ATG2B gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_014116145.2	XP_013971620.1	autophagy-related protein 2 homolog B isoform X2
XM_537549.5	XP_537549.3	autophagy-related protein 2 homolog B isoform X4
XM_537549.4	XP_537549.3	autophagy-related protein 2 homolog B isoform X3
XM_014116146.1	XP_013971621.1	autophagy-related protein 2 homolog B isoform X4
XM_022422359.1	XP_022278067.1	autophagy-related protein 2 homolog B isoform X5
XM_014116144.1	XP_013971619.1	autophagy-related protein 2 homolog B isoform X1
XM_014116145.1	XP_013971620.1	autophagy-related protein 2 homolog B isoform X2
XM_014116146.2	XP_013971621.1	autophagy-related protein 2 homolog B isoform X6
XM_014116147.1	XP_013971622.1	autophagy-related protein 2 homolog B isoform X5
XM_022422358.1	XP_022278066.1	autophagy-related protein 2 homolog B isoform X3
XM_014116144.2	XP_013971619.1	autophagy-related protein 2 homolog B isoform X1
XM_022422360.1	XP_022278068.1	autophagy-related protein 2 homolog B isoform X7
XM_014116147.2	XP_013971622.1	autophagy-related protein 2 homolog B isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe290272	XM_022422360.1 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe38047	XM_014116146.2 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe38048	XM_014116147.2 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe290270	XM_022422358.1 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290271	XM_022422359.1 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe38045	XM_014116144.2 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe38046	XM_014116145.2 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe10801	XM_537549.5 Latest version!	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe10801	XM_537549.4	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe38046	XM_014116145.1	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe38047	XM_014116146.1	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe38045	XM_014116144.1	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe38048	XM_014116147.1	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

ATG2B ( XM_022422360.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022278068.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe290272
Clone ID Related Accession (Same CDS sequence)	XM_022422360.1
Accession Version	XM_022422360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GTTTGGAAAC TTTATATAAA TATACTCTAT 
AAGATTATCT CCCTTGTTTT CTCTAATGGA GTTAATTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278068.1
CDS	16..5637
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQVWKLYINILYKIISLVFSNGVNF

Target ORF information:

RefSeq Version	XM_022422360.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022422360.1

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GTTTGGAAAC TTTATATAAA TATACTCTAT 
AAGATTATCT CCCTTGTTTT CTCTAATGGA GTTAATTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116146.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971621.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X4, mRNA.

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CloneID	OCe38047
Clone ID Related Accession (Same CDS sequence)	XM_014116146.1 , XM_014116146.2
Accession Version	XM_014116146.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	autophagy-related protein 2 homolog B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGTTATTTG 
CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971621.1
CDS	2..5647
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGYLL

Target ORF information:

RefSeq Version	XM_014116146.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116146.1

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGTTATTTG 
CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116146.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971621.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe38047
Clone ID Related Accession (Same CDS sequence)	XM_014116146.1 , XM_014116146.2
Accession Version	XM_014116146.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116146.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGTTATTTG 
CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971621.1
CDS	16..5661
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGYLL

Target ORF information:

RefSeq Version	XM_014116146.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116146.2

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGTTATTTG 
CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116147.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971622.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe38048
Clone ID Related Accession (Same CDS sequence)	XM_014116147.1 , XM_014116147.2
Accession Version	XM_014116147.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	autophagy-related protein 2 homolog B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGG AAGACGAATA TCGAATTCAG ATGGAATTAA ACCGGTATTA TTTGAGAAAA 
GATTCCCTTT CGGTGGGTGT GTCTTCAGAG CAAAGTTTTT ATGAGACAGA ATCTGCTCGT 
ACACCTTCTA GCCGTGAAGA AGAAGTTTTC TTCTCCATGG CTGATATGGA CATGTCTCAT 
AGTCTCTCTT CTCTTCCACC TCTGGGAGAC CCTCCAAATA TGGACCTTGA GTTATCATTA 
ACTAGTACAT ACACAAATAC TCCAGCAGGA TCTCCATTAA GTGCTACTGT GCTTCAGCCA 
ACGTGGGGAG ATTTCCTTGA TCATCATAAA GAACAACCAG TAAGAGGGTC CACCTTGCCA 
TCCAACCTAG TTCACCCACC AAATTTACAG AAGACTTCTC TCCCATCTAG ATCTGTTTCA 
GTGGATGAAT CCAGGCCTGA ACTTATTTTT AGACTAGCTG TGGGAACCTT GTCCATCTCT 
GTGCTTCACA TCGATCCTTT ATCACCACCT GAAACATCAC TCAACCTCAA TCCATTGACA 
CCTTTGGCGA TAGCTTTCTT TACCTGCATA GAAAATATTG ACCCAGCAAG ATTTTCAGCA 
GATGATTTTA AGTCTCTCCG AGCAGTATTT GCGGAAGCTT GCAAACACGA TCACCTTCGA 
TTTATAGGTA CTGGCATCAA AGTGTCCTAT GAACAAAGAC AAAGATCAGC TTCCCGATAT 
TTTAGTACTG ACATGTCCAT CGGGCAAATG GAACTTTTGG AGTGCCTATT TCCCACGGAT 
TTTCATTCTG TTCCTCCTCA CTACACAGAG CTTTTAATGT TCCATTCCAA AGAACGAACT 
GATTCCCATT CACCTGTATG TCTTCAGCTT CATTATAAGC ATTCTGAGAA TAAAGGACCC 
CAGGGCAATC AAGCAAGACT TAGTTCTGTT CCTCAGAAGG CAGAATTACA AATTAAGTTG 
AATCCTGTGT TTTGTGAGCT GGATATCAGT ATTGTGGACA GGTTAAATTC CTTGCTCCAA 
CCACAAAAGC TTACCACAGT AGAGATGATG GCATCTCACA TGTATACTTC ATACAATAAA 
CATATTAGTC TGCACAAGGC TTTCACTGAA GTATTTCTGG ATGATTCACA TAATCCTGCA 
AATTGTCGGA TATCCGTACA AGTTGCCACA CCAGCTTTAA ACCTTTCTGT TCGCTTCCCA 
ATACCTGATC TTCGGTCAGA TCAAGAAAGA GGACCATGGT TTAAGAAGTC ACTTCAGAAG 
GAGATGCTTC ATTTAGCATT CACAGACCTA GAATTTAAGA CTGAATTTAT AGGAGGATCA 
ACCCCAGAGC AAATTAAATT GGAACTTACC TTTAGAGAAC TAGTTGGATC ATTCCAGGAA 
GATAGAGGAG AACCATCTAT TAAGTTTTTC CATGTGTCTA GTGGAGTAGA TGGAGATACA 
GCATCATCCG ATGACTTTGA CTGGCCACGA ATTGTACTCA AATTAAATCC GCCAGCTGTG 
CATTCCATTT TGGAGCGAAT AGCTGCGGAG GAAGAAGAGA GTGATGGCCG CTACCAGGAA 
GAGGAGGAAG GGGGTGCTCA TTCCCTGAAG GATGTTTGTG ATCTGCGAAG ACCGGCCCCA 
TCTCCCTTCT CCTCTCGGAG AGTCATGTTT GAAAATGAGC AGATGGTGAT GCCTGGAGAC 
CCTGTAGAAA TGACAGAGTT TCAGGATAAA GCAATCAGCA ATTCTCACTA TGTGCTGGAA 
CTTACGTTAC CCAACATTCA TATAACACTA CCTAATAAAA GCTTTTATGA GAAGCTTTAT 
AATAGGATCT TTAATGACTT GCTGCTGTGG GAACCAACAG CTCCTTCACC AGTGGAGACA 
TTTGAAAATA TTTCTTATGG TCTTGGTCTT TCTGTAGCCA GTCAGCTGAT TAACACCTTC 
AACAAAGAGA GTTTTAGTCC ATTTAAATCC ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CGTTGCAGTA TTTTTCTACA GTGGATCCCA ACTATCGTTC CCGTAGGAAA 
AAAAAATTAG ACTCTCAGAA CAAGAACTCA CAGAGTTTTC TCTCTGTTCT TCTGAGTATC 
AATCATGGAT TAATGGCAGT GTTTACAGAT GTCAGGCAAG ATAATGGAGA GCTGTTGGAA 
AATAAGCATG GCGAGTTCTG GTTAGAGTTC AACAGTGGTT CCTTGTTTTG TGTGACCAAG 
TATGAAGGCT GTGATGACAA GCACTACATC TGCCTTCATG CCAGCAGTCT CAGTCTATAC 
CACAAAGGCG TAGTAGATGG AGTGATTCTT CCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACACT GGTTGGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTAGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTCT CTGTTGCAGT TAAAATACTT 
TCTGATAAAT CAGAGTCCAA TACAAAGGAA TTTCTCATTG CTGTGGGGTT GAAAGGAGCC 
ACACTCCAGC ACAGAATACT TCCTTCTGGG CTTAGCTGGC ACGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCGTT TACAACTTTT 
CATGTTCATC TCTGGAGCTG CGCACTTGAT TACAGGCCTC TTTATTTGCC GATCCGATCT 
CTTCTCACTG TGGAAACCTT CAGCGTTTCA AGTAGCGTGG CCTTGGACAA ATCCTCGTCT 
ACTCTCAGGA TAATCTTGGA TGAAGCTGCT TTACATCTGT CTGACAAGTG TAATACTGTC 
ACTATTAATT TGAGTAGAGA TTACGTTCGT GTGATGGATA TGGGGCTTTT GGAATTAACC 
ATAACTGCAA TGAAATCTGA TTCTGATGGA GAGCAAACTG AGCCACGCTT CGAGCTACAC 
TGTTCTAGTG ACATTGTCCA TATCCGAACG TGCTCAGACT CTTGTGCTGC GTTAATGAAC 
CTCATTCAAT ACATTGCAAG CTATGGGGAC TTACATGCAG CGAGCAAGGT AGAGATGAAG 
CCTGGAGCCC CTCACCGAAA GATGAAGGTA GAGCCAGCAG CGTCGTTCAT TAGAACTAAC 
TCACCCCTGA CACCTTGCAG CCACGTAGAT TCCAGTGGTC GTTCATCCTC ACGAGGTCCA 
GTCCTTCCTG AAGCCGACCA GCAGATGTTA CGTGATTTGA TGAGTGACGC TATGGAAGAG 
GTTGATCTGC AACAGACTGC TACGTCTACG CAAGCCCAGG CTAACGGTGT TTTGGATGAA 
AAATCTCAAA TTCAGGAGCC TTGTTGTTCA GACCTCTTCC TGTTTCCAGA TGAGAGTGGG 
AATGTGTCCC AGGAGCCTGG CCCTCCTTAT ACCTCATTCA CACCCCACTC GATTAGTGAT 
GCAATGGCAG GTGTGCCTAC TGAGAATGAT GACTTTTGCA TTCTTTTTGC ACCAAGAGCA 
AACAGTCAGG ATAAGGAAGA AGAGCCCATT GTAAAAGTAA TGGTTGATGA TGCAATTGTA 
ATAAGAGAGA ATTACTTTAG TCAGCCTGTT AAGAAGACCG ATACAAGCAA AGCCCCATTG 
CATTTTCCGG TCCCTGTCGT CCGCTATGTT GTTAAAGAAG TCTCTCTTGT TTGGCATCTT 
TATGGAGGAA AGGATTTTGG AACAGCCCCT CCTACTTCTC CAGCTAAAAG TTACATCAGT 
CCTCACAGCT CACCTTCTCA CACACCCACA AGGCATGGAC GCAACATGGT ATGTGGGGGA 
AAAGGAAGAA ACCATGACTT TTTAATGGAA ATACAATTAA GCAAGGTGAA GTTTCAGCAC 
GAGGTCTACC CGCCTTGCAA GCCAGAGTGT GATTCTGGCC TCATAGAGCA CCCAGTCTCC 
CGGCAGGTGT TCATTGTGCA GGATCTTGAA ATTCGAGATC GTCTGGCAAC ATCACAAATG 
AATAAGTTTT TGTACCTGTA TTGTAGTAAA GAAATGCCTC GAAAAGCTCA TTCCAACATG 
CTGACAGTGA AAGCATTACA TGTGCGACCG GAGTCTGGCA GGTCACCACA GGAATGCTGC 
TTGAGAGTAT CCCTAATGCC ACTTCGCCTC AATATTGACC AGGATGCCTT GTTCTTCCTG 
AAGGATTTCT TCACAAGTCT TTCTACGGAA GTAGAGCTTC TAATGGCTCC AGATCCAGAA 
GTTAAAAAGT CTCCTGGAGC TGATGTTACC TGCAGTTTGC CAAGGCACTT AAGCGCCTCC 
AAAGAGCCAA ATCTAGTTCT GTCTTTCCCT GGGCCAAAGC AGCCTTCCCA AGATGATAGT 
GCCAATTCAG CGGAAGTGGT TAATGGGGAA GAAGAGAAGG ACTTCTCAGC TGAAGAAACA 
TCATTTAGTG ATCAGCCCGT GTTTTTTAGA GAATTTAGAT TCACATCAGA AGTACCTATT 
CGACTTGATT ATCATGGCAA ACACGTATCA ATGGATCAGG GTACACTAGC TGGGATTCTG 
ATTGGTCTGG CCCAGTTAAA CTGTTCAGAA CTGAAGCTAA AGAGGCTTTT CTACCGGCAT 
GGCTTGCTAG GTGTTGACAA ATTGTTTTCA TATGCAATCA CCGAATGGCT TACTGACATT 
AAGAAGAATC AGCTACCAGG AATCCTGGGA GGTGTGGGGC CTATGCATTC ACTAGTACAA 
TTAGTGCAAG GCCTAAAGGA CTTGGTCTGG TTACCGATAG AGCAATACCG GAAGGACGGT 
CGTATTGTCA GAGGGTTCCA GAGAGGCGCT GCCTCCTTTG GCACCTCAAC AGCGATGGCT 
GCTCTTGAGC TCACAAACAG AATGGTTCAG ACTATACAGG CAGCAGCAGA GACTGCTTAT 
GATATGGTAT CTCCTGGGAC CCTTTCTATT GAGCCCAAGA AGATCAAAAG GTTTCCTCAT 
CACCGCTTAG CCCACCAGCC AGTGGACCTG CGAGAAGGTG TGGCCAAGGC CTACAGTGTT 
GTGAAAGAGG GACTTACAGA CACAGCTCAG ACGATTTATG AAACTGCAGC ACGAGAACAT 
GAGAGCAGAG GCGTCACTGG TGCTGTGGGC GAGGTCCTGC GCCAGATTCC TCCCGCAGTG 
GTGAAACCTC TGATTGTGGC CACAGAAGCA ACATCAAATG TATTAGGTGG CATGAGAAAC 
CAGATTAGAC CAGATGTTCG GCAAGACGAG TCACAGAAAT GGCGCCATGG GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971622.1
CDS	139..5358
Translation	MQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_014116147.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116147.1

ORF Insert Sequence:

ATGCAGCAGG AAGACGAATA TCGAATTCAG ATGGAATTAA ACCGGTATTA TTTGAGAAAA 
GATTCCCTTT CGGTGGGTGT GTCTTCAGAG CAAAGTTTTT ATGAGACAGA ATCTGCTCGT 
ACACCTTCTA GCCGTGAAGA AGAAGTTTTC TTCTCCATGG CTGATATGGA CATGTCTCAT 
AGTCTCTCTT CTCTTCCACC TCTGGGAGAC CCTCCAAATA TGGACCTTGA GTTATCATTA 
ACTAGTACAT ACACAAATAC TCCAGCAGGA TCTCCATTAA GTGCTACTGT GCTTCAGCCA 
ACGTGGGGAG ATTTCCTTGA TCATCATAAA GAACAACCAG TAAGAGGGTC CACCTTGCCA 
TCCAACCTAG TTCACCCACC AAATTTACAG AAGACTTCTC TCCCATCTAG ATCTGTTTCA 
GTGGATGAAT CCAGGCCTGA ACTTATTTTT AGACTAGCTG TGGGAACCTT GTCCATCTCT 
GTGCTTCACA TCGATCCTTT ATCACCACCT GAAACATCAC TCAACCTCAA TCCATTGACA 
CCTTTGGCGA TAGCTTTCTT TACCTGCATA GAAAATATTG ACCCAGCAAG ATTTTCAGCA 
GATGATTTTA AGTCTCTCCG AGCAGTATTT GCGGAAGCTT GCAAACACGA TCACCTTCGA 
TTTATAGGTA CTGGCATCAA AGTGTCCTAT GAACAAAGAC AAAGATCAGC TTCCCGATAT 
TTTAGTACTG ACATGTCCAT CGGGCAAATG GAACTTTTGG AGTGCCTATT TCCCACGGAT 
TTTCATTCTG TTCCTCCTCA CTACACAGAG CTTTTAATGT TCCATTCCAA AGAACGAACT 
GATTCCCATT CACCTGTATG TCTTCAGCTT CATTATAAGC ATTCTGAGAA TAAAGGACCC 
CAGGGCAATC AAGCAAGACT TAGTTCTGTT CCTCAGAAGG CAGAATTACA AATTAAGTTG 
AATCCTGTGT TTTGTGAGCT GGATATCAGT ATTGTGGACA GGTTAAATTC CTTGCTCCAA 
CCACAAAAGC TTACCACAGT AGAGATGATG GCATCTCACA TGTATACTTC ATACAATAAA 
CATATTAGTC TGCACAAGGC TTTCACTGAA GTATTTCTGG ATGATTCACA TAATCCTGCA 
AATTGTCGGA TATCCGTACA AGTTGCCACA CCAGCTTTAA ACCTTTCTGT TCGCTTCCCA 
ATACCTGATC TTCGGTCAGA TCAAGAAAGA GGACCATGGT TTAAGAAGTC ACTTCAGAAG 
GAGATGCTTC ATTTAGCATT CACAGACCTA GAATTTAAGA CTGAATTTAT AGGAGGATCA 
ACCCCAGAGC AAATTAAATT GGAACTTACC TTTAGAGAAC TAGTTGGATC ATTCCAGGAA 
GATAGAGGAG AACCATCTAT TAAGTTTTTC CATGTGTCTA GTGGAGTAGA TGGAGATACA 
GCATCATCCG ATGACTTTGA CTGGCCACGA ATTGTACTCA AATTAAATCC GCCAGCTGTG 
CATTCCATTT TGGAGCGAAT AGCTGCGGAG GAAGAAGAGA GTGATGGCCG CTACCAGGAA 
GAGGAGGAAG GGGGTGCTCA TTCCCTGAAG GATGTTTGTG ATCTGCGAAG ACCGGCCCCA 
TCTCCCTTCT CCTCTCGGAG AGTCATGTTT GAAAATGAGC AGATGGTGAT GCCTGGAGAC 
CCTGTAGAAA TGACAGAGTT TCAGGATAAA GCAATCAGCA ATTCTCACTA TGTGCTGGAA 
CTTACGTTAC CCAACATTCA TATAACACTA CCTAATAAAA GCTTTTATGA GAAGCTTTAT 
AATAGGATCT TTAATGACTT GCTGCTGTGG GAACCAACAG CTCCTTCACC AGTGGAGACA 
TTTGAAAATA TTTCTTATGG TCTTGGTCTT TCTGTAGCCA GTCAGCTGAT TAACACCTTC 
AACAAAGAGA GTTTTAGTCC ATTTAAATCC ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CGTTGCAGTA TTTTTCTACA GTGGATCCCA ACTATCGTTC CCGTAGGAAA 
AAAAAATTAG ACTCTCAGAA CAAGAACTCA CAGAGTTTTC TCTCTGTTCT TCTGAGTATC 
AATCATGGAT TAATGGCAGT GTTTACAGAT GTCAGGCAAG ATAATGGAGA GCTGTTGGAA 
AATAAGCATG GCGAGTTCTG GTTAGAGTTC AACAGTGGTT CCTTGTTTTG TGTGACCAAG 
TATGAAGGCT GTGATGACAA GCACTACATC TGCCTTCATG CCAGCAGTCT CAGTCTATAC 
CACAAAGGCG TAGTAGATGG AGTGATTCTT CCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACACT GGTTGGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTAGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTCT CTGTTGCAGT TAAAATACTT 
TCTGATAAAT CAGAGTCCAA TACAAAGGAA TTTCTCATTG CTGTGGGGTT GAAAGGAGCC 
ACACTCCAGC ACAGAATACT TCCTTCTGGG CTTAGCTGGC ACGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCGTT TACAACTTTT 
CATGTTCATC TCTGGAGCTG CGCACTTGAT TACAGGCCTC TTTATTTGCC GATCCGATCT 
CTTCTCACTG TGGAAACCTT CAGCGTTTCA AGTAGCGTGG CCTTGGACAA ATCCTCGTCT 
ACTCTCAGGA TAATCTTGGA TGAAGCTGCT TTACATCTGT CTGACAAGTG TAATACTGTC 
ACTATTAATT TGAGTAGAGA TTACGTTCGT GTGATGGATA TGGGGCTTTT GGAATTAACC 
ATAACTGCAA TGAAATCTGA TTCTGATGGA GAGCAAACTG AGCCACGCTT CGAGCTACAC 
TGTTCTAGTG ACATTGTCCA TATCCGAACG TGCTCAGACT CTTGTGCTGC GTTAATGAAC 
CTCATTCAAT ACATTGCAAG CTATGGGGAC TTACATGCAG CGAGCAAGGT AGAGATGAAG 
CCTGGAGCCC CTCACCGAAA GATGAAGGTA GAGCCAGCAG CGTCGTTCAT TAGAACTAAC 
TCACCCCTGA CACCTTGCAG CCACGTAGAT TCCAGTGGTC GTTCATCCTC ACGAGGTCCA 
GTCCTTCCTG AAGCCGACCA GCAGATGTTA CGTGATTTGA TGAGTGACGC TATGGAAGAG 
GTTGATCTGC AACAGACTGC TACGTCTACG CAAGCCCAGG CTAACGGTGT TTTGGATGAA 
AAATCTCAAA TTCAGGAGCC TTGTTGTTCA GACCTCTTCC TGTTTCCAGA TGAGAGTGGG 
AATGTGTCCC AGGAGCCTGG CCCTCCTTAT ACCTCATTCA CACCCCACTC GATTAGTGAT 
GCAATGGCAG GTGTGCCTAC TGAGAATGAT GACTTTTGCA TTCTTTTTGC ACCAAGAGCA 
AACAGTCAGG ATAAGGAAGA AGAGCCCATT GTAAAAGTAA TGGTTGATGA TGCAATTGTA 
ATAAGAGAGA ATTACTTTAG TCAGCCTGTT AAGAAGACCG ATACAAGCAA AGCCCCATTG 
CATTTTCCGG TCCCTGTCGT CCGCTATGTT GTTAAAGAAG TCTCTCTTGT TTGGCATCTT 
TATGGAGGAA AGGATTTTGG AACAGCCCCT CCTACTTCTC CAGCTAAAAG TTACATCAGT 
CCTCACAGCT CACCTTCTCA CACACCCACA AGGCATGGAC GCAACATGGT ATGTGGGGGA 
AAAGGAAGAA ACCATGACTT TTTAATGGAA ATACAATTAA GCAAGGTGAA GTTTCAGCAC 
GAGGTCTACC CGCCTTGCAA GCCAGAGTGT GATTCTGGCC TCATAGAGCA CCCAGTCTCC 
CGGCAGGTGT TCATTGTGCA GGATCTTGAA ATTCGAGATC GTCTGGCAAC ATCACAAATG 
AATAAGTTTT TGTACCTGTA TTGTAGTAAA GAAATGCCTC GAAAAGCTCA TTCCAACATG 
CTGACAGTGA AAGCATTACA TGTGCGACCG GAGTCTGGCA GGTCACCACA GGAATGCTGC 
TTGAGAGTAT CCCTAATGCC ACTTCGCCTC AATATTGACC AGGATGCCTT GTTCTTCCTG 
AAGGATTTCT TCACAAGTCT TTCTACGGAA GTAGAGCTTC TAATGGCTCC AGATCCAGAA 
GTTAAAAAGT CTCCTGGAGC TGATGTTACC TGCAGTTTGC CAAGGCACTT AAGCGCCTCC 
AAAGAGCCAA ATCTAGTTCT GTCTTTCCCT GGGCCAAAGC AGCCTTCCCA AGATGATAGT 
GCCAATTCAG CGGAAGTGGT TAATGGGGAA GAAGAGAAGG ACTTCTCAGC TGAAGAAACA 
TCATTTAGTG ATCAGCCCGT GTTTTTTAGA GAATTTAGAT TCACATCAGA AGTACCTATT 
CGACTTGATT ATCATGGCAA ACACGTATCA ATGGATCAGG GTACACTAGC TGGGATTCTG 
ATTGGTCTGG CCCAGTTAAA CTGTTCAGAA CTGAAGCTAA AGAGGCTTTT CTACCGGCAT 
GGCTTGCTAG GTGTTGACAA ATTGTTTTCA TATGCAATCA CCGAATGGCT TACTGACATT 
AAGAAGAATC AGCTACCAGG AATCCTGGGA GGTGTGGGGC CTATGCATTC ACTAGTACAA 
TTAGTGCAAG GCCTAAAGGA CTTGGTCTGG TTACCGATAG AGCAATACCG GAAGGACGGT 
CGTATTGTCA GAGGGTTCCA GAGAGGCGCT GCCTCCTTTG GCACCTCAAC AGCGATGGCT 
GCTCTTGAGC TCACAAACAG AATGGTTCAG ACTATACAGG CAGCAGCAGA GACTGCTTAT 
GATATGGTAT CTCCTGGGAC CCTTTCTATT GAGCCCAAGA AGATCAAAAG GTTTCCTCAT 
CACCGCTTAG CCCACCAGCC AGTGGACCTG CGAGAAGGTG TGGCCAAGGC CTACAGTGTT 
GTGAAAGAGG GACTTACAGA CACAGCTCAG ACGATTTATG AAACTGCAGC ACGAGAACAT 
GAGAGCAGAG GCGTCACTGG TGCTGTGGGC GAGGTCCTGC GCCAGATTCC TCCCGCAGTG 
GTGAAACCTC TGATTGTGGC CACAGAAGCA ACATCAAATG TATTAGGTGG CATGAGAAAC 
CAGATTAGAC CAGATGTTCG GCAAGACGAG TCACAGAAAT GGCGCCATGG GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116147.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971622.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X8, mRNA.

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CloneID	OCe38048
Clone ID Related Accession (Same CDS sequence)	XM_014116147.1 , XM_014116147.2
Accession Version	XM_014116147.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5220bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116147.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCAGG AAGACGAATA TCGAATTCAG ATGGAATTAA ACCGGTATTA TTTGAGAAAA 
GATTCCCTTT CGGTGGGTGT GTCTTCAGAG CAAAGTTTTT ATGAGACAGA ATCTGCTCGT 
ACACCTTCTA GCCGTGAAGA AGAAGTTTTC TTCTCCATGG CTGATATGGA CATGTCTCAT 
AGTCTCTCTT CTCTTCCACC TCTGGGAGAC CCTCCAAATA TGGACCTTGA GTTATCATTA 
ACTAGTACAT ACACAAATAC TCCAGCAGGA TCTCCATTAA GTGCTACTGT GCTTCAGCCA 
ACGTGGGGAG ATTTCCTTGA TCATCATAAA GAACAACCAG TAAGAGGGTC CACCTTGCCA 
TCCAACCTAG TTCACCCACC AAATTTACAG AAGACTTCTC TCCCATCTAG ATCTGTTTCA 
GTGGATGAAT CCAGGCCTGA ACTTATTTTT AGACTAGCTG TGGGAACCTT GTCCATCTCT 
GTGCTTCACA TCGATCCTTT ATCACCACCT GAAACATCAC TCAACCTCAA TCCATTGACA 
CCTTTGGCGA TAGCTTTCTT TACCTGCATA GAAAATATTG ACCCAGCAAG ATTTTCAGCA 
GATGATTTTA AGTCTCTCCG AGCAGTATTT GCGGAAGCTT GCAAACACGA TCACCTTCGA 
TTTATAGGTA CTGGCATCAA AGTGTCCTAT GAACAAAGAC AAAGATCAGC TTCCCGATAT 
TTTAGTACTG ACATGTCCAT CGGGCAAATG GAACTTTTGG AGTGCCTATT TCCCACGGAT 
TTTCATTCTG TTCCTCCTCA CTACACAGAG CTTTTAATGT TCCATTCCAA AGAACGAACT 
GATTCCCATT CACCTGTATG TCTTCAGCTT CATTATAAGC ATTCTGAGAA TAAAGGACCC 
CAGGGCAATC AAGCAAGACT TAGTTCTGTT CCTCAGAAGG CAGAATTACA AATTAAGTTG 
AATCCTGTGT TTTGTGAGCT GGATATCAGT ATTGTGGACA GGTTAAATTC CTTGCTCCAA 
CCACAAAAGC TTACCACAGT AGAGATGATG GCATCTCACA TGTATACTTC ATACAATAAA 
CATATTAGTC TGCACAAGGC TTTCACTGAA GTATTTCTGG ATGATTCACA TAATCCTGCA 
AATTGTCGGA TATCCGTACA AGTTGCCACA CCAGCTTTAA ACCTTTCTGT TCGCTTCCCA 
ATACCTGATC TTCGGTCAGA TCAAGAAAGA GGACCATGGT TTAAGAAGTC ACTTCAGAAG 
GAGATGCTTC ATTTAGCATT CACAGACCTA GAATTTAAGA CTGAATTTAT AGGAGGATCA 
ACCCCAGAGC AAATTAAATT GGAACTTACC TTTAGAGAAC TAGTTGGATC ATTCCAGGAA 
GATAGAGGAG AACCATCTAT TAAGTTTTTC CATGTGTCTA GTGGAGTAGA TGGAGATACA 
GCATCATCCG ATGACTTTGA CTGGCCACGA ATTGTACTCA AATTAAATCC GCCAGCTGTG 
CATTCCATTT TGGAGCGAAT AGCTGCGGAG GAAGAAGAGA GTGATGGCCG CTACCAGGAA 
GAGGAGGAAG GGGGTGCTCA TTCCCTGAAG GATGTTTGTG ATCTGCGAAG ACCGGCCCCA 
TCTCCCTTCT CCTCTCGGAG AGTCATGTTT GAAAATGAGC AGATGGTGAT GCCTGGAGAC 
CCTGTAGAAA TGACAGAGTT TCAGGATAAA GCAATCAGCA ATTCTCACTA TGTGCTGGAA 
CTTACGTTAC CCAACATTCA TATAACACTA CCTAATAAAA GCTTTTATGA GAAGCTTTAT 
AATAGGATCT TTAATGACTT GCTGCTGTGG GAACCAACAG CTCCTTCACC AGTGGAGACA 
TTTGAAAATA TTTCTTATGG TCTTGGTCTT TCTGTAGCCA GTCAGCTGAT TAACACCTTC 
AACAAAGAGA GTTTTAGTCC ATTTAAATCC ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CGTTGCAGTA TTTTTCTACA GTGGATCCCA ACTATCGTTC CCGTAGGAAA 
AAAAAATTAG ACTCTCAGAA CAAGAACTCA CAGAGTTTTC TCTCTGTTCT TCTGAGTATC 
AATCATGGAT TAATGGCAGT GTTTACAGAT GTCAGGCAAG ATAATGGAGA GCTGTTGGAA 
AATAAGCATG GCGAGTTCTG GTTAGAGTTC AACAGTGGTT CCTTGTTTTG TGTGACCAAG 
TATGAAGGCT GTGATGACAA GCACTACATC TGCCTTCATG CCAGCAGTCT CAGTCTATAC 
CACAAAGGCG TAGTAGATGG AGTGATTCTT CCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACACT GGTTGGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTAGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTCT CTGTTGCAGT TAAAATACTT 
TCTGATAAAT CAGAGTCCAA TACAAAGGAA TTTCTCATTG CTGTGGGGTT GAAAGGAGCC 
ACACTCCAGC ACAGAATACT TCCTTCTGGG CTTAGCTGGC ACGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCGTT TACAACTTTT 
CATGTTCATC TCTGGAGCTG CGCACTTGAT TACAGGCCTC TTTATTTGCC GATCCGATCT 
CTTCTCACTG TGGAAACCTT CAGCGTTTCA AGTAGCGTGG CCTTGGACAA ATCCTCGTCT 
ACTCTCAGGA TAATCTTGGA TGAAGCTGCT TTACATCTGT CTGACAAGTG TAATACTGTC 
ACTATTAATT TGAGTAGAGA TTACGTTCGT GTGATGGATA TGGGGCTTTT GGAATTAACC 
ATAACTGCAA TGAAATCTGA TTCTGATGGA GAGCAAACTG AGCCACGCTT CGAGCTACAC 
TGTTCTAGTG ACATTGTCCA TATCCGAACG TGCTCAGACT CTTGTGCTGC GTTAATGAAC 
CTCATTCAAT ACATTGCAAG CTATGGGGAC TTACATGCAG CGAGCAAGGT AGAGATGAAG 
CCTGGAGCCC CTCACCGAAA GATGAAGGTA GAGCCAGCAG CGTCGTTCAT TAGAACTAAC 
TCACCCCTGA CACCTTGCAG CCACGTAGAT TCCAGTGGTC GTTCATCCTC ACGAGGTCCA 
GTCCTTCCTG AAGCCGACCA GCAGATGTTA CGTGATTTGA TGAGTGACGC TATGGAAGAG 
GTTGATCTGC AACAGACTGC TACGTCTACG CAAGCCCAGG CTAACGGTGT TTTGGATGAA 
AAATCTCAAA TTCAGGAGCC TTGTTGTTCA GACCTCTTCC TGTTTCCAGA TGAGAGTGGG 
AATGTGTCCC AGGAGCCTGG CCCTCCTTAT ACCTCATTCA CACCCCACTC GATTAGTGAT 
GCAATGGCAG GTGTGCCTAC TGAGAATGAT GACTTTTGCA TTCTTTTTGC ACCAAGAGCA 
AACAGTCAGG ATAAGGAAGA AGAGCCCATT GTAAAAGTAA TGGTTGATGA TGCAATTGTA 
ATAAGAGAGA ATTACTTTAG TCAGCCTGTT AAGAAGACCG ATACAAGCAA AGCCCCATTG 
CATTTTCCGG TCCCTGTCGT CCGCTATGTT GTTAAAGAAG TCTCTCTTGT TTGGCATCTT 
TATGGAGGAA AGGATTTTGG AACAGCCCCT CCTACTTCTC CAGCTAAAAG TTACATCAGT 
CCTCACAGCT CACCTTCTCA CACACCCACA AGGCATGGAC GCAACATGGT ATGTGGGGGA 
AAAGGAAGAA ACCATGACTT TTTAATGGAA ATACAATTAA GCAAGGTGAA GTTTCAGCAC 
GAGGTCTACC CGCCTTGCAA GCCAGAGTGT GATTCTGGCC TCATAGAGCA CCCAGTCTCC 
CGGCAGGTGT TCATTGTGCA GGATCTTGAA ATTCGAGATC GTCTGGCAAC ATCACAAATG 
AATAAGTTTT TGTACCTGTA TTGTAGTAAA GAAATGCCTC GAAAAGCTCA TTCCAACATG 
CTGACAGTGA AAGCATTACA TGTGCGACCG GAGTCTGGCA GGTCACCACA GGAATGCTGC 
TTGAGAGTAT CCCTAATGCC ACTTCGCCTC AATATTGACC AGGATGCCTT GTTCTTCCTG 
AAGGATTTCT TCACAAGTCT TTCTACGGAA GTAGAGCTTC TAATGGCTCC AGATCCAGAA 
GTTAAAAAGT CTCCTGGAGC TGATGTTACC TGCAGTTTGC CAAGGCACTT AAGCGCCTCC 
AAAGAGCCAA ATCTAGTTCT GTCTTTCCCT GGGCCAAAGC AGCCTTCCCA AGATGATAGT 
GCCAATTCAG CGGAAGTGGT TAATGGGGAA GAAGAGAAGG ACTTCTCAGC TGAAGAAACA 
TCATTTAGTG ATCAGCCCGT GTTTTTTAGA GAATTTAGAT TCACATCAGA AGTACCTATT 
CGACTTGATT ATCATGGCAA ACACGTATCA ATGGATCAGG GTACACTAGC TGGGATTCTG 
ATTGGTCTGG CCCAGTTAAA CTGTTCAGAA CTGAAGCTAA AGAGGCTTTT CTACCGGCAT 
GGCTTGCTAG GTGTTGACAA ATTGTTTTCA TATGCAATCA CCGAATGGCT TACTGACATT 
AAGAAGAATC AGCTACCAGG AATCCTGGGA GGTGTGGGGC CTATGCATTC ACTAGTACAA 
TTAGTGCAAG GCCTAAAGGA CTTGGTCTGG TTACCGATAG AGCAATACCG GAAGGACGGT 
CGTATTGTCA GAGGGTTCCA GAGAGGCGCT GCCTCCTTTG GCACCTCAAC AGCGATGGCT 
GCTCTTGAGC TCACAAACAG AATGGTTCAG ACTATACAGG CAGCAGCAGA GACTGCTTAT 
GATATGGTAT CTCCTGGGAC CCTTTCTATT GAGCCCAAGA AGATCAAAAG GTTTCCTCAT 
CACCGCTTAG CCCACCAGCC AGTGGACCTG CGAGAAGGTG TGGCCAAGGC CTACAGTGTT 
GTGAAAGAGG GACTTACAGA CACAGCTCAG ACGATTTATG AAACTGCAGC ACGAGAACAT 
GAGAGCAGAG GCGTCACTGG TGCTGTGGGC GAGGTCCTGC GCCAGATTCC TCCCGCAGTG 
GTGAAACCTC TGATTGTGGC CACAGAAGCA ACATCAAATG TATTAGGTGG CATGAGAAAC 
CAGATTAGAC CAGATGTTCG GCAAGACGAG TCACAGAAAT GGCGCCATGG GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971622.1
CDS	181..5400
Translation	MQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_014116147.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116147.2

ORF Insert Sequence:

ATGCAGCAGG AAGACGAATA TCGAATTCAG ATGGAATTAA ACCGGTATTA TTTGAGAAAA 
GATTCCCTTT CGGTGGGTGT GTCTTCAGAG CAAAGTTTTT ATGAGACAGA ATCTGCTCGT 
ACACCTTCTA GCCGTGAAGA AGAAGTTTTC TTCTCCATGG CTGATATGGA CATGTCTCAT 
AGTCTCTCTT CTCTTCCACC TCTGGGAGAC CCTCCAAATA TGGACCTTGA GTTATCATTA 
ACTAGTACAT ACACAAATAC TCCAGCAGGA TCTCCATTAA GTGCTACTGT GCTTCAGCCA 
ACGTGGGGAG ATTTCCTTGA TCATCATAAA GAACAACCAG TAAGAGGGTC CACCTTGCCA 
TCCAACCTAG TTCACCCACC AAATTTACAG AAGACTTCTC TCCCATCTAG ATCTGTTTCA 
GTGGATGAAT CCAGGCCTGA ACTTATTTTT AGACTAGCTG TGGGAACCTT GTCCATCTCT 
GTGCTTCACA TCGATCCTTT ATCACCACCT GAAACATCAC TCAACCTCAA TCCATTGACA 
CCTTTGGCGA TAGCTTTCTT TACCTGCATA GAAAATATTG ACCCAGCAAG ATTTTCAGCA 
GATGATTTTA AGTCTCTCCG AGCAGTATTT GCGGAAGCTT GCAAACACGA TCACCTTCGA 
TTTATAGGTA CTGGCATCAA AGTGTCCTAT GAACAAAGAC AAAGATCAGC TTCCCGATAT 
TTTAGTACTG ACATGTCCAT CGGGCAAATG GAACTTTTGG AGTGCCTATT TCCCACGGAT 
TTTCATTCTG TTCCTCCTCA CTACACAGAG CTTTTAATGT TCCATTCCAA AGAACGAACT 
GATTCCCATT CACCTGTATG TCTTCAGCTT CATTATAAGC ATTCTGAGAA TAAAGGACCC 
CAGGGCAATC AAGCAAGACT TAGTTCTGTT CCTCAGAAGG CAGAATTACA AATTAAGTTG 
AATCCTGTGT TTTGTGAGCT GGATATCAGT ATTGTGGACA GGTTAAATTC CTTGCTCCAA 
CCACAAAAGC TTACCACAGT AGAGATGATG GCATCTCACA TGTATACTTC ATACAATAAA 
CATATTAGTC TGCACAAGGC TTTCACTGAA GTATTTCTGG ATGATTCACA TAATCCTGCA 
AATTGTCGGA TATCCGTACA AGTTGCCACA CCAGCTTTAA ACCTTTCTGT TCGCTTCCCA 
ATACCTGATC TTCGGTCAGA TCAAGAAAGA GGACCATGGT TTAAGAAGTC ACTTCAGAAG 
GAGATGCTTC ATTTAGCATT CACAGACCTA GAATTTAAGA CTGAATTTAT AGGAGGATCA 
ACCCCAGAGC AAATTAAATT GGAACTTACC TTTAGAGAAC TAGTTGGATC ATTCCAGGAA 
GATAGAGGAG AACCATCTAT TAAGTTTTTC CATGTGTCTA GTGGAGTAGA TGGAGATACA 
GCATCATCCG ATGACTTTGA CTGGCCACGA ATTGTACTCA AATTAAATCC GCCAGCTGTG 
CATTCCATTT TGGAGCGAAT AGCTGCGGAG GAAGAAGAGA GTGATGGCCG CTACCAGGAA 
GAGGAGGAAG GGGGTGCTCA TTCCCTGAAG GATGTTTGTG ATCTGCGAAG ACCGGCCCCA 
TCTCCCTTCT CCTCTCGGAG AGTCATGTTT GAAAATGAGC AGATGGTGAT GCCTGGAGAC 
CCTGTAGAAA TGACAGAGTT TCAGGATAAA GCAATCAGCA ATTCTCACTA TGTGCTGGAA 
CTTACGTTAC CCAACATTCA TATAACACTA CCTAATAAAA GCTTTTATGA GAAGCTTTAT 
AATAGGATCT TTAATGACTT GCTGCTGTGG GAACCAACAG CTCCTTCACC AGTGGAGACA 
TTTGAAAATA TTTCTTATGG TCTTGGTCTT TCTGTAGCCA GTCAGCTGAT TAACACCTTC 
AACAAAGAGA GTTTTAGTCC ATTTAAATCC ACAGTTCACT ATGATGAGGA AAGTGGATCT 
GAGGAGGAGA CGTTGCAGTA TTTTTCTACA GTGGATCCCA ACTATCGTTC CCGTAGGAAA 
AAAAAATTAG ACTCTCAGAA CAAGAACTCA CAGAGTTTTC TCTCTGTTCT TCTGAGTATC 
AATCATGGAT TAATGGCAGT GTTTACAGAT GTCAGGCAAG ATAATGGAGA GCTGTTGGAA 
AATAAGCATG GCGAGTTCTG GTTAGAGTTC AACAGTGGTT CCTTGTTTTG TGTGACCAAG 
TATGAAGGCT GTGATGACAA GCACTACATC TGCCTTCATG CCAGCAGTCT CAGTCTATAC 
CACAAAGGCG TAGTAGATGG AGTGATTCTT CCTACAGAAA CACGACTGCC TAGTTCAACC 
CGCCCACACT GGTTGGAACC TACTATTTAT TCCTCTGAAG AAGATGGCCT CAGTAGAGCT 
TCTTCAGATG GTGTTGGAGG AGACACTTTG AATATGCTCT CTGTTGCAGT TAAAATACTT 
TCTGATAAAT CAGAGTCCAA TACAAAGGAA TTTCTCATTG CTGTGGGGTT GAAAGGAGCC 
ACACTCCAGC ACAGAATACT TCCTTCTGGG CTTAGCTGGC ACGAGCAGAT TTTATACTTC 
TTGAATATTG CTGATGAACC TGTTCTGGGA TATAATCCTC CAACTTCGTT TACAACTTTT 
CATGTTCATC TCTGGAGCTG CGCACTTGAT TACAGGCCTC TTTATTTGCC GATCCGATCT 
CTTCTCACTG TGGAAACCTT CAGCGTTTCA AGTAGCGTGG CCTTGGACAA ATCCTCGTCT 
ACTCTCAGGA TAATCTTGGA TGAAGCTGCT TTACATCTGT CTGACAAGTG TAATACTGTC 
ACTATTAATT TGAGTAGAGA TTACGTTCGT GTGATGGATA TGGGGCTTTT GGAATTAACC 
ATAACTGCAA TGAAATCTGA TTCTGATGGA GAGCAAACTG AGCCACGCTT CGAGCTACAC 
TGTTCTAGTG ACATTGTCCA TATCCGAACG TGCTCAGACT CTTGTGCTGC GTTAATGAAC 
CTCATTCAAT ACATTGCAAG CTATGGGGAC TTACATGCAG CGAGCAAGGT AGAGATGAAG 
CCTGGAGCCC CTCACCGAAA GATGAAGGTA GAGCCAGCAG CGTCGTTCAT TAGAACTAAC 
TCACCCCTGA CACCTTGCAG CCACGTAGAT TCCAGTGGTC GTTCATCCTC ACGAGGTCCA 
GTCCTTCCTG AAGCCGACCA GCAGATGTTA CGTGATTTGA TGAGTGACGC TATGGAAGAG 
GTTGATCTGC AACAGACTGC TACGTCTACG CAAGCCCAGG CTAACGGTGT TTTGGATGAA 
AAATCTCAAA TTCAGGAGCC TTGTTGTTCA GACCTCTTCC TGTTTCCAGA TGAGAGTGGG 
AATGTGTCCC AGGAGCCTGG CCCTCCTTAT ACCTCATTCA CACCCCACTC GATTAGTGAT 
GCAATGGCAG GTGTGCCTAC TGAGAATGAT GACTTTTGCA TTCTTTTTGC ACCAAGAGCA 
AACAGTCAGG ATAAGGAAGA AGAGCCCATT GTAAAAGTAA TGGTTGATGA TGCAATTGTA 
ATAAGAGAGA ATTACTTTAG TCAGCCTGTT AAGAAGACCG ATACAAGCAA AGCCCCATTG 
CATTTTCCGG TCCCTGTCGT CCGCTATGTT GTTAAAGAAG TCTCTCTTGT TTGGCATCTT 
TATGGAGGAA AGGATTTTGG AACAGCCCCT CCTACTTCTC CAGCTAAAAG TTACATCAGT 
CCTCACAGCT CACCTTCTCA CACACCCACA AGGCATGGAC GCAACATGGT ATGTGGGGGA 
AAAGGAAGAA ACCATGACTT TTTAATGGAA ATACAATTAA GCAAGGTGAA GTTTCAGCAC 
GAGGTCTACC CGCCTTGCAA GCCAGAGTGT GATTCTGGCC TCATAGAGCA CCCAGTCTCC 
CGGCAGGTGT TCATTGTGCA GGATCTTGAA ATTCGAGATC GTCTGGCAAC ATCACAAATG 
AATAAGTTTT TGTACCTGTA TTGTAGTAAA GAAATGCCTC GAAAAGCTCA TTCCAACATG 
CTGACAGTGA AAGCATTACA TGTGCGACCG GAGTCTGGCA GGTCACCACA GGAATGCTGC 
TTGAGAGTAT CCCTAATGCC ACTTCGCCTC AATATTGACC AGGATGCCTT GTTCTTCCTG 
AAGGATTTCT TCACAAGTCT TTCTACGGAA GTAGAGCTTC TAATGGCTCC AGATCCAGAA 
GTTAAAAAGT CTCCTGGAGC TGATGTTACC TGCAGTTTGC CAAGGCACTT AAGCGCCTCC 
AAAGAGCCAA ATCTAGTTCT GTCTTTCCCT GGGCCAAAGC AGCCTTCCCA AGATGATAGT 
GCCAATTCAG CGGAAGTGGT TAATGGGGAA GAAGAGAAGG ACTTCTCAGC TGAAGAAACA 
TCATTTAGTG ATCAGCCCGT GTTTTTTAGA GAATTTAGAT TCACATCAGA AGTACCTATT 
CGACTTGATT ATCATGGCAA ACACGTATCA ATGGATCAGG GTACACTAGC TGGGATTCTG 
ATTGGTCTGG CCCAGTTAAA CTGTTCAGAA CTGAAGCTAA AGAGGCTTTT CTACCGGCAT 
GGCTTGCTAG GTGTTGACAA ATTGTTTTCA TATGCAATCA CCGAATGGCT TACTGACATT 
AAGAAGAATC AGCTACCAGG AATCCTGGGA GGTGTGGGGC CTATGCATTC ACTAGTACAA 
TTAGTGCAAG GCCTAAAGGA CTTGGTCTGG TTACCGATAG AGCAATACCG GAAGGACGGT 
CGTATTGTCA GAGGGTTCCA GAGAGGCGCT GCCTCCTTTG GCACCTCAAC AGCGATGGCT 
GCTCTTGAGC TCACAAACAG AATGGTTCAG ACTATACAGG CAGCAGCAGA GACTGCTTAT 
GATATGGTAT CTCCTGGGAC CCTTTCTATT GAGCCCAAGA AGATCAAAAG GTTTCCTCAT 
CACCGCTTAG CCCACCAGCC AGTGGACCTG CGAGAAGGTG TGGCCAAGGC CTACAGTGTT 
GTGAAAGAGG GACTTACAGA CACAGCTCAG ACGATTTATG AAACTGCAGC ACGAGAACAT 
GAGAGCAGAG GCGTCACTGG TGCTGTGGGC GAGGTCCTGC GCCAGATTCC TCCCGCAGTG 
GTGAAACCTC TGATTGTGGC CACAGAAGCA ACATCAAATG TATTAGGTGG CATGAGAAAC 
CAGATTAGAC CAGATGTTCG GCAAGACGAG TCACAGAAAT GGCGCCATGG GGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_022422358.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022278066.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OCe290270
Clone ID Related Accession (Same CDS sequence)	XM_022422358.1
Accession Version	XM_022422358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTCC TCACAGCTCA 
CCTTCTCACA CACCCACAAG GCATGGACGC AACATGGTAT GTGGGGGAAA AGGAAGAAAC 
CATGACTTTT TAATGGAAAT ACAATTAAGC AAGGTGAAGT TTCAGCACGA GGTCTACCCG 
CCTTGCAAGC CAGAGTGTGA TTCTGGCCTC ATAGAGCACC CAGTCTCCCG GCAGGTGTTC 
ATTGTGCAGG ATCTTGAAAT TCGAGATCGT CTGGCAACAT CACAAATGAA TAAGTTTTTG 
TACCTGTATT GTAGTAAAGA AATGCCTCGA AAAGCTCATT CCAACATGCT GACAGTGAAA 
GCATTACATG TGCGACCGGA GTCTGGCAGG TCACCACAGG AATGCTGCTT GAGAGTATCC 
CTAATGCCAC TTCGCCTCAA TATTGACCAG GATGCCTTGT TCTTCCTGAA GGATTTCTTC 
ACAAGTCTTT CTACGGAAGT AGAGCTTCTA ATGGCTCCAG ATCCAGAAGT TAAAAAGTCT 
CCTGGAGCTG ATGTTACCTG CAGTTTGCCA AGGCACTTAA GCGCCTCCAA AGAGCCAAAT 
CTAGTTCTGT CTTTCCCTGG GCCAAAGCAG CCTTCCCAAG ATGATAGTGC CAATTCAGCG 
GAAGTGGTTA ATGGGGAAGA AGAGAAGGAC TTCTCAGCTG AAGAAACATC ATTTAGTGAT 
CAGCCCGTGT TTTTTAGAGA ATTTAGATTC ACATCAGAAG TACCTATTCG ACTTGATTAT 
CATGGCAAAC ACGTATCAAT GGATCAGGGT ACACTAGCTG GGATTCTGAT TGGTCTGGCC 
CAGTTAAACT GTTCAGAACT GAAGCTAAAG AGGCTTTTCT ACCGGCATGG CTTGCTAGGT 
GTTGACAAAT TGTTTTCATA TGCAATCACC GAATGGCTTA CTGACATTAA GAAGAATCAG 
CTACCAGGAA TCCTGGGAGG TGTGGGGCCT ATGCATTCAC TAGTACAATT AGTGCAAGGC 
CTAAAGGACT TGGTCTGGTT ACCGATAGAG CAATACCGGA AGGACGGTCG TATTGTCAGA 
GGGTTCCAGA GAGGCGCTGC CTCCTTTGGC ACCTCAACAG CGATGGCTGC TCTTGAGCTC 
ACAAACAGAA TGGTTCAGAC TATACAGGCA GCAGCAGAGA CTGCTTATGA TATGGTATCT 
CCTGGGACCC TTTCTATTGA GCCCAAGAAG ATCAAAAGGT TTCCTCATCA CCGCTTAGCC 
CACCAGCCAG TGGACCTGCG AGAAGGTGTG GCCAAGGCCT ACAGTGTTGT GAAAGAGGGA 
CTTACAGACA CAGCTCAGAC GATTTATGAA ACTGCAGCAC GAGAACATGA GAGCAGAGGC 
GTCACTGGTG CTGTGGGCGA GGTCCTGCGC CAGATTCCTC CCGCAGTGGT GAAACCTCTG 
ATTGTGGCCA CAGAAGCAAC ATCAAATGTA TTAGGTGGCA TGAGAAACCA GATTAGACCA 
GATGTTCGGC AAGACGAGTC ACAGAAATGG CGCCATGGGG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278066.1
CDS	16..6303
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYIPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_022422358.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022422358.1

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATTCC TCACAGCTCA 
CCTTCTCACA CACCCACAAG GCATGGACGC AACATGGTAT GTGGGGGAAA AGGAAGAAAC 
CATGACTTTT TAATGGAAAT ACAATTAAGC AAGGTGAAGT TTCAGCACGA GGTCTACCCG 
CCTTGCAAGC CAGAGTGTGA TTCTGGCCTC ATAGAGCACC CAGTCTCCCG GCAGGTGTTC 
ATTGTGCAGG ATCTTGAAAT TCGAGATCGT CTGGCAACAT CACAAATGAA TAAGTTTTTG 
TACCTGTATT GTAGTAAAGA AATGCCTCGA AAAGCTCATT CCAACATGCT GACAGTGAAA 
GCATTACATG TGCGACCGGA GTCTGGCAGG TCACCACAGG AATGCTGCTT GAGAGTATCC 
CTAATGCCAC TTCGCCTCAA TATTGACCAG GATGCCTTGT TCTTCCTGAA GGATTTCTTC 
ACAAGTCTTT CTACGGAAGT AGAGCTTCTA ATGGCTCCAG ATCCAGAAGT TAAAAAGTCT 
CCTGGAGCTG ATGTTACCTG CAGTTTGCCA AGGCACTTAA GCGCCTCCAA AGAGCCAAAT 
CTAGTTCTGT CTTTCCCTGG GCCAAAGCAG CCTTCCCAAG ATGATAGTGC CAATTCAGCG 
GAAGTGGTTA ATGGGGAAGA AGAGAAGGAC TTCTCAGCTG AAGAAACATC ATTTAGTGAT 
CAGCCCGTGT TTTTTAGAGA ATTTAGATTC ACATCAGAAG TACCTATTCG ACTTGATTAT 
CATGGCAAAC ACGTATCAAT GGATCAGGGT ACACTAGCTG GGATTCTGAT TGGTCTGGCC 
CAGTTAAACT GTTCAGAACT GAAGCTAAAG AGGCTTTTCT ACCGGCATGG CTTGCTAGGT 
GTTGACAAAT TGTTTTCATA TGCAATCACC GAATGGCTTA CTGACATTAA GAAGAATCAG 
CTACCAGGAA TCCTGGGAGG TGTGGGGCCT ATGCATTCAC TAGTACAATT AGTGCAAGGC 
CTAAAGGACT TGGTCTGGTT ACCGATAGAG CAATACCGGA AGGACGGTCG TATTGTCAGA 
GGGTTCCAGA GAGGCGCTGC CTCCTTTGGC ACCTCAACAG CGATGGCTGC TCTTGAGCTC 
ACAAACAGAA TGGTTCAGAC TATACAGGCA GCAGCAGAGA CTGCTTATGA TATGGTATCT 
CCTGGGACCC TTTCTATTGA GCCCAAGAAG ATCAAAAGGT TTCCTCATCA CCGCTTAGCC 
CACCAGCCAG TGGACCTGCG AGAAGGTGTG GCCAAGGCCT ACAGTGTTGT GAAAGAGGGA 
CTTACAGACA CAGCTCAGAC GATTTATGAA ACTGCAGCAC GAGAACATGA GAGCAGAGGC 
GTCACTGGTG CTGTGGGCGA GGTCCTGCGC CAGATTCCTC CCGCAGTGGT GAAACCTCTG 
ATTGTGGCCA CAGAAGCAAC ATCAAATGTA TTAGGTGGCA TGAGAAACCA GATTAGACCA 
GATGTTCGGC AAGACGAGTC ACAGAAATGG CGCCATGGGG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_022422359.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022278067.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X5, mRNA.

