGGTCA TGGAAGCCAG 
AATAGCAAAC ACCATCCAGA GTTGGTGGAT CTGTTCTCCA GGAGTGGGGT TTATATCAAG 
CAGGTGGTGC TTTGTAAATT TCATTCAGTG TTTCTTTCTC AGAAAGGGCA GGTTTATACC 
TGTGGTCATG GTCCTGGAGG ACGTTTAGGC CATGGAGATG AACAGACATG CTTGATTCCT 
AGGCTTGTGG AAGGACTGAG TGGTCATACT TGTTCCCAAG TGGCAGCCGC TAAGGATCAT 
ACTGTTGTAC TGACTGAAGA TGGATGTGTT TATACATTTG GTCTGAATAT TTTTCATCAA 
TTAGGAATTA TTCCTCCACC TTCCAGTTGT AATGTACCCA GACAGATGCA GGCAAAATAT 
CTGAAAGGAA GGACAATCAT TGGAGTAGCG GCAGGCAGGT TTCATACAGT GCTGTGGACT 
AGAGAAGCTG TTTACACTAT GGGACTAAAT GGTGGACAAC TGGGTTATTT GCCAGATCCC 
AATGGAGAAA AGTATGTAAC TGCTCCTCGT CAAGTCTCTG CCCTTCATCA TAAGGATATC 
ACTTTGTCTT TAGTTGCTGC AAGTGATGGG GCCACAGTCT GTGTTACCAC AAGGGGAGAT 
ATTTACTTAC TTGCAGACTA TCAGTGCAAG AAGATGGCTT CTAAACAACT AAACTTGAAA 
AAAGTTCTTG TTTCTGGGGG ACGTATGGAA TACAAGGTTG ATCCTGAAAA TTTGAAGGAA 
AATGGGGGTC AAAAAATTTG CATTCTTGCC ATGGATGGAG CTGGAAGGGT ATTTTGCTGG 
AGATCAGTCA GCAGTTCTCT AAAGCAGTGT CGATGGGCTT ATCCACGCCA GGTCTTCATT 
TCTGATATTG CTTTAAATAG AAAAGACATT TTATTTGTCA CACAAGATGG AGAAGGATTT 
AGAGGGAAAT GGTTTGAAGA AAAAAAAAGG AATTCTGAAA AGAAAGAGAT TTTATCAAAT 
CTTCATAATT CCTCATCAGA TGTGTCTTGT GTCTCTGATA TAAATAGTGT ATACGAAAGA 
ATTCGACTTG AGAAACTTAC TTTTGCCCAT AGAGCTGTTA GTGTCAGCAC AGATCCAAGT 
GGATGCAACT TTGCAATCCT GCAGTCAGAT CCTAAAACAA GCCTTTATGA AATTCCAGCC 
GTATCCTCAT CATCCTTTTT TGAAGAGTTT GGCAAACTCT TAAGGGAAAC AGATGAAACG 
GACAGTATTC ATGATGTGAC ATTTCAAGTT GGCAATAGAA TTTTCCCTGC ACATAAATAT 
ATTTTGGCAG TGCGTTCTGA CTTTTTTCAG AAATTGTTTC TTTCAGATGG GACTACTTTA 
GATTTTATAG ATGTTTACCG GAAAGATGAA GATTCTGCAG GATGCCATCT CTTTGTCATA 
GAGAAGGTTC ATCCTGACTT GTTTGAGTAC CTTTTACAAT TTATATACAC AGATACTTGT 
GACTTTTTAA TTCATGGCTT CACACCAAGA ATAAACTTAA ACAAAAAGCC AGAAGAATAT 
CAGGGCACTA TGAATTCACA TTCAAATAAA ATGCATTGCC ATGAAGATAA TCAGAAGTCA 
GCATTTGAAG TTTACAGAAG TAATCAAGCA CACACAGTTA ATGAAAAGCA AAAAAGCAAA 
TCAAAGGCTT CCAATAAAGG GAAAAGTATT GGGGAAGATG ATCCTGTAAG AATGTTGCAA 
AATGTTGCAA AGAAATTTGG TTTCAGTAAT TTGAGTAGTA GGTTAGATGG AGTCAGATTT 
GAAAATGGAA AAATTAATGT TATTGAGAAG AAAACTGGTA ATAAATCAAA GCTAAATCAG 
AAAAAATGTT CCTTTCTATG TGATGTGACC ATGAAATCAG TGGATGGAAA GGAATTTCCT 
TGTCATAAGT GTGTTCTTTG TGCTAGACTT GAATATTTTC ATAGTATGCT GAGTAGCTCA 
TGGATTGAGG CTTCTACTTG TACGGCTCTG GAAATGCCAA TACATTCTGA CATACTGGAA 
GTTATTTTGG ACTACCTCTA TACTGATGAA GCTGTGGTGA TAAAAGAATC TCAAAATGTG 
GATTTTGTTT GTAGTGTTCT CGTGGTGGCT GATCAGCTTC TTATAACTCG ATTGAAAGGG 
ATTTGTGAAG TAGCATTAAC TGAGAAACTT ACCCTTAAGA ATGCTACTAT GGTACTGGAG 
TTTGCAGCAA TGTATAATGC TGAACAATTG AAACTGTCTT GTTTGCAGTT TATAGGACTG 
