FREM1 cDNA ORF clone, Canis lupus familiaris(dog)

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe292067 XM_022425437.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe39155 XM_014117772.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe39154 XM_014117771.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe39154 XM_022425435.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OCe14408 XM_005626638.2
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_014117770.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_022425434.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_005626639.2
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_022425433.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_014117769.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_538668.4
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14408 XM_005626636.2
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14409 XM_022425436.1
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OCe14409 XM_005626640.2
Latest version!
Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe292067
    Clone ID Related Accession (Same CDS sequence) XM_022425437.1
    Accession Version XM_022425437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGTGTTTGC ATTGAAACGG AATCATCATT GTCCAGGAAC AAACGTGGTC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281145.1
    CDS888..6353
    Translation

    Target ORF information:

    RefSeq Version XM_022425437.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGTGTTTGC ATTGAAACGG AATCATCATT GTCCAGGAAC AAACGTGGTC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39155
    Clone ID Related Accession (Same CDS sequence) XM_014117772.1
    Accession Version XM_014117772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5367bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GAATGTCAAC TAAGATGTGG AATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973247.1
    CDS1045..6411
    Translation

    Target ORF information:

    RefSeq Version XM_014117772.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GAATGTCAAC TAAGATGTGG AATATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39154
    Clone ID Related Accession (Same CDS sequence) XM_022425435.1 , XM_014117771.1
    Accession Version XM_022425435.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GCGGCAAAGG ATTTCTCTTC ACTGTGGCTG ACCTTCAGGC TGGAGTTGTT
    CGCTATCATC ATGATGATAG TGACTCCACC AAAGATTTTG TGATCTTCAG GATATTCGAT
    GGCCATCACA GCATCCGCCA TAAGTTTCCC ATCAACATTT TGCCCAAAGA TGACAGTCCC
    CCATTTCTCA TAAGCAATGT TGTGATTGAA CTGGAGGAAG GGCAGACCAT ACTGATCCAG
    AGTTCTATGC TGAGAGCATC AGACATGGAC TCCAGTGATG ACTACATCTA TTTTAATATC
    ACGAAACCTC CTCAGGCTGG AGAGATCATG AAGAAGCCAG GGCCAGGACT GATAGGCTAT
    CCTGTCCCTG GCTTCCTTCA GAGGGATCTA TTTAATGGCA TCATTTACTA TCGTCACTTT
    GGTGGAGAGG TCTTTGAAGA CTCTTTTGAA TTTGTCCTGT GGGACAGCCA TGAACCTCCA
    AATTTCTCAG TGCCACAGGT GGTGACCATC CATATCACTC CAGTGGATGA CCAACTTCCA
    AAGGAGGCTC CTGGAGTTTC TCGGCATTTG GTTGTCAAGG AAACTGAGGT GGCCTACATA
    ACTAAGAAAC ATCTACATTT TATAGATACA GAATATAACA GGGAGCTTCT CTACACGATA
    ACGACACCTC CGTTTTTCTC TTTCGGCCAC AGACATCTGG ATGCTGGGAA ATTAATTATG
    GTGGATAGCA TTTCAAAGCT AACTAAAAAT TCTGCAGCCC GAGGGCTGAG ATCATTCACT
    CAGCATGCTG TAAACCACAT GAAAGTGGCC TACAAGCCAC CCATGCAAGA TATGGGCCTC
    AATCCAGGGT ATGTTCAGTT TGCATTTTCC ATCAGTAACC AGCATGGAGG CACTTTGCAT
    GGGATCTGCT TTAACATCAC AATTCTTCCA GTGGACAATC AAGTTCCTAA GGTGTTTACC
    AACCCCCTAA GAGTGGCTGA GGGAGCTCAA TGTGTCATCA GCACAGAAAA TGTTCTGATT
    TCTGATGTGG ATACCAAACT GGACAGCATC TACCTCTCCC TACAAAGACT GCCTCAGTGT
    GGAAGAGTGG AGCTGGATGG ATTTCCTCTA AACATAGGAG ACATATTTTC TTGGGGAGAC
    CTCCATGCCT TAAAAGTTAG GTATCAGCAT GATGGATCTG AGATACTTCA GGATGACATA
    TTCTTGGAGG TCACAGATGG CACGAATTCA GCAGAATTTA TTCTACACAT TGAGGTATTC
    CCTGTAAATG ATGAGCTACC AATCCTAAAG ACTGACCTCA TTCCCATGAT GCATTGCTCA
    GAGGGCGAAG AGGTAGTCAT CACCTCTGAA TACATTTTTG CTACTGATGT GGACAGTGAT
    GACTTGAAAC TGATGTTTGT GATTGCTCAA AAGCCTCAAC ATGGGGTGGT GAGGAAGGCT
    GGAGTCACAG TGGATCAGTT CTCTCAGGGA GATGTCATCT CGGGGGCTGT GACGTACAAA
    CACACGGGTG GTGAGATTGG CCTGGTGCCT TGTTTTGATA CCATTACATT GGTGGTTTCG
    GATGAAGAAG CTGGCCCTTT TGTGAATGGC TGTTGCTACA ATGGGCCTCA TCCATCTATT
    CCTCTTCATG AGTCCTTTCC AGTGTATGCC CTCAACATCA CAGTACATCC AGTGGACAAC
    CAGTCACCTT CAATCGCAAT AGGTACCATG TTTGTGGTAG ATGAGGGTTT CTCCGCAGCC
    GTTACCATTA ACCATTTGTC CGCCACTGAC CCTGACACTG CAACAGATGA CTTGGAATTT
    GTTTTGGTTT CTCCTCCCCA GTTTGGCTAC CTTGAAAACA CACTTCCTTC TGCAGGTTTT
    GAAAAAAGCA ATATGGGCAT AAGCATAGCT TCGTTCCCGT GGAGAGACAT GAAGGCTTTG
    CACATTAACT ATGTGCAGGC TAGGCATCTA AGGATGGAAC CGACGGCTGA CCAGTTCATG
    GTGTATGTCA CAGATGGGAA GCAACGCTCC TTGGAGATAC CATTTTACAT TATAATCAAC
    CCCATGAATG ATGAAGCTCC TGACTTTGTA GTGCAGAATA TTACTGTGTG TGAGGGTCAG
    ATGAAAGAAC TGGAATCCTC CACCATCAAT GCAGCTGACC TGGATGTTCC CAAAGACCCC
    TTGCTGTTCA GCATCACTCA TAAACCACGC CATGGCCTCC TCATCAACAG GGTGCTTAGC
    AAAGACTTTC CTCAAAATAA GCAGTCTGTC AACCCTGGCC AGAAGCACGA ACTTGTTCAG
    AACTTTTCCT TGGAACTTCT CAAGAAAGGA ATGAAGTTGA TGTATGTGCA TGATGACTCA
    GAGACTCTTG CTGATGACTT TACAATCCAG TTGTCAGATG GGAAACATAA GATATTAAAA
    ACCATTTCAG TAGAGATCAC CCCAGTTAAT GATGAGAAAC CAATGCTGAG CAAGAAGGCT
    GAAATTACAA TGAAAATGGG TGAAACTCGA ATTATTTCTA GTGCTGTTCT TTCAGCCATA
    GATAAAGACT CACCCAGGGA GAAGATTTAC TATTTATTTG AAAGGCTTCC ACAAAATGGG
    CAACTTCAAC TTAAGACAGG GAGGGACTGG GTCCCTCTCT ACCCTGGCAT GAAGTGCACT
    CAGGAAGAAG TGGATTTGAA CTTGCTGAGA TACACACACA CCGGGGCAAT GGATTCCCAG
    GACCAAGACA GCTTTACCTT CTACCTCTGG GATGGTGACA ACAGATCACC GGCATTTGAC
    TGTCACATCA CCATCAAGGA TATGGGAAAA GGTGATATTG TCATCCTTGT GAAACCGCTG
    GTGGTGTCTA AAGGTGATCG AGGTTTCTTG ACGACCACCA CTCTCCTTGC TGTGGATGGA
    ACAGACAAAC CTGAGGAACT ACTCTATGTC ATCACCTCCC CTCCGCGGTA TGGCCAGGTC
    CAGTATGTCC GCTATCCTGG AGTTCCCATT ACAAGCTTCA GCCAAATGGA CATAGCAGGA
    CAGACAGTCT GCTATGTACA TAAGAGTAAG GCAGCTGTCT CCCGTGACAC CTTCAGATTC
    ATCGTCAGCA ATGGCCTGCA GACCAAACGT GGGGTGTTTG AGATCATACT GGAGACCATG
    GACAGAGCCT TACCCGTGGT GACCAGGAAC AGGGGGCTGA GACTGGACGA AGGGACCACG
    GGCCTGCTCT CCCCTGACAT CCTCCAGCTG ACCGACCCTG ACACCCCAGC GGAGAACCTC
    ACCTTCATGT TGGCCCAGCT CCCACAACAC GGCCAGCTTT ACCTGCGGGG GACAGTGCTG
    CTTCAACACA ACTTCACCCA GCAGGACGTG GACAGCAGGA ATGTGGCCTA TAGGCACTTG
    GGAGGAGACT CCCAGGTGGA CTGCTTTACG TTTGTGGCCA CCGATAGTAC GAACCAAGGC
    TTCGTTGTGG ATGGGAGAGT GGGACAAGAA CCTGTATCAT TCACCATCCA GGTTGACCAG
    TTGGACAAAA CAGCCCCCCA CATCACACAT TTGCATTCCC CTTCTCAAGT GGTGCTCCTG
    AAAAATGGCT GTTACGGGAT TTACCTCACT TCCCATGTGC TGAAGGCATC GGACCCTGAC
    ACCAATGACG ATCTGATCAT CTTTAAGATT TTACGAGGCC CCCAACATGG ACATCTGGAG
    AACACAACCA CAGGTGAATT TATCCATGAG AAATTTGTCC AAAAGGACTT AAACAGTAAG
    ACCATTCTTT ACGTCATAAA CCCATCTTTG GATGTGAATT CAGATATCAT GGAATTCCAA
    ATCATGGACC CCACAGGGAA CTCTGCTACT CCTCAAATTT TGGAACTGAA ATGGTCTCAT
    ATTGAGTGGT CACAGACGGA ATTTGAGGTC TGCGAGAGTA TGGGCATGTT GCCCTTGGAA
    ATTACCAGAA GGGGATATTC CATGGACTCA GCCTTCGTGA GTGTAAAGGT CAACCAGGTG
    TCAGCTACAG TTGGAAAAGA CTTCACTCTG ACTCCATCTA AACTGATTCA GTTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGACGAT
    GAGGCCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCAG TTCTTGGCAC CAAGACAAAA
    GCTGCAGTGA AAATTTTGGA CTCAAAAGGA GGACAGTGTC ATCCTTCATA TTCCTTCAAC
    CCAAACAAGC ACAATGCAAG GGAGAAGGGC ATTTGGCACC CGCTGTCCCC AGGGTCTTCC
    TCATCCACCA CTTCTGGTTC CTTTCATCTG GAAAGAAGAC CCCTTCCATC TTCCAAGCGG
    CCAGCAGTCA CTAGGGGAGA CACCGGGCAG GGCTTTGATT CTGCAGATCC GTCTCGTATG
    AAGCTTAGGA CCCAAGGGAA TGGCAAAACA GTTCTTCCAT CCTCTGTTTA TAGAAATGGA
    ACCGGCATCA TCTATCATTA TCATGGAATG GTTTCCCAGA AGCTGGAGGG AGACAGTTCT
    CCATCTCGCA AAAGGAAGGC CAAAGTAGCC ATCACCAGTC TGCCACAAAA GACAAGCCAA
    GTGGCAGCGC TGCCTCAAGC AGATAAGATG GAATCCACAA GTGACTCACA CTTCTCCAGA
    CAGGACCGAT TGCCCTCAAT TCCAAAGGAC TGCACACTGG AATTAAAGGG ACTTCTCCAC
    TTCCACGAAA GCCTCCGGAA GCTGTACAAG TGTGATGGAA TTACCTGGAA AGCCTGGAGC
    CCCCAAACCG AGGAGGTGGA AGGTGAAGCC TGCCCAGCTG GGTGGCACCA TCATTCAGGC
    TACTGCCACT CTTTGGTCAC AGAGCAGAAG GGCACCTGGA GCACTGCTGC CCGAGCTTGC
    AAGGAACATC ACCGAGGCAG CCTTGTAACT GTTCTCTCCA AGCAGCACAT GCGATGGCTT
    TGGGACATCA GTGGAAGAAG GCCCTTCTGG ATAGGTTTGA ATGACCAAGT GCATGCTGGC
    CACTGGGAGT GGATCGGCGG GGAACCTGTC ACCTTCACCA ACTGGAGGAA AGGGCCCCCT
    CAACGTTTAA AGCATGGCAA GGACTGTGTT TTGGCTCAAA GACAAGGGAA ATGGCAAGTG
    CAGGACTGTA GGAAGGGCAA ACCTCACAAT TACGTGTGCT CCAGGAAACT CCAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281143.1
    CDS861..7280
    Translation

