IGSF1 cDNA ORF clone, Canis lupus familiaris(dog)

The following IGSF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IGSF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe45779 XM_014111979.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OCe285378 XM_022416066.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe285379 XM_022416067.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OCe285380 XM_022416068.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe32915 XM_022416065.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe32915 XM_022416063.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe32915 XM_022416064.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe32915 XM_022416062.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45777 XM_014111977.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45777 XM_014111976.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45777 XM_014111975.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OCe45778 XM_014111978.1
Latest version!
Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe45779
    Clone ID Related Accession (Same CDS sequence) XM_014111979.1
    Accession Version XM_014111979.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product immunoglobulin superfamily member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGTG GGTGTAGCTA TGGCTGTTGG GGTCTGATGA TCGTTGTCCC TGGGATCATG
    GCTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967454.1
    CDS250..978
    Translation

    Target ORF information:

    RefSeq Version XM_014111979.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111979.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGTG GGTGTAGCTA TGGCTGTTGG GGTCTGATGA TCGTTGTCCC TGGGATCATG
    GCTGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285378
    Clone ID Related Accession (Same CDS sequence) XM_022416066.1
    Accession Version XM_022416066.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGGTC TTTACAGGTG CTGCTACTGG
    AAGGAAACAG GCTGGTCAGA GCCCAGTAAA GTTTTAGAGT TGGAGGCACC AGGCCAGCTG
    CCCAAGCCCA TCTTCTGGAT CCAGGCTGAG ACCTACCCTC TTACTGGATG TAATGTTAAC
    ATACTCTGCC ATGGCTGGCT GCAGGATTTG GTATTCATGC TGTTCAAAGA GGGATATGCA
    GAGCCTGTGG ATTACCAAAT CCCAACTGGG ACAGTGGCCA TATTCTCCAT CGCCAACATG
    ACACCTGAGA GTGAAGGGGT TTACATCTGC CGCACTCATA TCCAGATGCT CCCCACTCTG
    TGGTCAGAGC CCAGCAACCC CCTGAAGCTG ATTGTGGCAG GACTCTATCC CAAACCAACT
    CTGACAGCAT ATCCTGGGCC CATTATGGCA CCTGGAGAAA GCCTGAACCT CAGGTGTCAG
    GCACCAATCT ACGGAATGAC CTTTGCTCTA ATAAGGCTTG AAGATTTGAA GAAATCCTTT
    TACCGCAAAA GGCCAATAAA AAATGAGGCA TATTTCTTCT TCCGGGCTTT GAAAATCCAT
    GATGCTGGAC ATTACCTCTG TTTTTACTAT GATGAGTCAT ACAGGGGTTC ACTCCTTAGT
    GATATCCTGA AAATCTGGGT GACCGACACT TTCCCCAAGA CCTGGCTACT TGCTCAGCCC
    AGTCCTGTGG TCCAGATGGG TCAGAACGTG AGCCTGTGGT GTCGAGGGCC AGTGGATGGA
    GTGGGGCTTG CACTCTATAA GAAAGGAGAA GACAAACCAC TTCAGCTTTT GGATACCACC
    AGCATTGATG ACGAGTCATT CTTCCTCAAC AATGTGACCT ACAGTGATGC TGGCATCTAT
    AGTTGCCACT ATCTCCTCTC CTGGAAGACC TCCATCAGGA TGACATCACA CAACACTGTG
    GAACTTGTGG TTGTAGATAA GCCCCCCAAA CCCTCCCTTT CAGCCTGGCC CAGCACCATG
    TTCAAGCTAA GAAAGGCCAT TACCCTTCAG CACCGAGTAC CCCATCCAGT ACTTGAATTT
    TCTCTGGAAT GGGAAGAAAG AGCAACATTC CAAAAATTCT TGGTAGATGG AGATTTCATC
    ATCAGTAATG CTGAAGGAAA AGGCACAGAA ACTTACAGTT GCAGCTATCG CATAGAGGCA
    CACCCCAACA TCTGGTCAGA TCGCAGTGAG CCTCTGAAGC TGATGGGGCC AGCAGGCTTT
    CTTACCTGGA ATTACATTCT GAATGAAGCT ATCAGATTGT CCCTAATCAT GCAGCTTGTT
    GCCTTGCTGT TGGTAGTGCT GTGGATAAGG TGGAAGTGTC GGAGACTCAG AATCAGAGAA
    GCCTGGTTGC TGGGAACAGC TCAAGGGGTC ACCATGCTCT TCATAGTCAC GGCCCTTCTC
    TGCTGTGGAC TGTGCAATGG GGTATTGACA GAAGAGACTG AAATAATCAT GCCAACCCCT
    AAGCCTGAGC TGTGGGCAGA GACCAACTTC CCTCTTGCCC CGTGGAAGAA CTTAACCCTC
    TGGTGCAGAA GCCCTTCTGG CTCAACTAAG GAGTTTGTGT TGCTGAAGGA CGGGACTGGG
    TGGATTGCAA CTCGCCCGGC CTCCGAGCAG GTCCGGGCTG CCTTTCCCCT TGGCGCCCTA
    ACCCAGAGCC ATACCGGGAG TTACCACTGC CATTCATGGG AGGAGATGGC TGTGTCGGAG
    CCCAGTGAGG CACTTGAGCT GGTGGGGACA GACATCCTCC CCAAACCTGT CATTTCTGCT
    TCCCCCCCAA TTCGGGGCCA GAAACTGCAA ATCCGGTGCA AAGGATGGCT GGCAGGCATG
    GAGTTCGTTC TGTATAAGGA GGGAGTGCAG GAACCTGTCC AGCAACTTCA TGCCGTTGGG
    AGAGAAGCCT TCTTTACAAT CCAAAGAATG GAGGATAAAG ACGAAGGCAA TTATAGCTGC
    CGTACTCATA CTGAAAAGCG CCCCTTCAAG TGGTCTGAGC CCAGTGATCC CATGGAGCTT
    GTCATAAAAG AAATGTACCC CAAGCCCTTT TTCAAGACAT GGGCCAGCCC TGTGGTCACT
    CCTGGTGCCC GAGTGACTTT CAATTGCTCC ACTCCCCAGC AGCACATGAG CTTTATTCTT
    TACAAAGATG GAAGTGAAAT AGCATCCAGT GACAGGTCTT GGGCAAGTCC AGGGGCCAGT
    GCGGCTCACT TTCTGATCAT TTCAGTGGGC ACTGGTGATG GAGGGAATTA TAGCTGCCGC
    TATTATGACT TCGCTATCTG GTCTGAGCCC AGCGACCCTG TGGAGCTCGT GGTGACAGAA
    TTCTACCCCA AACCTACTCT TCTGGCACAC CCGGGTCCTG TGGTGCTTCC TGGAAAGAAT
    GTGACCCTGC GCTGCCAAGG GGCTTTCCAA GGCATGAGAT TTGCCCTCTT GCAGGAGGGA
    ACCCAGGTTC CCTTACAGTT CCAGAGCACC TCCGGGAATT CAGCTGACTT CCTCCTCCAC
    ACTGTTAGAG CAGAGGACTC TGGGAACTAC AGTTGTGTTT ACTACGAGAC AACCATGTCA
    AATAGGGGAT CACATCTCAG CAAGCCCATT ATGATCTGGG TGACTGACAC ATTTCCCAAG
    CCATGGTTGT TTGCTGAGCC CAGTTCTGTG GTTCCTATGG GGCAGAATGT TACTCTCTGG
    TGCCAAGGGC CAGTCCATGG AGTAGGATAC ATTCTGCACA AAGAAAGAGA AACCACTTCA
    GTGCAGCTCT GGGGATCCAC CAGTAATGAT GGGGCATTCC CTATCACCAA TATATCTGGT
    GCTAACATAG GGCGTTACAG CTGCTGCTAC CACCCTGACT GGACCAGCCC TATCAAGATA
    CAGCCTAGCA ACACTCTGGA ACTCATAGTC ACAGGTTTGC TCCCCAAACC CAGCCTCTTA
    GCCCAGCCAG GTCCTATTGT GGCCCCTGGA GAAAATATGA CTTTTCAATG CCAAGGGGAA
    CTACCAGACT CAACATTTGT CCTGTTGAAG GAAGGCACTC AACAGCCTTT GGAACAACAG
    AGCCCAAGTG GGTACAGGGC TGACTTCTGG ATGCCAGCAG TGAGAGGTGA TGATTCTGGG
    GTCTATAGCT GTGTTTATTA TTTGGACTCT GCTCCCTTTG CGGCTTCTAA TCACAGTGAC
    TTCCTGGAGA TCTGGGTGAC TGATAAGCCC CCTAAGCCCT CGCTGTCAGC CTGGCCCAGC
    ACCATGTTCA AGCTAGGCAA GGACATCACC CTTCAGTGCC GTGGCCCCCT GCCAGGTGTT
    GAATTTGTCC TGGAACATGA TGGAGAAGAA GCACCTCAGC AGTTCTCAGA GGATGGGGAC
    TTTGTCATCA ACAATGTAGA AGGAAAAGGC ATTGGCAACT ACAGCTGCAG CTACCGCCTC
    CAGGCCTATC CTGATATCTG GTCAGAGCCT AGTGATGCCC TGGAACTGGT GGGGGCAGCA
    GGGCCTGCTG CCCAGGAGTG CACTGTGGGG AACATTGTCC GAAGTAGTCT GATTGTGGTG
    GTAGTTGTAG CTTTGGGGGT AGTGCTAGCC ATAGAATGGA AGAAGTGGCC TCGACTCCGA
    ACCAGGGACT CAGAGACAGA TGGAAGAGAC CAGACCATAG CCCTTGAAGA GTGTAACCAA
    GAAGGAGAAC CAGCCACCAA CACCAGCTCT CCCTCCTCCG TCTCTCAGGG AACCTCAGTG
    GAACTGCCAG TCCCAGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271774.1
    CDS199..3999
    Translation

    Target ORF information:

    RefSeq Version XM_022416066.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416066.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGGTC TTTACAGGTG CTGCTACTGG
    AAGGAAACAG GCTGGTCAGA GCCCAGTAAA GTTTTAGAGT TGGAGGCACC AGGCCAGCTG
    CCCAAGCCCA TCTTCTGGAT CCAGGCTGAG ACCTACCCTC TTACTGGATG TAATGTTAAC
    ATACTCTGCC ATGGCTGGCT GCAGGATTTG GTATTCATGC TGTTCAAAGA GGGATATGCA
    GAGCCTGTGG ATTACCAAAT CCCAACTGGG ACAGTGGCCA TATTCTCCAT CGCCAACATG
    ACACCTGAGA GTGAAGGGGT TTACATCTGC CGCACTCATA TCCAGATGCT CCCCACTCTG
    TGGTCAGAGC CCAGCAACCC CCTGAAGCTG ATTGTGGCAG GACTCTATCC CAAACCAACT
    CTGACAGCAT ATCCTGGGCC CATTATGGCA CCTGGAGAAA GCCTGAACCT CAGGTGTCAG
    GCACCAATCT ACGGAATGAC CTTTGCTCTA ATAAGGCTTG AAGATTTGAA GAAATCCTTT
    TACCGCAAAA GGCCAATAAA AAATGAGGCA TATTTCTTCT TCCGGGCTTT GAAAATCCAT
    GATGCTGGAC ATTACCTCTG TTTTTACTAT GATGAGTCAT ACAGGGGTTC ACTCCTTAGT
    GATATCCTGA AAATCTGGGT GACCGACACT TTCCCCAAGA CCTGGCTACT TGCTCAGCCC
    AGTCCTGTGG TCCAGATGGG TCAGAACGTG AGCCTGTGGT GTCGAGGGCC AGTGGATGGA
    GTGGGGCTTG CACTCTATAA GAAAGGAGAA GACAAACCAC TTCAGCTTTT GGATACCACC
    AGCATTGATG ACGAGTCATT CTTCCTCAAC AATGTGACCT ACAGTGATGC TGGCATCTAT
    AGTTGCCACT ATCTCCTCTC CTGGAAGACC TCCATCAGGA TGACATCACA CAACACTGTG
    GAACTTGTGG TTGTAGATAA GCCCCCCAAA CCCTCCCTTT CAGCCTGGCC CAGCACCATG
    TTCAAGCTAA GAAAGGCCAT TACCCTTCAG CACCGAGTAC CCCATCCAGT ACTTGAATTT
    TCTCTGGAAT GGGAAGAAAG AGCAACATTC CAAAAATTCT TGGTAGATGG AGATTTCATC
    ATCAGTAATG CTGAAGGAAA AGGCACAGAA ACTTACAGTT GCAGCTATCG CATAGAGGCA
    CACCCCAACA TCTGGTCAGA TCGCAGTGAG CCTCTGAAGC TGATGGGGCC AGCAGGCTTT
    CTTACCTGGA ATTACATTCT GAATGAAGCT ATCAGATTGT CCCTAATCAT GCAGCTTGTT
    GCCTTGCTGT TGGTAGTGCT GTGGATAAGG TGGAAGTGTC GGAGACTCAG AATCAGAGAA
    GCCTGGTTGC TGGGAACAGC TCAAGGGGTC ACCATGCTCT TCATAGTCAC GGCCCTTCTC
    TGCTGTGGAC TGTGCAATGG GGTATTGACA GAAGAGACTG AAATAATCAT GCCAACCCCT
    AAGCCTGAGC TGTGGGCAGA GACCAACTTC CCTCTTGCCC CGTGGAAGAA CTTAACCCTC
    TGGTGCAGAA GCCCTTCTGG CTCAACTAAG GAGTTTGTGT TGCTGAAGGA CGGGACTGGG
    TGGATTGCAA CTCGCCCGGC CTCCGAGCAG GTCCGGGCTG CCTTTCCCCT TGGCGCCCTA
    ACCCAGAGCC ATACCGGGAG TTACCACTGC CATTCATGGG AGGAGATGGC TGTGTCGGAG
    CCCAGTGAGG CACTTGAGCT GGTGGGGACA GACATCCTCC CCAAACCTGT CATTTCTGCT
    TCCCCCCCAA TTCGGGGCCA GAAACTGCAA ATCCGGTGCA AAGGATGGCT GGCAGGCATG
    GAGTTCGTTC TGTATAAGGA GGGAGTGCAG GAACCTGTCC AGCAACTTCA TGCCGTTGGG
    AGAGAAGCCT TCTTTACAAT CCAAAGAATG GAGGATAAAG ACGAAGGCAA TTATAGCTGC
    CGTACTCATA CTGAAAAGCG CCCCTTCAAG TGGTCTGAGC CCAGTGATCC CATGGAGCTT
    GTCATAAAAG AAATGTACCC CAAGCCCTTT TTCAAGACAT GGGCCAGCCC TGTGGTCACT
    CCTGGTGCCC GAGTGACTTT CAATTGCTCC ACTCCCCAGC AGCACATGAG CTTTATTCTT
    TACAAAGATG GAAGTGAAAT AGCATCCAGT GACAGGTCTT GGGCAAGTCC AGGGGCCAGT
    GCGGCTCACT TTCTGATCAT TTCAGTGGGC ACTGGTGATG GAGGGAATTA TAGCTGCCGC
    TATTATGACT TCGCTATCTG GTCTGAGCCC AGCGACCCTG TGGAGCTCGT GGTGACAGAA
    TTCTACCCCA AACCTACTCT TCTGGCACAC CCGGGTCCTG TGGTGCTTCC TGGAAAGAAT
    GTGACCCTGC GCTGCCAAGG GGCTTTCCAA GGCATGAGAT TTGCCCTCTT GCAGGAGGGA
    ACCCAGGTTC CCTTACAGTT CCAGAGCACC TCCGGGAATT CAGCTGACTT CCTCCTCCAC
    ACTGTTAGAG CAGAGGACTC TGGGAACTAC AGTTGTGTTT ACTACGAGAC AACCATGTCA
    AATAGGGGAT CACATCTCAG CAAGCCCATT ATGATCTGGG TGACTGACAC ATTTCCCAAG
    CCATGGTTGT TTGCTGAGCC CAGTTCTGTG GTTCCTATGG GGCAGAATGT TACTCTCTGG
    TGCCAAGGGC CAGTCCATGG AGTAGGATAC ATTCTGCACA AAGAAAGAGA AACCACTTCA
    GTGCAGCTCT GGGGATCCAC CAGTAATGAT GGGGCATTCC CTATCACCAA TATATCTGGT
    GCTAACATAG GGCGTTACAG CTGCTGCTAC CACCCTGACT GGACCAGCCC TATCAAGATA
    CAGCCTAGCA ACACTCTGGA ACTCATAGTC ACAGGTTTGC TCCCCAAACC CAGCCTCTTA
    GCCCAGCCAG GTCCTATTGT GGCCCCTGGA GAAAATATGA CTTTTCAATG CCAAGGGGAA
    CTACCAGACT CAACATTTGT CCTGTTGAAG GAAGGCACTC AACAGCCTTT GGAACAACAG
    AGCCCAAGTG GGTACAGGGC TGACTTCTGG ATGCCAGCAG TGAGAGGTGA TGATTCTGGG
    GTCTATAGCT GTGTTTATTA TTTGGACTCT GCTCCCTTTG CGGCTTCTAA TCACAGTGAC
    TTCCTGGAGA TCTGGGTGAC TGATAAGCCC CCTAAGCCCT CGCTGTCAGC CTGGCCCAGC
    ACCATGTTCA AGCTAGGCAA GGACATCACC CTTCAGTGCC GTGGCCCCCT GCCAGGTGTT
    GAATTTGTCC TGGAACATGA TGGAGAAGAA GCACCTCAGC AGTTCTCAGA GGATGGGGAC
    TTTGTCATCA ACAATGTAGA AGGAAAAGGC ATTGGCAACT ACAGCTGCAG CTACCGCCTC
    CAGGCCTATC CTGATATCTG GTCAGAGCCT AGTGATGCCC TGGAACTGGT GGGGGCAGCA
    GGGCCTGCTG CCCAGGAGTG CACTGTGGGG AACATTGTCC GAAGTAGTCT GATTGTGGTG
    GTAGTTGTAG CTTTGGGGGT AGTGCTAGCC ATAGAATGGA AGAAGTGGCC TCGACTCCGA
    ACCAGGGACT CAGAGACAGA TGGAAGAGAC CAGACCATAG CCCTTGAAGA GTGTAACCAA
    GAAGGAGAAC CAGCCACCAA CACCAGCTCT CCCTCCTCCG TCTCTCAGGG AACCTCAGTG
    GAACTGCCAG TCCCAGTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285379
    Clone ID Related Accession (Same CDS sequence) XM_022416067.1
    Accession Version XM_022416067.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3684bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGACATCC TCCCCAAACC TGTCATTTCT
    GCTTCCCCCC CAATTCGGGG CCAGAAACTG CAAATCCGGT GCAAAGGATG GCTGGCAGGC
    ATGGAGTTCG TTCTGTATAA GGAGGGAGTG CAGGAACCTG TCCAGCAACT TCATGCCGTT
    GGGAGAGAAG CCTTCTTTAC AATCCAAAGA ATGGAGGATA AAGACGAAGG CAATTATAGC
    TGCCGTACTC ATACTGAAAA GCGCCCCTTC AAGTGGTCTG AGCCCAGTGA TCCCATGGAG
    CTTGTCATAA AAGAAATGTA CCCCAAGCCC TTTTTCAAGA CATGGGCCAG CCCTGTGGTC
    ACTCCTGGTG CCCGAGTGAC TTTCAATTGC TCCACTCCCC AGCAGCACAT GAGCTTTATT
    CTTTACAAAG ATGGAAGTGA AATAGCATCC AGTGACAGGT CTTGGGCAAG TCCAGGGGCC
    AGTGCGGCTC ACTTTCTGAT CATTTCAGTG GGCACTGGTG ATGGAGGGAA TTATAGCTGC
    CGCTATTATG ACTTCGCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA
    GAATTCTACC CCAAACCTAC TCTTCTGGCA CACCCGGGTC CTGTGGTGCT TCCTGGAAAG
    AATGTGACCC TGCGCTGCCA AGGGGCTTTC CAAGGCATGA GATTTGCCCT CTTGCAGGAG
    GGAACCCAGG TTCCCTTACA GTTCCAGAGC ACCTCCGGGA ATTCAGCTGA CTTCCTCCTC
    CACACTGTTA GAGCAGAGGA CTCTGGGAAC TACAGTTGTG TTTACTACGA GACAACCATG
    TCAAATAGGG GATCACATCT CAGCAAGCCC ATTATGATCT GGGTGACTGA CACATTTCCC
    AAGCCATGGT TGTTTGCTGA GCCCAGTTCT GTGGTTCCTA TGGGGCAGAA TGTTACTCTC
    TGGTGCCAAG GGCCAGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAAG AGAAACCACT
    TCAGTGCAGC TCTGGGGATC CACCAGTAAT GATGGGGCAT TCCCTATCAC CAATATATCT
    GGTGCTAACA TAGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG CCCTATCAAG
    ATACAGCCTA GCAACACTCT GGAACTCATA GTCACAGGTT TGCTCCCCAA ACCCAGCCTC
    TTAGCCCAGC CAGGTCCTAT TGTGGCCCCT GGAGAAAATA TGACTTTTCA ATGCCAAGGG
    GAACTACCAG ACTCAACATT TGTCCTGTTG AAGGAAGGCA CTCAACAGCC TTTGGAACAA
    CAGAGCCCAA GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGATGATTCT
    GGGGTCTATA GCTGTGTTTA TTATTTGGAC TCTGCTCCCT TTGCGGCTTC TAATCACAGT
    GACTTCCTGG AGATCTGGGT GACTGATAAG CCCCCTAAGC CCTCGCTGTC AGCCTGGCCC
    AGCACCATGT TCAAGCTAGG CAAGGACATC ACCCTTCAGT GCCGTGGCCC CCTGCCAGGT
    GTTGAATTTG TCCTGGAACA TGATGGAGAA GAAGCACCTC AGCAGTTCTC AGAGGATGGG
    GACTTTGTCA TCAACAATGT AGAAGGAAAA GGCATTGGCA ACTACAGCTG CAGCTACCGC
    CTCCAGGCCT ATCCTGATAT CTGGTCAGAG CCTAGTGATG CCCTGGAACT GGTGGGGGCA
    GCAGGGCCTG CTGCCCAGGA GTGCACTGTG GGGAACATTG TCCGAAGTAG TCTGATTGTG
    GTGGTAGTTG TAGCTTTGGG GGTAGTGCTA GCCATAGAAT GGAAGAAGTG GCCTCGACTC
    CGAACCAGGG ACTCAGAGAC AGATGGAAGA GACCAGACCA TAGCCCTTGA AGAGTGTAAC
    CAAGAAGGAG AACCAGCCAC CAACACCAGC TCTCCCTCCT CCGTCTCTCA GGGAACCTCA
    GTGGAACTGC CAGTCCCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271775.1
    CDS199..3882
    Translation

    Target ORF information:

    RefSeq Version XM_022416067.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416067.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGACATCC TCCCCAAACC TGTCATTTCT
    GCTTCCCCCC CAATTCGGGG CCAGAAACTG CAAATCCGGT GCAAAGGATG GCTGGCAGGC
    ATGGAGTTCG TTCTGTATAA GGAGGGAGTG CAGGAACCTG TCCAGCAACT TCATGCCGTT
    GGGAGAGAAG CCTTCTTTAC AATCCAAAGA ATGGAGGATA AAGACGAAGG CAATTATAGC
    TGCCGTACTC ATACTGAAAA GCGCCCCTTC AAGTGGTCTG AGCCCAGTGA TCCCATGGAG
    CTTGTCATAA AAGAAATGTA CCCCAAGCCC TTTTTCAAGA CATGGGCCAG CCCTGTGGTC
    ACTCCTGGTG CCCGAGTGAC TTTCAATTGC TCCACTCCCC AGCAGCACAT GAGCTTTATT
    CTTTACAAAG ATGGAAGTGA AATAGCATCC AGTGACAGGT CTTGGGCAAG TCCAGGGGCC
    AGTGCGGCTC ACTTTCTGAT CATTTCAGTG GGCACTGGTG ATGGAGGGAA TTATAGCTGC
    CGCTATTATG ACTTCGCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA
    GAATTCTACC CCAAACCTAC TCTTCTGGCA CACCCGGGTC CTGTGGTGCT TCCTGGAAAG
    AATGTGACCC TGCGCTGCCA AGGGGCTTTC CAAGGCATGA GATTTGCCCT CTTGCAGGAG
    GGAACCCAGG TTCCCTTACA GTTCCAGAGC ACCTCCGGGA ATTCAGCTGA CTTCCTCCTC
    CACACTGTTA GAGCAGAGGA CTCTGGGAAC TACAGTTGTG TTTACTACGA GACAACCATG
    TCAAATAGGG GATCACATCT CAGCAAGCCC ATTATGATCT GGGTGACTGA CACATTTCCC
    AAGCCATGGT TGTTTGCTGA GCCCAGTTCT GTGGTTCCTA TGGGGCAGAA TGTTACTCTC
    TGGTGCCAAG GGCCAGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAAG AGAAACCACT
    TCAGTGCAGC TCTGGGGATC CACCAGTAAT GATGGGGCAT TCCCTATCAC CAATATATCT
    GGTGCTAACA TAGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG CCCTATCAAG
    ATACAGCCTA GCAACACTCT GGAACTCATA GTCACAGGTT TGCTCCCCAA ACCCAGCCTC
    TTAGCCCAGC CAGGTCCTAT TGTGGCCCCT GGAGAAAATA TGACTTTTCA ATGCCAAGGG
    GAACTACCAG ACTCAACATT TGTCCTGTTG AAGGAAGGCA CTCAACAGCC TTTGGAACAA
    CAGAGCCCAA GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGATGATTCT
    GGGGTCTATA GCTGTGTTTA TTATTTGGAC TCTGCTCCCT TTGCGGCTTC TAATCACAGT
    GACTTCCTGG AGATCTGGGT GACTGATAAG CCCCCTAAGC CCTCGCTGTC AGCCTGGCCC
    AGCACCATGT TCAAGCTAGG CAAGGACATC ACCCTTCAGT GCCGTGGCCC CCTGCCAGGT
    GTTGAATTTG TCCTGGAACA TGATGGAGAA GAAGCACCTC AGCAGTTCTC AGAGGATGGG
    GACTTTGTCA TCAACAATGT AGAAGGAAAA GGCATTGGCA ACTACAGCTG CAGCTACCGC
    CTCCAGGCCT ATCCTGATAT CTGGTCAGAG CCTAGTGATG CCCTGGAACT GGTGGGGGCA
    GCAGGGCCTG CTGCCCAGGA GTGCACTGTG GGGAACATTG TCCGAAGTAG TCTGATTGTG
    GTGGTAGTTG TAGCTTTGGG GGTAGTGCTA GCCATAGAAT GGAAGAAGTG GCCTCGACTC
    CGAACCAGGG ACTCAGAGAC AGATGGAAGA GACCAGACCA TAGCCCTTGA AGAGTGTAAC
    CAAGAAGGAG AACCAGCCAC CAACACCAGC TCTCCCTCCT CCGTCTCTCA GGGAACCTCA
    GTGGAACTGC CAGTCCCAGT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285380
    Clone ID Related Accession (Same CDS sequence) XM_022416068.1
    Accession Version XM_022416068.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGATAAGCC CCCTAAGCCC TCGCTGTCAG CCTGGCCCAG CACCATGTTC
    AAGCTAGGCA AGGACATCAC CCTTCAGTGC CGTGGCCCCC TGCCAGGTGT TGAATTTGTC
    CTGGAACATG ATGGAGAAGA AGCACCTCAG CAGTTCTCAG AGGATGGGGA CTTTGTCATC
    AACAATGTAG AAGGAAAAGG CATTGGCAAC TACAGCTGCA GCTACCGCCT CCAGGCCTAT
    CCTGATATCT GGTCAGAGCC TAGTGATGCC CTGGAACTGG TGGGGGCAGC AGGGCCTGCT
    GCCCAGGAGT GCACTGTGGG GAACATTGTC CGAAGTAGTC TGATTGTGGT GGTAGTTGTA
    GCTTTGGGGG TAGTGCTAGC CATAGAATGG AAGAAGTGGC CTCGACTCCG AACCAGGGAC
    TCAGAGACAG ATGGAAGAGA CCAGACCATA GCCCTTGAAG AGTGTAACCA AGAAGGAGAA
    CCAGCCACCA ACACCAGCTC TCCCTCCTCC GTCTCTCAGG GAACCTCAGT GGAACTGCCA
    GTCCCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271776.1
    CDS201..3632
    Translation