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CloneID	OCe290271
Clone ID Related Accession (Same CDS sequence)	XM_022422359.1
Accession Version	XM_022422359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGATTCCAGT GGTCGTTCAT CCTCACGAGG TCCAGTCCTT 
CCTGAAGCCG ACCAGCAGAT GTTACGTGAT TTGATGAGTG ACGCTATGGA AGAGGTTGAT 
CTGCAACAGA CTGCTACGTC TACGCAAGCC CAGGCTAACG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCTTGTTG TTCAGACCTC TTCCTGTTTC CAGATGAGAG TGGGAATGTG 
TCCCAGGAGC CTGGCCCTCC TTATACCTCA TTCACACCCC ACTCGATTAG TGATGCAATG 
GCAGGTGTGC CTACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAG AGCAAACAGT 
CAGGATAAGG AAGAAGAGCC CATTGTAAAA GTAATGGTTG ATGATGCAAT TGTAATAAGA 
GAGAATTACT TTAGTCAGCC TGTTAAGAAG ACCGATACAA GCAAAGCCCC ATTGCATTTT 
CCGGTCCCTG TCGTCCGCTA TGTTGTTAAA GAAGTCTCTC TTGTTTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAACAGC CCCTCCTACT TCTCCAGCTA AAAGTTACAT TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGCTTGCTA 
GGTGTTGACA AATTGTTTTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAT 
CAGCTACCAG GAATCCTGGG AGGTGTGGGG CCTATGCATT CACTAGTACA ATTAGTGCAA 
GGCCTAAAGG ACTTGGTCTG GTTACCGATA GAGCAATACC GGAAGGACGG TCGTATTGTC 
AGAGGGTTCC AGAGAGGCGC TGCCTCCTTT GGCACCTCAA CAGCGATGGC TGCTCTTGAG 
CTCACAAACA GAATGGTTCA GACTATACAG GCAGCAGCAG AGACTGCTTA TGATATGGTA 
TCTCCTGGGA CCCTTTCTAT TGAGCCCAAG AAGATCAAAA GGTTTCCTCA TCACCGCTTA 
GCCCACCAGC CAGTGGACCT GCGAGAAGGT GTGGCCAAGG CCTACAGTGT TGTGAAAGAG 
GGACTTACAG ACACAGCTCA GACGATTTAT GAAACTGCAG CACGAGAACA TGAGAGCAGA 
GGCGTCACTG GTGCTGTGGG CGAGGTCCTG CGCCAGATTC CTCCCGCAGT GGTGAAACCT 
CTGATTGTGG CCACAGAAGC AACATCAAAT GTATTAGGTG GCATGAGAAA CCAGATTAGA 
CCAGATGTTC GGCAAGACGA GTCACAGAAA TGGCGCCATG GGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278067.1
CDS	16..6246
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYIPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_022422359.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022422359.1

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGATTCCAGT GGTCGTTCAT CCTCACGAGG TCCAGTCCTT 
CCTGAAGCCG ACCAGCAGAT GTTACGTGAT TTGATGAGTG ACGCTATGGA AGAGGTTGAT 
CTGCAACAGA CTGCTACGTC TACGCAAGCC CAGGCTAACG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCTTGTTG TTCAGACCTC TTCCTGTTTC CAGATGAGAG TGGGAATGTG 
TCCCAGGAGC CTGGCCCTCC TTATACCTCA TTCACACCCC ACTCGATTAG TGATGCAATG 
GCAGGTGTGC CTACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAG AGCAAACAGT 
CAGGATAAGG AAGAAGAGCC CATTGTAAAA GTAATGGTTG ATGATGCAAT TGTAATAAGA 
GAGAATTACT TTAGTCAGCC TGTTAAGAAG ACCGATACAA GCAAAGCCCC ATTGCATTTT 
CCGGTCCCTG TCGTCCGCTA TGTTGTTAAA GAAGTCTCTC TTGTTTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAACAGC CCCTCCTACT TCTCCAGCTA AAAGTTACAT TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGCTTGCTA 
GGTGTTGACA AATTGTTTTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAT 
CAGCTACCAG GAATCCTGGG AGGTGTGGGG CCTATGCATT CACTAGTACA ATTAGTGCAA 
GGCCTAAAGG ACTTGGTCTG GTTACCGATA GAGCAATACC GGAAGGACGG TCGTATTGTC 
AGAGGGTTCC AGAGAGGCGC TGCCTCCTTT GGCACCTCAA CAGCGATGGC TGCTCTTGAG 
CTCACAAACA GAATGGTTCA GACTATACAG GCAGCAGCAG AGACTGCTTA TGATATGGTA 
TCTCCTGGGA CCCTTTCTAT TGAGCCCAAG AAGATCAAAA GGTTTCCTCA TCACCGCTTA 
GCCCACCAGC CAGTGGACCT GCGAGAAGGT GTGGCCAAGG CCTACAGTGT TGTGAAAGAG 
GGACTTACAG ACACAGCTCA GACGATTTAT GAAACTGCAG CACGAGAACA TGAGAGCAGA 
GGCGTCACTG GTGCTGTGGG CGAGGTCCTG CGCCAGATTC CTCCCGCAGT GGTGAAACCT 
CTGATTGTGG CCACAGAAGC AACATCAAAT GTATTAGGTG GCATGAGAAA CCAGATTAGA 
CCAGATGTTC GGCAAGACGA GTCACAGAAA TGGCGCCATG GGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116144.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971619.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OCe38045
Clone ID Related Accession (Same CDS sequence)	XM_014116144.1 , XM_014116144.2
Accession Version	XM_014116144.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGCTTGCTA 
GGTGTTGACA AATTGTTTTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAT 
CAGCTACCAG GAATCCTGGG AGGTGTGGGG CCTATGCATT CACTAGTACA ATTAGTGCAA 
GGCCTAAAGG ACTTGGTCTG GTTACCGATA GAGCAATACC GGAAGGACGG TCGTATTGTC 
AGAGGGTTCC AGAGAGGCGC TGCCTCCTTT GGCACCTCAA CAGCGATGGC TGCTCTTGAG 
CTCACAAACA GAATGGTTCA GACTATACAG GCAGCAGCAG AGACTGCTTA TGATATGGTA 
TCTCCTGGGA CCCTTTCTAT TGAGCCCAAG AAGATCAAAA GGTTTCCTCA TCACCGCTTA 
GCCCACCAGC CAGTGGACCT GCGAGAAGGT GTGGCCAAGG CCTACAGTGT TGTGAAAGAG 
GGACTTACAG ACACAGCTCA GACGATTTAT GAAACTGCAG CACGAGAACA TGAGAGCAGA 
GGCGTCACTG GTGCTGTGGG CGAGGTCCTG CGCCAGATTC CTCCCGCAGT GGTGAAACCT 
CTGATTGTGG CCACAGAAGC AACATCAAAT GTATTAGGTG GCATGAGAAA CCAGATTAGA 
CCAGATGTTC GGCAAGACGA GTCACAGAAA TGGCGCCATG GGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971619.1
CDS	2..6292
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_014116144.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116144.1

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGCTTGCTA 
GGTGTTGACA AATTGTTTTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAT 
CAGCTACCAG GAATCCTGGG AGGTGTGGGG CCTATGCATT CACTAGTACA ATTAGTGCAA 
GGCCTAAAGG ACTTGGTCTG GTTACCGATA GAGCAATACC GGAAGGACGG TCGTATTGTC 
AGAGGGTTCC AGAGAGGCGC TGCCTCCTTT GGCACCTCAA CAGCGATGGC TGCTCTTGAG 
CTCACAAACA GAATGGTTCA GACTATACAG GCAGCAGCAG AGACTGCTTA TGATATGGTA 
TCTCCTGGGA CCCTTTCTAT TGAGCCCAAG AAGATCAAAA GGTTTCCTCA TCACCGCTTA 
GCCCACCAGC CAGTGGACCT GCGAGAAGGT GTGGCCAAGG CCTACAGTGT TGTGAAAGAG 
GGACTTACAG ACACAGCTCA GACGATTTAT GAAACTGCAG CACGAGAACA TGAGAGCAGA 
GGCGTCACTG GTGCTGTGGG CGAGGTCCTG CGCCAGATTC CTCCCGCAGT GGTGAAACCT 
CTGATTGTGG CCACAGAAGC AACATCAAAT GTATTAGGTG GCATGAGAAA CCAGATTAGA 
CCAGATGTTC GGCAAGACGA GTCACAGAAA TGGCGCCATG GGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116144.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971619.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X1, mRNA.

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CloneID	OCe38045
Clone ID Related Accession (Same CDS sequence)	XM_014116144.1 , XM_014116144.2
Accession Version	XM_014116144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116144.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGCTTGCTA 
GGTGTTGACA AATTGTTTTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAT 
CAGCTACCAG GAATCCTGGG AGGTGTGGGG CCTATGCATT CACTAGTACA ATTAGTGCAA 
GGCCTAAAGG ACTTGGTCTG GTTACCGATA GAGCAATACC GGAAGGACGG TCGTATTGTC 
AGAGGGTTCC AGAGAGGCGC TGCCTCCTTT GGCACCTCAA CAGCGATGGC TGCTCTTGAG 
CTCACAAACA GAATGGTTCA GACTATACAG GCAGCAGCAG AGACTGCTTA TGATATGGTA 
TCTCCTGGGA CCCTTTCTAT TGAGCCCAAG AAGATCAAAA GGTTTCCTCA TCACCGCTTA 
GCCCACCAGC CAGTGGACCT GCGAGAAGGT GTGGCCAAGG CCTACAGTGT TGTGAAAGAG 
GGACTTACAG ACACAGCTCA GACGATTTAT GAAACTGCAG CACGAGAACA TGAGAGCAGA 
GGCGTCACTG GTGCTGTGGG CGAGGTCCTG CGCCAGATTC CTCCCGCAGT GGTGAAACCT 
CTGATTGTGG CCACAGAAGC AACATCAAAT GTATTAGGTG GCATGAGAAA CCAGATTAGA 
CCAGATGTTC GGCAAGACGA GTCACAGAAA TGGCGCCATG GGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971619.1
CDS	16..6306
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_014116144.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116144.2

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGAGCCAGCA GCGTCGTTCA TTAGAACTAA CTCACCCCTG 
ACACCTTGCA GCCACGTAGA TTCCAGTGGT CGTTCATCCT CACGAGGTCC AGTCCTTCCT 
GAAGCCGACC AGCAGATGTT ACGTGATTTG ATGAGTGACG CTATGGAAGA GGTTGATCTG 
CAACAGACTG CTACGTCTAC GCAAGCCCAG GCTAACGGTG TTTTGGATGA AAAATCTCAA 
ATTCAGGAGC CTTGTTGTTC AGACCTCTTC CTGTTTCCAG ATGAGAGTGG GAATGTGTCC 
CAGGAGCCTG GCCCTCCTTA TACCTCATTC ACACCCCACT CGATTAGTGA TGCAATGGCA 
GGTGTGCCTA CTGAGAATGA TGACTTTTGC ATTCTTTTTG CACCAAGAGC AAACAGTCAG 
GATAAGGAAG AAGAGCCCAT TGTAAAAGTA ATGGTTGATG ATGCAATTGT AATAAGAGAG 
AATTACTTTA GTCAGCCTGT TAAGAAGACC GATACAAGCA AAGCCCCATT GCATTTTCCG 
GTCCCTGTCG TCCGCTATGT TGTTAAAGAA GTCTCTCTTG TTTGGCATCT TTATGGAGGA 
AAGGATTTTG GAACAGCCCC TCCTACTTCT CCAGCTAAAA GTTACATCAG TCCTCACAGC 
TCACCTTCTC ACACACCCAC AAGGCATGGA CGCAACATGG TATGTGGGGG AAAAGGAAGA 
AACCATGACT TTTTAATGGA AATACAATTA AGCAAGGTGA AGTTTCAGCA CGAGGTCTAC 
CCGCCTTGCA AGCCAGAGTG TGATTCTGGC CTCATAGAGC ACCCAGTCTC CCGGCAGGTG 
TTCATTGTGC AGGATCTTGA AATTCGAGAT CGTCTGGCAA CATCACAAAT GAATAAGTTT 
TTGTACCTGT ATTGTAGTAA AGAAATGCCT CGAAAAGCTC ATTCCAACAT GCTGACAGTG 
AAAGCATTAC ATGTGCGACC GGAGTCTGGC AGGTCACCAC AGGAATGCTG CTTGAGAGTA 
TCCCTAATGC CACTTCGCCT CAATATTGAC CAGGATGCCT TGTTCTTCCT GAAGGATTTC 
TTCACAAGTC TTTCTACGGA AGTAGAGCTT CTAATGGCTC CAGATCCAGA AGTTAAAAAG 
TCTCCTGGAG CTGATGTTAC CTGCAGTTTG CCAAGGCACT TAAGCGCCTC CAAAGAGCCA 
AATCTAGTTC TGTCTTTCCC TGGGCCAAAG CAGCCTTCCC AAGATGATAG TGCCAATTCA 
GCGGAAGTGG TTAATGGGGA AGAAGAGAAG GACTTCTCAG CTGAAGAAAC ATCATTTAGT 
GATCAGCCCG TGTTTTTTAG AGAATTTAGA TTCACATCAG AAGTACCTAT TCGACTTGAT 
TATCATGGCA AACACGTATC AATGGATCAG GGTACACTAG CTGGGATTCT GATTGGTCTG 
GCCCAGTTAA ACTGTTCAGA ACTGAAGCTA AAGAGGCTTT TCTACCGGCA TGGCTTGCTA 
GGTGTTGACA AATTGTTTTC ATATGCAATC ACCGAATGGC TTACTGACAT TAAGAAGAAT 
CAGCTACCAG GAATCCTGGG AGGTGTGGGG CCTATGCATT CACTAGTACA ATTAGTGCAA 
GGCCTAAAGG ACTTGGTCTG GTTACCGATA GAGCAATACC GGAAGGACGG TCGTATTGTC 
AGAGGGTTCC AGAGAGGCGC TGCCTCCTTT GGCACCTCAA CAGCGATGGC TGCTCTTGAG 
CTCACAAACA GAATGGTTCA GACTATACAG GCAGCAGCAG AGACTGCTTA TGATATGGTA 
TCTCCTGGGA CCCTTTCTAT TGAGCCCAAG AAGATCAAAA GGTTTCCTCA TCACCGCTTA 
GCCCACCAGC CAGTGGACCT GCGAGAAGGT GTGGCCAAGG CCTACAGTGT TGTGAAAGAG 
GGACTTACAG ACACAGCTCA GACGATTTAT GAAACTGCAG CACGAGAACA TGAGAGCAGA 
GGCGTCACTG GTGCTGTGGG CGAGGTCCTG CGCCAGATTC CTCCCGCAGT GGTGAAACCT 
CTGATTGTGG CCACAGAAGC AACATCAAAT GTATTAGGTG GCATGAGAAA CCAGATTAGA 
CCAGATGTTC GGCAAGACGA GTCACAGAAA TGGCGCCATG GGGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116145.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971620.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OCe38046
Clone ID Related Accession (Same CDS sequence)	XM_014116145.2 , XM_014116145.1
Accession Version	XM_014116145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014116145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGTTTTCTT CTCCATGGCT GATATGGACA TGTCTCATAG TCTCTCTTCT 
CTTCCACCTC TGGGAGACCC TCCAAATATG GACCTTGAGT TATCATTAAC TAGTACATAC 
ACAAATACTC CAGCAGGATC TCCATTAAGT GCTACTGTGC TTCAGCCAAC GTGGGGAGAT 
TTCCTTGATC ATCATAAAGA ACAACCAGTA AGAGGGTCCA CCTTGCCATC CAACCTAGTT 
CACCCACCAA ATTTACAGAA GACTTCTCTC CCATCTAGAT CTGTTTCAGT GGATGAATCC 
AGGCCTGAAC TTATTTTTAG ACTAGCTGTG GGAACCTTGT CCATCTCTGT GCTTCACATC 
GATCCTTTAT CACCACCTGA AACATCACTC AACCTCAATC CATTGACACC TTTGGCGATA 
GCTTTCTTTA CCTGCATAGA AAATATTGAC CCAGCAAGAT TTTCAGCAGA TGATTTTAAG 
TCTCTCCGAG CAGTATTTGC GGAAGCTTGC AAACACGATC ACCTTCGATT TATAGGTACT 
GGCATCAAAG TGTCCTATGA ACAAAGACAA AGATCAGCTT CCCGATATTT TAGTACTGAC 
ATGTCCATCG GGCAAATGGA ACTTTTGGAG TGCCTATTTC CCACGGATTT TCATTCTGTT 
CCTCCTCACT ACACAGAGCT TTTAATGTTC CATTCCAAAG AACGAACTGA TTCCCATTCA 
CCTGTATGTC TTCAGCTTCA TTATAAGCAT TCTGAGAATA AAGGACCCCA GGGCAATCAA 
GCAAGACTTA GTTCTGTTCC TCAGAAGGCA GAATTACAAA TTAAGTTGAA TCCTGTGTTT 
TGTGAGCTGG ATATCAGTAT TGTGGACAGG TTAAATTCCT TGCTCCAACC ACAAAAGCTT 
ACCACAGTAG AGATGATGGC ATCTCACATG TATACTTCAT ACAATAAACA TATTAGTCTG 
CACAAGGCTT TCACTGAAGT ATTTCTGGAT GATTCACATA ATCCTGCAAA TTGTCGGATA 
TCCGTACAAG TTGCCACACC AGCTTTAAAC CTTTCTGTTC GCTTCCCAAT ACCTGATCTT 
CGGTCAGATC AAGAAAGAGG ACCATGGTTT AAGAAGTCAC TTCAGAAGGA GATGCTTCAT 
TTAGCATTCA CAGACCTAGA ATTTAAGACT GAATTTATAG GAGGATCAAC CCCAGAGCAA 
ATTAAATTGG AACTTACCTT TAGAGAACTA GTTGGATCAT TCCAGGAAGA TAGAGGAGAA 
CCATCTATTA AGTTTTTCCA TGTGTCTAGT GGAGTAGATG GAGATACAGC ATCATCCGAT 
GACTTTGACT GGCCACGAAT TGTACTCAAA TTAAATCCGC CAGCTGTGCA TTCCATTTTG 
GAGCGAATAG CTGCGGAGGA AGAAGAGAGT GATGGCCGCT ACCAGGAAGA GGAGGAAGGG 
GGTGCTCATT CCCTGAAGGA TGTTTGTGAT CTGCGAAGAC CGGCCCCATC TCCCTTCTCC 
TCTCGGAGAG TCATGTTTGA AAATGAGCAG ATGGTGATGC CTGGAGACCC TGTAGAAATG 
ACAGAGTTTC AGGATAAAGC AATCAGCAAT TCTCACTATG TGCTGGAACT TACGTTACCC 
AACATTCATA TAACACTACC TAATAAAAGC TTTTATGAGA AGCTTTATAA TAGGATCTTT 
AATGACTTGC TGCTGTGGGA ACCAACAGCT CCTTCACCAG TGGAGACATT TGAAAATATT 
TCTTATGGTC TTGGTCTTTC TGTAGCCAGT CAGCTGATTA ACACCTTCAA CAAAGAGAGT 
TTTAGTCCAT TTAAATCCAC AGTTCACTAT GATGAGGAAA GTGGATCTGA GGAGGAGACG 
TTGCAGTATT TTTCTACAGT GGATCCCAAC TATCGTTCCC GTAGGAAAAA AAAATTAGAC 
TCTCAGAACA AGAACTCACA GAGTTTTCTC TCTGTTCTTC TGAGTATCAA TCATGGATTA 
ATGGCAGTGT TTACAGATGT CAGGCAAGAT AATGGAGAGC TGTTGGAAAA TAAGCATGGC 
GAGTTCTGGT TAGAGTTCAA CAGTGGTTCC TTGTTTTGTG TGACCAAGTA TGAAGGCTGT 
GATGACAAGC ACTACATCTG CCTTCATGCC AGCAGTCTCA GTCTATACCA CAAAGGCGTA 
GTAGATGGAG TGATTCTTCC TACAGAAACA CGACTGCCTA GTTCAACCCG CCCACACTGG 
TTGGAACCTA CTATTTATTC CTCTGAAGAA GATGGCCTCA GTAGAGCTTC TTCAGATGGT 
GTTGGAGGAG ACACTTTGAA TATGCTCTCT GTTGCAGTTA AAATACTTTC TGATAAATCA 
GAGTCCAATA CAAAGGAATT TCTCATTGCT GTGGGGTTGA AAGGAGCCAC ACTCCAGCAC 
AGAATACTTC CTTCTGGGCT TAGCTGGCAC GAGCAGATTT TATACTTCTT GAATATTGCT 
GATGAACCTG TTCTGGGATA TAATCCTCCA ACTTCGTTTA CAACTTTTCA TGTTCATCTC 
TGGAGCTGCG CACTTGATTA CAGGCCTCTT TATTTGCCGA TCCGATCTCT TCTCACTGTG 
GAAACCTTCA GCGTTTCAAG TAGCGTGGCC TTGGACAAAT CCTCGTCTAC TCTCAGGATA 
ATCTTGGATG AAGCTGCTTT ACATCTGTCT GACAAGTGTA ATACTGTCAC TATTAATTTG 
AGTAGAGATT ACGTTCGTGT GATGGATATG GGGCTTTTGG AATTAACCAT AACTGCAATG 
AAATCTGATT CTGATGGAGA GCAAACTGAG CCACGCTTCG AGCTACACTG TTCTAGTGAC 
ATTGTCCATA TCCGAACGTG CTCAGACTCT TGTGCTGCGT TAATGAACCT CATTCAATAC 
ATTGCAAGCT ATGGGGACTT ACATGCAGCG AGCAAGGTAG AGATGAAGCC TGGAGCCCCT 
CACCGAAAGA TGAAGGTAGA GCCAGCAGCG TCGTTCATTA GAACTAACTC ACCCCTGACA 
CCTTGCAGCC ACGTAGATTC CAGTGGTCGT TCATCCTCAC GAGGTCCAGT CCTTCCTGAA 
GCCGACCAGC AGATGTTACG TGATTTGATG AGTGACGCTA TGGAAGAGGT TGATCTGCAA 
CAGACTGCTA CGTCTACGCA AGCCCAGGCT AACGGTGTTT TGGATGAAAA ATCTCAAATT 
CAGGAGCCTT GTTGTTCAGA CCTCTTCCTG TTTCCAGATG AGAGTGGGAA TGTGTCCCAG 
GAGCCTGGCC CTCCTTATAC CTCATTCACA CCCCACTCGA TTAGTGATGC AATGGCAGGT 
GTGCCTACTG AGAATGATGA CTTTTGCATT CTTTTTGCAC CAAGAGCAAA CAGTCAGGAT 
AAGGAAGAAG AGCCCATTGT AAAAGTAATG GTTGATGATG CAATTGTAAT AAGAGAGAAT 
TACTTTAGTC AGCCTGTTAA GAAGACCGAT ACAAGCAAAG CCCCATTGCA TTTTCCGGTC 
CCTGTCGTCC GCTATGTTGT TAAAGAAGTC TCTCTTGTTT GGCATCTTTA TGGAGGAAAG 
GATTTTGGAA CAGCCCCTCC TACTTCTCCA GCTAAAAGTT ACATCAGTCC TCACAGCTCA 
CCTTCTCACA CACCCACAAG GCATGGACGC AACATGGTAT GTGGGGGAAA AGGAAGAAAC 
CATGACTTTT TAATGGAAAT ACAATTAAGC AAGGTGAAGT TTCAGCACGA GGTCTACCCG 
CCTTGCAAGC CAGAGTGTGA TTCTGGCCTC ATAGAGCACC CAGTCTCCCG GCAGGTGTTC 
ATTGTGCAGG ATCTTGAAAT TCGAGATCGT CTGGCAACAT CACAAATGAA TAAGTTTTTG 
TACCTGTATT GTAGTAAAGA AATGCCTCGA AAAGCTCATT CCAACATGCT GACAGTGAAA 
GCATTACATG TGCGACCGGA GTCTGGCAGG TCACCACAGG AATGCTGCTT GAGAGTATCC 
CTAATGCCAC TTCGCCTCAA TATTGACCAG GATGCCTTGT TCTTCCTGAA GGATTTCTTC 
ACAAGTCTTT CTACGGAAGT AGAGCTTCTA ATGGCTCCAG ATCCAGAAGT TAAAAAGTCT 
CCTGGAGCTG ATGTTACCTG CAGTTTGCCA AGGCACTTAA GCGCCTCCAA AGAGCCAAAT 
CTAGTTCTGT CTTTCCCTGG GCCAAAGCAG CCTTCCCAAG ATGATAGTGC CAATTCAGCG 
GAAGTGGTTA ATGGGGAAGA AGAGAAGGAC TTCTCAGCTG AAGAAACATC ATTTAGTGAT 
CAGCCCGTGT TTTTTAGAGA ATTTAGATTC ACATCAGAAG TACCTATTCG ACTTGATTAT 
CATGGCAAAC ACGTATCAAT GGATCAGGGT ACACTAGCTG GGATTCTGAT TGGTCTGGCC 
CAGTTAAACT GTTCAGAACT GAAGCTAAAG AGGCTTTTCT ACCGGCATGG CTTGCTAGGT 
GTTGACAAAT TGTTTTCATA TGCAATCACC GAATGGCTTA CTGACATTAA GAAGAATCAG 
CTACCAGGAA TCCTGGGAGG TGTGGGGCCT ATGCATTCAC TAGTACAATT AGTGCAAGGC 
CTAAAGGACT TGGTCTGGTT ACCGATAGAG CAATACCGGA AGGACGGTCG TATTGTCAGA 
GGGTTCCAGA GAGGCGCTGC CTCCTTTGGC ACCTCAACAG CGATGGCTGC TCTTGAGCTC 
ACAAACAGAA TGGTTCAGAC TATACAGGCA GCAGCAGAGA CTGCTTATGA TATGGTATCT 
CCTGGGACCC TTTCTATTGA GCCCAAGAAG ATCAAAAGGT TTCCTCATCA CCGCTTAGCC 
CACCAGCCAG TGGACCTGCG AGAAGGTGTG GCCAAGGCCT ACAGTGTTGT GAAAGAGGGA 
CTTACAGACA CAGCTCAGAC GATTTATGAA ACTGCAGCAC GAGAACATGA GAGCAGAGGC 
GTCACTGGTG CTGTGGGCGA GGTCCTGCGC CAGATTCCTC CCGCAGTGGT GAAACCTCTG 
ATTGTGGCCA CAGAAGCAAC ATCAAATGTA TTAGGTGGCA TGAGAAACCA GATTAGACCA 
GATGTTCGGC AAGACGAGTC ACAGAAATGG CGCCATGGGG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971620.1
CDS	16..6303
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_014116145.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116145.2

