ORF » Species Summary » Canis lupus familiaris » GPC3 cDNA ORF clone

GPC3 cDNA ORF clone, Canis lupus familiaris(dog)

  • Gene

  • Clones

  • gRNAs

The following GPC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe32936 XM_538178.6
Latest version!		 Canis lupus familiaris glypican 3 (GPC3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OCe285283 XM_022415889.1
Latest version!		 Canis lupus familiaris glypican 3 (GPC3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OCe32936 XM_538178.5 Canis lupus familiaris glypican 3 (GPC3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    GPC3 ( XM_538178.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_538178.2 Canis lupus familiaris glypican 3 (GPC3), mRNA.
    Price & Availability↑
    CloneID OCe32936
    Clone ID Related Accession (Same CDS sequence) XM_538178.5 , XM_538178.6
    Accession Version XM_538178.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product glypican-3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538178.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGGGA CCGTGCGCAC CGCGTGCTTG GTGGTGGCGA TGCTGCTCAG CCTGGACTGC 
    CCGGGACAGG CGCAGCCCCC GCCGCCGCCG CCGGACGCCA CCTGTCACCA GGTCCGCTCC 
    TTCTTCCAGA GACTGCAGCC CGGACTCAAG TGGGTGCCCG AAACCCCCGT GCCAGGATCA 
    GATTTGCAAG TGTGTCTCCC CAAGGGCCCA ACATGCTGCT CAAGAAAGAT GGAAGAGAAA 
    TACCAGCTGA CAGCACGGTT GAATATAGAA CAGCTGCTTC AGTCTGCAAG CATGGAGCTG 
    AAGTTCCTGA TTATCCAGAA TGCCGCAGTC TTCCAAGAGG CCTTTGAAAT TGTCGTTCGC 
    CATGCTAAGA ACTACACCAA TGCCATGTTC AAGAACAACT ACCCAAGCCT GACCCCACAA 
    GCTTTTGAGT TTGTGGGTGA ATTTTTCACT GACGTGTCTC TCTATATCCT GGGTTCGGAT 
    ATCAATGTGG ATGACATGGT CAATGAGTTG TTCGACAGCC TCTTTCCAGT CATCTACACC 
    CAGCTCATGA ACCCCGGCCT GCCTGAGTCA GCCTTAGACA TCAATGAGTG CCTCCGAGGG 
    GCAAGGCGTG ACCTGAAAGT ATTTGGCAGT TTCCCCAAGC TTATTATGAC CCAGGTTTCC 
    AAGTCACTGC AAGTCACCAG GATATTCCTC CAGGCCCTGA ATCTTGGAAT TGAGGTGATC 
    AACACAACTG ATCACCTAAA GTTCAGTAAA GACTGTGGCC GAATGCTGAC CAGAATGTGG 
    TACTGCTCTT ACTGCCAGGG ACTGATGATG GTTAAGCCTT GTGGGGGCTA CTGCAATGTG 
    GTCATGCAAG GCTGTATGGC AGGTGTGGTG GAGATTGACA AGTACTGGAG AGAATACATT 
    CTGTCTCTGG AAGAACTGGT GAATGGCATG TACAGAATCT ACGACATGGA GAATGTACTG 
    CTTGGTCTCT TTTCGACAAT CCACGACTCC ATTCAGTATG TCCAGAAGAA TGGTGGAAAG 