    Target ORF information:

    RefSeq Version XM_022416068.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416068.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGATAAGCC CCCTAAGCCC TCGCTGTCAG CCTGGCCCAG CACCATGTTC
    AAGCTAGGCA AGGACATCAC CCTTCAGTGC CGTGGCCCCC TGCCAGGTGT TGAATTTGTC
    CTGGAACATG ATGGAGAAGA AGCACCTCAG CAGTTCTCAG AGGATGGGGA CTTTGTCATC
    AACAATGTAG AAGGAAAAGG CATTGGCAAC TACAGCTGCA GCTACCGCCT CCAGGCCTAT
    CCTGATATCT GGTCAGAGCC TAGTGATGCC CTGGAACTGG TGGGGGCAGC AGGGCCTGCT
    GCCCAGGAGT GCACTGTGGG GAACATTGTC CGAAGTAGTC TGATTGTGGT GGTAGTTGTA
    GCTTTGGGGG TAGTGCTAGC CATAGAATGG AAGAAGTGGC CTCGACTCCG AACCAGGGAC
    TCAGAGACAG ATGGAAGAGA CCAGACCATA GCCCTTGAAG AGTGTAACCA AGAAGGAGAA
    CCAGCCACCA ACACCAGCTC TCCCTCCTCC GTCTCTCAGG GAACCTCAGT GGAACTGCCA
    GTCCCAGTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32915
    Clone ID Related Accession (Same CDS sequence) XM_022416062.1 , XM_022416063.1 , XM_022416065.1 , XM_022416064.1
    Accession Version XM_022416062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271770.1
    CDS199..4206
    Translation

    Target ORF information:

    RefSeq Version XM_022416062.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32915
    Clone ID Related Accession (Same CDS sequence) XM_022416062.1 , XM_022416063.1 , XM_022416065.1 , XM_022416064.1
    Accession Version XM_022416063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271771.1
    CDS352..4359
    Translation

    Target ORF information:

    RefSeq Version XM_022416063.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32915
    Clone ID Related Accession (Same CDS sequence) XM_022416062.1 , XM_022416063.1 , XM_022416065.1 , XM_022416064.1
    Accession Version XM_022416065.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271773.1
    CDS334..4341
    Translation

    Target ORF information:

    RefSeq Version XM_022416065.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416065.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32915
    Clone ID Related Accession (Same CDS sequence) XM_022416062.1 , XM_022416063.1 , XM_022416065.1 , XM_022416064.1
    Accession Version XM_022416064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271772.1
    CDS356..4363
    Translation

    Target ORF information:

    RefSeq Version XM_022416064.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris immunoglobulin superfamily member 1 (IGSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022416064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GGCTGAGTCT GGGTATGACA TCAATAGTGA TGCAATCTCA ACCGGAGCTG
    TGGATAGAGA CCAATTACCC CCAGACCCCT TGGGAGAACA TCACACTTTG GTGCAAAAGC
    CCCTCTCGGA TTTCAAGCAA GTTTCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCT CCAAGACCTT CCAAGTTTCA TTCCCTATAG GTGCCCTTAC TAAGTCCAAT
    GCAGGTCTTT ACAGGTGCTG CTACTGGAAG GAAACAGGCT GGTCAGAGCC CAGTAAAGTT
    TTAGAGTTGG AGGCACCAGG CCAGCTGCCC AAGCCCATCT TCTGGATCCA GGCTGAGACC
    TACCCTCTTA CTGGATGTAA TGTTAACATA CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TCAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAATCCC AACTGGGACA
    GTGGCCATAT TCTCCATCGC CAACATGACA CCTGAGAGTG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACTCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGATT
    GTGGCAGGAC TCTATCCCAA ACCAACTCTG ACAGCATATC CTGGGCCCAT TATGGCACCT
    GGAGAAAGCC TGAACCTCAG GTGTCAGGCA CCAATCTACG GAATGACCTT TGCTCTAATA
    AGGCTTGAAG ATTTGAAGAA ATCCTTTTAC CGCAAAAGGC CAATAAAAAA TGAGGCATAT
    TTCTTCTTCC GGGCTTTGAA AATCCATGAT GCTGGACATT ACCTCTGTTT TTACTATGAT
    GAGTCATACA GGGGTTCACT CCTTAGTGAT ATCCTGAAAA TCTGGGTGAC CGACACTTTC
    CCCAAGACCT GGCTACTTGC TCAGCCCAGT CCTGTGGTCC AGATGGGTCA GAACGTGAGC
    CTGTGGTGTC GAGGGCCAGT GGATGGAGTG GGGCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AGCTTTTGGA TACCACCAGC ATTGATGACG AGTCATTCTT CCTCAACAAT
    GTGACCTACA GTGATGCTGG CATCTATAGT TGCCACTATC TCCTCTCCTG GAAGACCTCC
    ATCAGGATGA CATCACACAA CACTGTGGAA CTTGTGGTTG TAGATAAGCC CCCCAAACCC
    TCCCTTTCAG CCTGGCCCAG CACCATGTTC AAGCTAAGAA AGGCCATTAC CCTTCAGCAC
    CGAGTACCCC ATCCAGTACT TGAATTTTCT CTGGAATGGG AAGAAAGAGC AACATTCCAA
    AAATTCTTGG TAGATGGAGA TTTCATCATC AGTAATGCTG AAGGAAAAGG CACAGAAACT
    TACAGTTGCA GCTATCGCAT AGAGGCACAC CCCAACATCT GGTCAGATCG CAGTGAGCCT
    CTGAAGCTGA TGGGGCCAGC AGGCTTTCTT ACCTGGAATT ACATTCTGAA TGAAGCTATC
    AGATTGTCCC TAATCATGCA GCTTGTTGCC TTGCTGTTGG TAGTGCTGTG GATAAGGTGG
    AAGTGTCGGA GACTCAGAAT CAGAGAAGCC TGGTTGCTGG GAACAGCTCA AGGGGTCACC
    ATGCTCTTCA TAGTCACGGC CCTTCTCTGC TGTGGACTGT GCAATGGGGT ATTGACAGAA
    GAGACTGAAA TAATCATGCC AACCCCTAAG CCTGAGCTGT GGGCAGAGAC CAACTTCCCT
    CTTGCCCCGT GGAAGAACTT AACCCTCTGG TGCAGAAGCC CTTCTGGCTC AACTAAGGAG
    TTTGTGTTGC TGAAGGACGG GACTGGGTGG ATTGCAACTC GCCCGGCCTC CGAGCAGGTC
    CGGGCTGCCT TTCCCCTTGG CGCCCTAACC CAGAGCCATA CCGGGAGTTA CCACTGCCAT
    TCATGGGAGG AGATGGCTGT GTCGGAGCCC AGTGAGGCAC TTGAGCTGGT GGGGACAGAC
    ATCCTCCCCA AACCTGTCAT TTCTGCTTCC CCCCCAATTC GGGGCCAGAA ACTGCAAATC
    CGGTGCAAAG GATGGCTGGC AGGCATGGAG TTCGTTCTGT ATAAGGAGGG AGTGCAGGAA
    CCTGTCCAGC AACTTCATGC CGTTGGGAGA GAAGCCTTCT TTACAATCCA AAGAATGGAG
    GATAAAGACG AAGGCAATTA TAGCTGCCGT ACTCATACTG AAAAGCGCCC CTTCAAGTGG
    TCTGAGCCCA GTGATCCCAT GGAGCTTGTC ATAAAAGAAA TGTACCCCAA GCCCTTTTTC
    AAGACATGGG CCAGCCCTGT GGTCACTCCT GGTGCCCGAG TGACTTTCAA TTGCTCCACT
    CCCCAGCAGC ACATGAGCTT TATTCTTTAC AAAGATGGAA GTGAAATAGC ATCCAGTGAC
    AGGTCTTGGG CAAGTCCAGG GGCCAGTGCG GCTCACTTTC TGATCATTTC AGTGGGCACT
    GGTGATGGAG GGAATTATAG CTGCCGCTAT TATGACTTCG CTATCTGGTC TGAGCCCAGC
    GACCCTGTGG AGCTCGTGGT GACAGAATTC TACCCCAAAC CTACTCTTCT GGCACACCCG
    GGTCCTGTGG TGCTTCCTGG AAAGAATGTG ACCCTGCGCT GCCAAGGGGC TTTCCAAGGC
    ATGAGATTTG CCCTCTTGCA GGAGGGAACC CAGGTTCCCT TACAGTTCCA GAGCACCTCC
    GGGAATTCAG CTGACTTCCT CCTCCACACT GTTAGAGCAG AGGACTCTGG GAACTACAGT
    TGTGTTTACT ACGAGACAAC CATGTCAAAT AGGGGATCAC ATCTCAGCAA GCCCATTATG
    ATCTGGGTGA CTGACACATT TCCCAAGCCA TGGTTGTTTG CTGAGCCCAG TTCTGTGGTT
    CCTATGGGGC AGAATGTTAC TCTCTGGTGC CAAGGGCCAG TCCATGGAGT AGGATACATT
    CTGCACAAAG AAAGAGAAAC CACTTCAGTG CAGCTCTGGG GATCCACCAG TAATGATGGG