    Target ORF information:

    RefSeq Version XM_022425435.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GCGGCAAAGG ATTTCTCTTC ACTGTGGCTG ACCTTCAGGC TGGAGTTGTT
    CGCTATCATC ATGATGATAG TGACTCCACC AAAGATTTTG TGATCTTCAG GATATTCGAT
    GGCCATCACA GCATCCGCCA TAAGTTTCCC ATCAACATTT TGCCCAAAGA TGACAGTCCC
    CCATTTCTCA TAAGCAATGT TGTGATTGAA CTGGAGGAAG GGCAGACCAT ACTGATCCAG
    AGTTCTATGC TGAGAGCATC AGACATGGAC TCCAGTGATG ACTACATCTA TTTTAATATC
    ACGAAACCTC CTCAGGCTGG AGAGATCATG AAGAAGCCAG GGCCAGGACT GATAGGCTAT
    CCTGTCCCTG GCTTCCTTCA GAGGGATCTA TTTAATGGCA TCATTTACTA TCGTCACTTT
    GGTGGAGAGG TCTTTGAAGA CTCTTTTGAA TTTGTCCTGT GGGACAGCCA TGAACCTCCA
    AATTTCTCAG TGCCACAGGT GGTGACCATC CATATCACTC CAGTGGATGA CCAACTTCCA
    AAGGAGGCTC CTGGAGTTTC TCGGCATTTG GTTGTCAAGG AAACTGAGGT GGCCTACATA
    ACTAAGAAAC ATCTACATTT TATAGATACA GAATATAACA GGGAGCTTCT CTACACGATA
    ACGACACCTC CGTTTTTCTC TTTCGGCCAC AGACATCTGG ATGCTGGGAA ATTAATTATG
    GTGGATAGCA TTTCAAAGCT AACTAAAAAT TCTGCAGCCC GAGGGCTGAG ATCATTCACT
    CAGCATGCTG TAAACCACAT GAAAGTGGCC TACAAGCCAC CCATGCAAGA TATGGGCCTC
    AATCCAGGGT ATGTTCAGTT TGCATTTTCC ATCAGTAACC AGCATGGAGG CACTTTGCAT
    GGGATCTGCT TTAACATCAC AATTCTTCCA GTGGACAATC AAGTTCCTAA GGTGTTTACC
    AACCCCCTAA GAGTGGCTGA GGGAGCTCAA TGTGTCATCA GCACAGAAAA TGTTCTGATT
    TCTGATGTGG ATACCAAACT GGACAGCATC TACCTCTCCC TACAAAGACT GCCTCAGTGT
    GGAAGAGTGG AGCTGGATGG ATTTCCTCTA AACATAGGAG ACATATTTTC TTGGGGAGAC
    CTCCATGCCT TAAAAGTTAG GTATCAGCAT GATGGATCTG AGATACTTCA GGATGACATA
    TTCTTGGAGG TCACAGATGG CACGAATTCA GCAGAATTTA TTCTACACAT TGAGGTATTC
    CCTGTAAATG ATGAGCTACC AATCCTAAAG ACTGACCTCA TTCCCATGAT GCATTGCTCA
    GAGGGCGAAG AGGTAGTCAT CACCTCTGAA TACATTTTTG CTACTGATGT GGACAGTGAT
    GACTTGAAAC TGATGTTTGT GATTGCTCAA AAGCCTCAAC ATGGGGTGGT GAGGAAGGCT
    GGAGTCACAG TGGATCAGTT CTCTCAGGGA GATGTCATCT CGGGGGCTGT GACGTACAAA
    CACACGGGTG GTGAGATTGG CCTGGTGCCT TGTTTTGATA CCATTACATT GGTGGTTTCG
    GATGAAGAAG CTGGCCCTTT TGTGAATGGC TGTTGCTACA ATGGGCCTCA TCCATCTATT
    CCTCTTCATG AGTCCTTTCC AGTGTATGCC CTCAACATCA CAGTACATCC AGTGGACAAC
    CAGTCACCTT CAATCGCAAT AGGTACCATG TTTGTGGTAG ATGAGGGTTT CTCCGCAGCC
    GTTACCATTA ACCATTTGTC CGCCACTGAC CCTGACACTG CAACAGATGA CTTGGAATTT
    GTTTTGGTTT CTCCTCCCCA GTTTGGCTAC CTTGAAAACA CACTTCCTTC TGCAGGTTTT
    GAAAAAAGCA ATATGGGCAT AAGCATAGCT TCGTTCCCGT GGAGAGACAT GAAGGCTTTG
    CACATTAACT ATGTGCAGGC TAGGCATCTA AGGATGGAAC CGACGGCTGA CCAGTTCATG
    GTGTATGTCA CAGATGGGAA GCAACGCTCC TTGGAGATAC CATTTTACAT TATAATCAAC
    CCCATGAATG ATGAAGCTCC TGACTTTGTA GTGCAGAATA TTACTGTGTG TGAGGGTCAG
    ATGAAAGAAC TGGAATCCTC CACCATCAAT GCAGCTGACC TGGATGTTCC CAAAGACCCC
    TTGCTGTTCA GCATCACTCA TAAACCACGC CATGGCCTCC TCATCAACAG GGTGCTTAGC
    AAAGACTTTC CTCAAAATAA GCAGTCTGTC AACCCTGGCC AGAAGCACGA ACTTGTTCAG
    AACTTTTCCT TGGAACTTCT CAAGAAAGGA ATGAAGTTGA TGTATGTGCA TGATGACTCA
    GAGACTCTTG CTGATGACTT TACAATCCAG TTGTCAGATG GGAAACATAA GATATTAAAA
    ACCATTTCAG TAGAGATCAC CCCAGTTAAT GATGAGAAAC CAATGCTGAG CAAGAAGGCT
    GAAATTACAA TGAAAATGGG TGAAACTCGA ATTATTTCTA GTGCTGTTCT TTCAGCCATA
    GATAAAGACT CACCCAGGGA GAAGATTTAC TATTTATTTG AAAGGCTTCC ACAAAATGGG
    CAACTTCAAC TTAAGACAGG GAGGGACTGG GTCCCTCTCT ACCCTGGCAT GAAGTGCACT
    CAGGAAGAAG TGGATTTGAA CTTGCTGAGA TACACACACA CCGGGGCAAT GGATTCCCAG
    GACCAAGACA GCTTTACCTT CTACCTCTGG GATGGTGACA ACAGATCACC GGCATTTGAC
    TGTCACATCA CCATCAAGGA TATGGGAAAA GGTGATATTG TCATCCTTGT GAAACCGCTG
    GTGGTGTCTA AAGGTGATCG AGGTTTCTTG ACGACCACCA CTCTCCTTGC TGTGGATGGA
    ACAGACAAAC CTGAGGAACT ACTCTATGTC ATCACCTCCC CTCCGCGGTA TGGCCAGGTC
    CAGTATGTCC GCTATCCTGG AGTTCCCATT ACAAGCTTCA GCCAAATGGA CATAGCAGGA
    CAGACAGTCT GCTATGTACA TAAGAGTAAG GCAGCTGTCT CCCGTGACAC CTTCAGATTC
    ATCGTCAGCA ATGGCCTGCA GACCAAACGT GGGGTGTTTG AGATCATACT GGAGACCATG
    GACAGAGCCT TACCCGTGGT GACCAGGAAC AGGGGGCTGA GACTGGACGA AGGGACCACG
    GGCCTGCTCT CCCCTGACAT CCTCCAGCTG ACCGACCCTG ACACCCCAGC GGAGAACCTC
    ACCTTCATGT TGGCCCAGCT CCCACAACAC GGCCAGCTTT ACCTGCGGGG GACAGTGCTG
    CTTCAACACA ACTTCACCCA GCAGGACGTG GACAGCAGGA ATGTGGCCTA TAGGCACTTG
    GGAGGAGACT CCCAGGTGGA CTGCTTTACG TTTGTGGCCA CCGATAGTAC GAACCAAGGC
    TTCGTTGTGG ATGGGAGAGT GGGACAAGAA CCTGTATCAT TCACCATCCA GGTTGACCAG
