HEATR5A gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022421883.1	XP_022277591.1	HEAT repeat-containing protein 5A isoform X1
XM_022421885.1	XP_022277593.1	HEAT repeat-containing protein 5A isoform X1
XM_022421887.1	XP_022277595.1	HEAT repeat-containing protein 5A isoform X1
XM_022421889.1	XP_022277597.1	HEAT repeat-containing protein 5A isoform X2
XM_856117.3	XP_861210.2	HEAT repeat-containing protein 5A isoform X4
XM_014115826.1	XP_013971301.1	HEAT repeat-containing protein 5A isoform X1
XM_014115827.1	XP_013971302.1	HEAT repeat-containing protein 5A isoform X1
XM_014115824.1	XP_013971299.1	HEAT repeat-containing protein 5A isoform X1
XM_014115828.1	XP_013971303.1	HEAT repeat-containing protein 5A isoform X1
XM_014115824.2	XP_013971299.1	HEAT repeat-containing protein 5A isoform X3
XM_014115831.2	XP_013971306.1	HEAT repeat-containing protein 5A isoform X4
XM_022421892.1	XP_022277600.1	HEAT repeat-containing protein 5A isoform X8
XM_014115829.1	XP_013971304.1	HEAT repeat-containing protein 5A isoform X1
XM_014115825.1	XP_013971300.1	HEAT repeat-containing protein 5A isoform X1
XM_022421890.1	XP_022277598.1	HEAT repeat-containing protein 5A isoform X5
XM_005623304.1	XP_005623361.1	HEAT repeat-containing protein 5A isoform X5
XM_856117.4	XP_861210.2	HEAT repeat-containing protein 5A isoform X6
XM_022421893.1	XP_022277601.1	HEAT repeat-containing protein 5A isoform X9
XM_014115830.1	XP_013971305.1	HEAT repeat-containing protein 5A isoform X2
XM_014115831.1	XP_013971306.1	HEAT repeat-containing protein 5A isoform X3
XM_022421884.1	XP_022277592.1	HEAT repeat-containing protein 5A isoform X1
XM_022421886.1	XP_022277594.1	HEAT repeat-containing protein 5A isoform X1
XM_022421888.1	XP_022277596.1	HEAT repeat-containing protein 5A isoform X1
XM_022421891.1	XP_022277599.1	HEAT repeat-containing protein 5A isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe290024	XM_022421893.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCe10254	XM_022421891.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290020	XM_022421886.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290020	XM_022421885.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290020	XM_022421888.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290020	XM_022421884.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290020	XM_022421883.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290020	XM_022421887.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290021	XM_022421889.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290022	XM_022421890.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe290023	XM_022421892.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37851	XM_014115824.2 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37851	XM_014115827.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37851	XM_014115828.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37851	XM_014115829.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37851	XM_014115826.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37851	XM_014115825.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37852	XM_014115830.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe37853	XM_014115831.2 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe10253	XM_856117.4 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe10256	XM_005623304.1 Latest version!	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe37851	XM_014115824.1	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe10253	XM_856117.3	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe37853	XM_014115831.1	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
Hide Old Accession Versions >>

HEATR5A ( XM_022421893.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277601.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OCe290024
Clone ID Related Accession (Same CDS sequence)	XM_022421893.1
Accession Version	XM_022421893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGTTGAT GATGGGGCTG 
CCGAAAAGGA AACCCTGCCA GAGTTTGGAG AGGGAAAGGA CACAGGAGGA CTTGTGCCTG 
GGAAATCTTT GGTTTTTGCA ACACTGGAAT TGTGTGTATG CATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277601.1
CDS	207..5174
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQLMMGLPKRKPCQSLERERTQEDLCLGNLWFLQHWNCVYAF

Target ORF information:

RefSeq Version	XM_022421893.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421893.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGTTGAT GATGGGGCTG 
CCGAAAAGGA AACCCTGCCA GAGTTTGGAG AGGGAAAGGA CACAGGAGGA CTTGTGCCTG 
