FOCAD cDNA ORF clone, Canis lupus familiaris(Dog)

The following FOCAD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FOCAD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe14448 XM_005626671.3
Latest version!
Canis lupus familiaris focadhesin (FOCAD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe14449 XM_538678.6
Latest version!
Canis lupus familiaris focadhesin (FOCAD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe14450 XM_005626673.2
Latest version!
Canis lupus familiaris focadhesin (FOCAD), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OCe14449 XM_538678.5 Canis lupus familiaris focadhesin (FOCAD), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe14448 XM_005626671.2 Canis lupus familiaris focadhesin (FOCAD), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe14448
    Clone ID Related Accession (Same CDS sequence) XM_005626671.2 , XM_005626671.3
    Accession Version XM_005626671.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product focadhesin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626671.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGCAGGTTC AGCCTAATTT CCTTTCGATG
    AAGGAGTGGG TTTCCATGGT GTCTGATACT CTCCTGATCA TTGTGGATAG CCATTACCAA
    CCCAGAGGAC AACTTTTCTC CTCATTTTAT TATAAATCCT ATTCTGGTGA AAACACAGCT
    AGTGCGATTG CCCGCTCTGC TGCCGCCACG GCTTTGTCTC TCCTCGTGCC AGTTTTCATT
    ATTTCTTGCA AAGAGAAGGT TGAGGAAATC CTAAACATGC TGACTGCCAG GTTACCTGGG
    AAACCAAGAG CTGATGAGTC TCAAGCCGTG CAAATCCACA TGGGCCTTGC TTTGGGGATG
    TTTCTCTCTC GCTTGTGTGA GGAGAAACTC AGTGATATAT CCGGCCAACA GATGAACCTT
    CTTCTGATGA AGTCTCTGGA TGCCCTGGAA AATTGCTGCT TTGATGCTAG TCTGGAATAT
    AACACGGGCT GTATATTGGG AGTTGGACTT GTTCTGTCCC TTATGAGCCA CAGCAGCCAA
    ACGGAGTCTC GAGTTCACGT GGCAGCATCA CTTCGGAAGC TCTCGACTTA TCTAGATGAC
    AGTGGGAGCC AAAGCAGAAC ATTTCAGGAG ATCCTGGCCT ATACCCTCAG CTGCGTATGT
    ACATCGGCAT TCAGTGCTGG GATCATTGAG GCTGTAGAGG CTGAAGAAAT TATGAACAAG
    CTTCGGATAT TGGTAGAAAA CAATCAGCAG ACTTCTGGTT TTGCCCTGGC GTTGGGAAAC
    CTAGTTCATG GCTTGTCTGT GTGTGGACAT GGGAAAGCTG AGGACTTGGG CCACAGACTG
    CTCCCCTCCT GGATAAGAAT TGTTCTAGCA CAGGGCACTC CTACAATGCT TTGTTTGGCA
    GCGCTTCATG GCATGGTGGC CTTGGTAGGC TCTGAAGGGG ATGTAATCCA GCTGAAGTCA
    GAAGCCATCC AATCCTCTCA GTTTCAAGCC AGACTTAATG AAGTCATTAA AACTGTAACT
    CAGGTCATCA GTGTGTCTGG AGTGATTGGT CTCCAATCAA ACGCTATCTG GCTTCTAGGA
    CATCTTCATC TATCCACTCT GTCCTCAAGT CAGAGTAGAA CCTCTGTTCC TACTGACTAT
    AGCTATTTGC CTGAGAGCAG TTTTATTAGA GCGGCCATTG GCTTCTTCGT TACAGGAGGA
    AAAAAAGGTC CTGAATCTGT GCTCCCGTCA CTTCTCAAAG TTGTAATGAA ACCCATCGCA
    GCTGTCGGAG AAAGCTACCA GTATCCTCCT GTGAACTGGG CTGCTCTCCT CTCTCCACTT
    ATGAGGCTAA ACTTTGGAGA AGAGATACAG CAACTGTGCC TTGAAATTAT GGTGACACAG
    GCCCAGTCAT CCCAGAATGC GGCTGCCCTT TTGGGACTGT GGGTGATGCC ACCACTGATC
    TATGGGCTGA GTTTGAATAT CAAGAAATAC CTGCTGGTGT CTGCACCTCT ATGGATAAAA
    CATGTCTCAG ACGAACAGAT CGTGGGTTTT GTTGAAAATG TGATGGTGGC AGTTTTTAAA
    GCAGCTTCCC CACTTCCCCA TCCTGAGTTA TGTGTGAGTG CCTTACAGGG TCTGAGTCAG
    GCCATGAAAC TGCCCAGCCC TACCCATCAC CTTTGGAGTC TGCTCTGTGA AGCTACTGGA
    AAAATTTTTG ACCTTCTGCC AAATAAGATT CGGAGAAATG ATCTAGAGGT ATATGTCAGT
    GTAGCAAAAT GCCTCTCAGA AATGACAGAT GATGAAGCCA ATCGGATTGC CCGGATTACT
    AAGAGCAGCA TAGAAAAGGC TGCCTTTGTC AGACTGTACT TGGTCTCTCA AGGACGATTC
    CCCTTGATGA GCTTGATGGA TATCCTCAGT GTTGCTGTTG AGCACAAGGA AAGAGACACA
    CTAGCTTGGA TGGTACTGCA CAGCCTCTAC CAGGCACGGA TTGTGAGCCA TGCTAACACA
    GGTGTGTTAA AGAGAATGGA ATGGCTCTTG GAACTGATGG GTTATATCAG AAATATTGCA
    TACCACTCGA CATCTATTCC GAACGTGGCT CTTGATGAGG CTTTGGACTT CTTGTTGCTG
    GTATTTGCAA CTGCAGTGGT TACGTGGGCG GACCACGGTG CCCCTCTCCT CCTTGGCCTC
    AGTGCTAGTT GGTTGCCATG GCATCAGGAA AATGGCCCAG CTGGGTCAGC TTCAAGCTTC
    CTTGGCAGGA GCCCAATGCA CAGGGTCGCT CTTCAGGAGG CTCTCACTCT CCTTCCCAGT
    AGCATGCCTC TTCTGCTGCA AAAAGAGCCA TGGAAGGAAC AGACCCAGAA GTTCATTGAC
    TGGCTCTTCA GCATCATGGA AAGCCCTAAA GAAGTCCTCT CAGCAAAATC CAGGGATCTT
    TTAAAAGCCA CCCTGTTGTC CTTAAGAGTC CTCCCAGAGT TTAAGAAGAA AGCTGTATGG
    ACCAGAGCAT ATGGTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626728.1
    CDS84..5504
    Translation

    Target ORF information:

    RefSeq Version XM_005626671.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris focadhesin (FOCAD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626671.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGCAGGTTC AGCCTAATTT CCTTTCGATG
    AAGGAGTGGG TTTCCATGGT GTCTGATACT CTCCTGATCA TTGTGGATAG CCATTACCAA
    CCCAGAGGAC AACTTTTCTC CTCATTTTAT TATAAATCCT ATTCTGGTGA AAACACAGCT
    AGTGCGATTG CCCGCTCTGC TGCCGCCACG GCTTTGTCTC TCCTCGTGCC AGTTTTCATT
    ATTTCTTGCA AAGAGAAGGT TGAGGAAATC CTAAACATGC TGACTGCCAG GTTACCTGGG
    AAACCAAGAG CTGATGAGTC TCAAGCCGTG CAAATCCACA TGGGCCTTGC TTTGGGGATG
    TTTCTCTCTC GCTTGTGTGA GGAGAAACTC AGTGATATAT CCGGCCAACA GATGAACCTT
    CTTCTGATGA AGTCTCTGGA TGCCCTGGAA AATTGCTGCT TTGATGCTAG TCTGGAATAT
    AACACGGGCT GTATATTGGG AGTTGGACTT GTTCTGTCCC TTATGAGCCA CAGCAGCCAA
    ACGGAGTCTC GAGTTCACGT GGCAGCATCA CTTCGGAAGC TCTCGACTTA TCTAGATGAC
    AGTGGGAGCC AAAGCAGAAC ATTTCAGGAG ATCCTGGCCT ATACCCTCAG CTGCGTATGT
    ACATCGGCAT TCAGTGCTGG GATCATTGAG GCTGTAGAGG CTGAAGAAAT TATGAACAAG
    CTTCGGATAT TGGTAGAAAA CAATCAGCAG ACTTCTGGTT TTGCCCTGGC GTTGGGAAAC
    CTAGTTCATG GCTTGTCTGT GTGTGGACAT GGGAAAGCTG AGGACTTGGG CCACAGACTG
    CTCCCCTCCT GGATAAGAAT TGTTCTAGCA CAGGGCACTC CTACAATGCT TTGTTTGGCA