    TTGGACAAAA CAGCCCCCCA CATCACACAT TTGCATTCCC CTTCTCAAGT GGTGCTCCTG
    AAAAATGGCT GTTACGGGAT TTACCTCACT TCCCATGTGC TGAAGGCATC GGACCCTGAC
    ACCAATGACG ATCTGATCAT CTTTAAGATT TTACGAGGCC CCCAACATGG ACATCTGGAG
    AACACAACCA CAGGTGAATT TATCCATGAG AAATTTGTCC AAAAGGACTT AAACAGTAAG
    ACCATTCTTT ACGTCATAAA CCCATCTTTG GATGTGAATT CAGATATCAT GGAATTCCAA
    ATCATGGACC CCACAGGGAA CTCTGCTACT CCTCAAATTT TGGAACTGAA ATGGTCTCAT
    ATTGAGTGGT CACAGACGGA ATTTGAGGTC TGCGAGAGTA TGGGCATGTT GCCCTTGGAA
    ATTACCAGAA GGGGATATTC CATGGACTCA GCCTTCGTGA GTGTAAAGGT CAACCAGGTG
    TCAGCTACAG TTGGAAAAGA CTTCACTCTG ACTCCATCTA AACTGATTCA GTTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGACGAT
    GAGGCCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCAG TTCTTGGCAC CAAGACAAAA
    GCTGCAGTGA AAATTTTGGA CTCAAAAGGA GGACAGTGTC ATCCTTCATA TTCCTTCAAC
    CCAAACAAGC ACAATGCAAG GGAGAAGGGC ATTTGGCACC CGCTGTCCCC AGGGTCTTCC
    TCATCCACCA CTTCTGGTTC CTTTCATCTG GAAAGAAGAC CCCTTCCATC TTCCAAGCGG
    CCAGCAGTCA CTAGGGGAGA CACCGGGCAG GGCTTTGATT CTGCAGATCC GTCTCGTATG
    AAGCTTAGGA CCCAAGGGAA TGGCAAAACA GTTCTTCCAT CCTCTGTTTA TAGAAATGGA
    ACCGGCATCA TCTATCATTA TCATGGAATG GTTTCCCAGA AGCTGGAGGG AGACAGTTCT
    CCATCTCGCA AAAGGAAGGC CAAAGTAGCC ATCACCAGTC TGCCACAAAA GACAAGCCAA
    GTGGCAGCGC TGCCTCAAGC AGATAAGATG GAATCCACAA GTGACTCACA CTTCTCCAGA
    CAGGACCGAT TGCCCTCAAT TCCAAAGGAC TGCACACTGG AATTAAAGGG ACTTCTCCAC
    TTCCACGAAA GCCTCCGGAA GCTGTACAAG TGTGATGGAA TTACCTGGAA AGCCTGGAGC
    CCCCAAACCG AGGAGGTGGA AGGTGAAGCC TGCCCAGCTG GGTGGCACCA TCATTCAGGC
    TACTGCCACT CTTTGGTCAC AGAGCAGAAG GGCACCTGGA GCACTGCTGC CCGAGCTTGC
    AAGGAACATC ACCGAGGCAG CCTTGTAACT GTTCTCTCCA AGCAGCACAT GCGATGGCTT
    TGGGACATCA GTGGAAGAAG GCCCTTCTGG ATAGGTTTGA ATGACCAAGT GCATGCTGGC
    CACTGGGAGT GGATCGGCGG GGAACCTGTC ACCTTCACCA ACTGGAGGAA AGGGCCCCCT
    CAACGTTTAA AGCATGGCAA GGACTGTGTT TTGGCTCAAA GACAAGGGAA ATGGCAAGTG
    CAGGACTGTA GGAAGGGCAA ACCTCACAAT TACGTGTGCT CCAGGAAACT CCAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe39154
    Clone ID Related Accession (Same CDS sequence) XM_022425435.1 , XM_014117771.1
    Accession Version XM_014117771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6420bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GCGGCAAAGG ATTTCTCTTC ACTGTGGCTG ACCTTCAGGC TGGAGTTGTT
    CGCTATCATC ATGATGATAG TGACTCCACC AAAGATTTTG TGATCTTCAG GATATTCGAT
    GGCCATCACA GCATCCGCCA TAAGTTTCCC ATCAACATTT TGCCCAAAGA TGACAGTCCC
    CCATTTCTCA TAAGCAATGT TGTGATTGAA CTGGAGGAAG GGCAGACCAT ACTGATCCAG
    AGTTCTATGC TGAGAGCATC AGACATGGAC TCCAGTGATG ACTACATCTA TTTTAATATC
    ACGAAACCTC CTCAGGCTGG AGAGATCATG AAGAAGCCAG GGCCAGGACT GATAGGCTAT
    CCTGTCCCTG GCTTCCTTCA GAGGGATCTA TTTAATGGCA TCATTTACTA TCGTCACTTT
    GGTGGAGAGG TCTTTGAAGA CTCTTTTGAA TTTGTCCTGT GGGACAGCCA TGAACCTCCA
    AATTTCTCAG TGCCACAGGT GGTGACCATC CATATCACTC CAGTGGATGA CCAACTTCCA
    AAGGAGGCTC CTGGAGTTTC TCGGCATTTG GTTGTCAAGG AAACTGAGGT GGCCTACATA
    ACTAAGAAAC ATCTACATTT TATAGATACA GAATATAACA GGGAGCTTCT CTACACGATA
    ACGACACCTC CGTTTTTCTC TTTCGGCCAC AGACATCTGG ATGCTGGGAA ATTAATTATG
    GTGGATAGCA TTTCAAAGCT AACTAAAAAT TCTGCAGCCC GAGGGCTGAG ATCATTCACT
    CAGCATGCTG TAAACCACAT GAAAGTGGCC TACAAGCCAC CCATGCAAGA TATGGGCCTC
    AATCCAGGGT ATGTTCAGTT TGCATTTTCC ATCAGTAACC AGCATGGAGG CACTTTGCAT
    GGGATCTGCT TTAACATCAC AATTCTTCCA GTGGACAATC AAGTTCCTAA GGTGTTTACC
    AACCCCCTAA GAGTGGCTGA GGGAGCTCAA TGTGTCATCA GCACAGAAAA TGTTCTGATT
    TCTGATGTGG ATACCAAACT GGACAGCATC TACCTCTCCC TACAAAGACT GCCTCAGTGT
    GGAAGAGTGG AGCTGGATGG ATTTCCTCTA AACATAGGAG ACATATTTTC TTGGGGAGAC
    CTCCATGCCT TAAAAGTTAG GTATCAGCAT GATGGATCTG AGATACTTCA GGATGACATA
    TTCTTGGAGG TCACAGATGG CACGAATTCA GCAGAATTTA TTCTACACAT TGAGGTATTC
    CCTGTAAATG ATGAGCTACC AATCCTAAAG ACTGACCTCA TTCCCATGAT GCATTGCTCA
    GAGGGCGAAG AGGTAGTCAT CACCTCTGAA TACATTTTTG CTACTGATGT GGACAGTGAT
    GACTTGAAAC TGATGTTTGT GATTGCTCAA AAGCCTCAAC ATGGGGTGGT GAGGAAGGCT
    GGAGTCACAG TGGATCAGTT CTCTCAGGGA GATGTCATCT CGGGGGCTGT GACGTACAAA
    CACACGGGTG GTGAGATTGG CCTGGTGCCT TGTTTTGATA CCATTACATT GGTGGTTTCG
    GATGAAGAAG CTGGCCCTTT TGTGAATGGC TGTTGCTACA ATGGGCCTCA TCCATCTATT
    CCTCTTCATG AGTCCTTTCC AGTGTATGCC CTCAACATCA CAGTACATCC AGTGGACAAC