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGTTTTCTT CTCCATGGCT GATATGGACA TGTCTCATAG TCTCTCTTCT 
CTTCCACCTC TGGGAGACCC TCCAAATATG GACCTTGAGT TATCATTAAC TAGTACATAC 
ACAAATACTC CAGCAGGATC TCCATTAAGT GCTACTGTGC TTCAGCCAAC GTGGGGAGAT 
TTCCTTGATC ATCATAAAGA ACAACCAGTA AGAGGGTCCA CCTTGCCATC CAACCTAGTT 
CACCCACCAA ATTTACAGAA GACTTCTCTC CCATCTAGAT CTGTTTCAGT GGATGAATCC 
AGGCCTGAAC TTATTTTTAG ACTAGCTGTG GGAACCTTGT CCATCTCTGT GCTTCACATC 
GATCCTTTAT CACCACCTGA AACATCACTC AACCTCAATC CATTGACACC TTTGGCGATA 
GCTTTCTTTA CCTGCATAGA AAATATTGAC CCAGCAAGAT TTTCAGCAGA TGATTTTAAG 
TCTCTCCGAG CAGTATTTGC GGAAGCTTGC AAACACGATC ACCTTCGATT TATAGGTACT 
GGCATCAAAG TGTCCTATGA ACAAAGACAA AGATCAGCTT CCCGATATTT TAGTACTGAC 
ATGTCCATCG GGCAAATGGA ACTTTTGGAG TGCCTATTTC CCACGGATTT TCATTCTGTT 
CCTCCTCACT ACACAGAGCT TTTAATGTTC CATTCCAAAG AACGAACTGA TTCCCATTCA 
CCTGTATGTC TTCAGCTTCA TTATAAGCAT TCTGAGAATA AAGGACCCCA GGGCAATCAA 
GCAAGACTTA GTTCTGTTCC TCAGAAGGCA GAATTACAAA TTAAGTTGAA TCCTGTGTTT 
TGTGAGCTGG ATATCAGTAT TGTGGACAGG TTAAATTCCT TGCTCCAACC ACAAAAGCTT 
ACCACAGTAG AGATGATGGC ATCTCACATG TATACTTCAT ACAATAAACA TATTAGTCTG 
CACAAGGCTT TCACTGAAGT ATTTCTGGAT GATTCACATA ATCCTGCAAA TTGTCGGATA 
TCCGTACAAG TTGCCACACC AGCTTTAAAC CTTTCTGTTC GCTTCCCAAT ACCTGATCTT 
CGGTCAGATC AAGAAAGAGG ACCATGGTTT AAGAAGTCAC TTCAGAAGGA GATGCTTCAT 
TTAGCATTCA CAGACCTAGA ATTTAAGACT GAATTTATAG GAGGATCAAC CCCAGAGCAA 
ATTAAATTGG AACTTACCTT TAGAGAACTA GTTGGATCAT TCCAGGAAGA TAGAGGAGAA 
CCATCTATTA AGTTTTTCCA TGTGTCTAGT GGAGTAGATG GAGATACAGC ATCATCCGAT 
GACTTTGACT GGCCACGAAT TGTACTCAAA TTAAATCCGC CAGCTGTGCA TTCCATTTTG 
GAGCGAATAG CTGCGGAGGA AGAAGAGAGT GATGGCCGCT ACCAGGAAGA GGAGGAAGGG 
GGTGCTCATT CCCTGAAGGA TGTTTGTGAT CTGCGAAGAC CGGCCCCATC TCCCTTCTCC 
TCTCGGAGAG TCATGTTTGA AAATGAGCAG ATGGTGATGC CTGGAGACCC TGTAGAAATG 
ACAGAGTTTC AGGATAAAGC AATCAGCAAT TCTCACTATG TGCTGGAACT TACGTTACCC 
AACATTCATA TAACACTACC TAATAAAAGC TTTTATGAGA AGCTTTATAA TAGGATCTTT 
AATGACTTGC TGCTGTGGGA ACCAACAGCT CCTTCACCAG TGGAGACATT TGAAAATATT 
TCTTATGGTC TTGGTCTTTC TGTAGCCAGT CAGCTGATTA ACACCTTCAA CAAAGAGAGT 
TTTAGTCCAT TTAAATCCAC AGTTCACTAT GATGAGGAAA GTGGATCTGA GGAGGAGACG 
TTGCAGTATT TTTCTACAGT GGATCCCAAC TATCGTTCCC GTAGGAAAAA AAAATTAGAC 
TCTCAGAACA AGAACTCACA GAGTTTTCTC TCTGTTCTTC TGAGTATCAA TCATGGATTA 
ATGGCAGTGT TTACAGATGT CAGGCAAGAT AATGGAGAGC TGTTGGAAAA TAAGCATGGC 
GAGTTCTGGT TAGAGTTCAA CAGTGGTTCC TTGTTTTGTG TGACCAAGTA TGAAGGCTGT 
GATGACAAGC ACTACATCTG CCTTCATGCC AGCAGTCTCA GTCTATACCA CAAAGGCGTA 
GTAGATGGAG TGATTCTTCC TACAGAAACA CGACTGCCTA GTTCAACCCG CCCACACTGG 
TTGGAACCTA CTATTTATTC CTCTGAAGAA GATGGCCTCA GTAGAGCTTC TTCAGATGGT 
GTTGGAGGAG ACACTTTGAA TATGCTCTCT GTTGCAGTTA AAATACTTTC TGATAAATCA 
GAGTCCAATA CAAAGGAATT TCTCATTGCT GTGGGGTTGA AAGGAGCCAC ACTCCAGCAC 
AGAATACTTC CTTCTGGGCT TAGCTGGCAC GAGCAGATTT TATACTTCTT GAATATTGCT 
GATGAACCTG TTCTGGGATA TAATCCTCCA ACTTCGTTTA CAACTTTTCA TGTTCATCTC 
TGGAGCTGCG CACTTGATTA CAGGCCTCTT TATTTGCCGA TCCGATCTCT TCTCACTGTG 
GAAACCTTCA GCGTTTCAAG TAGCGTGGCC TTGGACAAAT CCTCGTCTAC TCTCAGGATA 
ATCTTGGATG AAGCTGCTTT ACATCTGTCT GACAAGTGTA ATACTGTCAC TATTAATTTG 
AGTAGAGATT ACGTTCGTGT GATGGATATG GGGCTTTTGG AATTAACCAT AACTGCAATG 
AAATCTGATT CTGATGGAGA GCAAACTGAG CCACGCTTCG AGCTACACTG TTCTAGTGAC 
ATTGTCCATA TCCGAACGTG CTCAGACTCT TGTGCTGCGT TAATGAACCT CATTCAATAC 
ATTGCAAGCT ATGGGGACTT ACATGCAGCG AGCAAGGTAG AGATGAAGCC TGGAGCCCCT 
CACCGAAAGA TGAAGGTAGA GCCAGCAGCG TCGTTCATTA GAACTAACTC ACCCCTGACA 
CCTTGCAGCC ACGTAGATTC CAGTGGTCGT TCATCCTCAC GAGGTCCAGT CCTTCCTGAA 
GCCGACCAGC AGATGTTACG TGATTTGATG AGTGACGCTA TGGAAGAGGT TGATCTGCAA 
CAGACTGCTA CGTCTACGCA AGCCCAGGCT AACGGTGTTT TGGATGAAAA ATCTCAAATT 
CAGGAGCCTT GTTGTTCAGA CCTCTTCCTG TTTCCAGATG AGAGTGGGAA TGTGTCCCAG 
GAGCCTGGCC CTCCTTATAC CTCATTCACA CCCCACTCGA TTAGTGATGC AATGGCAGGT 
GTGCCTACTG AGAATGATGA CTTTTGCATT CTTTTTGCAC CAAGAGCAAA CAGTCAGGAT 
AAGGAAGAAG AGCCCATTGT AAAAGTAATG GTTGATGATG CAATTGTAAT AAGAGAGAAT 
TACTTTAGTC AGCCTGTTAA GAAGACCGAT ACAAGCAAAG CCCCATTGCA TTTTCCGGTC 
CCTGTCGTCC GCTATGTTGT TAAAGAAGTC TCTCTTGTTT GGCATCTTTA TGGAGGAAAG 
GATTTTGGAA CAGCCCCTCC TACTTCTCCA GCTAAAAGTT ACATCAGTCC TCACAGCTCA 
CCTTCTCACA CACCCACAAG GCATGGACGC AACATGGTAT GTGGGGGAAA AGGAAGAAAC 
CATGACTTTT TAATGGAAAT ACAATTAAGC AAGGTGAAGT TTCAGCACGA GGTCTACCCG 
CCTTGCAAGC CAGAGTGTGA TTCTGGCCTC ATAGAGCACC CAGTCTCCCG GCAGGTGTTC 
ATTGTGCAGG ATCTTGAAAT TCGAGATCGT CTGGCAACAT CACAAATGAA TAAGTTTTTG 
TACCTGTATT GTAGTAAAGA AATGCCTCGA AAAGCTCATT CCAACATGCT GACAGTGAAA 
GCATTACATG TGCGACCGGA GTCTGGCAGG TCACCACAGG AATGCTGCTT GAGAGTATCC 
CTAATGCCAC TTCGCCTCAA TATTGACCAG GATGCCTTGT TCTTCCTGAA GGATTTCTTC 
ACAAGTCTTT CTACGGAAGT AGAGCTTCTA ATGGCTCCAG ATCCAGAAGT TAAAAAGTCT 
CCTGGAGCTG ATGTTACCTG CAGTTTGCCA AGGCACTTAA GCGCCTCCAA AGAGCCAAAT 
CTAGTTCTGT CTTTCCCTGG GCCAAAGCAG CCTTCCCAAG ATGATAGTGC CAATTCAGCG 
GAAGTGGTTA ATGGGGAAGA AGAGAAGGAC TTCTCAGCTG AAGAAACATC ATTTAGTGAT 
CAGCCCGTGT TTTTTAGAGA ATTTAGATTC ACATCAGAAG TACCTATTCG ACTTGATTAT 
CATGGCAAAC ACGTATCAAT GGATCAGGGT ACACTAGCTG GGATTCTGAT TGGTCTGGCC 
CAGTTAAACT GTTCAGAACT GAAGCTAAAG AGGCTTTTCT ACCGGCATGG CTTGCTAGGT 
GTTGACAAAT TGTTTTCATA TGCAATCACC GAATGGCTTA CTGACATTAA GAAGAATCAG 
CTACCAGGAA TCCTGGGAGG TGTGGGGCCT ATGCATTCAC TAGTACAATT AGTGCAAGGC 
CTAAAGGACT TGGTCTGGTT ACCGATAGAG CAATACCGGA AGGACGGTCG TATTGTCAGA 
GGGTTCCAGA GAGGCGCTGC CTCCTTTGGC ACCTCAACAG CGATGGCTGC TCTTGAGCTC 
ACAAACAGAA TGGTTCAGAC TATACAGGCA GCAGCAGAGA CTGCTTATGA TATGGTATCT 
CCTGGGACCC TTTCTATTGA GCCCAAGAAG ATCAAAAGGT TTCCTCATCA CCGCTTAGCC 
CACCAGCCAG TGGACCTGCG AGAAGGTGTG GCCAAGGCCT ACAGTGTTGT GAAAGAGGGA 
CTTACAGACA CAGCTCAGAC GATTTATGAA ACTGCAGCAC GAGAACATGA GAGCAGAGGC 
GTCACTGGTG CTGTGGGCGA GGTCCTGCGC CAGATTCCTC CCGCAGTGGT GAAACCTCTG 
ATTGTGGCCA CAGAAGCAAC ATCAAATGTA TTAGGTGGCA TGAGAAACCA GATTAGACCA 
GATGTTCGGC AAGACGAGTC ACAGAAATGG CGCCATGGGG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_014116145.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971620.1 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X2, mRNA.