    GCGCTTCATG GCATGGTGGC CTTGGTAGGC TCTGAAGGGG ATGTAATCCA GCTGAAGTCA
    GAAGCCATCC AATCCTCTCA GTTTCAAGCC AGACTTAATG AAGTCATTAA AACTGTAACT
    CAGGTCATCA GTGTGTCTGG AGTGATTGGT CTCCAATCAA ACGCTATCTG GCTTCTAGGA
    CATCTTCATC TATCCACTCT GTCCTCAAGT CAGAGTAGAA CCTCTGTTCC TACTGACTAT
    AGCTATTTGC CTGAGAGCAG TTTTATTAGA GCGGCCATTG GCTTCTTCGT TACAGGAGGA
    AAAAAAGGTC CTGAATCTGT GCTCCCGTCA CTTCTCAAAG TTGTAATGAA ACCCATCGCA
    GCTGTCGGAG AAAGCTACCA GTATCCTCCT GTGAACTGGG CTGCTCTCCT CTCTCCACTT
    ATGAGGCTAA ACTTTGGAGA AGAGATACAG CAACTGTGCC TTGAAATTAT GGTGACACAG
    GCCCAGTCAT CCCAGAATGC GGCTGCCCTT TTGGGACTGT GGGTGATGCC ACCACTGATC
    TATGGGCTGA GTTTGAATAT CAAGAAATAC CTGCTGGTGT CTGCACCTCT ATGGATAAAA
    CATGTCTCAG ACGAACAGAT CGTGGGTTTT GTTGAAAATG TGATGGTGGC AGTTTTTAAA
    GCAGCTTCCC CACTTCCCCA TCCTGAGTTA TGTGTGAGTG CCTTACAGGG TCTGAGTCAG
    GCCATGAAAC TGCCCAGCCC TACCCATCAC CTTTGGAGTC TGCTCTGTGA AGCTACTGGA
    AAAATTTTTG ACCTTCTGCC AAATAAGATT CGGAGAAATG ATCTAGAGGT ATATGTCAGT
    GTAGCAAAAT GCCTCTCAGA AATGACAGAT GATGAAGCCA ATCGGATTGC CCGGATTACT
    AAGAGCAGCA TAGAAAAGGC TGCCTTTGTC AGACTGTACT TGGTCTCTCA AGGACGATTC
    CCCTTGATGA GCTTGATGGA TATCCTCAGT GTTGCTGTTG AGCACAAGGA AAGAGACACA
    CTAGCTTGGA TGGTACTGCA CAGCCTCTAC CAGGCACGGA TTGTGAGCCA TGCTAACACA
    GGTGTGTTAA AGAGAATGGA ATGGCTCTTG GAACTGATGG GTTATATCAG AAATATTGCA
    TACCACTCGA CATCTATTCC GAACGTGGCT CTTGATGAGG CTTTGGACTT CTTGTTGCTG
    GTATTTGCAA CTGCAGTGGT TACGTGGGCG GACCACGGTG CCCCTCTCCT CCTTGGCCTC
    AGTGCTAGTT GGTTGCCATG GCATCAGGAA AATGGCCCAG CTGGGTCAGC TTCAAGCTTC
    CTTGGCAGGA GCCCAATGCA CAGGGTCGCT CTTCAGGAGG CTCTCACTCT CCTTCCCAGT
    AGCATGCCTC TTCTGCTGCA AAAAGAGCCA TGGAAGGAAC AGACCCAGAA GTTCATTGAC
    TGGCTCTTCA GCATCATGGA AAGCCCTAAA GAAGTCCTCT CAGCAAAATC CAGGGATCTT
    TTAAAAGCCA CCCTGTTGTC CTTAAGAGTC CTCCCAGAGT TTAAGAAGAA AGCTGTATGG
    ACCAGAGCAT ATGGTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14448
    Clone ID Related Accession (Same CDS sequence) XM_005626671.2 , XM_005626671.3
    Accession Version XM_005626671.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product focadhesin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005626671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGCAGGTTC AGCCTAATTT CCTTTCGATG
    AAGGAGTGGG TTTCCATGGT GTCTGATACT CTCCTGATCA TTGTGGATAG CCATTACCAA
    CCCAGAGGAC AACTTTTCTC CTCATTTTAT TATAAATCCT ATTCTGGTGA AAACACAGCT
    AGTGCGATTG CCCGCTCTGC TGCCGCCACG GCTTTGTCTC TCCTCGTGCC AGTTTTCATT
    ATTTCTTGCA AAGAGAAGGT TGAGGAAATC CTAAACATGC TGACTGCCAG GTTACCTGGG
    AAACCAAGAG CTGATGAGTC TCAAGCCGTG CAAATCCACA TGGGCCTTGC TTTGGGGATG
    TTTCTCTCTC GCTTGTGTGA GGAGAAACTC AGTGATATAT CCGGCCAACA GATGAACCTT
    CTTCTGATGA AGTCTCTGGA TGCCCTGGAA AATTGCTGCT TTGATGCTAG TCTGGAATAT
    AACACGGGCT GTATATTGGG AGTTGGACTT GTTCTGTCCC TTATGAGCCA CAGCAGCCAA
    ACGGAGTCTC GAGTTCACGT GGCAGCATCA CTTCGGAAGC TCTCGACTTA TCTAGATGAC
    AGTGGGAGCC AAAGCAGAAC ATTTCAGGAG ATCCTGGCCT ATACCCTCAG CTGCGTATGT
    ACATCGGCAT TCAGTGCTGG GATCATTGAG GCTGTAGAGG CTGAAGAAAT TATGAACAAG
    CTTCGGATAT TGGTAGAAAA CAATCAGCAG ACTTCTGGTT TTGCCCTGGC GTTGGGAAAC
    CTAGTTCATG GCTTGTCTGT GTGTGGACAT GGGAAAGCTG AGGACTTGGG CCACAGACTG
    CTCCCCTCCT GGATAAGAAT TGTTCTAGCA CAGGGCACTC CTACAATGCT TTGTTTGGCA
    GCGCTTCATG GCATGGTGGC CTTGGTAGGC TCTGAAGGGG ATGTAATCCA GCTGAAGTCA
    GAAGCCATCC AATCCTCTCA GTTTCAAGCC AGACTTAATG AAGTCATTAA AACTGTAACT
    CAGGTCATCA GTGTGTCTGG AGTGATTGGT CTCCAATCAA ACGCTATCTG GCTTCTAGGA
    CATCTTCATC TATCCACTCT GTCCTCAAGT CAGAGTAGAA CCTCTGTTCC TACTGACTAT
    AGCTATTTGC CTGAGAGCAG TTTTATTAGA GCGGCCATTG GCTTCTTCGT TACAGGAGGA
    AAAAAAGGTC CTGAATCTGT GCTCCCGTCA CTTCTCAAAG TTGTAATGAA ACCCATCGCA
    GCTGTCGGAG AAAGCTACCA GTATCCTCCT GTGAACTGGG CTGCTCTCCT CTCTCCACTT
    ATGAGGCTAA ACTTTGGAGA AGAGATACAG CAACTGTGCC TTGAAATTAT GGTGACACAG
    GCCCAGTCAT CCCAGAATGC GGCTGCCCTT TTGGGACTGT GGGTGATGCC ACCACTGATC
    TATGGGCTGA GTTTGAATAT CAAGAAATAC CTGCTGGTGT CTGCACCTCT ATGGATAAAA
    CATGTCTCAG ACGAACAGAT CGTGGGTTTT GTTGAAAATG TGATGGTGGC AGTTTTTAAA
    GCAGCTTCCC CACTTCCCCA TCCTGAGTTA TGTGTGAGTG CCTTACAGGG TCTGAGTCAG
    GCCATGAAAC TGCCCAGCCC TACCCATCAC CTTTGGAGTC TGCTCTGTGA AGCTACTGGA
    AAAATTTTTG ACCTTCTGCC AAATAAGATT CGGAGAAATG ATCTAGAGGT ATATGTCAGT
    GTAGCAAAAT GCCTCTCAGA AATGACAGAT GATGAAGCCA ATCGGATTGC CCGGATTACT
    AAGAGCAGCA TAGAAAAGGC TGCCTTTGTC AGACTGTACT TGGTCTCTCA AGGACGATTC
    CCCTTGATGA GCTTGATGGA TATCCTCAGT GTTGCTGTTG AGCACAAGGA AAGAGACACA
    CTAGCTTGGA TGGTACTGCA CAGCCTCTAC CAGGCACGGA TTGTGAGCCA TGCTAACACA
    GGTGTGTTAA AGAGAATGGA ATGGCTCTTG GAACTGATGG GTTATATCAG AAATATTGCA
    TACCACTCGA CATCTATTCC GAACGTGGCT CTTGATGAGG CTTTGGACTT CTTGTTGCTG
    GTATTTGCAA CTGCAGTGGT TACGTGGGCG GACCACGGTG CCCCTCTCCT CCTTGGCCTC
    AGTGCTAGTT GGTTGCCATG GCATCAGGAA AATGGCCCAG CTGGGTCAGC TTCAAGCTTC
    CTTGGCAGGA GCCCAATGCA CAGGGTCGCT CTTCAGGAGG CTCTCACTCT CCTTCCCAGT
    AGCATGCCTC TTCTGCTGCA AAAAGAGCCA TGGAAGGAAC AGACCCAGAA GTTCATTGAC
    TGGCTCTTCA GCATCATGGA AAGCCCTAAA GAAGTCCTCT CAGCAAAATC CAGGGATCTT
    TTAAAAGCCA CCCTGTTGTC CTTAAGAGTC CTCCCAGAGT TTAAGAAGAA AGCTGTATGG
    ACCAGAGCAT ATGGTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626728.1
    CDS100..5520
    Translation

    Target ORF information:

    RefSeq Version XM_005626671.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris focadhesin (FOCAD), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626671.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGCAGGTTC AGCCTAATTT CCTTTCGATG