    CAGTCACCTT CAATCGCAAT AGGTACCATG TTTGTGGTAG ATGAGGGTTT CTCCGCAGCC
    GTTACCATTA ACCATTTGTC CGCCACTGAC CCTGACACTG CAACAGATGA CTTGGAATTT
    GTTTTGGTTT CTCCTCCCCA GTTTGGCTAC CTTGAAAACA CACTTCCTTC TGCAGGTTTT
    GAAAAAAGCA ATATGGGCAT AAGCATAGCT TCGTTCCCGT GGAGAGACAT GAAGGCTTTG
    CACATTAACT ATGTGCAGGC TAGGCATCTA AGGATGGAAC CGACGGCTGA CCAGTTCATG
    GTGTATGTCA CAGATGGGAA GCAACGCTCC TTGGAGATAC CATTTTACAT TATAATCAAC
    CCCATGAATG ATGAAGCTCC TGACTTTGTA GTGCAGAATA TTACTGTGTG TGAGGGTCAG
    ATGAAAGAAC TGGAATCCTC CACCATCAAT GCAGCTGACC TGGATGTTCC CAAAGACCCC
    TTGCTGTTCA GCATCACTCA TAAACCACGC CATGGCCTCC TCATCAACAG GGTGCTTAGC
    AAAGACTTTC CTCAAAATAA GCAGTCTGTC AACCCTGGCC AGAAGCACGA ACTTGTTCAG
    AACTTTTCCT TGGAACTTCT CAAGAAAGGA ATGAAGTTGA TGTATGTGCA TGATGACTCA
    GAGACTCTTG CTGATGACTT TACAATCCAG TTGTCAGATG GGAAACATAA GATATTAAAA
    ACCATTTCAG TAGAGATCAC CCCAGTTAAT GATGAGAAAC CAATGCTGAG CAAGAAGGCT
    GAAATTACAA TGAAAATGGG TGAAACTCGA ATTATTTCTA GTGCTGTTCT TTCAGCCATA
    GATAAAGACT CACCCAGGGA GAAGATTTAC TATTTATTTG AAAGGCTTCC ACAAAATGGG
    CAACTTCAAC TTAAGACAGG GAGGGACTGG GTCCCTCTCT ACCCTGGCAT GAAGTGCACT
    CAGGAAGAAG TGGATTTGAA CTTGCTGAGA TACACACACA CCGGGGCAAT GGATTCCCAG
    GACCAAGACA GCTTTACCTT CTACCTCTGG GATGGTGACA ACAGATCACC GGCATTTGAC
    TGTCACATCA CCATCAAGGA TATGGGAAAA GGTGATATTG TCATCCTTGT GAAACCGCTG
    GTGGTGTCTA AAGGTGATCG AGGTTTCTTG ACGACCACCA CTCTCCTTGC TGTGGATGGA
    ACAGACAAAC CTGAGGAACT ACTCTATGTC ATCACCTCCC CTCCGCGGTA TGGCCAGGTC
    CAGTATGTCC GCTATCCTGG AGTTCCCATT ACAAGCTTCA GCCAAATGGA CATAGCAGGA
    CAGACAGTCT GCTATGTACA TAAGAGTAAG GCAGCTGTCT CCCGTGACAC CTTCAGATTC
    ATCGTCAGCA ATGGCCTGCA GACCAAACGT GGGGTGTTTG AGATCATACT GGAGACCATG
    GACAGAGCCT TACCCGTGGT GACCAGGAAC AGGGGGCTGA GACTGGACGA AGGGACCACG
    GGCCTGCTCT CCCCTGACAT CCTCCAGCTG ACCGACCCTG ACACCCCAGC GGAGAACCTC
    ACCTTCATGT TGGCCCAGCT CCCACAACAC GGCCAGCTTT ACCTGCGGGG GACAGTGCTG
    CTTCAACACA ACTTCACCCA GCAGGACGTG GACAGCAGGA ATGTGGCCTA TAGGCACTTG
    GGAGGAGACT CCCAGGTGGA CTGCTTTACG TTTGTGGCCA CCGATAGTAC GAACCAAGGC
    TTCGTTGTGG ATGGGAGAGT GGGACAAGAA CCTGTATCAT TCACCATCCA GGTTGACCAG
    TTGGACAAAA CAGCCCCCCA CATCACACAT TTGCATTCCC CTTCTCAAGT GGTGCTCCTG
    AAAAATGGCT GTTACGGGAT TTACCTCACT TCCCATGTGC TGAAGGCATC GGACCCTGAC
    ACCAATGACG ATCTGATCAT CTTTAAGATT TTACGAGGCC CCCAACATGG ACATCTGGAG
    AACACAACCA CAGGTGAATT TATCCATGAG AAATTTGTCC AAAAGGACTT AAACAGTAAG
    ACCATTCTTT ACGTCATAAA CCCATCTTTG GATGTGAATT CAGATATCAT GGAATTCCAA
    ATCATGGACC CCACAGGGAA CTCTGCTACT CCTCAAATTT TGGAACTGAA ATGGTCTCAT
    ATTGAGTGGT CACAGACGGA ATTTGAGGTC TGCGAGAGTA TGGGCATGTT GCCCTTGGAA
    ATTACCAGAA GGGGATATTC CATGGACTCA GCCTTCGTGA GTGTAAAGGT CAACCAGGTG
    TCAGCTACAG TTGGAAAAGA CTTCACTCTG ACTCCATCTA AACTGATTCA GTTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGACGAT
    GAGGCCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCAG TTCTTGGCAC CAAGACAAAA
    GCTGCAGTGA AAATTTTGGA CTCAAAAGGA GGACAGTGTC ATCCTTCATA TTCCTTCAAC
    CCAAACAAGC ACAATGCAAG GGAGAAGGGC ATTTGGCACC CGCTGTCCCC AGGGTCTTCC
    TCATCCACCA CTTCTGGTTC CTTTCATCTG GAAAGAAGAC CCCTTCCATC TTCCAAGCGG
    CCAGCAGTCA CTAGGGGAGA CACCGGGCAG GGCTTTGATT CTGCAGATCC GTCTCGTATG
    AAGCTTAGGA CCCAAGGGAA TGGCAAAACA GTTCTTCCAT CCTCTGTTTA TAGAAATGGA
    ACCGGCATCA TCTATCATTA TCATGGAATG GTTTCCCAGA AGCTGGAGGG AGACAGTTCT
    CCATCTCGCA AAAGGAAGGC CAAAGTAGCC ATCACCAGTC TGCCACAAAA GACAAGCCAA
    GTGGCAGCGC TGCCTCAAGC AGATAAGATG GAATCCACAA GTGACTCACA CTTCTCCAGA
    CAGGACCGAT TGCCCTCAAT TCCAAAGGAC TGCACACTGG AATTAAAGGG ACTTCTCCAC
    TTCCACGAAA GCCTCCGGAA GCTGTACAAG TGTGATGGAA TTACCTGGAA AGCCTGGAGC
    CCCCAAACCG AGGAGGTGGA AGGTGAAGCC TGCCCAGCTG GGTGGCACCA TCATTCAGGC
    TACTGCCACT CTTTGGTCAC AGAGCAGAAG GGCACCTGGA GCACTGCTGC CCGAGCTTGC
    AAGGAACATC ACCGAGGCAG CCTTGTAACT GTTCTCTCCA AGCAGCACAT GCGATGGCTT
    TGGGACATCA GTGGAAGAAG GCCCTTCTGG ATAGGTTTGA ATGACCAAGT GCATGCTGGC
    CACTGGGAGT GGATCGGCGG GGAACCTGTC ACCTTCACCA ACTGGAGGAA AGGGCCCCCT
    CAACGTTTAA AGCATGGCAA GGACTGTGTT TTGGCTCAAA GACAAGGGAA ATGGCAAGTG
    CAGGACTGTA GGAAGGGCAA ACCTCACAAT TACGTGTGCT CCAGGAAACT CCAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973246.1
    CDS1042..7461
    Translation