    CTGACCACCA CGATTGGCAA GTTATGTGCC CATTCTCAAC AACGCCAGTA TAGATCTGCT 
    TATTATCCTG AAGATCTGTT TATTGACAAG AAGGTATTAA AAGTTGCTCA TGTAGAACAT 
    GAAGAAACCT TATCTAGCCG AAGAAGGGAA CTAATTCAGA AGTTGAAATC TTTCATCAGT 
    TTCTACAGTG CTTTGCCTGG CTACGTCTGC AGCCATAGCC CTGTGGCCGA AAACGACACT 
    CTTTGCTGGA ATGGACAAGA ACTCGTGGAG AGATACAGCC AGAAGGCAGC GAGGAATGGC 
    ATGAAAAATC AGTTCAACCT CCATGAGCTG AAAATGAAGG GCCCTGAGCC AGTGGTCAGT 
    CAGATTATTG ACAAACTGAA GCACATTAAC CAGCTCCTGA GAACCATGTC TGTGCCCAAA 
    GGTAGAGTTC TGGATAAAAA CCTTGACGAG GAGGGGCTTG AAAGTGGCGA CTGTGGCGAC 
    GATGAAGACG AGTGCATCGG GGGCTCCGGG GATGGCACGA TGAAAGTGAG GAACCAGCTC 
    CGCTTCCTTG CAGAACTGGC TTATGATCTG GATGTGGATG ATGCTCCTGG AAGCAAGCAG 
    CACGTGAACC AGAAGGACAA TGAGATAGCA GCCTCTCACA ACCTCGGGAA TGGCCATTCC 
    CCCCTGAAGG CCCTTACCAG CTTGGCAGTC TCCGTGGCGT GCTTTTTCTT CCTGGCGCAC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538178.2
    CDS1..1743
    Translation

    Target ORF information:

    RefSeq Version XM_538178.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris glypican 3 (GPC3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_538178.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGGGA CCGTGCGCAC CGCGTGCTTG GTGGTGGCGA TGCTGCTCAG CCTGGACTGC 
    CCGGGACAGG CGCAGCCCCC GCCGCCGCCG CCGGACGCCA CCTGTCACCA GGTCCGCTCC 
    TTCTTCCAGA GACTGCAGCC CGGACTCAAG TGGGTGCCCG AAACCCCCGT GCCAGGATCA 
    GATTTGCAAG TGTGTCTCCC CAAGGGCCCA ACATGCTGCT CAAGAAAGAT GGAAGAGAAA 
    TACCAGCTGA CAGCACGGTT GAATATAGAA CAGCTGCTTC AGTCTGCAAG CATGGAGCTG 
    AAGTTCCTGA TTATCCAGAA TGCCGCAGTC TTCCAAGAGG CCTTTGAAAT TGTCGTTCGC 
    CATGCTAAGA ACTACACCAA TGCCATGTTC AAGAACAACT ACCCAAGCCT GACCCCACAA 
    GCTTTTGAGT TTGTGGGTGA ATTTTTCACT GACGTGTCTC TCTATATCCT GGGTTCGGAT 
    ATCAATGTGG ATGACATGGT CAATGAGTTG TTCGACAGCC TCTTTCCAGT CATCTACACC 
    CAGCTCATGA ACCCCGGCCT GCCTGAGTCA GCCTTAGACA TCAATGAGTG CCTCCGAGGG 
    GCAAGGCGTG ACCTGAAAGT ATTTGGCAGT TTCCCCAAGC TTATTATGAC CCAGGTTTCC 
    AAGTCACTGC AAGTCACCAG GATATTCCTC CAGGCCCTGA ATCTTGGAAT TGAGGTGATC 
    AACACAACTG ATCACCTAAA GTTCAGTAAA GACTGTGGCC GAATGCTGAC CAGAATGTGG 
    TACTGCTCTT ACTGCCAGGG ACTGATGATG GTTAAGCCTT GTGGGGGCTA CTGCAATGTG 
    GTCATGCAAG GCTGTATGGC AGGTGTGGTG GAGATTGACA AGTACTGGAG AGAATACATT 
