NIN gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022421994.1	XP_022277702.1	ninein isoform X6
XM_014115890.1	XP_013971365.1	ninein isoform X2
XM_005623386.1	XP_005623443.1	ninein isoform X3
XM_014115895.1	XP_013971370.1	ninein isoform X11
XM_537442.5	XP_537442.2	ninein isoform X4
XM_014115894.1	XP_013971369.1	ninein isoform X9
XM_022421991.1	XP_022277699.1	ninein isoform X3
XM_022421995.1	XP_022277703.1	ninein isoform X7
XM_022421996.1	XP_022277704.1	ninein isoform X8
XM_022421997.1	XP_022277705.1	ninein isoform X9
XM_014115891.1	XP_013971366.1	ninein isoform X5
XM_003435130.3	XP_003435178.1	ninein isoform X8
XM_022421990.1	XP_022277698.1	ninein isoform X2
XM_014115892.1	XP_013971367.1	ninein isoform X6
XM_005623390.2	XP_005623447.1	ninein isoform X10
XM_022421998.1	XP_022277706.1	ninein isoform X10
XM_014115888.1	XP_013971363.1	ninein isoform X1
XM_014115889.1	XP_013971364.1	ninein isoform X1
XM_014115893.1	XP_013971368.1	ninein isoform X7
XM_014115888.2	XP_013971363.1	ninein isoform X1
XM_022421992.1	XP_022277700.1	ninein isoform X4
XM_022421993.1	XP_022277701.1	ninein isoform X5
XM_022421999.1	XP_022277707.1	ninein isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe37892	XM_014115895.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe37892	XM_022421999.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCe37891	XM_022421997.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe37891	XM_014115894.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OCe10365	XM_005623390.2 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe10365	XM_022421998.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe290084	XM_022421994.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37886	XM_014115889.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37886	XM_014115888.2 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37887	XM_014115890.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37887	XM_022421990.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37888	XM_014115891.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37889	XM_014115892.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37889	XM_022421993.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37890	XM_022421995.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37890	XM_014115893.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe10360	XM_005623386.1 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe10360	XM_022421991.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe10361	XM_537442.5 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe10361	XM_022421992.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe10364	XM_022421996.1 Latest version!	Canis lupus familiaris ninein (NIN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe10364	XM_003435130.3 Latest version!	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe37886	XM_014115888.1	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