GGAAATCTTT GGTTTTTGCA ACACTGGAAT TGTGTGTATG CATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421891.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277599.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X12, mRNA.

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CloneID	OCe10254
Clone ID Related Accession (Same CDS sequence)	XM_022421891.1
Accession Version	XM_022421891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGTCTCACGT 
CAAAGCATTC GCAGAGTATC TCTGGAGGAA GTTCTGGAAT TACTAGGAAC AGGGTTTCTA 
CGTGGAAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGC TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG AGTTACTCAG GCTTATGTGG TATTTGTTTC AACATTAGGA 
GGAACATGGC TAGAGAAAAA TTTTGCTACC TTTCTTTCTC ATATCCTAAG CCTTGTGTCA 
CAGTCGCACC CCAAAGCCAC CCAAACTCAG ACTGATGCTG TCTGCGGTCG CCGCTGTGTT 
TCATTTATCC TTCGAGCTAC TATAGGAGGT CTTCTTGGAG AAAAGGCCCA AATTGCTGCT 
GCCAAGGATA TTTGCCAGGC TATCTGGAAG TTAAAGAAAG TTGTGGATGC TGTGATGAAT 
GACGGTAATA TGGAAATCCG GCTTGGCTCC ACAGATGTGA CAGCCAGCCA GCATATGCTG 
GTGTGTGCTT TACAAGAACT TGGAAATCTC ATATACAGTC TTGGCACCAC AGCTGCACCT 
TTACTGCAGG ATTCAAGTGC AGGCATTCTT GATAGTGTCA TTTCAGTTAT TCTACATCCT 
AGCATTTCTG TTCGACTAGC AACGGCTTGG TGTTTACACT GCATTGCTGT GGCATTACCT 
TCTTACCTAA CACCTCTTTT AGACCGGTGC CTGGAACGGC TTACTGTACT TAAGTCTTCA 
CCGGAAGCAG TGACTGGCTT TAGTTTTGCT GTGGCAGCTT TGTTGGGAGC AGTGAAACAT 
TGTCCTTTAG GAATTCCTCA TGGGAAAGGC AAGATTATTA TGACTTTAGC AGAAGATTTA 
CTGTGTTCAG CTGTACAAAA CAGTCGTCTT TCAGCTCAGC GCACACAAGC TGGTTGGTTG 
TTGATTGCTG CTCTGATGAC ATTAGGTCCT GCAGTTGTCA GTCATCACCT TGCTCGACTT 
CTGCTATTGT GGAAGTGTGT CTTTCCAGTG TCTCCTAAAG ATCTAGAAAC AGAAAAGAGC 
CGAGGAGATT CATTTACCTG GCAGGTAACC TTGGAAGGAC GAGCTGGTGC GCTCTGTGCT 
GTCAAGAGTT TTGTTTCCCA CTGTGGTGAC CTTCTTACTG AGGAAGTAAT TCAACGTCTT 
CTTCCACCAC TTCCTTGTGC TGTGGATTTG CTAACTCAGC TTTCTTCAGT ACTAAAAACA 
TACGGAAGCA CTTTGAAAAT GCCATCAGTA GTTTATAGAC AAAGGCTTTA TGAATTACTG 
ATTTTATTAC CTCCTGAAAC CTATGAAGAA AACTTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACTTGA CTGCCTCTGA TATACAGGTG GCAGCATCTG CATTTTTACT TCCACCCCTC 
TGTCATCAGG ATGATCTTGT GATATTAAGT CCTTTGCTAC AAGAAACTGA TCATAGATTT 
ATTGAAGAAC AGCTCCTGCT TGGTAATGGT ATTGCTTGTG GAAGTTTGGA GTATGACCCT 
TATTCTATTT ATGAGAAAGA TGTAGAGGGA GATTCAGTGC CTAAACCCCT GTCTCCAGTA 
CTTTCAGTTA TTAGCTCTGC AGTCAAGCTC TTTGGAGTTA TATGTGCTCA TGTGGGAGAA 
GCTCAAAGGC TTTCTATACT GGAACAGCTT TTGGACAGTA TAAAGCACAC AAAAGGAGCT 
CGTCAACAAG TAGTTCAGTT ACATGTTGTT TCTTCACTCT CTAGTTTCTT GAAGTACTTA 
GCTGGCTCCA AGGGAAACTT GGGTTCAGAA GAAATGAGAA AACCTGCCCT AGCATTGGTG 
ATGGGAGCCC TAGAAAGTCC CAACCCCCTC TTGAGATGTG CAGCTGCAGA GGCATGGGCT 
AGATTAGCCC AAGTGGTTAA TGATGGAGCT TTTACTGCTG CATTAGCTCA AGTTAGCTTT 
GACAAACTGA AATCAGCAAG GGATGTGGTT ACCAGAACAG GATACTCATT GGCTTTGGGA 
TCTCTACATA GATATTTAGG AGGAATAAGT TCTTCTCAAC ATTTGAGTTC TTGTGTTGGA 
ATCCTTTATA CTTTGTCTCA GGACAGTACT TCTCCTGATG TGCAGACCTG GGCACTACAT 
TCGCTATCAT TGATAATTGA TTCTGCTGGC CCCCTCTATC ATATGCATGT GGAACCCACC 
CTTTCTCTTA TTATAATGTT ACTGTTAAAT GTGCCTCCTA CTCATGCTGA AGTACACCAG 
AGTCTTGGTC GCTGTTTGAA TGCCCTCATT ACTACATTGG GTCCAGAGCT ACAGGGTAAT 
AGTACTTCCA TTTCTACCTT AAGGACTTCC TGTCTTTTGG GTTGTGCAGT GATGCAAGAT 
AACCCAGACT GCCTCGTTCA AGCTCAGGCC ATCTCTTGCC TTCAGCAGCT TCATATGTTT 
GCACCAAGAC ATGTCAACTT GTCCAGCTTG GTCAGCTGCC TCTGTGTGAA TCTTTGTAGT 
CCCTATTTGT TACTGAGAAG AGCAGTACTG GCCTGTCTCC GTCAACTTGT ACAGAGAGAA 
GCAGCTGAAG TTTCAGAACA TGCTGTTTTG CTTGCTAAAG ACAACAGAGA AGAGTTGACT 
TCAGATGCTA ACATCAGAGA AGTGGGCCTT GAAGGGGCAT TATTGACCTT ACTAGATAAA 
GAAACAGACC AGAAGTTATG CCATGATATC AAAGAGACTC TAAATCATAT GCTTACATCT 
ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA AAGCTTTGTA AAGATGTACT TGCTGCATCA 
GCTGATTTTA CAACTGTGAC TTGTGTGGAT ACAATGCAAG AAGAGGAAGG AGATAAAGGG 
GATGATGCTT CAGTTCTGAC CACTGGAAAT GATGACAAGT CTTTTCCTTT CACCAATCCT 
CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA TGTGTCTGTA GGATAATTAA CCAGTGTGAG 
AATGCACACA GCGCACATTT TGACATTGCT TTAGCACAAG AAATGAAAAA AAGGGATTCA 
AGAAATGACT TTTTGGTGCT ACATCTTGCC GACTTAATTC GCATGGCCTT TATGGCTGCC 
ACAGATCACA GTGACCAGCT CCGACTTTCT GGCCTTGAAA CACTATTAGT TGTTATTCGG 
CGATTTGCAG CTATTCCAGA ACCAGAGTTT CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTAG GAGCTGCACT TAGGCCAGCC TTCACTTCAG AGACGCCACC TGATGTCACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGACCTTAAT 
GATTTACGAA GAGTTCATCA GTTGCTTGTT TCATCATTAA CAAAAATACA GGCTGGAAAA 
GAGGCTCTGA GTCACTTATA TAATGAAAGC GCTTCTACCA TGGAGATCTT AGCTGTGCTG 
AAAGCCTGGG CAGAGGTCTA CATAATTGCT GTAGAAAGAC ATAAAAGCCA CAGACAACCT 
TTGAAGACTA TCCCCTGTTC AGAAGAGAGT GTCAGAAATG GGCCACATTC AACAGATGGA 
CTGCTTGACT TAGTCCACAC AGACCTGAGC ACACTGAGTA GGCTCTGGCT TGCTGCACTT 
CAGGATTTTG CTCTCTTAAC TTTGCCTTCA GAATTTGCCT CCCAACTTCC TGTTGAAGGT 