    CTGTCTCTGG AAGAACTGGT GAATGGCATG TACAGAATCT ACGACATGGA GAATGTACTG 
    CTTGGTCTCT TTTCGACAAT CCACGACTCC ATTCAGTATG TCCAGAAGAA TGGTGGAAAG 
    CTGACCACCA CGATTGGCAA GTTATGTGCC CATTCTCAAC AACGCCAGTA TAGATCTGCT 
    TATTATCCTG AAGATCTGTT TATTGACAAG AAGGTATTAA AAGTTGCTCA TGTAGAACAT 
    GAAGAAACCT TATCTAGCCG AAGAAGGGAA CTAATTCAGA AGTTGAAATC TTTCATCAGT 
    TTCTACAGTG CTTTGCCTGG CTACGTCTGC AGCCATAGCC CTGTGGCCGA AAACGACACT 
    CTTTGCTGGA ATGGACAAGA ACTCGTGGAG AGATACAGCC AGAAGGCAGC GAGGAATGGC 
    ATGAAAAATC AGTTCAACCT CCATGAGCTG AAAATGAAGG GCCCTGAGCC AGTGGTCAGT 
    CAGATTATTG ACAAACTGAA GCACATTAAC CAGCTCCTGA GAACCATGTC TGTGCCCAAA 
    GGTAGAGTTC TGGATAAAAA CCTTGACGAG GAGGGGCTTG AAAGTGGCGA CTGTGGCGAC 
    GATGAAGACG AGTGCATCGG GGGCTCCGGG GATGGCACGA TGAAAGTGAG GAACCAGCTC 
    CGCTTCCTTG CAGAACTGGC TTATGATCTG GATGTGGATG ATGCTCCTGG AAGCAAGCAG 
    CACGTGAACC AGAAGGACAA TGAGATAGCA GCCTCTCACA ACCTCGGGAA TGGCCATTCC 
    CCCCTGAAGG CCCTTACCAG CTTGGCAGTC TCCGTGGCGT GCTTTTTCTT CCTGGCGCAC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    GPC3 ( XM_538178.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_538178.2 Canis lupus familiaris glypican 3 (GPC3), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OCe32936
    Clone ID Related Accession (Same CDS sequence) XM_538178.5 , XM_538178.6
    Accession Version XM_538178.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glypican-3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538178.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGGGA CCGTGCGCAC CGCGTGCTTG GTGGTGGCGA TGCTGCTCAG CCTGGACTGC 
    CCGGGACAGG CGCAGCCCCC GCCGCCGCCG CCGGACGCCA CCTGTCACCA GGTCCGCTCC 
    TTCTTCCAGA GACTGCAGCC CGGACTCAAG TGGGTGCCCG AAACCCCCGT GCCAGGATCA 
    GATTTGCAAG TGTGTCTCCC CAAGGGCCCA ACATGCTGCT CAAGAAAGAT GGAAGAGAAA 
    TACCAGCTGA CAGCACGGTT GAATATAGAA CAGCTGCTTC AGTCTGCAAG CATGGAGCTG 
    AAGTTCCTGA TTATCCAGAA TGCCGCAGTC TTCCAAGAGG CCTTTGAAAT TGTCGTTCGC 
    CATGCTAAGA ACTACACCAA TGCCATGTTC AAGAACAACT ACCCAAGCCT GACCCCACAA 
    GCTTTTGAGT TTGTGGGTGA ATTTTTCACT GACGTGTCTC TCTATATCCT GGGTTCGGAT 
    ATCAATGTGG ATGACATGGT CAATGAGTTG TTCGACAGCC TCTTTCCAGT CATCTACACC 
    CAGCTCATGA ACCCCGGCCT GCCTGAGTCA GCCTTAGACA TCAATGAGTG CCTCCGAGGG 
