LOC481228 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC481228
Entrez Gene ID	481228
Full Name	sterol regulatory element binding transcription factor 2
Synonyms	CCDC134,SREBP2
General protein information	Preferred Names sterol regulatory element binding transcription factor 2 Names sterol regulatory element-binding protein 2 coiled-coil domain-containing protein 134 coiled-coil domain containing 134
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014117321.1	XP_013972796.1	sterol regulatory element-binding protein 2 isoform X2
XM_003431492.4	XP_003431540.1	coiled-coil domain-containing protein 134 isoform X5
XM_022424366.1	XP_022280074.1	sterol regulatory element-binding protein 2 isoform X1
XM_022424368.1	XP_022280076.1	sterol regulatory element-binding protein 2 isoform X4
XM_843994.4	XP_849087.3	sterol regulatory element-binding protein 2 isoform X1
XM_843994.5	XP_849087.3	sterol regulatory element-binding protein 2 isoform X2
XM_022424367.1	XP_022280075.1	sterol regulatory element-binding protein 2 isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SREBF2	XP_849087.3
Danio rerio (zebrafish)	srebf2	NP_001082935.1
	SREBF2	XP_001101257.2
Homo sapiens (human)	SREBF2	NP_004590.2
Pan troglodytes (chimpanzee)	SREBF2	XP_003317335.1
Rattus norvegicus (Norway rat)	Srebf2	NP_001028866.1
Gallus gallus (chicken)	SREBF2	XP_004937758.1
Xenopus tropicalis (tropical clawed frog)	srebf2	NP_001116910.2
Bos taurus (cattle)	SREBF2	NP_001192529.1
Mus musculus (house mouse)	Srebf2	NP_150087.1

Gene Ontology

The following LOC481228 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC481228 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe13407	XM_003431492.4 Latest version!	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OCe13406	XM_843994.5 Latest version!	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe291413	XM_022424366.1 Latest version!	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe291414	XM_022424368.1 Latest version!	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe38886	XM_022424367.1 Latest version!	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe38886	XM_014117321.1 Latest version!	Canis lupus familiaris sterol regulatory element binding transcription factor 2 (SREBF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OCe13406	XM_843994.4	Canis lupus familiaris sterol regulatory element binding transcription factor 2 (SREBF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC481228 ( XM_003431492.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003431540.1 Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe13407
Clone ID Related Accession (Same CDS sequence)	XM_003431492.4
Accession Version	XM_003431492.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	coiled-coil domain-containing protein 134 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003431492.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTTC TTCAGTTCCT GGCCTTCTTC TTCATCCTGC TATTGTCTGG GACAGGAGTC 
ACAGGCGCCC TGAAAACCTC CCTGGACCCT AGTCTGGAAA TCTACAAGAA GATGTTTGAG 
GTGAAACGAC GAGAACAGCT GTTGGCACTG AAAAACCTGG CACAGCTAAA CGATGTTCAC 
CAGCAGTACA AGATTCTTGA TGTCATGCTC AAGGGGCTCT TTAAGGTGCT GGAGGACTCA 
CGGACAGTGC TCATTGCTGC AGATGTGCTC CCAGATGGGC CCTTCCCCCA GGACGAGAAA 
CTGAAGGATG CTTTCTCCCA AGTAGTGGAG AACACAGCCT TCTTCGGTGA TGTGGTGCTG 
CGCTTCCCGA GGATTGTGCA CCACTACTTT GACCACAATT CCAACTGGAA CCTCCTTATC 
CGCTGGGGCA TCAGCTTCTG CAACCAGTCG GGCGTGTTTG ACCAGGGGCC CCACTCGCCC 
ATCCTTAGCC TGATGGCCCA GGAGCTGGGG ATCAGTGAGA AAGACTCTGA CTTCCAGAAC 
CCATTTAAAG TAGACCGCCC AGAGTTCATT TCCAGCACCG ACCCTTTCCA GAAAGCCCTG 
AGGGAGGAGG AGAAACGCAG GAAGAAAGAG GAGAAGCGGA AAGAGATCCG AAAAGGCCCA 
CGGATCTCAA GATCCCAGTC TGAGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431540.1
CDS	165..854
Translation	MDLLQFLAFFFILLLSGTGVTGALKTSLDPSLEIYKKMFEVKRREQLLALKNLAQLNDVHQQYKILDVMLKGLFKVLEDSRTVLIAADVLPDGPFPQDEKLKDAFSQVVENTAFFGDVVLRFPRIVHHYFDHNSNWNLLIRWGISFCNQSGVFDQGPHSPILSLMAQELGISEKDSDFQNPFKVDRPEFISSTDPFQKALREEEKRRKKEEKRKEIRKGPRISRSQSEL

Target ORF information:

RefSeq Version	XM_003431492.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431492.4

ORF Insert Sequence:

ATGGATCTTC TTCAGTTCCT GGCCTTCTTC TTCATCCTGC TATTGTCTGG GACAGGAGTC 
ACAGGCGCCC TGAAAACCTC CCTGGACCCT AGTCTGGAAA TCTACAAGAA GATGTTTGAG 
GTGAAACGAC GAGAACAGCT GTTGGCACTG AAAAACCTGG CACAGCTAAA CGATGTTCAC 
CAGCAGTACA AGATTCTTGA TGTCATGCTC AAGGGGCTCT TTAAGGTGCT GGAGGACTCA 
CGGACAGTGC TCATTGCTGC AGATGTGCTC CCAGATGGGC CCTTCCCCCA GGACGAGAAA 
CTGAAGGATG CTTTCTCCCA AGTAGTGGAG AACACAGCCT TCTTCGGTGA TGTGGTGCTG 
CGCTTCCCGA GGATTGTGCA CCACTACTTT GACCACAATT CCAACTGGAA CCTCCTTATC 
CGCTGGGGCA TCAGCTTCTG CAACCAGTCG GGCGTGTTTG ACCAGGGGCC CCACTCGCCC 
ATCCTTAGCC TGATGGCCCA GGAGCTGGGG ATCAGTGAGA AAGACTCTGA CTTCCAGAAC 
CCATTTAAAG TAGACCGCCC AGAGTTCATT TCCAGCACCG ACCCTTTCCA GAAAGCCCTG 
AGGGAGGAGG AGAAACGCAG GAAGAAAGAG GAGAAGCGGA AAGAGATCCG AAAAGGCCCA 
CGGATCTCAA GATCCCAGTC TGAGTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC481228 ( XM_843994.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849087.3 Canis lupus familiaris sterol regulatory element binding transcription factor 2 (SREBF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe13406
Clone ID Related Accession (Same CDS sequence)	XM_843994.4 , XM_843994.5
Accession Version	XM_843994.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	sterol regulatory element-binding protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_843994.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGA GCGGCGAGCT GGGCGATCTG GAGACCATGG AGACGCTCAC GGAGCTGGGC 
GATGAGCTGA CCCTGGGGGA CATCGACGAG ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA 
GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC 
GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG GGCGGAGTGG GGGACCCCGC AATGTCGCGG 
TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC 
GCCCTGCAGG TCAAGGCTCC CCCCACCACG GTCCCTACCC CGCCGCGGGC CACGCCTGTT 
CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG CCCCCGGCCC AGCTGCCGCA GCAGACGGTG 
ATGATCACCC CCACGTTCAG CACCGCTCCG CAGACGAGGA TCATCCAGCA GCCTTTGATA 
TACCAGAATG CAGCCACCAG CTTTCAAGTC CTTCAGCCTC AAGTCCAAAG CCTAGTGACA 
TCCTCCCAGG TGCAGCCAGT CACCATCCAG CAGCAGGTGC AGACGGTACA GGCCCAAAGG 
GTGCTGACAC AGACAGCCAA CGGCACGCTG CAGACCCTCG CCCCGGCCAC GGTACAGACA 
GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG GTCCTGGTAC AGCCTCAGAT CATCAAGACA 
GATTCCCTTG TTTTGACCAC CTTAAAGACA GATGGCAGCC CTGTCATGGC CGCAGTCCAG 
AACCCAGCCC TCACCGCCCT CACCACCCCC ATCCAGACAG CTGCCCTTCA AGTACCAACT 
CTTGTGGGCA GCAATGGAAC CATTTTGACC ACAATGCCTG TGATGATGGG GCAAGAGAAA 
GTGCCCATCA AGCAGGTGCC TGGGGGAGTC AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG 
AGGCGGACGA CACACAACAT CATTGAGAAG CGGTATCGTT CCTCTATTAA TGACAAAATC 
ATTGAGTTGA AGGACCTGGT CATGGGGACA GATGCCAAGA TGCACAAGTC TGGTGTTCTG 
AGGAAGGCCA TTGACTACAT CAAGTACTTG CAGCAAGTCA ATCATAAACT ACGCCAGGAG 
AACATGGTGC TGAAGCTGGC AAATCAGAAA AACAAGCTCC TGAAGGGCAT CGACCTAGGC 
AGCCTGGTGG ACAATGATGT GGAGCTAAAG ATTGAAGACT TCAATCAGAA TATCCTTCTG 
ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC CAGGCTGGTT TCTCCCCCTA CTCCATCGAC 
TCCGAGCCAG GAAGCCCTCT GTTGGATGAT GCAAAGGTCA AAGATGAGCC AGACTCTCCT 
CCCGTGGCGC TGGGCATGGT GGACCGCTCT CGGATTCTGC TCTGTGTGCT CACCTTCCTC 
TGCCTCTCCT TTAACCCCCT GACTGCCCTG TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC 
CAGCACCCAT ATTCCGGCTC TGGCCGCAGC ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC 
TGGTTTGATT GGATGATGCC AACGCTTCTC TTATGGCTGA TAAATGGTGT GATTGTCCTG 
AGCGTCTTTG TGAAGCTGCT GGTCCATGGA GAGCCAGTGA TCCGGCCACA TTCACGCTCC 
TCAGTCACCT TCTGGAGACA CCGGAAGCAG GCCGACCTGG ACCTCGCCAG GGGGGATTTT 
GCAGCTGCTG CAGCCAACCT GCAAACCTGC CTGTCGGTGT TGGGCCGCAC ACTGCCCACC 
TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC TGGAACGTGA TCCGCTACAG CCTGCAGAAG 
CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC 
ACCACCGCAG GCTTTGAGGA CGAGGCGAAG GCCAGCGCCC GGGACGCGGC CCTGGCCTAT 
CACAGGCTGC ACCAGCTGCA CATCACTGGG AAGCTTCCTG CGGGATCCGC CTGTTCAGAT 
GTACACATGG CTTTGTGCGC CGTGAACCTG GCGGAATGTG CAGAGGAGAA AATCCCACCG 
AGCACGTTGG TTGAGATCCA TCTGACTGCC GCCATGGGGC TCAAGACCCA GTGTGGAGGC 
AAGCTTGGCT TCCTGGCGAG CTACTTCCTC AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG 
CGCAGCGCCG TCCCTGACTC ACTGCGCTGG CTGTGCCACC CCCTGGGCCA GAAGTTTTTC 
ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT GCCAAGGAGA GTCTCTACTG TGCTCAGAGG 
AACCCAGCTG ACCCCATCGC ACAGGTCCAC CAGGCCTTCT GCAAGAACCT ACTGGAGCGA 
GCAGTTGAAT CACTGGTGAA GCCTCAGGCC AAGAAGAAGG CTGGAGACCA GGAAGAAGAG 
AGCTGCGAAT TCTCTAATGC TCTGGAGTAC CTGAAATTAC TGAATTCTTT TGTGGACTCT 
GTGGGGATTG TGACCCCGCC CTTCTCCAGC AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA 
GATGTCATCT GTCGGTGGTG GACATCCGCC ATCACTGTGG CCATCAGCTG GCTCCAGGGA 
GACGACGCAG CTGTACGCAC TCATTTTACC GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA 
GTAACAGAGA GCCCCCTGGT GAAGGCCGTC TTCCACACCT GCAGAGCCAT GCACACCTCA 
CTCTTGGGGA AGGCGGATGG GCAGCAGAGT TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC 
CACCTGTGGA GCAGCCTCAA CGTTAGCGGG GCCACCTGCG ACCTCACCCT CAACCACGTG 
GTCCAGCTCC TGACCTGTGA CCTGCTACTG TCACTGAGGA CAGCACTTTG GCAAAAGCAG 
GCAGGCGCCA GCCAGGCTCT GGGCGAGACG TACCACGCAT CAGGTGCTGA ACTGGCTGGC 
TTCCAGCGGG ACCTGGGCAG CCTACGCAGG CTGGCTCACG GCTTCCGCCC AGCATACCGC 
AAGGTGTTCC TGCATGAAGC CACTGTGCGC CTGATGGCAG GAGCCAGCCC CACCCGCACC 
CACCAGCTAC TGGAGCATAG TCTGCGCCGG CGGACTGCTC AGAGCACCAA GCACGGAGAG 
GTGGACACCT GGCCCGGACA GCGAGAGCGG GCCACCGCCA TCCTGCTGGC CTGCCGCCAC 
CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC 
CGCACCCTGG AGAAGGCGGG TGACCGACGC TCCTGCAACG ACTGCCAGCA GATGATTGTC 
AAGTTGGGGG GCGGCACGGC CATCGCCGCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849087.3
CDS	178..3573
Translation	MDESGELGDLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPSGGGGGGGNGSGSAGGGVGDPAMSRSFSQVPLPAFSPAAASPQAPALQVKAPPTTVPTPPRATPVLQPRPQPPAPPPAQLPQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNDVELKIEDFNQNILLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTALLQWGGAHSSDQHPYSGSGRSILSLESGSGGWFDWMMPTLLLWLINGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAANLQTCLSVLGRTLPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQHRRAAPATTAGFEDEAKASARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTQCGGKLGFLASYFLSRAQSLCGPERSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSNALEYLKLLNSFVDSVGIVTPPFSSSSVLKSALGPDVICRWWTSAITVAISWLQGDDAAVRTHFTEVERVPKALEVTESPLVKAVFHTCRAMHTSLLGKADGQQSSFCHCERASGHLWSSLNVSGATCDLTLNHVVQLLTCDLLLSLRTALWQKQAGASQALGETYHASGAELAGFQRDLGSLRRLAHGFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTAQSTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKAGDRRSCNDCQQMIVKLGGGTAIAAS