GGTGCTTTCT ACACAGCAGA GACTAGTGAA AATGCAAAAT TACATTATTA CAACTCCTGG 
GCGCTTATCC TGCATGCTAC AGCATTGTGG CTTACCAGCA CAGGTTTTGT TGTTGCTGAT 
CCGGATGAAG AAGCAGCTCA TCTTTCAAGA CCTGTAACAC CAACTTCCAT GTGTCAGGGT 
TCATCATCTG GAGCTACAGT TAAGTCCCCT GAAGATGTCT GCACTGATAG ATTCCATCTT 
ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT TCCTTACGCT CAGATGCAAC CATGGAAAGC 
ATTACTGCTT GTTTACATGC CTTACAGGCC CTTCTAGATG TTCCTTGGCC CAGATCTAAA 
ATTGGCAGTG ATCAGGATTT GGGCATCGAG TTGTTGAATG TACTACATCG AGTAATTTTG 
ACCCGAGAAT CACCCTCTAT TCAATTGGCT TCACTTGAAG TGGTAAGACA GATTATCTGT 
GCAGCTCAAG AACATGTCAA GGAAAAAAGA CGAAGTGCAG AAGTTGATGA TGGGGCTGCC 
GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG GGAAAGGACA CAGGAGGACT TGTGCCTGGG 
AAATCTTTGG TTTTTGCAAC ACTGGAATTG TGTGTATGCA TTTTAGTTAG ACAGCTTCCA 
GAACTAAATC CTAAATTGAC AGGCAGCCCA GGAGTAAAAG CTACAAAGCC ACAGGTGCTG 
TCTGAGGATG GAAGTAGATT GGTGTCAGCT GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC 
GTGTGCTCTC CTGAAGGAAG CATCTCAATT CTCCCTACTA TATTATACCT TACAATTGGG 
GTCCTCAGAG AAACTGCTGT GAAGTTACCT GGAGGCCAGT TACCTTCAAC TGTTGCAGCT 
TCCCTACAGG CTCTGAAAGG AATATTGTCT TCTCCCATGG CCAGAGCAGA AAAGAGTCAC 
AGTGCTTGGA CTGACCTCCT CCGTAGTGCT TTAACAACAA TACTTGACTG TTGGGATCCA 
GTAGATGGAA CACATCAAGA ACTTGATGAA ATCAGTCTAC TTACTGCTAT CACAGTATTT 
ATTTTGTCTA CCAGTCCAGA AGTTACTACC ATCCCATGCC TTCAGAAGCG CTGTATTGAG 
AAATTTAAGG CTACTCTTGA GATAAAAGAT CCTGTGGTAC AAATCAAGAC CTATCAGCTA 
CTACTTTCCA TTTTTCAGTA TCCAAATCCA GCTGTTTCTT ACCCATACAT ATACTCTTTA 
GTATCCTCTA TTGTGGAAAA GCTACAGGAA ATAGACAAGA GAAAGCCTGA AGACACTGCT 
GAGCTTCAGA TCTTTCAAGA AGGCATAAAG GTCTTGCAAG CACTGGTGGC CATTGCCGAA 
GAACAACACC GTTCTCAGCT GGTGGCCTGT CTCCTGCCCA TCCTCATTTC CTTCCTTTTG 
GATGATAATG ATCTGGGATC TGCAACTTCT GTAATGAAAA ATTTACATGA CTTCGCTTTG 
CAAAATCTCA TGCAGATTGG GCCTCAGTAT TCATCAGTTT TTAAAAATGT AATGGCTTCT 
TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT GCTATCAAGG GCAATCAGGA AAGTGTAAAA 
ATCAAGATAC CAACATCTAA ACATACTAAG AACCCTGGAA AGAATACAAG CATCCAATTA 
AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277599.1
CDS	207..6344
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421891.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421891.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGTCTCACGT 
CAAAGCATTC GCAGAGTATC TCTGGAGGAA GTTCTGGAAT TACTAGGAAC AGGGTTTCTA 
CGTGGAAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGC TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG AGTTACTCAG GCTTATGTGG TATTTGTTTC AACATTAGGA 
GGAACATGGC TAGAGAAAAA TTTTGCTACC TTTCTTTCTC ATATCCTAAG CCTTGTGTCA 
CAGTCGCACC CCAAAGCCAC CCAAACTCAG ACTGATGCTG TCTGCGGTCG CCGCTGTGTT 
TCATTTATCC TTCGAGCTAC TATAGGAGGT CTTCTTGGAG AAAAGGCCCA AATTGCTGCT 
GCCAAGGATA TTTGCCAGGC TATCTGGAAG TTAAAGAAAG TTGTGGATGC TGTGATGAAT 
GACGGTAATA TGGAAATCCG GCTTGGCTCC ACAGATGTGA CAGCCAGCCA GCATATGCTG 
GTGTGTGCTT TACAAGAACT TGGAAATCTC ATATACAGTC TTGGCACCAC AGCTGCACCT 
TTACTGCAGG ATTCAAGTGC AGGCATTCTT GATAGTGTCA TTTCAGTTAT TCTACATCCT 
AGCATTTCTG TTCGACTAGC AACGGCTTGG TGTTTACACT GCATTGCTGT GGCATTACCT 
TCTTACCTAA CACCTCTTTT AGACCGGTGC CTGGAACGGC TTACTGTACT TAAGTCTTCA 
CCGGAAGCAG TGACTGGCTT TAGTTTTGCT GTGGCAGCTT TGTTGGGAGC AGTGAAACAT 
TGTCCTTTAG GAATTCCTCA TGGGAAAGGC AAGATTATTA TGACTTTAGC AGAAGATTTA 
CTGTGTTCAG CTGTACAAAA CAGTCGTCTT TCAGCTCAGC GCACACAAGC TGGTTGGTTG 
TTGATTGCTG CTCTGATGAC ATTAGGTCCT GCAGTTGTCA GTCATCACCT TGCTCGACTT 
CTGCTATTGT GGAAGTGTGT CTTTCCAGTG TCTCCTAAAG ATCTAGAAAC AGAAAAGAGC 
CGAGGAGATT CATTTACCTG GCAGGTAACC TTGGAAGGAC GAGCTGGTGC GCTCTGTGCT 
GTCAAGAGTT TTGTTTCCCA CTGTGGTGAC CTTCTTACTG AGGAAGTAAT TCAACGTCTT 
CTTCCACCAC TTCCTTGTGC TGTGGATTTG CTAACTCAGC TTTCTTCAGT ACTAAAAACA 
TACGGAAGCA CTTTGAAAAT GCCATCAGTA GTTTATAGAC AAAGGCTTTA TGAATTACTG 
ATTTTATTAC CTCCTGAAAC CTATGAAGAA AACTTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACTTGA CTGCCTCTGA TATACAGGTG GCAGCATCTG CATTTTTACT TCCACCCCTC 
TGTCATCAGG ATGATCTTGT GATATTAAGT CCTTTGCTAC AAGAAACTGA TCATAGATTT 
ATTGAAGAAC AGCTCCTGCT TGGTAATGGT ATTGCTTGTG GAAGTTTGGA GTATGACCCT 
TATTCTATTT ATGAGAAAGA TGTAGAGGGA GATTCAGTGC CTAAACCCCT GTCTCCAGTA 
CTTTCAGTTA TTAGCTCTGC AGTCAAGCTC TTTGGAGTTA TATGTGCTCA TGTGGGAGAA 
GCTCAAAGGC TTTCTATACT GGAACAGCTT TTGGACAGTA TAAAGCACAC AAAAGGAGCT 
CGTCAACAAG TAGTTCAGTT ACATGTTGTT TCTTCACTCT CTAGTTTCTT GAAGTACTTA 
GCTGGCTCCA AGGGAAACTT GGGTTCAGAA GAAATGAGAA AACCTGCCCT AGCATTGGTG 
ATGGGAGCCC TAGAAAGTCC CAACCCCCTC TTGAGATGTG CAGCTGCAGA GGCATGGGCT 
AGATTAGCCC AAGTGGTTAA TGATGGAGCT TTTACTGCTG CATTAGCTCA AGTTAGCTTT 
GACAAACTGA AATCAGCAAG GGATGTGGTT ACCAGAACAG GATACTCATT GGCTTTGGGA 
TCTCTACATA GATATTTAGG AGGAATAAGT TCTTCTCAAC ATTTGAGTTC TTGTGTTGGA 
ATCCTTTATA CTTTGTCTCA GGACAGTACT TCTCCTGATG TGCAGACCTG GGCACTACAT 