    AAGGAGTGGG TTTCCATGGT GTCTGATACT CTCCTGATCA TTGTGGATAG CCATTACCAA
    CCCAGAGGAC AACTTTTCTC CTCATTTTAT TATAAATCCT ATTCTGGTGA AAACACAGCT
    AGTGCGATTG CCCGCTCTGC TGCCGCCACG GCTTTGTCTC TCCTCGTGCC AGTTTTCATT
    ATTTCTTGCA AAGAGAAGGT TGAGGAAATC CTAAACATGC TGACTGCCAG GTTACCTGGG
    AAACCAAGAG CTGATGAGTC TCAAGCCGTG CAAATCCACA TGGGCCTTGC TTTGGGGATG
    TTTCTCTCTC GCTTGTGTGA GGAGAAACTC AGTGATATAT CCGGCCAACA GATGAACCTT
    CTTCTGATGA AGTCTCTGGA TGCCCTGGAA AATTGCTGCT TTGATGCTAG TCTGGAATAT
    AACACGGGCT GTATATTGGG AGTTGGACTT GTTCTGTCCC TTATGAGCCA CAGCAGCCAA
    ACGGAGTCTC GAGTTCACGT GGCAGCATCA CTTCGGAAGC TCTCGACTTA TCTAGATGAC
    AGTGGGAGCC AAAGCAGAAC ATTTCAGGAG ATCCTGGCCT ATACCCTCAG CTGCGTATGT
    ACATCGGCAT TCAGTGCTGG GATCATTGAG GCTGTAGAGG CTGAAGAAAT TATGAACAAG
    CTTCGGATAT TGGTAGAAAA CAATCAGCAG ACTTCTGGTT TTGCCCTGGC GTTGGGAAAC
    CTAGTTCATG GCTTGTCTGT GTGTGGACAT GGGAAAGCTG AGGACTTGGG CCACAGACTG
    CTCCCCTCCT GGATAAGAAT TGTTCTAGCA CAGGGCACTC CTACAATGCT TTGTTTGGCA
    GCGCTTCATG GCATGGTGGC CTTGGTAGGC TCTGAAGGGG ATGTAATCCA GCTGAAGTCA
    GAAGCCATCC AATCCTCTCA GTTTCAAGCC AGACTTAATG AAGTCATTAA AACTGTAACT
    CAGGTCATCA GTGTGTCTGG AGTGATTGGT CTCCAATCAA ACGCTATCTG GCTTCTAGGA
    CATCTTCATC TATCCACTCT GTCCTCAAGT CAGAGTAGAA CCTCTGTTCC TACTGACTAT
    AGCTATTTGC CTGAGAGCAG TTTTATTAGA GCGGCCATTG GCTTCTTCGT TACAGGAGGA
    AAAAAAGGTC CTGAATCTGT GCTCCCGTCA CTTCTCAAAG TTGTAATGAA ACCCATCGCA
    GCTGTCGGAG AAAGCTACCA GTATCCTCCT GTGAACTGGG CTGCTCTCCT CTCTCCACTT
    ATGAGGCTAA ACTTTGGAGA AGAGATACAG CAACTGTGCC TTGAAATTAT GGTGACACAG
    GCCCAGTCAT CCCAGAATGC GGCTGCCCTT TTGGGACTGT GGGTGATGCC ACCACTGATC
    TATGGGCTGA GTTTGAATAT CAAGAAATAC CTGCTGGTGT CTGCACCTCT ATGGATAAAA
    CATGTCTCAG ACGAACAGAT CGTGGGTTTT GTTGAAAATG TGATGGTGGC AGTTTTTAAA
    GCAGCTTCCC CACTTCCCCA TCCTGAGTTA TGTGTGAGTG CCTTACAGGG TCTGAGTCAG
    GCCATGAAAC TGCCCAGCCC TACCCATCAC CTTTGGAGTC TGCTCTGTGA AGCTACTGGA
    AAAATTTTTG ACCTTCTGCC AAATAAGATT CGGAGAAATG ATCTAGAGGT ATATGTCAGT
    GTAGCAAAAT GCCTCTCAGA AATGACAGAT GATGAAGCCA ATCGGATTGC CCGGATTACT
    AAGAGCAGCA TAGAAAAGGC TGCCTTTGTC AGACTGTACT TGGTCTCTCA AGGACGATTC
    CCCTTGATGA GCTTGATGGA TATCCTCAGT GTTGCTGTTG AGCACAAGGA AAGAGACACA
    CTAGCTTGGA TGGTACTGCA CAGCCTCTAC CAGGCACGGA TTGTGAGCCA TGCTAACACA
    GGTGTGTTAA AGAGAATGGA ATGGCTCTTG GAACTGATGG GTTATATCAG AAATATTGCA
    TACCACTCGA CATCTATTCC GAACGTGGCT CTTGATGAGG CTTTGGACTT CTTGTTGCTG
    GTATTTGCAA CTGCAGTGGT TACGTGGGCG GACCACGGTG CCCCTCTCCT CCTTGGCCTC
    AGTGCTAGTT GGTTGCCATG GCATCAGGAA AATGGCCCAG CTGGGTCAGC TTCAAGCTTC
    CTTGGCAGGA GCCCAATGCA CAGGGTCGCT CTTCAGGAGG CTCTCACTCT CCTTCCCAGT
    AGCATGCCTC TTCTGCTGCA AAAAGAGCCA TGGAAGGAAC AGACCCAGAA GTTCATTGAC
    TGGCTCTTCA GCATCATGGA AAGCCCTAAA GAAGTCCTCT CAGCAAAATC CAGGGATCTT
    TTAAAAGCCA CCCTGTTGTC CTTAAGAGTC CTCCCAGAGT TTAAGAAGAA AGCTGTATGG
    ACCAGAGCAT ATGGTTGGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14449
    Clone ID Related Accession (Same CDS sequence) XM_538678.6 , XM_538678.5
    Accession Version XM_538678.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product focadhesin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538678.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGGTTCAGC CTAATTTCCT TTCGATGAAG
    GAGTGGGTTT CCATGGTGTC TGATACTCTC CTGATCATTG TGGATAGCCA TTACCAACCC
    AGAGGACAAC TTTTCTCCTC ATTTTATTAT AAATCCTATT CTGGTGAAAA CACAGCTAGT
    GCGATTGCCC GCTCTGCTGC CGCCACGGCT TTGTCTCTCC TCGTGCCAGT TTTCATTATT
    TCTTGCAAAG AGAAGGTTGA GGAAATCCTA AACATGCTGA CTGCCAGGTT ACCTGGGAAA
    CCAAGAGCTG ATGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTTGCTTT GGGGATGTTT
    CTCTCTCGCT TGTGTGAGGA GAAACTCAGT GATATATCCG GCCAACAGAT GAACCTTCTT
    CTGATGAAGT CTCTGGATGC CCTGGAAAAT TGCTGCTTTG ATGCTAGTCT GGAATATAAC
    ACGGGCTGTA TATTGGGAGT TGGACTTGTT CTGTCCCTTA TGAGCCACAG CAGCCAAACG
    GAGTCTCGAG TTCACGTGGC AGCATCACTT CGGAAGCTCT CGACTTATCT AGATGACAGT
    GGGAGCCAAA GCAGAACATT TCAGGAGATC CTGGCCTATA CCCTCAGCTG CGTATGTACA
    TCGGCATTCA GTGCTGGGAT CATTGAGGCT GTAGAGGCTG AAGAAATTAT GAACAAGCTT
    CGGATATTGG TAGAAAACAA TCAGCAGACT TCTGGTTTTG CCCTGGCGTT GGGAAACCTA
    GTTCATGGCT TGTCTGTGTG TGGACATGGG AAAGCTGAGG ACTTGGGCCA CAGACTGCTC
    CCCTCCTGGA TAAGAATTGT TCTAGCACAG GGCACTCCTA CAATGCTTTG TTTGGCAGCG
    CTTCATGGCA TGGTGGCCTT GGTAGGCTCT GAAGGGGATG TAATCCAGCT GAAGTCAGAA
    GCCATCCAAT CCTCTCAGTT TCAAGCCAGA CTTAATGAAG TCATTAAAAC TGTAACTCAG
    GTCATCAGTG TGTCTGGAGT GATTGGTCTC CAATCAAACG CTATCTGGCT TCTAGGACAT
    CTTCATCTAT CCACTCTGTC CTCAAGTCAG AGTAGAACCT CTGTTCCTAC TGACTATAGC
    TATTTGCCTG AGAGCAGTTT TATTAGAGCG GCCATTGGCT TCTTCGTTAC AGGAGGAAAA
    AAAGGTCCTG AATCTGTGCT CCCGTCACTT CTCAAAGTTG TAATGAAACC CATCGCAGCT
    GTCGGAGAAA GCTACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG
    AGGCTAAACT TTGGAGAAGA GATACAGCAA CTGTGCCTTG AAATTATGGT GACACAGGCC
    CAGTCATCCC AGAATGCGGC TGCCCTTTTG GGACTGTGGG TGATGCCACC ACTGATCTAT
    GGGCTGAGTT TGAATATCAA GAAATACCTG CTGGTGTCTG CACCTCTATG GATAAAACAT
    GTCTCAGACG AACAGATCGT GGGTTTTGTT GAAAATGTGA TGGTGGCAGT TTTTAAAGCA
    GCTTCCCCAC TTCCCCATCC TGAGTTATGT GTGAGTGCCT TACAGGGTCT GAGTCAGGCC
    ATGAAACTGC CCAGCCCTAC CCATCACCTT TGGAGTCTGC TCTGTGAAGC TACTGGAAAA
    ATTTTTGACC TTCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGGTATA TGTCAGTGTA
    GCAAAATGCC TCTCAGAAAT GACAGATGAT GAAGCCAATC GGATTGCCCG GATTACTAAG