Target ORF information:

RefSeq Version	XM_843994.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sterol regulatory element binding transcription factor 2 (SREBF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_843994.4

ORF Insert Sequence:

ATGGACGAGA GCGGCGAGCT GGGCGATCTG GAGACCATGG AGACGCTCAC GGAGCTGGGC 
GATGAGCTGA CCCTGGGGGA CATCGACGAG ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA 
GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC 
GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG GGCGGAGTGG GGGACCCCGC AATGTCGCGG 
TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC 
GCCCTGCAGG TCAAGGCTCC CCCCACCACG GTCCCTACCC CGCCGCGGGC CACGCCTGTT 
CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG CCCCCGGCCC AGCTGCCGCA GCAGACGGTG 
ATGATCACCC CCACGTTCAG CACCGCTCCG CAGACGAGGA TCATCCAGCA GCCTTTGATA 
TACCAGAATG CAGCCACCAG CTTTCAAGTC CTTCAGCCTC AAGTCCAAAG CCTAGTGACA 
TCCTCCCAGG TGCAGCCAGT CACCATCCAG CAGCAGGTGC AGACGGTACA GGCCCAAAGG 
GTGCTGACAC AGACAGCCAA CGGCACGCTG CAGACCCTCG CCCCGGCCAC GGTACAGACA 
GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG GTCCTGGTAC AGCCTCAGAT CATCAAGACA 
GATTCCCTTG TTTTGACCAC CTTAAAGACA GATGGCAGCC CTGTCATGGC CGCAGTCCAG 
AACCCAGCCC TCACCGCCCT CACCACCCCC ATCCAGACAG CTGCCCTTCA AGTACCAACT 
CTTGTGGGCA GCAATGGAAC CATTTTGACC ACAATGCCTG TGATGATGGG GCAAGAGAAA 
GTGCCCATCA AGCAGGTGCC TGGGGGAGTC AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG 
AGGCGGACGA CACACAACAT CATTGAGAAG CGGTATCGTT CCTCTATTAA TGACAAAATC 
ATTGAGTTGA AGGACCTGGT CATGGGGACA GATGCCAAGA TGCACAAGTC TGGTGTTCTG 
AGGAAGGCCA TTGACTACAT CAAGTACTTG CAGCAAGTCA ATCATAAACT ACGCCAGGAG 
AACATGGTGC TGAAGCTGGC AAATCAGAAA AACAAGCTCC TGAAGGGCAT CGACCTAGGC 
AGCCTGGTGG ACAATGATGT GGAGCTAAAG ATTGAAGACT TCAATCAGAA TATCCTTCTG 
ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC CAGGCTGGTT TCTCCCCCTA CTCCATCGAC 
TCCGAGCCAG GAAGCCCTCT GTTGGATGAT GCAAAGGTCA AAGATGAGCC AGACTCTCCT 
CCCGTGGCGC TGGGCATGGT GGACCGCTCT CGGATTCTGC TCTGTGTGCT CACCTTCCTC 
TGCCTCTCCT TTAACCCCCT GACTGCCCTG TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC 
CAGCACCCAT ATTCCGGCTC TGGCCGCAGC ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC 
TGGTTTGATT GGATGATGCC AACGCTTCTC TTATGGCTGA TAAATGGTGT GATTGTCCTG 
AGCGTCTTTG TGAAGCTGCT GGTCCATGGA GAGCCAGTGA TCCGGCCACA TTCACGCTCC 
TCAGTCACCT TCTGGAGACA CCGGAAGCAG GCCGACCTGG ACCTCGCCAG GGGGGATTTT 
GCAGCTGCTG CAGCCAACCT GCAAACCTGC CTGTCGGTGT TGGGCCGCAC ACTGCCCACC 
TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC TGGAACGTGA TCCGCTACAG CCTGCAGAAG 
CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC 
ACCACCGCAG GCTTTGAGGA CGAGGCGAAG GCCAGCGCCC GGGACGCGGC CCTGGCCTAT 
CACAGGCTGC ACCAGCTGCA CATCACTGGG AAGCTTCCTG CGGGATCCGC CTGTTCAGAT 
GTACACATGG CTTTGTGCGC CGTGAACCTG GCGGAATGTG CAGAGGAGAA AATCCCACCG 
AGCACGTTGG TTGAGATCCA TCTGACTGCC GCCATGGGGC TCAAGACCCA GTGTGGAGGC 
AAGCTTGGCT TCCTGGCGAG CTACTTCCTC AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG 
CGCAGCGCCG TCCCTGACTC ACTGCGCTGG CTGTGCCACC CCCTGGGCCA GAAGTTTTTC 
ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT GCCAAGGAGA GTCTCTACTG TGCTCAGAGG 
AACCCAGCTG ACCCCATCGC ACAGGTCCAC CAGGCCTTCT GCAAGAACCT ACTGGAGCGA 
GCAGTTGAAT CACTGGTGAA GCCTCAGGCC AAGAAGAAGG CTGGAGACCA GGAAGAAGAG 
AGCTGCGAAT TCTCTAATGC TCTGGAGTAC CTGAAATTAC TGAATTCTTT TGTGGACTCT 
GTGGGGATTG TGACCCCGCC CTTCTCCAGC AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA 
GATGTCATCT GTCGGTGGTG GACATCCGCC ATCACTGTGG CCATCAGCTG GCTCCAGGGA 
GACGACGCAG CTGTACGCAC TCATTTTACC GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA 
GTAACAGAGA GCCCCCTGGT GAAGGCCGTC TTCCACACCT GCAGAGCCAT GCACACCTCA 
CTCTTGGGGA AGGCGGATGG GCAGCAGAGT TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC 
CACCTGTGGA GCAGCCTCAA CGTTAGCGGG GCCACCTGCG ACCTCACCCT CAACCACGTG 
GTCCAGCTCC TGACCTGTGA CCTGCTACTG TCACTGAGGA CAGCACTTTG GCAAAAGCAG 
GCAGGCGCCA GCCAGGCTCT GGGCGAGACG TACCACGCAT CAGGTGCTGA ACTGGCTGGC 
TTCCAGCGGG ACCTGGGCAG CCTACGCAGG CTGGCTCACG GCTTCCGCCC AGCATACCGC 
AAGGTGTTCC TGCATGAAGC CACTGTGCGC CTGATGGCAG GAGCCAGCCC CACCCGCACC 
CACCAGCTAC TGGAGCATAG TCTGCGCCGG CGGACTGCTC AGAGCACCAA GCACGGAGAG 
GTGGACACCT GGCCCGGACA GCGAGAGCGG GCCACCGCCA TCCTGCTGGC CTGCCGCCAC 
CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC 
CGCACCCTGG AGAAGGCGGG TGACCGACGC TCCTGCAACG ACTGCCAGCA GATGATTGTC 
AAGTTGGGGG GCGGCACGGC CATCGCCGCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC481228 ( XM_843994.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849087.3 Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe13406
Clone ID Related Accession (Same CDS sequence)	XM_843994.4 , XM_843994.5
Accession Version	XM_843994.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sterol regulatory element-binding protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843994.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGAGA GCGGCGAGCT GGGCGATCTG GAGACCATGG AGACGCTCAC GGAGCTGGGC 
GATGAGCTGA CCCTGGGGGA CATCGACGAG ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA 
GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC 
GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG GGCGGAGTGG GGGACCCCGC AATGTCGCGG 
TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC 
GCCCTGCAGG TCAAGGCTCC CCCCACCACG GTCCCTACCC CGCCGCGGGC CACGCCTGTT 
CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG CCCCCGGCCC AGCTGCCGCA GCAGACGGTG 
ATGATCACCC CCACGTTCAG CACCGCTCCG CAGACGAGGA TCATCCAGCA GCCTTTGATA 
TACCAGAATG CAGCCACCAG CTTTCAAGTC CTTCAGCCTC AAGTCCAAAG CCTAGTGACA 
TCCTCCCAGG TGCAGCCAGT CACCATCCAG CAGCAGGTGC AGACGGTACA GGCCCAAAGG 
GTGCTGACAC AGACAGCCAA CGGCACGCTG CAGACCCTCG CCCCGGCCAC GGTACAGACA 
GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG GTCCTGGTAC AGCCTCAGAT CATCAAGACA 
GATTCCCTTG TTTTGACCAC CTTAAAGACA GATGGCAGCC CTGTCATGGC CGCAGTCCAG 
AACCCAGCCC TCACCGCCCT CACCACCCCC ATCCAGACAG CTGCCCTTCA AGTACCAACT 
CTTGTGGGCA GCAATGGAAC CATTTTGACC ACAATGCCTG TGATGATGGG GCAAGAGAAA 
GTGCCCATCA AGCAGGTGCC TGGGGGAGTC AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG 
AGGCGGACGA CACACAACAT CATTGAGAAG CGGTATCGTT CCTCTATTAA TGACAAAATC 
ATTGAGTTGA AGGACCTGGT CATGGGGACA GATGCCAAGA TGCACAAGTC TGGTGTTCTG 
AGGAAGGCCA TTGACTACAT CAAGTACTTG CAGCAAGTCA ATCATAAACT ACGCCAGGAG 
AACATGGTGC TGAAGCTGGC AAATCAGAAA AACAAGCTCC TGAAGGGCAT CGACCTAGGC 
AGCCTGGTGG ACAATGATGT GGAGCTAAAG ATTGAAGACT TCAATCAGAA TATCCTTCTG 
ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC CAGGCTGGTT TCTCCCCCTA CTCCATCGAC 
TCCGAGCCAG GAAGCCCTCT GTTGGATGAT GCAAAGGTCA AAGATGAGCC AGACTCTCCT 
CCCGTGGCGC TGGGCATGGT GGACCGCTCT CGGATTCTGC TCTGTGTGCT CACCTTCCTC 
TGCCTCTCCT TTAACCCCCT GACTGCCCTG TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC 
CAGCACCCAT ATTCCGGCTC TGGCCGCAGC ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC 
TGGTTTGATT GGATGATGCC AACGCTTCTC TTATGGCTGA TAAATGGTGT GATTGTCCTG 
AGCGTCTTTG TGAAGCTGCT GGTCCATGGA GAGCCAGTGA TCCGGCCACA TTCACGCTCC 
TCAGTCACCT TCTGGAGACA CCGGAAGCAG GCCGACCTGG ACCTCGCCAG GGGGGATTTT 
GCAGCTGCTG CAGCCAACCT GCAAACCTGC CTGTCGGTGT TGGGCCGCAC ACTGCCCACC 
TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC TGGAACGTGA TCCGCTACAG CCTGCAGAAG 
CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC 
ACCACCGCAG GCTTTGAGGA CGAGGCGAAG GCCAGCGCCC GGGACGCGGC CCTGGCCTAT 
CACAGGCTGC ACCAGCTGCA CATCACTGGG AAGCTTCCTG CGGGATCCGC CTGTTCAGAT 
GTACACATGG CTTTGTGCGC CGTGAACCTG GCGGAATGTG CAGAGGAGAA AATCCCACCG 
AGCACGTTGG TTGAGATCCA TCTGACTGCC GCCATGGGGC TCAAGACCCA GTGTGGAGGC 
AAGCTTGGCT TCCTGGCGAG CTACTTCCTC AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG 
CGCAGCGCCG TCCCTGACTC ACTGCGCTGG CTGTGCCACC CCCTGGGCCA GAAGTTTTTC 
ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT GCCAAGGAGA GTCTCTACTG TGCTCAGAGG 
AACCCAGCTG ACCCCATCGC ACAGGTCCAC CAGGCCTTCT GCAAGAACCT ACTGGAGCGA 
GCAGTTGAAT CACTGGTGAA GCCTCAGGCC AAGAAGAAGG CTGGAGACCA GGAAGAAGAG 
AGCTGCGAAT TCTCTAATGC TCTGGAGTAC CTGAAATTAC TGAATTCTTT TGTGGACTCT 
GTGGGGATTG TGACCCCGCC CTTCTCCAGC AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA 
GATGTCATCT GTCGGTGGTG GACATCCGCC ATCACTGTGG CCATCAGCTG GCTCCAGGGA 
GACGACGCAG CTGTACGCAC TCATTTTACC GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA 
GTAACAGAGA GCCCCCTGGT GAAGGCCGTC TTCCACACCT GCAGAGCCAT GCACACCTCA 
CTCTTGGGGA AGGCGGATGG GCAGCAGAGT TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC 
CACCTGTGGA GCAGCCTCAA CGTTAGCGGG GCCACCTGCG ACCTCACCCT CAACCACGTG 
GTCCAGCTCC TGACCTGTGA CCTGCTACTG TCACTGAGGA CAGCACTTTG GCAAAAGCAG 
GCAGGCGCCA GCCAGGCTCT GGGCGAGACG TACCACGCAT CAGGTGCTGA ACTGGCTGGC 
TTCCAGCGGG ACCTGGGCAG CCTACGCAGG CTGGCTCACG GCTTCCGCCC AGCATACCGC 
AAGGTGTTCC TGCATGAAGC CACTGTGCGC CTGATGGCAG GAGCCAGCCC CACCCGCACC 
CACCAGCTAC TGGAGCATAG TCTGCGCCGG CGGACTGCTC AGAGCACCAA GCACGGAGAG 
GTGGACACCT GGCCCGGACA GCGAGAGCGG GCCACCGCCA TCCTGCTGGC CTGCCGCCAC 
CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC 
CGCACCCTGG AGAAGGCGGG TGACCGACGC TCCTGCAACG ACTGCCAGCA GATGATTGTC 
AAGTTGGGGG GCGGCACGGC CATCGCCGCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849087.3
CDS	381..3776
Translation	MDESGELGDLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPSGGGGGGGNGSGSAGGGVGDPAMSRSFSQVPLPAFSPAAASPQAPALQVKAPPTTVPTPPRATPVLQPRPQPPAPPPAQLPQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNDVELKIEDFNQNILLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTALLQWGGAHSSDQHPYSGSGRSILSLESGSGGWFDWMMPTLLLWLINGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAANLQTCLSVLGRTLPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQHRRAAPATTAGFEDEAKASARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTQCGGKLGFLASYFLSRAQSLCGPERSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSNALEYLKLLNSFVDSVGIVTPPFSSSSVLKSALGPDVICRWWTSAITVAISWLQGDDAAVRTHFTEVERVPKALEVTESPLVKAVFHTCRAMHTSLLGKADGQQSSFCHCERASGHLWSSLNVSGATCDLTLNHVVQLLTCDLLLSLRTALWQKQAGASQALGETYHASGAELAGFQRDLGSLRRLAHGFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTAQSTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKAGDRRSCNDCQQMIVKLGGGTAIAAS