TCGCTATCAT TGATAATTGA TTCTGCTGGC CCCCTCTATC ATATGCATGT GGAACCCACC 
CTTTCTCTTA TTATAATGTT ACTGTTAAAT GTGCCTCCTA CTCATGCTGA AGTACACCAG 
AGTCTTGGTC GCTGTTTGAA TGCCCTCATT ACTACATTGG GTCCAGAGCT ACAGGGTAAT 
AGTACTTCCA TTTCTACCTT AAGGACTTCC TGTCTTTTGG GTTGTGCAGT GATGCAAGAT 
AACCCAGACT GCCTCGTTCA AGCTCAGGCC ATCTCTTGCC TTCAGCAGCT TCATATGTTT 
GCACCAAGAC ATGTCAACTT GTCCAGCTTG GTCAGCTGCC TCTGTGTGAA TCTTTGTAGT 
CCCTATTTGT TACTGAGAAG AGCAGTACTG GCCTGTCTCC GTCAACTTGT ACAGAGAGAA 
GCAGCTGAAG TTTCAGAACA TGCTGTTTTG CTTGCTAAAG ACAACAGAGA AGAGTTGACT 
TCAGATGCTA ACATCAGAGA AGTGGGCCTT GAAGGGGCAT TATTGACCTT ACTAGATAAA 
GAAACAGACC AGAAGTTATG CCATGATATC AAAGAGACTC TAAATCATAT GCTTACATCT 
ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA AAGCTTTGTA AAGATGTACT TGCTGCATCA 
GCTGATTTTA CAACTGTGAC TTGTGTGGAT ACAATGCAAG AAGAGGAAGG AGATAAAGGG 
GATGATGCTT CAGTTCTGAC CACTGGAAAT GATGACAAGT CTTTTCCTTT CACCAATCCT 
CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA TGTGTCTGTA GGATAATTAA CCAGTGTGAG 
AATGCACACA GCGCACATTT TGACATTGCT TTAGCACAAG AAATGAAAAA AAGGGATTCA 
AGAAATGACT TTTTGGTGCT ACATCTTGCC GACTTAATTC GCATGGCCTT TATGGCTGCC 
ACAGATCACA GTGACCAGCT CCGACTTTCT GGCCTTGAAA CACTATTAGT TGTTATTCGG 
CGATTTGCAG CTATTCCAGA ACCAGAGTTT CCAGGTCATG TGATTCTGGA ACAGTATCAA 
GCCAATGTAG GAGCTGCACT TAGGCCAGCC TTCACTTCAG AGACGCCACC TGATGTCACT 
GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG ATAGCAAGTG GAGTTGTAAG TGACCTTAAT 
GATTTACGAA GAGTTCATCA GTTGCTTGTT TCATCATTAA CAAAAATACA GGCTGGAAAA 
GAGGCTCTGA GTCACTTATA TAATGAAAGC GCTTCTACCA TGGAGATCTT AGCTGTGCTG 
AAAGCCTGGG CAGAGGTCTA CATAATTGCT GTAGAAAGAC ATAAAAGCCA CAGACAACCT 
TTGAAGACTA TCCCCTGTTC AGAAGAGAGT GTCAGAAATG GGCCACATTC AACAGATGGA 
CTGCTTGACT TAGTCCACAC AGACCTGAGC ACACTGAGTA GGCTCTGGCT TGCTGCACTT 
CAGGATTTTG CTCTCTTAAC TTTGCCTTCA GAATTTGCCT CCCAACTTCC TGTTGAAGGT 
GGTGCTTTCT ACACAGCAGA GACTAGTGAA AATGCAAAAT TACATTATTA CAACTCCTGG 
GCGCTTATCC TGCATGCTAC AGCATTGTGG CTTACCAGCA CAGGTTTTGT TGTTGCTGAT 
CCGGATGAAG AAGCAGCTCA TCTTTCAAGA CCTGTAACAC CAACTTCCAT GTGTCAGGGT 
TCATCATCTG GAGCTACAGT TAAGTCCCCT GAAGATGTCT GCACTGATAG ATTCCATCTT 
ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT TCCTTACGCT CAGATGCAAC CATGGAAAGC 
ATTACTGCTT GTTTACATGC CTTACAGGCC CTTCTAGATG TTCCTTGGCC CAGATCTAAA 
ATTGGCAGTG ATCAGGATTT GGGCATCGAG TTGTTGAATG TACTACATCG AGTAATTTTG 
ACCCGAGAAT CACCCTCTAT TCAATTGGCT TCACTTGAAG TGGTAAGACA GATTATCTGT 
GCAGCTCAAG AACATGTCAA GGAAAAAAGA CGAAGTGCAG AAGTTGATGA TGGGGCTGCC 
GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG GGAAAGGACA CAGGAGGACT TGTGCCTGGG 
AAATCTTTGG TTTTTGCAAC ACTGGAATTG TGTGTATGCA TTTTAGTTAG ACAGCTTCCA 
GAACTAAATC CTAAATTGAC AGGCAGCCCA GGAGTAAAAG CTACAAAGCC ACAGGTGCTG 
TCTGAGGATG GAAGTAGATT GGTGTCAGCT GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC 
GTGTGCTCTC CTGAAGGAAG CATCTCAATT CTCCCTACTA TATTATACCT TACAATTGGG 
GTCCTCAGAG AAACTGCTGT GAAGTTACCT GGAGGCCAGT TACCTTCAAC TGTTGCAGCT 
TCCCTACAGG CTCTGAAAGG AATATTGTCT TCTCCCATGG CCAGAGCAGA AAAGAGTCAC 
AGTGCTTGGA CTGACCTCCT CCGTAGTGCT TTAACAACAA TACTTGACTG TTGGGATCCA 
GTAGATGGAA CACATCAAGA ACTTGATGAA ATCAGTCTAC TTACTGCTAT CACAGTATTT 
ATTTTGTCTA CCAGTCCAGA AGTTACTACC ATCCCATGCC TTCAGAAGCG CTGTATTGAG 
AAATTTAAGG CTACTCTTGA GATAAAAGAT CCTGTGGTAC AAATCAAGAC CTATCAGCTA 
CTACTTTCCA TTTTTCAGTA TCCAAATCCA GCTGTTTCTT ACCCATACAT ATACTCTTTA 
GTATCCTCTA TTGTGGAAAA GCTACAGGAA ATAGACAAGA GAAAGCCTGA AGACACTGCT 
GAGCTTCAGA TCTTTCAAGA AGGCATAAAG GTCTTGCAAG CACTGGTGGC CATTGCCGAA 
GAACAACACC GTTCTCAGCT GGTGGCCTGT CTCCTGCCCA TCCTCATTTC CTTCCTTTTG 
GATGATAATG ATCTGGGATC TGCAACTTCT GTAATGAAAA ATTTACATGA CTTCGCTTTG 
CAAAATCTCA TGCAGATTGG GCCTCAGTAT TCATCAGTTT TTAAAAATGT AATGGCTTCT 
TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT GCTATCAAGG GCAATCAGGA AAGTGTAAAA 
ATCAAGATAC CAACATCTAA ACATACTAAG AACCCTGGAA AGAATACAAG CATCCAATTA 
AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421883.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277591.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

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CloneID	OCe290020
Clone ID Related Accession (Same CDS sequence)	XM_022421883.1 , XM_022421885.1 , XM_022421887.1 , XM_022421884.1 , XM_022421886.1 , XM_022421888.1
Accession Version	XM_022421883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277591.1
CDS	207..6389
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421883.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421883.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421885.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277593.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.