NIN ( XM_014115895.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971370.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe37892
Clone ID Related Accession (Same CDS sequence)	XM_014115895.1 , XM_022421999.1
Accession Version	XM_014115895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGATATGAA 
TCTGAGAAGC TTCAAGAAGA AAATTCTATT TTAAGAAATG AAATTACTAC TTTAAATGAA 
GAAGATAGCA TTTCTAACCT GAAATTAGGG AAATTAAATG GATCTCAGGA AGAAATGTGG 
CAAAAAATAG AAACTGTAAA ACAGGAAAAA GCGGCAGTTC AGAAGATGGC TGAAAATTTA 
AAGAAACAGA TTTCAGAATT AAAAACCAAA AACCAACAGT TGGATTTGGA AAATATAGAA 
CTTAGCCAAA AGAACTCTCA AAATGAGAAA GAACTGCAAG AACTTAATGA ACGTCTGGCA 
GAAATGCTAT GCCAGAAGGA TGAAGAGCCA GGACACAGTA CATTTGAGGA ATGGAAACAA 
GAAAAGTCTA ATCTGAAAGA AGAACTGGAA CAATGCAAAG CGCAGTCCTC AGCTTTAGTG 
TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT AAAATACAGA CTCATATGGT GGAACAGGAA 
AACCTCCTTC TCAAAGATGA ACTGGAGAAA ATGAAACAAC TGCACAGATG TCCTGATCTC 
ACTGACTTCC AGCAAAAACT TTCTAGTGTT CTAAGCTACA ATGAAAAACT GCTGAAAGAA 
AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC TGTGTGGATA AGTTGGCAAA ATCAAGTCTT 
TTAGAGCACA GAATAGCTAC TATGAAGCAA GAACAGAAGT CTTGGGAACA CCAGAGTGAA 
ACTTTAAAGT CCCAACTAGT GGCTTCACAG GAAAAGGTCC AGAATTTAGA AGACACCCTG 
CAGAATGTAA ACTTGCAAAT GTCGCTGATT AAATCTGACC TTCGAGTTAC TCAGCAGGAA 
AAGGAGGCTT TAAAACAAGA AGTGGTGTCT TTATATAAGC AACTTCAGAA TGCTGATGAC 
AAGAACTGGG CCCCAGAAAT AGTCAGCCAT CCATCAGGAT TCCATAACCA GCAGCAAAGG 
CTGACTTGGG ACAAGATGGA TCATCTGGTA AAGGAGGAAC AACAGCTGCT TTGGCAAGAG 
AATGAGAAAC TCCATACCGT GGTACGGAAC ACCAAAGCAG AGCTCACGCA CTCCCGGGAG 
AAGGTCCGTC AGTTGGAATC CAGCCTTATT CCTCCCAAGC ATCAGAAACA TCTAAACCCA 
TCAGCTACTA TGAAGCCCAC AGAGCAAGAG AAGTTGAGCT TAAAGAGAGA GTGTGAACAG 
TTTCCAAAGG AACAGCCTCC TACTAATAGG AAGGTCAGTC AGATGAATTC CCTTGAAAGA 
GAATTAGAAA CGATTCATTT GGAAAATGAA GGCCTGAAAA AGAAACAAGT AAAACTGGAT 
GAGCAGCTAA TGGAGATGCA GCACCTGAGG TCCACTGCGA TGCTTAGCCC GTCCCCTCAC 
GCTTGGGATC TCCAGCTGCG CCAGCAGCAA GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT 
CTGCAGCTTC AACAACAGCT GCTGCAGGCA GAGAGGATAA ACCAGTGCCT GCAGGAGGAA 
CTTGAAAACA GGACCTCCGA AACCAACACC CCGCAGGGAA ACCAGGAACA ACTAGTGACT 
GTCATGGAAG AACGAATGAT GGAAGTTGAA CAGAAGTTGA AACTGGTGAA GAGGCTTCTT 
CAGGAGAAAG TGAATCAGCT CAAAGAACAA CTCTGCAAGA ACACTAAAGC AGATGCAGTG 
GTGAAGGATT TGTATGTTGA AAATGCCCAG TTGTTGAAAG CTCTGGAAAT GACTGAACAG 
CGACAAAAAA CAGCAGAGAA GAAAAATTAC CTCCTGGAAG AGAAGATTGC CAGCCTCAGT 
AATATAGTCA GGAATCTGGC ACCAGCACCA CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971370.1
CDS	240..4499
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_014115895.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115895.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGATATGAA 
TCTGAGAAGC TTCAAGAAGA AAATTCTATT TTAAGAAATG AAATTACTAC TTTAAATGAA 
GAAGATAGCA TTTCTAACCT GAAATTAGGG AAATTAAATG GATCTCAGGA AGAAATGTGG 
CAAAAAATAG AAACTGTAAA ACAGGAAAAA GCGGCAGTTC AGAAGATGGC TGAAAATTTA 
AAGAAACAGA TTTCAGAATT AAAAACCAAA AACCAACAGT TGGATTTGGA AAATATAGAA 
CTTAGCCAAA AGAACTCTCA AAATGAGAAA GAACTGCAAG AACTTAATGA ACGTCTGGCA 
GAAATGCTAT GCCAGAAGGA TGAAGAGCCA GGACACAGTA CATTTGAGGA ATGGAAACAA 
GAAAAGTCTA ATCTGAAAGA AGAACTGGAA CAATGCAAAG CGCAGTCCTC AGCTTTAGTG 
TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT AAAATACAGA CTCATATGGT GGAACAGGAA 
AACCTCCTTC TCAAAGATGA ACTGGAGAAA ATGAAACAAC TGCACAGATG TCCTGATCTC 
ACTGACTTCC AGCAAAAACT TTCTAGTGTT CTAAGCTACA ATGAAAAACT GCTGAAAGAA 
AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC TGTGTGGATA AGTTGGCAAA ATCAAGTCTT 
TTAGAGCACA GAATAGCTAC TATGAAGCAA GAACAGAAGT CTTGGGAACA CCAGAGTGAA 
ACTTTAAAGT CCCAACTAGT GGCTTCACAG GAAAAGGTCC AGAATTTAGA AGACACCCTG 
CAGAATGTAA ACTTGCAAAT GTCGCTGATT AAATCTGACC TTCGAGTTAC TCAGCAGGAA 
AAGGAGGCTT TAAAACAAGA AGTGGTGTCT TTATATAAGC AACTTCAGAA TGCTGATGAC 
AAGAACTGGG CCCCAGAAAT AGTCAGCCAT CCATCAGGAT TCCATAACCA GCAGCAAAGG 
CTGACTTGGG ACAAGATGGA TCATCTGGTA AAGGAGGAAC AACAGCTGCT TTGGCAAGAG 
AATGAGAAAC TCCATACCGT GGTACGGAAC ACCAAAGCAG AGCTCACGCA CTCCCGGGAG 
AAGGTCCGTC AGTTGGAATC CAGCCTTATT CCTCCCAAGC ATCAGAAACA TCTAAACCCA 
TCAGCTACTA TGAAGCCCAC AGAGCAAGAG AAGTTGAGCT TAAAGAGAGA GTGTGAACAG 
TTTCCAAAGG AACAGCCTCC TACTAATAGG AAGGTCAGTC AGATGAATTC CCTTGAAAGA 
GAATTAGAAA CGATTCATTT GGAAAATGAA GGCCTGAAAA AGAAACAAGT AAAACTGGAT 
GAGCAGCTAA TGGAGATGCA GCACCTGAGG TCCACTGCGA TGCTTAGCCC GTCCCCTCAC 
GCTTGGGATC TCCAGCTGCG CCAGCAGCAA GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT 
CTGCAGCTTC AACAACAGCT GCTGCAGGCA GAGAGGATAA ACCAGTGCCT GCAGGAGGAA 
CTTGAAAACA GGACCTCCGA AACCAACACC CCGCAGGGAA ACCAGGAACA ACTAGTGACT 
GTCATGGAAG AACGAATGAT GGAAGTTGAA CAGAAGTTGA AACTGGTGAA GAGGCTTCTT 
CAGGAGAAAG TGAATCAGCT CAAAGAACAA CTCTGCAAGA ACACTAAAGC AGATGCAGTG 
GTGAAGGATT TGTATGTTGA AAATGCCCAG TTGTTGAAAG CTCTGGAAAT GACTGAACAG 
CGACAAAAAA CAGCAGAGAA GAAAAATTAC CTCCTGGAAG AGAAGATTGC CAGCCTCAGT 
AATATAGTCA GGAATCTGGC ACCAGCACCA CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421999.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277707.1 Canis lupus familiaris ninein (NIN), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe37892
Clone ID Related Accession (Same CDS sequence)	XM_014115895.1 , XM_022421999.1