Target ORF information:

RefSeq Version	XM_843994.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_843994.5

ORF Insert Sequence:

ATGGACGAGA GCGGCGAGCT GGGCGATCTG GAGACCATGG AGACGCTCAC GGAGCTGGGC 
GATGAGCTGA CCCTGGGGGA CATCGACGAG ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA 
GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC 
GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG GGCGGAGTGG GGGACCCCGC AATGTCGCGG 
TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC 
GCCCTGCAGG TCAAGGCTCC CCCCACCACG GTCCCTACCC CGCCGCGGGC CACGCCTGTT 
CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG CCCCCGGCCC AGCTGCCGCA GCAGACGGTG 
ATGATCACCC CCACGTTCAG CACCGCTCCG CAGACGAGGA TCATCCAGCA GCCTTTGATA 
TACCAGAATG CAGCCACCAG CTTTCAAGTC CTTCAGCCTC AAGTCCAAAG CCTAGTGACA 
TCCTCCCAGG TGCAGCCAGT CACCATCCAG CAGCAGGTGC AGACGGTACA GGCCCAAAGG 
GTGCTGACAC AGACAGCCAA CGGCACGCTG CAGACCCTCG CCCCGGCCAC GGTACAGACA 
GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG GTCCTGGTAC AGCCTCAGAT CATCAAGACA 
GATTCCCTTG TTTTGACCAC CTTAAAGACA GATGGCAGCC CTGTCATGGC CGCAGTCCAG 
AACCCAGCCC TCACCGCCCT CACCACCCCC ATCCAGACAG CTGCCCTTCA AGTACCAACT 
CTTGTGGGCA GCAATGGAAC CATTTTGACC ACAATGCCTG TGATGATGGG GCAAGAGAAA 
GTGCCCATCA AGCAGGTGCC TGGGGGAGTC AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG 
AGGCGGACGA CACACAACAT CATTGAGAAG CGGTATCGTT CCTCTATTAA TGACAAAATC 
ATTGAGTTGA AGGACCTGGT CATGGGGACA GATGCCAAGA TGCACAAGTC TGGTGTTCTG 
AGGAAGGCCA TTGACTACAT CAAGTACTTG CAGCAAGTCA ATCATAAACT ACGCCAGGAG 
AACATGGTGC TGAAGCTGGC AAATCAGAAA AACAAGCTCC TGAAGGGCAT CGACCTAGGC 
AGCCTGGTGG ACAATGATGT GGAGCTAAAG ATTGAAGACT TCAATCAGAA TATCCTTCTG 
ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC CAGGCTGGTT TCTCCCCCTA CTCCATCGAC 
TCCGAGCCAG GAAGCCCTCT GTTGGATGAT GCAAAGGTCA AAGATGAGCC AGACTCTCCT 
CCCGTGGCGC TGGGCATGGT GGACCGCTCT CGGATTCTGC TCTGTGTGCT CACCTTCCTC 
TGCCTCTCCT TTAACCCCCT GACTGCCCTG TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC 
CAGCACCCAT ATTCCGGCTC TGGCCGCAGC ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC 
TGGTTTGATT GGATGATGCC AACGCTTCTC TTATGGCTGA TAAATGGTGT GATTGTCCTG 
AGCGTCTTTG TGAAGCTGCT GGTCCATGGA GAGCCAGTGA TCCGGCCACA TTCACGCTCC 
TCAGTCACCT TCTGGAGACA CCGGAAGCAG GCCGACCTGG ACCTCGCCAG GGGGGATTTT 
GCAGCTGCTG CAGCCAACCT GCAAACCTGC CTGTCGGTGT TGGGCCGCAC ACTGCCCACC 
TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC TGGAACGTGA TCCGCTACAG CCTGCAGAAG 
CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC 
ACCACCGCAG GCTTTGAGGA CGAGGCGAAG GCCAGCGCCC GGGACGCGGC CCTGGCCTAT 
CACAGGCTGC ACCAGCTGCA CATCACTGGG AAGCTTCCTG CGGGATCCGC CTGTTCAGAT 
GTACACATGG CTTTGTGCGC CGTGAACCTG GCGGAATGTG CAGAGGAGAA AATCCCACCG 
AGCACGTTGG TTGAGATCCA TCTGACTGCC GCCATGGGGC TCAAGACCCA GTGTGGAGGC 
AAGCTTGGCT TCCTGGCGAG CTACTTCCTC AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG 
CGCAGCGCCG TCCCTGACTC ACTGCGCTGG CTGTGCCACC CCCTGGGCCA GAAGTTTTTC 
ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT GCCAAGGAGA GTCTCTACTG TGCTCAGAGG 
AACCCAGCTG ACCCCATCGC ACAGGTCCAC CAGGCCTTCT GCAAGAACCT ACTGGAGCGA 
GCAGTTGAAT CACTGGTGAA GCCTCAGGCC AAGAAGAAGG CTGGAGACCA GGAAGAAGAG 
AGCTGCGAAT TCTCTAATGC TCTGGAGTAC CTGAAATTAC TGAATTCTTT TGTGGACTCT 
GTGGGGATTG TGACCCCGCC CTTCTCCAGC AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA 
GATGTCATCT GTCGGTGGTG GACATCCGCC ATCACTGTGG CCATCAGCTG GCTCCAGGGA 
GACGACGCAG CTGTACGCAC TCATTTTACC GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA 
GTAACAGAGA GCCCCCTGGT GAAGGCCGTC TTCCACACCT GCAGAGCCAT GCACACCTCA 
CTCTTGGGGA AGGCGGATGG GCAGCAGAGT TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC 
CACCTGTGGA GCAGCCTCAA CGTTAGCGGG GCCACCTGCG ACCTCACCCT CAACCACGTG 
GTCCAGCTCC TGACCTGTGA CCTGCTACTG TCACTGAGGA CAGCACTTTG GCAAAAGCAG 
GCAGGCGCCA GCCAGGCTCT GGGCGAGACG TACCACGCAT CAGGTGCTGA ACTGGCTGGC 
TTCCAGCGGG ACCTGGGCAG CCTACGCAGG CTGGCTCACG GCTTCCGCCC AGCATACCGC 
AAGGTGTTCC TGCATGAAGC CACTGTGCGC CTGATGGCAG GAGCCAGCCC CACCCGCACC 
CACCAGCTAC TGGAGCATAG TCTGCGCCGG CGGACTGCTC AGAGCACCAA GCACGGAGAG 
GTGGACACCT GGCCCGGACA GCGAGAGCGG GCCACCGCCA TCCTGCTGGC CTGCCGCCAC 
CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC 
CGCACCCTGG AGAAGGCGGG TGACCGACGC TCCTGCAACG ACTGCCAGCA GATGATTGTC 
AAGTTGGGGG GCGGCACGGC CATCGCCGCC TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC481228 ( XM_022424366.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280074.1 Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X1, mRNA.