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CloneID	OCe290020
Clone ID Related Accession (Same CDS sequence)	XM_022421883.1 , XM_022421885.1 , XM_022421887.1 , XM_022421884.1 , XM_022421886.1 , XM_022421888.1
Accession Version	XM_022421885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277593.1
CDS	156..6338
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421885.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421885.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421887.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277595.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.

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CloneID	OCe290020
Clone ID Related Accession (Same CDS sequence)	XM_022421883.1 , XM_022421885.1 , XM_022421887.1 , XM_022421884.1 , XM_022421886.1 , XM_022421888.1
Accession Version	XM_022421887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277595.1
CDS	173..6355
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421887.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421887.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421884.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277592.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

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CloneID	OCe290020
Clone ID Related Accession (Same CDS sequence)	XM_022421883.1 , XM_022421885.1 , XM_022421887.1 , XM_022421884.1 , XM_022421886.1 , XM_022421888.1
Accession Version	XM_022421884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277592.1
CDS	267..6449
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421884.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421884.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421886.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277594.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.

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CloneID	OCe290020
Clone ID Related Accession (Same CDS sequence)	XM_022421883.1 , XM_022421885.1 , XM_022421887.1 , XM_022421884.1 , XM_022421886.1 , XM_022421888.1
Accession Version	XM_022421886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277594.1
CDS	257..6439
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421886.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421886.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421888.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277596.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X6, mRNA.

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CloneID	OCe290020
Clone ID Related Accession (Same CDS sequence)	XM_022421883.1 , XM_022421885.1 , XM_022421887.1 , XM_022421884.1 , XM_022421886.1 , XM_022421888.1
Accession Version	XM_022421888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277596.1
CDS	205..6387
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421888.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421888.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATCATTTCT CTTTAGTAGA TGGAACACAT 
CAAGAACTTG ATGAAATCAG TCTACTTACT GCTATCACAG TATTTATTTT GTCTACCAGT 
CCAGAAGTTA CTACCATCCC ATGCCTTCAG AAGCGCTGTA TTGAGAAATT TAAGGCTACT 
CTTGAGATAA AAGATCCTGT GGTACAAATC AAGACCTATC AGCTACTACT TTCCATTTTT 
CAGTATCCAA ATCCAGCTGT TTCTTACCCA TACATATACT CTTTAGTATC CTCTATTGTG 
GAAAAGCTAC AGGAAATAGA CAAGAGAAAG CCTGAAGACA CTGCTGAGCT TCAGATCTTT 
CAAGAAGGCA TAAAGGTCTT GCAAGCACTG GTGGCCATTG CCGAAGAACA ACACCGTTCT 
CAGCTGGTGG CCTGTCTCCT GCCCATCCTC ATTTCCTTCC TTTTGGATGA TAATGATCTG 
GGATCTGCAA CTTCTGTAAT GAAAAATTTA CATGACTTCG CTTTGCAAAA TCTCATGCAG 
ATTGGGCCTC AGTATTCATC AGTTTTTAAA AATGTAATGG CTTCTTCTCC AGCCCTGAAA 
GCCCGCCTTG AGGCTGCTAT CAAGGGCAAT CAGGAAAGTG TAAAAATCAA GATACCAACA 
TCTAAACATA CTAAGAACCC TGGAAAGAAT ACAAGCATCC AATTAAAGAC CAACTTCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421889.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277597.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X7, mRNA.

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CloneID	OCe290021