Accession Version	XM_022421999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGATATGAA 
TCTGAGAAGC TTCAAGAAGA AAATTCTATT TTAAGAAATG AAATTACTAC TTTAAATGAA 
GAAGATAGCA TTTCTAACCT GAAATTAGGG AAATTAAATG GATCTCAGGA AGAAATGTGG 
CAAAAAATAG AAACTGTAAA ACAGGAAAAA GCGGCAGTTC AGAAGATGGC TGAAAATTTA 
AAGAAACAGA TTTCAGAATT AAAAACCAAA AACCAACAGT TGGATTTGGA AAATATAGAA 
CTTAGCCAAA AGAACTCTCA AAATGAGAAA GAACTGCAAG AACTTAATGA ACGTCTGGCA 
GAAATGCTAT GCCAGAAGGA TGAAGAGCCA GGACACAGTA CATTTGAGGA ATGGAAACAA 
GAAAAGTCTA ATCTGAAAGA AGAACTGGAA CAATGCAAAG CGCAGTCCTC AGCTTTAGTG 
TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT AAAATACAGA CTCATATGGT GGAACAGGAA 
AACCTCCTTC TCAAAGATGA ACTGGAGAAA ATGAAACAAC TGCACAGATG TCCTGATCTC 
ACTGACTTCC AGCAAAAACT TTCTAGTGTT CTAAGCTACA ATGAAAAACT GCTGAAAGAA 
AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC TGTGTGGATA AGTTGGCAAA ATCAAGTCTT 
TTAGAGCACA GAATAGCTAC TATGAAGCAA GAACAGAAGT CTTGGGAACA CCAGAGTGAA 
ACTTTAAAGT CCCAACTAGT GGCTTCACAG GAAAAGGTCC AGAATTTAGA AGACACCCTG 
CAGAATGTAA ACTTGCAAAT GTCGCTGATT AAATCTGACC TTCGAGTTAC TCAGCAGGAA 
AAGGAGGCTT TAAAACAAGA AGTGGTGTCT TTATATAAGC AACTTCAGAA TGCTGATGAC 
AAGAACTGGG CCCCAGAAAT AGTCAGCCAT CCATCAGGAT TCCATAACCA GCAGCAAAGG 
CTGACTTGGG ACAAGATGGA TCATCTGGTA AAGGAGGAAC AACAGCTGCT TTGGCAAGAG 
AATGAGAAAC TCCATACCGT GGTACGGAAC ACCAAAGCAG AGCTCACGCA CTCCCGGGAG 
AAGGTCCGTC AGTTGGAATC CAGCCTTATT CCTCCCAAGC ATCAGAAACA TCTAAACCCA 
TCAGCTACTA TGAAGCCCAC AGAGCAAGAG AAGTTGAGCT TAAAGAGAGA GTGTGAACAG 
TTTCCAAAGG AACAGCCTCC TACTAATAGG AAGGTCAGTC AGATGAATTC CCTTGAAAGA 
GAATTAGAAA CGATTCATTT GGAAAATGAA GGCCTGAAAA AGAAACAAGT AAAACTGGAT 
GAGCAGCTAA TGGAGATGCA GCACCTGAGG TCCACTGCGA TGCTTAGCCC GTCCCCTCAC 
GCTTGGGATC TCCAGCTGCG CCAGCAGCAA GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT 
CTGCAGCTTC AACAACAGCT GCTGCAGGCA GAGAGGATAA ACCAGTGCCT GCAGGAGGAA 
CTTGAAAACA GGACCTCCGA AACCAACACC CCGCAGGGAA ACCAGGAACA ACTAGTGACT 
GTCATGGAAG AACGAATGAT GGAAGTTGAA CAGAAGTTGA AACTGGTGAA GAGGCTTCTT 
CAGGAGAAAG TGAATCAGCT CAAAGAACAA CTCTGCAAGA ACACTAAAGC AGATGCAGTG 
GTGAAGGATT TGTATGTTGA AAATGCCCAG TTGTTGAAAG CTCTGGAAAT GACTGAACAG 
CGACAAAAAA CAGCAGAGAA GAAAAATTAC CTCCTGGAAG AGAAGATTGC CAGCCTCAGT 
AATATAGTCA GGAATCTGGC ACCAGCACCA CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277707.1
CDS	184..4443
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_022421999.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421999.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGATATGAA 
TCTGAGAAGC TTCAAGAAGA AAATTCTATT TTAAGAAATG AAATTACTAC TTTAAATGAA 
GAAGATAGCA TTTCTAACCT GAAATTAGGG AAATTAAATG GATCTCAGGA AGAAATGTGG 
CAAAAAATAG AAACTGTAAA ACAGGAAAAA GCGGCAGTTC AGAAGATGGC TGAAAATTTA 
AAGAAACAGA TTTCAGAATT AAAAACCAAA AACCAACAGT TGGATTTGGA AAATATAGAA 
CTTAGCCAAA AGAACTCTCA AAATGAGAAA GAACTGCAAG AACTTAATGA ACGTCTGGCA 
GAAATGCTAT GCCAGAAGGA TGAAGAGCCA GGACACAGTA CATTTGAGGA ATGGAAACAA 
GAAAAGTCTA ATCTGAAAGA AGAACTGGAA CAATGCAAAG CGCAGTCCTC AGCTTTAGTG 
TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT AAAATACAGA CTCATATGGT GGAACAGGAA 
AACCTCCTTC TCAAAGATGA ACTGGAGAAA ATGAAACAAC TGCACAGATG TCCTGATCTC 
ACTGACTTCC AGCAAAAACT TTCTAGTGTT CTAAGCTACA ATGAAAAACT GCTGAAAGAA 
AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC TGTGTGGATA AGTTGGCAAA ATCAAGTCTT 
TTAGAGCACA GAATAGCTAC TATGAAGCAA GAACAGAAGT CTTGGGAACA CCAGAGTGAA 
ACTTTAAAGT CCCAACTAGT GGCTTCACAG GAAAAGGTCC AGAATTTAGA AGACACCCTG 
CAGAATGTAA ACTTGCAAAT GTCGCTGATT AAATCTGACC TTCGAGTTAC TCAGCAGGAA 
AAGGAGGCTT TAAAACAAGA AGTGGTGTCT TTATATAAGC AACTTCAGAA TGCTGATGAC 
AAGAACTGGG CCCCAGAAAT AGTCAGCCAT CCATCAGGAT TCCATAACCA GCAGCAAAGG 
CTGACTTGGG ACAAGATGGA TCATCTGGTA AAGGAGGAAC AACAGCTGCT TTGGCAAGAG 
AATGAGAAAC TCCATACCGT GGTACGGAAC ACCAAAGCAG AGCTCACGCA CTCCCGGGAG 
AAGGTCCGTC AGTTGGAATC CAGCCTTATT CCTCCCAAGC ATCAGAAACA TCTAAACCCA 
TCAGCTACTA TGAAGCCCAC AGAGCAAGAG AAGTTGAGCT TAAAGAGAGA GTGTGAACAG 
TTTCCAAAGG AACAGCCTCC TACTAATAGG AAGGTCAGTC AGATGAATTC CCTTGAAAGA 
GAATTAGAAA CGATTCATTT GGAAAATGAA GGCCTGAAAA AGAAACAAGT AAAACTGGAT 
GAGCAGCTAA TGGAGATGCA GCACCTGAGG TCCACTGCGA TGCTTAGCCC GTCCCCTCAC 
GCTTGGGATC TCCAGCTGCG CCAGCAGCAA GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT 
CTGCAGCTTC AACAACAGCT GCTGCAGGCA GAGAGGATAA ACCAGTGCCT GCAGGAGGAA 
CTTGAAAACA GGACCTCCGA AACCAACACC CCGCAGGGAA ACCAGGAACA ACTAGTGACT 
GTCATGGAAG AACGAATGAT GGAAGTTGAA CAGAAGTTGA AACTGGTGAA GAGGCTTCTT 
CAGGAGAAAG TGAATCAGCT CAAAGAACAA CTCTGCAAGA ACACTAAAGC AGATGCAGTG 
GTGAAGGATT TGTATGTTGA AAATGCCCAG TTGTTGAAAG CTCTGGAAAT GACTGAACAG 
CGACAAAAAA CAGCAGAGAA GAAAAATTAC CTCCTGGAAG AGAAGATTGC CAGCCTCAGT 
AATATAGTCA GGAATCTGGC ACCAGCACCA CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115894.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971369.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe37891
Clone ID Related Accession (Same CDS sequence)	XM_014115894.1 , XM_022421997.1
Accession Version	XM_014115894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
AACTCTGTGG TTGGAAGTAG CAGAGAAGGG TGCAGTTCTC TGCCTGAGAT AGTGTGTGAA 
GATGCAGCAC CTGAGGTCCA CTGCGATGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971369.1
CDS	242..6214
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMENSVVGSSREGCSSLPEIVCEDAAPEVHCDA