    AGCAGCATAG AAAAGGCTGC CTTTGTCAGA CTGTACTTGG TCTCTCAAGG ACGATTCCCC
    TTGATGAGCT TGATGGATAT CCTCAGTGTT GCTGTTGAGC ACAAGGAAAG AGACACACTA
    GCTTGGATGG TACTGCACAG CCTCTACCAG GCACGGATTG TGAGCCATGC TAACACAGGT
    GTGTTAAAGA GAATGGAATG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TATTGCATAC
    CACTCGACAT CTATTCCGAA CGTGGCTCTT GATGAGGCTT TGGACTTCTT GTTGCTGGTA
    TTTGCAACTG CAGTGGTTAC GTGGGCGGAC CACGGTGCCC CTCTCCTCCT TGGCCTCAGT
    GCTAGTTGGT TGCCATGGCA TCAGGAAAAT GGCCCAGCTG GGTCAGCTTC AAGCTTCCTT
    GGCAGGAGCC CAATGCACAG GGTCGCTCTT CAGGAGGCTC TCACTCTCCT TCCCAGTAGC
    ATGCCTCTTC TGCTGCAAAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG
    CTCTTCAGCA TCATGGAAAG CCCTAAAGAA GTCCTCTCAG CAAAATCCAG GGATCTTTTA
    AAAGCCACCC TGTTGTCCTT AAGAGTCCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC
    AGAGCATATG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538678.2
    CDS100..5517
    Translation

    Target ORF information:

    RefSeq Version XM_538678.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris focadhesin (FOCAD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538678.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGGTTCAGC CTAATTTCCT TTCGATGAAG
    GAGTGGGTTT CCATGGTGTC TGATACTCTC CTGATCATTG TGGATAGCCA TTACCAACCC
    AGAGGACAAC TTTTCTCCTC ATTTTATTAT AAATCCTATT CTGGTGAAAA CACAGCTAGT
    GCGATTGCCC GCTCTGCTGC CGCCACGGCT TTGTCTCTCC TCGTGCCAGT TTTCATTATT
    TCTTGCAAAG AGAAGGTTGA GGAAATCCTA AACATGCTGA CTGCCAGGTT ACCTGGGAAA
    CCAAGAGCTG ATGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTTGCTTT GGGGATGTTT
    CTCTCTCGCT TGTGTGAGGA GAAACTCAGT GATATATCCG GCCAACAGAT GAACCTTCTT
    CTGATGAAGT CTCTGGATGC CCTGGAAAAT TGCTGCTTTG ATGCTAGTCT GGAATATAAC
    ACGGGCTGTA TATTGGGAGT TGGACTTGTT CTGTCCCTTA TGAGCCACAG CAGCCAAACG
    GAGTCTCGAG TTCACGTGGC AGCATCACTT CGGAAGCTCT CGACTTATCT AGATGACAGT
    GGGAGCCAAA GCAGAACATT TCAGGAGATC CTGGCCTATA CCCTCAGCTG CGTATGTACA
    TCGGCATTCA GTGCTGGGAT CATTGAGGCT GTAGAGGCTG AAGAAATTAT GAACAAGCTT
    CGGATATTGG TAGAAAACAA TCAGCAGACT TCTGGTTTTG CCCTGGCGTT GGGAAACCTA
    GTTCATGGCT TGTCTGTGTG TGGACATGGG AAAGCTGAGG ACTTGGGCCA CAGACTGCTC
    CCCTCCTGGA TAAGAATTGT TCTAGCACAG GGCACTCCTA CAATGCTTTG TTTGGCAGCG
    CTTCATGGCA TGGTGGCCTT GGTAGGCTCT GAAGGGGATG TAATCCAGCT GAAGTCAGAA
    GCCATCCAAT CCTCTCAGTT TCAAGCCAGA CTTAATGAAG TCATTAAAAC TGTAACTCAG
    GTCATCAGTG TGTCTGGAGT GATTGGTCTC CAATCAAACG CTATCTGGCT TCTAGGACAT
    CTTCATCTAT CCACTCTGTC CTCAAGTCAG AGTAGAACCT CTGTTCCTAC TGACTATAGC
    TATTTGCCTG AGAGCAGTTT TATTAGAGCG GCCATTGGCT TCTTCGTTAC AGGAGGAAAA
    AAAGGTCCTG AATCTGTGCT CCCGTCACTT CTCAAAGTTG TAATGAAACC CATCGCAGCT
    GTCGGAGAAA GCTACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG
    AGGCTAAACT TTGGAGAAGA GATACAGCAA CTGTGCCTTG AAATTATGGT GACACAGGCC
    CAGTCATCCC AGAATGCGGC TGCCCTTTTG GGACTGTGGG TGATGCCACC ACTGATCTAT
    GGGCTGAGTT TGAATATCAA GAAATACCTG CTGGTGTCTG CACCTCTATG GATAAAACAT
    GTCTCAGACG AACAGATCGT GGGTTTTGTT GAAAATGTGA TGGTGGCAGT TTTTAAAGCA
    GCTTCCCCAC TTCCCCATCC TGAGTTATGT GTGAGTGCCT TACAGGGTCT GAGTCAGGCC
    ATGAAACTGC CCAGCCCTAC CCATCACCTT TGGAGTCTGC TCTGTGAAGC TACTGGAAAA
    ATTTTTGACC TTCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGGTATA TGTCAGTGTA
    GCAAAATGCC TCTCAGAAAT GACAGATGAT GAAGCCAATC GGATTGCCCG GATTACTAAG
    AGCAGCATAG AAAAGGCTGC CTTTGTCAGA CTGTACTTGG TCTCTCAAGG ACGATTCCCC
    TTGATGAGCT TGATGGATAT CCTCAGTGTT GCTGTTGAGC ACAAGGAAAG AGACACACTA
    GCTTGGATGG TACTGCACAG CCTCTACCAG GCACGGATTG TGAGCCATGC TAACACAGGT
    GTGTTAAAGA GAATGGAATG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TATTGCATAC
    CACTCGACAT CTATTCCGAA CGTGGCTCTT GATGAGGCTT TGGACTTCTT GTTGCTGGTA
    TTTGCAACTG CAGTGGTTAC GTGGGCGGAC CACGGTGCCC CTCTCCTCCT TGGCCTCAGT
    GCTAGTTGGT TGCCATGGCA TCAGGAAAAT GGCCCAGCTG GGTCAGCTTC AAGCTTCCTT
    GGCAGGAGCC CAATGCACAG GGTCGCTCTT CAGGAGGCTC TCACTCTCCT TCCCAGTAGC
    ATGCCTCTTC TGCTGCAAAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG
    CTCTTCAGCA TCATGGAAAG CCCTAAAGAA GTCCTCTCAG CAAAATCCAG GGATCTTTTA
    AAAGCCACCC TGTTGTCCTT AAGAGTCCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC
    AGAGCATATG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14449
    Clone ID Related Accession (Same CDS sequence) XM_538678.6 , XM_538678.5
    Accession Version XM_538678.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product focadhesin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538678.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGGTTCAGC CTAATTTCCT TTCGATGAAG
    GAGTGGGTTT CCATGGTGTC TGATACTCTC CTGATCATTG TGGATAGCCA TTACCAACCC
    AGAGGACAAC TTTTCTCCTC ATTTTATTAT AAATCCTATT CTGGTGAAAA CACAGCTAGT
    GCGATTGCCC GCTCTGCTGC CGCCACGGCT TTGTCTCTCC TCGTGCCAGT TTTCATTATT
    TCTTGCAAAG AGAAGGTTGA GGAAATCCTA AACATGCTGA CTGCCAGGTT ACCTGGGAAA
    CCAAGAGCTG ATGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTTGCTTT GGGGATGTTT
    CTCTCTCGCT TGTGTGAGGA GAAACTCAGT GATATATCCG GCCAACAGAT GAACCTTCTT
    CTGATGAAGT CTCTGGATGC CCTGGAAAAT TGCTGCTTTG ATGCTAGTCT GGAATATAAC
    ACGGGCTGTA TATTGGGAGT TGGACTTGTT CTGTCCCTTA TGAGCCACAG CAGCCAAACG
    GAGTCTCGAG TTCACGTGGC AGCATCACTT CGGAAGCTCT CGACTTATCT AGATGACAGT
    GGGAGCCAAA GCAGAACATT TCAGGAGATC CTGGCCTATA CCCTCAGCTG CGTATGTACA
    TCGGCATTCA GTGCTGGGAT CATTGAGGCT GTAGAGGCTG AAGAAATTAT GAACAAGCTT
    CGGATATTGG TAGAAAACAA TCAGCAGACT TCTGGTTTTG CCCTGGCGTT GGGAAACCTA