    GCAAGGCGTG ACCTGAAAGT ATTTGGCAGT TTCCCCAAGC TTATTATGAC CCAGGTTTCC 
    AAGTCACTGC AAGTCACCAG GATATTCCTC CAGGCCCTGA ATCTTGGAAT TGAGGTGATC 
    AACACAACTG ATCACCTAAA GTTCAGTAAA GACTGTGGCC GAATGCTGAC CAGAATGTGG 
    TACTGCTCTT ACTGCCAGGG ACTGATGATG GTTAAGCCTT GTGGGGGCTA CTGCAATGTG 
    GTCATGCAAG GCTGTATGGC AGGTGTGGTG GAGATTGACA AGTACTGGAG AGAATACATT 
    CTGTCTCTGG AAGAACTGGT GAATGGCATG TACAGAATCT ACGACATGGA GAATGTACTG 
    CTTGGTCTCT TTTCGACAAT CCACGACTCC ATTCAGTATG TCCAGAAGAA TGGTGGAAAG 
    CTGACCACCA CGATTGGCAA GTTATGTGCC CATTCTCAAC AACGCCAGTA TAGATCTGCT 
    TATTATCCTG AAGATCTGTT TATTGACAAG AAGGTATTAA AAGTTGCTCA TGTAGAACAT 
    GAAGAAACCT TATCTAGCCG AAGAAGGGAA CTAATTCAGA AGTTGAAATC TTTCATCAGT 
    TTCTACAGTG CTTTGCCTGG CTACGTCTGC AGCCATAGCC CTGTGGCCGA AAACGACACT 
    CTTTGCTGGA ATGGACAAGA ACTCGTGGAG AGATACAGCC AGAAGGCAGC GAGGAATGGC 
    ATGAAAAATC AGTTCAACCT CCATGAGCTG AAAATGAAGG GCCCTGAGCC AGTGGTCAGT 
    CAGATTATTG ACAAACTGAA GCACATTAAC CAGCTCCTGA GAACCATGTC TGTGCCCAAA 
    GGTAGAGTTC TGGATAAAAA CCTTGACGAG GAGGGGCTTG AAAGTGGCGA CTGTGGCGAC 
    GATGAAGACG AGTGCATCGG GGGCTCCGGG GATGGCACGA TGAAAGTGAG GAACCAGCTC 
    CGCTTCCTTG CAGAACTGGC TTATGATCTG GATGTGGATG ATGCTCCTGG AAGCAAGCAG 
    CACGTGAACC AGAAGGACAA TGAGATAGCA GCCTCTCACA ACCTCGGGAA TGGCCATTCC 
    CCCCTGAAGG CCCTTACCAG CTTGGCAGTC TCCGTGGCGT GCTTTTTCTT CCTGGCGCAC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_538178.2
    CDS1..1743
    Translation

    Target ORF information:

    RefSeq Version XM_538178.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glypican 3 (GPC3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_538178.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCGGGGA CCGTGCGCAC CGCGTGCTTG GTGGTGGCGA TGCTGCTCAG CCTGGACTGC 
    CCGGGACAGG CGCAGCCCCC GCCGCCGCCG CCGGACGCCA CCTGTCACCA GGTCCGCTCC 
    TTCTTCCAGA GACTGCAGCC CGGACTCAAG TGGGTGCCCG AAACCCCCGT GCCAGGATCA 
    GATTTGCAAG TGTGTCTCCC CAAGGGCCCA ACATGCTGCT CAAGAAAGAT GGAAGAGAAA 
    TACCAGCTGA CAGCACGGTT GAATATAGAA CAGCTGCTTC AGTCTGCAAG CATGGAGCTG 
    AAGTTCCTGA TTATCCAGAA TGCCGCAGTC TTCCAAGAGG CCTTTGAAAT TGTCGTTCGC 
    CATGCTAAGA ACTACACCAA TGCCATGTTC AAGAACAACT ACCCAAGCCT GACCCCACAA 
    GCTTTTGAGT TTGTGGGTGA ATTTTTCACT GACGTGTCTC TCTATATCCT GGGTTCGGAT 
