MED14 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED14
Entrez Gene ID	480884
Full Name	mediator complex subunit 14
Synonyms	CRSP2
General protein information	Preferred Names mediator complex subunit 14 Names mediator of RNA polymerase II transcription subunit 14 cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_538001.6	XP_538001.2	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_022415530.1	XP_022271238.1	mediator of RNA polymerase II transcription subunit 14 isoform X3
XM_022415531.1	XP_022271239.1	mediator of RNA polymerase II transcription subunit 14 isoform X4
XM_014111682.1	XP_013967157.1	mediator of RNA polymerase II transcription subunit 14 isoform X2
XM_538001.5	XP_538001.2	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_014111682.2	XP_013967157.1	mediator of RNA polymerase II transcription subunit 14 isoform X2
XM_022415532.1	XP_022271240.1	mediator of RNA polymerase II transcription subunit 14 isoform X5

Pathways from BioSystems

Homologies

	MED14	XP_001085867.1
Gallus gallus (chicken)	MED14	XP_416776.2
Xenopus tropicalis (tropical clawed frog)	med14	XP_002932839.1
Homo sapiens (human)	MED14	NP_004220.2
Bos taurus (cattle)	MED14	NP_001193960.1
Mus musculus (house mouse)	Med14	NP_001041673.1
Rattus norvegicus (Norway rat)	Med14	NP_001178656.1
Danio rerio (zebrafish)	med14	NP_997930.2
Anopheles gambiae (African malaria mosquito)	MED14_ANOGA	XP_315707.4
Drosophila melanogaster (fruit fly)	MED14	NP_612041.2
Pan troglodytes (chimpanzee)	MED14	XP_003317475.2
Canis lupus familiaris (dog)	MED14	XP_538001.2

Gene Ontology

The following MED14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe285086	XM_022415530.1 Latest version!	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe285088	XM_022415532.1 Latest version!	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe285087	XM_022415531.1 Latest version!	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe32255	XM_538001.6 Latest version!	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe45622	XM_014111682.2 Latest version!	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
OCe32255	XM_538001.5	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe45622	XM_014111682.1	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED14 ( XM_022415530.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271238.1 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe285086
Clone ID Related Accession (Same CDS sequence)	XM_022415530.1
Accession Version	XM_022415530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGG TGATTCAGGA GGCAGATAAG GACCTTTGTA AACATCATTT GAGACTGCCA 
AGGAAATCTG ATGTAGAAAG GAAAATAGAA ATAGTGCAGT TTGCTAGCCG GACACGTCAA 
CTCTTTGTTC GACTGTTAGC TTTAGTAAAA TGGGCTAATA ATGCTGGCAA GGTGGAAAAA 
TGTGCGATGA TTTCAAGCTT TTTAGATCAG CAAGCCATTC TGTTTGTGGA TACTGCTGAT 
CGCCTGGCCT CATTAGCTAG AGATGCCCTA GTCCATGCAC GCCTGCCTAG TTTTGCCATC 
CCGTATGCCA TTGATGTACT GACTACTGGG TCTTACCCAC GACTGCCCAC CTGCATTAGG 
GATAAAATTA TTCCTCCAGA CCCAATTACC AAGATTGAGA AACAAGCCAC ACTTCACCAA 
CTCAATCAGA TTCTTAGACA TCGGCTTGTG ACCACAGATC TTCCCCCTCA GTTAGCAAAT 
CTTACAGTTG CAAATGGCCG GGTGAAGTTT CGAGTTGAAG GAGAGTTTGA AGCCACCTTA 
ACCGTGATGG GAGATGATCC AGATGTTCCA TGGCGTCTTC TCAAGCTAGA AATTCTAGTT 
GAGGACAAGG AAACAGGAGA TGGGCGAGCC TTGGTTCATA GTATGCAAAT CAACTTTATC 
CATCAGCTGG TGCAGTCTAG ACTCTTTGCT GATGAAAAAC CTCTTCAGGA CATGTACAAC 
TGCCTACATT CTTTCTGCTT ATCGCTTCAG TTAGAAGTGC TACATTCCCA AACTCTAATG 
TTAATACGAG AGCGGTGGGG AGACCTTGTG CAGGTGGAAA GGTACCATGC TGGAAAGTGC 
CTCTCCCTCT CAGTTTGGAA TCAACAGGTT CTTGGGAGAA AAACAGGGAC GGCATCTGTT 
CACAAAGTTA CAATTAAAAT CGATGAAAAT GATGTCTCTA AGCCTTTACA GATTTTTCAT 
GATCCTCCTT TGCCAGCTTC TGATTCCAAA TTAGTAGAAA GAGCCATGAA GATTGACCAC 
TTATCAATAG AAAAGCTCCT GATAGACAGC GTCCATGCAC GAGCTCATCA GAAGCTCCAG 