Target ORF information:

RefSeq Version	XM_014115894.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115894.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
AACTCTGTGG TTGGAAGTAG CAGAGAAGGG TGCAGTTCTC TGCCTGAGAT AGTGTGTGAA 
GATGCAGCAC CTGAGGTCCA CTGCGATGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421997.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277705.1 Canis lupus familiaris ninein (NIN), transcript variant X11, mRNA.

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CloneID	OCe37891
Clone ID Related Accession (Same CDS sequence)	XM_014115894.1 , XM_022421997.1
Accession Version	XM_022421997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
AACTCTGTGG TTGGAAGTAG CAGAGAAGGG TGCAGTTCTC TGCCTGAGAT AGTGTGTGAA 
GATGCAGCAC CTGAGGTCCA CTGCGATGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277705.1
CDS	197..6169
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMENSVVGSSREGCSSLPEIVCEDAAPEVHCDA

Target ORF information:

RefSeq Version	XM_022421997.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421997.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
AACTCTGTGG TTGGAAGTAG CAGAGAAGGG TGCAGTTCTC TGCCTGAGAT AGTGTGTGAA 
GATGCAGCAC CTGAGGTCCA CTGCGATGCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_005623390.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623447.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X11, mRNA.

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CloneID	OCe10365
Clone ID Related Accession (Same CDS sequence)	XM_005623390.2 , XM_022421998.1
Accession Version	XM_005623390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGAACTC TGTGGTTGGA 
AGTAGCAGAG AAGGGTGCAG TTCTCTGCCT GAGATAGTGT GTGAAGATGC AGCACCTGAG 
GTCCACTGCG ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623447.1
CDS	242..6199
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMENSVVGSSREGCSSLPEIVCEDAAPEVHCDA

Target ORF information:

RefSeq Version	XM_005623390.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623390.2

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGAACTC TGTGGTTGGA 
AGTAGCAGAG AAGGGTGCAG TTCTCTGCCT GAGATAGTGT GTGAAGATGC AGCACCTGAG 
GTCCACTGCG ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421998.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277706.1 Canis lupus familiaris ninein (NIN), transcript variant X12, mRNA.

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CloneID	OCe10365
Clone ID Related Accession (Same CDS sequence)	XM_005623390.2 , XM_022421998.1
Accession Version	XM_022421998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGAACTC TGTGGTTGGA 
AGTAGCAGAG AAGGGTGCAG TTCTCTGCCT GAGATAGTGT GTGAAGATGC AGCACCTGAG 
GTCCACTGCG ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277706.1
CDS	196..6153
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMENSVVGSSREGCSSLPEIVCEDAAPEVHCDA

Target ORF information:

RefSeq Version	XM_022421998.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421998.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGAACTC TGTGGTTGGA 
AGTAGCAGAG AAGGGTGCAG TTCTCTGCCT GAGATAGTGT GTGAAGATGC AGCACCTGAG 
GTCCACTGCG ATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421994.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277702.1 Canis lupus familiaris ninein (NIN), transcript variant X8, mRNA.

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CloneID	OCe290084
Clone ID Related Accession (Same CDS sequence)	XM_022421994.1
Accession Version	XM_022421994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGGTCAG TCAGATGAAT TCCCTTGAAA GAGAATTAGA AACGATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAGCAGCT AATGGAGGGA 
AACCAGGAAC AACTAGTGAC TGTCATGGAA GAACGAATGA TGGAAGTTGA ACAGAAGTTG 
AAACTGGTGA AGAGGCTTCT TCAGGAGAAA GTGAATCAGC TCAAAGAACA ACTCTGCAAG 
AACACTAAAG CAGATGCAGT GGTGAAGGAT TTGTATGTTG AAAATGCCCA GTTGTTGAAA 
GCTCTGGAAA TGACTGAACA GCGACAAAAA ACAGCAGAGA AGAAAAATTA CCTCCTGGAA 
GAGAAGATTG CCAGCCTCAG TAATATAGTC AGGAATCTGG CACCAGCACC ACTGACGTCT 
ATACCTCCCT TGAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277702.1
CDS	197..6397
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_022421994.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421994.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGGTCAG TCAGATGAAT TCCCTTGAAA GAGAATTAGA AACGATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAGCAGCT AATGGAGGGA 
AACCAGGAAC AACTAGTGAC TGTCATGGAA GAACGAATGA TGGAAGTTGA ACAGAAGTTG 
AAACTGGTGA AGAGGCTTCT TCAGGAGAAA GTGAATCAGC TCAAAGAACA ACTCTGCAAG 
AACACTAAAG CAGATGCAGT GGTGAAGGAT TTGTATGTTG AAAATGCCCA GTTGTTGAAA 
GCTCTGGAAA TGACTGAACA GCGACAAAAA ACAGCAGAGA AGAAAAATTA CCTCCTGGAA 
GAGAAGATTG CCAGCCTCAG TAATATAGTC AGGAATCTGG CACCAGCACC ACTGACGTCT 
ATACCTCCCT TGAGGTCATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115888.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971363.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X1, mRNA.

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CloneID	OCe37886
Clone ID Related Accession (Same CDS sequence)	XM_014115888.1 , XM_014115889.1 , XM_014115888.2
Accession Version	XM_014115888.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTCTG CAAGAACACT AAAGCAGATG CAGTGGTGAA GGATTTGTAT 
GTTGAAAATG CCCAGTTGTT GAAAGCTCTG GAAATGACTG AACAGCGACA AAAAACAGCA 
GAGAAGAAAA ATTACCTCCT GGAAGAGAAG ATTGCCAGCC TCAGTAATAT AGTCAGGAAT 
CTGGCACCAG CACCACTGAC GTCTATACCT CCCTTGAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971363.1
CDS	242..6646
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_014115888.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115888.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTCTG CAAGAACACT AAAGCAGATG CAGTGGTGAA GGATTTGTAT 
GTTGAAAATG CCCAGTTGTT GAAAGCTCTG GAAATGACTG AACAGCGACA AAAAACAGCA 
GAGAAGAAAA ATTACCTCCT GGAAGAGAAG ATTGCCAGCC TCAGTAATAT AGTCAGGAAT 
CTGGCACCAG CACCACTGAC GTCTATACCT CCCTTGAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115889.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971364.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X2, mRNA.