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CloneID	OCe291413
Clone ID Related Accession (Same CDS sequence)	XM_022424366.1
Accession Version	XM_022424366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sterol regulatory element-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCTTC TTCAGTTCCT GGCCTTCTTC TTCATCCTGC TATTGTCTGG GACAGGAGTC 
ACAGGCGCCC TGAAAACCTC CCTGGACCCT AGTCTGGAAA TCTACAAGAA GATGTTTGAG 
GTGAAACGAC GAGAACAGCT GTTGGCACTG AAAAACCTGG CACAGCTAAA CGATGTTCAC 
CAGCAGTACA AGATTCTTGA TGTCATGCTC AAGGGGCTCT TTAAGGTGCT GGAGGACTCA 
CGGACAGTGC TCATTGCTGC AGATGTGCTC CCAGATGGGC CCTTCCCCCA GGACGAGAAA 
CTGAAGGATG AGATGCTGCA GTTTGTCAGC AATCAAGTTG GAGAGTTCCC TGACTTGTTC 
TCCGAGCAGC TGTGTAGCTC CTTCCCCAGC GGCGGCGGCG GCGGCGGCGG CAATGGCAGC 
GGCAGTGCTG GGGGCGGAGT GGGGGACCCC GCAATGTCGC GGTCCTTCAG CCAGGTCCCG 
CTACCTGCCT TCTCTCCCGC CGCCGCCTCC CCGCAGGCTC CCGCCCTGCA GGTCAAGGCT 
CCCCCCACCA CGGTCCCTAC CCCGCCGCGG GCCACGCCTG TTCTTCAGCC CCGCCCCCAG 
CCCCCGGCCC CGCCCCCGGC CCAGCTGCCG CAGCAGACGG TGATGATCAC CCCCACGTTC 
AGCACCGCTC CGCAGACGAG GATCATCCAG CAGCCTTTGA TATACCAGAA TGCAGCCACC 
AGCTTTCAAG TCCTTCAGCC TCAAGTCCAA AGCCTAGTGA CATCCTCCCA GGTGCAGCCA 
GTCACCATCC AGCAGCAGGT GCAGACGGTA CAGGCCCAAA GGGTGCTGAC ACAGACAGCC 
AACGGCACGC TGCAGACCCT CGCCCCGGCC ACGGTACAGA CAGTTGCTGC ACCCCAGGTG 
CAACAGGTCC CGGTCCTGGT ACAGCCTCAG ATCATCAAGA CAGATTCCCT TGTTTTGACC 
ACCTTAAAGA CAGATGGCAG CCCTGTCATG GCCGCAGTCC AGAACCCAGC CCTCACCGCC 
CTCACCACCC CCATCCAGAC AGCTGCCCTT CAAGTACCAA CTCTTGTGGG CAGCAATGGA 
ACCATTTTGA CCACAATGCC TGTGATGATG GGGCAAGAGA AAGTGCCCAT CAAGCAGGTG 
CCTGGGGGAG TCAAGCAGCT GGAGCCCCCC AAAGAAGGAG AGAGGCGGAC GACACACAAC 
ATCATTGAGA AGCGGTATCG TTCCTCTATT AATGACAAAA TCATTGAGTT GAAGGACCTG 
GTCATGGGGA CAGATGCCAA GATGCACAAG TCTGGTGTTC TGAGGAAGGC CATTGACTAC 
ATCAAGTACT TGCAGCAAGT CAATCATAAA CTACGCCAGG AGAACATGGT GCTGAAGCTG 
GCAAATCAGA AAAACAAGCT CCTGAAGGGC ATCGACCTAG GCAGCCTGGT GGACAATGAT 
GTGGAGCTAA AGATTGAAGA CTTCAATCAG AATATCCTTC TGATGTCCCC TCCGGCCTCT 
GACTCAGGGT CCCAGGCTGG TTTCTCCCCC TACTCCATCG ACTCCGAGCC AGGAAGCCCT 
CTGTTGGATG ATGCAAAGGT CAAAGATGAG CCAGACTCTC CTCCCGTGGC GCTGGGCATG 
GTGGACCGCT CTCGGATTCT GCTCTGTGTG CTCACCTTCC TCTGCCTCTC CTTTAACCCC 
CTGACTGCCC TGTTGCAGTG GGGAGGGGCC CACAGCTCTG ACCAGCACCC ATATTCCGGC 
TCTGGCCGCA GCATCCTGTC ACTTGAGTCA GGTTCTGGGG GCTGGTTTGA TTGGATGATG 
CCAACGCTTC TCTTATGGCT GATAAATGGT GTGATTGTCC TGAGCGTCTT TGTGAAGCTG 
CTGGTCCATG GAGAGCCAGT GATCCGGCCA CATTCACGCT CCTCAGTCAC CTTCTGGAGA 
CACCGGAAGC AGGCCGACCT GGACCTCGCC AGGGGGGATT TTGCAGCTGC TGCAGCCAAC 
CTGCAAACCT GCCTGTCGGT GTTGGGCCGC ACACTGCCCA CCTCCCGCCT GGACCTGGCC 
TGCAGCCTCT CCTGGAACGT GATCCGCTAC AGCCTGCAGA AGCTGCGCCT GGTGCGCTGG 
CTGCTCAAGA AGGTCTTCCA GCACCGGCGG GCTGCCCCGG CCACCACCGC AGGCTTTGAG 
GACGAGGCGA AGGCCAGCGC CCGGGACGCG GCCCTGGCCT ATCACAGGCT GCACCAGCTG 
CACATCACTG GGAAGCTTCC TGCGGGATCC GCCTGTTCAG ATGTACACAT GGCTTTGTGC 
GCCGTGAACC TGGCGGAATG TGCAGAGGAG AAAATCCCAC CGAGCACGTT GGTTGAGATC 
CATCTGACTG CCGCCATGGG GCTCAAGACC CAGTGTGGAG GCAAGCTTGG CTTCCTGGCG 
AGCTACTTCC TCAGTAGAGC CCAGAGCCTG TGTGGCCCGG AGCGCAGCGC CGTCCCTGAC 
TCACTGCGCT GGCTGTGCCA CCCCCTGGGC CAGAAGTTTT TCATGGAGCG GAGCTGGTCG 
GTGAAGTCAG CTGCCAAGGA GAGTCTCTAC TGTGCTCAGA GGAACCCAGC TGACCCCATC 
GCACAGGTCC ACCAGGCCTT CTGCAAGAAC CTACTGGAGC GAGCAGTTGA ATCACTGGTG 
AAGCCTCAGG CCAAGAAGAA GGCTGGAGAC CAGGAAGAAG AGAGCTGCGA ATTCTCTAAT 
GCTCTGGAGT ACCTGAAATT ACTGAATTCT TTTGTGGACT CTGTGGGGAT TGTGACCCCG 
CCCTTCTCCA GCAGCTCCGT GCTCAAGTCG GCCCTTGGTC CAGATGTCAT CTGTCGGTGG 
TGGACATCCG CCATCACTGT GGCCATCAGC TGGCTCCAGG GAGACGACGC AGCTGTACGC 
ACTCATTTTA CCGAAGTAGA GCGTGTCCCC AAAGCCCTGG AAGTAACAGA GAGCCCCCTG 
GTGAAGGCCG TCTTCCACAC CTGCAGAGCC ATGCACACCT CACTCTTGGG GAAGGCGGAT 
GGGCAGCAGA GTTCCTTCTG CCACTGTGAG AGGGCCAGCG GCCACCTGTG GAGCAGCCTC 
AACGTTAGCG GGGCCACCTG CGACCTCACC CTCAACCACG TGGTCCAGCT CCTGACCTGT 
GACCTGCTAC TGTCACTGAG GACAGCACTT TGGCAAAAGC AGGCAGGCGC CAGCCAGGCT 
CTGGGCGAGA CGTACCACGC ATCAGGTGCT GAACTGGCTG GCTTCCAGCG GGACCTGGGC 
AGCCTACGCA GGCTGGCTCA CGGCTTCCGC CCAGCATACC GCAAGGTGTT CCTGCATGAA 
GCCACTGTGC GCCTGATGGC AGGAGCCAGC CCCACCCGCA CCCACCAGCT ACTGGAGCAT 
AGTCTGCGCC GGCGGACTGC TCAGAGCACC AAGCACGGAG AGGTGGACAC CTGGCCCGGA 
CAGCGAGAGC GGGCCACCGC CATCCTGCTG GCCTGCCGCC ACCTTCCCCT GTCCTTCCTG 
TCCTCCCCCG GCCAGCGGGC GGTGCTGCTG GCTGAGGCGG CCCGCACCCT GGAGAAGGCG 
GGTGACCGAC GCTCCTGCAA CGACTGCCAG CAGATGATTG TCAAGTTGGG GGGCGGCACG 
GCCATCGCCG CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280074.1
CDS	151..3768
Translation	MDLLQFLAFFFILLLSGTGVTGALKTSLDPSLEIYKKMFEVKRREQLLALKNLAQLNDVHQQYKILDVMLKGLFKVLEDSRTVLIAADVLPDGPFPQDEKLKDEMLQFVSNQVGEFPDLFSEQLCSSFPSGGGGGGGNGSGSAGGGVGDPAMSRSFSQVPLPAFSPAAASPQAPALQVKAPPTTVPTPPRATPVLQPRPQPPAPPPAQLPQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNDVELKIEDFNQNILLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTALLQWGGAHSSDQHPYSGSGRSILSLESGSGGWFDWMMPTLLLWLINGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAANLQTCLSVLGRTLPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQHRRAAPATTAGFEDEAKASARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTQCGGKLGFLASYFLSRAQSLCGPERSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSNALEYLKLLNSFVDSVGIVTPPFSSSSVLKSALGPDVICRWWTSAITVAISWLQGDDAAVRTHFTEVERVPKALEVTESPLVKAVFHTCRAMHTSLLGKADGQQSSFCHCERASGHLWSSLNVSGATCDLTLNHVVQLLTCDLLLSLRTALWQKQAGASQALGETYHASGAELAGFQRDLGSLRRLAHGFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTAQSTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKAGDRRSCNDCQQMIVKLGGGTAIAAS

Target ORF information:

RefSeq Version	XM_022424366.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424366.1

ORF Insert Sequence:

ATGGATCTTC TTCAGTTCCT GGCCTTCTTC TTCATCCTGC TATTGTCTGG GACAGGAGTC 
ACAGGCGCCC TGAAAACCTC CCTGGACCCT AGTCTGGAAA TCTACAAGAA GATGTTTGAG 
GTGAAACGAC GAGAACAGCT GTTGGCACTG AAAAACCTGG CACAGCTAAA CGATGTTCAC 
CAGCAGTACA AGATTCTTGA TGTCATGCTC AAGGGGCTCT TTAAGGTGCT GGAGGACTCA 
CGGACAGTGC TCATTGCTGC AGATGTGCTC CCAGATGGGC CCTTCCCCCA GGACGAGAAA 
CTGAAGGATG AGATGCTGCA GTTTGTCAGC AATCAAGTTG GAGAGTTCCC TGACTTGTTC 
TCCGAGCAGC TGTGTAGCTC CTTCCCCAGC GGCGGCGGCG GCGGCGGCGG CAATGGCAGC 
GGCAGTGCTG GGGGCGGAGT GGGGGACCCC GCAATGTCGC GGTCCTTCAG CCAGGTCCCG 
CTACCTGCCT TCTCTCCCGC CGCCGCCTCC CCGCAGGCTC CCGCCCTGCA GGTCAAGGCT 
CCCCCCACCA CGGTCCCTAC CCCGCCGCGG GCCACGCCTG TTCTTCAGCC CCGCCCCCAG 
CCCCCGGCCC CGCCCCCGGC CCAGCTGCCG CAGCAGACGG TGATGATCAC CCCCACGTTC 
AGCACCGCTC CGCAGACGAG GATCATCCAG CAGCCTTTGA TATACCAGAA TGCAGCCACC 
AGCTTTCAAG TCCTTCAGCC TCAAGTCCAA AGCCTAGTGA CATCCTCCCA GGTGCAGCCA 
GTCACCATCC AGCAGCAGGT GCAGACGGTA CAGGCCCAAA GGGTGCTGAC ACAGACAGCC 
AACGGCACGC TGCAGACCCT CGCCCCGGCC ACGGTACAGA CAGTTGCTGC ACCCCAGGTG 
CAACAGGTCC CGGTCCTGGT ACAGCCTCAG ATCATCAAGA CAGATTCCCT TGTTTTGACC 
ACCTTAAAGA CAGATGGCAG CCCTGTCATG GCCGCAGTCC AGAACCCAGC CCTCACCGCC 
CTCACCACCC CCATCCAGAC AGCTGCCCTT CAAGTACCAA CTCTTGTGGG CAGCAATGGA 
ACCATTTTGA CCACAATGCC TGTGATGATG GGGCAAGAGA AAGTGCCCAT CAAGCAGGTG 
CCTGGGGGAG TCAAGCAGCT GGAGCCCCCC AAAGAAGGAG AGAGGCGGAC GACACACAAC 
ATCATTGAGA AGCGGTATCG TTCCTCTATT AATGACAAAA TCATTGAGTT GAAGGACCTG 
GTCATGGGGA CAGATGCCAA GATGCACAAG TCTGGTGTTC TGAGGAAGGC CATTGACTAC 
ATCAAGTACT TGCAGCAAGT CAATCATAAA CTACGCCAGG AGAACATGGT GCTGAAGCTG 
GCAAATCAGA AAAACAAGCT CCTGAAGGGC ATCGACCTAG GCAGCCTGGT GGACAATGAT 
GTGGAGCTAA AGATTGAAGA CTTCAATCAG AATATCCTTC TGATGTCCCC TCCGGCCTCT 
GACTCAGGGT CCCAGGCTGG TTTCTCCCCC TACTCCATCG ACTCCGAGCC AGGAAGCCCT 
CTGTTGGATG ATGCAAAGGT CAAAGATGAG CCAGACTCTC CTCCCGTGGC GCTGGGCATG 
GTGGACCGCT CTCGGATTCT GCTCTGTGTG CTCACCTTCC TCTGCCTCTC CTTTAACCCC 
CTGACTGCCC TGTTGCAGTG GGGAGGGGCC CACAGCTCTG ACCAGCACCC ATATTCCGGC 
TCTGGCCGCA GCATCCTGTC ACTTGAGTCA GGTTCTGGGG GCTGGTTTGA TTGGATGATG 
CCAACGCTTC TCTTATGGCT GATAAATGGT GTGATTGTCC TGAGCGTCTT TGTGAAGCTG 
CTGGTCCATG GAGAGCCAGT GATCCGGCCA CATTCACGCT CCTCAGTCAC CTTCTGGAGA 
CACCGGAAGC AGGCCGACCT GGACCTCGCC AGGGGGGATT TTGCAGCTGC TGCAGCCAAC 
CTGCAAACCT GCCTGTCGGT GTTGGGCCGC ACACTGCCCA CCTCCCGCCT GGACCTGGCC 
TGCAGCCTCT CCTGGAACGT GATCCGCTAC AGCCTGCAGA AGCTGCGCCT GGTGCGCTGG 
CTGCTCAAGA AGGTCTTCCA GCACCGGCGG GCTGCCCCGG CCACCACCGC AGGCTTTGAG 
GACGAGGCGA AGGCCAGCGC CCGGGACGCG GCCCTGGCCT ATCACAGGCT GCACCAGCTG 
CACATCACTG GGAAGCTTCC TGCGGGATCC GCCTGTTCAG ATGTACACAT GGCTTTGTGC 
GCCGTGAACC TGGCGGAATG TGCAGAGGAG AAAATCCCAC CGAGCACGTT GGTTGAGATC 
CATCTGACTG CCGCCATGGG GCTCAAGACC CAGTGTGGAG GCAAGCTTGG CTTCCTGGCG 
AGCTACTTCC TCAGTAGAGC CCAGAGCCTG TGTGGCCCGG AGCGCAGCGC CGTCCCTGAC 
TCACTGCGCT GGCTGTGCCA CCCCCTGGGC CAGAAGTTTT TCATGGAGCG GAGCTGGTCG 
GTGAAGTCAG CTGCCAAGGA GAGTCTCTAC TGTGCTCAGA GGAACCCAGC TGACCCCATC 
GCACAGGTCC ACCAGGCCTT CTGCAAGAAC CTACTGGAGC GAGCAGTTGA ATCACTGGTG 
AAGCCTCAGG CCAAGAAGAA GGCTGGAGAC CAGGAAGAAG AGAGCTGCGA ATTCTCTAAT 
GCTCTGGAGT ACCTGAAATT ACTGAATTCT TTTGTGGACT CTGTGGGGAT TGTGACCCCG 
CCCTTCTCCA GCAGCTCCGT GCTCAAGTCG GCCCTTGGTC CAGATGTCAT CTGTCGGTGG 
TGGACATCCG CCATCACTGT GGCCATCAGC TGGCTCCAGG GAGACGACGC AGCTGTACGC 
ACTCATTTTA CCGAAGTAGA GCGTGTCCCC AAAGCCCTGG AAGTAACAGA GAGCCCCCTG 
GTGAAGGCCG TCTTCCACAC CTGCAGAGCC ATGCACACCT CACTCTTGGG GAAGGCGGAT 
GGGCAGCAGA GTTCCTTCTG CCACTGTGAG AGGGCCAGCG GCCACCTGTG GAGCAGCCTC 
AACGTTAGCG GGGCCACCTG CGACCTCACC CTCAACCACG TGGTCCAGCT CCTGACCTGT 
GACCTGCTAC TGTCACTGAG GACAGCACTT TGGCAAAAGC AGGCAGGCGC CAGCCAGGCT 
CTGGGCGAGA CGTACCACGC ATCAGGTGCT GAACTGGCTG GCTTCCAGCG GGACCTGGGC 
AGCCTACGCA GGCTGGCTCA CGGCTTCCGC CCAGCATACC GCAAGGTGTT CCTGCATGAA 
GCCACTGTGC GCCTGATGGC AGGAGCCAGC CCCACCCGCA CCCACCAGCT ACTGGAGCAT 
AGTCTGCGCC GGCGGACTGC TCAGAGCACC AAGCACGGAG AGGTGGACAC CTGGCCCGGA 
CAGCGAGAGC GGGCCACCGC CATCCTGCTG GCCTGCCGCC ACCTTCCCCT GTCCTTCCTG 
TCCTCCCCCG GCCAGCGGGC GGTGCTGCTG GCTGAGGCGG CCCGCACCCT GGAGAAGGCG 
GGTGACCGAC GCTCCTGCAA CGACTGCCAG CAGATGATTG TCAAGTTGGG GGGCGGCACG 
GCCATCGCCG CCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC481228 ( XM_022424368.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280076.1 Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X4, mRNA.