Clone ID Related Accession (Same CDS sequence)	XM_022421889.1
Accession Version	XM_022421889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6180bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGTCTCACGT 
CAAAGCATTC GCAGAGTATC TCTGGAGGAA GTTCTGGAAT TACTAGGAAC AGGGTTTCTA 
CGTGGAAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGC TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG AGTTACTCAG GCTTATGTGG TATTTGTTTC AACATTAGGA 
GGAACATGGC TAGAGAAAAA TTTTGCTACC TTTCTTTCTC ATATCCTAAG CCTTGTGTCA 
CAGTCGCACC CCAAAGCCAC CCAAACTCAG ACTGATGCTG TCTGCGGTCG CCGCTGTGTT 
TCATTTATCC TTCGAGCTAC TATAGGAGGT CTTCTTGGAG AAAAGGCCCA AATTGCTGCT 
GCCAAGGATA TTTGCCAGGC TATCTGGAAG TTAAAGAAAG TTGTGGATGC TGTGATGAAT 
GACGGTAATA TGGAAATCCG GCTTGGCTCC ACAGATGTGA CAGCCAGCCA GCATATGCTG 
GTGTGTGCTT TACAAGAACT TGGAAATCTC ATATACAGTC TTGGCACCAC AGCTGCACCT 
TTACTGCAGG ATTCAAGTGC AGGCATTCTT GATAGTGTCA TTTCAGTTAT TCTACATCCT 
AGCATTTCTG TTCGACTAGC AACGGCTTGG TGTTTACACT GCATTGCTGT GGCATTACCT 
TCTTACCTAA CACCTCTTTT AGACCGGTGC CTGGAACGGC TTACTGTACT TAAGTCTTCA 
CCGGAAGCAG TGACTGGCTT TAGTTTTGCT GTGGCAGCTT TGTTGGGAGC AGTGAAACAT 
TGTCCTTTAG GAATTCCTCA TGGGAAAGGC AAGATTATTA TGACTTTAGC AGAAGATTTA 
CTGTGTTCAG CTGTACAAAA CAGTCGTCTT TCAGCTCAGC GCACACAAGC TGGTTGGTTG 
TTGATTGCTG CTCTGATGAC ATTAGGTCCT GCAGTTGTCA GTCATCACCT TGCTCGACTT 
CTGCTATTGT GGAAGTGTGT CTTTCCAGTG TCTCCTAAAG ATCTAGAAAC AGAAAAGAGC 
CGAGGAGATT CATTTACCTG GCAGGTAACC TTGGAAGGAC GAGCTGGTGC GCTCTGTGCT 
GTCAAGAGTT TTGTTTCCCA CTGTGGTGAC CTTCTTACTG AGGAAGTAAT TCAACGTCTT 
CTTCCACCAC TTCCTTGTGC TGTGGATTTG CTAACTCAGC TTTCTTCAGT ACTAAAAACA 
TACGGAAGCA CTTTGAAAAT GCCATCAGTA GTTTATAGAC AAAGGCTTTA TGAATTACTG 
ATTTTATTAC CTCCTGAAAC CTATGAAGAA AACTTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACTTGA CTGCCTCTGA TATACAGGTG GCAGCATCTG CATTTTTACT TCCACCCCTC 
TGTCATCAGG ATGATCTTGT GATATTAAGT CCTTTGCTAC AAGAAACTGA TCATAGATTT 
ATTGAAGAAC AGCTCCTGCT TGGTAATGGT ATTGCTTGTG GAAGTTTGGA GTATGACCCT 
TATTCTATTT ATGAGAAAGA TGTAGAGGGA GATTCAGTGC CTAAACCCCT GTCTCCAGTA 
CTTTCAGTTA TTAGCTCTGC AGTCAAGCTC TTTGGAGTTA TATGTGCTCA TGTGGGAGAA 
GCTCAAAGGC TTTCTATACT GGAACAGCTT TTGGACAGTA TAAAGCACAC AAAAGGAGCT 
CGTCAACAAG TAGTTCAGTT ACATGTTGTT TCTTCACTCT CTAGTTTCTT GAAGTACTTA 
GCTGGCTCCA AGGGAAACTT GGGTTCAGAA GAAATGAGAA AACCTGCCCT AGCATTGGTG 
ATGGGAGCCC TAGAAAGTCC CAACCCCCTC TTGAGATGTG CAGCTGCAGA GGCATGGGCT 
AGATTAGCCC AAGTGGTTAA TGATGGAGCT TTTACTGCTG CATTAGCTCA AGTTAGCTTT 
GACAAACTGA AATCAGCAAG GGATGTGGTT ACCAGAACAG GATACTCATT GGCTTTGGGA 
TCTCTACATA GATATTTAGG AGGAATAAGT TCTTCTCAAC ATTTGAGTTC TTGTGTTGGA 
ATCCTTTATA CTTTGTCTCA GGACAGTACT TCTCCTGATG TGCAGACCTG GGCACTACAT 
TCGCTATCAT TGATAATTGA TTCTGCTGGC CCCCTCTATC ATATGCATGT GGAACCCACC 
CTTTCTCTTA TTATAATGTT ACTGTTAAAT GTGCCTCCTA CTCATGCTGA AGTACACCAG 
AGTCTTGGTC GCTGTTTGAA TGCCCTCATT ACTACATTGG GTCCAGAGCT ACAGGGTAAT 
AGTACTTCCA TTTCTACCTT AAGGACTTCC TGTCTTTTGG GTTGTGCAGT GATGCAAGAT 
AACCCAGACT GCCTCGTTCA AGCTCAGGCC ATCTCTTGCC TTCAGCAGCT TCATATGTTT 
GCACCAAGAC ATGTCAACTT GTCCAGCTTG GTCAGCTGCC TCTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT TTGTAGTCCC TATTTGTTAC TGAGAAGAGC AGTACTGGCC 
TGTCTCCGTC AACTTGTACA GAGAGAAGCA GCTGAAGTTT CAGAACATGC TGTTTTGCTT 
GCTAAAGACA ACAGAGAAGA GTTGACTTCA GATGCTAACA TCAGAGAAGT GGGCCTTGAA 
GGGGCATTAT TGACCTTACT AGATAAAGAA ACAGACCAGA AGTTATGCCA TGATATCAAA 
GAGACTCTAA ATCATATGCT TACATCTATG GCAGTGGATA AGCTCTCCTT CTGGTTAAAG 
CTTTGTAAAG ATGTACTTGC TGCATCAGCT GATTTTACAA CTGTGACTTG TGTGGATACA 
ATGCAAGAAG AGGAAGGAGA TAAAGGGGAT GATGCTTCAG TTCTGACCAC TGGAAATGAT 
GACAAGTCTT TTCCTTTCAC CAATCCTCGT TGGGCTACTA GAGTCTTTGC TGCCGAATGT 
GTCTGTAGGA TAATTAACCA GTGTGAGAAT GCACACAGCG CACATTTTGA CATTGCTTTA 
GCACAAGAAA TGAAAAAAAG GGATTCAAGA AATGACTTTT TGGTGCTACA TCTTGCCGAC 
TTAATTCGCA TGGCCTTTAT GGCTGCCACA GATCACAGTG ACCAGCTCCG ACTTTCTGGC 
CTTGAAACAC TATTAGTTGT TATTCGGCGA TTTGCAGCTA TTCCAGAACC AGAGTTTCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTAGGAG CTGCACTTAG GCCAGCCTTC 
ACTTCAGAGA CGCCACCTGA TGTCACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA CCTTAATGAT TTACGAAGAG TTCATCAGTT GCTTGTTTCA 