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CloneID	OCe38046
Clone ID Related Accession (Same CDS sequence)	XM_014116145.2 , XM_014116145.1
Accession Version	XM_014116145.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	autophagy-related protein 2 homolog B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGTTTTCTT CTCCATGGCT GATATGGACA TGTCTCATAG TCTCTCTTCT 
CTTCCACCTC TGGGAGACCC TCCAAATATG GACCTTGAGT TATCATTAAC TAGTACATAC 
ACAAATACTC CAGCAGGATC TCCATTAAGT GCTACTGTGC TTCAGCCAAC GTGGGGAGAT 
TTCCTTGATC ATCATAAAGA ACAACCAGTA AGAGGGTCCA CCTTGCCATC CAACCTAGTT 
CACCCACCAA ATTTACAGAA GACTTCTCTC CCATCTAGAT CTGTTTCAGT GGATGAATCC 
AGGCCTGAAC TTATTTTTAG ACTAGCTGTG GGAACCTTGT CCATCTCTGT GCTTCACATC 
GATCCTTTAT CACCACCTGA AACATCACTC AACCTCAATC CATTGACACC TTTGGCGATA 
GCTTTCTTTA CCTGCATAGA AAATATTGAC CCAGCAAGAT TTTCAGCAGA TGATTTTAAG 
TCTCTCCGAG CAGTATTTGC GGAAGCTTGC AAACACGATC ACCTTCGATT TATAGGTACT 
GGCATCAAAG TGTCCTATGA ACAAAGACAA AGATCAGCTT CCCGATATTT TAGTACTGAC 
ATGTCCATCG GGCAAATGGA ACTTTTGGAG TGCCTATTTC CCACGGATTT TCATTCTGTT 
CCTCCTCACT ACACAGAGCT TTTAATGTTC CATTCCAAAG AACGAACTGA TTCCCATTCA 
CCTGTATGTC TTCAGCTTCA TTATAAGCAT TCTGAGAATA AAGGACCCCA GGGCAATCAA 
GCAAGACTTA GTTCTGTTCC TCAGAAGGCA GAATTACAAA TTAAGTTGAA TCCTGTGTTT 
TGTGAGCTGG ATATCAGTAT TGTGGACAGG TTAAATTCCT TGCTCCAACC ACAAAAGCTT 
ACCACAGTAG AGATGATGGC ATCTCACATG TATACTTCAT ACAATAAACA TATTAGTCTG 
CACAAGGCTT TCACTGAAGT ATTTCTGGAT GATTCACATA ATCCTGCAAA TTGTCGGATA 
TCCGTACAAG TTGCCACACC AGCTTTAAAC CTTTCTGTTC GCTTCCCAAT ACCTGATCTT 
CGGTCAGATC AAGAAAGAGG ACCATGGTTT AAGAAGTCAC TTCAGAAGGA GATGCTTCAT 
TTAGCATTCA CAGACCTAGA ATTTAAGACT GAATTTATAG GAGGATCAAC CCCAGAGCAA 
ATTAAATTGG AACTTACCTT TAGAGAACTA GTTGGATCAT TCCAGGAAGA TAGAGGAGAA 
CCATCTATTA AGTTTTTCCA TGTGTCTAGT GGAGTAGATG GAGATACAGC ATCATCCGAT 
GACTTTGACT GGCCACGAAT TGTACTCAAA TTAAATCCGC CAGCTGTGCA TTCCATTTTG 
GAGCGAATAG CTGCGGAGGA AGAAGAGAGT GATGGCCGCT ACCAGGAAGA GGAGGAAGGG 
GGTGCTCATT CCCTGAAGGA TGTTTGTGAT CTGCGAAGAC CGGCCCCATC TCCCTTCTCC 
TCTCGGAGAG TCATGTTTGA AAATGAGCAG ATGGTGATGC CTGGAGACCC TGTAGAAATG 
ACAGAGTTTC AGGATAAAGC AATCAGCAAT TCTCACTATG TGCTGGAACT TACGTTACCC 
AACATTCATA TAACACTACC TAATAAAAGC TTTTATGAGA AGCTTTATAA TAGGATCTTT 
AATGACTTGC TGCTGTGGGA ACCAACAGCT CCTTCACCAG TGGAGACATT TGAAAATATT 
TCTTATGGTC TTGGTCTTTC TGTAGCCAGT CAGCTGATTA ACACCTTCAA CAAAGAGAGT 
TTTAGTCCAT TTAAATCCAC AGTTCACTAT GATGAGGAAA GTGGATCTGA GGAGGAGACG 
TTGCAGTATT TTTCTACAGT GGATCCCAAC TATCGTTCCC GTAGGAAAAA AAAATTAGAC 
TCTCAGAACA AGAACTCACA GAGTTTTCTC TCTGTTCTTC TGAGTATCAA TCATGGATTA 
ATGGCAGTGT TTACAGATGT CAGGCAAGAT AATGGAGAGC TGTTGGAAAA TAAGCATGGC 
GAGTTCTGGT TAGAGTTCAA CAGTGGTTCC TTGTTTTGTG TGACCAAGTA TGAAGGCTGT 
GATGACAAGC ACTACATCTG CCTTCATGCC AGCAGTCTCA GTCTATACCA CAAAGGCGTA 
GTAGATGGAG TGATTCTTCC TACAGAAACA CGACTGCCTA GTTCAACCCG CCCACACTGG 
TTGGAACCTA CTATTTATTC CTCTGAAGAA GATGGCCTCA GTAGAGCTTC TTCAGATGGT 
GTTGGAGGAG ACACTTTGAA TATGCTCTCT GTTGCAGTTA AAATACTTTC TGATAAATCA 
GAGTCCAATA CAAAGGAATT TCTCATTGCT GTGGGGTTGA AAGGAGCCAC ACTCCAGCAC 
AGAATACTTC CTTCTGGGCT TAGCTGGCAC GAGCAGATTT TATACTTCTT GAATATTGCT 
GATGAACCTG TTCTGGGATA TAATCCTCCA ACTTCGTTTA CAACTTTTCA TGTTCATCTC 
TGGAGCTGCG CACTTGATTA CAGGCCTCTT TATTTGCCGA TCCGATCTCT TCTCACTGTG 
GAAACCTTCA GCGTTTCAAG TAGCGTGGCC TTGGACAAAT CCTCGTCTAC TCTCAGGATA 
ATCTTGGATG AAGCTGCTTT ACATCTGTCT GACAAGTGTA ATACTGTCAC TATTAATTTG 
AGTAGAGATT ACGTTCGTGT GATGGATATG GGGCTTTTGG AATTAACCAT AACTGCAATG 
AAATCTGATT CTGATGGAGA GCAAACTGAG CCACGCTTCG AGCTACACTG TTCTAGTGAC 
ATTGTCCATA TCCGAACGTG CTCAGACTCT TGTGCTGCGT TAATGAACCT CATTCAATAC 
ATTGCAAGCT ATGGGGACTT ACATGCAGCG AGCAAGGTAG AGATGAAGCC TGGAGCCCCT 
CACCGAAAGA TGAAGGTAGA GCCAGCAGCG TCGTTCATTA GAACTAACTC ACCCCTGACA 
CCTTGCAGCC ACGTAGATTC CAGTGGTCGT TCATCCTCAC GAGGTCCAGT CCTTCCTGAA 
GCCGACCAGC AGATGTTACG TGATTTGATG AGTGACGCTA TGGAAGAGGT TGATCTGCAA 
CAGACTGCTA CGTCTACGCA AGCCCAGGCT AACGGTGTTT TGGATGAAAA ATCTCAAATT 
CAGGAGCCTT GTTGTTCAGA CCTCTTCCTG TTTCCAGATG AGAGTGGGAA TGTGTCCCAG 
GAGCCTGGCC CTCCTTATAC CTCATTCACA CCCCACTCGA TTAGTGATGC AATGGCAGGT 
GTGCCTACTG AGAATGATGA CTTTTGCATT CTTTTTGCAC CAAGAGCAAA CAGTCAGGAT 
AAGGAAGAAG AGCCCATTGT AAAAGTAATG GTTGATGATG CAATTGTAAT AAGAGAGAAT 
TACTTTAGTC AGCCTGTTAA GAAGACCGAT ACAAGCAAAG CCCCATTGCA TTTTCCGGTC 
CCTGTCGTCC GCTATGTTGT TAAAGAAGTC TCTCTTGTTT GGCATCTTTA TGGAGGAAAG 
GATTTTGGAA CAGCCCCTCC TACTTCTCCA GCTAAAAGTT ACATCAGTCC TCACAGCTCA 
CCTTCTCACA CACCCACAAG GCATGGACGC AACATGGTAT GTGGGGGAAA AGGAAGAAAC 
CATGACTTTT TAATGGAAAT ACAATTAAGC AAGGTGAAGT TTCAGCACGA GGTCTACCCG 
CCTTGCAAGC CAGAGTGTGA TTCTGGCCTC ATAGAGCACC CAGTCTCCCG GCAGGTGTTC 
ATTGTGCAGG ATCTTGAAAT TCGAGATCGT CTGGCAACAT CACAAATGAA TAAGTTTTTG 
TACCTGTATT GTAGTAAAGA AATGCCTCGA AAAGCTCATT CCAACATGCT GACAGTGAAA 
GCATTACATG TGCGACCGGA GTCTGGCAGG TCACCACAGG AATGCTGCTT GAGAGTATCC 
CTAATGCCAC TTCGCCTCAA TATTGACCAG GATGCCTTGT TCTTCCTGAA GGATTTCTTC 
ACAAGTCTTT CTACGGAAGT AGAGCTTCTA ATGGCTCCAG ATCCAGAAGT TAAAAAGTCT 
CCTGGAGCTG ATGTTACCTG CAGTTTGCCA AGGCACTTAA GCGCCTCCAA AGAGCCAAAT 
CTAGTTCTGT CTTTCCCTGG GCCAAAGCAG CCTTCCCAAG ATGATAGTGC CAATTCAGCG 
GAAGTGGTTA ATGGGGAAGA AGAGAAGGAC TTCTCAGCTG AAGAAACATC ATTTAGTGAT 
CAGCCCGTGT TTTTTAGAGA ATTTAGATTC ACATCAGAAG TACCTATTCG ACTTGATTAT 
CATGGCAAAC ACGTATCAAT GGATCAGGGT ACACTAGCTG GGATTCTGAT TGGTCTGGCC 
CAGTTAAACT GTTCAGAACT GAAGCTAAAG AGGCTTTTCT ACCGGCATGG CTTGCTAGGT 
GTTGACAAAT TGTTTTCATA TGCAATCACC GAATGGCTTA CTGACATTAA GAAGAATCAG 
CTACCAGGAA TCCTGGGAGG TGTGGGGCCT ATGCATTCAC TAGTACAATT AGTGCAAGGC 
CTAAAGGACT TGGTCTGGTT ACCGATAGAG CAATACCGGA AGGACGGTCG TATTGTCAGA 
GGGTTCCAGA GAGGCGCTGC CTCCTTTGGC ACCTCAACAG CGATGGCTGC TCTTGAGCTC 
ACAAACAGAA TGGTTCAGAC TATACAGGCA GCAGCAGAGA CTGCTTATGA TATGGTATCT 
CCTGGGACCC TTTCTATTGA GCCCAAGAAG ATCAAAAGGT TTCCTCATCA CCGCTTAGCC 
CACCAGCCAG TGGACCTGCG AGAAGGTGTG GCCAAGGCCT ACAGTGTTGT GAAAGAGGGA 
CTTACAGACA CAGCTCAGAC GATTTATGAA ACTGCAGCAC GAGAACATGA GAGCAGAGGC 
GTCACTGGTG CTGTGGGCGA GGTCCTGCGC CAGATTCCTC CCGCAGTGGT GAAACCTCTG 
ATTGTGGCCA CAGAAGCAAC ATCAAATGTA TTAGGTGGCA TGAGAAACCA GATTAGACCA 
GATGTTCGGC AAGACGAGTC ACAGAAATGG CGCCATGGGG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971620.1
CDS	2..6289
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVEPAASFIRTNSPLTPCSHVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_014116145.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014116145.1

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGTTTTCTT CTCCATGGCT GATATGGACA TGTCTCATAG TCTCTCTTCT 
CTTCCACCTC TGGGAGACCC TCCAAATATG GACCTTGAGT TATCATTAAC TAGTACATAC 
ACAAATACTC CAGCAGGATC TCCATTAAGT GCTACTGTGC TTCAGCCAAC GTGGGGAGAT 
TTCCTTGATC ATCATAAAGA ACAACCAGTA AGAGGGTCCA CCTTGCCATC CAACCTAGTT 
CACCCACCAA ATTTACAGAA GACTTCTCTC CCATCTAGAT CTGTTTCAGT GGATGAATCC 
AGGCCTGAAC TTATTTTTAG ACTAGCTGTG GGAACCTTGT CCATCTCTGT GCTTCACATC 
GATCCTTTAT CACCACCTGA AACATCACTC AACCTCAATC CATTGACACC TTTGGCGATA 
GCTTTCTTTA CCTGCATAGA AAATATTGAC CCAGCAAGAT TTTCAGCAGA TGATTTTAAG 
TCTCTCCGAG CAGTATTTGC GGAAGCTTGC AAACACGATC ACCTTCGATT TATAGGTACT 
GGCATCAAAG TGTCCTATGA ACAAAGACAA AGATCAGCTT CCCGATATTT TAGTACTGAC 
ATGTCCATCG GGCAAATGGA ACTTTTGGAG TGCCTATTTC CCACGGATTT TCATTCTGTT 
CCTCCTCACT ACACAGAGCT TTTAATGTTC CATTCCAAAG AACGAACTGA TTCCCATTCA 
CCTGTATGTC TTCAGCTTCA TTATAAGCAT TCTGAGAATA AAGGACCCCA GGGCAATCAA 
GCAAGACTTA GTTCTGTTCC TCAGAAGGCA GAATTACAAA TTAAGTTGAA TCCTGTGTTT 
TGTGAGCTGG ATATCAGTAT TGTGGACAGG TTAAATTCCT TGCTCCAACC ACAAAAGCTT 
ACCACAGTAG AGATGATGGC ATCTCACATG TATACTTCAT ACAATAAACA TATTAGTCTG 
CACAAGGCTT TCACTGAAGT ATTTCTGGAT GATTCACATA ATCCTGCAAA TTGTCGGATA 
TCCGTACAAG TTGCCACACC AGCTTTAAAC CTTTCTGTTC GCTTCCCAAT ACCTGATCTT 
CGGTCAGATC AAGAAAGAGG ACCATGGTTT AAGAAGTCAC TTCAGAAGGA GATGCTTCAT 
TTAGCATTCA CAGACCTAGA ATTTAAGACT GAATTTATAG GAGGATCAAC CCCAGAGCAA 
ATTAAATTGG AACTTACCTT TAGAGAACTA GTTGGATCAT TCCAGGAAGA TAGAGGAGAA 
CCATCTATTA AGTTTTTCCA TGTGTCTAGT GGAGTAGATG GAGATACAGC ATCATCCGAT 
GACTTTGACT GGCCACGAAT TGTACTCAAA TTAAATCCGC CAGCTGTGCA TTCCATTTTG 
GAGCGAATAG CTGCGGAGGA AGAAGAGAGT GATGGCCGCT ACCAGGAAGA GGAGGAAGGG 
GGTGCTCATT CCCTGAAGGA TGTTTGTGAT CTGCGAAGAC CGGCCCCATC TCCCTTCTCC 
TCTCGGAGAG TCATGTTTGA AAATGAGCAG ATGGTGATGC CTGGAGACCC TGTAGAAATG 
ACAGAGTTTC AGGATAAAGC AATCAGCAAT TCTCACTATG TGCTGGAACT TACGTTACCC 
AACATTCATA TAACACTACC TAATAAAAGC TTTTATGAGA AGCTTTATAA TAGGATCTTT 
AATGACTTGC TGCTGTGGGA ACCAACAGCT CCTTCACCAG TGGAGACATT TGAAAATATT 
TCTTATGGTC TTGGTCTTTC TGTAGCCAGT CAGCTGATTA ACACCTTCAA CAAAGAGAGT 
TTTAGTCCAT TTAAATCCAC AGTTCACTAT GATGAGGAAA GTGGATCTGA GGAGGAGACG 
TTGCAGTATT TTTCTACAGT GGATCCCAAC TATCGTTCCC GTAGGAAAAA AAAATTAGAC 
TCTCAGAACA AGAACTCACA GAGTTTTCTC TCTGTTCTTC TGAGTATCAA TCATGGATTA 
ATGGCAGTGT TTACAGATGT CAGGCAAGAT AATGGAGAGC TGTTGGAAAA TAAGCATGGC 
GAGTTCTGGT TAGAGTTCAA CAGTGGTTCC TTGTTTTGTG TGACCAAGTA TGAAGGCTGT 
GATGACAAGC ACTACATCTG CCTTCATGCC AGCAGTCTCA GTCTATACCA CAAAGGCGTA 
GTAGATGGAG TGATTCTTCC TACAGAAACA CGACTGCCTA GTTCAACCCG CCCACACTGG 
TTGGAACCTA CTATTTATTC CTCTGAAGAA GATGGCCTCA GTAGAGCTTC TTCAGATGGT 
GTTGGAGGAG ACACTTTGAA TATGCTCTCT GTTGCAGTTA AAATACTTTC TGATAAATCA 
GAGTCCAATA CAAAGGAATT TCTCATTGCT GTGGGGTTGA AAGGAGCCAC ACTCCAGCAC 
AGAATACTTC CTTCTGGGCT TAGCTGGCAC GAGCAGATTT TATACTTCTT GAATATTGCT 
GATGAACCTG TTCTGGGATA TAATCCTCCA ACTTCGTTTA CAACTTTTCA TGTTCATCTC 
TGGAGCTGCG CACTTGATTA CAGGCCTCTT TATTTGCCGA TCCGATCTCT TCTCACTGTG 
GAAACCTTCA GCGTTTCAAG TAGCGTGGCC TTGGACAAAT CCTCGTCTAC TCTCAGGATA 
ATCTTGGATG AAGCTGCTTT ACATCTGTCT GACAAGTGTA ATACTGTCAC TATTAATTTG 
AGTAGAGATT ACGTTCGTGT GATGGATATG GGGCTTTTGG AATTAACCAT AACTGCAATG 
AAATCTGATT CTGATGGAGA GCAAACTGAG CCACGCTTCG AGCTACACTG TTCTAGTGAC 
ATTGTCCATA TCCGAACGTG CTCAGACTCT TGTGCTGCGT TAATGAACCT CATTCAATAC 
ATTGCAAGCT ATGGGGACTT ACATGCAGCG AGCAAGGTAG AGATGAAGCC TGGAGCCCCT 
CACCGAAAGA TGAAGGTAGA GCCAGCAGCG TCGTTCATTA GAACTAACTC ACCCCTGACA 
CCTTGCAGCC ACGTAGATTC CAGTGGTCGT TCATCCTCAC GAGGTCCAGT CCTTCCTGAA 
GCCGACCAGC AGATGTTACG TGATTTGATG AGTGACGCTA TGGAAGAGGT TGATCTGCAA 
CAGACTGCTA CGTCTACGCA AGCCCAGGCT AACGGTGTTT TGGATGAAAA ATCTCAAATT 
CAGGAGCCTT GTTGTTCAGA CCTCTTCCTG TTTCCAGATG AGAGTGGGAA TGTGTCCCAG 
GAGCCTGGCC CTCCTTATAC CTCATTCACA CCCCACTCGA TTAGTGATGC AATGGCAGGT 
GTGCCTACTG AGAATGATGA CTTTTGCATT CTTTTTGCAC CAAGAGCAAA CAGTCAGGAT 
AAGGAAGAAG AGCCCATTGT AAAAGTAATG GTTGATGATG CAATTGTAAT AAGAGAGAAT 
TACTTTAGTC AGCCTGTTAA GAAGACCGAT ACAAGCAAAG CCCCATTGCA TTTTCCGGTC 
CCTGTCGTCC GCTATGTTGT TAAAGAAGTC TCTCTTGTTT GGCATCTTTA TGGAGGAAAG 
GATTTTGGAA CAGCCCCTCC TACTTCTCCA GCTAAAAGTT ACATCAGTCC TCACAGCTCA 
CCTTCTCACA CACCCACAAG GCATGGACGC AACATGGTAT GTGGGGGAAA AGGAAGAAAC 
CATGACTTTT TAATGGAAAT ACAATTAAGC AAGGTGAAGT TTCAGCACGA GGTCTACCCG 
CCTTGCAAGC CAGAGTGTGA TTCTGGCCTC ATAGAGCACC CAGTCTCCCG GCAGGTGTTC 
ATTGTGCAGG ATCTTGAAAT TCGAGATCGT CTGGCAACAT CACAAATGAA TAAGTTTTTG 
TACCTGTATT GTAGTAAAGA AATGCCTCGA AAAGCTCATT CCAACATGCT GACAGTGAAA 
GCATTACATG TGCGACCGGA GTCTGGCAGG TCACCACAGG AATGCTGCTT GAGAGTATCC 
CTAATGCCAC TTCGCCTCAA TATTGACCAG GATGCCTTGT TCTTCCTGAA GGATTTCTTC 
ACAAGTCTTT CTACGGAAGT AGAGCTTCTA ATGGCTCCAG ATCCAGAAGT TAAAAAGTCT 
CCTGGAGCTG ATGTTACCTG CAGTTTGCCA AGGCACTTAA GCGCCTCCAA AGAGCCAAAT 
CTAGTTCTGT CTTTCCCTGG GCCAAAGCAG CCTTCCCAAG ATGATAGTGC CAATTCAGCG 
GAAGTGGTTA ATGGGGAAGA AGAGAAGGAC TTCTCAGCTG AAGAAACATC ATTTAGTGAT 
CAGCCCGTGT TTTTTAGAGA ATTTAGATTC ACATCAGAAG TACCTATTCG ACTTGATTAT 
CATGGCAAAC ACGTATCAAT GGATCAGGGT ACACTAGCTG GGATTCTGAT TGGTCTGGCC 
CAGTTAAACT GTTCAGAACT GAAGCTAAAG AGGCTTTTCT ACCGGCATGG CTTGCTAGGT 
GTTGACAAAT TGTTTTCATA TGCAATCACC GAATGGCTTA CTGACATTAA GAAGAATCAG 
CTACCAGGAA TCCTGGGAGG TGTGGGGCCT ATGCATTCAC TAGTACAATT AGTGCAAGGC 
CTAAAGGACT TGGTCTGGTT ACCGATAGAG CAATACCGGA AGGACGGTCG TATTGTCAGA 
GGGTTCCAGA GAGGCGCTGC CTCCTTTGGC ACCTCAACAG CGATGGCTGC TCTTGAGCTC 
ACAAACAGAA TGGTTCAGAC TATACAGGCA GCAGCAGAGA CTGCTTATGA TATGGTATCT 
CCTGGGACCC TTTCTATTGA GCCCAAGAAG ATCAAAAGGT TTCCTCATCA CCGCTTAGCC 
CACCAGCCAG TGGACCTGCG AGAAGGTGTG GCCAAGGCCT ACAGTGTTGT GAAAGAGGGA 
CTTACAGACA CAGCTCAGAC GATTTATGAA ACTGCAGCAC GAGAACATGA GAGCAGAGGC 
GTCACTGGTG CTGTGGGCGA GGTCCTGCGC CAGATTCCTC CCGCAGTGGT GAAACCTCTG 
ATTGTGGCCA CAGAAGCAAC ATCAAATGTA TTAGGTGGCA TGAGAAACCA GATTAGACCA 
GATGTTCGGC AAGACGAGTC ACAGAAATGG CGCCATGGGG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_537549.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537549.3 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X4, mRNA.