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CloneID	OCe37886
Clone ID Related Accession (Same CDS sequence)	XM_014115888.1 , XM_014115889.1 , XM_014115888.2
Accession Version	XM_014115889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTCTG CAAGAACACT AAAGCAGATG CAGTGGTGAA GGATTTGTAT 
GTTGAAAATG CCCAGTTGTT GAAAGCTCTG GAAATGACTG AACAGCGACA AAAAACAGCA 
GAGAAGAAAA ATTACCTCCT GGAAGAGAAG ATTGCCAGCC TCAGTAATAT AGTCAGGAAT 
CTGGCACCAG CACCACTGAC GTCTATACCT CCCTTGAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971364.1
CDS	314..6718
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_014115889.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115889.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTCTG CAAGAACACT AAAGCAGATG CAGTGGTGAA GGATTTGTAT 
GTTGAAAATG CCCAGTTGTT GAAAGCTCTG GAAATGACTG AACAGCGACA AAAAACAGCA 
GAGAAGAAAA ATTACCTCCT GGAAGAGAAG ATTGCCAGCC TCAGTAATAT AGTCAGGAAT 
CTGGCACCAG CACCACTGAC GTCTATACCT CCCTTGAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115888.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971363.1 Canis lupus familiaris ninein (NIN), transcript variant X1, mRNA.

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CloneID	OCe37886
Clone ID Related Accession (Same CDS sequence)	XM_014115888.1 , XM_014115889.1 , XM_014115888.2
Accession Version	XM_014115888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115888.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTCTG CAAGAACACT AAAGCAGATG CAGTGGTGAA GGATTTGTAT 
GTTGAAAATG CCCAGTTGTT GAAAGCTCTG GAAATGACTG AACAGCGACA AAAAACAGCA 
GAGAAGAAAA ATTACCTCCT GGAAGAGAAG ATTGCCAGCC TCAGTAATAT AGTCAGGAAT 
CTGGCACCAG CACCACTGAC GTCTATACCT CCCTTGAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971363.1
CDS	177..6581
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_014115888.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115888.2

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTCTG CAAGAACACT AAAGCAGATG CAGTGGTGAA GGATTTGTAT 
GTTGAAAATG CCCAGTTGTT GAAAGCTCTG GAAATGACTG AACAGCGACA AAAAACAGCA 
GAGAAGAAAA ATTACCTCCT GGAAGAGAAG ATTGCCAGCC TCAGTAATAT AGTCAGGAAT 
CTGGCACCAG CACCACTGAC GTCTATACCT CCCTTGAGGT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115890.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971365.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X3, mRNA.

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CloneID	OCe37887
Clone ID Related Accession (Same CDS sequence)	XM_014115890.1 , XM_022421990.1
Accession Version	XM_014115890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGGTCAG TCAGATGAAT TCCCTTGAAA GAGAATTAGA AACGATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAGCAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGC GATGCTTAGC CCGTCCCCTC ACGCTTGGGA TCTCCAGCTG 
CGCCAGCAGC AAGCCTGTCC CACGGTGCCC AGGGAGCAGT TTCTGCAGCT TCAACAACAG 
CTGCTGCAGG CAGAGAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA CAGGACCTCC 
GAAACCAACA CCCCGCAGGG AAACCAGGAA CAACTAGTGA CTGTCATGGA AGAACGAATG 
ATGGAAGTTG AACAGAAGTT GAAACTGGTG AAGAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAACACTAAA GCAGATGCAG TGGTGAAGGA TTTGTATGTT 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTGGA AGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
GCACCAGCAC CACTGACGTC TATACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971365.1
CDS	242..6643
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_014115890.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115890.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGGTCAG TCAGATGAAT TCCCTTGAAA GAGAATTAGA AACGATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAGCAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGC GATGCTTAGC CCGTCCCCTC ACGCTTGGGA TCTCCAGCTG 
CGCCAGCAGC AAGCCTGTCC CACGGTGCCC AGGGAGCAGT TTCTGCAGCT TCAACAACAG 
CTGCTGCAGG CAGAGAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA CAGGACCTCC 
GAAACCAACA CCCCGCAGGG AAACCAGGAA CAACTAGTGA CTGTCATGGA AGAACGAATG 
ATGGAAGTTG AACAGAAGTT GAAACTGGTG AAGAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAACACTAAA GCAGATGCAG TGGTGAAGGA TTTGTATGTT 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTGGA AGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
GCACCAGCAC CACTGACGTC TATACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421990.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277698.1 Canis lupus familiaris ninein (NIN), transcript variant X2, mRNA.

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CloneID	OCe37887
Clone ID Related Accession (Same CDS sequence)	XM_014115890.1 , XM_022421990.1
Accession Version	XM_022421990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGGTCAG TCAGATGAAT TCCCTTGAAA GAGAATTAGA AACGATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAGCAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGC GATGCTTAGC CCGTCCCCTC ACGCTTGGGA TCTCCAGCTG 
CGCCAGCAGC AAGCCTGTCC CACGGTGCCC AGGGAGCAGT TTCTGCAGCT TCAACAACAG 
CTGCTGCAGG CAGAGAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA CAGGACCTCC 
GAAACCAACA CCCCGCAGGG AAACCAGGAA CAACTAGTGA CTGTCATGGA AGAACGAATG 
ATGGAAGTTG AACAGAAGTT GAAACTGGTG AAGAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAACACTAAA GCAGATGCAG TGGTGAAGGA TTTGTATGTT 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTGGA AGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
GCACCAGCAC CACTGACGTC TATACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277698.1
CDS	196..6597
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_022421990.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421990.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGGTCAG TCAGATGAAT TCCCTTGAAA GAGAATTAGA AACGATTCAT 
TTGGAAAATG AAGGCCTGAA AAAGAAACAA GTAAAACTGG ATGAGCAGCT AATGGAGATG 
CAGCACCTGA GGTCCACTGC GATGCTTAGC CCGTCCCCTC ACGCTTGGGA TCTCCAGCTG 
CGCCAGCAGC AAGCCTGTCC CACGGTGCCC AGGGAGCAGT TTCTGCAGCT TCAACAACAG 
CTGCTGCAGG CAGAGAGGAT AAACCAGTGC CTGCAGGAGG AACTTGAAAA CAGGACCTCC 
GAAACCAACA CCCCGCAGGG AAACCAGGAA CAACTAGTGA CTGTCATGGA AGAACGAATG 
ATGGAAGTTG AACAGAAGTT GAAACTGGTG AAGAGGCTTC TTCAGGAGAA AGTGAATCAG 
CTCAAAGAAC AACTCTGCAA GAACACTAAA GCAGATGCAG TGGTGAAGGA TTTGTATGTT 
GAAAATGCCC AGTTGTTGAA AGCTCTGGAA ATGACTGAAC AGCGACAAAA AACAGCAGAG 
AAGAAAAATT ACCTCCTGGA AGAGAAGATT GCCAGCCTCA GTAATATAGT CAGGAATCTG 
GCACCAGCAC CACTGACGTC TATACCTCCC TTGAGGTCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115891.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971366.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X6, mRNA.

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CloneID	OCe37888
Clone ID Related Accession (Same CDS sequence)	XM_014115891.1
Accession Version	XM_014115891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTACT ATGTGCCAAG TCCTGTTCTG AGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971366.1
CDS	242..6469
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLLCAKSCSEH

Target ORF information:

RefSeq Version	XM_014115891.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115891.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGGAAACCAG GAACAACTAG TGACTGTCAT GGAAGAACGA 
ATGATGGAAG TTGAACAGAA GTTGAAACTG GTGAAGAGGC TTCTTCAGGA GAAAGTGAAT 
CAGCTCAAAG AACAACTACT ATGTGCCAAG TCCTGTTCTG AGCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115892.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971367.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X7, mRNA.

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CloneID	OCe37889
Clone ID Related Accession (Same CDS sequence)	XM_014115892.1 , XM_022421993.1
Accession Version	XM_014115892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
GGAAACCAGG AACAACTAGT GACTGTCATG GAAGAACGAA TGATGGAAGT TGAACAGAAG 
TTGAAACTGG TGAAGAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAACACTA AAGCAGATGC AGTGGTGAAG GATTTGTATG TTGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTG 
GAAGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGGCACCAGC ACCACTGACG 
TCTATACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971367.1
CDS	242..6445
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_014115892.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115892.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
GGAAACCAGG AACAACTAGT GACTGTCATG GAAGAACGAA TGATGGAAGT TGAACAGAAG 
TTGAAACTGG TGAAGAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAACACTA AAGCAGATGC AGTGGTGAAG GATTTGTATG TTGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTG 
GAAGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGGCACCAGC ACCACTGACG 
TCTATACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421993.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277701.1 Canis lupus familiaris ninein (NIN), transcript variant X7, mRNA.