    GCATTCCCTA TCACCAATAT ATCTGGTGCT AACATAGGGC GTTACAGCTG CTGCTACCAC
    CCTGACTGGA CCAGCCCTAT CAAGATACAG CCTAGCAACA CTCTGGAACT CATAGTCACA
    GGTTTGCTCC CCAAACCCAG CCTCTTAGCC CAGCCAGGTC CTATTGTGGC CCCTGGAGAA
    AATATGACTT TTCAATGCCA AGGGGAACTA CCAGACTCAA CATTTGTCCT GTTGAAGGAA
    GGCACTCAAC AGCCTTTGGA ACAACAGAGC CCAAGTGGGT ACAGGGCTGA CTTCTGGATG
    CCAGCAGTGA GAGGTGATGA TTCTGGGGTC TATAGCTGTG TTTATTATTT GGACTCTGCT
    CCCTTTGCGG CTTCTAATCA CAGTGACTTC CTGGAGATCT GGGTGACTGA TAAGCCCCCT
    AAGCCCTCGC TGTCAGCCTG GCCCAGCACC ATGTTCAAGC TAGGCAAGGA CATCACCCTT
    CAGTGCCGTG GCCCCCTGCC AGGTGTTGAA TTTGTCCTGG AACATGATGG AGAAGAAGCA
    CCTCAGCAGT TCTCAGAGGA TGGGGACTTT GTCATCAACA ATGTAGAAGG AAAAGGCATT
    GGCAACTACA GCTGCAGCTA CCGCCTCCAG GCCTATCCTG ATATCTGGTC AGAGCCTAGT
    GATGCCCTGG AACTGGTGGG GGCAGCAGGG CCTGCTGCCC AGGAGTGCAC TGTGGGGAAC
    ATTGTCCGAA GTAGTCTGAT TGTGGTGGTA GTTGTAGCTT TGGGGGTAGT GCTAGCCATA
    GAATGGAAGA AGTGGCCTCG ACTCCGAACC AGGGACTCAG AGACAGATGG AAGAGACCAG
    ACCATAGCCC TTGAAGAGTG TAACCAAGAA GGAGAACCAG CCACCAACAC CAGCTCTCCC
    TCCTCCGTCT CTCAGGGAAC CTCAGTGGAA CTGCCAGTCC CAGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45777
    Clone ID Related Accession (Same CDS sequence) XM_014111976.1 , XM_014111977.1 , XM_014111975.1
    Accession Version XM_014111976.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GTGAGTACAA GGAAGGGCTG AGTCTGGGTA TGACATCAAT AGTGATGCAA
    TCTCAACCGG AGCTGTGGAT AGAGACCAAT TACCCCCAGA CCCCTTGGGA GAACATCACA
    CTTTGGTGCA AAAGCCCCTC TCGGATTTCA AGCAAGTTTC TGCTGCTGAA GGATAAGACA
    CAGATGACCT GGATCCGCCC TTCCTCCAAG ACCTTCCAAG TTTCATTCCC TATAGGTGCC
    CTTACTAAGT CCAATGCAGG TCTTTACAGG TGCTGCTACT GGAAGGAAAC AGGCTGGTCA
    GAGCCCAGTA AAGTTTTAGA GTTGGAGGCA CCAGGCCAGC TGCCCAAGCC CATCTTCTGG
    ATCCAGGCTG AGACCTACCC TCTTACTGGA TGTAATGTTA ACATACTCTG CCATGGCTGG
    CTGCAGGATT TGGTATTCAT GCTGTTCAAA GAGGGATATG CAGAGCCTGT GGATTACCAA
    ATCCCAACTG GGACAGTGGC CATATTCTCC ATCGCCAACA TGACACCTGA GAGTGAAGGG
    GTTTACATCT GCCGCACTCA TATCCAGATG CTCCCCACTC TGTGGTCAGA GCCCAGCAAC
    CCCCTGAAGC TGATTGTGGC AGGACTCTAT CCCAAACCAA CTCTGACAGC ATATCCTGGG
    CCCATTATGG CACCTGGAGA AAGCCTGAAC CTCAGGTGTC AGGCACCAAT CTACGGAATG
    ACCTTTGCTC TAATAAGGCT TGAAGATTTG AAGAAATCCT TTTACCGCAA AAGGCCAATA
    AAAAATGAGG CATATTTCTT CTTCCGGGCT TTGAAAATCC ATGATGCTGG ACATTACCTC
    TGTTTTTACT ATGATGAGTC ATACAGGGGT TCACTCCTTA GTGATATCCT GAAAATCTGG
    GTGACCGACA CTTTCCCCAA GACCTGGCTA CTTGCTCAGC CCAGTCCTGT GGTCCAGATG
    GGTCAGAACG TGAGCCTGTG GTGTCGAGGG CCAGTGGATG GAGTGGGGCT TGCACTCTAT
    AAGAAAGGAG AAGACAAACC ACTTCAGCTT TTGGATACCA CCAGCATTGA TGACGAGTCA
    TTCTTCCTCA ACAATGTGAC CTACAGTGAT GCTGGCATCT ATAGTTGCCA CTATCTCCTC
    TCCTGGAAGA CCTCCATCAG GATGACATCA CACAACACTG TGGAACTTGT GGTTGTAGAT
    AAGCCCCCCA AACCCTCCCT TTCAGCCTGG CCCAGCACCA TGTTCAAGCT AAGAAAGGCC
    ATTACCCTTC AGCACCGAGT ACCCCATCCA GTACTTGAAT TTTCTCTGGA ATGGGAAGAA
    AGAGCAACAT TCCAAAAATT CTTGGTAGAT GGAGATTTCA TCATCAGTAA TGCTGAAGGA
    AAAGGCACAG AAACTTACAG TTGCAGCTAT CGCATAGAGG CACACCCCAA CATCTGGTCA
    GATCGCAGTG AGCCTCTGAA GCTGATGGGG CCAGCAGGCT TTCTTACCTG GAATTACATT
    CTGAATGAAG CTATCAGATT GTCCCTAATC ATGCAGCTTG TTGCCTTGCT GTTGGTAGTG
    CTGTGGATAA GGTGGAAGTG TCGGAGACTC AGAATCAGAG AAGCCTGGTT GCTGGGAACA
    GCTCAAGGGG TCACCATGCT CTTCATAGTC ACGGCCCTTC TCTGCTGTGG ACTGTGCAAT
    GGGGTATTGA CAGAAGAGAC TGAAATAATC ATGCCAACCC CTAAGCCTGA GCTGTGGGCA
    GAGACCAACT TCCCTCTTGC CCCGTGGAAG AACTTAACCC TCTGGTGCAG AAGCCCTTCT
    GGCTCAACTA AGGAGTTTGT GTTGCTGAAG GACGGGACTG GGTGGATTGC AACTCGCCCG
    GCCTCCGAGC AGGTCCGGGC TGCCTTTCCC CTTGGCGCCC TAACCCAGAG CCATACCGGG
    AGTTACCACT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCCAGTGA GGCACTTGAG
    CTGGTGGGGA CAGACATCCT CCCCAAACCT GTCATTTCTG CTTCCCCCCC AATTCGGGGC
    CAGAAACTGC AAATCCGGTG CAAAGGATGG CTGGCAGGCA TGGAGTTCGT TCTGTATAAG
    GAGGGAGTGC AGGAACCTGT CCAGCAACTT CATGCCGTTG GGAGAGAAGC CTTCTTTACA
    ATCCAAAGAA TGGAGGATAA AGACGAAGGC AATTATAGCT GCCGTACTCA TACTGAAAAG
    CGCCCCTTCA AGTGGTCTGA GCCCAGTGAT CCCATGGAGC TTGTCATAAA AGAAATGTAC
    CCCAAGCCCT TTTTCAAGAC ATGGGCCAGC CCTGTGGTCA CTCCTGGTGC CCGAGTGACT
    TTCAATTGCT CCACTCCCCA GCAGCACATG AGCTTTATTC TTTACAAAGA TGGAAGTGAA
    ATAGCATCCA GTGACAGGTC TTGGGCAAGT CCAGGGGCCA GTGCGGCTCA CTTTCTGATC
    ATTTCAGTGG GCACTGGTGA TGGAGGGAAT TATAGCTGCC GCTATTATGA CTTCGCTATC
    TGGTCTGAGC CCAGCGACCC TGTGGAGCTC GTGGTGACAG AATTCTACCC CAAACCTACT
    CTTCTGGCAC ACCCGGGTCC TGTGGTGCTT CCTGGAAAGA ATGTGACCCT GCGCTGCCAA
    GGGGCTTTCC AAGGCATGAG ATTTGCCCTC TTGCAGGAGG GAACCCAGGT TCCCTTACAG
    TTCCAGAGCA CCTCCGGGAA TTCAGCTGAC TTCCTCCTCC ACACTGTTAG AGCAGAGGAC
    TCTGGGAACT ACAGTTGTGT TTACTACGAG ACAACCATGT CAAATAGGGG ATCACATCTC
    AGCAAGCCCA TTATGATCTG GGTGACTGAC ACATTTCCCA AGCCATGGTT GTTTGCTGAG
    CCCAGTTCTG TGGTTCCTAT GGGGCAGAAT GTTACTCTCT GGTGCCAAGG GCCAGTCCAT
    GGAGTAGGAT ACATTCTGCA CAAAGAAAGA GAAACCACTT CAGTGCAGCT CTGGGGATCC
    ACCAGTAATG ATGGGGCATT CCCTATCACC AATATATCTG GTGCTAACAT AGGGCGTTAC
    AGCTGCTGCT ACCACCCTGA CTGGACCAGC CCTATCAAGA TACAGCCTAG CAACACTCTG
    GAACTCATAG TCACAGGTTT GCTCCCCAAA CCCAGCCTCT TAGCCCAGCC AGGTCCTATT
    GTGGCCCCTG GAGAAAATAT GACTTTTCAA TGCCAAGGGG AACTACCAGA CTCAACATTT
    GTCCTGTTGA AGGAAGGCAC TCAACAGCCT TTGGAACAAC AGAGCCCAAG TGGGTACAGG
    GCTGACTTCT GGATGCCAGC AGTGAGAGGT GATGATTCTG GGGTCTATAG CTGTGTTTAT
    TATTTGGACT CTGCTCCCTT TGCGGCTTCT AATCACAGTG ACTTCCTGGA GATCTGGGTG
    ACTGATAAGC CCCCTAAGCC CTCGCTGTCA GCCTGGCCCA GCACCATGTT CAAGCTAGGC
    AAGGACATCA CCCTTCAGTG CCGTGGCCCC CTGCCAGGTG TTGAATTTGT CCTGGAACAT
    GATGGAGAAG AAGCACCTCA GCAGTTCTCA GAGGATGGGG ACTTTGTCAT CAACAATGTA
    GAAGGAAAAG GCATTGGCAA CTACAGCTGC AGCTACCGCC TCCAGGCCTA TCCTGATATC
    TGGTCAGAGC CTAGTGATGC CCTGGAACTG GTGGGGGCAG CAGGGCCTGC TGCCCAGGAG
    TGCACTGTGG GGAACATTGT CCGAAGTAGT CTGATTGTGG TGGTAGTTGT AGCTTTGGGG
    GTAGTGCTAG CCATAGAATG GAAGAAGTGG CCTCGACTCC GAACCAGGGA CTCAGAGACA
    GATGGAAGAG ACCAGACCAT AGCCCTTGAA GAGTGTAACC AAGAAGGAGA ACCAGCCACC
    AACACCAGCT CTCCCTCCTC CGTCTCTCAG GGAACCTCAG TGGAACTGCC AGTCCCAGTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967451.1
    CDS248..4270
    Translation