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CloneID	OCe10801
Clone ID Related Accession (Same CDS sequence)	XM_537549.5 , XM_537549.4
Accession Version	XM_537549.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	autophagy-related protein 2 homolog B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537549.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGATTCCAGT GGTCGTTCAT CCTCACGAGG TCCAGTCCTT 
CCTGAAGCCG ACCAGCAGAT GTTACGTGAT TTGATGAGTG ACGCTATGGA AGAGGTTGAT 
CTGCAACAGA CTGCTACGTC TACGCAAGCC CAGGCTAACG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCTTGTTG TTCAGACCTC TTCCTGTTTC CAGATGAGAG TGGGAATGTG 
TCCCAGGAGC CTGGCCCTCC TTATACCTCA TTCACACCCC ACTCGATTAG TGATGCAATG 
GCAGGTGTGC CTACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAG AGCAAACAGT 
CAGGATAAGG AAGAAGAGCC CATTGTAAAA GTAATGGTTG ATGATGCAAT TGTAATAAGA 
GAGAATTACT TTAGTCAGCC TGTTAAGAAG ACCGATACAA GCAAAGCCCC ATTGCATTTT 
CCGGTCCCTG TCGTCCGCTA TGTTGTTAAA GAAGTCTCTC TTGTTTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAACAGC CCCTCCTACT TCTCCAGCTA AAAGTTACAT CAGTCCTCAC 
AGCTCACCTT CTCACACACC CACAAGGCAT GGACGCAACA TGGTATGTGG GGGAAAAGGA 
AGAAACCATG ACTTTTTAAT GGAAATACAA TTAAGCAAGG TGAAGTTTCA GCACGAGGTC 
TACCCGCCTT GCAAGCCAGA GTGTGATTCT GGCCTCATAG AGCACCCAGT CTCCCGGCAG 
GTGTTCATTG TGCAGGATCT TGAAATTCGA GATCGTCTGG CAACATCACA AATGAATAAG 
TTTTTGTACC TGTATTGTAG TAAAGAAATG CCTCGAAAAG CTCATTCCAA CATGCTGACA 
GTGAAAGCAT TACATGTGCG ACCGGAGTCT GGCAGGTCAC CACAGGAATG CTGCTTGAGA 
GTATCCCTAA TGCCACTTCG CCTCAATATT GACCAGGATG CCTTGTTCTT CCTGAAGGAT 
TTCTTCACAA GTCTTTCTAC GGAAGTAGAG CTTCTAATGG CTCCAGATCC AGAAGTTAAA 
AAGTCTCCTG GAGCTGATGT TACCTGCAGT TTGCCAAGGC ACTTAAGCGC CTCCAAAGAG 
CCAAATCTAG TTCTGTCTTT CCCTGGGCCA AAGCAGCCTT CCCAAGATGA TAGTGCCAAT 
TCAGCGGAAG TGGTTAATGG GGAAGAAGAG AAGGACTTCT CAGCTGAAGA AACATCATTT 
AGTGATCAGC CCGTGTTTTT TAGAGAATTT AGATTCACAT CAGAAGTACC TATTCGACTT 
GATTATCATG GCAAACACGT ATCAATGGAT CAGGGTACAC TAGCTGGGAT TCTGATTGGT 
CTGGCCCAGT TAAACTGTTC AGAACTGAAG CTAAAGAGGC TTTTCTACCG GCATGGCTTG 
CTAGGTGTTG ACAAATTGTT TTCATATGCA ATCACCGAAT GGCTTACTGA CATTAAGAAG 
AATCAGCTAC CAGGAATCCT GGGAGGTGTG GGGCCTATGC ATTCACTAGT ACAATTAGTG 
CAAGGCCTAA AGGACTTGGT CTGGTTACCG ATAGAGCAAT ACCGGAAGGA CGGTCGTATT 
GTCAGAGGGT TCCAGAGAGG CGCTGCCTCC TTTGGCACCT CAACAGCGAT GGCTGCTCTT 
GAGCTCACAA ACAGAATGGT TCAGACTATA CAGGCAGCAG CAGAGACTGC TTATGATATG 
GTATCTCCTG GGACCCTTTC TATTGAGCCC AAGAAGATCA AAAGGTTTCC TCATCACCGC 
TTAGCCCACC AGCCAGTGGA CCTGCGAGAA GGTGTGGCCA AGGCCTACAG TGTTGTGAAA 
GAGGGACTTA CAGACACAGC TCAGACGATT TATGAAACTG CAGCACGAGA ACATGAGAGC 
AGAGGCGTCA CTGGTGCTGT GGGCGAGGTC CTGCGCCAGA TTCCTCCCGC AGTGGTGAAA 
CCTCTGATTG TGGCCACAGA AGCAACATCA AATGTATTAG GTGGCATGAG AAACCAGATT 
AGACCAGATG TTCGGCAAGA CGAGTCACAG AAATGGCGCC ATGGGGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537549.3
CDS	16..6249
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_537549.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537549.5

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGATTCCAGT GGTCGTTCAT CCTCACGAGG TCCAGTCCTT 
CCTGAAGCCG ACCAGCAGAT GTTACGTGAT TTGATGAGTG ACGCTATGGA AGAGGTTGAT 
CTGCAACAGA CTGCTACGTC TACGCAAGCC CAGGCTAACG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCTTGTTG TTCAGACCTC TTCCTGTTTC CAGATGAGAG TGGGAATGTG 
TCCCAGGAGC CTGGCCCTCC TTATACCTCA TTCACACCCC ACTCGATTAG TGATGCAATG 
GCAGGTGTGC CTACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAG AGCAAACAGT 
CAGGATAAGG AAGAAGAGCC CATTGTAAAA GTAATGGTTG ATGATGCAAT TGTAATAAGA 
GAGAATTACT TTAGTCAGCC TGTTAAGAAG ACCGATACAA GCAAAGCCCC ATTGCATTTT 
CCGGTCCCTG TCGTCCGCTA TGTTGTTAAA GAAGTCTCTC TTGTTTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAACAGC CCCTCCTACT TCTCCAGCTA AAAGTTACAT CAGTCCTCAC 
AGCTCACCTT CTCACACACC CACAAGGCAT GGACGCAACA TGGTATGTGG GGGAAAAGGA 
AGAAACCATG ACTTTTTAAT GGAAATACAA TTAAGCAAGG TGAAGTTTCA GCACGAGGTC 
TACCCGCCTT GCAAGCCAGA GTGTGATTCT GGCCTCATAG AGCACCCAGT CTCCCGGCAG 
GTGTTCATTG TGCAGGATCT TGAAATTCGA GATCGTCTGG CAACATCACA AATGAATAAG 
TTTTTGTACC TGTATTGTAG TAAAGAAATG CCTCGAAAAG CTCATTCCAA CATGCTGACA 
GTGAAAGCAT TACATGTGCG ACCGGAGTCT GGCAGGTCAC CACAGGAATG CTGCTTGAGA 
GTATCCCTAA TGCCACTTCG CCTCAATATT GACCAGGATG CCTTGTTCTT CCTGAAGGAT 
TTCTTCACAA GTCTTTCTAC GGAAGTAGAG CTTCTAATGG CTCCAGATCC AGAAGTTAAA 
AAGTCTCCTG GAGCTGATGT TACCTGCAGT TTGCCAAGGC ACTTAAGCGC CTCCAAAGAG 
CCAAATCTAG TTCTGTCTTT CCCTGGGCCA AAGCAGCCTT CCCAAGATGA TAGTGCCAAT 
TCAGCGGAAG TGGTTAATGG GGAAGAAGAG AAGGACTTCT CAGCTGAAGA AACATCATTT 
AGTGATCAGC CCGTGTTTTT TAGAGAATTT AGATTCACAT CAGAAGTACC TATTCGACTT 
GATTATCATG GCAAACACGT ATCAATGGAT CAGGGTACAC TAGCTGGGAT TCTGATTGGT 
CTGGCCCAGT TAAACTGTTC AGAACTGAAG CTAAAGAGGC TTTTCTACCG GCATGGCTTG 
CTAGGTGTTG ACAAATTGTT TTCATATGCA ATCACCGAAT GGCTTACTGA CATTAAGAAG 
AATCAGCTAC CAGGAATCCT GGGAGGTGTG GGGCCTATGC ATTCACTAGT ACAATTAGTG 
CAAGGCCTAA AGGACTTGGT CTGGTTACCG ATAGAGCAAT ACCGGAAGGA CGGTCGTATT 
GTCAGAGGGT TCCAGAGAGG CGCTGCCTCC TTTGGCACCT CAACAGCGAT GGCTGCTCTT 
GAGCTCACAA ACAGAATGGT TCAGACTATA CAGGCAGCAG CAGAGACTGC TTATGATATG 
GTATCTCCTG GGACCCTTTC TATTGAGCCC AAGAAGATCA AAAGGTTTCC TCATCACCGC 
TTAGCCCACC AGCCAGTGGA CCTGCGAGAA GGTGTGGCCA AGGCCTACAG TGTTGTGAAA 
GAGGGACTTA CAGACACAGC TCAGACGATT TATGAAACTG CAGCACGAGA ACATGAGAGC 
AGAGGCGTCA CTGGTGCTGT GGGCGAGGTC CTGCGCCAGA TTCCTCCCGC AGTGGTGAAA 
CCTCTGATTG TGGCCACAGA AGCAACATCA AATGTATTAG GTGGCATGAG AAACCAGATT 
AGACCAGATG TTCGGCAAGA CGAGTCACAG AAATGGCGCC ATGGGGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATG2B ( XM_537549.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537549.3 Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X3, mRNA.

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CloneID	OCe10801
Clone ID Related Accession (Same CDS sequence)	XM_537549.5 , XM_537549.4
Accession Version	XM_537549.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	autophagy-related protein 2 homolog B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_537549.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGATTCCAGT GGTCGTTCAT CCTCACGAGG TCCAGTCCTT 
CCTGAAGCCG ACCAGCAGAT GTTACGTGAT TTGATGAGTG ACGCTATGGA AGAGGTTGAT 
CTGCAACAGA CTGCTACGTC TACGCAAGCC CAGGCTAACG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCTTGTTG TTCAGACCTC TTCCTGTTTC CAGATGAGAG TGGGAATGTG 
TCCCAGGAGC CTGGCCCTCC TTATACCTCA TTCACACCCC ACTCGATTAG TGATGCAATG 
GCAGGTGTGC CTACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAG AGCAAACAGT 
CAGGATAAGG AAGAAGAGCC CATTGTAAAA GTAATGGTTG ATGATGCAAT TGTAATAAGA 
GAGAATTACT TTAGTCAGCC TGTTAAGAAG ACCGATACAA GCAAAGCCCC ATTGCATTTT 
CCGGTCCCTG TCGTCCGCTA TGTTGTTAAA GAAGTCTCTC TTGTTTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAACAGC CCCTCCTACT TCTCCAGCTA AAAGTTACAT CAGTCCTCAC 
AGCTCACCTT CTCACACACC CACAAGGCAT GGACGCAACA TGGTATGTGG GGGAAAAGGA 
AGAAACCATG ACTTTTTAAT GGAAATACAA TTAAGCAAGG TGAAGTTTCA GCACGAGGTC 
TACCCGCCTT GCAAGCCAGA GTGTGATTCT GGCCTCATAG AGCACCCAGT CTCCCGGCAG 
GTGTTCATTG TGCAGGATCT TGAAATTCGA GATCGTCTGG CAACATCACA AATGAATAAG 
TTTTTGTACC TGTATTGTAG TAAAGAAATG CCTCGAAAAG CTCATTCCAA CATGCTGACA 
GTGAAAGCAT TACATGTGCG ACCGGAGTCT GGCAGGTCAC CACAGGAATG CTGCTTGAGA 
GTATCCCTAA TGCCACTTCG CCTCAATATT GACCAGGATG CCTTGTTCTT CCTGAAGGAT 
TTCTTCACAA GTCTTTCTAC GGAAGTAGAG CTTCTAATGG CTCCAGATCC AGAAGTTAAA 
AAGTCTCCTG GAGCTGATGT TACCTGCAGT TTGCCAAGGC ACTTAAGCGC CTCCAAAGAG 
CCAAATCTAG TTCTGTCTTT CCCTGGGCCA AAGCAGCCTT CCCAAGATGA TAGTGCCAAT 
TCAGCGGAAG TGGTTAATGG GGAAGAAGAG AAGGACTTCT CAGCTGAAGA AACATCATTT 
AGTGATCAGC CCGTGTTTTT TAGAGAATTT AGATTCACAT CAGAAGTACC TATTCGACTT 
GATTATCATG GCAAACACGT ATCAATGGAT CAGGGTACAC TAGCTGGGAT TCTGATTGGT 
CTGGCCCAGT TAAACTGTTC AGAACTGAAG CTAAAGAGGC TTTTCTACCG GCATGGCTTG 
CTAGGTGTTG ACAAATTGTT TTCATATGCA ATCACCGAAT GGCTTACTGA CATTAAGAAG 
AATCAGCTAC CAGGAATCCT GGGAGGTGTG GGGCCTATGC ATTCACTAGT ACAATTAGTG 
CAAGGCCTAA AGGACTTGGT CTGGTTACCG ATAGAGCAAT ACCGGAAGGA CGGTCGTATT 
GTCAGAGGGT TCCAGAGAGG CGCTGCCTCC TTTGGCACCT CAACAGCGAT GGCTGCTCTT 
GAGCTCACAA ACAGAATGGT TCAGACTATA CAGGCAGCAG CAGAGACTGC TTATGATATG 
GTATCTCCTG GGACCCTTTC TATTGAGCCC AAGAAGATCA AAAGGTTTCC TCATCACCGC 
TTAGCCCACC AGCCAGTGGA CCTGCGAGAA GGTGTGGCCA AGGCCTACAG TGTTGTGAAA 
GAGGGACTTA CAGACACAGC TCAGACGATT TATGAAACTG CAGCACGAGA ACATGAGAGC 
AGAGGCGTCA CTGGTGCTGT GGGCGAGGTC CTGCGCCAGA TTCCTCCCGC AGTGGTGAAA 
CCTCTGATTG TGGCCACAGA AGCAACATCA AATGTATTAG GTGGCATGAG AAACCAGATT 
AGACCAGATG TTCGGCAAGA CGAGTCACAG AAATGGCGCC ATGGGGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537549.3
CDS	2..6235
Translation	MPWPFSESIKKRACRYLLQRYLGHFLQEKLSLEQLSLDLYQGTGSLAQVPLDKWCLNEILESADAPLEVTEGFIQSISLSVPWGSLLQDNCALEVKGLEMVFRPRPRLATGSEPMYWSSFMTSSMQLAKECLSQKLTDEQGEGSQPFEGLEKFAETIETVLRRVTVTFIDTVLRIEHVPDNSKTGTALEIRIERTVYCDEAADESSGINVHQPTAFAHKLLQLSGVSLFWEEFSASAKSSPVCSTAPVETEPKLSPSWNPKIVYEPHPQLTRNLPEVAPSDPVQIGGLIGRLELSLTLKQNEVLPGAKLDIDGQIDSIHLFLSPRQVHLLLDMLAAIAGPENSSKIGLANKDRKNRPMQQEDEYRIQMELNRYYLRKDSLSVGVSSEQSFYETESARTPSSREEEVFFSMADMDMSHSLSSLPPLGDPPNMDLELSLTSTYTNTPAGSPLSATVLQPTWGDFLDHHKEQPVRGSTLPSNLVHPPNLQKTSLPSRSVSVDESRPELIFRLAVGTLSISVLHIDPLSPPETSLNLNPLTPLAIAFFTCIENIDPARFSADDFKSLRAVFAEACKHDHLRFIGTGIKVSYEQRQRSASRYFSTDMSIGQMELLECLFPTDFHSVPPHYTELLMFHSKERTDSHSPVCLQLHYKHSENKGPQGNQARLSSVPQKAELQIKLNPVFCELDISIVDRLNSLLQPQKLTTVEMMASHMYTSYNKHISLHKAFTEVFLDDSHNPANCRISVQVATPALNLSVRFPIPDLRSDQERGPWFKKSLQKEMLHLAFTDLEFKTEFIGGSTPEQIKLELTFRELVGSFQEDRGEPSIKFFHVSSGVDGDTASSDDFDWPRIVLKLNPPAVHSILERIAAEEEESDGRYQEEEEGGAHSLKDVCDLRRPAPSPFSSRRVMFENEQMVMPGDPVEMTEFQDKAISNSHYVLELTLPNIHITLPNKSFYEKLYNRIFNDLLLWEPTAPSPVETFENISYGLGLSVASQLINTFNKESFSPFKSTVHYDEESGSEEETLQYFSTVDPNYRSRRKKKLDSQNKNSQSFLSVLLSINHGLMAVFTDVRQDNGELLENKHGEFWLEFNSGSLFCVTKYEGCDDKHYICLHASSLSLYHKGVVDGVILPTETRLPSSTRPHWLEPTIYSSEEDGLSRASSDGVGGDTLNMLSVAVKILSDKSESNTKEFLIAVGLKGATLQHRILPSGLSWHEQILYFLNIADEPVLGYNPPTSFTTFHVHLWSCALDYRPLYLPIRSLLTVETFSVSSSVALDKSSSTLRIILDEAALHLSDKCNTVTINLSRDYVRVMDMGLLELTITAMKSDSDGEQTEPRFELHCSSDIVHIRTCSDSCAALMNLIQYIASYGDLHAASKVEMKPGAPHRKMKVDSSGRSSSRGPVLPEADQQMLRDLMSDAMEEVDLQQTATSTQAQANGVLDEKSQIQEPCCSDLFLFPDESGNVSQEPGPPYTSFTPHSISDAMAGVPTENDDFCILFAPRANSQDKEEEPIVKVMVDDAIVIRENYFSQPVKKTDTSKAPLHFPVPVVRYVVKEVSLVWHLYGGKDFGTAPPTSPAKSYISPHSSPSHTPTRHGRNMVCGGKGRNHDFLMEIQLSKVKFQHEVYPPCKPECDSGLIEHPVSRQVFIVQDLEIRDRLATSQMNKFLYLYCSKEMPRKAHSNMLTVKALHVRPESGRSPQECCLRVSLMPLRLNIDQDALFFLKDFFTSLSTEVELLMAPDPEVKKSPGADVTCSLPRHLSASKEPNLVLSFPGPKQPSQDDSANSAEVVNGEEEKDFSAEETSFSDQPVFFREFRFTSEVPIRLDYHGKHVSMDQGTLAGILIGLAQLNCSELKLKRLFYRHGLLGVDKLFSYAITEWLTDIKKNQLPGILGGVGPMHSLVQLVQGLKDLVWLPIEQYRKDGRIVRGFQRGAASFGTSTAMAALELTNRMVQTIQAAAETAYDMVSPGTLSIEPKKIKRFPHHRLAHQPVDLREGVAKAYSVVKEGLTDTAQTIYETAAREHESRGVTGAVGEVLRQIPPAVVKPLIVATEATSNVLGGMRNQIRPDVRQDESQKWRHGED