GAGCTGAAGG CCATTCTGCG AGGCTTCAAT GCCAACGAAA ACTCTTCCAT AGAGACTGCG 
CTTCCGGCTC TTGTTGTTCC CATTTTGGAG CCCTGCGGTA ATTCTGAGTG TCTACACATT 
TTCGTAGACT TGCATTCTGG AATGTTTCAA TTGATGCTTT ACGGACTTGA CCAGGCCACG 
CTGGATGACA TGGAGAAGTC TGTGAATGAT GATATGAAAC GAATTATTCC TTGGATTCAA 
CAACTGAAGT TTTGGCTTGG ACAACAGCGT TGCAAACAAT CTATAAAACA TCTGCCTACA 
ATAAGCAGTG AAACATTGCA GCTGTCCAAT TACTCAACCC ATCCTATTGG AAATCTTTCT 
AAGAATAAGC TGTTCATTAA ACTTACCCGT CTTCCACAGT ACTACATTGT TGTGGAGATG 
TTGGAGGTTC CTAATAAACC CACACAGCTG TCATATAAGT ACTACTTCAT GTCTGTGAAT 
GCTGCAGAAC GTGAAGACAC CCCTGTTATG GCACTCCTGC TGCAGCAGTT CAAGGAAAAC 
GTCCAAGAAT TGGTTTTTCG CACAAAACCT GGGAAACAGC CTAGGACTGG TCCCAAGCGC 
AAGTTGTCTG ATGATCCATG TCCAGTAGAA CCCAAGAAAA CCAAACGATC AGGAGAAATG 
TGTGCCTTCA ATAAAGTTCT AGCTCACTTT GTTGCTATGT GTGATACAAA TATGCCATTT 
GTAGGACTTC GGTTGGAGCT GTCCAATCTG GAGATTCCCC ATCAAGGAGT ACAAATGGAA 
GGTGATGGCT TCAGCCATGC AATTCGCTTA CTGAAAATTC CTCCCTGTAA GGGTGTGAAT 
GAAGAAACCC AGAAAGCTCT GGATCGCTCT CTTCTCGATT GCACTTTCCG ATTGCAAGGT 
AGAAATAACC GCACCTGGGT GGCGGAGTTA GTATTTGCAA ATTGTCCACT TAATGGCACT 
TCTACTAGGG AGCAAGGGCC ATCCCGGCAT GTTTATCTGA CATATGAAAA CCTGTTGTCT 
GAACCCGTTG GTGGTAGGAA AGTGGTTGAA ATGTTCCTTA ATGACTGGAA TAGCATTGCA 
CGATTATATG AATGTGTGTT GGAATTTGCA CGTTCTCTAC CAGACATACC TGCTCATCTA 
AATATTTTCT CAGAAGTCCG TGTCTATAAT TACCGAAAAC TTATCTTGTG TTATGGAACC 
ACCAAGGGAA GCTCAATTAG CATCCAGTGG AATTCCATAC ATCAGAAATT TCACATTTCT 
TTGGGAACTG TTGGCCCAAA CTCAGGTTGC AGTAACTGTC ACAATACCAT TCTCCATCAG 
CTTCAAGAAA TGTTCAACAA AACCCCAAAT GTGGTTCAGT TATTACAGGT ACTCTTTGAT 
ACTCAGGCTC CGTTAAATGC CATCAACAAA CTCCCCACCG TGCCGATGTT GGGCTTGACC 
CAGAGAACCA ACACTGCTTA CCAGTGCTTC TCCATCCTGC CCCAATCGTC CACCCACATT 
AGACTGGCCT TCAGGAACAT GTACTGCATT GATATTTACT GCCGGAGCCG AGGAGTGGTG 
GCGATACGAG ATGGTGCCTA TAGTCTTTTT GATAATAGCA AGTTAGTGGA AGGTTTTTAT 
CCTGCACCCG GACTAAAGAC ATTCCTGAAT ATGTTTGTTG ACAGCAATCA GGATGCTCGA 
AGAAGGTCTG TAAATGAGGA CGATAATCCC CCTTCTCCCA TTGGAGGAGA TATGATGGAT 
TCTTTAATAT CACAGCTTCA ACCACCACCC CAACAGCAGC CATTTCCCAA GCAACCGGGA 
TCATCAGGTG CTTATCCCCT TACTTCACCC CCTACATCTT ACCACAGCAC AGTTAATCAG 
TCTCCCTCTA TGATGCACAC ACAGTCTCCA GGAAATCTGC ATGCTGCCAG CTCCCCCAGT 
GGGGCTTTGA GAGCCCCTTC ACCAGCGTCA TTTGTTCCAA CTCCTCCCCC ATCCTCGCAT 
GGAATCTCAA TAGGACCAGG GGCCAGTTTT GCTAGTCCAC ATGGAACCCT AGACCCTAGT 
TCTCCATACA CTATGGTGTC ACCAAGCGGA CGAGCTGGGA ACTGGCCAGG GTCTCCTCAA 
GTGTCTGGCC CCTCACCAGC GACACGCATG CCTGGAATGT CACCAGCCAA TCCATCACTA 
CATTCTCCAG TTCCAGATGC TTCTCATTCC CCTCGAGCTG GAACAAGTTC CCAAACTTTG 
CCAACAAACA TGCCTCCACC TCGTAAACTA CCTCAGCGCT CCTGGGCAGC CTCCATACCT 
ACCATCCTCA CACATAGTGC CTTGAACATT TTACTGCTGC CCTCTCCAAC ACCAGGCCTT 
GTACCTGGCC TGGCAGGTAG TTACCTTTGT TCTCCGCTTG AGAGATTCCT TGGATCGGTC 
ATCATGAGAC GGCACCTTCA AAGAATCATT CAACAAGAAA CGCTGCAGCT GATAAATTCC 
AATGAACCTG GAGTGATCAT GTTTAAAACT GATGCGCTGA AATGCAGAGT AGCCCTTAGT 
CCCAAAACCA ATCAAACACT TCAGCTAAAA GTGACACCTG AAAATGCAGG ACAGTGGAAA 
CCTGATGAAC TTCAAGTTTT GGAGAAATTC TTTGAAACAA GAGTTGCAGG ACCGCCATTT 
AAAGCCAACA CTTTAATAGC CTTCACCAAG CTGTTAGGAG CTCCTACACA CATCCTCAGG 
GACTGTGTGC ATATTATGAA GCTAGAGCTG TTTCCTGACC AGGCAACACA ACTAAAATGG 
AACGTCCAGT TTTGCCTGAC GATCCCTCCC AGTGCGCCTC CAATTGCACC CCCTGGGACA 
CCTGCTGTGG TGCTGAAATC CAAAATGCTA TTTTTTCTTC AGCTAACTCA GAAAACATCG 
GTCCCTCCCC AAGAACCTGT TAGTATTATA GTTCCGATCA TTTATGACAT GGCTTCAGGT 
ACAACCCAAC AGGCAGACAT TCCCAGACAG CAGAACTCTT CTGTTGCTGC TCCCATGATG 
GTCAGCAACA TTCTGAAGAG GTTTGCAGAG ATGAACCCAC CACGACAAGG TGAATGCACA 
ATATTTGCAG CTGTTCGTGA TTTAATGGCT AATCTTACAC TGCCCCCTGG TGGGCGTCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271238.1
CDS	701..4903
Translation	MNVVIQEADKDLCKHHLRLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_022415530.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415530.1