    GTTCATGGCT TGTCTGTGTG TGGACATGGG AAAGCTGAGG ACTTGGGCCA CAGACTGCTC
    CCCTCCTGGA TAAGAATTGT TCTAGCACAG GGCACTCCTA CAATGCTTTG TTTGGCAGCG
    CTTCATGGCA TGGTGGCCTT GGTAGGCTCT GAAGGGGATG TAATCCAGCT GAAGTCAGAA
    GCCATCCAAT CCTCTCAGTT TCAAGCCAGA CTTAATGAAG TCATTAAAAC TGTAACTCAG
    GTCATCAGTG TGTCTGGAGT GATTGGTCTC CAATCAAACG CTATCTGGCT TCTAGGACAT
    CTTCATCTAT CCACTCTGTC CTCAAGTCAG AGTAGAACCT CTGTTCCTAC TGACTATAGC
    TATTTGCCTG AGAGCAGTTT TATTAGAGCG GCCATTGGCT TCTTCGTTAC AGGAGGAAAA
    AAAGGTCCTG AATCTGTGCT CCCGTCACTT CTCAAAGTTG TAATGAAACC CATCGCAGCT
    GTCGGAGAAA GCTACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG
    AGGCTAAACT TTGGAGAAGA GATACAGCAA CTGTGCCTTG AAATTATGGT GACACAGGCC
    CAGTCATCCC AGAATGCGGC TGCCCTTTTG GGACTGTGGG TGATGCCACC ACTGATCTAT
    GGGCTGAGTT TGAATATCAA GAAATACCTG CTGGTGTCTG CACCTCTATG GATAAAACAT
    GTCTCAGACG AACAGATCGT GGGTTTTGTT GAAAATGTGA TGGTGGCAGT TTTTAAAGCA
    GCTTCCCCAC TTCCCCATCC TGAGTTATGT GTGAGTGCCT TACAGGGTCT GAGTCAGGCC
    ATGAAACTGC CCAGCCCTAC CCATCACCTT TGGAGTCTGC TCTGTGAAGC TACTGGAAAA
    ATTTTTGACC TTCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGGTATA TGTCAGTGTA
    GCAAAATGCC TCTCAGAAAT GACAGATGAT GAAGCCAATC GGATTGCCCG GATTACTAAG
    AGCAGCATAG AAAAGGCTGC CTTTGTCAGA CTGTACTTGG TCTCTCAAGG ACGATTCCCC
    TTGATGAGCT TGATGGATAT CCTCAGTGTT GCTGTTGAGC ACAAGGAAAG AGACACACTA
    GCTTGGATGG TACTGCACAG CCTCTACCAG GCACGGATTG TGAGCCATGC TAACACAGGT
    GTGTTAAAGA GAATGGAATG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TATTGCATAC
    CACTCGACAT CTATTCCGAA CGTGGCTCTT GATGAGGCTT TGGACTTCTT GTTGCTGGTA
    TTTGCAACTG CAGTGGTTAC GTGGGCGGAC CACGGTGCCC CTCTCCTCCT TGGCCTCAGT
    GCTAGTTGGT TGCCATGGCA TCAGGAAAAT GGCCCAGCTG GGTCAGCTTC AAGCTTCCTT
    GGCAGGAGCC CAATGCACAG GGTCGCTCTT CAGGAGGCTC TCACTCTCCT TCCCAGTAGC
    ATGCCTCTTC TGCTGCAAAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG
    CTCTTCAGCA TCATGGAAAG CCCTAAAGAA GTCCTCTCAG CAAAATCCAG GGATCTTTTA
    AAAGCCACCC TGTTGTCCTT AAGAGTCCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC
    AGAGCATATG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538678.2
    CDS84..5501
    Translation

    Target ORF information:

    RefSeq Version XM_538678.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris focadhesin (FOCAD), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538678.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA ATCACAGGCT 
    GTGGGTCATC TTATTGCTGC AGTTCTAAAG GAAAATGGTT TTTCAGAAAA GATCCACCAA
    TCCACAAACC AGACTCCAGC TTTGAACTTG CTGTGGGAGA AATGTTGCAG TGATAACGTG
    GTGGTTCGAA CGGCCTGCTG TGAGGGTCTG GTGGCCCTTG TTGCTCAGGA TCATGCAGAG
    TTCAGCTACG TGCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACACATGGA
    TTGATAAAAG CCATTATGAA ATTACTACAA ATGCAAGCTG TTAAGGAAGG ACAATGTGGG
    GAGAAGAATA TTCGGAATTT ATATACCATT CGAAACCATC CTCAGCCTTT GATCACAATG
    CTTGAACACA GACCTGATTG CTGGCCAGTA CTTCTGCAGC AACTGACAGC TTTTTTCCAG
    CAATGCCCAA AAAGGTCAGA AGTTTCATGT ATCCAAATAA TGGCACCATT TCTGTGGTAT
    CTATATTGCG AACCATCCCA GTTACAGGAA TATGCTAAAC TCCGACTAAC CCTGCTAAAA
    GTCTTACTTC AACCCTGGGT TCCTAGTGAC ATGGGACAGC CATCCACATT GGAACAACAG
    ATACTTCAGC TGTGTTGTGA CATGGTTCCG TGTTTGCAGG TAAAAGATTT GATCCAGACA
    ACAGAAGTGA TGCTGTTTAT TGAGGAAGTC TATTTAAGCC TTCTGCGTCA TCCCATTTTC
    TGGAAAGTTC AGCTTACTCA GTTGACCCTT CAGCTATTGT GTGTCTGCGA AGTCAGCTTA
    AAGATAACTG GGGAATGCTC ATCTCTAATT CACCTGTTAG ACCACAGTGT TGAACTTCTG
    AAGGAGGATT TTCCTGTTGA ACTCGTCATA ATTGGAATAG CTTTACTATT GCTACAGACT
    CCAGCAAGTC AGCAGAAGCC AATCCTAAGT CTAGCTTTGA AGCTTCTCTC TCTTGCGGAG
    GGTCAGAAAA TCCCAAGGTC ATCCTTGGTG CTGGTGATGC CAGTTCTGCA GATACTGTCT
    TCTACTGCCT TGGAAGACTG TATATACATG GATGAAGAAG GTCCCTCAAG GCAGCAGTTG
    GCTCTAGACG TTTTGGAAAT GGTACAGCAG GAGTCCTACA GAAATGACCA CCAAAAGCTC
    TCCTACAAGC TTGTGTTCCC CATAACTGGC ACGTATGGAT CAATATTTAC AGCCTGGAGG
    ATCCTTGAAG TAATGAGAGA AGAGTCTGCT GCAAGTGACT GGTTGTTTTC CATAGAGTCC
    TTGCTCCCTA TTACCACGGC GATCCCTGTA CATGTTTTCC TTTTGCTGGC TCACCTCCTT
    GTTGAAGATC AAGGGCAAAA TCTTCGCCAA ATCCTGAAGG TCACTACAGA ATTAGCCCAG
    GCAGATTCCT CTCAGGTACC CAATCTGATT CCAGTTTTGA TGTTCAAGTT GGGAAGACCA
    CTGGAACCTG TATTATATAA TGACATCTTG TATACTTTAC CCTCACTTGG TGTCCATAAG
    GTGTGTATAG GACAAATTCT ACGAGTGATC CAACTTCTTG GAACCACTCC ACAACTAAGA
    GCTGTCACTC TACGCTTGTT GACATCTTTG TGGGAAAAGC AGGATCGTGT ATATCCTGAA
    CTGCAGCGTT TCATGGCCAT GTCTGATGTG CCCTCTCTGT CTGTGGGCAA GGAAGTCCAG
    TGGGAAAAAC TCATTGCCAA AGCAGCATCG ATCAGAGACA TATGTAAGCA GAGGCCCTAT
    CAGCATGGTG CAGATATGTT GGCAGCCGTT TCCCAGGTGT TGAATGAATG CACCAAACCT
    GATCAAGCTA CTCCCGCAGC CTTGGTCCTC CAAGGCCTCC ACGCGCTCTG CCAAGCGGAG
    GTCGTTTGCA TTCGCTCCAC ATGGAATGCT CTCTCTCCAA AGCTCAGCTG TGACACAAGA
    CCCCTCATTT TGAAGACCCT CAGTGAACTG TTTTCTCTGG TTCCCTCTTT AACAGTCAAT
    ATAGCTGAAT ATGAGAATTT TAAAGTTCAA GTCCTCAGCT TCCTCTGGAC CCACACTCAA
    AACAAGGACC CCATTGTAGC AAATGCTGCA TATAAATCTC TGTCACAATT CAGTGCAGGA
    GAGCACACCA TTCTTCATCT GCCTGAAGAG ATAAGACCAG AAATCCCGAC TCCTGATGAG
    CTAGATGAAG ACGAAGATGA TGAGGGCGAT GAAAAGGATC TGGATCTTTC CATTCCTGGC
    TCTTGCTATC TTAGACTGTT GTCACTTACT GCTCCTTTGG TTTTACCAGC TTTGGAAGAA
    TTTTTTACAT CTCTCGTGAA GCAAGAAATG GTGAATATGC CCCGTGGAAT CTATCACTCT
    GCATTAAAAG GAGGTGTCCG CTCAGACCAA GGAAAGACTG TAGCAGGAAT CCCCAATTTT
    ATATTGAAAA TGTATGAAAC CAATAAACAA CCAGGTTTGA AACCTGGCCT TGCTGGTGGT
    ATGTTATTTT GTTACGATAT TTCAACTTAT CAGAGTAAAG ATGGCAAACC ATTGCATAGA