AATATGGCCG CTTTACTTGA AGCAAGGTCT CTTGATGTTT TAAGTGATGG TGTTTTGAAG 
GATCTTTCTG TGTTTTATAG AAAAATGATT CCAGCAATGG ATAGAAGAGT CATTACACCA 
TACCAAGATG GACCAGATAT TAGCTATTTG GAAGTAGAAG ATGGAGATGT CTTTTTGAAA 
GAAGAAATAA ATATGGAACA AAATTATTCG GAAAGTATGT TCAAGAAGGC AAAAACAAAA 
GCAAAAAAGA AGCCGCGTAA GCGTTCAGAT AGTTCTGGAG GTTATAACCT TTCAGATGTT 
ATTCAGAGTC CACCATCTAC AGAACTGTTG ACATCAGACT CTGAAGGAAG CTATGCAGGA 
GTGGGTAGTC CTAGAGATTT ACAGTCCCCT GATTTCACAG CAGGATTTCA GTCAGATAAA 
ATTGAGGTGA AAATTAAACC ATATGTTAAT GGTACACCTC CTGCATATTC TAGGGAAGAT 
TTAAAACCAT GGGAAAAATC ACCAATACTT AACATATCTG CTCCACAGAC CATTCCTAGT 
AACAGAATTG ATACTACAAA CTCTTCTAGT TGGGTTGCTG GCTCTTTCAG TCCTGTCAGC 
CCTCCTGTTA TGGATCTCAG AACCATCATG CAAATTGAAG AAAGTAGACA AAAATGTGGA 
GCTACACCAA AGACAAATTT GGGCAAGATG ATTTCTCATG GAGTTAAACT TTCTCAGAAG 
CAACGGAAAA TGATTGCAAT GACTACCAAA GAAAACAATT CAGGAACGAA TAGCATGGAA 
ACAGCTTTAA CTGCTCCTTC AAAAACTCCA AAACCAGCAA ACGCATGGGC ATCTTCTGTA 
CATTCAGTTT CATCCAAGTC ATTCCGGGAT TTCTTTGAAG AAAAAAAATC TATTACTGGC 
CATAGTTCAG GAGATCATGT CAAAAAAGTT TGTTTTAAAG GAATAGAAAA TTCTCAGGCA 
TCAAAAGTTG AAAGATGTTC CCCTCATGGT ACGCCAGGAC CAGAAGGCAA CCATATTTCA 
GATTTACCAC TTCTAGACAG TCCCAATCCC TGGCTGTCTT CTTCATTGAC TGCTCCTCCT 
GTGGTAGCTC CAGTCACTTT TGCATCTATT GTAGAAGAAG AATTACAGCA GGAAGCAGCT 
TTAATCAGAA GTCGAGAAAA ACCCTTGGCT TTGATTCAGA TTGAAGAGCG TGCAATACAA 
GATTTATTGG TTTTCTATGA GGCATTTGGC AACCCTGATG AGTTTGTTAT TGTTGAAAGG 
ACACCACAGG GACCACTAGC AGTACCTATG TGGAATAAGC ATGGATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	IBTK
Entrez Gene ID	481897
Full Name	inhibitor of Bruton tyrosine kinase
General protein information	Preferred Names inhibitor of Bruton tyrosine kinase Names inhibitor of Bruton tyrosine kinase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

	IBTK	XP_002803869.1
Bos taurus (cattle)	IBTK	NP_001192969.1
Drosophila melanogaster (fruit fly)	CG8060	NP_611117.2
Pan troglodytes (chimpanzee)	IBTK	XP_001148806.1
Xenopus tropicalis (tropical clawed frog)	ibtk	XP_002938503.2
Canis lupus familiaris (dog)	IBTK	XP_539018.2
Mus musculus (house mouse)	Ibtk	NP_001074751.1
Rattus norvegicus (Norway rat)	Ibtk	XP_006243569.1
Gallus gallus (chicken)	IBTK	XP_004940475.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009939	XP_319065.4
Homo sapiens (human)	IBTK	NP_056340.2
Danio rerio (zebrafish)	ibtk	XP_005157916.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

IBTK cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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