ORF Insert Sequence:

ATGAATGTGG TGATTCAGGA GGCAGATAAG GACCTTTGTA AACATCATTT GAGACTGCCA 
AGGAAATCTG ATGTAGAAAG GAAAATAGAA ATAGTGCAGT TTGCTAGCCG GACACGTCAA 
CTCTTTGTTC GACTGTTAGC TTTAGTAAAA TGGGCTAATA ATGCTGGCAA GGTGGAAAAA 
TGTGCGATGA TTTCAAGCTT TTTAGATCAG CAAGCCATTC TGTTTGTGGA TACTGCTGAT 
CGCCTGGCCT CATTAGCTAG AGATGCCCTA GTCCATGCAC GCCTGCCTAG TTTTGCCATC 
CCGTATGCCA TTGATGTACT GACTACTGGG TCTTACCCAC GACTGCCCAC CTGCATTAGG 
GATAAAATTA TTCCTCCAGA CCCAATTACC AAGATTGAGA AACAAGCCAC ACTTCACCAA 
CTCAATCAGA TTCTTAGACA TCGGCTTGTG ACCACAGATC TTCCCCCTCA GTTAGCAAAT 
CTTACAGTTG CAAATGGCCG GGTGAAGTTT CGAGTTGAAG GAGAGTTTGA AGCCACCTTA 
ACCGTGATGG GAGATGATCC AGATGTTCCA TGGCGTCTTC TCAAGCTAGA AATTCTAGTT 
GAGGACAAGG AAACAGGAGA TGGGCGAGCC TTGGTTCATA GTATGCAAAT CAACTTTATC 
CATCAGCTGG TGCAGTCTAG ACTCTTTGCT GATGAAAAAC CTCTTCAGGA CATGTACAAC 
TGCCTACATT CTTTCTGCTT ATCGCTTCAG TTAGAAGTGC TACATTCCCA AACTCTAATG 
TTAATACGAG AGCGGTGGGG AGACCTTGTG CAGGTGGAAA GGTACCATGC TGGAAAGTGC 
CTCTCCCTCT CAGTTTGGAA TCAACAGGTT CTTGGGAGAA AAACAGGGAC GGCATCTGTT 
CACAAAGTTA CAATTAAAAT CGATGAAAAT GATGTCTCTA AGCCTTTACA GATTTTTCAT 
GATCCTCCTT TGCCAGCTTC TGATTCCAAA TTAGTAGAAA GAGCCATGAA GATTGACCAC 
TTATCAATAG AAAAGCTCCT GATAGACAGC GTCCATGCAC GAGCTCATCA GAAGCTCCAG 
GAGCTGAAGG CCATTCTGCG AGGCTTCAAT GCCAACGAAA ACTCTTCCAT AGAGACTGCG 
CTTCCGGCTC TTGTTGTTCC CATTTTGGAG CCCTGCGGTA ATTCTGAGTG TCTACACATT 
TTCGTAGACT TGCATTCTGG AATGTTTCAA TTGATGCTTT ACGGACTTGA CCAGGCCACG 
CTGGATGACA TGGAGAAGTC TGTGAATGAT GATATGAAAC GAATTATTCC TTGGATTCAA 
CAACTGAAGT TTTGGCTTGG ACAACAGCGT TGCAAACAAT CTATAAAACA TCTGCCTACA 
ATAAGCAGTG AAACATTGCA GCTGTCCAAT TACTCAACCC ATCCTATTGG AAATCTTTCT 
AAGAATAAGC TGTTCATTAA ACTTACCCGT CTTCCACAGT ACTACATTGT TGTGGAGATG 
TTGGAGGTTC CTAATAAACC CACACAGCTG TCATATAAGT ACTACTTCAT GTCTGTGAAT 
GCTGCAGAAC GTGAAGACAC CCCTGTTATG GCACTCCTGC TGCAGCAGTT CAAGGAAAAC 
GTCCAAGAAT TGGTTTTTCG CACAAAACCT GGGAAACAGC CTAGGACTGG TCCCAAGCGC 
AAGTTGTCTG ATGATCCATG TCCAGTAGAA CCCAAGAAAA CCAAACGATC AGGAGAAATG 
TGTGCCTTCA ATAAAGTTCT AGCTCACTTT GTTGCTATGT GTGATACAAA TATGCCATTT 
GTAGGACTTC GGTTGGAGCT GTCCAATCTG GAGATTCCCC ATCAAGGAGT ACAAATGGAA 
GGTGATGGCT TCAGCCATGC AATTCGCTTA CTGAAAATTC CTCCCTGTAA GGGTGTGAAT 
GAAGAAACCC AGAAAGCTCT GGATCGCTCT CTTCTCGATT GCACTTTCCG ATTGCAAGGT 
AGAAATAACC GCACCTGGGT GGCGGAGTTA GTATTTGCAA ATTGTCCACT TAATGGCACT 
TCTACTAGGG AGCAAGGGCC ATCCCGGCAT GTTTATCTGA CATATGAAAA CCTGTTGTCT 
GAACCCGTTG GTGGTAGGAA AGTGGTTGAA ATGTTCCTTA ATGACTGGAA TAGCATTGCA 
CGATTATATG AATGTGTGTT GGAATTTGCA CGTTCTCTAC CAGACATACC TGCTCATCTA 
AATATTTTCT CAGAAGTCCG TGTCTATAAT TACCGAAAAC TTATCTTGTG TTATGGAACC 
ACCAAGGGAA GCTCAATTAG CATCCAGTGG AATTCCATAC ATCAGAAATT TCACATTTCT 
TTGGGAACTG TTGGCCCAAA CTCAGGTTGC AGTAACTGTC ACAATACCAT TCTCCATCAG 
CTTCAAGAAA TGTTCAACAA AACCCCAAAT GTGGTTCAGT TATTACAGGT ACTCTTTGAT 
ACTCAGGCTC CGTTAAATGC CATCAACAAA CTCCCCACCG TGCCGATGTT GGGCTTGACC 
CAGAGAACCA ACACTGCTTA CCAGTGCTTC TCCATCCTGC CCCAATCGTC CACCCACATT 
AGACTGGCCT TCAGGAACAT GTACTGCATT GATATTTACT GCCGGAGCCG AGGAGTGGTG 
GCGATACGAG ATGGTGCCTA TAGTCTTTTT GATAATAGCA AGTTAGTGGA AGGTTTTTAT 
CCTGCACCCG GACTAAAGAC ATTCCTGAAT ATGTTTGTTG ACAGCAATCA GGATGCTCGA 
AGAAGGTCTG TAAATGAGGA CGATAATCCC CCTTCTCCCA TTGGAGGAGA TATGATGGAT 
TCTTTAATAT CACAGCTTCA ACCACCACCC CAACAGCAGC CATTTCCCAA GCAACCGGGA 
TCATCAGGTG CTTATCCCCT TACTTCACCC CCTACATCTT ACCACAGCAC AGTTAATCAG 
TCTCCCTCTA TGATGCACAC ACAGTCTCCA GGAAATCTGC ATGCTGCCAG CTCCCCCAGT 
GGGGCTTTGA GAGCCCCTTC ACCAGCGTCA TTTGTTCCAA CTCCTCCCCC ATCCTCGCAT 
GGAATCTCAA TAGGACCAGG GGCCAGTTTT GCTAGTCCAC ATGGAACCCT AGACCCTAGT 
TCTCCATACA CTATGGTGTC ACCAAGCGGA CGAGCTGGGA ACTGGCCAGG GTCTCCTCAA 
GTGTCTGGCC CCTCACCAGC GACACGCATG CCTGGAATGT CACCAGCCAA TCCATCACTA 
CATTCTCCAG TTCCAGATGC TTCTCATTCC CCTCGAGCTG GAACAAGTTC CCAAACTTTG 
CCAACAAACA TGCCTCCACC TCGTAAACTA CCTCAGCGCT CCTGGGCAGC CTCCATACCT 
ACCATCCTCA CACATAGTGC CTTGAACATT TTACTGCTGC CCTCTCCAAC ACCAGGCCTT 
GTACCTGGCC TGGCAGGTAG TTACCTTTGT TCTCCGCTTG AGAGATTCCT TGGATCGGTC 
ATCATGAGAC GGCACCTTCA AAGAATCATT CAACAAGAAA CGCTGCAGCT GATAAATTCC 
AATGAACCTG GAGTGATCAT GTTTAAAACT GATGCGCTGA AATGCAGAGT AGCCCTTAGT 
CCCAAAACCA ATCAAACACT TCAGCTAAAA GTGACACCTG AAAATGCAGG ACAGTGGAAA 
CCTGATGAAC TTCAAGTTTT GGAGAAATTC TTTGAAACAA GAGTTGCAGG ACCGCCATTT 
AAAGCCAACA CTTTAATAGC CTTCACCAAG CTGTTAGGAG CTCCTACACA CATCCTCAGG 
GACTGTGTGC ATATTATGAA GCTAGAGCTG TTTCCTGACC AGGCAACACA ACTAAAATGG 
AACGTCCAGT TTTGCCTGAC GATCCCTCCC AGTGCGCCTC CAATTGCACC CCCTGGGACA 
CCTGCTGTGG TGCTGAAATC CAAAATGCTA TTTTTTCTTC AGCTAACTCA GAAAACATCG 
GTCCCTCCCC AAGAACCTGT TAGTATTATA GTTCCGATCA TTTATGACAT GGCTTCAGGT 
ACAACCCAAC AGGCAGACAT TCCCAGACAG CAGAACTCTT CTGTTGCTGC TCCCATGATG 
GTCAGCAACA TTCTGAAGAG GTTTGCAGAG ATGAACCCAC CACGACAAGG TGAATGCACA 
ATATTTGCAG CTGTTCGTGA TTTAATGGCT AATCTTACAC TGCCCCCTGG TGGGCGTCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_022415532.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271240.1 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe285088
Clone ID Related Accession (Same CDS sequence)	XM_022415532.1