TCATTAACAA AAATACAGGC TGGAAAAGAG GCTCTGAGTC ACTTATATAA TGAAAGCGCT 
TCTACCATGG AGATCTTAGC TGTGCTGAAA GCCTGGGCAG AGGTCTACAT AATTGCTGTA 
GAAAGACATA AAAGCCACAG ACAACCTTTG AAGACTATCC CCTGTTCAGA AGAGAGTGTC 
AGAAATGGGC CACATTCAAC AGATGGACTG CTTGACTTAG TCCACACAGA CCTGAGCACA 
CTGAGTAGGC TCTGGCTTGC TGCACTTCAG GATTTTGCTC TCTTAACTTT GCCTTCAGAA 
TTTGCCTCCC AACTTCCTGT TGAAGGTGGT GCTTTCTACA CAGCAGAGAC TAGTGAAAAT 
GCAAAATTAC ATTATTACAA CTCCTGGGCG CTTATCCTGC ATGCTACAGC ATTGTGGCTT 
ACCAGCACAG GTTTTGTTGT TGCTGATCCG GATGAAGAAG CAGCTCATCT TTCAAGACCT 
GTAACACCAA CTTCCATGTG TCAGGGTTCA TCATCTGGAG CTACAGTTAA GTCCCCTGAA 
GATGTCTGCA CTGATAGATT CCATCTTATT TTAGGAATAA GTGTGGAGTT TCTGTGTTCC 
TTACGCTCAG ATGCAACCAT GGAAAGCATT ACTGCTTGTT TACATGCCTT ACAGGCCCTT 
CTAGATGTTC CTTGGCCCAG ATCTAAAATT GGCAGTGATC AGGATTTGGG CATCGAGTTG 
TTGAATGTAC TACATCGAGT AATTTTGACC CGAGAATCAC CCTCTATTCA ATTGGCTTCA 
CTTGAAGTGG TAAGACAGAT TATCTGTGCA GCTCAAGAAC ATGTCAAGGA AAAAAGACGA 
AGTGCAGAAG TTGATGATGG GGCTGCCGAA AAGGAAACCC TGCCAGAGTT TGGAGAGGGA 
AAGGACACAG GAGGACTTGT GCCTGGGAAA TCTTTGGTTT TTGCAACACT GGAATTGTGT 
GTATGCATTT TAGTTAGACA GCTTCCAGAA CTAAATCCTA AATTGACAGG CAGCCCAGGA 
GTAAAAGCTA CAAAGCCACA GGTGCTGTCT GAGGATGGAA GTAGATTGGT GTCAGCTGCG 
TTAGTTATCC TCTCTGAACT TCCTGCCGTG TGCTCTCCTG AAGGAAGCAT CTCAATTCTC 
CCTACTATAT TATACCTTAC AATTGGGGTC CTCAGAGAAA CTGCTGTGAA GTTACCTGGA 
GGCCAGTTAC CTTCAACTGT TGCAGCTTCC CTACAGGCTC TGAAAGGAAT ATTGTCTTCT 
CCCATGGCCA GAGCAGAAAA GAGTCACAGT GCTTGGACTG ACCTCCTCCG TAGTGCTTTA 
ACAACAATAC TTGACTGTTG GGATCCAGAT CATTTCTCTT TAGTAGATGG AACACATCAA 
GAACTTGATG AAATCAGTCT ACTTACTGCT ATCACAGTAT TTATTTTGTC TACCAGTCCA 
GAAGTTACTA CCATCCCATG CCTTCAGAAG CGCTGTATTG AGAAATTTAA GGCTACTCTT 
GAGATAAAAG ATCCTGTGGT ACAAATCAAG ACCTATCAGC TACTACTTTC CATTTTTCAG 
TATCCAAATC CAGCTGTTTC TTACCCATAC ATATACTCTT TAGTATCCTC TATTGTGGAA 
AAGCTACAGG AAATAGACAA GAGAAAGCCT GAAGACACTG CTGAGCTTCA GATCTTTCAA 
GAAGGCATAA AGGTCTTGCA AGCACTGGTG GCCATTGCCG AAGAACAACA CCGTTCTCAG 
CTGGTGGCCT GTCTCCTGCC CATCCTCATT TCCTTCCTTT TGGATGATAA TGATCTGGGA 
TCTGCAACTT CTGTAATGAA AAATTTACAT GACTTCGCTT TGCAAAATCT CATGCAGATT 
GGGCCTCAGT ATTCATCAGT TTTTAAAAAT GTAATGGCTT CTTCTCCAGC CCTGAAAGCC 
CGCCTTGAGG CTGCTATCAA GGGCAATCAG GAAAGTGTAA AAATCAAGAT ACCAACATCT 
AAACATACTA AGAACCCTGG AAAGAATACA AGCATCCAAT TAAAGACCAA CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277597.1
CDS	207..6386
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421889.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421889.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGTCTCACGT 
CAAAGCATTC GCAGAGTATC TCTGGAGGAA GTTCTGGAAT TACTAGGAAC AGGGTTTCTA 
CGTGGAAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGC TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG AGTTACTCAG GCTTATGTGG TATTTGTTTC AACATTAGGA 
GGAACATGGC TAGAGAAAAA TTTTGCTACC TTTCTTTCTC ATATCCTAAG CCTTGTGTCA 
CAGTCGCACC CCAAAGCCAC CCAAACTCAG ACTGATGCTG TCTGCGGTCG CCGCTGTGTT 
TCATTTATCC TTCGAGCTAC TATAGGAGGT CTTCTTGGAG AAAAGGCCCA AATTGCTGCT 
GCCAAGGATA TTTGCCAGGC TATCTGGAAG TTAAAGAAAG TTGTGGATGC TGTGATGAAT 
GACGGTAATA TGGAAATCCG GCTTGGCTCC ACAGATGTGA CAGCCAGCCA GCATATGCTG 
GTGTGTGCTT TACAAGAACT TGGAAATCTC ATATACAGTC TTGGCACCAC AGCTGCACCT 
TTACTGCAGG ATTCAAGTGC AGGCATTCTT GATAGTGTCA TTTCAGTTAT TCTACATCCT 
AGCATTTCTG TTCGACTAGC AACGGCTTGG TGTTTACACT GCATTGCTGT GGCATTACCT 
TCTTACCTAA CACCTCTTTT AGACCGGTGC CTGGAACGGC TTACTGTACT TAAGTCTTCA 
CCGGAAGCAG TGACTGGCTT TAGTTTTGCT GTGGCAGCTT TGTTGGGAGC AGTGAAACAT 
TGTCCTTTAG GAATTCCTCA TGGGAAAGGC AAGATTATTA TGACTTTAGC AGAAGATTTA 
CTGTGTTCAG CTGTACAAAA CAGTCGTCTT TCAGCTCAGC GCACACAAGC TGGTTGGTTG 
TTGATTGCTG CTCTGATGAC ATTAGGTCCT GCAGTTGTCA GTCATCACCT TGCTCGACTT 
CTGCTATTGT GGAAGTGTGT CTTTCCAGTG TCTCCTAAAG ATCTAGAAAC AGAAAAGAGC 
CGAGGAGATT CATTTACCTG GCAGGTAACC TTGGAAGGAC GAGCTGGTGC GCTCTGTGCT 
GTCAAGAGTT TTGTTTCCCA CTGTGGTGAC CTTCTTACTG AGGAAGTAAT TCAACGTCTT 
CTTCCACCAC TTCCTTGTGC TGTGGATTTG CTAACTCAGC TTTCTTCAGT ACTAAAAACA 
TACGGAAGCA CTTTGAAAAT GCCATCAGTA GTTTATAGAC AAAGGCTTTA TGAATTACTG 