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CloneID	OCe37889
Clone ID Related Accession (Same CDS sequence)	XM_014115892.1 , XM_022421993.1
Accession Version	XM_022421993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
GGAAACCAGG AACAACTAGT GACTGTCATG GAAGAACGAA TGATGGAAGT TGAACAGAAG 
TTGAAACTGG TGAAGAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAACACTA AAGCAGATGC AGTGGTGAAG GATTTGTATG TTGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTG 
GAAGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGGCACCAGC ACCACTGACG 
TCTATACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277701.1
CDS	197..6400
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_022421993.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421993.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
GGAAACCAGG AACAACTAGT GACTGTCATG GAAGAACGAA TGATGGAAGT TGAACAGAAG 
TTGAAACTGG TGAAGAGGCT TCTTCAGGAG AAAGTGAATC AGCTCAAAGA ACAACTCTGC 
AAGAACACTA AAGCAGATGC AGTGGTGAAG GATTTGTATG TTGAAAATGC CCAGTTGTTG 
AAAGCTCTGG AAATGACTGA ACAGCGACAA AAAACAGCAG AGAAGAAAAA TTACCTCCTG 
GAAGAGAAGA TTGCCAGCCT CAGTAATATA GTCAGGAATC TGGCACCAGC ACCACTGACG 
TCTATACCTC CCTTGAGGTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421995.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277703.1 Canis lupus familiaris ninein (NIN), transcript variant X9, mRNA.

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CloneID	OCe37890
Clone ID Related Accession (Same CDS sequence)	XM_022421995.1 , XM_014115893.1
Accession Version	XM_022421995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGCCTTGCTA CCGGAGCCGC GAGCAGTGCA TGCAGATTCC 
TACAGAAGGA TTGGGCACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277703.1
CDS	197..6340
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQALLPEPRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_022421995.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421995.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGCCTTGCTA CCGGAGCCGC GAGCAGTGCA TGCAGATTCC 
TACAGAAGGA TTGGGCACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_014115893.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971368.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X8, mRNA.

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CloneID	OCe37890
Clone ID Related Accession (Same CDS sequence)	XM_022421995.1 , XM_014115893.1
Accession Version	XM_014115893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGCCTTGCTA CCGGAGCCGC GAGCAGTGCA TGCAGATTCC 
TACAGAAGGA TTGGGCACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971368.1
CDS	242..6385
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKFTLKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQALLPEPRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_014115893.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115893.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGTTTACTC TTAAGAACTG GGCCCCAGAA 
ATAGTCAGCC ATCCATCAGG ATTCCATAAC CAGCAGCAAA GGCTGACTTG GGACAAGATG 
GATCATCTGG TAAAGGAGGA ACAACAGCTG CTTTGGCAAG AGAATGAGAA ACTCCATACC 
GTGGTACGGA ACACCAAAGC AGAGCTCACG CACTCCCGGG AGAAGGTCCG TCAGTTGGAA 
TCCAGCCTTA TTCCTCCCAA GCATCAGAAA CATCTAAACC CATCAGCTAC TATGAAGCCC 
ACAGAGCAAG AGAAGTTGAG CTTAAAGAGA GAGTGTGAAC AGTTTCCAAA GGAACAGCCT 
CCTACTAATA GGAAGCAGGT CAGTCAGATG AATTCCCTTG AAAGAGAATT AGAAACGATT 
CATTTGGAAA ATGAAGGCCT GAAAAAGAAA CAAGTAAAAC TGGATGAGCA GCTAATGGAG 
ATGCAGCACC TGAGGTCCAC TGCGATGCTT AGCCCGTCCC CTCACGCTTG GGATCTCCAG 
CTGCGCCAGC AGCAAGCCTG TCCCACGGTG CCCAGGGAGC AGTTTCTGCA GCTTCAACAA 
CAGCTGCTGC AGGCAGAGAG GATAAACCAG TGCCTGCAGG AGGAACTTGA AAACAGGACC 
TCCGAAACCA ACACCCCGCA GGCCTTGCTA CCGGAGCCGC GAGCAGTGCA TGCAGATTCC 
TACAGAAGGA TTGGGCACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_005623386.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623443.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X4, mRNA.

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CloneID	OCe10360
Clone ID Related Accession (Same CDS sequence)	XM_005623386.1 , XM_022421991.1
Accession Version	XM_005623386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGCAGGTCA GTCAGATGAA TTCCCTTGAA AGAGAATTAG AAACGATTCA TTTGGAAAAT 
GAAGGCCTGA AAAAGAAACA AGTAAAACTG GATGAGCAGC TAATGGAGAT GCAGCACCTG 
AGGTCCACTG CGATGCTTAG CCCGTCCCCT CACGCTTGGG ATCTCCAGCT GCGCCAGCAG 
CAAGCCTGTC CCACGGTGCC CAGGGAGCAG TTTCTGCAGC TTCAACAACA GCTGCTGCAG 
GCAGAGAGGA TAAACCAGTG CCTGCAGGAG GAACTTGAAA ACAGGACCTC CGAAACCAAC 
ACCCCGCAGG GAAACCAGGA ACAACTAGTG ACTGTCATGG AAGAACGAAT GATGGAAGTT 
GAACAGAAGT TGAAACTGGT GAAGAGGCTT CTTCAGGAGA AAGTGAATCA GCTCAAAGAA 
CAACTCTGCA AGAACACTAA AGCAGATGCA GTGGTGAAGG ATTTGTATGT TGAAAATGCC 
CAGTTGTTGA AAGCTCTGGA AATGACTGAA CAGCGACAAA AAACAGCAGA GAAGAAAAAT 
TACCTCCTGG AAGAGAAGAT TGCCAGCCTC AGTAATATAG TCAGGAATCT GGCACCAGCA 
CCACTGACGT CTATACCTCC CTTGAGGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623443.1
CDS	242..6634
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_005623386.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623386.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGCAGGTCA GTCAGATGAA TTCCCTTGAA AGAGAATTAG AAACGATTCA TTTGGAAAAT 
GAAGGCCTGA AAAAGAAACA AGTAAAACTG GATGAGCAGC TAATGGAGAT GCAGCACCTG 
AGGTCCACTG CGATGCTTAG CCCGTCCCCT CACGCTTGGG ATCTCCAGCT GCGCCAGCAG 
CAAGCCTGTC CCACGGTGCC CAGGGAGCAG TTTCTGCAGC TTCAACAACA GCTGCTGCAG 
GCAGAGAGGA TAAACCAGTG CCTGCAGGAG GAACTTGAAA ACAGGACCTC CGAAACCAAC 
ACCCCGCAGG GAAACCAGGA ACAACTAGTG ACTGTCATGG AAGAACGAAT GATGGAAGTT 
GAACAGAAGT TGAAACTGGT GAAGAGGCTT CTTCAGGAGA AAGTGAATCA GCTCAAAGAA 
CAACTCTGCA AGAACACTAA AGCAGATGCA GTGGTGAAGG ATTTGTATGT TGAAAATGCC 
CAGTTGTTGA AAGCTCTGGA AATGACTGAA CAGCGACAAA AAACAGCAGA GAAGAAAAAT 
TACCTCCTGG AAGAGAAGAT TGCCAGCCTC AGTAATATAG TCAGGAATCT GGCACCAGCA 
CCACTGACGT CTATACCTCC CTTGAGGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421991.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277699.1 Canis lupus familiaris ninein (NIN), transcript variant X3, mRNA.

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CloneID	OCe10360
Clone ID Related Accession (Same CDS sequence)	XM_005623386.1 , XM_022421991.1
Accession Version	XM_022421991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGCAGGTCA GTCAGATGAA TTCCCTTGAA AGAGAATTAG AAACGATTCA TTTGGAAAAT 
GAAGGCCTGA AAAAGAAACA AGTAAAACTG GATGAGCAGC TAATGGAGAT GCAGCACCTG 
AGGTCCACTG CGATGCTTAG CCCGTCCCCT CACGCTTGGG ATCTCCAGCT GCGCCAGCAG 
CAAGCCTGTC CCACGGTGCC CAGGGAGCAG TTTCTGCAGC TTCAACAACA GCTGCTGCAG 
GCAGAGAGGA TAAACCAGTG CCTGCAGGAG GAACTTGAAA ACAGGACCTC CGAAACCAAC 
ACCCCGCAGG GAAACCAGGA ACAACTAGTG ACTGTCATGG AAGAACGAAT GATGGAAGTT 
GAACAGAAGT TGAAACTGGT GAAGAGGCTT CTTCAGGAGA AAGTGAATCA GCTCAAAGAA 
CAACTCTGCA AGAACACTAA AGCAGATGCA GTGGTGAAGG ATTTGTATGT TGAAAATGCC 
CAGTTGTTGA AAGCTCTGGA AATGACTGAA CAGCGACAAA AAACAGCAGA GAAGAAAAAT 
TACCTCCTGG AAGAGAAGAT TGCCAGCCTC AGTAATATAG TCAGGAATCT GGCACCAGCA 
CCACTGACGT CTATACCTCC CTTGAGGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277699.1
CDS	194..6586
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKQVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_022421991.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421991.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGCAGGTCA GTCAGATGAA TTCCCTTGAA AGAGAATTAG AAACGATTCA TTTGGAAAAT 
GAAGGCCTGA AAAAGAAACA AGTAAAACTG GATGAGCAGC TAATGGAGAT GCAGCACCTG 
AGGTCCACTG CGATGCTTAG CCCGTCCCCT CACGCTTGGG ATCTCCAGCT GCGCCAGCAG 
CAAGCCTGTC CCACGGTGCC CAGGGAGCAG TTTCTGCAGC TTCAACAACA GCTGCTGCAG 
GCAGAGAGGA TAAACCAGTG CCTGCAGGAG GAACTTGAAA ACAGGACCTC CGAAACCAAC 
ACCCCGCAGG GAAACCAGGA ACAACTAGTG ACTGTCATGG AAGAACGAAT GATGGAAGTT 
GAACAGAAGT TGAAACTGGT GAAGAGGCTT CTTCAGGAGA AAGTGAATCA GCTCAAAGAA 
CAACTCTGCA AGAACACTAA AGCAGATGCA GTGGTGAAGG ATTTGTATGT TGAAAATGCC 
CAGTTGTTGA AAGCTCTGGA AATGACTGAA CAGCGACAAA AAACAGCAGA GAAGAAAAAT 
TACCTCCTGG AAGAGAAGAT TGCCAGCCTC AGTAATATAG TCAGGAATCT GGCACCAGCA 
CCACTGACGT CTATACCTCC CTTGAGGTCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_537442.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537442.2 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X5, mRNA.