Accession Version	XM_022415532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGACGGT GCCAGGTCTT AATGTACATT TTCTGTTTCT GCTGTGCTCC GGCCTTGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGTCAACTCT TTGTTCGACT GTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAGGTG GAAAAATGTG CGATGATTTC AAGCTTTTTA 
GATCAGCAAG CCATTCTGTT TGTGGATACT GCTGATCGCC TGGCCTCATT AGCTAGAGAT 
GCCCTAGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCGT ATGCCATTGA TGTACTGACT 
ACTGGGTCTT ACCCACGACT GCCCACCTGC ATTAGGGATA AAATTATTCC TCCAGACCCA 
ATTACCAAGA TTGAGAAACA AGCCACACTT CACCAACTCA ATCAGATTCT TAGACATCGG 
CTTGTGACCA CAGATCTTCC CCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGGGTG 
AAGTTTCGAG TTGAAGGAGA GTTTGAAGCC ACCTTAACCG TGATGGGAGA TGATCCAGAT 
GTTCCATGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ACAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAAATCAAC TTTATCCATC AGCTGGTGCA GTCTAGACTC 
TTTGCTGATG AAAAACCTCT TCAGGACATG TACAACTGCC TACATTCTTT CTGCTTATCG 
CTTCAGTTAG AAGTGCTACA TTCCCAAACT CTAATGTTAA TACGAGAGCG GTGGGGAGAC 
CTTGTGCAGG TGGAAAGGTA CCATGCTGGA AAGTGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACGGCA TCTGTTCACA AAGTTACAAT TAAAATCGAT 
GAAAATGATG TCTCTAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC AGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GACCACTTAT CAATAGAAAA GCTCCTGATA 
GACAGCGTCC ATGCACGAGC TCATCAGAAG CTCCAGGAGC TGAAGGCCAT TCTGCGAGGC 
TTCAATGCCA ACGAAAACTC TTCCATAGAG ACTGCGCTTC CGGCTCTTGT TGTTCCCATT 
TTGGAGCCCT GCGGTAATTC TGAGTGTCTA CACATTTTCG TAGACTTGCA TTCTGGAATG 
TTTCAATTGA TGCTTTACGG ACTTGACCAG GCCACGCTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGAAT TATTCCTTGG ATTCAACAAC TGAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACATCTG CCTACAATAA GCAGTGAAAC ATTGCAGCTG 
TCCAATTACT CAACCCATCC TATTGGAAAT CTTTCTAAGA ATAAGCTGTT CATTAAACTT 
ACCCGTCTTC CACAGTACTA CATTGTTGTG GAGATGTTGG AGGTTCCTAA TAAACCCACA 
CAGCTGTCAT ATAAGTACTA CTTCATGTCT GTGAATGCTG CAGAACGTGA AGACACCCCT 
GTTATGGCAC TCCTGCTGCA GCAGTTCAAG GAAAACGTCC AAGAATTGGT TTTTCGCACA 
AAACCTGGGA AACAGCCTAG GACTGGTCCC AAGCGCAAGT TGTCTGATGA TCCATGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGTGTG CCTTCAATAA AGTTCTAGCT 
CACTTTGTTG CTATGTGTGA TACAAATATG CCATTTGTAG GACTTCGGTT GGAGCTGTCC 
AATCTGGAGA TTCCCCATCA AGGAGTACAA ATGGAAGGTG ATGGCTTCAG CCATGCAATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT GTGAATGAAG AAACCCAGAA AGCTCTGGAT 
CGCTCTCTTC TCGATTGCAC TTTCCGATTG CAAGGTAGAA ATAACCGCAC CTGGGTGGCG 
GAGTTAGTAT TTGCAAATTG TCCACTTAAT GGCACTTCTA CTAGGGAGCA AGGGCCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGGAAAGTG 
GTTGAAATGT TCCTTAATGA CTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCTGCT CATCTAAATA TTTTCTCAGA AGTCCGTGTC 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGCTC AATTAGCATC 
CAGTGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTTGG CCCAAACTCA 
GGTTGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACC 
CCAAATGTGG TTCAGTTATT ACAGGTACTC TTTGATACTC AGGCTCCGTT AAATGCCATC 
AACAAACTCC CCACCGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCTTACCAG 
TGCTTCTCCA TCCTGCCCCA ATCGTCCACC CACATTAGAC TGGCCTTCAG GAACATGTAC 
TGCATTGATA TTTACTGCCG GAGCCGAGGA GTGGTGGCGA TACGAGATGG TGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTGGAAGGT TTTTATCCTG CACCCGGACT AAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATATCACA GCTTCAACCA 
CCACCCCAAC AGCAGCCATT TCCCAAGCAA CCGGGATCAT CAGGTGCTTA TCCCCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTT AATCAGTCTC CCTCTATGAT GCACACACAG 
TCTCCAGGAA ATCTGCATGC TGCCAGCTCC CCCAGTGGGG CTTTGAGAGC CCCTTCACCA 
GCGTCATTTG TTCCAACTCC TCCCCCATCC TCGCATGGAA TCTCAATAGG ACCAGGGGCC 
AGTTTTGCTA GTCCACATGG AACCCTAGAC CCTAGTTCTC CATACACTAT GGTGTCACCA 
AGCGGACGAG CTGGGAACTG GCCAGGGTCT CCTCAAGTGT CTGGCCCCTC ACCAGCGACA 
CGCATGCCTG GAATGTCACC AGCCAATCCA TCACTACATT CTCCAGTTCC AGATGCTTCT 
CATTCCCCTC GAGCTGGAAC AAGTTCCCAA ACTTTGCCAA CAAACATGCC TCCACCTCGT 
AAACTACCTC AGCGCTCCTG GGCAGCCTCC ATACCTACCA TCCTCACACA TAGTGCCTTG 
AACATTTTAC TGCTGCCCTC TCCAACACCA GGCCTTGTAC CTGGCCTGGC AGGTAGTTAC 
CTTTGTTCTC CGCTTGAGAG ATTCCTTGGA TCGGTCATCA TGAGACGGCA CCTTCAAAGA 
ATCATTCAAC AAGAAACGCT GCAGCTGATA AATTCCAATG AACCTGGAGT GATCATGTTT 
AAAACTGATG CGCTGAAATG CAGAGTAGCC CTTAGTCCCA AAACCAATCA AACACTTCAG 
CTAAAAGTGA CACCTGAAAA TGCAGGACAG TGGAAACCTG ATGAACTTCA AGTTTTGGAG 
AAATTCTTTG AAACAAGAGT TGCAGGACCG CCATTTAAAG CCAACACTTT AATAGCCTTC 
ACCAAGCTGT TAGGAGCTCC TACACACATC CTCAGGGACT GTGTGCATAT TATGAAGCTA 
GAGCTGTTTC CTGACCAGGC AACACAACTA AAATGGAACG TCCAGTTTTG CCTGACGATC 
CCTCCCAGTG CGCCTCCAAT TGCACCCCCT GGGACACCTG CTGTGGTGCT GAAATCCAAA 
ATGCTATTTT TTCTTCAGCT AACTCAGAAA ACATCGGTCC CTCCCCAAGA ACCTGTTAGT 
ATTATAGTTC CGATCATTTA TGACATGGCT TCAGGTACAA CCCAACAGGC AGACATTCCC 
AGACAGCAGA ACTCTTCTGT TGCTGCTCCC ATGATGGTCA GCAACATTCT GAAGAGGTTT 
GCAGAGATGA ACCCACCACG ACAAGGTGAA TGCACAATAT TTGCAGCTGT TCGTGATTTA 
ATGGCTAATC TTACACTGCC CCCTGGTGGG CGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271240.1
CDS	48..4226
Translation	MRRCQVLMYIFCFCCAPALKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_022415532.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415532.1