    CTGATGGCCA GTAGAGGGCG AAGTTTCAAG CAGACCTCAC TTGCTCTAGT GCACGAGGTT
    CACATCCAGC CTTCCGAGTG GCACCGTGCA ATTTTCCTGC CACAGGCCTG GCTTGCATAT
    ATGAGTCGAG CTTATCATGC CATTTTACAG GGCAGGATGG GAGAGCTGGA CTTGCAACTG
    AAACATGGGA AAGAAGGACC TGAAGAGGTG CAGTACAAAA AAGGCACAGC CTGGCTCTGG
    GTCCGAGACA TGTTGACTGA GGAGATCACC AAGGCGGTGG CAAAGGAGAG CCCAGTGGTC
    AAGGGCAACG CACTGTTAGC CTTAAGCAGC CTCGCTGTCG TCGTATCCAA ACACGAAGCC
    AGCCTCTCCT CAGACTCTGA GGGGGTCCCA GAGGTTCAGC CTAATTTCCT TTCGATGAAG
    GAGTGGGTTT CCATGGTGTC TGATACTCTC CTGATCATTG TGGATAGCCA TTACCAACCC
    AGAGGACAAC TTTTCTCCTC ATTTTATTAT AAATCCTATT CTGGTGAAAA CACAGCTAGT
    GCGATTGCCC GCTCTGCTGC CGCCACGGCT TTGTCTCTCC TCGTGCCAGT TTTCATTATT
    TCTTGCAAAG AGAAGGTTGA GGAAATCCTA AACATGCTGA CTGCCAGGTT ACCTGGGAAA
    CCAAGAGCTG ATGAGTCTCA AGCCGTGCAA ATCCACATGG GCCTTGCTTT GGGGATGTTT
    CTCTCTCGCT TGTGTGAGGA GAAACTCAGT GATATATCCG GCCAACAGAT GAACCTTCTT
    CTGATGAAGT CTCTGGATGC CCTGGAAAAT TGCTGCTTTG ATGCTAGTCT GGAATATAAC
    ACGGGCTGTA TATTGGGAGT TGGACTTGTT CTGTCCCTTA TGAGCCACAG CAGCCAAACG
    GAGTCTCGAG TTCACGTGGC AGCATCACTT CGGAAGCTCT CGACTTATCT AGATGACAGT
    GGGAGCCAAA GCAGAACATT TCAGGAGATC CTGGCCTATA CCCTCAGCTG CGTATGTACA
    TCGGCATTCA GTGCTGGGAT CATTGAGGCT GTAGAGGCTG AAGAAATTAT GAACAAGCTT
    CGGATATTGG TAGAAAACAA TCAGCAGACT TCTGGTTTTG CCCTGGCGTT GGGAAACCTA
    GTTCATGGCT TGTCTGTGTG TGGACATGGG AAAGCTGAGG ACTTGGGCCA CAGACTGCTC
    CCCTCCTGGA TAAGAATTGT TCTAGCACAG GGCACTCCTA CAATGCTTTG TTTGGCAGCG
    CTTCATGGCA TGGTGGCCTT GGTAGGCTCT GAAGGGGATG TAATCCAGCT GAAGTCAGAA
    GCCATCCAAT CCTCTCAGTT TCAAGCCAGA CTTAATGAAG TCATTAAAAC TGTAACTCAG
    GTCATCAGTG TGTCTGGAGT GATTGGTCTC CAATCAAACG CTATCTGGCT TCTAGGACAT
    CTTCATCTAT CCACTCTGTC CTCAAGTCAG AGTAGAACCT CTGTTCCTAC TGACTATAGC
    TATTTGCCTG AGAGCAGTTT TATTAGAGCG GCCATTGGCT TCTTCGTTAC AGGAGGAAAA
    AAAGGTCCTG AATCTGTGCT CCCGTCACTT CTCAAAGTTG TAATGAAACC CATCGCAGCT
    GTCGGAGAAA GCTACCAGTA TCCTCCTGTG AACTGGGCTG CTCTCCTCTC TCCACTTATG
    AGGCTAAACT TTGGAGAAGA GATACAGCAA CTGTGCCTTG AAATTATGGT GACACAGGCC
    CAGTCATCCC AGAATGCGGC TGCCCTTTTG GGACTGTGGG TGATGCCACC ACTGATCTAT
    GGGCTGAGTT TGAATATCAA GAAATACCTG CTGGTGTCTG CACCTCTATG GATAAAACAT
    GTCTCAGACG AACAGATCGT GGGTTTTGTT GAAAATGTGA TGGTGGCAGT TTTTAAAGCA
    GCTTCCCCAC TTCCCCATCC TGAGTTATGT GTGAGTGCCT TACAGGGTCT GAGTCAGGCC
    ATGAAACTGC CCAGCCCTAC CCATCACCTT TGGAGTCTGC TCTGTGAAGC TACTGGAAAA
    ATTTTTGACC TTCTGCCAAA TAAGATTCGG AGAAATGATC TAGAGGTATA TGTCAGTGTA
    GCAAAATGCC TCTCAGAAAT GACAGATGAT GAAGCCAATC GGATTGCCCG GATTACTAAG
    AGCAGCATAG AAAAGGCTGC CTTTGTCAGA CTGTACTTGG TCTCTCAAGG ACGATTCCCC
    TTGATGAGCT TGATGGATAT CCTCAGTGTT GCTGTTGAGC ACAAGGAAAG AGACACACTA
    GCTTGGATGG TACTGCACAG CCTCTACCAG GCACGGATTG TGAGCCATGC TAACACAGGT
    GTGTTAAAGA GAATGGAATG GCTCTTGGAA CTGATGGGTT ATATCAGAAA TATTGCATAC
    CACTCGACAT CTATTCCGAA CGTGGCTCTT GATGAGGCTT TGGACTTCTT GTTGCTGGTA
    TTTGCAACTG CAGTGGTTAC GTGGGCGGAC CACGGTGCCC CTCTCCTCCT TGGCCTCAGT
    GCTAGTTGGT TGCCATGGCA TCAGGAAAAT GGCCCAGCTG GGTCAGCTTC AAGCTTCCTT
    GGCAGGAGCC CAATGCACAG GGTCGCTCTT CAGGAGGCTC TCACTCTCCT TCCCAGTAGC
    ATGCCTCTTC TGCTGCAAAA AGAGCCATGG AAGGAACAGA CCCAGAAGTT CATTGACTGG
    CTCTTCAGCA TCATGGAAAG CCCTAAAGAA GTCCTCTCAG CAAAATCCAG GGATCTTTTA
    AAAGCCACCC TGTTGTCCTT AAGAGTCCTC CCAGAGTTTA AGAAGAAAGC TGTATGGACC
    AGAGCATATG GTTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14450
    Clone ID Related Accession (Same CDS sequence) XM_005626673.2
    Accession Version XM_005626673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5175bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product focadhesin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGGTTTC TCCAAGATTT AAGGGGCGCA GACATAGCTT GGAATACACA TGGATTGATA 
    AAAGCCATTA TGAAATTACT ACAAATGCAA GCTGTTAAGG AAGGACAATG TGGGGAGAAG
    AATATTCGGA ATTTATATAC CATTCGAAAC CATCCTCAGC CTTTGATCAC AATGCTTGAA
    CACAGACCTG ATTGCTGGCC AGTACTTCTG CAGCAACTGA CAGCTTTTTT CCAGCAATGC
    CCAAAAAGGT CAGAAGTTTC ATGTATCCAA ATAATGGCAC CATTTCTGTG GTATCTATAT
    TGCGAACCAT CCCAGTTACA GGAATATGCT AAACTCCGAC TAACCCTGCT AAAAGTCTTA
    CTTCAACCCT GGGTTCCTAG TGACATGGGA CAGCCATCCA CATTGGAACA ACAGATACTT
    CAGCTGTGTT GTGACATGGT TCCGTGTTTG CAGGTAAAAG ATTTGATCCA GACAACAGAA
    GTGATGCTGT TTATTGAGGA AGTCTATTTA AGCCTTCTGC GTCATCCCAT TTTCTGGAAA
    GTTCAGCTTA CTCAGTTGAC CCTTCAGCTA TTGTGTGTCT GCGAAGTCAG CTTAAAGATA
    ACTGGGGAAT GCTCATCTCT AATTCACCTG TTAGACCACA GTGTTGAACT TCTGAAGGAG
    GATTTTCCTG TTGAACTCGT CATAATTGGA ATAGCTTTAC TATTGCTACA GACTCCAGCA
    AGTCAGCAGA AGCCAATCCT AAGTCTAGCT TTGAAGCTTC TCTCTCTTGC GGAGGGTCAG
    AAAATCCCAA GGTCATCCTT GGTGCTGGTG ATGCCAGTTC TGCAGATACT GTCTTCTACT
    GCCTTGGAAG ACTGTATATA CATGGATGAA GAAGGTCCCT CAAGGCAGCA GTTGGCTCTA
    GACGTTTTGG AAATGGTACA GCAGGAGTCC TACAGAAATG ACCACCAAAA GCTCTCCTAC
    AAGCTTGTGT TCCCCATAAC TGGCACGTAT GGATCAATAT TTACAGCCTG GAGGATCCTT
    GAAGTAATGA GAGAAGAGTC TGCTGCAAGT GACTGGTTGT TTTCCATAGA GTCCTTGCTC
    CCTATTACCA CGGCGATCCC TGTACATGTT TTCCTTTTGC TGGCTCACCT CCTTGTTGAA
    GATCAAGGGC AAAATCTTCG CCAAATCCTG AAGGTCACTA CAGAATTAGC CCAGGCAGAT
    TCCTCTCAGG TACCCAATCT GATTCCAGTT TTGATGTTCA AGTTGGGAAG ACCACTGGAA
    CCTGTATTAT ATAATGACAT CTTGTATACT TTACCCTCAC TTGGTGTCCA TAAGGTGTGT
    ATAGGACAAA TTCTACGAGT GATCCAACTT CTTGGAACCA CTCCACAACT AAGAGCTGTC
    ACTCTACGCT TGTTGACATC TTTGTGGGAA AAGCAGGATC GTGTATATCC TGAACTGCAG