    Target ORF information:

    RefSeq Version XM_014111976.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111976.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GTGAGTACAA GGAAGGGCTG AGTCTGGGTA TGACATCAAT AGTGATGCAA
    TCTCAACCGG AGCTGTGGAT AGAGACCAAT TACCCCCAGA CCCCTTGGGA GAACATCACA
    CTTTGGTGCA AAAGCCCCTC TCGGATTTCA AGCAAGTTTC TGCTGCTGAA GGATAAGACA
    CAGATGACCT GGATCCGCCC TTCCTCCAAG ACCTTCCAAG TTTCATTCCC TATAGGTGCC
    CTTACTAAGT CCAATGCAGG TCTTTACAGG TGCTGCTACT GGAAGGAAAC AGGCTGGTCA
    GAGCCCAGTA AAGTTTTAGA GTTGGAGGCA CCAGGCCAGC TGCCCAAGCC CATCTTCTGG
    ATCCAGGCTG AGACCTACCC TCTTACTGGA TGTAATGTTA ACATACTCTG CCATGGCTGG
    CTGCAGGATT TGGTATTCAT GCTGTTCAAA GAGGGATATG CAGAGCCTGT GGATTACCAA
    ATCCCAACTG GGACAGTGGC CATATTCTCC ATCGCCAACA TGACACCTGA GAGTGAAGGG
    GTTTACATCT GCCGCACTCA TATCCAGATG CTCCCCACTC TGTGGTCAGA GCCCAGCAAC
    CCCCTGAAGC TGATTGTGGC AGGACTCTAT CCCAAACCAA CTCTGACAGC ATATCCTGGG
    CCCATTATGG CACCTGGAGA AAGCCTGAAC CTCAGGTGTC AGGCACCAAT CTACGGAATG
    ACCTTTGCTC TAATAAGGCT TGAAGATTTG AAGAAATCCT TTTACCGCAA AAGGCCAATA
    AAAAATGAGG CATATTTCTT CTTCCGGGCT TTGAAAATCC ATGATGCTGG ACATTACCTC
    TGTTTTTACT ATGATGAGTC ATACAGGGGT TCACTCCTTA GTGATATCCT GAAAATCTGG
    GTGACCGACA CTTTCCCCAA GACCTGGCTA CTTGCTCAGC CCAGTCCTGT GGTCCAGATG
    GGTCAGAACG TGAGCCTGTG GTGTCGAGGG CCAGTGGATG GAGTGGGGCT TGCACTCTAT
    AAGAAAGGAG AAGACAAACC ACTTCAGCTT TTGGATACCA CCAGCATTGA TGACGAGTCA
    TTCTTCCTCA ACAATGTGAC CTACAGTGAT GCTGGCATCT ATAGTTGCCA CTATCTCCTC
    TCCTGGAAGA CCTCCATCAG GATGACATCA CACAACACTG TGGAACTTGT GGTTGTAGAT
    AAGCCCCCCA AACCCTCCCT TTCAGCCTGG CCCAGCACCA TGTTCAAGCT AAGAAAGGCC
    ATTACCCTTC AGCACCGAGT ACCCCATCCA GTACTTGAAT TTTCTCTGGA ATGGGAAGAA
    AGAGCAACAT TCCAAAAATT CTTGGTAGAT GGAGATTTCA TCATCAGTAA TGCTGAAGGA
    AAAGGCACAG AAACTTACAG TTGCAGCTAT CGCATAGAGG CACACCCCAA CATCTGGTCA
    GATCGCAGTG AGCCTCTGAA GCTGATGGGG CCAGCAGGCT TTCTTACCTG GAATTACATT
    CTGAATGAAG CTATCAGATT GTCCCTAATC ATGCAGCTTG TTGCCTTGCT GTTGGTAGTG
    CTGTGGATAA GGTGGAAGTG TCGGAGACTC AGAATCAGAG AAGCCTGGTT GCTGGGAACA
    GCTCAAGGGG TCACCATGCT CTTCATAGTC ACGGCCCTTC TCTGCTGTGG ACTGTGCAAT
    GGGGTATTGA CAGAAGAGAC TGAAATAATC ATGCCAACCC CTAAGCCTGA GCTGTGGGCA
    GAGACCAACT TCCCTCTTGC CCCGTGGAAG AACTTAACCC TCTGGTGCAG AAGCCCTTCT
    GGCTCAACTA AGGAGTTTGT GTTGCTGAAG GACGGGACTG GGTGGATTGC AACTCGCCCG
    GCCTCCGAGC AGGTCCGGGC TGCCTTTCCC CTTGGCGCCC TAACCCAGAG CCATACCGGG
    AGTTACCACT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCCAGTGA GGCACTTGAG
    CTGGTGGGGA CAGACATCCT CCCCAAACCT GTCATTTCTG CTTCCCCCCC AATTCGGGGC
    CAGAAACTGC AAATCCGGTG CAAAGGATGG CTGGCAGGCA TGGAGTTCGT TCTGTATAAG
    GAGGGAGTGC AGGAACCTGT CCAGCAACTT CATGCCGTTG GGAGAGAAGC CTTCTTTACA
    ATCCAAAGAA TGGAGGATAA AGACGAAGGC AATTATAGCT GCCGTACTCA TACTGAAAAG
    CGCCCCTTCA AGTGGTCTGA GCCCAGTGAT CCCATGGAGC TTGTCATAAA AGAAATGTAC
    CCCAAGCCCT TTTTCAAGAC ATGGGCCAGC CCTGTGGTCA CTCCTGGTGC CCGAGTGACT
    TTCAATTGCT CCACTCCCCA GCAGCACATG AGCTTTATTC TTTACAAAGA TGGAAGTGAA
    ATAGCATCCA GTGACAGGTC TTGGGCAAGT CCAGGGGCCA GTGCGGCTCA CTTTCTGATC
    ATTTCAGTGG GCACTGGTGA TGGAGGGAAT TATAGCTGCC GCTATTATGA CTTCGCTATC
    TGGTCTGAGC CCAGCGACCC TGTGGAGCTC GTGGTGACAG AATTCTACCC CAAACCTACT
    CTTCTGGCAC ACCCGGGTCC TGTGGTGCTT CCTGGAAAGA ATGTGACCCT GCGCTGCCAA
    GGGGCTTTCC AAGGCATGAG ATTTGCCCTC TTGCAGGAGG GAACCCAGGT TCCCTTACAG
    TTCCAGAGCA CCTCCGGGAA TTCAGCTGAC TTCCTCCTCC ACACTGTTAG AGCAGAGGAC
    TCTGGGAACT ACAGTTGTGT TTACTACGAG ACAACCATGT CAAATAGGGG ATCACATCTC
    AGCAAGCCCA TTATGATCTG GGTGACTGAC ACATTTCCCA AGCCATGGTT GTTTGCTGAG
    CCCAGTTCTG TGGTTCCTAT GGGGCAGAAT GTTACTCTCT GGTGCCAAGG GCCAGTCCAT
    GGAGTAGGAT ACATTCTGCA CAAAGAAAGA GAAACCACTT CAGTGCAGCT CTGGGGATCC
    ACCAGTAATG ATGGGGCATT CCCTATCACC AATATATCTG GTGCTAACAT AGGGCGTTAC
    AGCTGCTGCT ACCACCCTGA CTGGACCAGC CCTATCAAGA TACAGCCTAG CAACACTCTG
    GAACTCATAG TCACAGGTTT GCTCCCCAAA CCCAGCCTCT TAGCCCAGCC AGGTCCTATT
    GTGGCCCCTG GAGAAAATAT GACTTTTCAA TGCCAAGGGG AACTACCAGA CTCAACATTT
    GTCCTGTTGA AGGAAGGCAC TCAACAGCCT TTGGAACAAC AGAGCCCAAG TGGGTACAGG
    GCTGACTTCT GGATGCCAGC AGTGAGAGGT GATGATTCTG GGGTCTATAG CTGTGTTTAT
    TATTTGGACT CTGCTCCCTT TGCGGCTTCT AATCACAGTG ACTTCCTGGA GATCTGGGTG
    ACTGATAAGC CCCCTAAGCC CTCGCTGTCA GCCTGGCCCA GCACCATGTT CAAGCTAGGC
    AAGGACATCA CCCTTCAGTG CCGTGGCCCC CTGCCAGGTG TTGAATTTGT CCTGGAACAT
    GATGGAGAAG AAGCACCTCA GCAGTTCTCA GAGGATGGGG ACTTTGTCAT CAACAATGTA
    GAAGGAAAAG GCATTGGCAA CTACAGCTGC AGCTACCGCC TCCAGGCCTA TCCTGATATC
    TGGTCAGAGC CTAGTGATGC CCTGGAACTG GTGGGGGCAG CAGGGCCTGC TGCCCAGGAG
    TGCACTGTGG GGAACATTGT CCGAAGTAGT CTGATTGTGG TGGTAGTTGT AGCTTTGGGG
    GTAGTGCTAG CCATAGAATG GAAGAAGTGG CCTCGACTCC GAACCAGGGA CTCAGAGACA
    GATGGAAGAG ACCAGACCAT AGCCCTTGAA GAGTGTAACC AAGAAGGAGA ACCAGCCACC
    AACACCAGCT CTCCCTCCTC CGTCTCTCAG GGAACCTCAG TGGAACTGCC AGTCCCAGTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45777
    Clone ID Related Accession (Same CDS sequence) XM_014111976.1 , XM_014111977.1 , XM_014111975.1
    Accession Version XM_014111977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GTGAGTACAA GGAAGGGCTG AGTCTGGGTA TGACATCAAT AGTGATGCAA
    TCTCAACCGG AGCTGTGGAT AGAGACCAAT TACCCCCAGA CCCCTTGGGA GAACATCACA
    CTTTGGTGCA AAAGCCCCTC TCGGATTTCA AGCAAGTTTC TGCTGCTGAA GGATAAGACA
    CAGATGACCT GGATCCGCCC TTCCTCCAAG ACCTTCCAAG TTTCATTCCC TATAGGTGCC
    CTTACTAAGT CCAATGCAGG TCTTTACAGG TGCTGCTACT GGAAGGAAAC AGGCTGGTCA
    GAGCCCAGTA AAGTTTTAGA GTTGGAGGCA CCAGGCCAGC TGCCCAAGCC CATCTTCTGG
    ATCCAGGCTG AGACCTACCC TCTTACTGGA TGTAATGTTA ACATACTCTG CCATGGCTGG
    CTGCAGGATT TGGTATTCAT GCTGTTCAAA GAGGGATATG CAGAGCCTGT GGATTACCAA
    ATCCCAACTG GGACAGTGGC CATATTCTCC ATCGCCAACA TGACACCTGA GAGTGAAGGG
    GTTTACATCT GCCGCACTCA TATCCAGATG CTCCCCACTC TGTGGTCAGA GCCCAGCAAC
    CCCCTGAAGC TGATTGTGGC AGGACTCTAT CCCAAACCAA CTCTGACAGC ATATCCTGGG
    CCCATTATGG CACCTGGAGA AAGCCTGAAC CTCAGGTGTC AGGCACCAAT CTACGGAATG
    ACCTTTGCTC TAATAAGGCT TGAAGATTTG AAGAAATCCT TTTACCGCAA AAGGCCAATA
    AAAAATGAGG CATATTTCTT CTTCCGGGCT TTGAAAATCC ATGATGCTGG ACATTACCTC
    TGTTTTTACT ATGATGAGTC ATACAGGGGT TCACTCCTTA GTGATATCCT GAAAATCTGG
    GTGACCGACA CTTTCCCCAA GACCTGGCTA CTTGCTCAGC CCAGTCCTGT GGTCCAGATG
    GGTCAGAACG TGAGCCTGTG GTGTCGAGGG CCAGTGGATG GAGTGGGGCT TGCACTCTAT
    AAGAAAGGAG AAGACAAACC ACTTCAGCTT TTGGATACCA CCAGCATTGA TGACGAGTCA
    TTCTTCCTCA ACAATGTGAC CTACAGTGAT GCTGGCATCT ATAGTTGCCA CTATCTCCTC
    TCCTGGAAGA CCTCCATCAG GATGACATCA CACAACACTG TGGAACTTGT GGTTGTAGAT
    AAGCCCCCCA AACCCTCCCT TTCAGCCTGG CCCAGCACCA TGTTCAAGCT AAGAAAGGCC
    ATTACCCTTC AGCACCGAGT ACCCCATCCA GTACTTGAAT TTTCTCTGGA ATGGGAAGAA
    AGAGCAACAT TCCAAAAATT CTTGGTAGAT GGAGATTTCA TCATCAGTAA TGCTGAAGGA
    AAAGGCACAG AAACTTACAG TTGCAGCTAT CGCATAGAGG CACACCCCAA CATCTGGTCA
    GATCGCAGTG AGCCTCTGAA GCTGATGGGG CCAGCAGGCT TTCTTACCTG GAATTACATT
    CTGAATGAAG CTATCAGATT GTCCCTAATC ATGCAGCTTG TTGCCTTGCT GTTGGTAGTG
    CTGTGGATAA GGTGGAAGTG TCGGAGACTC AGAATCAGAG AAGCCTGGTT GCTGGGAACA
    GCTCAAGGGG TCACCATGCT CTTCATAGTC ACGGCCCTTC TCTGCTGTGG ACTGTGCAAT
    GGGGTATTGA CAGAAGAGAC TGAAATAATC ATGCCAACCC CTAAGCCTGA GCTGTGGGCA
    GAGACCAACT TCCCTCTTGC CCCGTGGAAG AACTTAACCC TCTGGTGCAG AAGCCCTTCT
    GGCTCAACTA AGGAGTTTGT GTTGCTGAAG GACGGGACTG GGTGGATTGC AACTCGCCCG
    GCCTCCGAGC AGGTCCGGGC TGCCTTTCCC CTTGGCGCCC TAACCCAGAG CCATACCGGG
    AGTTACCACT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCCAGTGA GGCACTTGAG
    CTGGTGGGGA CAGACATCCT CCCCAAACCT GTCATTTCTG CTTCCCCCCC AATTCGGGGC
    CAGAAACTGC AAATCCGGTG CAAAGGATGG CTGGCAGGCA TGGAGTTCGT TCTGTATAAG
    GAGGGAGTGC AGGAACCTGT CCAGCAACTT CATGCCGTTG GGAGAGAAGC CTTCTTTACA
    ATCCAAAGAA TGGAGGATAA AGACGAAGGC AATTATAGCT GCCGTACTCA TACTGAAAAG
    CGCCCCTTCA AGTGGTCTGA GCCCAGTGAT CCCATGGAGC TTGTCATAAA AGAAATGTAC
    CCCAAGCCCT TTTTCAAGAC ATGGGCCAGC CCTGTGGTCA CTCCTGGTGC CCGAGTGACT
    TTCAATTGCT CCACTCCCCA GCAGCACATG AGCTTTATTC TTTACAAAGA TGGAAGTGAA
    ATAGCATCCA GTGACAGGTC TTGGGCAAGT CCAGGGGCCA GTGCGGCTCA CTTTCTGATC
    ATTTCAGTGG GCACTGGTGA TGGAGGGAAT TATAGCTGCC GCTATTATGA CTTCGCTATC
    TGGTCTGAGC CCAGCGACCC TGTGGAGCTC GTGGTGACAG AATTCTACCC CAAACCTACT
    CTTCTGGCAC ACCCGGGTCC TGTGGTGCTT CCTGGAAAGA ATGTGACCCT GCGCTGCCAA
    GGGGCTTTCC AAGGCATGAG ATTTGCCCTC TTGCAGGAGG GAACCCAGGT TCCCTTACAG
    TTCCAGAGCA CCTCCGGGAA TTCAGCTGAC TTCCTCCTCC ACACTGTTAG AGCAGAGGAC
    TCTGGGAACT ACAGTTGTGT TTACTACGAG ACAACCATGT CAAATAGGGG ATCACATCTC
    AGCAAGCCCA TTATGATCTG GGTGACTGAC ACATTTCCCA AGCCATGGTT GTTTGCTGAG
    CCCAGTTCTG TGGTTCCTAT GGGGCAGAAT GTTACTCTCT GGTGCCAAGG GCCAGTCCAT
    GGAGTAGGAT ACATTCTGCA CAAAGAAAGA GAAACCACTT CAGTGCAGCT CTGGGGATCC
    ACCAGTAATG ATGGGGCATT CCCTATCACC AATATATCTG GTGCTAACAT AGGGCGTTAC
    AGCTGCTGCT ACCACCCTGA CTGGACCAGC CCTATCAAGA TACAGCCTAG CAACACTCTG
    GAACTCATAG TCACAGGTTT GCTCCCCAAA CCCAGCCTCT TAGCCCAGCC AGGTCCTATT
    GTGGCCCCTG GAGAAAATAT GACTTTTCAA TGCCAAGGGG AACTACCAGA CTCAACATTT
    GTCCTGTTGA AGGAAGGCAC TCAACAGCCT TTGGAACAAC AGAGCCCAAG TGGGTACAGG
    GCTGACTTCT GGATGCCAGC AGTGAGAGGT GATGATTCTG GGGTCTATAG CTGTGTTTAT
    TATTTGGACT CTGCTCCCTT TGCGGCTTCT AATCACAGTG ACTTCCTGGA GATCTGGGTG
    ACTGATAAGC CCCCTAAGCC CTCGCTGTCA GCCTGGCCCA GCACCATGTT CAAGCTAGGC
    AAGGACATCA CCCTTCAGTG CCGTGGCCCC CTGCCAGGTG TTGAATTTGT CCTGGAACAT
    GATGGAGAAG AAGCACCTCA GCAGTTCTCA GAGGATGGGG ACTTTGTCAT CAACAATGTA
    GAAGGAAAAG GCATTGGCAA CTACAGCTGC AGCTACCGCC TCCAGGCCTA TCCTGATATC
    TGGTCAGAGC CTAGTGATGC CCTGGAACTG GTGGGGGCAG CAGGGCCTGC TGCCCAGGAG
    TGCACTGTGG GGAACATTGT CCGAAGTAGT CTGATTGTGG TGGTAGTTGT AGCTTTGGGG
    GTAGTGCTAG CCATAGAATG GAAGAAGTGG CCTCGACTCC GAACCAGGGA CTCAGAGACA
    GATGGAAGAG ACCAGACCAT AGCCCTTGAA GAGTGTAACC AAGAAGGAGA ACCAGCCACC
    AACACCAGCT CTCCCTCCTC CGTCTCTCAG GGAACCTCAG TGGAACTGCC AGTCCCAGTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967452.1
    CDS182..4204
    Translation

    Target ORF information:

    RefSeq Version XM_014111977.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GTGAGTACAA GGAAGGGCTG AGTCTGGGTA TGACATCAAT AGTGATGCAA
    TCTCAACCGG AGCTGTGGAT AGAGACCAAT TACCCCCAGA CCCCTTGGGA GAACATCACA
    CTTTGGTGCA AAAGCCCCTC TCGGATTTCA AGCAAGTTTC TGCTGCTGAA GGATAAGACA
    CAGATGACCT GGATCCGCCC TTCCTCCAAG ACCTTCCAAG TTTCATTCCC TATAGGTGCC
    CTTACTAAGT CCAATGCAGG TCTTTACAGG TGCTGCTACT GGAAGGAAAC AGGCTGGTCA
    GAGCCCAGTA AAGTTTTAGA GTTGGAGGCA CCAGGCCAGC TGCCCAAGCC CATCTTCTGG
    ATCCAGGCTG AGACCTACCC TCTTACTGGA TGTAATGTTA ACATACTCTG CCATGGCTGG
    CTGCAGGATT TGGTATTCAT GCTGTTCAAA GAGGGATATG CAGAGCCTGT GGATTACCAA
    ATCCCAACTG GGACAGTGGC CATATTCTCC ATCGCCAACA TGACACCTGA GAGTGAAGGG
    GTTTACATCT GCCGCACTCA TATCCAGATG CTCCCCACTC TGTGGTCAGA GCCCAGCAAC
    CCCCTGAAGC TGATTGTGGC AGGACTCTAT CCCAAACCAA CTCTGACAGC ATATCCTGGG
    CCCATTATGG CACCTGGAGA AAGCCTGAAC CTCAGGTGTC AGGCACCAAT CTACGGAATG
    ACCTTTGCTC TAATAAGGCT TGAAGATTTG AAGAAATCCT TTTACCGCAA AAGGCCAATA
    AAAAATGAGG CATATTTCTT CTTCCGGGCT TTGAAAATCC ATGATGCTGG ACATTACCTC
    TGTTTTTACT ATGATGAGTC ATACAGGGGT TCACTCCTTA GTGATATCCT GAAAATCTGG
    GTGACCGACA CTTTCCCCAA GACCTGGCTA CTTGCTCAGC CCAGTCCTGT GGTCCAGATG
    GGTCAGAACG TGAGCCTGTG GTGTCGAGGG CCAGTGGATG GAGTGGGGCT TGCACTCTAT
    AAGAAAGGAG AAGACAAACC ACTTCAGCTT TTGGATACCA CCAGCATTGA TGACGAGTCA
    TTCTTCCTCA ACAATGTGAC CTACAGTGAT GCTGGCATCT ATAGTTGCCA CTATCTCCTC
    TCCTGGAAGA CCTCCATCAG GATGACATCA CACAACACTG TGGAACTTGT GGTTGTAGAT
    AAGCCCCCCA AACCCTCCCT TTCAGCCTGG CCCAGCACCA TGTTCAAGCT AAGAAAGGCC
    ATTACCCTTC AGCACCGAGT ACCCCATCCA GTACTTGAAT TTTCTCTGGA ATGGGAAGAA
    AGAGCAACAT TCCAAAAATT CTTGGTAGAT GGAGATTTCA TCATCAGTAA TGCTGAAGGA
    AAAGGCACAG AAACTTACAG TTGCAGCTAT CGCATAGAGG CACACCCCAA CATCTGGTCA
    GATCGCAGTG AGCCTCTGAA GCTGATGGGG CCAGCAGGCT TTCTTACCTG GAATTACATT
    CTGAATGAAG CTATCAGATT GTCCCTAATC ATGCAGCTTG TTGCCTTGCT GTTGGTAGTG
    CTGTGGATAA GGTGGAAGTG TCGGAGACTC AGAATCAGAG AAGCCTGGTT GCTGGGAACA
    GCTCAAGGGG TCACCATGCT CTTCATAGTC ACGGCCCTTC TCTGCTGTGG ACTGTGCAAT
    GGGGTATTGA CAGAAGAGAC TGAAATAATC ATGCCAACCC CTAAGCCTGA GCTGTGGGCA
    GAGACCAACT TCCCTCTTGC CCCGTGGAAG AACTTAACCC TCTGGTGCAG AAGCCCTTCT
    GGCTCAACTA AGGAGTTTGT GTTGCTGAAG GACGGGACTG GGTGGATTGC AACTCGCCCG
    GCCTCCGAGC AGGTCCGGGC TGCCTTTCCC CTTGGCGCCC TAACCCAGAG CCATACCGGG
    AGTTACCACT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCCAGTGA GGCACTTGAG
    CTGGTGGGGA CAGACATCCT CCCCAAACCT GTCATTTCTG CTTCCCCCCC AATTCGGGGC
    CAGAAACTGC AAATCCGGTG CAAAGGATGG CTGGCAGGCA TGGAGTTCGT TCTGTATAAG
    GAGGGAGTGC AGGAACCTGT CCAGCAACTT CATGCCGTTG GGAGAGAAGC CTTCTTTACA
    ATCCAAAGAA TGGAGGATAA AGACGAAGGC AATTATAGCT GCCGTACTCA TACTGAAAAG
    CGCCCCTTCA AGTGGTCTGA GCCCAGTGAT CCCATGGAGC TTGTCATAAA AGAAATGTAC
    CCCAAGCCCT TTTTCAAGAC ATGGGCCAGC CCTGTGGTCA CTCCTGGTGC CCGAGTGACT
    TTCAATTGCT CCACTCCCCA GCAGCACATG AGCTTTATTC TTTACAAAGA TGGAAGTGAA
    ATAGCATCCA GTGACAGGTC TTGGGCAAGT CCAGGGGCCA GTGCGGCTCA CTTTCTGATC
    ATTTCAGTGG GCACTGGTGA TGGAGGGAAT TATAGCTGCC GCTATTATGA CTTCGCTATC
    TGGTCTGAGC CCAGCGACCC TGTGGAGCTC GTGGTGACAG AATTCTACCC CAAACCTACT
    CTTCTGGCAC ACCCGGGTCC TGTGGTGCTT CCTGGAAAGA ATGTGACCCT GCGCTGCCAA
    GGGGCTTTCC AAGGCATGAG ATTTGCCCTC TTGCAGGAGG GAACCCAGGT TCCCTTACAG
    TTCCAGAGCA CCTCCGGGAA TTCAGCTGAC TTCCTCCTCC ACACTGTTAG AGCAGAGGAC
    TCTGGGAACT ACAGTTGTGT TTACTACGAG ACAACCATGT CAAATAGGGG ATCACATCTC
    AGCAAGCCCA TTATGATCTG GGTGACTGAC ACATTTCCCA AGCCATGGTT GTTTGCTGAG
    CCCAGTTCTG TGGTTCCTAT GGGGCAGAAT GTTACTCTCT GGTGCCAAGG GCCAGTCCAT
    GGAGTAGGAT ACATTCTGCA CAAAGAAAGA GAAACCACTT CAGTGCAGCT CTGGGGATCC
    ACCAGTAATG ATGGGGCATT CCCTATCACC AATATATCTG GTGCTAACAT AGGGCGTTAC
    AGCTGCTGCT ACCACCCTGA CTGGACCAGC CCTATCAAGA TACAGCCTAG CAACACTCTG
    GAACTCATAG TCACAGGTTT GCTCCCCAAA CCCAGCCTCT TAGCCCAGCC AGGTCCTATT
    GTGGCCCCTG GAGAAAATAT GACTTTTCAA TGCCAAGGGG AACTACCAGA CTCAACATTT
    GTCCTGTTGA AGGAAGGCAC TCAACAGCCT TTGGAACAAC AGAGCCCAAG TGGGTACAGG
    GCTGACTTCT GGATGCCAGC AGTGAGAGGT GATGATTCTG GGGTCTATAG CTGTGTTTAT
    TATTTGGACT CTGCTCCCTT TGCGGCTTCT AATCACAGTG ACTTCCTGGA GATCTGGGTG
    ACTGATAAGC CCCCTAAGCC CTCGCTGTCA GCCTGGCCCA GCACCATGTT CAAGCTAGGC
    AAGGACATCA CCCTTCAGTG CCGTGGCCCC CTGCCAGGTG TTGAATTTGT CCTGGAACAT
    GATGGAGAAG AAGCACCTCA GCAGTTCTCA GAGGATGGGG ACTTTGTCAT CAACAATGTA
    GAAGGAAAAG GCATTGGCAA CTACAGCTGC AGCTACCGCC TCCAGGCCTA TCCTGATATC
    TGGTCAGAGC CTAGTGATGC CCTGGAACTG GTGGGGGCAG CAGGGCCTGC TGCCCAGGAG
    TGCACTGTGG GGAACATTGT CCGAAGTAGT CTGATTGTGG TGGTAGTTGT AGCTTTGGGG
    GTAGTGCTAG CCATAGAATG GAAGAAGTGG CCTCGACTCC GAACCAGGGA CTCAGAGACA
    GATGGAAGAG ACCAGACCAT AGCCCTTGAA GAGTGTAACC AAGAAGGAGA ACCAGCCACC
    AACACCAGCT CTCCCTCCTC CGTCTCTCAG GGAACCTCAG TGGAACTGCC AGTCCCAGTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45777
    Clone ID Related Accession (Same CDS sequence) XM_014111976.1 , XM_014111977.1 , XM_014111975.1
    Accession Version XM_014111975.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GTGAGTACAA GGAAGGGCTG AGTCTGGGTA TGACATCAAT AGTGATGCAA
    TCTCAACCGG AGCTGTGGAT AGAGACCAAT TACCCCCAGA CCCCTTGGGA GAACATCACA
    CTTTGGTGCA AAAGCCCCTC TCGGATTTCA AGCAAGTTTC TGCTGCTGAA GGATAAGACA
    CAGATGACCT GGATCCGCCC TTCCTCCAAG ACCTTCCAAG TTTCATTCCC TATAGGTGCC
    CTTACTAAGT CCAATGCAGG TCTTTACAGG TGCTGCTACT GGAAGGAAAC AGGCTGGTCA
    GAGCCCAGTA AAGTTTTAGA GTTGGAGGCA CCAGGCCAGC TGCCCAAGCC CATCTTCTGG
    ATCCAGGCTG AGACCTACCC TCTTACTGGA TGTAATGTTA ACATACTCTG CCATGGCTGG
    CTGCAGGATT TGGTATTCAT GCTGTTCAAA GAGGGATATG CAGAGCCTGT GGATTACCAA
    ATCCCAACTG GGACAGTGGC CATATTCTCC ATCGCCAACA TGACACCTGA GAGTGAAGGG
    GTTTACATCT GCCGCACTCA TATCCAGATG CTCCCCACTC TGTGGTCAGA GCCCAGCAAC
    CCCCTGAAGC TGATTGTGGC AGGACTCTAT CCCAAACCAA CTCTGACAGC ATATCCTGGG
    CCCATTATGG CACCTGGAGA AAGCCTGAAC CTCAGGTGTC AGGCACCAAT CTACGGAATG
    ACCTTTGCTC TAATAAGGCT TGAAGATTTG AAGAAATCCT TTTACCGCAA AAGGCCAATA
    AAAAATGAGG CATATTTCTT CTTCCGGGCT TTGAAAATCC ATGATGCTGG ACATTACCTC
    TGTTTTTACT ATGATGAGTC ATACAGGGGT TCACTCCTTA GTGATATCCT GAAAATCTGG
    GTGACCGACA CTTTCCCCAA GACCTGGCTA CTTGCTCAGC CCAGTCCTGT GGTCCAGATG
    GGTCAGAACG TGAGCCTGTG GTGTCGAGGG CCAGTGGATG GAGTGGGGCT TGCACTCTAT
    AAGAAAGGAG AAGACAAACC ACTTCAGCTT TTGGATACCA CCAGCATTGA TGACGAGTCA
    TTCTTCCTCA ACAATGTGAC CTACAGTGAT GCTGGCATCT ATAGTTGCCA CTATCTCCTC
    TCCTGGAAGA CCTCCATCAG GATGACATCA CACAACACTG TGGAACTTGT GGTTGTAGAT
    AAGCCCCCCA AACCCTCCCT TTCAGCCTGG CCCAGCACCA TGTTCAAGCT AAGAAAGGCC
    ATTACCCTTC AGCACCGAGT ACCCCATCCA GTACTTGAAT TTTCTCTGGA ATGGGAAGAA
    AGAGCAACAT TCCAAAAATT CTTGGTAGAT GGAGATTTCA TCATCAGTAA TGCTGAAGGA
    AAAGGCACAG AAACTTACAG TTGCAGCTAT CGCATAGAGG CACACCCCAA CATCTGGTCA
    GATCGCAGTG AGCCTCTGAA GCTGATGGGG CCAGCAGGCT TTCTTACCTG GAATTACATT
    CTGAATGAAG CTATCAGATT GTCCCTAATC ATGCAGCTTG TTGCCTTGCT GTTGGTAGTG
    CTGTGGATAA GGTGGAAGTG TCGGAGACTC AGAATCAGAG AAGCCTGGTT GCTGGGAACA
    GCTCAAGGGG TCACCATGCT CTTCATAGTC ACGGCCCTTC TCTGCTGTGG ACTGTGCAAT
    GGGGTATTGA CAGAAGAGAC TGAAATAATC ATGCCAACCC CTAAGCCTGA GCTGTGGGCA
    GAGACCAACT TCCCTCTTGC CCCGTGGAAG AACTTAACCC TCTGGTGCAG AAGCCCTTCT
    GGCTCAACTA AGGAGTTTGT GTTGCTGAAG GACGGGACTG GGTGGATTGC AACTCGCCCG
    GCCTCCGAGC AGGTCCGGGC TGCCTTTCCC CTTGGCGCCC TAACCCAGAG CCATACCGGG
    AGTTACCACT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCCAGTGA GGCACTTGAG
    CTGGTGGGGA CAGACATCCT CCCCAAACCT GTCATTTCTG CTTCCCCCCC AATTCGGGGC
    CAGAAACTGC AAATCCGGTG CAAAGGATGG CTGGCAGGCA TGGAGTTCGT TCTGTATAAG
    GAGGGAGTGC AGGAACCTGT CCAGCAACTT CATGCCGTTG GGAGAGAAGC CTTCTTTACA
    ATCCAAAGAA TGGAGGATAA AGACGAAGGC AATTATAGCT GCCGTACTCA TACTGAAAAG
    CGCCCCTTCA AGTGGTCTGA GCCCAGTGAT CCCATGGAGC TTGTCATAAA AGAAATGTAC
    CCCAAGCCCT TTTTCAAGAC ATGGGCCAGC CCTGTGGTCA CTCCTGGTGC CCGAGTGACT
    TTCAATTGCT CCACTCCCCA GCAGCACATG AGCTTTATTC TTTACAAAGA TGGAAGTGAA
    ATAGCATCCA GTGACAGGTC TTGGGCAAGT CCAGGGGCCA GTGCGGCTCA CTTTCTGATC
    ATTTCAGTGG GCACTGGTGA TGGAGGGAAT TATAGCTGCC GCTATTATGA CTTCGCTATC
    TGGTCTGAGC CCAGCGACCC TGTGGAGCTC GTGGTGACAG AATTCTACCC CAAACCTACT
    CTTCTGGCAC ACCCGGGTCC TGTGGTGCTT CCTGGAAAGA ATGTGACCCT GCGCTGCCAA
    GGGGCTTTCC AAGGCATGAG ATTTGCCCTC TTGCAGGAGG GAACCCAGGT TCCCTTACAG
    TTCCAGAGCA CCTCCGGGAA TTCAGCTGAC TTCCTCCTCC ACACTGTTAG AGCAGAGGAC
    TCTGGGAACT ACAGTTGTGT TTACTACGAG ACAACCATGT CAAATAGGGG ATCACATCTC
    AGCAAGCCCA TTATGATCTG GGTGACTGAC ACATTTCCCA AGCCATGGTT GTTTGCTGAG
    CCCAGTTCTG TGGTTCCTAT GGGGCAGAAT GTTACTCTCT GGTGCCAAGG GCCAGTCCAT
    GGAGTAGGAT ACATTCTGCA CAAAGAAAGA GAAACCACTT CAGTGCAGCT CTGGGGATCC
    ACCAGTAATG ATGGGGCATT CCCTATCACC AATATATCTG GTGCTAACAT AGGGCGTTAC
    AGCTGCTGCT ACCACCCTGA CTGGACCAGC CCTATCAAGA TACAGCCTAG CAACACTCTG
    GAACTCATAG TCACAGGTTT GCTCCCCAAA CCCAGCCTCT TAGCCCAGCC AGGTCCTATT
    GTGGCCCCTG GAGAAAATAT GACTTTTCAA TGCCAAGGGG AACTACCAGA CTCAACATTT
    GTCCTGTTGA AGGAAGGCAC TCAACAGCCT TTGGAACAAC AGAGCCCAAG TGGGTACAGG
    GCTGACTTCT GGATGCCAGC AGTGAGAGGT GATGATTCTG GGGTCTATAG CTGTGTTTAT
    TATTTGGACT CTGCTCCCTT TGCGGCTTCT AATCACAGTG ACTTCCTGGA GATCTGGGTG
    ACTGATAAGC CCCCTAAGCC CTCGCTGTCA GCCTGGCCCA GCACCATGTT CAAGCTAGGC
    AAGGACATCA CCCTTCAGTG CCGTGGCCCC CTGCCAGGTG TTGAATTTGT CCTGGAACAT
    GATGGAGAAG AAGCACCTCA GCAGTTCTCA GAGGATGGGG ACTTTGTCAT CAACAATGTA
    GAAGGAAAAG GCATTGGCAA CTACAGCTGC AGCTACCGCC TCCAGGCCTA TCCTGATATC
    TGGTCAGAGC CTAGTGATGC CCTGGAACTG GTGGGGGCAG CAGGGCCTGC TGCCCAGGAG
    TGCACTGTGG GGAACATTGT CCGAAGTAGT CTGATTGTGG TGGTAGTTGT AGCTTTGGGG
    GTAGTGCTAG CCATAGAATG GAAGAAGTGG CCTCGACTCC GAACCAGGGA CTCAGAGACA
    GATGGAAGAG ACCAGACCAT AGCCCTTGAA GAGTGTAACC AAGAAGGAGA ACCAGCCACC
    AACACCAGCT CTCCCTCCTC CGTCTCTCAG GGAACCTCAG TGGAACTGCC AGTCCCAGTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967450.1
    CDS244..4266
    Translation