ORF Insert Sequence:

ATGAGACGGT GCCAGGTCTT AATGTACATT TTCTGTTTCT GCTGTGCTCC GGCCTTGAAA 
ATAGAAATAG TGCAGTTTGC TAGCCGGACA CGTCAACTCT TTGTTCGACT GTTAGCTTTA 
GTAAAATGGG CTAATAATGC TGGCAAGGTG GAAAAATGTG CGATGATTTC AAGCTTTTTA 
GATCAGCAAG CCATTCTGTT TGTGGATACT GCTGATCGCC TGGCCTCATT AGCTAGAGAT 
GCCCTAGTCC ATGCACGCCT GCCTAGTTTT GCCATCCCGT ATGCCATTGA TGTACTGACT 
ACTGGGTCTT ACCCACGACT GCCCACCTGC ATTAGGGATA AAATTATTCC TCCAGACCCA 
ATTACCAAGA TTGAGAAACA AGCCACACTT CACCAACTCA ATCAGATTCT TAGACATCGG 
CTTGTGACCA CAGATCTTCC CCCTCAGTTA GCAAATCTTA CAGTTGCAAA TGGCCGGGTG 
AAGTTTCGAG TTGAAGGAGA GTTTGAAGCC ACCTTAACCG TGATGGGAGA TGATCCAGAT 
GTTCCATGGC GTCTTCTCAA GCTAGAAATT CTAGTTGAGG ACAAGGAAAC AGGAGATGGG 
CGAGCCTTGG TTCATAGTAT GCAAATCAAC TTTATCCATC AGCTGGTGCA GTCTAGACTC 
TTTGCTGATG AAAAACCTCT TCAGGACATG TACAACTGCC TACATTCTTT CTGCTTATCG 
CTTCAGTTAG AAGTGCTACA TTCCCAAACT CTAATGTTAA TACGAGAGCG GTGGGGAGAC 
CTTGTGCAGG TGGAAAGGTA CCATGCTGGA AAGTGCCTCT CCCTCTCAGT TTGGAATCAA 
CAGGTTCTTG GGAGAAAAAC AGGGACGGCA TCTGTTCACA AAGTTACAAT TAAAATCGAT 
GAAAATGATG TCTCTAAGCC TTTACAGATT TTTCATGATC CTCCTTTGCC AGCTTCTGAT 
TCCAAATTAG TAGAAAGAGC CATGAAGATT GACCACTTAT CAATAGAAAA GCTCCTGATA 
GACAGCGTCC ATGCACGAGC TCATCAGAAG CTCCAGGAGC TGAAGGCCAT TCTGCGAGGC 
TTCAATGCCA ACGAAAACTC TTCCATAGAG ACTGCGCTTC CGGCTCTTGT TGTTCCCATT 
TTGGAGCCCT GCGGTAATTC TGAGTGTCTA CACATTTTCG TAGACTTGCA TTCTGGAATG 
TTTCAATTGA TGCTTTACGG ACTTGACCAG GCCACGCTGG ATGACATGGA GAAGTCTGTG 
AATGATGATA TGAAACGAAT TATTCCTTGG ATTCAACAAC TGAAGTTTTG GCTTGGACAA 
CAGCGTTGCA AACAATCTAT AAAACATCTG CCTACAATAA GCAGTGAAAC ATTGCAGCTG 
TCCAATTACT CAACCCATCC TATTGGAAAT CTTTCTAAGA ATAAGCTGTT CATTAAACTT 
ACCCGTCTTC CACAGTACTA CATTGTTGTG GAGATGTTGG AGGTTCCTAA TAAACCCACA 
CAGCTGTCAT ATAAGTACTA CTTCATGTCT GTGAATGCTG CAGAACGTGA AGACACCCCT 
GTTATGGCAC TCCTGCTGCA GCAGTTCAAG GAAAACGTCC AAGAATTGGT TTTTCGCACA 
AAACCTGGGA AACAGCCTAG GACTGGTCCC AAGCGCAAGT TGTCTGATGA TCCATGTCCA 
GTAGAACCCA AGAAAACCAA ACGATCAGGA GAAATGTGTG CCTTCAATAA AGTTCTAGCT 
CACTTTGTTG CTATGTGTGA TACAAATATG CCATTTGTAG GACTTCGGTT GGAGCTGTCC 
AATCTGGAGA TTCCCCATCA AGGAGTACAA ATGGAAGGTG ATGGCTTCAG CCATGCAATT 
CGCTTACTGA AAATTCCTCC CTGTAAGGGT GTGAATGAAG AAACCCAGAA AGCTCTGGAT 
CGCTCTCTTC TCGATTGCAC TTTCCGATTG CAAGGTAGAA ATAACCGCAC CTGGGTGGCG 
GAGTTAGTAT TTGCAAATTG TCCACTTAAT GGCACTTCTA CTAGGGAGCA AGGGCCATCC 
CGGCATGTTT ATCTGACATA TGAAAACCTG TTGTCTGAAC CCGTTGGTGG TAGGAAAGTG 
GTTGAAATGT TCCTTAATGA CTGGAATAGC ATTGCACGAT TATATGAATG TGTGTTGGAA 
TTTGCACGTT CTCTACCAGA CATACCTGCT CATCTAAATA TTTTCTCAGA AGTCCGTGTC 
TATAATTACC GAAAACTTAT CTTGTGTTAT GGAACCACCA AGGGAAGCTC AATTAGCATC 
CAGTGGAATT CCATACATCA GAAATTTCAC ATTTCTTTGG GAACTGTTGG CCCAAACTCA 
GGTTGCAGTA ACTGTCACAA TACCATTCTC CATCAGCTTC AAGAAATGTT CAACAAAACC 
CCAAATGTGG TTCAGTTATT ACAGGTACTC TTTGATACTC AGGCTCCGTT AAATGCCATC 
AACAAACTCC CCACCGTGCC GATGTTGGGC TTGACCCAGA GAACCAACAC TGCTTACCAG 
TGCTTCTCCA TCCTGCCCCA ATCGTCCACC CACATTAGAC TGGCCTTCAG GAACATGTAC 
TGCATTGATA TTTACTGCCG GAGCCGAGGA GTGGTGGCGA TACGAGATGG TGCCTATAGT 
CTTTTTGATA ATAGCAAGTT AGTGGAAGGT TTTTATCCTG CACCCGGACT AAAGACATTC 
CTGAATATGT TTGTTGACAG CAATCAGGAT GCTCGAAGAA GGTCTGTAAA TGAGGACGAT 
AATCCCCCTT CTCCCATTGG AGGAGATATG ATGGATTCTT TAATATCACA GCTTCAACCA 
CCACCCCAAC AGCAGCCATT TCCCAAGCAA CCGGGATCAT CAGGTGCTTA TCCCCTTACT 
TCACCCCCTA CATCTTACCA CAGCACAGTT AATCAGTCTC CCTCTATGAT GCACACACAG 
TCTCCAGGAA ATCTGCATGC TGCCAGCTCC CCCAGTGGGG CTTTGAGAGC CCCTTCACCA 
GCGTCATTTG TTCCAACTCC TCCCCCATCC TCGCATGGAA TCTCAATAGG ACCAGGGGCC 
AGTTTTGCTA GTCCACATGG AACCCTAGAC CCTAGTTCTC CATACACTAT GGTGTCACCA 
AGCGGACGAG CTGGGAACTG GCCAGGGTCT CCTCAAGTGT CTGGCCCCTC ACCAGCGACA 
CGCATGCCTG GAATGTCACC AGCCAATCCA TCACTACATT CTCCAGTTCC AGATGCTTCT 
CATTCCCCTC GAGCTGGAAC AAGTTCCCAA ACTTTGCCAA CAAACATGCC TCCACCTCGT 
AAACTACCTC AGCGCTCCTG GGCAGCCTCC ATACCTACCA TCCTCACACA TAGTGCCTTG 
AACATTTTAC TGCTGCCCTC TCCAACACCA GGCCTTGTAC CTGGCCTGGC AGGTAGTTAC 
CTTTGTTCTC CGCTTGAGAG ATTCCTTGGA TCGGTCATCA TGAGACGGCA CCTTCAAAGA 
ATCATTCAAC AAGAAACGCT GCAGCTGATA AATTCCAATG AACCTGGAGT GATCATGTTT 
AAAACTGATG CGCTGAAATG CAGAGTAGCC CTTAGTCCCA AAACCAATCA AACACTTCAG 
CTAAAAGTGA CACCTGAAAA TGCAGGACAG TGGAAACCTG ATGAACTTCA AGTTTTGGAG 
AAATTCTTTG AAACAAGAGT TGCAGGACCG CCATTTAAAG CCAACACTTT AATAGCCTTC 
ACCAAGCTGT TAGGAGCTCC TACACACATC CTCAGGGACT GTGTGCATAT TATGAAGCTA 
GAGCTGTTTC CTGACCAGGC AACACAACTA AAATGGAACG TCCAGTTTTG CCTGACGATC 
CCTCCCAGTG CGCCTCCAAT TGCACCCCCT GGGACACCTG CTGTGGTGCT GAAATCCAAA 
ATGCTATTTT TTCTTCAGCT AACTCAGAAA ACATCGGTCC CTCCCCAAGA ACCTGTTAGT 
ATTATAGTTC CGATCATTTA TGACATGGCT TCAGGTACAA CCCAACAGGC AGACATTCCC 
AGACAGCAGA ACTCTTCTGT TGCTGCTCCC ATGATGGTCA GCAACATTCT GAAGAGGTTT 
GCAGAGATGA ACCCACCACG ACAAGGTGAA TGCACAATAT TTGCAGCTGT TCGTGATTTA 
ATGGCTAATC TTACACTGCC CCCTGGTGGG CGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_022415531.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271239.1 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X4, mRNA.

