GNE cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GNE
Entrez Gene ID	481607
Full Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005626809.3	XP_005626866.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
XM_005626809.2	XP_005626866.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
XM_003431575.3	XP_003431623.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
XM_005626808.2	XP_005626865.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
XM_003431575.4	XP_003431623.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
XM_022425601.1	XP_022281309.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GNE	NP_001121699.1
Mus musculus (house mouse)	Gne	NP_056643.3
Rattus norvegicus (Norway rat)	Gne	NP_446217.1
	GNE	XP_001082113.2
Gallus gallus (chicken)	GNE	NP_001026603.2
Xenopus tropicalis (tropical clawed frog)	gne	NP_001072728.1
Canis lupus familiaris (dog)	GNE	XP_003431623.1
Danio rerio (zebrafish)	gne	NP_957177.1
Pan troglodytes (chimpanzee)	GNE	XP_003312121.1
Bos taurus (cattle)	GNE	NP_001178072.2

Gene Ontology

The following GNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe14623	XM_003431575.4 Latest version!	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe14625	XM_005626808.2 Latest version!	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe14625	XM_005626809.3 Latest version!	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe292175	XM_022425601.1 Latest version!	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe14625	XM_005626809.2	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OCe14623	XM_003431575.3	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNE ( XM_003431575.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003431623.1 Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14623
Clone ID Related Accession (Same CDS sequence)	XM_003431575.3 , XM_003431575.4
Accession Version	XM_003431575.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003431575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCA ATGTGTATCC TCAGTGGAAG TTGTGCCTTC AAGGACCTCA TGAATTCTAT 
TTTAAGAACC TCTCACAAAA GAGACGAGCC ATGGAGAAGA ATGGGGATAA CCGGAAGCTT 
CGAGTTTGTG TCGCCACTTG CAACCGTGCT GATTATTCCA AACTTGCCCC AATCATGTTT 
GGCATTAAAA TGGAACCTGA GTTCTTTGAA CTTGATGTGG TGGTACTTGG CTCTCACCTA 
ATAGATGATT ATGGGAACAC ATACCGCATG ATTGAACAAG ATGACTTTGA CATTAATACC 
AGGCTACACA CAATTGTGAG AGGGGAAGAT GAGGCAGCCA TGGTAGAATC AGTAGGCTTG 
GCCCTAGTAA AGCTACCAGA TGTCCTTAAT CGTTTGAAGC CTGATATCAT GATTGTTCAT 
GGAGACAGAT TTGATGCCCT GGCTCTGGCT ACATCTGCTG CCTTGATGAA TATCCGAATC 
CTTCACATTG AAGGTGGGGA AGTCAGTGGG ACCATTGATG ATTCTATCAG ACATGCCATC 
ACAAAACTGG CTCATTATCA TGTGTGCTGC ACCCGAAGTG CAGAGCAACA CCTGATATCC 
ATGTGTGAGG ACCATGATCG CATCCTTCTG GCAGGCTGCC CTTCCTATGA CAAACTTCTC 
TCTGCCAAGA ACAAAGACTA CATGAGCATC ATTCGGATGT GGTTAGGTGA TGATGTAAAA 
TCTAAAGATT ACATTGTTGC GCTACAACAC CCTGTGACCA CTGACATTAA GCATTCCATA 
AAAATGTTTG AATTAACATT GGATGCACTT ATCTCATTTA ACAAGCGGAC TCTAGTTCTG 
TTTCCAAACA TTGACGCAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAAGGCATC 
GAGCATCATC CCAATTTCCG TGCAGTTAAA CATGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCTCATG CAGGTTGTAT GATTGGGAAC AGCAGCTGTG GAGTACGAGA GGTTGGAGCT 
TTTGGAACAC CTGTGATCAA TCTAGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTCCTTCATG TCCGGGATGC TGATACCCAA GACAAAATAC TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AATACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCGAGGATT 
TTGAAGTTTC TCAAATCTAT TGATCTTCAA GAGCCACTAC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGCGCCCTG 
GCTGTTGATC TCGGTGGGAC AAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATT 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TAGAAGCTGC AGCAGAAGCT GTAAAACTCA ACTGCAGAAT TTTGGGAGTA 
GGTATTTCTA CGGGTGGCCG TGTAAATCCC CGGGAAGGAG TCGTGCTGCA TTCAACCAAA 
CTGATTCAAG AATGGAACTC CGTGGACCTC CGGACCCCGC TGTCTGACAC CTTGCATCTC 
CCCGTGTGGG TGGACAATGA TGGCAATTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGCC TGGAAAACTT TGTGACACTT ATCACAGGCA CAGGAATTGG CGGGGGGATC 
ATCCATCAAC ACGAGTTGAT CCATGGAAGC TCCTTCTGTG CTGCAGAACT TGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGACTGTTCC TGTGGAAGTC ATGGGTGTAT TGAAGCATAT 
GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA AAAAAGCTAC ATGATGAAGA CCTGCTCTTG 
GTGGAAGGGA TGTCCGTGCC AAAAGACGAG GCCGTGAGTG CAGTCCATCT CATCCAAGCT 
GCGAAACTTG GCAACGTGAA GGCCCAGAGC ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT 
CTTGGGGTTG TGAACATCCT CCACACTATA AATCCTTCCC TTGTGATCCT GTCTGGCGTC 
CTAGCCAGTC ACTACATCCA CATCGTCAAG GACGTCATCC GCCAGCAAGC CTTGTCTTCG 
GTTCAGGATG TGGATGTGGT GGTTTCAGAT TTGGTGGATC CTGCCCTGCT GGGTGCCGCC 
AGCATGGTTC TGGACTACAC AACTCGCAGG ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431623.1
CDS	103..2361
Translation	MEINVYPQWKLCLQGPHEFYFKNLSQKRRAMEKNGDNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVSAVHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTINPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIC

Target ORF information:

RefSeq Version	XM_003431575.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431575.3

ORF Insert Sequence:

ATGGAAATCA ATGTGTATCC TCAGTGGAAG TTGTGCCTTC AAGGACCTCA TGAATTCTAT 
TTTAAGAACC TCTCACAAAA GAGACGAGCC ATGGAGAAGA ATGGGGATAA CCGGAAGCTT 
CGAGTTTGTG TCGCCACTTG CAACCGTGCT GATTATTCCA AACTTGCCCC AATCATGTTT 
GGCATTAAAA TGGAACCTGA GTTCTTTGAA CTTGATGTGG TGGTACTTGG CTCTCACCTA 
ATAGATGATT ATGGGAACAC ATACCGCATG ATTGAACAAG ATGACTTTGA CATTAATACC 
AGGCTACACA CAATTGTGAG AGGGGAAGAT GAGGCAGCCA TGGTAGAATC AGTAGGCTTG 
GCCCTAGTAA AGCTACCAGA TGTCCTTAAT CGTTTGAAGC CTGATATCAT GATTGTTCAT 
GGAGACAGAT TTGATGCCCT GGCTCTGGCT ACATCTGCTG CCTTGATGAA TATCCGAATC 
CTTCACATTG AAGGTGGGGA AGTCAGTGGG ACCATTGATG ATTCTATCAG ACATGCCATC 
ACAAAACTGG CTCATTATCA TGTGTGCTGC ACCCGAAGTG CAGAGCAACA CCTGATATCC 
ATGTGTGAGG ACCATGATCG CATCCTTCTG GCAGGCTGCC CTTCCTATGA CAAACTTCTC 
TCTGCCAAGA ACAAAGACTA CATGAGCATC ATTCGGATGT GGTTAGGTGA TGATGTAAAA 
TCTAAAGATT ACATTGTTGC GCTACAACAC CCTGTGACCA CTGACATTAA GCATTCCATA 
AAAATGTTTG AATTAACATT GGATGCACTT ATCTCATTTA ACAAGCGGAC TCTAGTTCTG 
TTTCCAAACA TTGACGCAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAAGGCATC 
GAGCATCATC CCAATTTCCG TGCAGTTAAA CATGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCTCATG CAGGTTGTAT GATTGGGAAC AGCAGCTGTG GAGTACGAGA GGTTGGAGCT 
TTTGGAACAC CTGTGATCAA TCTAGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTCCTTCATG TCCGGGATGC TGATACCCAA GACAAAATAC TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AATACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCGAGGATT 
TTGAAGTTTC TCAAATCTAT TGATCTTCAA GAGCCACTAC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGCGCCCTG 
GCTGTTGATC TCGGTGGGAC AAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATT 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TAGAAGCTGC AGCAGAAGCT GTAAAACTCA ACTGCAGAAT TTTGGGAGTA 
GGTATTTCTA CGGGTGGCCG TGTAAATCCC CGGGAAGGAG TCGTGCTGCA TTCAACCAAA 
CTGATTCAAG AATGGAACTC CGTGGACCTC CGGACCCCGC TGTCTGACAC CTTGCATCTC 
CCCGTGTGGG TGGACAATGA TGGCAATTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGCC TGGAAAACTT TGTGACACTT ATCACAGGCA CAGGAATTGG CGGGGGGATC 
ATCCATCAAC ACGAGTTGAT CCATGGAAGC TCCTTCTGTG CTGCAGAACT TGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGACTGTTCC TGTGGAAGTC ATGGGTGTAT TGAAGCATAT 
GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA AAAAAGCTAC ATGATGAAGA CCTGCTCTTG 
GTGGAAGGGA TGTCCGTGCC AAAAGACGAG GCCGTGAGTG CAGTCCATCT CATCCAAGCT 
GCGAAACTTG GCAACGTGAA GGCCCAGAGC ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT 
CTTGGGGTTG TGAACATCCT CCACACTATA AATCCTTCCC TTGTGATCCT GTCTGGCGTC 
CTAGCCAGTC ACTACATCCA CATCGTCAAG GACGTCATCC GCCAGCAAGC CTTGTCTTCG 
GTTCAGGATG TGGATGTGGT GGTTTCAGAT TTGGTGGATC CTGCCCTGCT GGGTGCCGCC 
AGCATGGTTC TGGACTACAC AACTCGCAGG ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_003431575.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003431623.1 Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe14623
Clone ID Related Accession (Same CDS sequence)	XM_003431575.3 , XM_003431575.4