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CloneID	OCe291414
Clone ID Related Accession (Same CDS sequence)	XM_022424368.1
Accession Version	XM_022424368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3507bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sterol regulatory element-binding protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAGG TGAAACGACG AGAACAGCTG TTGGCACTGA AAAACCTGGC ACAGCTAAAC 
GATGTTCACC AGCAGTACAA GATTCTTGAT GTCATGCTCA AGGGGCTCTT TAAGGTGCTG 
GAGGACTCAC GGACAGTGCT CATTGCTGCA GATGTGCTCC CAGATGGGCC CTTCCCCCAG 
GACGAGAAAC TGAAGGATGA GATGCTGCAG TTTGTCAGCA ATCAAGTTGG AGAGTTCCCT 
GACTTGTTCT CCGAGCAGCT GTGTAGCTCC TTCCCCAGCG GCGGCGGCGG CGGCGGCGGC 
AATGGCAGCG GCAGTGCTGG GGGCGGAGTG GGGGACCCCG CAATGTCGCG GTCCTTCAGC 
CAGGTCCCGC TACCTGCCTT CTCTCCCGCC GCCGCCTCCC CGCAGGCTCC CGCCCTGCAG 
GTCAAGGCTC CCCCCACCAC GGTCCCTACC CCGCCGCGGG CCACGCCTGT TCTTCAGCCC 
CGCCCCCAGC CCCCGGCCCC GCCCCCGGCC CAGCTGCCGC AGCAGACGGT GATGATCACC 
CCCACGTTCA GCACCGCTCC GCAGACGAGG ATCATCCAGC AGCCTTTGAT ATACCAGAAT 
GCAGCCACCA GCTTTCAAGT CCTTCAGCCT CAAGTCCAAA GCCTAGTGAC ATCCTCCCAG 
GTGCAGCCAG TCACCATCCA GCAGCAGGTG CAGACGGTAC AGGCCCAAAG GGTGCTGACA 
CAGACAGCCA ACGGCACGCT GCAGACCCTC GCCCCGGCCA CGGTACAGAC AGTTGCTGCA 
CCCCAGGTGC AACAGGTCCC GGTCCTGGTA CAGCCTCAGA TCATCAAGAC AGATTCCCTT 
GTTTTGACCA CCTTAAAGAC AGATGGCAGC CCTGTCATGG CCGCAGTCCA GAACCCAGCC 
CTCACCGCCC TCACCACCCC CATCCAGACA GCTGCCCTTC AAGTACCAAC TCTTGTGGGC 
AGCAATGGAA CCATTTTGAC CACAATGCCT GTGATGATGG GGCAAGAGAA AGTGCCCATC 
AAGCAGGTGC CTGGGGGAGT CAAGCAGCTG GAGCCCCCCA AAGAAGGAGA GAGGCGGACG 
ACACACAACA TCATTGAGAA GCGGTATCGT TCCTCTATTA ATGACAAAAT CATTGAGTTG 
AAGGACCTGG TCATGGGGAC AGATGCCAAG ATGCACAAGT CTGGTGTTCT GAGGAAGGCC 
ATTGACTACA TCAAGTACTT GCAGCAAGTC AATCATAAAC TACGCCAGGA GAACATGGTG 
CTGAAGCTGG CAAATCAGAA AAACAAGCTC CTGAAGGGCA TCGACCTAGG CAGCCTGGTG 
GACAATGATG TGGAGCTAAA GATTGAAGAC TTCAATCAGA ATATCCTTCT GATGTCCCCT 
CCGGCCTCTG ACTCAGGGTC CCAGGCTGGT TTCTCCCCCT ACTCCATCGA CTCCGAGCCA 
GGAAGCCCTC TGTTGGATGA TGCAAAGGTC AAAGATGAGC CAGACTCTCC TCCCGTGGCG 
CTGGGCATGG TGGACCGCTC TCGGATTCTG CTCTGTGTGC TCACCTTCCT CTGCCTCTCC 
TTTAACCCCC TGACTGCCCT GTTGCAGTGG GGAGGGGCCC ACAGCTCTGA CCAGCACCCA 
TATTCCGGCT CTGGCCGCAG CATCCTGTCA CTTGAGTCAG GTTCTGGGGG CTGGTTTGAT 
TGGATGATGC CAACGCTTCT CTTATGGCTG ATAAATGGTG TGATTGTCCT GAGCGTCTTT 
GTGAAGCTGC TGGTCCATGG AGAGCCAGTG ATCCGGCCAC ATTCACGCTC CTCAGTCACC 
TTCTGGAGAC ACCGGAAGCA GGCCGACCTG GACCTCGCCA GGGGGGATTT TGCAGCTGCT 
GCAGCCAACC TGCAAACCTG CCTGTCGGTG TTGGGCCGCA CACTGCCCAC CTCCCGCCTG 
GACCTGGCCT GCAGCCTCTC CTGGAACGTG ATCCGCTACA GCCTGCAGAA GCTGCGCCTG 
GTGCGCTGGC TGCTCAAGAA GGTCTTCCAG CACCGGCGGG CTGCCCCGGC CACCACCGCA 
GGCTTTGAGG ACGAGGCGAA GGCCAGCGCC CGGGACGCGG CCCTGGCCTA TCACAGGCTG 
CACCAGCTGC ACATCACTGG GAAGCTTCCT GCGGGATCCG CCTGTTCAGA TGTACACATG 
GCTTTGTGCG CCGTGAACCT GGCGGAATGT GCAGAGGAGA AAATCCCACC GAGCACGTTG 
GTTGAGATCC ATCTGACTGC CGCCATGGGG CTCAAGACCC AGTGTGGAGG CAAGCTTGGC 
TTCCTGGCGA GCTACTTCCT CAGTAGAGCC CAGAGCCTGT GTGGCCCGGA GCGCAGCGCC 
GTCCCTGACT CACTGCGCTG GCTGTGCCAC CCCCTGGGCC AGAAGTTTTT CATGGAGCGG 
AGCTGGTCGG TGAAGTCAGC TGCCAAGGAG AGTCTCTACT GTGCTCAGAG GAACCCAGCT 
GACCCCATCG CACAGGTCCA CCAGGCCTTC TGCAAGAACC TACTGGAGCG AGCAGTTGAA 
TCACTGGTGA AGCCTCAGGC CAAGAAGAAG GCTGGAGACC AGGAAGAAGA GAGCTGCGAA 
TTCTCTAATG CTCTGGAGTA CCTGAAATTA CTGAATTCTT TTGTGGACTC TGTGGGGATT 
GTGACCCCGC CCTTCTCCAG CAGCTCCGTG CTCAAGTCGG CCCTTGGTCC AGATGTCATC 
TGTCGGTGGT GGACATCCGC CATCACTGTG GCCATCAGCT GGCTCCAGGG AGACGACGCA 
GCTGTACGCA CTCATTTTAC CGAAGTAGAG CGTGTCCCCA AAGCCCTGGA AGTAACAGAG 
AGCCCCCTGG TGAAGGCCGT CTTCCACACC TGCAGAGCCA TGCACACCTC ACTCTTGGGG 
AAGGCGGATG GGCAGCAGAG TTCCTTCTGC CACTGTGAGA GGGCCAGCGG CCACCTGTGG 
AGCAGCCTCA ACGTTAGCGG GGCCACCTGC GACCTCACCC TCAACCACGT GGTCCAGCTC 
CTGACCTGTG ACCTGCTACT GTCACTGAGG ACAGCACTTT GGCAAAAGCA GGCAGGCGCC 
AGCCAGGCTC TGGGCGAGAC GTACCACGCA TCAGGTGCTG AACTGGCTGG CTTCCAGCGG 
GACCTGGGCA GCCTACGCAG GCTGGCTCAC GGCTTCCGCC CAGCATACCG CAAGGTGTTC 
CTGCATGAAG CCACTGTGCG CCTGATGGCA GGAGCCAGCC CCACCCGCAC CCACCAGCTA 
CTGGAGCATA GTCTGCGCCG GCGGACTGCT CAGAGCACCA AGCACGGAGA GGTGGACACC 
TGGCCCGGAC AGCGAGAGCG GGCCACCGCC ATCCTGCTGG CCTGCCGCCA CCTTCCCCTG 
TCCTTCCTGT CCTCCCCCGG CCAGCGGGCG GTGCTGCTGG CTGAGGCGGC CCGCACCCTG 
GAGAAGGCGG GTGACCGACG CTCCTGCAAC GACTGCCAGC AGATGATTGT CAAGTTGGGG 
GGCGGCACGG CCATCGCCGC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280076.1
CDS	184..3690
Translation	MFEVKRREQLLALKNLAQLNDVHQQYKILDVMLKGLFKVLEDSRTVLIAADVLPDGPFPQDEKLKDEMLQFVSNQVGEFPDLFSEQLCSSFPSGGGGGGGNGSGSAGGGVGDPAMSRSFSQVPLPAFSPAAASPQAPALQVKAPPTTVPTPPRATPVLQPRPQPPAPPPAQLPQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNDVELKIEDFNQNILLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTALLQWGGAHSSDQHPYSGSGRSILSLESGSGGWFDWMMPTLLLWLINGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAANLQTCLSVLGRTLPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQHRRAAPATTAGFEDEAKASARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTQCGGKLGFLASYFLSRAQSLCGPERSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSNALEYLKLLNSFVDSVGIVTPPFSSSSVLKSALGPDVICRWWTSAITVAISWLQGDDAAVRTHFTEVERVPKALEVTESPLVKAVFHTCRAMHTSLLGKADGQQSSFCHCERASGHLWSSLNVSGATCDLTLNHVVQLLTCDLLLSLRTALWQKQAGASQALGETYHASGAELAGFQRDLGSLRRLAHGFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTAQSTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKAGDRRSCNDCQQMIVKLGGGTAIAAS

Target ORF information:

RefSeq Version	XM_022424368.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424368.1

ORF Insert Sequence:

ATGTTTGAGG TGAAACGACG AGAACAGCTG TTGGCACTGA AAAACCTGGC ACAGCTAAAC 
GATGTTCACC AGCAGTACAA GATTCTTGAT GTCATGCTCA AGGGGCTCTT TAAGGTGCTG 
GAGGACTCAC GGACAGTGCT CATTGCTGCA GATGTGCTCC CAGATGGGCC CTTCCCCCAG 
GACGAGAAAC TGAAGGATGA GATGCTGCAG TTTGTCAGCA ATCAAGTTGG AGAGTTCCCT 
GACTTGTTCT CCGAGCAGCT GTGTAGCTCC TTCCCCAGCG GCGGCGGCGG CGGCGGCGGC 
AATGGCAGCG GCAGTGCTGG GGGCGGAGTG GGGGACCCCG CAATGTCGCG GTCCTTCAGC 
CAGGTCCCGC TACCTGCCTT CTCTCCCGCC GCCGCCTCCC CGCAGGCTCC CGCCCTGCAG 
GTCAAGGCTC CCCCCACCAC GGTCCCTACC CCGCCGCGGG CCACGCCTGT TCTTCAGCCC 
CGCCCCCAGC CCCCGGCCCC GCCCCCGGCC CAGCTGCCGC AGCAGACGGT GATGATCACC 
CCCACGTTCA GCACCGCTCC GCAGACGAGG ATCATCCAGC AGCCTTTGAT ATACCAGAAT 
GCAGCCACCA GCTTTCAAGT CCTTCAGCCT CAAGTCCAAA GCCTAGTGAC ATCCTCCCAG 
GTGCAGCCAG TCACCATCCA GCAGCAGGTG CAGACGGTAC AGGCCCAAAG GGTGCTGACA 
CAGACAGCCA ACGGCACGCT GCAGACCCTC GCCCCGGCCA CGGTACAGAC AGTTGCTGCA 
CCCCAGGTGC AACAGGTCCC GGTCCTGGTA CAGCCTCAGA TCATCAAGAC AGATTCCCTT 
GTTTTGACCA CCTTAAAGAC AGATGGCAGC CCTGTCATGG CCGCAGTCCA GAACCCAGCC 
CTCACCGCCC TCACCACCCC CATCCAGACA GCTGCCCTTC AAGTACCAAC TCTTGTGGGC 
AGCAATGGAA CCATTTTGAC CACAATGCCT GTGATGATGG GGCAAGAGAA AGTGCCCATC 
AAGCAGGTGC CTGGGGGAGT CAAGCAGCTG GAGCCCCCCA AAGAAGGAGA GAGGCGGACG 
ACACACAACA TCATTGAGAA GCGGTATCGT TCCTCTATTA ATGACAAAAT CATTGAGTTG 
AAGGACCTGG TCATGGGGAC AGATGCCAAG ATGCACAAGT CTGGTGTTCT GAGGAAGGCC 
ATTGACTACA TCAAGTACTT GCAGCAAGTC AATCATAAAC TACGCCAGGA GAACATGGTG 
CTGAAGCTGG CAAATCAGAA AAACAAGCTC CTGAAGGGCA TCGACCTAGG CAGCCTGGTG 
GACAATGATG TGGAGCTAAA GATTGAAGAC TTCAATCAGA ATATCCTTCT GATGTCCCCT 
CCGGCCTCTG ACTCAGGGTC CCAGGCTGGT TTCTCCCCCT ACTCCATCGA CTCCGAGCCA 
GGAAGCCCTC TGTTGGATGA TGCAAAGGTC AAAGATGAGC CAGACTCTCC TCCCGTGGCG 
CTGGGCATGG TGGACCGCTC TCGGATTCTG CTCTGTGTGC TCACCTTCCT CTGCCTCTCC 
TTTAACCCCC TGACTGCCCT GTTGCAGTGG GGAGGGGCCC ACAGCTCTGA CCAGCACCCA 
TATTCCGGCT CTGGCCGCAG CATCCTGTCA CTTGAGTCAG GTTCTGGGGG CTGGTTTGAT 
TGGATGATGC CAACGCTTCT CTTATGGCTG ATAAATGGTG TGATTGTCCT GAGCGTCTTT 
GTGAAGCTGC TGGTCCATGG AGAGCCAGTG ATCCGGCCAC ATTCACGCTC CTCAGTCACC 
TTCTGGAGAC ACCGGAAGCA GGCCGACCTG GACCTCGCCA GGGGGGATTT TGCAGCTGCT 
GCAGCCAACC TGCAAACCTG CCTGTCGGTG TTGGGCCGCA CACTGCCCAC CTCCCGCCTG 
GACCTGGCCT GCAGCCTCTC CTGGAACGTG ATCCGCTACA GCCTGCAGAA GCTGCGCCTG 
GTGCGCTGGC TGCTCAAGAA GGTCTTCCAG CACCGGCGGG CTGCCCCGGC CACCACCGCA 
GGCTTTGAGG ACGAGGCGAA GGCCAGCGCC CGGGACGCGG CCCTGGCCTA TCACAGGCTG 
CACCAGCTGC ACATCACTGG GAAGCTTCCT GCGGGATCCG CCTGTTCAGA TGTACACATG 
GCTTTGTGCG CCGTGAACCT GGCGGAATGT GCAGAGGAGA AAATCCCACC GAGCACGTTG 
GTTGAGATCC ATCTGACTGC CGCCATGGGG CTCAAGACCC AGTGTGGAGG CAAGCTTGGC 
TTCCTGGCGA GCTACTTCCT CAGTAGAGCC CAGAGCCTGT GTGGCCCGGA GCGCAGCGCC 
GTCCCTGACT CACTGCGCTG GCTGTGCCAC CCCCTGGGCC AGAAGTTTTT CATGGAGCGG 
AGCTGGTCGG TGAAGTCAGC TGCCAAGGAG AGTCTCTACT GTGCTCAGAG GAACCCAGCT 
GACCCCATCG CACAGGTCCA CCAGGCCTTC TGCAAGAACC TACTGGAGCG AGCAGTTGAA 
TCACTGGTGA AGCCTCAGGC CAAGAAGAAG GCTGGAGACC AGGAAGAAGA GAGCTGCGAA 
TTCTCTAATG CTCTGGAGTA CCTGAAATTA CTGAATTCTT TTGTGGACTC TGTGGGGATT 
GTGACCCCGC CCTTCTCCAG CAGCTCCGTG CTCAAGTCGG CCCTTGGTCC AGATGTCATC 
TGTCGGTGGT GGACATCCGC CATCACTGTG GCCATCAGCT GGCTCCAGGG AGACGACGCA 
GCTGTACGCA CTCATTTTAC CGAAGTAGAG CGTGTCCCCA AAGCCCTGGA AGTAACAGAG 
AGCCCCCTGG TGAAGGCCGT CTTCCACACC TGCAGAGCCA TGCACACCTC ACTCTTGGGG 
AAGGCGGATG GGCAGCAGAG TTCCTTCTGC CACTGTGAGA GGGCCAGCGG CCACCTGTGG 
AGCAGCCTCA ACGTTAGCGG GGCCACCTGC GACCTCACCC TCAACCACGT GGTCCAGCTC 
CTGACCTGTG ACCTGCTACT GTCACTGAGG ACAGCACTTT GGCAAAAGCA GGCAGGCGCC 
AGCCAGGCTC TGGGCGAGAC GTACCACGCA TCAGGTGCTG AACTGGCTGG CTTCCAGCGG 
GACCTGGGCA GCCTACGCAG GCTGGCTCAC GGCTTCCGCC CAGCATACCG CAAGGTGTTC 
CTGCATGAAG CCACTGTGCG CCTGATGGCA GGAGCCAGCC CCACCCGCAC CCACCAGCTA 
CTGGAGCATA GTCTGCGCCG GCGGACTGCT CAGAGCACCA AGCACGGAGA GGTGGACACC 
TGGCCCGGAC AGCGAGAGCG GGCCACCGCC ATCCTGCTGG CCTGCCGCCA CCTTCCCCTG 
TCCTTCCTGT CCTCCCCCGG CCAGCGGGCG GTGCTGCTGG CTGAGGCGGC CCGCACCCTG 
GAGAAGGCGG GTGACCGACG CTCCTGCAAC GACTGCCAGC AGATGATTGT CAAGTTGGGG 
GGCGGCACGG CCATCGCCGC CTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC481228 ( XM_014117321.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013972796.1 Canis lupus familiaris sterol regulatory element binding transcription factor 2 (SREBF2), transcript variant X2, mRNA.

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CloneID	OCe38886
Clone ID Related Accession (Same CDS sequence)	XM_014117321.1 , XM_022424367.1
Accession Version	XM_014117321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	sterol regulatory element-binding protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726078.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG 
TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG 
GGCGGAGTGG GGGACCCCGC AATGTCGCGG TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC 
TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC GCCCTGCAGG TCAAGGCTCC CCCCACCACG 
GTCCCTACCC CGCCGCGGGC CACGCCTGTT CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG 
CCCCCGGCCC AGCTGCCGCA GCAGACGGTG ATGATCACCC CCACGTTCAG CACCGCTCCG 
CAGACGAGGA TCATCCAGCA GCCTTTGATA TACCAGAATG CAGCCACCAG CTTTCAAGTC 
CTTCAGCCTC AAGTCCAAAG CCTAGTGACA TCCTCCCAGG TGCAGCCAGT CACCATCCAG 
CAGCAGGTGC AGACGGTACA GGCCCAAAGG GTGCTGACAC AGACAGCCAA CGGCACGCTG 
CAGACCCTCG CCCCGGCCAC GGTACAGACA GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG 
GTCCTGGTAC AGCCTCAGAT CATCAAGACA GATTCCCTTG TTTTGACCAC CTTAAAGACA 
GATGGCAGCC CTGTCATGGC CGCAGTCCAG AACCCAGCCC TCACCGCCCT CACCACCCCC 
ATCCAGACAG CTGCCCTTCA AGTACCAACT CTTGTGGGCA GCAATGGAAC CATTTTGACC 
ACAATGCCTG TGATGATGGG GCAAGAGAAA GTGCCCATCA AGCAGGTGCC TGGGGGAGTC 
AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG AGGCGGACGA CACACAACAT CATTGAGAAG 
CGGTATCGTT CCTCTATTAA TGACAAAATC ATTGAGTTGA AGGACCTGGT CATGGGGACA 
GATGCCAAGA TGCACAAGTC TGGTGTTCTG AGGAAGGCCA TTGACTACAT CAAGTACTTG 
CAGCAAGTCA ATCATAAACT ACGCCAGGAG AACATGGTGC TGAAGCTGGC AAATCAGAAA 
AACAAGCTCC TGAAGGGCAT CGACCTAGGC AGCCTGGTGG ACAATGATGT GGAGCTAAAG 
ATTGAAGACT TCAATCAGAA TATCCTTCTG ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC 
CAGGCTGGTT TCTCCCCCTA CTCCATCGAC TCCGAGCCAG GAAGCCCTCT GTTGGATGAT 
GCAAAGGTCA AAGATGAGCC AGACTCTCCT CCCGTGGCGC TGGGCATGGT GGACCGCTCT 
CGGATTCTGC TCTGTGTGCT CACCTTCCTC TGCCTCTCCT TTAACCCCCT GACTGCCCTG 
TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC CAGCACCCAT ATTCCGGCTC TGGCCGCAGC 
ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC TGGTTTGATT GGATGATGCC AACGCTTCTC 
TTATGGCTGA TAAATGGTGT GATTGTCCTG AGCGTCTTTG TGAAGCTGCT GGTCCATGGA 
GAGCCAGTGA TCCGGCCACA TTCACGCTCC TCAGTCACCT TCTGGAGACA CCGGAAGCAG 
GCCGACCTGG ACCTCGCCAG GGGGGATTTT GCAGCTGCTG CAGCCAACCT GCAAACCTGC 
CTGTCGGTGT TGGGCCGCAC ACTGCCCACC TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC 
TGGAACGTGA TCCGCTACAG CCTGCAGAAG CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG 
GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC ACCACCGCAG GCTTTGAGGA CGAGGCGAAG 
GCCAGCGCCC GGGACGCGGC CCTGGCCTAT CACAGGCTGC ACCAGCTGCA CATCACTGGG 
AAGCTTCCTG CGGGATCCGC CTGTTCAGAT GTACACATGG CTTTGTGCGC CGTGAACCTG 
GCGGAATGTG CAGAGGAGAA AATCCCACCG AGCACGTTGG TTGAGATCCA TCTGACTGCC 
GCCATGGGGC TCAAGACCCA GTGTGGAGGC AAGCTTGGCT TCCTGGCGAG CTACTTCCTC 
AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG CGCAGCGCCG TCCCTGACTC ACTGCGCTGG 
CTGTGCCACC CCCTGGGCCA GAAGTTTTTC ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT 
GCCAAGGAGA GTCTCTACTG TGCTCAGAGG AACCCAGCTG ACCCCATCGC ACAGGTCCAC 
CAGGCCTTCT GCAAGAACCT ACTGGAGCGA GCAGTTGAAT CACTGGTGAA GCCTCAGGCC 
AAGAAGAAGG CTGGAGACCA GGAAGAAGAG AGCTGCGAAT TCTCTAATGC TCTGGAGTAC 
CTGAAATTAC TGAATTCTTT TGTGGACTCT GTGGGGATTG TGACCCCGCC CTTCTCCAGC 
AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA GATGTCATCT GTCGGTGGTG GACATCCGCC 
ATCACTGTGG CCATCAGCTG GCTCCAGGGA GACGACGCAG CTGTACGCAC TCATTTTACC 
GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA GTAACAGAGA GCCCCCTGGT GAAGGCCGTC 
TTCCACACCT GCAGAGCCAT GCACACCTCA CTCTTGGGGA AGGCGGATGG GCAGCAGAGT 
TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC CACCTGTGGA GCAGCCTCAA CGTTAGCGGG 
GCCACCTGCG ACCTCACCCT CAACCACGTG GTCCAGCTCC TGACCTGTGA CCTGCTACTG 
TCACTGAGGA CAGCACTTTG GCAAAAGCAG GCAGGCGCCA GCCAGGCTCT GGGCGAGACG 
TACCACGCAT CAGGTGCTGA ACTGGCTGGC TTCCAGCGGG ACCTGGGCAG CCTACGCAGG 
CTGGCTCACG GCTTCCGCCC AGCATACCGC AAGGTGTTCC TGCATGAAGC CACTGTGCGC 
CTGATGGCAG GAGCCAGCCC CACCCGCACC CACCAGCTAC TGGAGCATAG TCTGCGCCGG 
CGGACTGCTC AGAGCACCAA GCACGGAGAG GTGGACACCT GGCCCGGACA GCGAGAGCGG 
GCCACCGCCA TCCTGCTGGC CTGCCGCCAC CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC 
CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC CGCACCCTGG AGAAGGCGGG TGACCGACGC 
TCCTGCAACG ACTGCCAGCA GATGATTGTC AAGTTGGGGG GCGGCACGGC CATCGCCGCC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013972796.1
CDS	330..3635
Translation	MLQFVSNQVGEFPDLFSEQLCSSFPSGGGGGGGNGSGSAGGGVGDPAMSRSFSQVPLPAFSPAAASPQAPALQVKAPPTTVPTPPRATPVLQPRPQPPAPPPAQLPQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNDVELKIEDFNQNILLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTALLQWGGAHSSDQHPYSGSGRSILSLESGSGGWFDWMMPTLLLWLINGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAANLQTCLSVLGRTLPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQHRRAAPATTAGFEDEAKASARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTQCGGKLGFLASYFLSRAQSLCGPERSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSNALEYLKLLNSFVDSVGIVTPPFSSSSVLKSALGPDVICRWWTSAITVAISWLQGDDAAVRTHFTEVERVPKALEVTESPLVKAVFHTCRAMHTSLLGKADGQQSSFCHCERASGHLWSSLNVSGATCDLTLNHVVQLLTCDLLLSLRTALWQKQAGASQALGETYHASGAELAGFQRDLGSLRRLAHGFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTAQSTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKAGDRRSCNDCQQMIVKLGGGTAIAAS