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CloneID	OCe285087
Clone ID Related Accession (Same CDS sequence)	XM_022415531.1
Accession Version	XM_022415531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCT 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACC CTAGACCCTA GTTCTCCATA CACTATGGTG 
TCACCAAGCG GACGAGCTGG GAACTGGCCA GGGTCTCCTC AAGTGTCTGG CCCCTCACCA 
GCGACACGCA TGCCTGGAAT GTCACCAGCC AATCCATCAC TACATTCTCC AGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCCCAAACTT TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCCTGGGCA GCCTCCATAC CTACCATCCT CACACATAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACACCAGGCC TTGTACCTGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCGCT TGAGAGATTC CTTGGATCGG TCATCATGAG ACGGCACCTT 
CAAAGAATCA TTCAACAAGA AACGGTTGCA GGACCGCCAT TTAAAGCCAA CACTTTAATA 
GCCTTCACCA AGCTGTTAGG AGCTCCTACA CACATCCTCA GGGACTGTGT GCATATTATG 
AAGCTAGAGC TGTTTCCTGA CCAGGCAACA CAACTAAAAT GGAACGTCCA GTTTTGCCTG 
ACGATCCCTC CCAGTGCGCC TCCAATTGCA CCCCCTGGGA CACCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAGCTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCGAT CATTTATGAC ATGGCTTCAG GTACAACCCA ACAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAACCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271239.1
CDS	139..4323
Translation	MAPVQLENHQLVPPGGGGGASGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_022415531.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415531.1

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCT 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACC CTAGACCCTA GTTCTCCATA CACTATGGTG 
TCACCAAGCG GACGAGCTGG GAACTGGCCA GGGTCTCCTC AAGTGTCTGG CCCCTCACCA 
GCGACACGCA TGCCTGGAAT GTCACCAGCC AATCCATCAC TACATTCTCC AGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCCCAAACTT TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCCTGGGCA GCCTCCATAC CTACCATCCT CACACATAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACACCAGGCC TTGTACCTGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCGCT TGAGAGATTC CTTGGATCGG TCATCATGAG ACGGCACCTT 
CAAAGAATCA TTCAACAAGA AACGGTTGCA GGACCGCCAT TTAAAGCCAA CACTTTAATA 
GCCTTCACCA AGCTGTTAGG AGCTCCTACA CACATCCTCA GGGACTGTGT GCATATTATG 
AAGCTAGAGC TGTTTCCTGA CCAGGCAACA CAACTAAAAT GGAACGTCCA GTTTTGCCTG 
ACGATCCCTC CCAGTGCGCC TCCAATTGCA CCCCCTGGGA CACCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAGCTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCGAT CATTTATGAC ATGGCTTCAG GTACAACCCA ACAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAACCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_538001.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_538001.2 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

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CloneID	OCe32255
Clone ID Related Accession (Same CDS sequence)	XM_538001.6 , XM_538001.5
Accession Version	XM_538001.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_538001.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCT 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACC CTAGACCCTA GTTCTCCATA CACTATGGTG 
TCACCAAGCG GACGAGCTGG GAACTGGCCA GGGTCTCCTC AAGTGTCTGG CCCCTCACCA 
GCGACACGCA TGCCTGGAAT GTCACCAGCC AATCCATCAC TACATTCTCC AGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCCCAAACTT TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCCTGGGCA GCCTCCATAC CTACCATCCT CACACATAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACACCAGGCC TTGTACCTGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCGCT TGAGAGATTC CTTGGATCGG TCATCATGAG ACGGCACCTT 
CAAAGAATCA TTCAACAAGA AACGCTGCAG CTGATAAATT CCAATGAACC TGGAGTGATC 
ATGTTTAAAA CTGATGCGCT GAAATGCAGA GTAGCCCTTA GTCCCAAAAC CAATCAAACA 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCGCCAT TTAAAGCCAA CACTTTAATA 
GCCTTCACCA AGCTGTTAGG AGCTCCTACA CACATCCTCA GGGACTGTGT GCATATTATG 
AAGCTAGAGC TGTTTCCTGA CCAGGCAACA CAACTAAAAT GGAACGTCCA GTTTTGCCTG 
ACGATCCCTC CCAGTGCGCC TCCAATTGCA CCCCCTGGGA CACCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAGCTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCGAT CATTTATGAC ATGGCTTCAG GTACAACCCA ACAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAACCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_538001.2
CDS	134..4498
Translation	MAPVQLENHQLVPPGGGGGASGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_538001.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_538001.6

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCT 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACC CTAGACCCTA GTTCTCCATA CACTATGGTG 
TCACCAAGCG GACGAGCTGG GAACTGGCCA GGGTCTCCTC AAGTGTCTGG CCCCTCACCA 
GCGACACGCA TGCCTGGAAT GTCACCAGCC AATCCATCAC TACATTCTCC AGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCCCAAACTT TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCCTGGGCA GCCTCCATAC CTACCATCCT CACACATAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACACCAGGCC TTGTACCTGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCGCT TGAGAGATTC CTTGGATCGG TCATCATGAG ACGGCACCTT 
CAAAGAATCA TTCAACAAGA AACGCTGCAG CTGATAAATT CCAATGAACC TGGAGTGATC 
ATGTTTAAAA CTGATGCGCT GAAATGCAGA GTAGCCCTTA GTCCCAAAAC CAATCAAACA 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCGCCAT TTAAAGCCAA CACTTTAATA 
GCCTTCACCA AGCTGTTAGG AGCTCCTACA CACATCCTCA GGGACTGTGT GCATATTATG 
AAGCTAGAGC TGTTTCCTGA CCAGGCAACA CAACTAAAAT GGAACGTCCA GTTTTGCCTG 
ACGATCCCTC CCAGTGCGCC TCCAATTGCA CCCCCTGGGA CACCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAGCTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCGAT CATTTATGAC ATGGCTTCAG GTACAACCCA ACAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAACCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_538001.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_538001.2 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe32255
Clone ID Related Accession (Same CDS sequence)	XM_538001.6 , XM_538001.5
Accession Version	XM_538001.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_538001.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCT 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACC CTAGACCCTA GTTCTCCATA CACTATGGTG 
TCACCAAGCG GACGAGCTGG GAACTGGCCA GGGTCTCCTC AAGTGTCTGG CCCCTCACCA 
GCGACACGCA TGCCTGGAAT GTCACCAGCC AATCCATCAC TACATTCTCC AGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCCCAAACTT TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCCTGGGCA GCCTCCATAC CTACCATCCT CACACATAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACACCAGGCC TTGTACCTGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCGCT TGAGAGATTC CTTGGATCGG TCATCATGAG ACGGCACCTT 
CAAAGAATCA TTCAACAAGA AACGCTGCAG CTGATAAATT CCAATGAACC TGGAGTGATC 
ATGTTTAAAA CTGATGCGCT GAAATGCAGA GTAGCCCTTA GTCCCAAAAC CAATCAAACA 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCGCCAT TTAAAGCCAA CACTTTAATA 
GCCTTCACCA AGCTGTTAGG AGCTCCTACA CACATCCTCA GGGACTGTGT GCATATTATG 
AAGCTAGAGC TGTTTCCTGA CCAGGCAACA CAACTAAAAT GGAACGTCCA GTTTTGCCTG 
ACGATCCCTC CCAGTGCGCC TCCAATTGCA CCCCCTGGGA CACCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAGCTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCGAT CATTTATGAC ATGGCTTCAG GTACAACCCA ACAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAACCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_538001.2
CDS	132..4496
Translation	MAPVQLENHQLVPPGGGGGASGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_538001.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_538001.5