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CloneID	OCe10361
Clone ID Related Accession (Same CDS sequence)	XM_537442.5 , XM_022421992.1
Accession Version	XM_537442.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537442.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGGAA ACCAGGAACA ACTAGTGACT GTCATGGAAG AACGAATGAT GGAAGTTGAA 
CAGAAGTTGA AACTGGTGAA GAGGCTTCTT CAGGAGAAAG TGAATCAGCT CAAAGAACAA 
CTCTGCAAGA ACACTAAAGC AGATGCAGTG GTGAAGGATT TGTATGTTGA AAATGCCCAG 
TTGTTGAAAG CTCTGGAAAT GACTGAACAG CGACAAAAAA CAGCAGAGAA GAAAAATTAC 
CTCCTGGAAG AGAAGATTGC CAGCCTCAGT AATATAGTCA GGAATCTGGC ACCAGCACCA 
CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537442.2
CDS	242..6631
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_537442.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537442.5

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGGAA ACCAGGAACA ACTAGTGACT GTCATGGAAG AACGAATGAT GGAAGTTGAA 
CAGAAGTTGA AACTGGTGAA GAGGCTTCTT CAGGAGAAAG TGAATCAGCT CAAAGAACAA 
CTCTGCAAGA ACACTAAAGC AGATGCAGTG GTGAAGGATT TGTATGTTGA AAATGCCCAG 
TTGTTGAAAG CTCTGGAAAT GACTGAACAG CGACAAAAAA CAGCAGAGAA GAAAAATTAC 
CTCCTGGAAG AGAAGATTGC CAGCCTCAGT AATATAGTCA GGAATCTGGC ACCAGCACCA 
CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421992.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277700.1 Canis lupus familiaris ninein (NIN), transcript variant X4, mRNA.

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CloneID	OCe10361
Clone ID Related Accession (Same CDS sequence)	XM_537442.5 , XM_022421992.1
Accession Version	XM_022421992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGGAA ACCAGGAACA ACTAGTGACT GTCATGGAAG AACGAATGAT GGAAGTTGAA 
CAGAAGTTGA AACTGGTGAA GAGGCTTCTT CAGGAGAAAG TGAATCAGCT CAAAGAACAA 
CTCTGCAAGA ACACTAAAGC AGATGCAGTG GTGAAGGATT TGTATGTTGA AAATGCCCAG 
TTGTTGAAAG CTCTGGAAAT GACTGAACAG CGACAAAAAA CAGCAGAGAA GAAAAATTAC 
CTCCTGGAAG AGAAGATTGC CAGCCTCAGT AATATAGTCA GGAATCTGGC ACCAGCACCA 
CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277700.1
CDS	1..6390
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQGNQEQLVTVMEERMMEVEQKLKLVKRLLQEKVNQLKEQLCKNTKADAVVKDLYVENAQLLKALEMTEQRQKTAEKKNYLLEEKIASLSNIVRNLAPAPLTSIPPLRS

Target ORF information:

RefSeq Version	XM_022421992.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421992.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGGAA ACCAGGAACA ACTAGTGACT GTCATGGAAG AACGAATGAT GGAAGTTGAA 
CAGAAGTTGA AACTGGTGAA GAGGCTTCTT CAGGAGAAAG TGAATCAGCT CAAAGAACAA 
CTCTGCAAGA ACACTAAAGC AGATGCAGTG GTGAAGGATT TGTATGTTGA AAATGCCCAG 
TTGTTGAAAG CTCTGGAAAT GACTGAACAG CGACAAAAAA CAGCAGAGAA GAAAAATTAC 
CTCCTGGAAG AGAAGATTGC CAGCCTCAGT AATATAGTCA GGAATCTGGC ACCAGCACCA 
CTGACGTCTA TACCTCCCTT GAGGTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_022421996.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277704.1 Canis lupus familiaris ninein (NIN), transcript variant X10, mRNA.

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CloneID	OCe10364
Clone ID Related Accession (Same CDS sequence)	XM_022421996.1 , XM_003435130.3
Accession Version	XM_022421996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	ninein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGCCT TGCTACCGGA GCCGCGAGCA GTGCATGCAG ATTCCTACAG AAGGATTGGG 
CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277704.1
CDS	196..6324
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQALLPEPRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_022421996.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ninein (NIN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421996.1

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGCCT TGCTACCGGA GCCGCGAGCA GTGCATGCAG ATTCCTACAG AAGGATTGGG 
CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIN ( XM_003435130.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003435178.1 Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X9, mRNA.

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CloneID	OCe10364
Clone ID Related Accession (Same CDS sequence)	XM_022421996.1 , XM_003435130.3
Accession Version	XM_003435130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	ninein isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003435130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGCCT TGCTACCGGA GCCGCGAGCA GTGCATGCAG ATTCCTACAG AAGGATTGGG 
CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003435178.1
CDS	242..6370
Translation	MDEVEQDQHEARLKELFDSFDTTGTGSLGQEELTDLCHMLSLEEVAPVLQETLLQDNLLGRVHFDQFKEALILILSRTLSNEEHFQEPDCSLEAQPKYVKGGKRYGRRSLPEFQESVEEFAEIEPLDEEARPSHAPASDRGEHWKTQRSEEYEAEGQLRFWNPDDLNASQSGSSAPQDWIEEKLQEVCEDLGITRDGHLNRKKLVSICEQYGLQNIDGEILEEVFHNLDPDGTMSVEDFFYGLFKNGKSLTPSASTPYRQLKRHLSMQSFDESGRRTTTPSVMTSTIGFRVFSCLDDGMGYASVEKILDSWQEEGIENSQEVLKALDFSLDGNVNLTELTLALENELLGTKSGIHQAALASFKAEIRHLLERVDQVVREKEKLRSDLDKAEKLKSLMASEVDDHHAAIERRNEYNLRKLDEEYKERIAALKNELRKEREQILQQVGKQRLELEQEIEKAKTEENYIRDRLALSLKENSRLENELLENAEKLAEYENLTNKLQRNLENVLAEKFGDLDPSSAEFFLQEERLTQMRNEYEQQCRVLQDQVDELQSELEEFRTQGKVLRLPLKNSLSEELDVSRDCVEPDQGLASEECNPLNMSIEAELVIEQMKEQHHRDLCHLRQELEDKVHHYEKQLDETKVTCEKEQENMKQKYENEVHVLEKQISDLKNEIAELQGQAVVLKEAQRTTICRHEDEKKQLQRKWDEEKTHLQEKLKLEYELKLKASLEQAEESFNQEREGLIQNGAWTEEKVRGLTQELEQCHQEQLKSLMEKHSLEKEELRKELLEKHQRELQEGREKMETECTRRTSQIEAQFQADCQKVTERCENTLRSLESHYHQELKQLLEQQLEERSQWEFEKDELTQECMEAQEQLKETLQREKATSLVLAQEREMLEKTYKEHLNSMVVEREQLLKDLEDLRNVSRSQQSLLSDHILQLKGSHEREVEDSEQGLCQAGASEELASQQLEKLEMERDRERQEMLAKLQAMESVHKVTCEKADQERAEMSTEISWLQNKIKEMQQVVASLSRRESGCQAVAGEEAEGSGAMSLVQQGEQLLEENGDVLLSLQRAHERAVKENVKMATEISRLQQKLQKLEPGSVMSSCLDEPASGLFGNSAEQTEPFLLQNQMKQAEGGSTPCVLSDLQDDEARDLGSTGTSSAQRQEVKMEASEASIESFSELENSEETRTESWDLKNQICQLREQLMILRADCSRASEKKQDLLFDVSVLKKKLKMLERIPEASPKYKLLYEDASRENDCLQEELRVMEMRYDEALESNQELTSQVFKLQDELKKVEEVTETFLSLEKSYDEVRRENEELHVLVLRLQGKMEKLRERAVLQCDCFSLWEAHLGKLEVQPEGKVLGLQQTLDEHVPEVMNVHHLIEEQYLEQENTQLLERVQASEIAWLHRTIRTHGEKPKVQNQVILEEDTALLGLQDRHLPHGATIAELELEKKKLQELTRKLRERVAALVRQKDVPSQGENEEELKAMMHDLQITCSEMQQKVELLRYESEKLQEENSILRNEITTLNEEDSISNLKLGKLNGSQEEMWQKIETVKQEKAAVQKMAENLKKQISELKTKNQQLDLENIELSQKNSQNEKELQELNERLAEMLCQKDEEPGHSTFEEWKQEKSNLKEELEQCKAQSSALVSSLEAELSEVKIQTHMVEQENLLLKDELEKMKQLHRCPDLTDFQQKLSSVLSYNEKLLKEKEALSEELKSCVDKLAKSSLLEHRIATMKQEQKSWEHQSETLKSQLVASQEKVQNLEDTLQNVNLQMSLIKSDLRVTQQEKEALKQEVVSLYKQLQNADDKNWAPEIVSHPSGFHNQQQRLTWDKMDHLVKEEQQLLWQENEKLHTVVRNTKAELTHSREKVRQLESSLIPPKHQKHLNPSATMKPTEQEKLSLKRECEQFPKEQPPTNRKVSQMNSLERELETIHLENEGLKKKQVKLDEQLMEMQHLRSTAMLSPSPHAWDLQLRQQQACPTVPREQFLQLQQQLLQAERINQCLQEELENRTSETNTPQALLPEPRAVHADSYRRIGHL