Accession Version	XM_003431575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003431575.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATCA ATGTGTATCC TCAGTGGAAG TTGTGCCTTC AAGGACCTCA TGAATTCTAT 
TTTAAGAACC TCTCACAAAA GAGACGAGCC ATGGAGAAGA ATGGGGATAA CCGGAAGCTT 
CGAGTTTGTG TCGCCACTTG CAACCGTGCT GATTATTCCA AACTTGCCCC AATCATGTTT 
GGCATTAAAA TGGAACCTGA GTTCTTTGAA CTTGATGTGG TGGTACTTGG CTCTCACCTA 
ATAGATGATT ATGGGAACAC ATACCGCATG ATTGAACAAG ATGACTTTGA CATTAATACC 
AGGCTACACA CAATTGTGAG AGGGGAAGAT GAGGCAGCCA TGGTAGAATC AGTAGGCTTG 
GCCCTAGTAA AGCTACCAGA TGTCCTTAAT CGTTTGAAGC CTGATATCAT GATTGTTCAT 
GGAGACAGAT TTGATGCCCT GGCTCTGGCT ACATCTGCTG CCTTGATGAA TATCCGAATC 
CTTCACATTG AAGGTGGGGA AGTCAGTGGG ACCATTGATG ATTCTATCAG ACATGCCATC 
ACAAAACTGG CTCATTATCA TGTGTGCTGC ACCCGAAGTG CAGAGCAACA CCTGATATCC 
ATGTGTGAGG ACCATGATCG CATCCTTCTG GCAGGCTGCC CTTCCTATGA CAAACTTCTC 
TCTGCCAAGA ACAAAGACTA CATGAGCATC ATTCGGATGT GGTTAGGTGA TGATGTAAAA 
TCTAAAGATT ACATTGTTGC GCTACAACAC CCTGTGACCA CTGACATTAA GCATTCCATA 
AAAATGTTTG AATTAACATT GGATGCACTT ATCTCATTTA ACAAGCGGAC TCTAGTTCTG 
TTTCCAAACA TTGACGCAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAAGGCATC 
GAGCATCATC CCAATTTCCG TGCAGTTAAA CATGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCTCATG CAGGTTGTAT GATTGGGAAC AGCAGCTGTG GAGTACGAGA GGTTGGAGCT 
TTTGGAACAC CTGTGATCAA TCTAGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTCCTTCATG TCCGGGATGC TGATACCCAA GACAAAATAC TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AATACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCGAGGATT 
TTGAAGTTTC TCAAATCTAT TGATCTTCAA GAGCCACTAC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGCGCCCTG 
GCTGTTGATC TCGGTGGGAC AAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATT 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TAGAAGCTGC AGCAGAAGCT GTAAAACTCA ACTGCAGAAT TTTGGGAGTA 
GGTATTTCTA CGGGTGGCCG TGTAAATCCC CGGGAAGGAG TCGTGCTGCA TTCAACCAAA 
CTGATTCAAG AATGGAACTC CGTGGACCTC CGGACCCCGC TGTCTGACAC CTTGCATCTC 
CCCGTGTGGG TGGACAATGA TGGCAATTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGCC TGGAAAACTT TGTGACACTT ATCACAGGCA CAGGAATTGG CGGGGGGATC 
ATCCATCAAC ACGAGTTGAT CCATGGAAGC TCCTTCTGTG CTGCAGAACT TGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGACTGTTCC TGTGGAAGTC ATGGGTGTAT TGAAGCATAT 
GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA AAAAAGCTAC ATGATGAAGA CCTGCTCTTG 
GTGGAAGGGA TGTCCGTGCC AAAAGACGAG GCCGTGAGTG CAGTCCATCT CATCCAAGCT 
GCGAAACTTG GCAACGTGAA GGCCCAGAGC ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT 
CTTGGGGTTG TGAACATCCT CCACACTATA AATCCTTCCC TTGTGATCCT GTCTGGCGTC 
CTAGCCAGTC ACTACATCCA CATCGTCAAG GACGTCATCC GCCAGCAAGC CTTGTCTTCG 
GTTCAGGATG TGGATGTGGT GGTTTCAGAT TTGGTGGATC CTGCCCTGCT GGGTGCCGCC 
AGCATGGTTC TGGACTACAC AACTCGCAGG ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431623.1
CDS	76..2334
Translation	MEINVYPQWKLCLQGPHEFYFKNLSQKRRAMEKNGDNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVSAVHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTINPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIC

Target ORF information:

RefSeq Version	XM_003431575.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431575.4

ORF Insert Sequence:

ATGGAAATCA ATGTGTATCC TCAGTGGAAG TTGTGCCTTC AAGGACCTCA TGAATTCTAT 
TTTAAGAACC TCTCACAAAA GAGACGAGCC ATGGAGAAGA ATGGGGATAA CCGGAAGCTT 
CGAGTTTGTG TCGCCACTTG CAACCGTGCT GATTATTCCA AACTTGCCCC AATCATGTTT 
GGCATTAAAA TGGAACCTGA GTTCTTTGAA CTTGATGTGG TGGTACTTGG CTCTCACCTA 
ATAGATGATT ATGGGAACAC ATACCGCATG ATTGAACAAG ATGACTTTGA CATTAATACC 
AGGCTACACA CAATTGTGAG AGGGGAAGAT GAGGCAGCCA TGGTAGAATC AGTAGGCTTG 
GCCCTAGTAA AGCTACCAGA TGTCCTTAAT CGTTTGAAGC CTGATATCAT GATTGTTCAT 
GGAGACAGAT TTGATGCCCT GGCTCTGGCT ACATCTGCTG CCTTGATGAA TATCCGAATC 
CTTCACATTG AAGGTGGGGA AGTCAGTGGG ACCATTGATG ATTCTATCAG ACATGCCATC 
ACAAAACTGG CTCATTATCA TGTGTGCTGC ACCCGAAGTG CAGAGCAACA CCTGATATCC 
ATGTGTGAGG ACCATGATCG CATCCTTCTG GCAGGCTGCC CTTCCTATGA CAAACTTCTC 
TCTGCCAAGA ACAAAGACTA CATGAGCATC ATTCGGATGT GGTTAGGTGA TGATGTAAAA 
TCTAAAGATT ACATTGTTGC GCTACAACAC CCTGTGACCA CTGACATTAA GCATTCCATA 
AAAATGTTTG AATTAACATT GGATGCACTT ATCTCATTTA ACAAGCGGAC TCTAGTTCTG 
TTTCCAAACA TTGACGCAGG GAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAAGGCATC 
GAGCATCATC CCAATTTCCG TGCAGTTAAA CATGTCCCAT TTGACCAGTT TATACAGTTG 
GTTGCTCATG CAGGTTGTAT GATTGGGAAC AGCAGCTGTG GAGTACGAGA GGTTGGAGCT 
TTTGGAACAC CTGTGATCAA TCTAGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTCCTTCATG TCCGGGATGC TGATACCCAA GACAAAATAC TGCAAGCACT GCACCTTCAG 
TTTGGTAAAC AATACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCGAGGATT 
TTGAAGTTTC TCAAATCTAT TGATCTTCAA GAGCCACTAC AAAAGAAATT CTGCTTTCCT 
CCTGTGAAGG AGAATATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGCGCCCTG 
GCTGTTGATC TCGGTGGGAC AAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATT 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACCTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TAGAAGCTGC AGCAGAAGCT GTAAAACTCA ACTGCAGAAT TTTGGGAGTA 
GGTATTTCTA CGGGTGGCCG TGTAAATCCC CGGGAAGGAG TCGTGCTGCA TTCAACCAAA 
CTGATTCAAG AATGGAACTC CGTGGACCTC CGGACCCCGC TGTCTGACAC CTTGCATCTC 
CCCGTGTGGG TGGACAATGA TGGCAATTGT GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA 
GGAAAGGGCC TGGAAAACTT TGTGACACTT ATCACAGGCA CAGGAATTGG CGGGGGGATC 