Target ORF information:

RefSeq Version	XM_014117321.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris sterol regulatory element binding transcription factor 2 (SREBF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014117321.1

ORF Insert Sequence:

ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG 
TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG 
GGCGGAGTGG GGGACCCCGC AATGTCGCGG TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC 
TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC GCCCTGCAGG TCAAGGCTCC CCCCACCACG 
GTCCCTACCC CGCCGCGGGC CACGCCTGTT CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG 
CCCCCGGCCC AGCTGCCGCA GCAGACGGTG ATGATCACCC CCACGTTCAG CACCGCTCCG 
CAGACGAGGA TCATCCAGCA GCCTTTGATA TACCAGAATG CAGCCACCAG CTTTCAAGTC 
CTTCAGCCTC AAGTCCAAAG CCTAGTGACA TCCTCCCAGG TGCAGCCAGT CACCATCCAG 
CAGCAGGTGC AGACGGTACA GGCCCAAAGG GTGCTGACAC AGACAGCCAA CGGCACGCTG 
CAGACCCTCG CCCCGGCCAC GGTACAGACA GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG 
GTCCTGGTAC AGCCTCAGAT CATCAAGACA GATTCCCTTG TTTTGACCAC CTTAAAGACA 
GATGGCAGCC CTGTCATGGC CGCAGTCCAG AACCCAGCCC TCACCGCCCT CACCACCCCC 
ATCCAGACAG CTGCCCTTCA AGTACCAACT CTTGTGGGCA GCAATGGAAC CATTTTGACC 
ACAATGCCTG TGATGATGGG GCAAGAGAAA GTGCCCATCA AGCAGGTGCC TGGGGGAGTC 
AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG AGGCGGACGA CACACAACAT CATTGAGAAG 
CGGTATCGTT CCTCTATTAA TGACAAAATC ATTGAGTTGA AGGACCTGGT CATGGGGACA 
GATGCCAAGA TGCACAAGTC TGGTGTTCTG AGGAAGGCCA TTGACTACAT CAAGTACTTG 
CAGCAAGTCA ATCATAAACT ACGCCAGGAG AACATGGTGC TGAAGCTGGC AAATCAGAAA 
AACAAGCTCC TGAAGGGCAT CGACCTAGGC AGCCTGGTGG ACAATGATGT GGAGCTAAAG 
ATTGAAGACT TCAATCAGAA TATCCTTCTG ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC 
CAGGCTGGTT TCTCCCCCTA CTCCATCGAC TCCGAGCCAG GAAGCCCTCT GTTGGATGAT 
GCAAAGGTCA AAGATGAGCC AGACTCTCCT CCCGTGGCGC TGGGCATGGT GGACCGCTCT 
CGGATTCTGC TCTGTGTGCT CACCTTCCTC TGCCTCTCCT TTAACCCCCT GACTGCCCTG 
TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC CAGCACCCAT ATTCCGGCTC TGGCCGCAGC 
ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC TGGTTTGATT GGATGATGCC AACGCTTCTC 
TTATGGCTGA TAAATGGTGT GATTGTCCTG AGCGTCTTTG TGAAGCTGCT GGTCCATGGA 
GAGCCAGTGA TCCGGCCACA TTCACGCTCC TCAGTCACCT TCTGGAGACA CCGGAAGCAG 
GCCGACCTGG ACCTCGCCAG GGGGGATTTT GCAGCTGCTG CAGCCAACCT GCAAACCTGC 
CTGTCGGTGT TGGGCCGCAC ACTGCCCACC TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC 
TGGAACGTGA TCCGCTACAG CCTGCAGAAG CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG 
GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC ACCACCGCAG GCTTTGAGGA CGAGGCGAAG 
GCCAGCGCCC GGGACGCGGC CCTGGCCTAT CACAGGCTGC ACCAGCTGCA CATCACTGGG 
AAGCTTCCTG CGGGATCCGC CTGTTCAGAT GTACACATGG CTTTGTGCGC CGTGAACCTG 
GCGGAATGTG CAGAGGAGAA AATCCCACCG AGCACGTTGG TTGAGATCCA TCTGACTGCC 
GCCATGGGGC TCAAGACCCA GTGTGGAGGC AAGCTTGGCT TCCTGGCGAG CTACTTCCTC 
AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG CGCAGCGCCG TCCCTGACTC ACTGCGCTGG 
CTGTGCCACC CCCTGGGCCA GAAGTTTTTC ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT 
GCCAAGGAGA GTCTCTACTG TGCTCAGAGG AACCCAGCTG ACCCCATCGC ACAGGTCCAC 
CAGGCCTTCT GCAAGAACCT ACTGGAGCGA GCAGTTGAAT CACTGGTGAA GCCTCAGGCC 
AAGAAGAAGG CTGGAGACCA GGAAGAAGAG AGCTGCGAAT TCTCTAATGC TCTGGAGTAC 
CTGAAATTAC TGAATTCTTT TGTGGACTCT GTGGGGATTG TGACCCCGCC CTTCTCCAGC 
AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA GATGTCATCT GTCGGTGGTG GACATCCGCC 
ATCACTGTGG CCATCAGCTG GCTCCAGGGA GACGACGCAG CTGTACGCAC TCATTTTACC 
GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA GTAACAGAGA GCCCCCTGGT GAAGGCCGTC 
TTCCACACCT GCAGAGCCAT GCACACCTCA CTCTTGGGGA AGGCGGATGG GCAGCAGAGT 
TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC CACCTGTGGA GCAGCCTCAA CGTTAGCGGG 
GCCACCTGCG ACCTCACCCT CAACCACGTG GTCCAGCTCC TGACCTGTGA CCTGCTACTG 
TCACTGAGGA CAGCACTTTG GCAAAAGCAG GCAGGCGCCA GCCAGGCTCT GGGCGAGACG 
TACCACGCAT CAGGTGCTGA ACTGGCTGGC TTCCAGCGGG ACCTGGGCAG CCTACGCAGG 
CTGGCTCACG GCTTCCGCCC AGCATACCGC AAGGTGTTCC TGCATGAAGC CACTGTGCGC 
CTGATGGCAG GAGCCAGCCC CACCCGCACC CACCAGCTAC TGGAGCATAG TCTGCGCCGG 
CGGACTGCTC AGAGCACCAA GCACGGAGAG GTGGACACCT GGCCCGGACA GCGAGAGCGG 
GCCACCGCCA TCCTGCTGGC CTGCCGCCAC CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC 
CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC CGCACCCTGG AGAAGGCGGG TGACCGACGC 
TCCTGCAACG ACTGCCAGCA GATGATTGTC AAGTTGGGGG GCGGCACGGC CATCGCCGCC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC481228 ( XM_022424367.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022280075.1 Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X3, mRNA.