    ATCAATGTGG ATGACATGGT CAATGAGTTG TTCGACAGCC TCTTTCCAGT CATCTACACC 
    CAGCTCATGA ACCCCGGCCT GCCTGAGTCA GCCTTAGACA TCAATGAGTG CCTCCGAGGG 
    GCAAGGCGTG ACCTGAAAGT ATTTGGCAGT TTCCCCAAGC TTATTATGAC CCAGGTTTCC 
    AAGTCACTGC AAGTCACCAG GATATTCCTC CAGGCCCTGA ATCTTGGAAT TGAGGTGATC 
    AACACAACTG ATCACCTAAA GTTCAGTAAA GACTGTGGCC GAATGCTGAC CAGAATGTGG 
    TACTGCTCTT ACTGCCAGGG ACTGATGATG GTTAAGCCTT GTGGGGGCTA CTGCAATGTG 
    GTCATGCAAG GCTGTATGGC AGGTGTGGTG GAGATTGACA AGTACTGGAG AGAATACATT 
    CTGTCTCTGG AAGAACTGGT GAATGGCATG TACAGAATCT ACGACATGGA GAATGTACTG 
    CTTGGTCTCT TTTCGACAAT CCACGACTCC ATTCAGTATG TCCAGAAGAA TGGTGGAAAG 
    CTGACCACCA CGATTGGCAA GTTATGTGCC CATTCTCAAC AACGCCAGTA TAGATCTGCT 
    TATTATCCTG AAGATCTGTT TATTGACAAG AAGGTATTAA AAGTTGCTCA TGTAGAACAT 
    GAAGAAACCT TATCTAGCCG AAGAAGGGAA CTAATTCAGA AGTTGAAATC TTTCATCAGT 
    TTCTACAGTG CTTTGCCTGG CTACGTCTGC AGCCATAGCC CTGTGGCCGA AAACGACACT 
    CTTTGCTGGA ATGGACAAGA ACTCGTGGAG AGATACAGCC AGAAGGCAGC GAGGAATGGC 
    ATGAAAAATC AGTTCAACCT CCATGAGCTG AAAATGAAGG GCCCTGAGCC AGTGGTCAGT 
    CAGATTATTG ACAAACTGAA GCACATTAAC CAGCTCCTGA GAACCATGTC TGTGCCCAAA 
    GGTAGAGTTC TGGATAAAAA CCTTGACGAG GAGGGGCTTG AAAGTGGCGA CTGTGGCGAC 
    GATGAAGACG AGTGCATCGG GGGCTCCGGG GATGGCACGA TGAAAGTGAG GAACCAGCTC 
    CGCTTCCTTG CAGAACTGGC TTATGATCTG GATGTGGATG ATGCTCCTGG AAGCAAGCAG 
    CACGTGAACC AGAAGGACAA TGAGATAGCA GCCTCTCACA ACCTCGGGAA TGGCCATTCC 
    CCCCTGAAGG CCCTTACCAG CTTGGCAGTC TCCGTGGCGT GCTTTTTCTT CCTGGCGCAC 
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    GPC3 ( XM_022415889.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271597.1 Canis lupus familiaris glypican 3 (GPC3), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OCe285283
    Clone ID Related Accession (Same CDS sequence) XM_022415889.1
    Accession Version XM_022415889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product glypican-3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCGGGGA CCGTGCGCAC CGCGTGCTTG GTGGTGGCGA TGCTGCTCAG CCTGGACTGC 
    CCGGGACAGG CGCAGCCCCC GCCGCCGCCG CCGGACGCCA CCTGTCACCA GGTCCGCTCC 
    TTCTTCCAGA GACTGCAGCC CGGACTCAAG TGGGTGCCCG AAACCCCCGT GCCAGGATCA 
    GATTTGCAAG TGTGTCTCCC CAAGGGCCCA ACATGCTGCT CAAGAAAGAT GGAAGAGAAA 