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCT 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACC CTAGACCCTA GTTCTCCATA CACTATGGTG 
TCACCAAGCG GACGAGCTGG GAACTGGCCA GGGTCTCCTC AAGTGTCTGG CCCCTCACCA 
GCGACACGCA TGCCTGGAAT GTCACCAGCC AATCCATCAC TACATTCTCC AGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCCCAAACTT TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCCTGGGCA GCCTCCATAC CTACCATCCT CACACATAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACACCAGGCC TTGTACCTGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCGCT TGAGAGATTC CTTGGATCGG TCATCATGAG ACGGCACCTT 
CAAAGAATCA TTCAACAAGA AACGCTGCAG CTGATAAATT CCAATGAACC TGGAGTGATC 
ATGTTTAAAA CTGATGCGCT GAAATGCAGA GTAGCCCTTA GTCCCAAAAC CAATCAAACA 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCGCCAT TTAAAGCCAA CACTTTAATA 
GCCTTCACCA AGCTGTTAGG AGCTCCTACA CACATCCTCA GGGACTGTGT GCATATTATG 
AAGCTAGAGC TGTTTCCTGA CCAGGCAACA CAACTAAAAT GGAACGTCCA GTTTTGCCTG 
ACGATCCCTC CCAGTGCGCC TCCAATTGCA CCCCCTGGGA CACCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAGCTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCGAT CATTTATGAC ATGGCTTCAG GTACAACCCA ACAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAACCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_014111682.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967157.1 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45622
Clone ID Related Accession (Same CDS sequence)	XM_014111682.1 , XM_014111682.2
Accession Version	XM_014111682.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAACCCT AGACCCTAGT TCTCCATACA CTATGGTGTC ACCAAGCGGA 
CGAGCTGGGA ACTGGCCAGG GTCTCCTCAA GTGTCTGGCC CCTCACCAGC GACACGCATG 
CCTGGAATGT CACCAGCCAA TCCATCACTA CATTCTCCAG TTCCAGATGC TTCTCATTCC 
CCTCGAGCTG GAACAAGTTC CCAAACTTTG CCAACAAACA TGCCTCCACC TCGTAAACTA 
CCTCAGCGCT CCTGGGCAGC CTCCATACCT ACCATCCTCA CACATAGTGC CTTGAACATT 
TTACTGCTGC CCTCTCCAAC ACCAGGCCTT GTACCTGGCC TGGCAGGTAG TTACCTTTGT 
TCTCCGCTTG AGAGATTCCT TGGATCGGTC ATCATGAGAC GGCACCTTCA AAGAATCATT 
CAACAAGAAA CGCTGCAGCT GATAAATTCC AATGAACCTG GAGTGATCAT GTTTAAAACT 
GATGCGCTGA AATGCAGAGT AGCCCTTAGT CCCAAAACCA ATCAAACACT TCAGCTAAAA 
GTGACACCTG AAAATGCAGG ACAGTGGAAA CCTGATGAAC TTCAAGTTTT GGAGAAATTC 
TTTGAAACAA GAGTTGCAGG ACCGCCATTT AAAGCCAACA CTTTAATAGC CTTCACCAAG 
CTGTTAGGAG CTCCTACACA CATCCTCAGG GACTGTGTGC ATATTATGAA GCTAGAGCTG 
TTTCCTGACC AGGCAACACA ACTAAAATGG AACGTCCAGT TTTGCCTGAC GATCCCTCCC 
AGTGCGCCTC CAATTGCACC CCCTGGGACA CCTGCTGTGG TGCTGAAATC CAAAATGCTA 
TTTTTTCTTC AGCTAACTCA GAAAACATCG GTCCCTCCCC AAGAACCTGT TAGTATTATA 
GTTCCGATCA TTTATGACAT GGCTTCAGGT ACAACCCAAC AGGCAGACAT TCCCAGACAG 
CAGAACTCTT CTGTTGCTGC TCCCATGATG GTCAGCAACA TTCTGAAGAG GTTTGCAGAG 
ATGAACCCAC CACGACAAGG TGAATGCACA ATATTTGCAG CTGTTCGTGA TTTAATGGCT 
AATCTTACAC TGCCCCCTGG TGGGCGTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967157.1
CDS	135..4367
Translation	MAPVQLENHQLVPPGGGGGASGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_014111682.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111682.1

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAACCCT AGACCCTAGT TCTCCATACA CTATGGTGTC ACCAAGCGGA 
CGAGCTGGGA ACTGGCCAGG GTCTCCTCAA GTGTCTGGCC CCTCACCAGC GACACGCATG 
CCTGGAATGT CACCAGCCAA TCCATCACTA CATTCTCCAG TTCCAGATGC TTCTCATTCC 
CCTCGAGCTG GAACAAGTTC CCAAACTTTG CCAACAAACA TGCCTCCACC TCGTAAACTA 
CCTCAGCGCT CCTGGGCAGC CTCCATACCT ACCATCCTCA CACATAGTGC CTTGAACATT 
TTACTGCTGC CCTCTCCAAC ACCAGGCCTT GTACCTGGCC TGGCAGGTAG TTACCTTTGT 
TCTCCGCTTG AGAGATTCCT TGGATCGGTC ATCATGAGAC GGCACCTTCA AAGAATCATT 
CAACAAGAAA CGCTGCAGCT GATAAATTCC AATGAACCTG GAGTGATCAT GTTTAAAACT 
GATGCGCTGA AATGCAGAGT AGCCCTTAGT CCCAAAACCA ATCAAACACT TCAGCTAAAA 
GTGACACCTG AAAATGCAGG ACAGTGGAAA CCTGATGAAC TTCAAGTTTT GGAGAAATTC 
TTTGAAACAA GAGTTGCAGG ACCGCCATTT AAAGCCAACA CTTTAATAGC CTTCACCAAG 
CTGTTAGGAG CTCCTACACA CATCCTCAGG GACTGTGTGC ATATTATGAA GCTAGAGCTG 
TTTCCTGACC AGGCAACACA ACTAAAATGG AACGTCCAGT TTTGCCTGAC GATCCCTCCC 
AGTGCGCCTC CAATTGCACC CCCTGGGACA CCTGCTGTGG TGCTGAAATC CAAAATGCTA 
TTTTTTCTTC AGCTAACTCA GAAAACATCG GTCCCTCCCC AAGAACCTGT TAGTATTATA 
GTTCCGATCA TTTATGACAT GGCTTCAGGT ACAACCCAAC AGGCAGACAT TCCCAGACAG 
CAGAACTCTT CTGTTGCTGC TCCCATGATG GTCAGCAACA TTCTGAAGAG GTTTGCAGAG 
ATGAACCCAC CACGACAAGG TGAATGCACA ATATTTGCAG CTGTTCGTGA TTTAATGGCT 
AATCTTACAC TGCCCCCTGG TGGGCGTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_014111682.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967157.1 Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

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CloneID	OCe45622
Clone ID Related Accession (Same CDS sequence)	XM_014111682.1 , XM_014111682.2
Accession Version	XM_014111682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAACCCT AGACCCTAGT TCTCCATACA CTATGGTGTC ACCAAGCGGA 
CGAGCTGGGA ACTGGCCAGG GTCTCCTCAA GTGTCTGGCC CCTCACCAGC GACACGCATG 
CCTGGAATGT CACCAGCCAA TCCATCACTA CATTCTCCAG TTCCAGATGC TTCTCATTCC 
CCTCGAGCTG GAACAAGTTC CCAAACTTTG CCAACAAACA TGCCTCCACC TCGTAAACTA 
CCTCAGCGCT CCTGGGCAGC CTCCATACCT ACCATCCTCA CACATAGTGC CTTGAACATT 
TTACTGCTGC CCTCTCCAAC ACCAGGCCTT GTACCTGGCC TGGCAGGTAG TTACCTTTGT 
TCTCCGCTTG AGAGATTCCT TGGATCGGTC ATCATGAGAC GGCACCTTCA AAGAATCATT 
CAACAAGAAA CGCTGCAGCT GATAAATTCC AATGAACCTG GAGTGATCAT GTTTAAAACT 
GATGCGCTGA AATGCAGAGT AGCCCTTAGT CCCAAAACCA ATCAAACACT TCAGCTAAAA 
GTGACACCTG AAAATGCAGG ACAGTGGAAA CCTGATGAAC TTCAAGTTTT GGAGAAATTC 
TTTGAAACAA GAGTTGCAGG ACCGCCATTT AAAGCCAACA CTTTAATAGC CTTCACCAAG 
CTGTTAGGAG CTCCTACACA CATCCTCAGG GACTGTGTGC ATATTATGAA GCTAGAGCTG 
TTTCCTGACC AGGCAACACA ACTAAAATGG AACGTCCAGT TTTGCCTGAC GATCCCTCCC 
AGTGCGCCTC CAATTGCACC CCCTGGGACA CCTGCTGTGG TGCTGAAATC CAAAATGCTA 
TTTTTTCTTC AGCTAACTCA GAAAACATCG GTCCCTCCCC AAGAACCTGT TAGTATTATA 
GTTCCGATCA TTTATGACAT GGCTTCAGGT ACAACCCAAC AGGCAGACAT TCCCAGACAG 
CAGAACTCTT CTGTTGCTGC TCCCATGATG GTCAGCAACA TTCTGAAGAG GTTTGCAGAG 
ATGAACCCAC CACGACAAGG TGAATGCACA ATATTTGCAG CTGTTCGTGA TTTAATGGCT 
AATCTTACAC TGCCCCCTGG TGGGCGTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967157.1
CDS	135..4367
Translation	MAPVQLENHQLVPPGGGGGASGGPASAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQINFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAAEREDTPVMALLLQQFKENVQELVFRTKPGKQPRTGPKRKLSDDPCPVEPKKTKRSGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQMEGDGFSHAIRLLKIPPCKGVNEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGSSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPATRMPGMSPANPSLHSPVPDASHSPRAGTSSQTLPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_014111682.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111682.2