    Target ORF information:

    RefSeq Version XM_014111975.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111975.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGA ACAGGCTGGG GGAGCGGGCT GCCATGCTGT GGACATTCAC AGTCTTGCTC 
    TTTTGCATTC GTGAGTACAA GGAAGGGCTG AGTCTGGGTA TGACATCAAT AGTGATGCAA
    TCTCAACCGG AGCTGTGGAT AGAGACCAAT TACCCCCAGA CCCCTTGGGA GAACATCACA
    CTTTGGTGCA AAAGCCCCTC TCGGATTTCA AGCAAGTTTC TGCTGCTGAA GGATAAGACA
    CAGATGACCT GGATCCGCCC TTCCTCCAAG ACCTTCCAAG TTTCATTCCC TATAGGTGCC
    CTTACTAAGT CCAATGCAGG TCTTTACAGG TGCTGCTACT GGAAGGAAAC AGGCTGGTCA
    GAGCCCAGTA AAGTTTTAGA GTTGGAGGCA CCAGGCCAGC TGCCCAAGCC CATCTTCTGG
    ATCCAGGCTG AGACCTACCC TCTTACTGGA TGTAATGTTA ACATACTCTG CCATGGCTGG
    CTGCAGGATT TGGTATTCAT GCTGTTCAAA GAGGGATATG CAGAGCCTGT GGATTACCAA
    ATCCCAACTG GGACAGTGGC CATATTCTCC ATCGCCAACA TGACACCTGA GAGTGAAGGG
    GTTTACATCT GCCGCACTCA TATCCAGATG CTCCCCACTC TGTGGTCAGA GCCCAGCAAC
    CCCCTGAAGC TGATTGTGGC AGGACTCTAT CCCAAACCAA CTCTGACAGC ATATCCTGGG
    CCCATTATGG CACCTGGAGA AAGCCTGAAC CTCAGGTGTC AGGCACCAAT CTACGGAATG
    ACCTTTGCTC TAATAAGGCT TGAAGATTTG AAGAAATCCT TTTACCGCAA AAGGCCAATA
    AAAAATGAGG CATATTTCTT CTTCCGGGCT TTGAAAATCC ATGATGCTGG ACATTACCTC
    TGTTTTTACT ATGATGAGTC ATACAGGGGT TCACTCCTTA GTGATATCCT GAAAATCTGG
    GTGACCGACA CTTTCCCCAA GACCTGGCTA CTTGCTCAGC CCAGTCCTGT GGTCCAGATG
    GGTCAGAACG TGAGCCTGTG GTGTCGAGGG CCAGTGGATG GAGTGGGGCT TGCACTCTAT
    AAGAAAGGAG AAGACAAACC ACTTCAGCTT TTGGATACCA CCAGCATTGA TGACGAGTCA
    TTCTTCCTCA ACAATGTGAC CTACAGTGAT GCTGGCATCT ATAGTTGCCA CTATCTCCTC
    TCCTGGAAGA CCTCCATCAG GATGACATCA CACAACACTG TGGAACTTGT GGTTGTAGAT
    AAGCCCCCCA AACCCTCCCT TTCAGCCTGG CCCAGCACCA TGTTCAAGCT AAGAAAGGCC
    ATTACCCTTC AGCACCGAGT ACCCCATCCA GTACTTGAAT TTTCTCTGGA ATGGGAAGAA
    AGAGCAACAT TCCAAAAATT CTTGGTAGAT GGAGATTTCA TCATCAGTAA TGCTGAAGGA
    AAAGGCACAG AAACTTACAG TTGCAGCTAT CGCATAGAGG CACACCCCAA CATCTGGTCA
    GATCGCAGTG AGCCTCTGAA GCTGATGGGG CCAGCAGGCT TTCTTACCTG GAATTACATT
    CTGAATGAAG CTATCAGATT GTCCCTAATC ATGCAGCTTG TTGCCTTGCT GTTGGTAGTG
    CTGTGGATAA GGTGGAAGTG TCGGAGACTC AGAATCAGAG AAGCCTGGTT GCTGGGAACA
    GCTCAAGGGG TCACCATGCT CTTCATAGTC ACGGCCCTTC TCTGCTGTGG ACTGTGCAAT
    GGGGTATTGA CAGAAGAGAC TGAAATAATC ATGCCAACCC CTAAGCCTGA GCTGTGGGCA
    GAGACCAACT TCCCTCTTGC CCCGTGGAAG AACTTAACCC TCTGGTGCAG AAGCCCTTCT
    GGCTCAACTA AGGAGTTTGT GTTGCTGAAG GACGGGACTG GGTGGATTGC AACTCGCCCG
    GCCTCCGAGC AGGTCCGGGC TGCCTTTCCC CTTGGCGCCC TAACCCAGAG CCATACCGGG
    AGTTACCACT GCCATTCATG GGAGGAGATG GCTGTGTCGG AGCCCAGTGA GGCACTTGAG
    CTGGTGGGGA CAGACATCCT CCCCAAACCT GTCATTTCTG CTTCCCCCCC AATTCGGGGC
    CAGAAACTGC AAATCCGGTG CAAAGGATGG CTGGCAGGCA TGGAGTTCGT TCTGTATAAG
    GAGGGAGTGC AGGAACCTGT CCAGCAACTT CATGCCGTTG GGAGAGAAGC CTTCTTTACA
    ATCCAAAGAA TGGAGGATAA AGACGAAGGC AATTATAGCT GCCGTACTCA TACTGAAAAG
    CGCCCCTTCA AGTGGTCTGA GCCCAGTGAT CCCATGGAGC TTGTCATAAA AGAAATGTAC
    CCCAAGCCCT TTTTCAAGAC ATGGGCCAGC CCTGTGGTCA CTCCTGGTGC CCGAGTGACT
    TTCAATTGCT CCACTCCCCA GCAGCACATG AGCTTTATTC TTTACAAAGA TGGAAGTGAA
    ATAGCATCCA GTGACAGGTC TTGGGCAAGT CCAGGGGCCA GTGCGGCTCA CTTTCTGATC
    ATTTCAGTGG GCACTGGTGA TGGAGGGAAT TATAGCTGCC GCTATTATGA CTTCGCTATC
    TGGTCTGAGC CCAGCGACCC TGTGGAGCTC GTGGTGACAG AATTCTACCC CAAACCTACT
    CTTCTGGCAC ACCCGGGTCC TGTGGTGCTT CCTGGAAAGA ATGTGACCCT GCGCTGCCAA
    GGGGCTTTCC AAGGCATGAG ATTTGCCCTC TTGCAGGAGG GAACCCAGGT TCCCTTACAG
    TTCCAGAGCA CCTCCGGGAA TTCAGCTGAC TTCCTCCTCC ACACTGTTAG AGCAGAGGAC
    TCTGGGAACT ACAGTTGTGT TTACTACGAG ACAACCATGT CAAATAGGGG ATCACATCTC
    AGCAAGCCCA TTATGATCTG GGTGACTGAC ACATTTCCCA AGCCATGGTT GTTTGCTGAG
    CCCAGTTCTG TGGTTCCTAT GGGGCAGAAT GTTACTCTCT GGTGCCAAGG GCCAGTCCAT
    GGAGTAGGAT ACATTCTGCA CAAAGAAAGA GAAACCACTT CAGTGCAGCT CTGGGGATCC
    ACCAGTAATG ATGGGGCATT CCCTATCACC AATATATCTG GTGCTAACAT AGGGCGTTAC
    AGCTGCTGCT ACCACCCTGA CTGGACCAGC CCTATCAAGA TACAGCCTAG CAACACTCTG
    GAACTCATAG TCACAGGTTT GCTCCCCAAA CCCAGCCTCT TAGCCCAGCC AGGTCCTATT
    GTGGCCCCTG GAGAAAATAT GACTTTTCAA TGCCAAGGGG AACTACCAGA CTCAACATTT
    GTCCTGTTGA AGGAAGGCAC TCAACAGCCT TTGGAACAAC AGAGCCCAAG TGGGTACAGG
    GCTGACTTCT GGATGCCAGC AGTGAGAGGT GATGATTCTG GGGTCTATAG CTGTGTTTAT
    TATTTGGACT CTGCTCCCTT TGCGGCTTCT AATCACAGTG ACTTCCTGGA GATCTGGGTG
    ACTGATAAGC CCCCTAAGCC CTCGCTGTCA GCCTGGCCCA GCACCATGTT CAAGCTAGGC
    AAGGACATCA CCCTTCAGTG CCGTGGCCCC CTGCCAGGTG TTGAATTTGT CCTGGAACAT
    GATGGAGAAG AAGCACCTCA GCAGTTCTCA GAGGATGGGG ACTTTGTCAT CAACAATGTA
    GAAGGAAAAG GCATTGGCAA CTACAGCTGC AGCTACCGCC TCCAGGCCTA TCCTGATATC
    TGGTCAGAGC CTAGTGATGC CCTGGAACTG GTGGGGGCAG CAGGGCCTGC TGCCCAGGAG
    TGCACTGTGG GGAACATTGT CCGAAGTAGT CTGATTGTGG TGGTAGTTGT AGCTTTGGGG
    GTAGTGCTAG CCATAGAATG GAAGAAGTGG CCTCGACTCC GAACCAGGGA CTCAGAGACA
    GATGGAAGAG ACCAGACCAT AGCCCTTGAA GAGTGTAACC AAGAAGGAGA ACCAGCCACC
    AACACCAGCT CTCCCTCCTC CGTCTCTCAG GGAACCTCAG TGGAACTGCC AGTCCCAGTA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45778
    Clone ID Related Accession (Same CDS sequence) XM_014111978.1
    Accession Version XM_014111978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3297bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product immunoglobulin superfamily member 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACCTG GAGAAAGCCT GAACCTCAGG TGTCAGGCAC CAATCTACGG AATGACCTTT 
    GCTCTAATAA GGCTTGAAGA TTTGAAGAAA TCCTTTTACC GCAAAAGGCC AATAAAAAAT
    GAGGCATATT TCTTCTTCCG GGCTTTGAAA ATCCATGATG CTGGACATTA CCTCTGTTTT
    TACTATGATG AGTCATACAG GGGTTCACTC CTTAGTGATA TCCTGAAAAT CTGGGTGACC
    GACACTTTCC CCAAGACCTG GCTACTTGCT CAGCCCAGTC CTGTGGTCCA GATGGGTCAG
    AACGTGAGCC TGTGGTGTCG AGGGCCAGTG GATGGAGTGG GGCTTGCACT CTATAAGAAA
    GGAGAAGACA AACCACTTCA GCTTTTGGAT ACCACCAGCA TTGATGACGA GTCATTCTTC
    CTCAACAATG TGACCTACAG TGATGCTGGC ATCTATAGTT GCCACTATCT CCTCTCCTGG
    AAGACCTCCA TCAGGATGAC ATCACACAAC ACTGTGGAAC TTGTGGTTGT AGATAAGCCC
    CCCAAACCCT CCCTTTCAGC CTGGCCCAGC ACCATGTTCA AGCTAAGAAA GGCCATTACC
    CTTCAGCACC GAGTACCCCA TCCAGTACTT GAATTTTCTC TGGAATGGGA AGAAAGAGCA
    ACATTCCAAA AATTCTTGGT AGATGGAGAT TTCATCATCA GTAATGCTGA AGGAAAAGGC
    ACAGAAACTT ACAGTTGCAG CTATCGCATA GAGGCACACC CCAACATCTG GTCAGATCGC
    AGTGAGCCTC TGAAGCTGAT GGGGCCAGCA GGCTTTCTTA CCTGGAATTA CATTCTGAAT
    GAAGCTATCA GATTGTCCCT AATCATGCAG CTTGTTGCCT TGCTGTTGGT AGTGCTGTGG
    ATAAGGTGGA AGTGTCGGAG ACTCAGAATC AGAGAAGCCT GGTTGCTGGG AACAGCTCAA
    GGGGTCACCA TGCTCTTCAT AGTCACGGCC CTTCTCTGCT GTGGACTGTG CAATGGGGTA
    TTGACAGAAG AGACTGAAAT AATCATGCCA ACCCCTAAGC CTGAGCTGTG GGCAGAGACC
    AACTTCCCTC TTGCCCCGTG GAAGAACTTA ACCCTCTGGT GCAGAAGCCC TTCTGGCTCA
    ACTAAGGAGT TTGTGTTGCT GAAGGACGGG ACTGGGTGGA TTGCAACTCG CCCGGCCTCC
    GAGCAGGTCC GGGCTGCCTT TCCCCTTGGC GCCCTAACCC AGAGCCATAC CGGGAGTTAC
    CACTGCCATT CATGGGAGGA GATGGCTGTG TCGGAGCCCA GTGAGGCACT TGAGCTGGTG
    GGGACAGACA TCCTCCCCAA ACCTGTCATT TCTGCTTCCC CCCCAATTCG GGGCCAGAAA
    CTGCAAATCC GGTGCAAAGG ATGGCTGGCA GGCATGGAGT TCGTTCTGTA TAAGGAGGGA
    GTGCAGGAAC CTGTCCAGCA ACTTCATGCC GTTGGGAGAG AAGCCTTCTT TACAATCCAA
    AGAATGGAGG ATAAAGACGA AGGCAATTAT AGCTGCCGTA CTCATACTGA AAAGCGCCCC
    TTCAAGTGGT CTGAGCCCAG TGATCCCATG GAGCTTGTCA TAAAAGAAAT GTACCCCAAG
    CCCTTTTTCA AGACATGGGC CAGCCCTGTG GTCACTCCTG GTGCCCGAGT GACTTTCAAT
    TGCTCCACTC CCCAGCAGCA CATGAGCTTT ATTCTTTACA AAGATGGAAG TGAAATAGCA
    TCCAGTGACA GGTCTTGGGC AAGTCCAGGG GCCAGTGCGG CTCACTTTCT GATCATTTCA
    GTGGGCACTG GTGATGGAGG GAATTATAGC TGCCGCTATT ATGACTTCGC TATCTGGTCT
    GAGCCCAGCG ACCCTGTGGA GCTCGTGGTG ACAGAATTCT ACCCCAAACC TACTCTTCTG
    GCACACCCGG GTCCTGTGGT GCTTCCTGGA AAGAATGTGA CCCTGCGCTG CCAAGGGGCT
    TTCCAAGGCA TGAGATTTGC CCTCTTGCAG GAGGGAACCC AGGTTCCCTT ACAGTTCCAG
    AGCACCTCCG GGAATTCAGC TGACTTCCTC CTCCACACTG TTAGAGCAGA GGACTCTGGG
    AACTACAGTT GTGTTTACTA CGAGACAACC ATGTCAAATA GGGGATCACA TCTCAGCAAG
    CCCATTATGA TCTGGGTGAC TGACACATTT CCCAAGCCAT GGTTGTTTGC TGAGCCCAGT
    TCTGTGGTTC CTATGGGGCA GAATGTTACT CTCTGGTGCC AAGGGCCAGT CCATGGAGTA
    GGATACATTC TGCACAAAGA AAGAGAAACC ACTTCAGTGC AGCTCTGGGG ATCCACCAGT
    AATGATGGGG CATTCCCTAT CACCAATATA TCTGGTGCTA ACATAGGGCG TTACAGCTGC
    TGCTACCACC CTGACTGGAC CAGCCCTATC AAGATACAGC CTAGCAACAC TCTGGAACTC
    ATAGTCACAG GTTTGCTCCC CAAACCCAGC CTCTTAGCCC AGCCAGGTCC TATTGTGGCC
    CCTGGAGAAA ATATGACTTT TCAATGCCAA GGGGAACTAC CAGACTCAAC ATTTGTCCTG
    TTGAAGGAAG GCACTCAACA GCCTTTGGAA CAACAGAGCC CAAGTGGGTA CAGGGCTGAC
    TTCTGGATGC CAGCAGTGAG AGGTGATGAT TCTGGGGTCT ATAGCTGTGT TTATTATTTG
    GACTCTGCTC CCTTTGCGGC TTCTAATCAC AGTGACTTCC TGGAGATCTG GGTGACTGAT
    AAGCCCCCTA AGCCCTCGCT GTCAGCCTGG CCCAGCACCA TGTTCAAGCT AGGCAAGGAC
    ATCACCCTTC AGTGCCGTGG CCCCCTGCCA GGTGTTGAAT TTGTCCTGGA ACATGATGGA
    GAAGAAGCAC CTCAGCAGTT CTCAGAGGAT GGGGACTTTG TCATCAACAA TGTAGAAGGA
    AAAGGCATTG GCAACTACAG CTGCAGCTAC CGCCTCCAGG CCTATCCTGA TATCTGGTCA
    GAGCCTAGTG ATGCCCTGGA ACTGGTGGGG GCAGCAGGGC CTGCTGCCCA GGAGTGCACT
    GTGGGGAACA TTGTCCGAAG TAGTCTGATT GTGGTGGTAG TTGTAGCTTT GGGGGTAGTG
    CTAGCCATAG AATGGAAGAA GTGGCCTCGA CTCCGAACCA GGGACTCAGA GACAGATGGA
    AGAGACCAGA CCATAGCCCT TGAAGAGTGT AACCAAGAAG GAGAACCAGC CACCAACACC
    AGCTCTCCCT CCTCCGTCTC TCAGGGAACC TCAGTGGAAC TGCCAGTCCC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967453.1
    CDS120..3416
    Translation