    TACCAGCTGA CAGCACGGTT GAATATAGAA CAGCTGCTTC AGTCTGCAAG CATGGAGCTG 
    AAGTTCCTGA TTATCCAGAA TGCCGCAGTC TTCCAAGGTG CCCATGCTGT GAAACTGCTA 
    CCCCAAGTGT CTTCTGTCTC CTGCCCCAAC ACTACTGCCC TGCCACCCCT TTTCAAGAAA 
    ATACCCTGTG ATCGAGAGGC CTTTGAAATT GTCGTTCGCC ATGCTAAGAA CTACACCAAT 
    GCCATGTTCA AGAACAACTA CCCAAGCCTG ACCCCACAAG CTTTTGAGTT TGTGGGTGAA 
    TTTTTCACTG ACGTGTCTCT CTATATCCTG GGTTCGGATA TCAATGTGGA TGACATGGTC 
    AATGAGTTGT TCGACAGCCT CTTTCCAGTC ATCTACACCC AGCTCATGAA CCCCGGCCTG 
    CCTGAGTCAG CCTTAGACAT CAATGAGTGC CTCCGAGGGG CAAGGCGTGA CCTGAAAGTA 
    TTTGGCAGTT TCCCCAAGCT TATTATGACC CAGGTTTCCA AGTCACTGCA AGTCACCAGG 
    ATATTCCTCC AGGCCCTGAA TCTTGGAATT GAGGTGATCA ACACAACTGA TCACCTAAAG 
    TTCAGTAAAG ACTGTGGCCG AATGCTGACC AGAATGTGGT ACTGCTCTTA CTGCCAGGGA 
    CTGATGATGG TTAAGCCTTG TGGGGGCTAC TGCAATGTGG TCATGCAAGG CTGTATGGCA 
    GGTGTGGTGG AGATTGACAA GTACTGGAGA GAATACATTC TGTCTCTGGA AGAACTGGTG 
    AATGGCATGT ACAGAATCTA CGACATGGAG AATGTACTGC TTGGTCTCTT TTCGACAATC 
    CACGACTCCA TTCAGTATGT CCAGAAGAAT GGTGGAAAGC TGACCACCAC GATTGGCAAG 
    TTATGTGCCC ATTCTCAACA ACGCCAGTAT AGATCTGCTT ATTATCCTGA AGATCTGTTT 
    ATTGACAAGA AGGTATTAAA AGTTGCTCAT GTAGAACATG AAGAAACCTT ATCTAGCCGA 
    AGAAGGGAAC TAATTCAGAA GTTGAAATCT TTCATCAGTT TCTACAGTGC TTTGCCTGGC 
    TACGTCTGCA GCCATAGCCC TGTGGCCGAA AACGACACTC TTTGCTGGAA TGGACAAGAA 
    CTCGTGGAGA GATACAGCCA GAAGGCAGCG AGGAATGGCA TGAAAAATCA GTTCAACCTC 
    CATGAGCTGA AAATGAAGGG CCCTGAGCCA GTGGTCAGTC AGATTATTGA CAAACTGAAG 
    CACATTAACC AGCTCCTGAG AACCATGTCT GTGCCCAAAG GTAGAGTTCT GGATAAAAAC 
    CTTGACGAGG AGGGGCTTGA AAGTGGCGAC TGTGGCGACG ATGAAGACGA GTGCATCGGG 
    GGCTCCGGGG ATGGCACGAT GAAAGTGAGG AACCAGCTCC GCTTCCTTGC AGAACTGGCT 
    TATGATCTGG ATGTGGATGA TGCTCCTGGA AGCAAGCAGC ACGTGAACCA GAAGGACAAT 
    GAGATAGCAG CCTCTCACAA CCTCGGGAAT GGCCATTCCC CCCTGAAGGC CCTTACCAGC 
    TTGGCAGTCT CCGTGGCGTG CTTTTTCTTC CTGGCGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271597.1
    CDS157..1998
    Translation

    Target ORF information:

    RefSeq Version XM_022415889.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris glypican 3 (GPC3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_022415889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCGGGGA CCGTGCGCAC CGCGTGCTTG GTGGTGGCGA TGCTGCTCAG CCTGGACTGC 