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG TTGGTCCCGC CTGGAGGCGG TGGCGGGGCC 
AGCGGTGGTC CCGCGTCAGC CCCTGCGCCT CCTCCTCCAG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCCG CGGCCAGCCC GGGCTACCGG CTGAGCACCC TCATCGAATT TCTCCTGCAC 
CGGGCCTACT CCGAGCTCAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTAGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGTC AACTCTTTGT TCGACTGTTA 
GCTTTAGTAA AATGGGCTAA TAATGCTGGC AAGGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT TCTGTTTGTG GATACTGCTG ATCGCCTGGC CTCATTAGCT 
AGAGATGCCC TAGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCGTATGC CATTGATGTA 
CTGACTACTG GGTCTTACCC ACGACTGCCC ACCTGCATTA GGGATAAAAT TATTCCTCCA 
GACCCAATTA CCAAGATTGA GAAACAAGCC ACACTTCACC AACTCAATCA GATTCTTAGA 
CATCGGCTTG TGACCACAGA TCTTCCCCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGAGTTGA AGGAGAGTTT GAAGCCACCT TAACCGTGAT GGGAGATGAT 
CCAGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGACAA GGAAACAGGA 
GATGGGCGAG CCTTGGTTCA TAGTATGCAA ATCAACTTTA TCCATCAGCT GGTGCAGTCT 
AGACTCTTTG CTGATGAAAA ACCTCTTCAG GACATGTACA ACTGCCTACA TTCTTTCTGC 
TTATCGCTTC AGTTAGAAGT GCTACATTCC CAAACTCTAA TGTTAATACG AGAGCGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTACCAT GCTGGAAAGT GCCTCTCCCT CTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACAGGG ACGGCATCTG TTCACAAAGT TACAATTAAA 
ATCGATGAAA ATGATGTCTC TAAGCCTTTA CAGATTTTTC ATGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATTGACC ACTTATCAAT AGAAAAGCTC 
CTGATAGACA GCGTCCATGC ACGAGCTCAT CAGAAGCTCC AGGAGCTGAA GGCCATTCTG 
CGAGGCTTCA ATGCCAACGA AAACTCTTCC ATAGAGACTG CGCTTCCGGC TCTTGTTGTT 
CCCATTTTGG AGCCCTGCGG TAATTCTGAG TGTCTACACA TTTTCGTAGA CTTGCATTCT 
GGAATGTTTC AATTGATGCT TTACGGACTT GACCAGGCCA CGCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATTATT CCTTGGATTC AACAACTGAA GTTTTGGCTT 
GGACAACAGC GTTGCAAACA ATCTATAAAA CATCTGCCTA CAATAAGCAG TGAAACATTG 
CAGCTGTCCA ATTACTCAAC CCATCCTATT GGAAATCTTT CTAAGAATAA GCTGTTCATT 
AAACTTACCC GTCTTCCACA GTACTACATT GTTGTGGAGA TGTTGGAGGT TCCTAATAAA 
CCCACACAGC TGTCATATAA GTACTACTTC ATGTCTGTGA ATGCTGCAGA ACGTGAAGAC 
ACCCCTGTTA TGGCACTCCT GCTGCAGCAG TTCAAGGAAA ACGTCCAAGA ATTGGTTTTT 
CGCACAAAAC CTGGGAAACA GCCTAGGACT GGTCCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AACCCAAGAA AACCAAACGA TCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
CTAGCTCACT TTGTTGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
CTGTCCAATC TGGAGATTCC CCATCAAGGA GTACAAATGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TACTGAAAAT TCCTCCCTGT AAGGGTGTGA ATGAAGAAAC CCAGAAAGCT 
CTGGATCGCT CTCTTCTCGA TTGCACTTTC CGATTGCAAG GTAGAAATAA CCGCACCTGG 
GTGGCGGAGT TAGTATTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGG 
CCATCCCGGC ATGTTTATCT GACATATGAA AACCTGTTGT CTGAACCCGT TGGTGGTAGG 
AAAGTGGTTG AAATGTTCCT TAATGACTGG AATAGCATTG CACGATTATA TGAATGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTC 
CGTGTCTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGCATCCAGT GGAATTCCAT ACATCAGAAA TTTCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACCCCAA ATGTGGTTCA GTTATTACAG GTACTCTTTG ATACTCAGGC TCCGTTAAAT 
GCCATCAACA AACTCCCCAC CGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAACACTGCT 
TACCAGTGCT TCTCCATCCT GCCCCAATCG TCCACCCACA TTAGACTGGC CTTCAGGAAC 
ATGTACTGCA TTGATATTTA CTGCCGGAGC CGAGGAGTGG TGGCGATACG AGATGGTGCC 
TATAGTCTTT TTGATAATAG CAAGTTAGTG GAAGGTTTTT ATCCTGCACC CGGACTAAAG 
ACATTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC CATTGGAGGA GATATGATGG ATTCTTTAAT ATCACAGCTT 
CAACCACCAC CCCAACAGCA GCCATTTCCC AAGCAACCGG GATCATCAGG TGCTTATCCC 
CTTACTTCAC CCCCTACATC TTACCACAGC ACAGTTAATC AGTCTCCCTC TATGATGCAC 
ACACAGTCTC CAGGAACCCT AGACCCTAGT TCTCCATACA CTATGGTGTC ACCAAGCGGA 
CGAGCTGGGA ACTGGCCAGG GTCTCCTCAA GTGTCTGGCC CCTCACCAGC GACACGCATG 
CCTGGAATGT CACCAGCCAA TCCATCACTA CATTCTCCAG TTCCAGATGC TTCTCATTCC 
CCTCGAGCTG GAACAAGTTC CCAAACTTTG CCAACAAACA TGCCTCCACC TCGTAAACTA 
CCTCAGCGCT CCTGGGCAGC CTCCATACCT ACCATCCTCA CACATAGTGC CTTGAACATT 
TTACTGCTGC CCTCTCCAAC ACCAGGCCTT GTACCTGGCC TGGCAGGTAG TTACCTTTGT 
TCTCCGCTTG AGAGATTCCT TGGATCGGTC ATCATGAGAC GGCACCTTCA AAGAATCATT 
CAACAAGAAA CGCTGCAGCT GATAAATTCC AATGAACCTG GAGTGATCAT GTTTAAAACT 
GATGCGCTGA AATGCAGAGT AGCCCTTAGT CCCAAAACCA ATCAAACACT TCAGCTAAAA 
GTGACACCTG AAAATGCAGG ACAGTGGAAA CCTGATGAAC TTCAAGTTTT GGAGAAATTC 
TTTGAAACAA GAGTTGCAGG ACCGCCATTT AAAGCCAACA CTTTAATAGC CTTCACCAAG 
CTGTTAGGAG CTCCTACACA CATCCTCAGG GACTGTGTGC ATATTATGAA GCTAGAGCTG 
TTTCCTGACC AGGCAACACA ACTAAAATGG AACGTCCAGT TTTGCCTGAC GATCCCTCCC 
AGTGCGCCTC CAATTGCACC CCCTGGGACA CCTGCTGTGG TGCTGAAATC CAAAATGCTA 
TTTTTTCTTC AGCTAACTCA GAAAACATCG GTCCCTCCCC AAGAACCTGT TAGTATTATA 
GTTCCGATCA TTTATGACAT GGCTTCAGGT ACAACCCAAC AGGCAGACAT TCCCAGACAG 
CAGAACTCTT CTGTTGCTGC TCCCATGATG GTCAGCAACA TTCTGAAGAG GTTTGCAGAG 
ATGAACCCAC CACGACAAGG TGAATGCACA ATATTTGCAG CTGTTCGTGA TTTAATGGCT 
AATCTTACAC TGCCCCCTGG TGGGCGTCCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

MED14 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報