ATCCATCAAC ACGAGTTGAT CCATGGAAGC TCCTTCTGTG CTGCAGAACT TGGCCACCTT 
GTTGTGTCTC TGGATGGGCC TGACTGTTCC TGTGGAAGTC ATGGGTGTAT TGAAGCATAT 
GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA AAAAAGCTAC ATGATGAAGA CCTGCTCTTG 
GTGGAAGGGA TGTCCGTGCC AAAAGACGAG GCCGTGAGTG CAGTCCATCT CATCCAAGCT 
GCGAAACTTG GCAACGTGAA GGCCCAGAGC ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT 
CTTGGGGTTG TGAACATCCT CCACACTATA AATCCTTCCC TTGTGATCCT GTCTGGCGTC 
CTAGCCAGTC ACTACATCCA CATCGTCAAG GACGTCATCC GCCAGCAAGC CTTGTCTTCG 
GTTCAGGATG TGGATGTGGT GGTTTCAGAT TTGGTGGATC CTGCCCTGCT GGGTGCCGCC 
AGCATGGTTC TGGACTACAC AACTCGCAGG ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_005626809.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626866.1 Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe14625
Clone ID Related Accession (Same CDS sequence)	XM_005626809.3 , XM_005626809.2 , XM_005626808.2
Accession Version	XM_005626809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGTATTTCTA CGGGTGGCCG TGTAAATCCC 
CGGGAAGGAG TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
CGGACCCCGC TGTCTGACAC CTTGCATCTC CCCGTGTGGG TGGACAATGA TGGCAATTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGCC TGGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGAATTGG CGGGGGGATC ATCCATCAAC ACGAGTTGAT CCATGGAAGC 
TCCTTCTGTG CTGCAGAACT TGGCCACCTT GTTGTGTCTC TGGATGGGCC TGACTGTTCC 
TGTGGAAGTC ATGGGTGTAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA 
AAAAAGCTAC ATGATGAAGA CCTGCTCTTG GTGGAAGGGA TGTCCGTGCC AAAAGACGAG 
GCCGTGAGTG CAGTCCATCT CATCCAAGCT GCGAAACTTG GCAACGTGAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT CTTGGGGTTG TGAACATCCT CCACACTATA 
AATCCTTCCC TTGTGATCCT GTCTGGCGTC CTAGCCAGTC ACTACATCCA CATCGTCAAG 
GACGTCATCC GCCAGCAAGC CTTGTCTTCG GTTCAGGATG TGGATGTGGT GGTTTCAGAT 
TTGGTGGATC CTGCCCTGCT GGGTGCCGCC AGCATGGTTC TGGACTACAC AACTCGCAGG 
ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626866.1
CDS	198..2366
Translation	MEKNGDNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVSAVHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTINPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIC

Target ORF information:

RefSeq Version	XM_005626809.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626809.3