Target ORF information:

RefSeq Version	XM_537549.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris autophagy related 2B (ATG2B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537549.4

ORF Insert Sequence:

ATGCCTTGGC CGTTCTCGGA GTCCATCAAG AAGAGGGCAT GTCGCTACCT CCTGCAGAGA 
TACCTGGGCC ACTTCCTGCA GGAGAAGCTG AGCCTGGAGC AGCTCAGCCT GGACCTGTAC 
CAGGGCACCG GCTCCCTCGC CCAAGTCCCC TTGGACAAAT GGTGTCTCAA TGAGATCCTG 
GAGTCGGCAG ATGCACCTTT AGAAGTCACT GAAGGATTCA TACAGTCAAT TTCTCTGTCA 
GTTCCATGGG GCTCCTTGCT GCAGGATAAT TGTGCACTGG AAGTGAAAGG GCTAGAAATG 
GTCTTCCGGC CTAGACCCCG CCTAGCAACT GGTTCTGAGC CTATGTATTG GTCAAGTTTC 
ATGACCAGCA GTATGCAATT GGCAAAAGAA TGTCTTAGCC AGAAATTAAC AGATGAACAA 
GGAGAAGGAT CTCAGCCTTT TGAAGGACTT GAAAAGTTTG CTGAAACCAT TGAAACAGTA 
CTAAGAAGAG TAACAGTCAC TTTTATAGAC ACAGTCTTGA GAATTGAACA CGTGCCAGAC 
AATTCCAAAA CTGGAACTGC GCTTGAGATT CGAATAGAAA GAACTGTCTA CTGTGATGAA 
GCTGCTGACG AGTCCTCAGG AATTAATGTG CATCAGCCCA CAGCCTTTGC TCACAAGTTA 
CTTCAGCTCT CCGGAGTATC TCTCTTCTGG GAGGAGTTTT CTGCGTCAGC AAAATCTTCC 
CCAGTGTGTT CAACTGCACC AGTGGAAACT GAGCCAAAGC TGTCACCTAG CTGGAATCCC 
AAAATTGTTT ATGAGCCACA CCCACAGCTA ACTAGAAATT TACCAGAGGT AGCACCCTCT 
GACCCAGTGC AGATTGGAGG GCTAATTGGT AGGCTGGAGT TGAGTCTCAC ATTGAAACAG 
AATGAAGTAC TTCCTGGAGC TAAGTTGGAT ATTGATGGAC AGATAGACTC TATTCATCTA 
TTCCTGTCAC CAAGGCAGGT GCACTTGCTT TTGGATATGT TAGCAGCTAT TGCTGGACCA 
GAAAATTCTA GCAAAATAGG GTTAGCTAAT AAAGATAGAA AAAACCGACC CATGCAGCAG 
GAAGACGAAT ATCGAATTCA GATGGAATTA AACCGGTATT ATTTGAGAAA AGATTCCCTT 
TCGGTGGGTG TGTCTTCAGA GCAAAGTTTT TATGAGACAG AATCTGCTCG TACACCTTCT 
AGCCGTGAAG AAGAAGTTTT CTTCTCCATG GCTGATATGG ACATGTCTCA TAGTCTCTCT 
TCTCTTCCAC CTCTGGGAGA CCCTCCAAAT ATGGACCTTG AGTTATCATT AACTAGTACA 
TACACAAATA CTCCAGCAGG ATCTCCATTA AGTGCTACTG TGCTTCAGCC AACGTGGGGA 
GATTTCCTTG ATCATCATAA AGAACAACCA GTAAGAGGGT CCACCTTGCC ATCCAACCTA 
GTTCACCCAC CAAATTTACA GAAGACTTCT CTCCCATCTA GATCTGTTTC AGTGGATGAA 
TCCAGGCCTG AACTTATTTT TAGACTAGCT GTGGGAACCT TGTCCATCTC TGTGCTTCAC 
ATCGATCCTT TATCACCACC TGAAACATCA CTCAACCTCA ATCCATTGAC ACCTTTGGCG 
ATAGCTTTCT TTACCTGCAT AGAAAATATT GACCCAGCAA GATTTTCAGC AGATGATTTT 
AAGTCTCTCC GAGCAGTATT TGCGGAAGCT TGCAAACACG ATCACCTTCG ATTTATAGGT 
ACTGGCATCA AAGTGTCCTA TGAACAAAGA CAAAGATCAG CTTCCCGATA TTTTAGTACT 
GACATGTCCA TCGGGCAAAT GGAACTTTTG GAGTGCCTAT TTCCCACGGA TTTTCATTCT 
GTTCCTCCTC ACTACACAGA GCTTTTAATG TTCCATTCCA AAGAACGAAC TGATTCCCAT 
TCACCTGTAT GTCTTCAGCT TCATTATAAG CATTCTGAGA ATAAAGGACC CCAGGGCAAT 
CAAGCAAGAC TTAGTTCTGT TCCTCAGAAG GCAGAATTAC AAATTAAGTT GAATCCTGTG 
TTTTGTGAGC TGGATATCAG TATTGTGGAC AGGTTAAATT CCTTGCTCCA ACCACAAAAG 
CTTACCACAG TAGAGATGAT GGCATCTCAC ATGTATACTT CATACAATAA ACATATTAGT 
CTGCACAAGG CTTTCACTGA AGTATTTCTG GATGATTCAC ATAATCCTGC AAATTGTCGG 
ATATCCGTAC AAGTTGCCAC ACCAGCTTTA AACCTTTCTG TTCGCTTCCC AATACCTGAT 
CTTCGGTCAG ATCAAGAAAG AGGACCATGG TTTAAGAAGT CACTTCAGAA GGAGATGCTT 
CATTTAGCAT TCACAGACCT AGAATTTAAG ACTGAATTTA TAGGAGGATC AACCCCAGAG 
CAAATTAAAT TGGAACTTAC CTTTAGAGAA CTAGTTGGAT CATTCCAGGA AGATAGAGGA 
GAACCATCTA TTAAGTTTTT CCATGTGTCT AGTGGAGTAG ATGGAGATAC AGCATCATCC 
GATGACTTTG ACTGGCCACG AATTGTACTC AAATTAAATC CGCCAGCTGT GCATTCCATT 
TTGGAGCGAA TAGCTGCGGA GGAAGAAGAG AGTGATGGCC GCTACCAGGA AGAGGAGGAA 
GGGGGTGCTC ATTCCCTGAA GGATGTTTGT GATCTGCGAA GACCGGCCCC ATCTCCCTTC 
TCCTCTCGGA GAGTCATGTT TGAAAATGAG CAGATGGTGA TGCCTGGAGA CCCTGTAGAA 
ATGACAGAGT TTCAGGATAA AGCAATCAGC AATTCTCACT ATGTGCTGGA ACTTACGTTA 
CCCAACATTC ATATAACACT ACCTAATAAA AGCTTTTATG AGAAGCTTTA TAATAGGATC 
TTTAATGACT TGCTGCTGTG GGAACCAACA GCTCCTTCAC CAGTGGAGAC ATTTGAAAAT 
ATTTCTTATG GTCTTGGTCT TTCTGTAGCC AGTCAGCTGA TTAACACCTT CAACAAAGAG 
AGTTTTAGTC CATTTAAATC CACAGTTCAC TATGATGAGG AAAGTGGATC TGAGGAGGAG 
ACGTTGCAGT ATTTTTCTAC AGTGGATCCC AACTATCGTT CCCGTAGGAA AAAAAAATTA 
GACTCTCAGA ACAAGAACTC ACAGAGTTTT CTCTCTGTTC TTCTGAGTAT CAATCATGGA 
TTAATGGCAG TGTTTACAGA TGTCAGGCAA GATAATGGAG AGCTGTTGGA AAATAAGCAT 
GGCGAGTTCT GGTTAGAGTT CAACAGTGGT TCCTTGTTTT GTGTGACCAA GTATGAAGGC 
TGTGATGACA AGCACTACAT CTGCCTTCAT GCCAGCAGTC TCAGTCTATA CCACAAAGGC 
GTAGTAGATG GAGTGATTCT TCCTACAGAA ACACGACTGC CTAGTTCAAC CCGCCCACAC 
TGGTTGGAAC CTACTATTTA TTCCTCTGAA GAAGATGGCC TCAGTAGAGC TTCTTCAGAT 
GGTGTTGGAG GAGACACTTT GAATATGCTC TCTGTTGCAG TTAAAATACT TTCTGATAAA 
TCAGAGTCCA ATACAAAGGA ATTTCTCATT GCTGTGGGGT TGAAAGGAGC CACACTCCAG 
CACAGAATAC TTCCTTCTGG GCTTAGCTGG CACGAGCAGA TTTTATACTT CTTGAATATT 
GCTGATGAAC CTGTTCTGGG ATATAATCCT CCAACTTCGT TTACAACTTT TCATGTTCAT 
CTCTGGAGCT GCGCACTTGA TTACAGGCCT CTTTATTTGC CGATCCGATC TCTTCTCACT 
GTGGAAACCT TCAGCGTTTC AAGTAGCGTG GCCTTGGACA AATCCTCGTC TACTCTCAGG 
ATAATCTTGG ATGAAGCTGC TTTACATCTG TCTGACAAGT GTAATACTGT CACTATTAAT 
TTGAGTAGAG ATTACGTTCG TGTGATGGAT ATGGGGCTTT TGGAATTAAC CATAACTGCA 
ATGAAATCTG ATTCTGATGG AGAGCAAACT GAGCCACGCT TCGAGCTACA CTGTTCTAGT 
GACATTGTCC ATATCCGAAC GTGCTCAGAC TCTTGTGCTG CGTTAATGAA CCTCATTCAA 
TACATTGCAA GCTATGGGGA CTTACATGCA GCGAGCAAGG TAGAGATGAA GCCTGGAGCC 
CCTCACCGAA AGATGAAGGT AGATTCCAGT GGTCGTTCAT CCTCACGAGG TCCAGTCCTT 
CCTGAAGCCG ACCAGCAGAT GTTACGTGAT TTGATGAGTG ACGCTATGGA AGAGGTTGAT 
CTGCAACAGA CTGCTACGTC TACGCAAGCC CAGGCTAACG GTGTTTTGGA TGAAAAATCT 
CAAATTCAGG AGCCTTGTTG TTCAGACCTC TTCCTGTTTC CAGATGAGAG TGGGAATGTG 
TCCCAGGAGC CTGGCCCTCC TTATACCTCA TTCACACCCC ACTCGATTAG TGATGCAATG 
GCAGGTGTGC CTACTGAGAA TGATGACTTT TGCATTCTTT TTGCACCAAG AGCAAACAGT 
CAGGATAAGG AAGAAGAGCC CATTGTAAAA GTAATGGTTG ATGATGCAAT TGTAATAAGA 
GAGAATTACT TTAGTCAGCC TGTTAAGAAG ACCGATACAA GCAAAGCCCC ATTGCATTTT 
CCGGTCCCTG TCGTCCGCTA TGTTGTTAAA GAAGTCTCTC TTGTTTGGCA TCTTTATGGA 
GGAAAGGATT TTGGAACAGC CCCTCCTACT TCTCCAGCTA AAAGTTACAT CAGTCCTCAC 
AGCTCACCTT CTCACACACC CACAAGGCAT GGACGCAACA TGGTATGTGG GGGAAAAGGA 
AGAAACCATG ACTTTTTAAT GGAAATACAA TTAAGCAAGG TGAAGTTTCA GCACGAGGTC 
TACCCGCCTT GCAAGCCAGA GTGTGATTCT GGCCTCATAG AGCACCCAGT CTCCCGGCAG 
GTGTTCATTG TGCAGGATCT TGAAATTCGA GATCGTCTGG CAACATCACA AATGAATAAG 
TTTTTGTACC TGTATTGTAG TAAAGAAATG CCTCGAAAAG CTCATTCCAA CATGCTGACA 
GTGAAAGCAT TACATGTGCG ACCGGAGTCT GGCAGGTCAC CACAGGAATG CTGCTTGAGA 
GTATCCCTAA TGCCACTTCG CCTCAATATT GACCAGGATG CCTTGTTCTT CCTGAAGGAT 
TTCTTCACAA GTCTTTCTAC GGAAGTAGAG CTTCTAATGG CTCCAGATCC AGAAGTTAAA 
AAGTCTCCTG GAGCTGATGT TACCTGCAGT TTGCCAAGGC ACTTAAGCGC CTCCAAAGAG 
CCAAATCTAG TTCTGTCTTT CCCTGGGCCA AAGCAGCCTT CCCAAGATGA TAGTGCCAAT 
TCAGCGGAAG TGGTTAATGG GGAAGAAGAG AAGGACTTCT CAGCTGAAGA AACATCATTT 
AGTGATCAGC CCGTGTTTTT TAGAGAATTT AGATTCACAT CAGAAGTACC TATTCGACTT 
GATTATCATG GCAAACACGT ATCAATGGAT CAGGGTACAC TAGCTGGGAT TCTGATTGGT 
CTGGCCCAGT TAAACTGTTC AGAACTGAAG CTAAAGAGGC TTTTCTACCG GCATGGCTTG 
CTAGGTGTTG ACAAATTGTT TTCATATGCA ATCACCGAAT GGCTTACTGA CATTAAGAAG 
AATCAGCTAC CAGGAATCCT GGGAGGTGTG GGGCCTATGC ATTCACTAGT ACAATTAGTG 
CAAGGCCTAA AGGACTTGGT CTGGTTACCG ATAGAGCAAT ACCGGAAGGA CGGTCGTATT 
GTCAGAGGGT TCCAGAGAGG CGCTGCCTCC TTTGGCACCT CAACAGCGAT GGCTGCTCTT 
GAGCTCACAA ACAGAATGGT TCAGACTATA CAGGCAGCAG CAGAGACTGC TTATGATATG 
GTATCTCCTG GGACCCTTTC TATTGAGCCC AAGAAGATCA AAAGGTTTCC TCATCACCGC 
TTAGCCCACC AGCCAGTGGA CCTGCGAGAA GGTGTGGCCA AGGCCTACAG TGTTGTGAAA 
GAGGGACTTA CAGACACAGC TCAGACGATT TATGAAACTG CAGCACGAGA ACATGAGAGC 
AGAGGCGTCA CTGGTGCTGT GGGCGAGGTC CTGCGCCAGA TTCCTCCCGC AGTGGTGAAA 
CCTCTGATTG TGGCCACAGA AGCAACATCA AATGTATTAG GTGGCATGAG AAACCAGATT 
AGACCAGATG TTCGGCAAGA CGAGTCACAG AAATGGCGCC ATGGGGAAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ATG2B
Entrez Gene ID	480428
Full Name	autophagy related 2B
General protein information	Preferred Names autophagy related 2B Names autophagy-related protein 2 homolog B ATG2 autophagy related 2 homolog B
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Mus musculus (house mouse)	Atg2b	NP_083930.5
Canis lupus familiaris (dog)	ATG2B	XP_537549.3
Gallus gallus (chicken)	ATG2B	XP_421350.3
Drosophila melanogaster (fruit fly)	Atg2	NP_001261341.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004092	XP_312964.5
Xenopus tropicalis (tropical clawed frog)	atg2b	NP_001072626.1
Homo sapiens (human)	ATG2B	NP_060506.5
Bos taurus (cattle)	ATG2B	NP_001193682.1
Pan troglodytes (chimpanzee)	ATG2B	XP_510152.3
	ATG2B	XP_001101467.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ATG2B cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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