    Target ORF information:

    RefSeq Version XM_014117771.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GCGGCAAAGG ATTTCTCTTC ACTGTGGCTG ACCTTCAGGC TGGAGTTGTT
    CGCTATCATC ATGATGATAG TGACTCCACC AAAGATTTTG TGATCTTCAG GATATTCGAT
    GGCCATCACA GCATCCGCCA TAAGTTTCCC ATCAACATTT TGCCCAAAGA TGACAGTCCC
    CCATTTCTCA TAAGCAATGT TGTGATTGAA CTGGAGGAAG GGCAGACCAT ACTGATCCAG
    AGTTCTATGC TGAGAGCATC AGACATGGAC TCCAGTGATG ACTACATCTA TTTTAATATC
    ACGAAACCTC CTCAGGCTGG AGAGATCATG AAGAAGCCAG GGCCAGGACT GATAGGCTAT
    CCTGTCCCTG GCTTCCTTCA GAGGGATCTA TTTAATGGCA TCATTTACTA TCGTCACTTT
    GGTGGAGAGG TCTTTGAAGA CTCTTTTGAA TTTGTCCTGT GGGACAGCCA TGAACCTCCA
    AATTTCTCAG TGCCACAGGT GGTGACCATC CATATCACTC CAGTGGATGA CCAACTTCCA
    AAGGAGGCTC CTGGAGTTTC TCGGCATTTG GTTGTCAAGG AAACTGAGGT GGCCTACATA
    ACTAAGAAAC ATCTACATTT TATAGATACA GAATATAACA GGGAGCTTCT CTACACGATA
    ACGACACCTC CGTTTTTCTC TTTCGGCCAC AGACATCTGG ATGCTGGGAA ATTAATTATG
    GTGGATAGCA TTTCAAAGCT AACTAAAAAT TCTGCAGCCC GAGGGCTGAG ATCATTCACT
    CAGCATGCTG TAAACCACAT GAAAGTGGCC TACAAGCCAC CCATGCAAGA TATGGGCCTC
    AATCCAGGGT ATGTTCAGTT TGCATTTTCC ATCAGTAACC AGCATGGAGG CACTTTGCAT
    GGGATCTGCT TTAACATCAC AATTCTTCCA GTGGACAATC AAGTTCCTAA GGTGTTTACC
    AACCCCCTAA GAGTGGCTGA GGGAGCTCAA TGTGTCATCA GCACAGAAAA TGTTCTGATT
    TCTGATGTGG ATACCAAACT GGACAGCATC TACCTCTCCC TACAAAGACT GCCTCAGTGT
    GGAAGAGTGG AGCTGGATGG ATTTCCTCTA AACATAGGAG ACATATTTTC TTGGGGAGAC
    CTCCATGCCT TAAAAGTTAG GTATCAGCAT GATGGATCTG AGATACTTCA GGATGACATA
    TTCTTGGAGG TCACAGATGG CACGAATTCA GCAGAATTTA TTCTACACAT TGAGGTATTC
    CCTGTAAATG ATGAGCTACC AATCCTAAAG ACTGACCTCA TTCCCATGAT GCATTGCTCA
    GAGGGCGAAG AGGTAGTCAT CACCTCTGAA TACATTTTTG CTACTGATGT GGACAGTGAT
    GACTTGAAAC TGATGTTTGT GATTGCTCAA AAGCCTCAAC ATGGGGTGGT GAGGAAGGCT
    GGAGTCACAG TGGATCAGTT CTCTCAGGGA GATGTCATCT CGGGGGCTGT GACGTACAAA
    CACACGGGTG GTGAGATTGG CCTGGTGCCT TGTTTTGATA CCATTACATT GGTGGTTTCG
    GATGAAGAAG CTGGCCCTTT TGTGAATGGC TGTTGCTACA ATGGGCCTCA TCCATCTATT
    CCTCTTCATG AGTCCTTTCC AGTGTATGCC CTCAACATCA CAGTACATCC AGTGGACAAC
    CAGTCACCTT CAATCGCAAT AGGTACCATG TTTGTGGTAG ATGAGGGTTT CTCCGCAGCC
    GTTACCATTA ACCATTTGTC CGCCACTGAC CCTGACACTG CAACAGATGA CTTGGAATTT
    GTTTTGGTTT CTCCTCCCCA GTTTGGCTAC CTTGAAAACA CACTTCCTTC TGCAGGTTTT
    GAAAAAAGCA ATATGGGCAT AAGCATAGCT TCGTTCCCGT GGAGAGACAT GAAGGCTTTG
    CACATTAACT ATGTGCAGGC TAGGCATCTA AGGATGGAAC CGACGGCTGA CCAGTTCATG
    GTGTATGTCA CAGATGGGAA GCAACGCTCC TTGGAGATAC CATTTTACAT TATAATCAAC
    CCCATGAATG ATGAAGCTCC TGACTTTGTA GTGCAGAATA TTACTGTGTG TGAGGGTCAG
    ATGAAAGAAC TGGAATCCTC CACCATCAAT GCAGCTGACC TGGATGTTCC CAAAGACCCC
    TTGCTGTTCA GCATCACTCA TAAACCACGC CATGGCCTCC TCATCAACAG GGTGCTTAGC
    AAAGACTTTC CTCAAAATAA GCAGTCTGTC AACCCTGGCC AGAAGCACGA ACTTGTTCAG
    AACTTTTCCT TGGAACTTCT CAAGAAAGGA ATGAAGTTGA TGTATGTGCA TGATGACTCA
    GAGACTCTTG CTGATGACTT TACAATCCAG TTGTCAGATG GGAAACATAA GATATTAAAA
    ACCATTTCAG TAGAGATCAC CCCAGTTAAT GATGAGAAAC CAATGCTGAG CAAGAAGGCT
    GAAATTACAA TGAAAATGGG TGAAACTCGA ATTATTTCTA GTGCTGTTCT TTCAGCCATA
    GATAAAGACT CACCCAGGGA GAAGATTTAC TATTTATTTG AAAGGCTTCC ACAAAATGGG
    CAACTTCAAC TTAAGACAGG GAGGGACTGG GTCCCTCTCT ACCCTGGCAT GAAGTGCACT
    CAGGAAGAAG TGGATTTGAA CTTGCTGAGA TACACACACA CCGGGGCAAT GGATTCCCAG
    GACCAAGACA GCTTTACCTT CTACCTCTGG GATGGTGACA ACAGATCACC GGCATTTGAC
    TGTCACATCA CCATCAAGGA TATGGGAAAA GGTGATATTG TCATCCTTGT GAAACCGCTG
    GTGGTGTCTA AAGGTGATCG AGGTTTCTTG ACGACCACCA CTCTCCTTGC TGTGGATGGA
    ACAGACAAAC CTGAGGAACT ACTCTATGTC ATCACCTCCC CTCCGCGGTA TGGCCAGGTC
    CAGTATGTCC GCTATCCTGG AGTTCCCATT ACAAGCTTCA GCCAAATGGA CATAGCAGGA
    CAGACAGTCT GCTATGTACA TAAGAGTAAG GCAGCTGTCT CCCGTGACAC CTTCAGATTC
    ATCGTCAGCA ATGGCCTGCA GACCAAACGT GGGGTGTTTG AGATCATACT GGAGACCATG
    GACAGAGCCT TACCCGTGGT GACCAGGAAC AGGGGGCTGA GACTGGACGA AGGGACCACG
    GGCCTGCTCT CCCCTGACAT CCTCCAGCTG ACCGACCCTG ACACCCCAGC GGAGAACCTC
    ACCTTCATGT TGGCCCAGCT CCCACAACAC GGCCAGCTTT ACCTGCGGGG GACAGTGCTG
    CTTCAACACA ACTTCACCCA GCAGGACGTG GACAGCAGGA ATGTGGCCTA TAGGCACTTG
    GGAGGAGACT CCCAGGTGGA CTGCTTTACG TTTGTGGCCA CCGATAGTAC GAACCAAGGC
    TTCGTTGTGG ATGGGAGAGT GGGACAAGAA CCTGTATCAT TCACCATCCA GGTTGACCAG
    TTGGACAAAA CAGCCCCCCA CATCACACAT TTGCATTCCC CTTCTCAAGT GGTGCTCCTG
    AAAAATGGCT GTTACGGGAT TTACCTCACT TCCCATGTGC TGAAGGCATC GGACCCTGAC
    ACCAATGACG ATCTGATCAT CTTTAAGATT TTACGAGGCC CCCAACATGG ACATCTGGAG
    AACACAACCA CAGGTGAATT TATCCATGAG AAATTTGTCC AAAAGGACTT AAACAGTAAG
    ACCATTCTTT ACGTCATAAA CCCATCTTTG GATGTGAATT CAGATATCAT GGAATTCCAA
    ATCATGGACC CCACAGGGAA CTCTGCTACT CCTCAAATTT TGGAACTGAA ATGGTCTCAT
    ATTGAGTGGT CACAGACGGA ATTTGAGGTC TGCGAGAGTA TGGGCATGTT GCCCTTGGAA
    ATTACCAGAA GGGGATATTC CATGGACTCA GCCTTCGTGA GTGTAAAGGT CAACCAGGTG
    TCAGCTACAG TTGGAAAAGA CTTCACTCTG ACTCCATCTA AACTGATTCA GTTTGACCCA
    GGAATGTCAA CTAAGATGTG GAATATAGCA ATTACCTATG ACGGATTAGA GGAAGACGAT
    GAGGCCTTTG AAGTAATTCT GAACTCCCCT GTGAATGCAG TTCTTGGCAC CAAGACAAAA
    GCTGCAGTGA AAATTTTGGA CTCAAAAGGA GGACAGTGTC ATCCTTCATA TTCCTTCAAC
    CCAAACAAGC ACAATGCAAG GGAGAAGGGC ATTTGGCACC CGCTGTCCCC AGGGTCTTCC
    TCATCCACCA CTTCTGGTTC CTTTCATCTG GAAAGAAGAC CCCTTCCATC TTCCAAGCGG
    CCAGCAGTCA CTAGGGGAGA CACCGGGCAG GGCTTTGATT CTGCAGATCC GTCTCGTATG
    AAGCTTAGGA CCCAAGGGAA TGGCAAAACA GTTCTTCCAT CCTCTGTTTA TAGAAATGGA
    ACCGGCATCA TCTATCATTA TCATGGAATG GTTTCCCAGA AGCTGGAGGG AGACAGTTCT
    CCATCTCGCA AAAGGAAGGC CAAAGTAGCC ATCACCAGTC TGCCACAAAA GACAAGCCAA
    GTGGCAGCGC TGCCTCAAGC AGATAAGATG GAATCCACAA GTGACTCACA CTTCTCCAGA
    CAGGACCGAT TGCCCTCAAT TCCAAAGGAC TGCACACTGG AATTAAAGGG ACTTCTCCAC
    TTCCACGAAA GCCTCCGGAA GCTGTACAAG TGTGATGGAA TTACCTGGAA AGCCTGGAGC
    CCCCAAACCG AGGAGGTGGA AGGTGAAGCC TGCCCAGCTG GGTGGCACCA TCATTCAGGC
    TACTGCCACT CTTTGGTCAC AGAGCAGAAG GGCACCTGGA GCACTGCTGC CCGAGCTTGC
    AAGGAACATC ACCGAGGCAG CCTTGTAACT GTTCTCTCCA AGCAGCACAT GCGATGGCTT
    TGGGACATCA GTGGAAGAAG GCCCTTCTGG ATAGGTTTGA ATGACCAAGT GCATGCTGGC
    CACTGGGAGT GGATCGGCGG GGAACCTGTC ACCTTCACCA ACTGGAGGAA AGGGCCCCCT
    CAACGTTTAA AGCATGGCAA GGACTGTGTT TTGGCTCAAA GACAAGGGAA ATGGCAAGTG
    CAGGACTGTA GGAAGGGCAA ACCTCACAAT TACGTGTGCT CCAGGAAACT CCAGATGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_005626636.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626693.1
    CDS1042..7593
    Translation