    CGTTTCATGG CCATGTCTGA TGTGCCCTCT CTGTCTGTGG GCAAGGAAGT CCAGTGGGAA
    AAACTCATTG CCAAAGCAGC ATCGATCAGA GACATATGTA AGCAGAGGCC CTATCAGCAT
    GGTGCAGATA TGTTGGCAGC CGTTTCCCAG GTGTTGAATG AATGCACCAA ACCTGATCAA
    GCTACTCCCG CAGCCTTGGT CCTCCAAGGC CTCCACGCGC TCTGCCAAGC GGAGGTCGTT
    TGCATTCGCT CCACATGGAA TGCTCTCTCT CCAAAGCTCA GCTGTGACAC AAGACCCCTC
    ATTTTGAAGA CCCTCAGTGA ACTGTTTTCT CTGGTTCCCT CTTTAACAGT CAATATAGCT
    GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACCCACAC TCAAAACAAG
    GACCCCATTG TAGCAAATGC TGCATATAAA TCTCTGTCAC AATTCAGTGC AGGAGAGCAC
    ACCATTCTTC ATCTGCCTGA AGAGATAAGA CCAGAAATCC CGACTCCTGA TGAGCTAGAT
    GAAGACGAAG ATGATGAGGG CGATGAAAAG GATCTGGATC TTTCCATTCC TGGCTCTTGC
    TATCTTAGAC TGTTGTCACT TACTGCTCCT TTGGTTTTAC CAGCTTTGGA AGAATTTTTT
    ACATCTCTCG TGAAGCAAGA AATGGTGAAT ATGCCCCGTG GAATCTATCA CTCTGCATTA
    AAAGGAGGTG TCCGCTCAGA CCAAGGAAAG ACTGTAGCAG GAATCCCCAA TTTTATATTG
    AAAATGTATG AAACCAATAA ACAACCAGGT TTGAAACCTG GCCTTGCTGG TGGTATGTTA
    TTTTGTTACG ATATTTCAAC TTATCAGAGT AAAGATGGCA AACCATTGCA TAGACTGATG
    GCCAGTAGAG GGCGAAGTTT CAAGCAGACC TCACTTGCTC TAGTGCACGA GGTTCACATC
    CAGCCTTCCG AGTGGCACCG TGCAATTTTC CTGCCACAGG CCTGGCTTGC ATATATGAGT
    CGAGCTTATC ATGCCATTTT ACAGGGCAGG ATGGGAGAGC TGGACTTGCA ACTGAAACAT
    GGGAAAGAAG GACCTGAAGA GGTGCAGTAC AAAAAAGGCA CAGCCTGGCT CTGGGTCCGA
    GACATGTTGA CTGAGGAGAT CACCAAGGCG GTGGCAAAGG AGAGCCCAGT GGTCAAGGGC
    AACGCACTGT TAGCCTTAAG CAGCCTCGCT GTCGTCGTAT CCAAACACGA AGCCAGCCTC
    TCCTCAGACT CTGAGGGGGT CCCAGAGCAG GTTCAGCCTA ATTTCCTTTC GATGAAGGAG
    TGGGTTTCCA TGGTGTCTGA TACTCTCCTG ATCATTGTGG ATAGCCATTA CCAACCCAGA
    GGACAACTTT TCTCCTCATT TTATTATAAA TCCTATTCTG GTGAAAACAC AGCTAGTGCG
    ATTGCCCGCT CTGCTGCCGC CACGGCTTTG TCTCTCCTCG TGCCAGTTTT CATTATTTCT
    TGCAAAGAGA AGGTTGAGGA AATCCTAAAC ATGCTGACTG CCAGGTTACC TGGGAAACCA
    AGAGCTGATG AGTCTCAAGC CGTGCAAATC CACATGGGCC TTGCTTTGGG GATGTTTCTC
    TCTCGCTTGT GTGAGGAGAA ACTCAGTGAT ATATCCGGCC AACAGATGAA CCTTCTTCTG
    ATGAAGTCTC TGGATGCCCT GGAAAATTGC TGCTTTGATG CTAGTCTGGA ATATAACACG
    GGCTGTATAT TGGGAGTTGG ACTTGTTCTG TCCCTTATGA GCCACAGCAG CCAAACGGAG
    TCTCGAGTTC ACGTGGCAGC ATCACTTCGG AAGCTCTCGA CTTATCTAGA TGACAGTGGG
    AGCCAAAGCA GAACATTTCA GGAGATCCTG GCCTATACCC TCAGCTGCGT ATGTACATCG
    GCATTCAGTG CTGGGATCAT TGAGGCTGTA GAGGCTGAAG AAATTATGAA CAAGCTTCGG
    ATATTGGTAG AAAACAATCA GCAGACTTCT GGTTTTGCCC TGGCGTTGGG AAACCTAGTT
    CATGGCTTGT CTGTGTGTGG ACATGGGAAA GCTGAGGACT TGGGCCACAG ACTGCTCCCC
    TCCTGGATAA GAATTGTTCT AGCACAGGGC ACTCCTACAA TGCTTTGTTT GGCAGCGCTT
    CATGGCATGG TGGCCTTGGT AGGCTCTGAA GGGGATGTAA TCCAGCTGAA GTCAGAAGCC
    ATCCAATCCT CTCAGTTTCA AGCCAGACTT AATGAAGTCA TTAAAACTGT AACTCAGGTC
    ATCAGTGTGT CTGGAGTGAT TGGTCTCCAA TCAAACGCTA TCTGGCTTCT AGGACATCTT
    CATCTATCCA CTCTGTCCTC AAGTCAGAGT AGAACCTCTG TTCCTACTGA CTATAGCTAT
    TTGCCTGAGA GCAGTTTTAT TAGAGCGGCC ATTGGCTTCT TCGTTACAGG AGGAAAAAAA
    GGTCCTGAAT CTGTGCTCCC GTCACTTCTC AAAGTTGTAA TGAAACCCAT CGCAGCTGTC
    GGAGAAAGCT ACCAGTATCC TCCTGTGAAC TGGGCTGCTC TCCTCTCTCC ACTTATGAGG
    CTAAACTTTG GAGAAGAGAT ACAGCAACTG TGCCTTGAAA TTATGGTGAC ACAGGCCCAG
    TCATCCCAGA ATGCGGCTGC CCTTTTGGGA CTGTGGGTGA TGCCACCACT GATCTATGGG
    CTGAGTTTGA ATATCAAGAA ATACCTGCTG GTGTCTGCAC CTCTATGGAT AAAACATGTC
    TCAGACGAAC AGATCGTGGG TTTTGTTGAA AATGTGATGG TGGCAGTTTT TAAAGCAGCT
    TCCCCACTTC CCCATCCTGA GTTATGTGTG AGTGCCTTAC AGGGTCTGAG TCAGGCCATG
    AAACTGCCCA GCCCTACCCA TCACCTTTGG AGTCTGCTCT GTGAAGCTAC TGGAAAAATT
    TTTGACCTTC TGCCAAATAA GATTCGGAGA AATGATCTAG AGGTATATGT CAGTGTAGCA
    AAATGCCTCT CAGAAATGAC AGATGATGAA GCCAATCGGA TTGCCCGGAT TACTAAGAGC
    AGCATAGAAA AGGCTGCCTT TGTCAGACTG TACTTGGTCT CTCAAGGACG ATTCCCCTTG
    ATGAGCTTGA TGGATATCCT CAGTGTTGCT GTTGAGCACA AGGAAAGAGA CACACTAGCT
    TGGATGGTAC TGCACAGCCT CTACCAGGCA CGGATTGTGA GCCATGCTAA CACAGGTGTG
    TTAAAGAGAA TGGAATGGCT CTTGGAACTG ATGGGTTATA TCAGAAATAT TGCATACCAC
    TCGACATCTA TTCCGAACGT GGCTCTTGAT GAGGCTTTGG ACTTCTTGTT GCTGGTATTT
    GCAACTGCAG TGGTTACGTG GGCGGACCAC GGTGCCCCTC TCCTCCTTGG CCTCAGTGCT
    AGTTGGTTGC CATGGCATCA GGAAAATGGC CCAGCTGGGT CAGCTTCAAG CTTCCTTGGC
    AGGAGCCCAA TGCACAGGGT CGCTCTTCAG GAGGCTCTCA CTCTCCTTCC CAGTAGCATG
    CCTCTTCTGC TGCAAAAAGA GCCATGGAAG GAACAGACCC AGAAGTTCAT TGACTGGCTC
    TTCAGCATCA TGGAAAGCCC TAAAGAAGTC CTCTCAGCAA AATCCAGGGA TCTTTTAAAA
    GCCACCCTGT TGTCCTTAAG AGTCCTCCCA GAGTTTAAGA AGAAAGCTGT ATGGACCAGA
    GCATATGGTT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626730.1
    CDS27..5201
    Translation

    Target ORF information:

    RefSeq Version XM_005626673.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris focadhesin (FOCAD), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAGGTTTC TCCAAGATTT AAGGGGCGCA GACATAGCTT GGAATACACA TGGATTGATA 
    AAAGCCATTA TGAAATTACT ACAAATGCAA GCTGTTAAGG AAGGACAATG TGGGGAGAAG
    AATATTCGGA ATTTATATAC CATTCGAAAC CATCCTCAGC CTTTGATCAC AATGCTTGAA
    CACAGACCTG ATTGCTGGCC AGTACTTCTG CAGCAACTGA CAGCTTTTTT CCAGCAATGC
    CCAAAAAGGT CAGAAGTTTC ATGTATCCAA ATAATGGCAC CATTTCTGTG GTATCTATAT
    TGCGAACCAT CCCAGTTACA GGAATATGCT AAACTCCGAC TAACCCTGCT AAAAGTCTTA
    CTTCAACCCT GGGTTCCTAG TGACATGGGA CAGCCATCCA CATTGGAACA ACAGATACTT
    CAGCTGTGTT GTGACATGGT TCCGTGTTTG CAGGTAAAAG ATTTGATCCA GACAACAGAA
    GTGATGCTGT TTATTGAGGA AGTCTATTTA AGCCTTCTGC GTCATCCCAT TTTCTGGAAA
    GTTCAGCTTA CTCAGTTGAC CCTTCAGCTA TTGTGTGTCT GCGAAGTCAG CTTAAAGATA
    ACTGGGGAAT GCTCATCTCT AATTCACCTG TTAGACCACA GTGTTGAACT TCTGAAGGAG