    Target ORF information:

    RefSeq Version XM_014111978.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris immunoglobulin superfamily, member 1 (IGSF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGCACCTG GAGAAAGCCT GAACCTCAGG TGTCAGGCAC CAATCTACGG AATGACCTTT 
    GCTCTAATAA GGCTTGAAGA TTTGAAGAAA TCCTTTTACC GCAAAAGGCC AATAAAAAAT
    GAGGCATATT TCTTCTTCCG GGCTTTGAAA ATCCATGATG CTGGACATTA CCTCTGTTTT
    TACTATGATG AGTCATACAG GGGTTCACTC CTTAGTGATA TCCTGAAAAT CTGGGTGACC
    GACACTTTCC CCAAGACCTG GCTACTTGCT CAGCCCAGTC CTGTGGTCCA GATGGGTCAG
    AACGTGAGCC TGTGGTGTCG AGGGCCAGTG GATGGAGTGG GGCTTGCACT CTATAAGAAA
    GGAGAAGACA AACCACTTCA GCTTTTGGAT ACCACCAGCA TTGATGACGA GTCATTCTTC
    CTCAACAATG TGACCTACAG TGATGCTGGC ATCTATAGTT GCCACTATCT CCTCTCCTGG
    AAGACCTCCA TCAGGATGAC ATCACACAAC ACTGTGGAAC TTGTGGTTGT AGATAAGCCC
    CCCAAACCCT CCCTTTCAGC CTGGCCCAGC ACCATGTTCA AGCTAAGAAA GGCCATTACC
    CTTCAGCACC GAGTACCCCA TCCAGTACTT GAATTTTCTC TGGAATGGGA AGAAAGAGCA
    ACATTCCAAA AATTCTTGGT AGATGGAGAT TTCATCATCA GTAATGCTGA AGGAAAAGGC
    ACAGAAACTT ACAGTTGCAG CTATCGCATA GAGGCACACC CCAACATCTG GTCAGATCGC
    AGTGAGCCTC TGAAGCTGAT GGGGCCAGCA GGCTTTCTTA CCTGGAATTA CATTCTGAAT
    GAAGCTATCA GATTGTCCCT AATCATGCAG CTTGTTGCCT TGCTGTTGGT AGTGCTGTGG
    ATAAGGTGGA AGTGTCGGAG ACTCAGAATC AGAGAAGCCT GGTTGCTGGG AACAGCTCAA
    GGGGTCACCA TGCTCTTCAT AGTCACGGCC CTTCTCTGCT GTGGACTGTG CAATGGGGTA
    TTGACAGAAG AGACTGAAAT AATCATGCCA ACCCCTAAGC CTGAGCTGTG GGCAGAGACC
    AACTTCCCTC TTGCCCCGTG GAAGAACTTA ACCCTCTGGT GCAGAAGCCC TTCTGGCTCA
    ACTAAGGAGT TTGTGTTGCT GAAGGACGGG ACTGGGTGGA TTGCAACTCG CCCGGCCTCC
    GAGCAGGTCC GGGCTGCCTT TCCCCTTGGC GCCCTAACCC AGAGCCATAC CGGGAGTTAC
    CACTGCCATT CATGGGAGGA GATGGCTGTG TCGGAGCCCA GTGAGGCACT TGAGCTGGTG
    GGGACAGACA TCCTCCCCAA ACCTGTCATT TCTGCTTCCC CCCCAATTCG GGGCCAGAAA
    CTGCAAATCC GGTGCAAAGG ATGGCTGGCA GGCATGGAGT TCGTTCTGTA TAAGGAGGGA
    GTGCAGGAAC CTGTCCAGCA ACTTCATGCC GTTGGGAGAG AAGCCTTCTT TACAATCCAA
    AGAATGGAGG ATAAAGACGA AGGCAATTAT AGCTGCCGTA CTCATACTGA AAAGCGCCCC
    TTCAAGTGGT CTGAGCCCAG TGATCCCATG GAGCTTGTCA TAAAAGAAAT GTACCCCAAG
    CCCTTTTTCA AGACATGGGC CAGCCCTGTG GTCACTCCTG GTGCCCGAGT GACTTTCAAT
    TGCTCCACTC CCCAGCAGCA CATGAGCTTT ATTCTTTACA AAGATGGAAG TGAAATAGCA
    TCCAGTGACA GGTCTTGGGC AAGTCCAGGG GCCAGTGCGG CTCACTTTCT GATCATTTCA
    GTGGGCACTG GTGATGGAGG GAATTATAGC TGCCGCTATT ATGACTTCGC TATCTGGTCT
    GAGCCCAGCG ACCCTGTGGA GCTCGTGGTG ACAGAATTCT ACCCCAAACC TACTCTTCTG
    GCACACCCGG GTCCTGTGGT GCTTCCTGGA AAGAATGTGA CCCTGCGCTG CCAAGGGGCT
    TTCCAAGGCA TGAGATTTGC CCTCTTGCAG GAGGGAACCC AGGTTCCCTT ACAGTTCCAG
    AGCACCTCCG GGAATTCAGC TGACTTCCTC CTCCACACTG TTAGAGCAGA GGACTCTGGG
    AACTACAGTT GTGTTTACTA CGAGACAACC ATGTCAAATA GGGGATCACA TCTCAGCAAG
    CCCATTATGA TCTGGGTGAC TGACACATTT CCCAAGCCAT GGTTGTTTGC TGAGCCCAGT
    TCTGTGGTTC CTATGGGGCA GAATGTTACT CTCTGGTGCC AAGGGCCAGT CCATGGAGTA
    GGATACATTC TGCACAAAGA AAGAGAAACC ACTTCAGTGC AGCTCTGGGG ATCCACCAGT
    AATGATGGGG CATTCCCTAT CACCAATATA TCTGGTGCTA ACATAGGGCG TTACAGCTGC
    TGCTACCACC CTGACTGGAC CAGCCCTATC AAGATACAGC CTAGCAACAC TCTGGAACTC
    ATAGTCACAG GTTTGCTCCC CAAACCCAGC CTCTTAGCCC AGCCAGGTCC TATTGTGGCC
    CCTGGAGAAA ATATGACTTT TCAATGCCAA GGGGAACTAC CAGACTCAAC ATTTGTCCTG
    TTGAAGGAAG GCACTCAACA GCCTTTGGAA CAACAGAGCC CAAGTGGGTA CAGGGCTGAC
    TTCTGGATGC CAGCAGTGAG AGGTGATGAT TCTGGGGTCT ATAGCTGTGT TTATTATTTG
    GACTCTGCTC CCTTTGCGGC TTCTAATCAC AGTGACTTCC TGGAGATCTG GGTGACTGAT
    AAGCCCCCTA AGCCCTCGCT GTCAGCCTGG CCCAGCACCA TGTTCAAGCT AGGCAAGGAC
    ATCACCCTTC AGTGCCGTGG CCCCCTGCCA GGTGTTGAAT TTGTCCTGGA ACATGATGGA
    GAAGAAGCAC CTCAGCAGTT CTCAGAGGAT GGGGACTTTG TCATCAACAA TGTAGAAGGA
    AAAGGCATTG GCAACTACAG CTGCAGCTAC CGCCTCCAGG CCTATCCTGA TATCTGGTCA
    GAGCCTAGTG ATGCCCTGGA ACTGGTGGGG GCAGCAGGGC CTGCTGCCCA GGAGTGCACT
    GTGGGGAACA TTGTCCGAAG TAGTCTGATT GTGGTGGTAG TTGTAGCTTT GGGGGTAGTG
    CTAGCCATAG AATGGAAGAA GTGGCCTCGA CTCCGAACCA GGGACTCAGA GACAGATGGA
    AGAGACCAGA CCATAGCCCT TGAAGAGTGT AACCAAGAAG GAGAACCAGC CACCAACACC
    AGCTCTCCCT CCTCCGTCTC TCAGGGAACC TCAGTGGAAC TGCCAGTCCC AGTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.