Target ORF information:

RefSeq Version	XM_003435130.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ninein (GSK3B interacting protein) (NIN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003435130.3

ORF Insert Sequence:

ATGGATGAGG TGGAGCAGGA CCAGCATGAG GCTCGACTCA AGGAGCTGTT TGACAGTTTT 
GATACAACGG GCACCGGGTC CTTGGGACAG GAGGAACTCA CCGACCTTTG CCACATGTTG 
AGCTTGGAGG AGGTGGCCCC CGTGCTCCAG GAGACGCTGC TTCAGGACAA CCTGTTGGGC 
AGGGTGCATT TTGACCAATT TAAAGAAGCA CTAATACTTA TCTTGTCTAG AACTCTGTCA 
AATGAAGAGC ACTTTCAAGA ACCAGACTGC TCACTAGAAG CTCAGCCCAA ATATGTTAAA 
GGTGGGAAGC GTTATGGACG AAGGTCTTTA CCGGAGTTTC AAGAATCTGT GGAAGAGTTT 
GCAGAGATCG AGCCACTAGA TGAAGAAGCA CGGCCCTCAC ACGCCCCAGC CAGTGACCGC 
GGTGAGCACT GGAAGACACA ACGCAGTGAG GAGTATGAAG CAGAAGGCCA GTTAAGATTT 
TGGAACCCAG ATGACTTGAA TGCCTCACAG AGTGGGTCGT CCGCTCCCCA AGACTGGATA 
GAAGAGAAAC TGCAGGAGGT TTGTGAAGAT TTGGGAATCA CCCGTGATGG TCACCTGAAC 
CGAAAGAAGC TGGTCTCCAT CTGTGAACAG TATGGGCTGC AGAATATTGA TGGAGAGATA 
CTTGAGGAAG TCTTCCATAA TCTTGATCCT GATGGTACAA TGAGTGTGGA AGACTTTTTC 
TATGGCTTGT TCAAAAATGG AAAATCCCTC ACACCATCTG CATCTACGCC ATACAGACAG 
TTGAAAAGGC ACCTCTCCAT GCAGTCCTTC GATGAAAGTG GACGACGAAC CACAACCCCA 
TCTGTGATGA CAAGTACCAT TGGCTTTCGG GTCTTCTCCT GCCTGGACGA TGGGATGGGC 
TATGCATCGG TGGAGAAAAT CCTCGACTCC TGGCAAGAAG AGGGCATTGA GAACAGCCAG 
GAGGTCCTGA AGGCCTTGGA TTTCAGTCTC GATGGAAATG TGAACCTGAC AGAATTGACA 
CTGGCTCTTG AAAATGAACT TTTGGGCACC AAAAGTGGTA TTCACCAGGC AGCGCTGGCC 
AGCTTTAAGG CTGAGATCCG CCATTTACTG GAGCGAGTTG ATCAAGTCGT CAGAGAAAAA 
GAGAAGCTAC GGTCAGATCT GGACAAGGCT GAGAAGCTCA AGTCTCTAAT GGCTTCGGAG 
GTGGATGATC ACCATGCAGC CATAGAGCGG CGGAATGAGT ACAACCTCAG GAAACTGGAC 
GAAGAGTACA AGGAACGGAT AGCAGCCCTA AAGAATGAAC TCCGAAAGGA GAGAGAACAG 
ATCTTGCAGC AGGTGGGCAA GCAGCGTTTG GAACTTGAGC AGGAAATTGA AAAGGCAAAA 
ACAGAAGAGA ACTACATCCG GGACCGCCTT GCCCTCTCTT TAAAGGAAAA CAGTCGTCTA 
GAAAATGAGC TTCTGGAAAA TGCAGAGAAG CTGGCCGAAT ATGAAAATCT GACAAACAAA 
CTTCAGCGAA ATTTGGAAAA TGTGTTAGCA GAAAAGTTTG GTGACCTTGA TCCCAGCAGT 
GCTGAATTCT TTCTGCAAGA AGAGAGGCTG ACACAGATGA GAAATGAGTA TGAGCAGCAG 
TGTAGGGTAT TACAAGACCA AGTGGATGAA CTCCAGTCGG AGCTGGAAGA ATTTCGTACA 
CAAGGCAAAG TGTTGAGACT TCCCTTGAAG AACTCTCTGT CAGAAGAACT TGATGTTAGC 
AGGGATTGCG TTGAGCCTGA CCAGGGGCTC GCTTCTGAAG AATGCAATCC ATTGAATATG 
AGCATCGAGG CGGAGCTGGT CATTGAACAG ATGAAAGAAC AACATCACAG GGACTTATGT 
CACTTAAGAC AGGAGCTTGA AGATAAAGTG CACCATTATG AAAAGCAGCT AGATGAAACC 
AAGGTCACCT GTGAAAAGGA GCAAGAGAAC ATGAAGCAAA AGTATGAGAA TGAAGTGCAT 
GTCTTGGAAA AACAAATAAG TGACCTTAAA AATGAAATTG CAGAACTTCA GGGCCAGGCA 
GTGGTGCTCA AGGAGGCACA ACGTACAACT ATCTGCAGAC ACGAGGATGA GAAAAAGCAA 
CTGCAAAGGA AGTGGGATGA GGAAAAGACT CACCTGCAGG AGAAACTGAA GCTGGAATAT 
GAGTTGAAAC TCAAGGCTAG CCTGGAGCAG GCCGAGGAGA GCTTTAACCA AGAGAGAGAG 
GGACTCATTC AAAATGGCGC CTGGACAGAA GAGAAGGTGA GAGGCTTGAC TCAGGAACTA 
GAGCAGTGTC ACCAGGAGCA GCTGAAAAGC CTGATGGAGA AGCACAGTCT GGAAAAAGAG 
GAGTTGCGAA AAGAGCTCTT GGAAAAACAC CAAAGGGAAC TTCAAGAGGG AAGGGAAAAA 
ATGGAAACTG AGTGTACTAG AAGAACCTCT CAGATAGAAG CACAGTTTCA GGCTGATTGT 
CAGAAAGTCA CTGAGCGCTG TGAAAACACC CTGCGAAGCC TCGAGAGCCA CTACCACCAA 
GAGCTGAAGC AGCTCCTGGA GCAGCAGCTT GAGGAAAGAT CCCAGTGGGA GTTTGAGAAG 
GATGAGCTCA CCCAGGAGTG CATGGAAGCC CAGGAGCAGC TTAAGGAGAC TCTGCAGAGA 
GAGAAAGCAA CTTCTCTGGT CCTGGCCCAG GAGAGAGAGA TGCTGGAGAA AACATACAAG 
GAACATCTGA ATAGTATGGT TGTTGAAAGG GAGCAGCTAC TCAAAGACCT GGAAGATCTA 
AGAAACGTGT CCAGAAGTCA ACAAAGCCTA CTCTCCGACC ACATACTTCA GCTGAAGGGC 
AGTCATGAAA GGGAGGTGGA GGACTCCGAA CAGGGCCTGT GCCAGGCAGG GGCCTCCGAG 
GAGCTGGCCA GCCAGCAGCT TGAGAAGCTA GAAATGGAAC GTGACCGGGA GAGGCAGGAA 
ATGCTGGCCA AACTTCAGGC CATGGAGAGT GTCCACAAAG TGACCTGTGA GAAAGCGGAT 
CAGGAGAGGG CTGAGATGAG CACAGAAATC TCCTGGCTTC AGAACAAAAT TAAGGAAATG 
CAGCAGGTGG TGGCTTCTCT CTCCAGGAGA GAGAGTGGCT GCCAGGCAGT GGCAGGGGAG 
GAGGCAGAAG GAAGTGGAGC CATGTCCCTG GTTCAGCAAG GAGAGCAGTT GTTGGAAGAG 
AATGGAGATG TCCTCTTAAG CCTGCAGAGA GCTCATGAGC GAGCTGTGAA AGAAAACGTG 
AAAATGGCCA CGGAAATTTC AAGACTGCAA CAGAAACTGC AAAAATTAGA GCCAGGGTCG 
GTAATGTCGT CTTGTCTAGA TGAGCCAGCT TCCGGATTAT TTGGAAATTC TGCAGAACAA 
ACAGAGCCCT TTTTACTACA AAATCAAATG AAGCAAGCAG AAGGTGGAAG CACGCCATGC 
GTCCTAAGTG ACCTACAAGA CGACGAGGCA CGAGATCTGG GAAGTACAGG GACGAGCTCT 
GCTCAGAGAC AGGAGGTCAA AATGGAGGCA TCTGAAGCAT CAATAGAGAG TTTTTCCGAG 
CTCGAAAATA GTGAAGAGAC CAGGACTGAG AGCTGGGACC TGAAGAATCA GATTTGTCAG 
CTTCGGGAAC AGCTAATGAT CTTACGTGCA GATTGCAGTC GAGCTTCTGA AAAGAAACAG 
GACCTACTCT TTGATGTTTC TGTGCTGAAA AAGAAACTGA AGATGCTTGA GAGGATCCCT 
GAGGCCTCCC CCAAGTATAA ATTGTTATAT GAAGATGCCA GCAGGGAGAA TGACTGTCTC 
CAGGAAGAGC TGCGGGTGAT GGAGATGCGC TACGATGAAG CACTAGAAAG TAACCAGGAG 
CTCACTTCCC AAGTGTTCAA ACTGCAGGAT GAACTAAAGA AAGTGGAAGA GGTGACGGAA 
ACATTCCTCA GCCTGGAAAA GAGTTATGAT GAGGTCAGAA GAGAAAATGA GGAGCTGCAC 
GTTCTGGTTT TGAGACTTCA AGGCAAGATG GAGAAGCTTC GGGAAAGAGC AGTGCTACAG 
TGTGACTGCT TCTCCTTGTG GGAAGCCCAC TTAGGGAAGC TGGAAGTCCA GCCTGAGGGA 
AAGGTGCTTG GACTACAGCA GACACTGGAT GAGCATGTGC CAGAGGTGAT GAACGTACAT 
CATCTTATAG AAGAGCAGTA CCTCGAGCAG GAGAACACAC AGCTCCTGGA AAGAGTACAA 
GCAAGTGAAA TTGCCTGGCT ACACAGAACA ATCCGAACCC ATGGAGAAAA GCCCAAGGTA 
CAGAACCAGG TTATACTGGA GGAAGACACT GCTCTCCTAG GCCTTCAAGA CAGACATCTT 
CCACATGGGG CCACCATCGC AGAGTTAGAA CTGGAGAAAA AAAAGCTGCA AGAGCTGACT 
AGGAAGTTGA GGGAGAGGGT TGCTGCTTTA GTTAGGCAAA AAGATGTTCC TTCTCAAGGA 
GAAAACGAGG AAGAACTGAA GGCAATGATG CATGACTTGC AAATCACGTG CAGTGAGATG 
CAACAAAAAG TTGAACTTCT GAGATATGAA TCTGAGAAGC TTCAAGAAGA AAATTCTATT 
TTAAGAAATG AAATTACTAC TTTAAATGAA GAAGATAGCA TTTCTAACCT GAAATTAGGG 
AAATTAAATG GATCTCAGGA AGAAATGTGG CAAAAAATAG AAACTGTAAA ACAGGAAAAA 
GCGGCAGTTC AGAAGATGGC TGAAAATTTA AAGAAACAGA TTTCAGAATT AAAAACCAAA 
AACCAACAGT TGGATTTGGA AAATATAGAA CTTAGCCAAA AGAACTCTCA AAATGAGAAA 
GAACTGCAAG AACTTAATGA ACGTCTGGCA GAAATGCTAT GCCAGAAGGA TGAAGAGCCA 
GGACACAGTA CATTTGAGGA ATGGAAACAA GAAAAGTCTA ATCTGAAAGA AGAACTGGAA 
CAATGCAAAG CGCAGTCCTC AGCTTTAGTG TCTTCTCTGG AGGCAGAGCT CTCTGAAGTT 
AAAATACAGA CTCATATGGT GGAACAGGAA AACCTCCTTC TCAAAGATGA ACTGGAGAAA 
ATGAAACAAC TGCACAGATG TCCTGATCTC ACTGACTTCC AGCAAAAACT TTCTAGTGTT 
CTAAGCTACA ATGAAAAACT GCTGAAAGAA AAGGAAGCTC TGAGTGAAGA ATTAAAAAGC 
TGTGTGGATA AGTTGGCAAA ATCAAGTCTT TTAGAGCACA GAATAGCTAC TATGAAGCAA 
GAACAGAAGT CTTGGGAACA CCAGAGTGAA ACTTTAAAGT CCCAACTAGT GGCTTCACAG 
GAAAAGGTCC AGAATTTAGA AGACACCCTG CAGAATGTAA ACTTGCAAAT GTCGCTGATT 
AAATCTGACC TTCGAGTTAC TCAGCAGGAA AAGGAGGCTT TAAAACAAGA AGTGGTGTCT 
TTATATAAGC AACTTCAGAA TGCTGATGAC AAGAACTGGG CCCCAGAAAT AGTCAGCCAT 
CCATCAGGAT TCCATAACCA GCAGCAAAGG CTGACTTGGG ACAAGATGGA TCATCTGGTA 
AAGGAGGAAC AACAGCTGCT TTGGCAAGAG AATGAGAAAC TCCATACCGT GGTACGGAAC 
ACCAAAGCAG AGCTCACGCA CTCCCGGGAG AAGGTCCGTC AGTTGGAATC CAGCCTTATT 
CCTCCCAAGC ATCAGAAACA TCTAAACCCA TCAGCTACTA TGAAGCCCAC AGAGCAAGAG 
AAGTTGAGCT TAAAGAGAGA GTGTGAACAG TTTCCAAAGG AACAGCCTCC TACTAATAGG 
AAGGTCAGTC AGATGAATTC CCTTGAAAGA GAATTAGAAA CGATTCATTT GGAAAATGAA 
GGCCTGAAAA AGAAACAAGT AAAACTGGAT GAGCAGCTAA TGGAGATGCA GCACCTGAGG 
TCCACTGCGA TGCTTAGCCC GTCCCCTCAC GCTTGGGATC TCCAGCTGCG CCAGCAGCAA 
GCCTGTCCCA CGGTGCCCAG GGAGCAGTTT CTGCAGCTTC AACAACAGCT GCTGCAGGCA 
GAGAGGATAA ACCAGTGCCT GCAGGAGGAA CTTGAAAACA GGACCTCCGA AACCAACACC 
CCGCAGGCCT TGCTACCGGA GCCGCGAGCA GTGCATGCAG ATTCCTACAG AAGGATTGGG 
CACCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	NIN
Entrez Gene ID	480320
Full Name	ninein
General protein information	Preferred Names ninein Names ninein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	NIN	NP_065972.3
	NIN	XP_002805084.1
Gallus gallus (chicken)	NIN	XP_004936560.1
Rattus norvegicus (Norway rat)	Nin	NP_001100207.1
Rattus norvegicus (Norway rat)	LOC100911256	XP_006240209.1
Xenopus tropicalis (tropical clawed frog)	nin	XP_002936637.2
Pan troglodytes (chimpanzee)	NIN	XP_003314365.1
Canis lupus familiaris (dog)	NIN	XP_537442.2
Bos taurus (cattle)	NIN	XP_005211794.1
Mus musculus (house mouse)	Nin	NP_001074922.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

NIN cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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