ORF Insert Sequence:

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGTATTTCTA CGGGTGGCCG TGTAAATCCC 
CGGGAAGGAG TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
CGGACCCCGC TGTCTGACAC CTTGCATCTC CCCGTGTGGG TGGACAATGA TGGCAATTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGCC TGGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGAATTGG CGGGGGGATC ATCCATCAAC ACGAGTTGAT CCATGGAAGC 
TCCTTCTGTG CTGCAGAACT TGGCCACCTT GTTGTGTCTC TGGATGGGCC TGACTGTTCC 
TGTGGAAGTC ATGGGTGTAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA 
AAAAAGCTAC ATGATGAAGA CCTGCTCTTG GTGGAAGGGA TGTCCGTGCC AAAAGACGAG 
GCCGTGAGTG CAGTCCATCT CATCCAAGCT GCGAAACTTG GCAACGTGAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT CTTGGGGTTG TGAACATCCT CCACACTATA 
AATCCTTCCC TTGTGATCCT GTCTGGCGTC CTAGCCAGTC ACTACATCCA CATCGTCAAG 
GACGTCATCC GCCAGCAAGC CTTGTCTTCG GTTCAGGATG TGGATGTGGT GGTTTCAGAT 
TTGGTGGATC CTGCCCTGCT GGGTGCCGCC AGCATGGTTC TGGACTACAC AACTCGCAGG 
ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_005626809.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626866.1 Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe14625
Clone ID Related Accession (Same CDS sequence)	XM_005626809.3 , XM_005626809.2 , XM_005626808.2
Accession Version	XM_005626809.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGTATTTCTA CGGGTGGCCG TGTAAATCCC 
CGGGAAGGAG TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
CGGACCCCGC TGTCTGACAC CTTGCATCTC CCCGTGTGGG TGGACAATGA TGGCAATTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGCC TGGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGAATTGG CGGGGGGATC ATCCATCAAC ACGAGTTGAT CCATGGAAGC 
TCCTTCTGTG CTGCAGAACT TGGCCACCTT GTTGTGTCTC TGGATGGGCC TGACTGTTCC 
TGTGGAAGTC ATGGGTGTAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA 
AAAAAGCTAC ATGATGAAGA CCTGCTCTTG GTGGAAGGGA TGTCCGTGCC AAAAGACGAG 
GCCGTGAGTG CAGTCCATCT CATCCAAGCT GCGAAACTTG GCAACGTGAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT CTTGGGGTTG TGAACATCCT CCACACTATA 
AATCCTTCCC TTGTGATCCT GTCTGGCGTC CTAGCCAGTC ACTACATCCA CATCGTCAAG 
GACGTCATCC GCCAGCAAGC CTTGTCTTCG GTTCAGGATG TGGATGTGGT GGTTTCAGAT 
TTGGTGGATC CTGCCCTGCT GGGTGCCGCC AGCATGGTTC TGGACTACAC AACTCGCAGG 
ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626866.1
CDS	217..2385
Translation	MEKNGDNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVSAVHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTINPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIC

Target ORF information:

RefSeq Version	XM_005626809.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626809.2

ORF Insert Sequence:

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGTATTTCTA CGGGTGGCCG TGTAAATCCC 
CGGGAAGGAG TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
CGGACCCCGC TGTCTGACAC CTTGCATCTC CCCGTGTGGG TGGACAATGA TGGCAATTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGCC TGGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGAATTGG CGGGGGGATC ATCCATCAAC ACGAGTTGAT CCATGGAAGC 
TCCTTCTGTG CTGCAGAACT TGGCCACCTT GTTGTGTCTC TGGATGGGCC TGACTGTTCC 
TGTGGAAGTC ATGGGTGTAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA 
AAAAAGCTAC ATGATGAAGA CCTGCTCTTG GTGGAAGGGA TGTCCGTGCC AAAAGACGAG 
GCCGTGAGTG CAGTCCATCT CATCCAAGCT GCGAAACTTG GCAACGTGAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT CTTGGGGTTG TGAACATCCT CCACACTATA 
AATCCTTCCC TTGTGATCCT GTCTGGCGTC CTAGCCAGTC ACTACATCCA CATCGTCAAG 
GACGTCATCC GCCAGCAAGC CTTGTCTTCG GTTCAGGATG TGGATGTGGT GGTTTCAGAT 
TTGGTGGATC CTGCCCTGCT GGGTGCCGCC AGCATGGTTC TGGACTACAC AACTCGCAGG 
ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_005626808.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005626865.1 Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe14625
Clone ID Related Accession (Same CDS sequence)	XM_005626809.3 , XM_005626809.2 , XM_005626808.2
Accession Version	XM_005626808.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGTATTTCTA CGGGTGGCCG TGTAAATCCC 
CGGGAAGGAG TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
CGGACCCCGC TGTCTGACAC CTTGCATCTC CCCGTGTGGG TGGACAATGA TGGCAATTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGCC TGGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGAATTGG CGGGGGGATC ATCCATCAAC ACGAGTTGAT CCATGGAAGC 
TCCTTCTGTG CTGCAGAACT TGGCCACCTT GTTGTGTCTC TGGATGGGCC TGACTGTTCC 
TGTGGAAGTC ATGGGTGTAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA 
AAAAAGCTAC ATGATGAAGA CCTGCTCTTG GTGGAAGGGA TGTCCGTGCC AAAAGACGAG 
GCCGTGAGTG CAGTCCATCT CATCCAAGCT GCGAAACTTG GCAACGTGAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT CTTGGGGTTG TGAACATCCT CCACACTATA 
AATCCTTCCC TTGTGATCCT GTCTGGCGTC CTAGCCAGTC ACTACATCCA CATCGTCAAG 
GACGTCATCC GCCAGCAAGC CTTGTCTTCG GTTCAGGATG TGGATGTGGT GGTTTCAGAT 
TTGGTGGATC CTGCCCTGCT GGGTGCCGCC AGCATGGTTC TGGACTACAC AACTCGCAGG 
ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005626865.1
CDS	435..2603
Translation	MEKNGDNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGVVLHSTKLIQEWNSVDLRTPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVSAVHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTINPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIC

Target ORF information:

RefSeq Version	XM_005626808.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005626808.2

ORF Insert Sequence:

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGTATTTCTA CGGGTGGCCG TGTAAATCCC 
CGGGAAGGAG TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
CGGACCCCGC TGTCTGACAC CTTGCATCTC CCCGTGTGGG TGGACAATGA TGGCAATTGT 
GCTGCCCTGG CGGAAAGGAA ATTTGGCCAA GGAAAGGGCC TGGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGAATTGG CGGGGGGATC ATCCATCAAC ACGAGTTGAT CCATGGAAGC 
TCCTTCTGTG CTGCAGAACT TGGCCACCTT GTTGTGTCTC TGGATGGGCC TGACTGTTCC 
TGTGGAAGTC ATGGGTGTAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGAGAAGCA 
AAAAAGCTAC ATGATGAAGA CCTGCTCTTG GTGGAAGGGA TGTCCGTGCC AAAAGACGAG 
GCCGTGAGTG CAGTCCATCT CATCCAAGCT GCGAAACTTG GCAACGTGAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTCTGGGT CTTGGGGTTG TGAACATCCT CCACACTATA 
AATCCTTCCC TTGTGATCCT GTCTGGCGTC CTAGCCAGTC ACTACATCCA CATCGTCAAG 
GACGTCATCC GCCAGCAAGC CTTGTCTTCG GTTCAGGATG TGGATGTGGT GGTTTCAGAT 
TTGGTGGATC CTGCCCTGCT GGGTGCCGCC AGCATGGTTC TGGACTACAC AACTCGCAGG 
ATCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_022425601.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022281309.1 Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