    Target ORF information:

    RefSeq Version XM_005626636.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626636.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_022425434.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281142.1
    CDS1064..7615
    Translation

    Target ORF information:

    RefSeq Version XM_022425434.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425434.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_014117770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973245.1
    CDS574..7125
    Translation

    Target ORF information:

    RefSeq Version XM_014117770.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_005626638.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626695.1
    CDS480..7031
    Translation

    Target ORF information:

    RefSeq Version XM_005626638.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626638.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_005626639.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626696.1
    CDS367..6918
    Translation

    Target ORF information:

    RefSeq Version XM_005626639.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626639.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_014117769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013973244.1
    CDS1247..7798
    Translation

    Target ORF information:

    RefSeq Version XM_014117769.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014117769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_538668.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538668.2
    CDS286..6837
    Translation

    Target ORF information:

    RefSeq Version XM_538668.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_538668.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14408
    Clone ID Related Accession (Same CDS sequence) XM_005626636.2 , XM_022425434.1 , XM_014117770.1 , XM_005626638.2 , XM_005626639.2 , XM_014117769.1 , XM_538668.4 , XM_022425433.1
    Accession Version XM_022425433.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281141.1
    CDS857..7408
    Translation

    Target ORF information:

    RefSeq Version XM_022425433.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425433.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA TCACCGAGGC AGCCTTGTAA CTGTTCTCTC CAAGCAGCAC
    ATGCGATGGC TTTGGGACAT CAGTGGAAGA AGGCCCTTCT GGATAGGTTT GAATGACCAA
    GTGCATGCTG GCCACTGGGA GTGGATCGGC GGGGAACCTG TCACCTTCAC CAACTGGAGG
    AAAGGGCCCC CTCAACGTTT AAAGCATGGC AAGGACTGTG TTTTGGCTCA AAGACAAGGG
    AAATGGCAAG TGCAGGACTG TAGGAAGGGC AAACCTCACA ATTACGTGTG CTCCAGGAAA
    CTCCAGATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14409
    Clone ID Related Accession (Same CDS sequence) XM_022425436.1 , XM_005626640.2
    Accession Version XM_022425436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006593.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA AATAAAACTG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022281144.1
    CDS866..7141
    Translation

    Target ORF information:

    RefSeq Version XM_022425436.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022425436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA AATAAAACTG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe14409
    Clone ID Related Accession (Same CDS sequence) XM_022425436.1 , XM_005626640.2
    Accession Version XM_005626640.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product FRAS1-related extracellular matrix protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005626640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA AATAAAACTG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005626697.1
    CDS1043..7318
    Translation

    Target ORF information:

    RefSeq Version XM_005626640.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris FRAS1 related extracellular matrix 1 (FREM1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005626640.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGAGCTCTC CGAATTGGAG TGGGCACAGT GCCTTGCTGT TGTTGCTGCT GCTGCTTCTC 
    CTAAACTGGG CCAGTCCCTC CTTCATCAGC CTTAACCGTG GGGTCAGGGT GATGAAGGGC
    AGCTCTGCCT TCCTGTTGGG AGATGACCTG AAGTTTGCCA TCCCCAAAGA AAAAGATGCT
    TGCAAAGTGG AAGTTGTGAT GAACGAGCCA ATGACCCAAA GGGTCGGGAA ACTCACTCCA
    CAGGTCTTTG ACTGCCATTT CCTTCCCAAT GAAGTAAAGT ATATTCACAA TGGTTGCCCG
    ATTCTTGATG AAGATACAGT GAAGCTTAGA CTTTACAGAT TTACTGAGAC AGATACCTTC
    ACGGAAACCT TTATCCTGAG GGTCTATCTC CTGGAACCAG ACTGTAACAT CATCCGTAAG
    AGTAACAATG TACTGGAGGT GTCAGAATTC TACGGCCTGT CCGGAGCCAT TGATAAGAAC
    CTGCTCAGAT TTGATTACGA GAGGACGGCT GGCCTGGAGT GTACGGTCAG GCTGGATCCC
    GTGGGGAGCC GGCTGCCAGC CCACGGTCAG GTGGTCCTGC TGGAGCCTCG GCCAGAGAAA
    CCTCGGGGAG ATCAACCACA CAGTTTTTTC CCCAAGTCTC AACTGGGAAC AGAGCTGAGG
    TGTCCAGGTG GAAGCTGTGC CCTGGGACTG AAGAAAATGG GAAGTGTCAA TGTGAGCTGT
    GAGGAGTTCC TGTTGATGGG GCTTCATTAT CAACACACAG ATCCCCCTTC TCCCAACATC
    GACTACATCT CCATCCAGGT GGACCTCACA GATGGCAGGA GCAAAATCGT ATACAAGTCA
    GAGAGGGCGT GGTTGCCCGT CTATATCAGA CCTGGCATTC CTAATCAGAT TCCAAGGGCT
    GCGTTCATGG CCATGTTTAC TCTGGAAGTG GATCAGTTCA TCCTGACCTC TTTGACTACA
    TCCGTTCTGG ATTGTGAAGA GGACGAGACC CCGAAGTCCT TGCTGGTGTT TAACATTACT
    AAAGCGCCAC TCCAGGGCTA CGTGACTCAT CTCTGGGATC ACACCAGACC GGTCTCCTCA
    TTCACCTGGA AAGATCTCAG TGACCTGCAG ATAGCCTATC AACCACCGAA TAGCAGCCAT
    TCTGTGCGGA GACATTACGA GGTGGAGGTG GAGGTTTACG ACTTCTTCTT TGAAAGGAGT
    GCGCCTATTA CGGTCCACAT CTCCATCAGA ACAGCAGATA CAAATGCCCC GAGAGTGTCC
    TGGAACACAG GTCTGAGTCT TCTAGAGGGG CAATCTCGGG CCATCACTTG GGAACAGTTT
    CAGATTGTCG ACAATGATGA CATCCATGCT GTCCAGCTGG TCACCGTGGG CGGCCTGCAG
    CATGGACAAC TTACACTCAG AGGCGGCAAA GGATTTCTCT TCACTGTGGC TGACCTTCAG
    GCTGGAGTTG TTCGCTATCA TCATGATGAT AGTGACTCCA CCAAAGATTT TGTGATCTTC
    AGGATATTCG ATGGCCATCA CAGCATCCGC CATAAGTTTC CCATCAACAT TTTGCCCAAA
    GATGACAGTC CCCCATTTCT CATAAGCAAT GTTGTGATTG AACTGGAGGA AGGGCAGACC
    ATACTGATCC AGAGTTCTAT GCTGAGAGCA TCAGACATGG ACTCCAGTGA TGACTACATC
    TATTTTAATA TCACGAAACC TCCTCAGGCT GGAGAGATCA TGAAGAAGCC AGGGCCAGGA
    CTGATAGGCT ATCCTGTCCC TGGCTTCCTT CAGAGGGATC TATTTAATGG CATCATTTAC
    TATCGTCACT TTGGTGGAGA GGTCTTTGAA GACTCTTTTG AATTTGTCCT GTGGGACAGC
    CATGAACCTC CAAATTTCTC AGTGCCACAG GTGGTGACCA TCCATATCAC TCCAGTGGAT
    GACCAACTTC CAAAGGAGGC TCCTGGAGTT TCTCGGCATT TGGTTGTCAA GGAAACTGAG
    GTGGCCTACA TAACTAAGAA ACATCTACAT TTTATAGATA CAGAATATAA CAGGGAGCTT
    CTCTACACGA TAACGACACC TCCGTTTTTC TCTTTCGGCC ACAGACATCT GGATGCTGGG
    AAATTAATTA TGGTGGATAG CATTTCAAAG CTAACTAAAA ATTCTGCAGC CCGAGGGCTG
    AGATCATTCA CTCAGCATGC TGTAAACCAC ATGAAAGTGG CCTACAAGCC ACCCATGCAA
    GATATGGGCC TCAATCCAGG GTATGTTCAG TTTGCATTTT CCATCAGTAA CCAGCATGGA
    GGCACTTTGC ATGGGATCTG CTTTAACATC ACAATTCTTC CAGTGGACAA TCAAGTTCCT
    AAGGTGTTTA CCAACCCCCT AAGAGTGGCT GAGGGAGCTC AATGTGTCAT CAGCACAGAA
    AATGTTCTGA TTTCTGATGT GGATACCAAA CTGGACAGCA TCTACCTCTC CCTACAAAGA
    CTGCCTCAGT GTGGAAGAGT GGAGCTGGAT GGATTTCCTC TAAACATAGG AGACATATTT
    TCTTGGGGAG ACCTCCATGC CTTAAAAGTT AGGTATCAGC ATGATGGATC TGAGATACTT
    CAGGATGACA TATTCTTGGA GGTCACAGAT GGCACGAATT CAGCAGAATT TATTCTACAC
    ATTGAGGTAT TCCCTGTAAA TGATGAGCTA CCAATCCTAA AGACTGACCT CATTCCCATG
    ATGCATTGCT CAGAGGGCGA AGAGGTAGTC ATCACCTCTG AATACATTTT TGCTACTGAT
    GTGGACAGTG ATGACTTGAA ACTGATGTTT GTGATTGCTC AAAAGCCTCA ACATGGGGTG
    GTGAGGAAGG CTGGAGTCAC AGTGGATCAG TTCTCTCAGG GAGATGTCAT CTCGGGGGCT
    GTGACGTACA AACACACGGG TGGTGAGATT GGCCTGGTGC CTTGTTTTGA TACCATTACA
    TTGGTGGTTT CGGATGAAGA AGCTGGCCCT TTTGTGAATG GCTGTTGCTA CAATGGGCCT
    CATCCATCTA TTCCTCTTCA TGAGTCCTTT CCAGTGTATG CCCTCAACAT CACAGTACAT