    GATTTTCCTG TTGAACTCGT CATAATTGGA ATAGCTTTAC TATTGCTACA GACTCCAGCA
    AGTCAGCAGA AGCCAATCCT AAGTCTAGCT TTGAAGCTTC TCTCTCTTGC GGAGGGTCAG
    AAAATCCCAA GGTCATCCTT GGTGCTGGTG ATGCCAGTTC TGCAGATACT GTCTTCTACT
    GCCTTGGAAG ACTGTATATA CATGGATGAA GAAGGTCCCT CAAGGCAGCA GTTGGCTCTA
    GACGTTTTGG AAATGGTACA GCAGGAGTCC TACAGAAATG ACCACCAAAA GCTCTCCTAC
    AAGCTTGTGT TCCCCATAAC TGGCACGTAT GGATCAATAT TTACAGCCTG GAGGATCCTT
    GAAGTAATGA GAGAAGAGTC TGCTGCAAGT GACTGGTTGT TTTCCATAGA GTCCTTGCTC
    CCTATTACCA CGGCGATCCC TGTACATGTT TTCCTTTTGC TGGCTCACCT CCTTGTTGAA
    GATCAAGGGC AAAATCTTCG CCAAATCCTG AAGGTCACTA CAGAATTAGC CCAGGCAGAT
    TCCTCTCAGG TACCCAATCT GATTCCAGTT TTGATGTTCA AGTTGGGAAG ACCACTGGAA
    CCTGTATTAT ATAATGACAT CTTGTATACT TTACCCTCAC TTGGTGTCCA TAAGGTGTGT
    ATAGGACAAA TTCTACGAGT GATCCAACTT CTTGGAACCA CTCCACAACT AAGAGCTGTC
    ACTCTACGCT TGTTGACATC TTTGTGGGAA AAGCAGGATC GTGTATATCC TGAACTGCAG
    CGTTTCATGG CCATGTCTGA TGTGCCCTCT CTGTCTGTGG GCAAGGAAGT CCAGTGGGAA
    AAACTCATTG CCAAAGCAGC ATCGATCAGA GACATATGTA AGCAGAGGCC CTATCAGCAT
    GGTGCAGATA TGTTGGCAGC CGTTTCCCAG GTGTTGAATG AATGCACCAA ACCTGATCAA
    GCTACTCCCG CAGCCTTGGT CCTCCAAGGC CTCCACGCGC TCTGCCAAGC GGAGGTCGTT
    TGCATTCGCT CCACATGGAA TGCTCTCTCT CCAAAGCTCA GCTGTGACAC AAGACCCCTC
    ATTTTGAAGA CCCTCAGTGA ACTGTTTTCT CTGGTTCCCT CTTTAACAGT CAATATAGCT
    GAATATGAGA ATTTTAAAGT TCAAGTCCTC AGCTTCCTCT GGACCCACAC TCAAAACAAG
    GACCCCATTG TAGCAAATGC TGCATATAAA TCTCTGTCAC AATTCAGTGC AGGAGAGCAC
    ACCATTCTTC ATCTGCCTGA AGAGATAAGA CCAGAAATCC CGACTCCTGA TGAGCTAGAT
    GAAGACGAAG ATGATGAGGG CGATGAAAAG GATCTGGATC TTTCCATTCC TGGCTCTTGC
    TATCTTAGAC TGTTGTCACT TACTGCTCCT TTGGTTTTAC CAGCTTTGGA AGAATTTTTT
    ACATCTCTCG TGAAGCAAGA AATGGTGAAT ATGCCCCGTG GAATCTATCA CTCTGCATTA
    AAAGGAGGTG TCCGCTCAGA CCAAGGAAAG ACTGTAGCAG GAATCCCCAA TTTTATATTG
    AAAATGTATG AAACCAATAA ACAACCAGGT TTGAAACCTG GCCTTGCTGG TGGTATGTTA
    TTTTGTTACG ATATTTCAAC TTATCAGAGT AAAGATGGCA AACCATTGCA TAGACTGATG
    GCCAGTAGAG GGCGAAGTTT CAAGCAGACC TCACTTGCTC TAGTGCACGA GGTTCACATC
    CAGCCTTCCG AGTGGCACCG TGCAATTTTC CTGCCACAGG CCTGGCTTGC ATATATGAGT
    CGAGCTTATC ATGCCATTTT ACAGGGCAGG ATGGGAGAGC TGGACTTGCA ACTGAAACAT
    GGGAAAGAAG GACCTGAAGA GGTGCAGTAC AAAAAAGGCA CAGCCTGGCT CTGGGTCCGA
    GACATGTTGA CTGAGGAGAT CACCAAGGCG GTGGCAAAGG AGAGCCCAGT GGTCAAGGGC
    AACGCACTGT TAGCCTTAAG CAGCCTCGCT GTCGTCGTAT CCAAACACGA AGCCAGCCTC
    TCCTCAGACT CTGAGGGGGT CCCAGAGCAG GTTCAGCCTA ATTTCCTTTC GATGAAGGAG
    TGGGTTTCCA TGGTGTCTGA TACTCTCCTG ATCATTGTGG ATAGCCATTA CCAACCCAGA
    GGACAACTTT TCTCCTCATT TTATTATAAA TCCTATTCTG GTGAAAACAC AGCTAGTGCG
    ATTGCCCGCT CTGCTGCCGC CACGGCTTTG TCTCTCCTCG TGCCAGTTTT CATTATTTCT
    TGCAAAGAGA AGGTTGAGGA AATCCTAAAC ATGCTGACTG CCAGGTTACC TGGGAAACCA
    AGAGCTGATG AGTCTCAAGC CGTGCAAATC CACATGGGCC TTGCTTTGGG GATGTTTCTC
    TCTCGCTTGT GTGAGGAGAA ACTCAGTGAT ATATCCGGCC AACAGATGAA CCTTCTTCTG
    ATGAAGTCTC TGGATGCCCT GGAAAATTGC TGCTTTGATG CTAGTCTGGA ATATAACACG
    GGCTGTATAT TGGGAGTTGG ACTTGTTCTG TCCCTTATGA GCCACAGCAG CCAAACGGAG
    TCTCGAGTTC ACGTGGCAGC ATCACTTCGG AAGCTCTCGA CTTATCTAGA TGACAGTGGG
    AGCCAAAGCA GAACATTTCA GGAGATCCTG GCCTATACCC TCAGCTGCGT ATGTACATCG
    GCATTCAGTG CTGGGATCAT TGAGGCTGTA GAGGCTGAAG AAATTATGAA CAAGCTTCGG
    ATATTGGTAG AAAACAATCA GCAGACTTCT GGTTTTGCCC TGGCGTTGGG AAACCTAGTT
    CATGGCTTGT CTGTGTGTGG ACATGGGAAA GCTGAGGACT TGGGCCACAG ACTGCTCCCC
    TCCTGGATAA GAATTGTTCT AGCACAGGGC ACTCCTACAA TGCTTTGTTT GGCAGCGCTT
    CATGGCATGG TGGCCTTGGT AGGCTCTGAA GGGGATGTAA TCCAGCTGAA GTCAGAAGCC
    ATCCAATCCT CTCAGTTTCA AGCCAGACTT AATGAAGTCA TTAAAACTGT AACTCAGGTC
    ATCAGTGTGT CTGGAGTGAT TGGTCTCCAA TCAAACGCTA TCTGGCTTCT AGGACATCTT
    CATCTATCCA CTCTGTCCTC AAGTCAGAGT AGAACCTCTG TTCCTACTGA CTATAGCTAT
    TTGCCTGAGA GCAGTTTTAT TAGAGCGGCC ATTGGCTTCT TCGTTACAGG AGGAAAAAAA
    GGTCCTGAAT CTGTGCTCCC GTCACTTCTC AAAGTTGTAA TGAAACCCAT CGCAGCTGTC
    GGAGAAAGCT ACCAGTATCC TCCTGTGAAC TGGGCTGCTC TCCTCTCTCC ACTTATGAGG
    CTAAACTTTG GAGAAGAGAT ACAGCAACTG TGCCTTGAAA TTATGGTGAC ACAGGCCCAG
    TCATCCCAGA ATGCGGCTGC CCTTTTGGGA CTGTGGGTGA TGCCACCACT GATCTATGGG
    CTGAGTTTGA ATATCAAGAA ATACCTGCTG GTGTCTGCAC CTCTATGGAT AAAACATGTC
    TCAGACGAAC AGATCGTGGG TTTTGTTGAA AATGTGATGG TGGCAGTTTT TAAAGCAGCT
    TCCCCACTTC CCCATCCTGA GTTATGTGTG AGTGCCTTAC AGGGTCTGAG TCAGGCCATG
    AAACTGCCCA GCCCTACCCA TCACCTTTGG AGTCTGCTCT GTGAAGCTAC TGGAAAAATT
    TTTGACCTTC TGCCAAATAA GATTCGGAGA AATGATCTAG AGGTATATGT CAGTGTAGCA
    AAATGCCTCT CAGAAATGAC AGATGATGAA GCCAATCGGA TTGCCCGGAT TACTAAGAGC
    AGCATAGAAA AGGCTGCCTT TGTCAGACTG TACTTGGTCT CTCAAGGACG ATTCCCCTTG
    ATGAGCTTGA TGGATATCCT CAGTGTTGCT GTTGAGCACA AGGAAAGAGA CACACTAGCT
    TGGATGGTAC TGCACAGCCT CTACCAGGCA CGGATTGTGA GCCATGCTAA CACAGGTGTG
    TTAAAGAGAA TGGAATGGCT CTTGGAACTG ATGGGTTATA TCAGAAATAT TGCATACCAC
    TCGACATCTA TTCCGAACGT GGCTCTTGAT GAGGCTTTGG ACTTCTTGTT GCTGGTATTT
    GCAACTGCAG TGGTTACGTG GGCGGACCAC GGTGCCCCTC TCCTCCTTGG CCTCAGTGCT
    AGTTGGTTGC CATGGCATCA GGAAAATGGC CCAGCTGGGT CAGCTTCAAG CTTCCTTGGC
    AGGAGCCCAA TGCACAGGGT CGCTCTTCAG GAGGCTCTCA CTCTCCTTCC CAGTAGCATG
    CCTCTTCTGC TGCAAAAAGA GCCATGGAAG GAACAGACCC AGAAGTTCAT TGACTGGCTC
    TTCAGCATCA TGGAAAGCCC TAAAGAAGTC CTCTCAGCAA AATCCAGGGA TCTTTTAAAA
    GCCACCCTGT TGTCCTTAAG AGTCCTCCCA GAGTTTAAGA AGAAAGCTGT ATGGACCAGA
    GCATATGGTT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.