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CloneID	OCe292175
Clone ID Related Accession (Same CDS sequence)	XM_022425601.1
Accession Version	XM_022425601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGAATTGGCG GGGGGATCAT CCATCAACAC 
GAGTTGATCC ATGGAAGCTC CTTCTGTGCT GCAGAACTTG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ACTGTTCCTG TGGAAGTCAT GGGTGTATTG AAGCATATGC CTCTGGAATG 
GCCTTGCAGA GAGAAGCAAA AAAGCTACAT GATGAAGACC TGCTCTTGGT GGAAGGGATG 
TCCGTGCCAA AAGACGAGGC CGTGAGTGCA GTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AACGTGAAGG CCCAGAGCAT CTTGAGAACA GCTGGAACAG CTCTGGGTCT TGGGGTTGTG 
AACATCCTCC ACACTATAAA TCCTTCCCTT GTGATCCTGT CTGGCGTCCT AGCCAGTCAC 
TACATCCACA TCGTCAAGGA CGTCATCCGC CAGCAAGCCT TGTCTTCGGT TCAGGATGTG 
GATGTGGTGG TTTCAGATTT GGTGGATCCT GCCCTGCTGG GTGCCGCCAG CATGGTTCTG 
GACTACACAA CTCGCAGGAT CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022281309.1
CDS	62..2008
Translation	MEKNGDNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKSKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKENISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGIGGGIIHQHELIHGSSFCAAELGHLVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDLLLVEGMSVPKDEAVSAVHLIQAAKLGNVKAQSILRTAGTALGLGVVNILHTINPSLVILSGVLASHYIHIVKDVIRQQALSSVQDVDVVVSDLVDPALLGAASMVLDYTTRRIC

Target ORF information:

RefSeq Version	XM_022425601.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022425601.1

ORF Insert Sequence:

ATGGAGAAGA ATGGGGATAA CCGGAAGCTT CGAGTTTGTG TCGCCACTTG CAACCGTGCT 
GATTATTCCA AACTTGCCCC AATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGATGTGG TGGTACTTGG CTCTCACCTA ATAGATGATT ATGGGAACAC ATACCGCATG 
ATTGAACAAG ATGACTTTGA CATTAATACC AGGCTACACA CAATTGTGAG AGGGGAAGAT 
GAGGCAGCCA TGGTAGAATC AGTAGGCTTG GCCCTAGTAA AGCTACCAGA TGTCCTTAAT 
CGTTTGAAGC CTGATATCAT GATTGTTCAT GGAGACAGAT TTGATGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA TATCCGAATC CTTCACATTG AAGGTGGGGA AGTCAGTGGG 
ACCATTGATG ATTCTATCAG ACATGCCATC ACAAAACTGG CTCATTATCA TGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGATCG CATCCTTCTG 
GCAGGCTGCC CTTCCTATGA CAAACTTCTC TCTGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA TCTAAAGATT ACATTGTTGC GCTACAACAC 
CCTGTGACCA CTGACATTAA GCATTCCATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC TCTAGTTCTG TTTCCAAACA TTGACGCAGG GAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAAGGCATC GAGCATCATC CCAATTTCCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGTTG GTTGCTCATG CAGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GAGTACGAGA GGTTGGAGCT TTTGGAACAC CTGTGATCAA TCTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAC TGCAAGCACT GCACCTTCAG TTTGGTAAAC AATACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCGAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT CTGCTTTCCT CCTGTGAAGG AGAATATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGCGCCCTG GCTGTTGATC TCGGTGGGAC AAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATT GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACCTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCTGC AGCAGAAGCT 
GTAAAACTCA ACTGCAGAAT TTTGGGAGTA GGAATTGGCG GGGGGATCAT CCATCAACAC 
GAGTTGATCC ATGGAAGCTC CTTCTGTGCT GCAGAACTTG GCCACCTTGT TGTGTCTCTG 
GATGGGCCTG ACTGTTCCTG TGGAAGTCAT GGGTGTATTG AAGCATATGC CTCTGGAATG 
GCCTTGCAGA GAGAAGCAAA AAAGCTACAT GATGAAGACC TGCTCTTGGT GGAAGGGATG 
TCCGTGCCAA AAGACGAGGC CGTGAGTGCA GTCCATCTCA TCCAAGCTGC GAAACTTGGC 
AACGTGAAGG CCCAGAGCAT CTTGAGAACA GCTGGAACAG CTCTGGGTCT TGGGGTTGTG 
AACATCCTCC ACACTATAAA TCCTTCCCTT GTGATCCTGT CTGGCGTCCT AGCCAGTCAC 
TACATCCACA TCGTCAAGGA CGTCATCCGC CAGCAAGCCT TGTCTTCGGT TCAGGATGTG 
GATGTGGTGG TTTCAGATTT GGTGGATCCT GCCCTGCTGG GTGCCGCCAG CATGGTTCTG 
GACTACACAA CTCGCAGGAT CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GNE cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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