    CCAGTGGACA ACCAGTCACC TTCAATCGCA ATAGGTACCA TGTTTGTGGT AGATGAGGGT
    TTCTCCGCAG CCGTTACCAT TAACCATTTG TCCGCCACTG ACCCTGACAC TGCAACAGAT
    GACTTGGAAT TTGTTTTGGT TTCTCCTCCC CAGTTTGGCT ACCTTGAAAA CACACTTCCT
    TCTGCAGGTT TTGAAAAAAG CAATATGGGC ATAAGCATAG CTTCGTTCCC GTGGAGAGAC
    ATGAAGGCTT TGCACATTAA CTATGTGCAG GCTAGGCATC TAAGGATGGA ACCGACGGCT
    GACCAGTTCA TGGTGTATGT CACAGATGGG AAGCAACGCT CCTTGGAGAT ACCATTTTAC
    ATTATAATCA ACCCCATGAA TGATGAAGCT CCTGACTTTG TAGTGCAGAA TATTACTGTG
    TGTGAGGGTC AGATGAAAGA ACTGGAATCC TCCACCATCA ATGCAGCTGA CCTGGATGTT
    CCCAAAGACC CCTTGCTGTT CAGCATCACT CATAAACCAC GCCATGGCCT CCTCATCAAC
    AGGGTGCTTA GCAAAGACTT TCCTCAAAAT AAGCAGTCTG TCAACCCTGG CCAGAAGCAC
    GAACTTGTTC AGAACTTTTC CTTGGAACTT CTCAAGAAAG GAATGAAGTT GATGTATGTG
    CATGATGACT CAGAGACTCT TGCTGATGAC TTTACAATCC AGTTGTCAGA TGGGAAACAT
    AAGATATTAA AAACCATTTC AGTAGAGATC ACCCCAGTTA ATGATGAGAA ACCAATGCTG
    AGCAAGAAGG CTGAAATTAC AATGAAAATG GGTGAAACTC GAATTATTTC TAGTGCTGTT
    CTTTCAGCCA TAGATAAAGA CTCACCCAGG GAGAAGATTT ACTATTTATT TGAAAGGCTT
    CCACAAAATG GGCAACTTCA ACTTAAGACA GGGAGGGACT GGGTCCCTCT CTACCCTGGC
    ATGAAGTGCA CTCAGGAAGA AGTGGATTTG AACTTGCTGA GATACACACA CACCGGGGCA
    ATGGATTCCC AGGACCAAGA CAGCTTTACC TTCTACCTCT GGGATGGTGA CAACAGATCA
    CCGGCATTTG ACTGTCACAT CACCATCAAG GATATGGGAA AAGGTGATAT TGTCATCCTT
    GTGAAACCGC TGGTGGTGTC TAAAGGTGAT CGAGGTTTCT TGACGACCAC CACTCTCCTT
    GCTGTGGATG GAACAGACAA ACCTGAGGAA CTACTCTATG TCATCACCTC CCCTCCGCGG
    TATGGCCAGG TCCAGTATGT CCGCTATCCT GGAGTTCCCA TTACAAGCTT CAGCCAAATG
    GACATAGCAG GACAGACAGT CTGCTATGTA CATAAGAGTA AGGCAGCTGT CTCCCGTGAC
    ACCTTCAGAT TCATCGTCAG CAATGGCCTG CAGACCAAAC GTGGGGTGTT TGAGATCATA
    CTGGAGACCA TGGACAGAGC CTTACCCGTG GTGACCAGGA ACAGGGGGCT GAGACTGGAC
    GAAGGGACCA CGGGCCTGCT CTCCCCTGAC ATCCTCCAGC TGACCGACCC TGACACCCCA
    GCGGAGAACC TCACCTTCAT GTTGGCCCAG CTCCCACAAC ACGGCCAGCT TTACCTGCGG
    GGGACAGTGC TGCTTCAACA CAACTTCACC CAGCAGGACG TGGACAGCAG GAATGTGGCC
    TATAGGCACT TGGGAGGAGA CTCCCAGGTG GACTGCTTTA CGTTTGTGGC CACCGATAGT
    ACGAACCAAG GCTTCGTTGT GGATGGGAGA GTGGGACAAG AACCTGTATC ATTCACCATC
    CAGGTTGACC AGTTGGACAA AACAGCCCCC CACATCACAC ATTTGCATTC CCCTTCTCAA
    GTGGTGCTCC TGAAAAATGG CTGTTACGGG ATTTACCTCA CTTCCCATGT GCTGAAGGCA
    TCGGACCCTG ACACCAATGA CGATCTGATC ATCTTTAAGA TTTTACGAGG CCCCCAACAT
    GGACATCTGG AGAACACAAC CACAGGTGAA TTTATCCATG AGAAATTTGT CCAAAAGGAC
    TTAAACAGTA AGACCATTCT TTACGTCATA AACCCATCTT TGGATGTGAA TTCAGATATC
    ATGGAATTCC AAATCATGGA CCCCACAGGG AACTCTGCTA CTCCTCAAAT TTTGGAACTG
    AAATGGTCTC ATATTGAGTG GTCACAGACG GAATTTGAGG TCTGCGAGAG TATGGGCATG
    TTGCCCTTGG AAATTACCAG AAGGGGATAT TCCATGGACT CAGCCTTCGT GAGTGTAAAG
    GTCAACCAGG TGTCAGCTAC AGTTGGAAAA GACTTCACTC TGACTCCATC TAAACTGATT
    CAGTTTGACC CAGGAATGTC AACTAAGATG TGGAATATAG CAATTACCTA TGACGGATTA
    GAGGAAGACG ATGAGGCCTT TGAAGTAATT CTGAACTCCC CTGTGAATGC AGTTCTTGGC
    ACCAAGACAA AAGCTGCAGT GAAAATTTTG GACTCAAAAG GAGGACAGTG TCATCCTTCA
    TATTCCTTCA ACCCAAACAA GCACAATGCA AGGGAGAAGG GCATTTGGCA CCCGCTGTCC
    CCAGGGTCTT CCTCATCCAC CACTTCTGGT TCCTTTCATC TGGAAAGAAG ACCCCTTCCA
    TCTTCCAAGC GGCCAGCAGT CACTAGGGGA GACACCGGGC AGGGCTTTGA TTCTGCAGAT
    CCGTCTCGTA TGAAGCTTAG GACCCAAGGG AATGGCAAAA CAGTTCTTCC ATCCTCTGTT
    TATAGAAATG GAACCGGCAT CATCTATCAT TATCATGGAA TGGTTTCCCA GAAGCTGGAG
    GGAGACAGTT CTCCATCTCG CAAAAGGAAG GCCAAAGTAG CCATCACCAG TCTGCCACAA
    AAGACAAGCC AAGTGGCAGC GCTGCCTCAA GCAGATAAGA TGGAATCCAC AAGTGACTCA
    CACTTCTCCA GACAGGACCG ATTGCCCTCA ATTCCAAAGG ACTGCACACT GGAATTAAAG
    GGACTTCTCC ACTTCCACGA AAGCCTCCGG AAGCTGTACA AGTGTGATGG AATTACCTGG
    AAAGCCTGGA GCCCCCAAAC CGAGGAGGTG GAAGGTGAAG CCTGCCCAGC TGGGTGGCAC
    CATCATTCAG GCTACTGCCA CTCTTTGGTC ACAGAGCAGA AGGGCACCTG GAGCACTGCT
    GCCCGAGCTT GCAAGGAACA AATAAAACTG TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.