    CCGGGACAGG CGCAGCCCCC GCCGCCGCCG CCGGACGCCA CCTGTCACCA GGTCCGCTCC 
    TTCTTCCAGA GACTGCAGCC CGGACTCAAG TGGGTGCCCG AAACCCCCGT GCCAGGATCA 
    GATTTGCAAG TGTGTCTCCC CAAGGGCCCA ACATGCTGCT CAAGAAAGAT GGAAGAGAAA 
    TACCAGCTGA CAGCACGGTT GAATATAGAA CAGCTGCTTC AGTCTGCAAG CATGGAGCTG 
    AAGTTCCTGA TTATCCAGAA TGCCGCAGTC TTCCAAGGTG CCCATGCTGT GAAACTGCTA 
    CCCCAAGTGT CTTCTGTCTC CTGCCCCAAC ACTACTGCCC TGCCACCCCT TTTCAAGAAA 
    ATACCCTGTG ATCGAGAGGC CTTTGAAATT GTCGTTCGCC ATGCTAAGAA CTACACCAAT 
    GCCATGTTCA AGAACAACTA CCCAAGCCTG ACCCCACAAG CTTTTGAGTT TGTGGGTGAA 
    TTTTTCACTG ACGTGTCTCT CTATATCCTG GGTTCGGATA TCAATGTGGA TGACATGGTC 
    AATGAGTTGT TCGACAGCCT CTTTCCAGTC ATCTACACCC AGCTCATGAA CCCCGGCCTG 
    CCTGAGTCAG CCTTAGACAT CAATGAGTGC CTCCGAGGGG CAAGGCGTGA CCTGAAAGTA 
    TTTGGCAGTT TCCCCAAGCT TATTATGACC CAGGTTTCCA AGTCACTGCA AGTCACCAGG 
    ATATTCCTCC AGGCCCTGAA TCTTGGAATT GAGGTGATCA ACACAACTGA TCACCTAAAG 
    TTCAGTAAAG ACTGTGGCCG AATGCTGACC AGAATGTGGT ACTGCTCTTA CTGCCAGGGA 
    CTGATGATGG TTAAGCCTTG TGGGGGCTAC TGCAATGTGG TCATGCAAGG CTGTATGGCA 
    GGTGTGGTGG AGATTGACAA GTACTGGAGA GAATACATTC TGTCTCTGGA AGAACTGGTG 
    AATGGCATGT ACAGAATCTA CGACATGGAG AATGTACTGC TTGGTCTCTT TTCGACAATC 
    CACGACTCCA TTCAGTATGT CCAGAAGAAT GGTGGAAAGC TGACCACCAC GATTGGCAAG 
    TTATGTGCCC ATTCTCAACA ACGCCAGTAT AGATCTGCTT ATTATCCTGA AGATCTGTTT 
    ATTGACAAGA AGGTATTAAA AGTTGCTCAT GTAGAACATG AAGAAACCTT ATCTAGCCGA 
    AGAAGGGAAC TAATTCAGAA GTTGAAATCT TTCATCAGTT TCTACAGTGC TTTGCCTGGC 
    TACGTCTGCA GCCATAGCCC TGTGGCCGAA AACGACACTC TTTGCTGGAA TGGACAAGAA 
    CTCGTGGAGA GATACAGCCA GAAGGCAGCG AGGAATGGCA TGAAAAATCA GTTCAACCTC 
    CATGAGCTGA AAATGAAGGG CCCTGAGCCA GTGGTCAGTC AGATTATTGA CAAACTGAAG 
    CACATTAACC AGCTCCTGAG AACCATGTCT GTGCCCAAAG GTAGAGTTCT GGATAAAAAC 
    CTTGACGAGG AGGGGCTTGA AAGTGGCGAC TGTGGCGACG ATGAAGACGA GTGCATCGGG 
    GGCTCCGGGG ATGGCACGAT GAAAGTGAGG AACCAGCTCC GCTTCCTTGC AGAACTGGCT 
    TATGATCTGG ATGTGGATGA TGCTCCTGGA AGCAAGCAGC ACGTGAACCA GAAGGACAAT 
    GAGATAGCAG CCTCTCACAA CCTCGGGAAT GGCCATTCCC CCCTGAAGGC CCTTACCAGC 
    TTGGCAGTCT CCGTGGCGTG CTTTTTCTTC CTGGCGCACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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