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CloneID	OCe38886
Clone ID Related Accession (Same CDS sequence)	XM_014117321.1 , XM_022424367.1
Accession Version	XM_022424367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	sterol regulatory element-binding protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006592.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG 
TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG 
GGCGGAGTGG GGGACCCCGC AATGTCGCGG TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC 
TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC GCCCTGCAGG TCAAGGCTCC CCCCACCACG 
GTCCCTACCC CGCCGCGGGC CACGCCTGTT CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG 
CCCCCGGCCC AGCTGCCGCA GCAGACGGTG ATGATCACCC CCACGTTCAG CACCGCTCCG 
CAGACGAGGA TCATCCAGCA GCCTTTGATA TACCAGAATG CAGCCACCAG CTTTCAAGTC 
CTTCAGCCTC AAGTCCAAAG CCTAGTGACA TCCTCCCAGG TGCAGCCAGT CACCATCCAG 
CAGCAGGTGC AGACGGTACA GGCCCAAAGG GTGCTGACAC AGACAGCCAA CGGCACGCTG 
CAGACCCTCG CCCCGGCCAC GGTACAGACA GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG 
GTCCTGGTAC AGCCTCAGAT CATCAAGACA GATTCCCTTG TTTTGACCAC CTTAAAGACA 
GATGGCAGCC CTGTCATGGC CGCAGTCCAG AACCCAGCCC TCACCGCCCT CACCACCCCC 
ATCCAGACAG CTGCCCTTCA AGTACCAACT CTTGTGGGCA GCAATGGAAC CATTTTGACC 
ACAATGCCTG TGATGATGGG GCAAGAGAAA GTGCCCATCA AGCAGGTGCC TGGGGGAGTC 
AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG AGGCGGACGA CACACAACAT CATTGAGAAG 
CGGTATCGTT CCTCTATTAA TGACAAAATC ATTGAGTTGA AGGACCTGGT CATGGGGACA 
GATGCCAAGA TGCACAAGTC TGGTGTTCTG AGGAAGGCCA TTGACTACAT CAAGTACTTG 
CAGCAAGTCA ATCATAAACT ACGCCAGGAG AACATGGTGC TGAAGCTGGC AAATCAGAAA 
AACAAGCTCC TGAAGGGCAT CGACCTAGGC AGCCTGGTGG ACAATGATGT GGAGCTAAAG 
ATTGAAGACT TCAATCAGAA TATCCTTCTG ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC 
CAGGCTGGTT TCTCCCCCTA CTCCATCGAC TCCGAGCCAG GAAGCCCTCT GTTGGATGAT 
GCAAAGGTCA AAGATGAGCC AGACTCTCCT CCCGTGGCGC TGGGCATGGT GGACCGCTCT 
CGGATTCTGC TCTGTGTGCT CACCTTCCTC TGCCTCTCCT TTAACCCCCT GACTGCCCTG 
TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC CAGCACCCAT ATTCCGGCTC TGGCCGCAGC 
ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC TGGTTTGATT GGATGATGCC AACGCTTCTC 
TTATGGCTGA TAAATGGTGT GATTGTCCTG AGCGTCTTTG TGAAGCTGCT GGTCCATGGA 
GAGCCAGTGA TCCGGCCACA TTCACGCTCC TCAGTCACCT TCTGGAGACA CCGGAAGCAG 
GCCGACCTGG ACCTCGCCAG GGGGGATTTT GCAGCTGCTG CAGCCAACCT GCAAACCTGC 
CTGTCGGTGT TGGGCCGCAC ACTGCCCACC TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC 
TGGAACGTGA TCCGCTACAG CCTGCAGAAG CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG 
GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC ACCACCGCAG GCTTTGAGGA CGAGGCGAAG 
GCCAGCGCCC GGGACGCGGC CCTGGCCTAT CACAGGCTGC ACCAGCTGCA CATCACTGGG 
AAGCTTCCTG CGGGATCCGC CTGTTCAGAT GTACACATGG CTTTGTGCGC CGTGAACCTG 
GCGGAATGTG CAGAGGAGAA AATCCCACCG AGCACGTTGG TTGAGATCCA TCTGACTGCC 
GCCATGGGGC TCAAGACCCA GTGTGGAGGC AAGCTTGGCT TCCTGGCGAG CTACTTCCTC 
AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG CGCAGCGCCG TCCCTGACTC ACTGCGCTGG 
CTGTGCCACC CCCTGGGCCA GAAGTTTTTC ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT 
GCCAAGGAGA GTCTCTACTG TGCTCAGAGG AACCCAGCTG ACCCCATCGC ACAGGTCCAC 
CAGGCCTTCT GCAAGAACCT ACTGGAGCGA GCAGTTGAAT CACTGGTGAA GCCTCAGGCC 
AAGAAGAAGG CTGGAGACCA GGAAGAAGAG AGCTGCGAAT TCTCTAATGC TCTGGAGTAC 
CTGAAATTAC TGAATTCTTT TGTGGACTCT GTGGGGATTG TGACCCCGCC CTTCTCCAGC 
AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA GATGTCATCT GTCGGTGGTG GACATCCGCC 
ATCACTGTGG CCATCAGCTG GCTCCAGGGA GACGACGCAG CTGTACGCAC TCATTTTACC 
GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA GTAACAGAGA GCCCCCTGGT GAAGGCCGTC 
TTCCACACCT GCAGAGCCAT GCACACCTCA CTCTTGGGGA AGGCGGATGG GCAGCAGAGT 
TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC CACCTGTGGA GCAGCCTCAA CGTTAGCGGG 
GCCACCTGCG ACCTCACCCT CAACCACGTG GTCCAGCTCC TGACCTGTGA CCTGCTACTG 
TCACTGAGGA CAGCACTTTG GCAAAAGCAG GCAGGCGCCA GCCAGGCTCT GGGCGAGACG 
TACCACGCAT CAGGTGCTGA ACTGGCTGGC TTCCAGCGGG ACCTGGGCAG CCTACGCAGG 
CTGGCTCACG GCTTCCGCCC AGCATACCGC AAGGTGTTCC TGCATGAAGC CACTGTGCGC 
CTGATGGCAG GAGCCAGCCC CACCCGCACC CACCAGCTAC TGGAGCATAG TCTGCGCCGG 
CGGACTGCTC AGAGCACCAA GCACGGAGAG GTGGACACCT GGCCCGGACA GCGAGAGCGG 
GCCACCGCCA TCCTGCTGGC CTGCCGCCAC CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC 
CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC CGCACCCTGG AGAAGGCGGG TGACCGACGC 
TCCTGCAACG ACTGCCAGCA GATGATTGTC AAGTTGGGGG GCGGCACGGC CATCGCCGCC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022280075.1
CDS	58..3363
Translation	MLQFVSNQVGEFPDLFSEQLCSSFPSGGGGGGGNGSGSAGGGVGDPAMSRSFSQVPLPAFSPAAASPQAPALQVKAPPTTVPTPPRATPVLQPRPQPPAPPPAQLPQQTVMITPTFSTAPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSNGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNDVELKIEDFNQNILLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTALLQWGGAHSSDQHPYSGSGRSILSLESGSGGWFDWMMPTLLLWLINGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAANLQTCLSVLGRTLPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQHRRAAPATTAGFEDEAKASARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTQCGGKLGFLASYFLSRAQSLCGPERSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAVESLVKPQAKKKAGDQEEESCEFSNALEYLKLLNSFVDSVGIVTPPFSSSSVLKSALGPDVICRWWTSAITVAISWLQGDDAAVRTHFTEVERVPKALEVTESPLVKAVFHTCRAMHTSLLGKADGQQSSFCHCERASGHLWSSLNVSGATCDLTLNHVVQLLTCDLLLSLRTALWQKQAGASQALGETYHASGAELAGFQRDLGSLRRLAHGFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTAQSTKHGEVDTWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKAGDRRSCNDCQQMIVKLGGGTAIAAS

Target ORF information:

RefSeq Version	XM_022424367.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coiled-coil domain-containing protein 134 (LOC481228), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022424367.1

ORF Insert Sequence:

ATGCTGCAGT TTGTCAGCAA TCAAGTTGGA GAGTTCCCTG ACTTGTTCTC CGAGCAGCTG 
TGTAGCTCCT TCCCCAGCGG CGGCGGCGGC GGCGGCGGCA ATGGCAGCGG CAGTGCTGGG 
GGCGGAGTGG GGGACCCCGC AATGTCGCGG TCCTTCAGCC AGGTCCCGCT ACCTGCCTTC 
TCTCCCGCCG CCGCCTCCCC GCAGGCTCCC GCCCTGCAGG TCAAGGCTCC CCCCACCACG 
GTCCCTACCC CGCCGCGGGC CACGCCTGTT CTTCAGCCCC GCCCCCAGCC CCCGGCCCCG 
CCCCCGGCCC AGCTGCCGCA GCAGACGGTG ATGATCACCC CCACGTTCAG CACCGCTCCG 
CAGACGAGGA TCATCCAGCA GCCTTTGATA TACCAGAATG CAGCCACCAG CTTTCAAGTC 
CTTCAGCCTC AAGTCCAAAG CCTAGTGACA TCCTCCCAGG TGCAGCCAGT CACCATCCAG 
CAGCAGGTGC AGACGGTACA GGCCCAAAGG GTGCTGACAC AGACAGCCAA CGGCACGCTG 
CAGACCCTCG CCCCGGCCAC GGTACAGACA GTTGCTGCAC CCCAGGTGCA ACAGGTCCCG 
GTCCTGGTAC AGCCTCAGAT CATCAAGACA GATTCCCTTG TTTTGACCAC CTTAAAGACA 
GATGGCAGCC CTGTCATGGC CGCAGTCCAG AACCCAGCCC TCACCGCCCT CACCACCCCC 
ATCCAGACAG CTGCCCTTCA AGTACCAACT CTTGTGGGCA GCAATGGAAC CATTTTGACC 
ACAATGCCTG TGATGATGGG GCAAGAGAAA GTGCCCATCA AGCAGGTGCC TGGGGGAGTC 
AAGCAGCTGG AGCCCCCCAA AGAAGGAGAG AGGCGGACGA CACACAACAT CATTGAGAAG 
CGGTATCGTT CCTCTATTAA TGACAAAATC ATTGAGTTGA AGGACCTGGT CATGGGGACA 
GATGCCAAGA TGCACAAGTC TGGTGTTCTG AGGAAGGCCA TTGACTACAT CAAGTACTTG 
CAGCAAGTCA ATCATAAACT ACGCCAGGAG AACATGGTGC TGAAGCTGGC AAATCAGAAA 
AACAAGCTCC TGAAGGGCAT CGACCTAGGC AGCCTGGTGG ACAATGATGT GGAGCTAAAG 
ATTGAAGACT TCAATCAGAA TATCCTTCTG ATGTCCCCTC CGGCCTCTGA CTCAGGGTCC 
CAGGCTGGTT TCTCCCCCTA CTCCATCGAC TCCGAGCCAG GAAGCCCTCT GTTGGATGAT 
GCAAAGGTCA AAGATGAGCC AGACTCTCCT CCCGTGGCGC TGGGCATGGT GGACCGCTCT 
CGGATTCTGC TCTGTGTGCT CACCTTCCTC TGCCTCTCCT TTAACCCCCT GACTGCCCTG 
TTGCAGTGGG GAGGGGCCCA CAGCTCTGAC CAGCACCCAT ATTCCGGCTC TGGCCGCAGC 
ATCCTGTCAC TTGAGTCAGG TTCTGGGGGC TGGTTTGATT GGATGATGCC AACGCTTCTC 
TTATGGCTGA TAAATGGTGT GATTGTCCTG AGCGTCTTTG TGAAGCTGCT GGTCCATGGA 
GAGCCAGTGA TCCGGCCACA TTCACGCTCC TCAGTCACCT TCTGGAGACA CCGGAAGCAG 
GCCGACCTGG ACCTCGCCAG GGGGGATTTT GCAGCTGCTG CAGCCAACCT GCAAACCTGC 
CTGTCGGTGT TGGGCCGCAC ACTGCCCACC TCCCGCCTGG ACCTGGCCTG CAGCCTCTCC 
TGGAACGTGA TCCGCTACAG CCTGCAGAAG CTGCGCCTGG TGCGCTGGCT GCTCAAGAAG 
GTCTTCCAGC ACCGGCGGGC TGCCCCGGCC ACCACCGCAG GCTTTGAGGA CGAGGCGAAG 
GCCAGCGCCC GGGACGCGGC CCTGGCCTAT CACAGGCTGC ACCAGCTGCA CATCACTGGG 
AAGCTTCCTG CGGGATCCGC CTGTTCAGAT GTACACATGG CTTTGTGCGC CGTGAACCTG 
GCGGAATGTG CAGAGGAGAA AATCCCACCG AGCACGTTGG TTGAGATCCA TCTGACTGCC 
GCCATGGGGC TCAAGACCCA GTGTGGAGGC AAGCTTGGCT TCCTGGCGAG CTACTTCCTC 
AGTAGAGCCC AGAGCCTGTG TGGCCCGGAG CGCAGCGCCG TCCCTGACTC ACTGCGCTGG 
CTGTGCCACC CCCTGGGCCA GAAGTTTTTC ATGGAGCGGA GCTGGTCGGT GAAGTCAGCT 
GCCAAGGAGA GTCTCTACTG TGCTCAGAGG AACCCAGCTG ACCCCATCGC ACAGGTCCAC 
CAGGCCTTCT GCAAGAACCT ACTGGAGCGA GCAGTTGAAT CACTGGTGAA GCCTCAGGCC 
AAGAAGAAGG CTGGAGACCA GGAAGAAGAG AGCTGCGAAT TCTCTAATGC TCTGGAGTAC 
CTGAAATTAC TGAATTCTTT TGTGGACTCT GTGGGGATTG TGACCCCGCC CTTCTCCAGC 
AGCTCCGTGC TCAAGTCGGC CCTTGGTCCA GATGTCATCT GTCGGTGGTG GACATCCGCC 
ATCACTGTGG CCATCAGCTG GCTCCAGGGA GACGACGCAG CTGTACGCAC TCATTTTACC 
GAAGTAGAGC GTGTCCCCAA AGCCCTGGAA GTAACAGAGA GCCCCCTGGT GAAGGCCGTC 
TTCCACACCT GCAGAGCCAT GCACACCTCA CTCTTGGGGA AGGCGGATGG GCAGCAGAGT 
TCCTTCTGCC ACTGTGAGAG GGCCAGCGGC CACCTGTGGA GCAGCCTCAA CGTTAGCGGG 
GCCACCTGCG ACCTCACCCT CAACCACGTG GTCCAGCTCC TGACCTGTGA CCTGCTACTG 
TCACTGAGGA CAGCACTTTG GCAAAAGCAG GCAGGCGCCA GCCAGGCTCT GGGCGAGACG 
TACCACGCAT CAGGTGCTGA ACTGGCTGGC TTCCAGCGGG ACCTGGGCAG CCTACGCAGG 
CTGGCTCACG GCTTCCGCCC AGCATACCGC AAGGTGTTCC TGCATGAAGC CACTGTGCGC 
CTGATGGCAG GAGCCAGCCC CACCCGCACC CACCAGCTAC TGGAGCATAG TCTGCGCCGG 
CGGACTGCTC AGAGCACCAA GCACGGAGAG GTGGACACCT GGCCCGGACA GCGAGAGCGG 
GCCACCGCCA TCCTGCTGGC CTGCCGCCAC CTTCCCCTGT CCTTCCTGTC CTCCCCCGGC 
CAGCGGGCGG TGCTGCTGGC TGAGGCGGCC CGCACCCTGG AGAAGGCGGG TGACCGACGC 
TCCTGCAACG ACTGCCAGCA GATGATTGTC AAGTTGGGGG GCGGCACGGC CATCGCCGCC 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC481228 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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