ATTTTATTAC CTCCTGAAAC CTATGAAGAA AACTTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACTTGA CTGCCTCTGA TATACAGGTG GCAGCATCTG CATTTTTACT TCCACCCCTC 
TGTCATCAGG ATGATCTTGT GATATTAAGT CCTTTGCTAC AAGAAACTGA TCATAGATTT 
ATTGAAGAAC AGCTCCTGCT TGGTAATGGT ATTGCTTGTG GAAGTTTGGA GTATGACCCT 
TATTCTATTT ATGAGAAAGA TGTAGAGGGA GATTCAGTGC CTAAACCCCT GTCTCCAGTA 
CTTTCAGTTA TTAGCTCTGC AGTCAAGCTC TTTGGAGTTA TATGTGCTCA TGTGGGAGAA 
GCTCAAAGGC TTTCTATACT GGAACAGCTT TTGGACAGTA TAAAGCACAC AAAAGGAGCT 
CGTCAACAAG TAGTTCAGTT ACATGTTGTT TCTTCACTCT CTAGTTTCTT GAAGTACTTA 
GCTGGCTCCA AGGGAAACTT GGGTTCAGAA GAAATGAGAA AACCTGCCCT AGCATTGGTG 
ATGGGAGCCC TAGAAAGTCC CAACCCCCTC TTGAGATGTG CAGCTGCAGA GGCATGGGCT 
AGATTAGCCC AAGTGGTTAA TGATGGAGCT TTTACTGCTG CATTAGCTCA AGTTAGCTTT 
GACAAACTGA AATCAGCAAG GGATGTGGTT ACCAGAACAG GATACTCATT GGCTTTGGGA 
TCTCTACATA GATATTTAGG AGGAATAAGT TCTTCTCAAC ATTTGAGTTC TTGTGTTGGA 
ATCCTTTATA CTTTGTCTCA GGACAGTACT TCTCCTGATG TGCAGACCTG GGCACTACAT 
TCGCTATCAT TGATAATTGA TTCTGCTGGC CCCCTCTATC ATATGCATGT GGAACCCACC 
CTTTCTCTTA TTATAATGTT ACTGTTAAAT GTGCCTCCTA CTCATGCTGA AGTACACCAG 
AGTCTTGGTC GCTGTTTGAA TGCCCTCATT ACTACATTGG GTCCAGAGCT ACAGGGTAAT 
AGTACTTCCA TTTCTACCTT AAGGACTTCC TGTCTTTTGG GTTGTGCAGT GATGCAAGAT 
AACCCAGACT GCCTCGTTCA AGCTCAGGCC ATCTCTTGCC TTCAGCAGCT TCATATGTTT 
GCACCAAGAC ATGTCAACTT GTCCAGCTTG GTCAGCTGCC TCTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT TTGTAGTCCC TATTTGTTAC TGAGAAGAGC AGTACTGGCC 
TGTCTCCGTC AACTTGTACA GAGAGAAGCA GCTGAAGTTT CAGAACATGC TGTTTTGCTT 
GCTAAAGACA ACAGAGAAGA GTTGACTTCA GATGCTAACA TCAGAGAAGT GGGCCTTGAA 
GGGGCATTAT TGACCTTACT AGATAAAGAA ACAGACCAGA AGTTATGCCA TGATATCAAA 
GAGACTCTAA ATCATATGCT TACATCTATG GCAGTGGATA AGCTCTCCTT CTGGTTAAAG 
CTTTGTAAAG ATGTACTTGC TGCATCAGCT GATTTTACAA CTGTGACTTG TGTGGATACA 
ATGCAAGAAG AGGAAGGAGA TAAAGGGGAT GATGCTTCAG TTCTGACCAC TGGAAATGAT 
GACAAGTCTT TTCCTTTCAC CAATCCTCGT TGGGCTACTA GAGTCTTTGC TGCCGAATGT 
GTCTGTAGGA TAATTAACCA GTGTGAGAAT GCACACAGCG CACATTTTGA CATTGCTTTA 
GCACAAGAAA TGAAAAAAAG GGATTCAAGA AATGACTTTT TGGTGCTACA TCTTGCCGAC 
TTAATTCGCA TGGCCTTTAT GGCTGCCACA GATCACAGTG ACCAGCTCCG ACTTTCTGGC 
CTTGAAACAC TATTAGTTGT TATTCGGCGA TTTGCAGCTA TTCCAGAACC AGAGTTTCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTAGGAG CTGCACTTAG GCCAGCCTTC 
ACTTCAGAGA CGCCACCTGA TGTCACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA CCTTAATGAT TTACGAAGAG TTCATCAGTT GCTTGTTTCA 
TCATTAACAA AAATACAGGC TGGAAAAGAG GCTCTGAGTC ACTTATATAA TGAAAGCGCT 
TCTACCATGG AGATCTTAGC TGTGCTGAAA GCCTGGGCAG AGGTCTACAT AATTGCTGTA 
GAAAGACATA AAAGCCACAG ACAACCTTTG AAGACTATCC CCTGTTCAGA AGAGAGTGTC 
AGAAATGGGC CACATTCAAC AGATGGACTG CTTGACTTAG TCCACACAGA CCTGAGCACA 
CTGAGTAGGC TCTGGCTTGC TGCACTTCAG GATTTTGCTC TCTTAACTTT GCCTTCAGAA 
TTTGCCTCCC AACTTCCTGT TGAAGGTGGT GCTTTCTACA CAGCAGAGAC TAGTGAAAAT 
GCAAAATTAC ATTATTACAA CTCCTGGGCG CTTATCCTGC ATGCTACAGC ATTGTGGCTT 
ACCAGCACAG GTTTTGTTGT TGCTGATCCG GATGAAGAAG CAGCTCATCT TTCAAGACCT 
GTAACACCAA CTTCCATGTG TCAGGGTTCA TCATCTGGAG CTACAGTTAA GTCCCCTGAA 
GATGTCTGCA CTGATAGATT CCATCTTATT TTAGGAATAA GTGTGGAGTT TCTGTGTTCC 
TTACGCTCAG ATGCAACCAT GGAAAGCATT ACTGCTTGTT TACATGCCTT ACAGGCCCTT 
CTAGATGTTC CTTGGCCCAG ATCTAAAATT GGCAGTGATC AGGATTTGGG CATCGAGTTG 
TTGAATGTAC TACATCGAGT AATTTTGACC CGAGAATCAC CCTCTATTCA ATTGGCTTCA 
CTTGAAGTGG TAAGACAGAT TATCTGTGCA GCTCAAGAAC ATGTCAAGGA AAAAAGACGA 
AGTGCAGAAG TTGATGATGG GGCTGCCGAA AAGGAAACCC TGCCAGAGTT TGGAGAGGGA 
AAGGACACAG GAGGACTTGT GCCTGGGAAA TCTTTGGTTT TTGCAACACT GGAATTGTGT 
GTATGCATTT TAGTTAGACA GCTTCCAGAA CTAAATCCTA AATTGACAGG CAGCCCAGGA 
GTAAAAGCTA CAAAGCCACA GGTGCTGTCT GAGGATGGAA GTAGATTGGT GTCAGCTGCG 
TTAGTTATCC TCTCTGAACT TCCTGCCGTG TGCTCTCCTG AAGGAAGCAT CTCAATTCTC 
CCTACTATAT TATACCTTAC AATTGGGGTC CTCAGAGAAA CTGCTGTGAA GTTACCTGGA 
GGCCAGTTAC CTTCAACTGT TGCAGCTTCC CTACAGGCTC TGAAAGGAAT ATTGTCTTCT 
CCCATGGCCA GAGCAGAAAA GAGTCACAGT GCTTGGACTG ACCTCCTCCG TAGTGCTTTA 
ACAACAATAC TTGACTGTTG GGATCCAGAT CATTTCTCTT TAGTAGATGG AACACATCAA 
GAACTTGATG AAATCAGTCT ACTTACTGCT ATCACAGTAT TTATTTTGTC TACCAGTCCA 
GAAGTTACTA CCATCCCATG CCTTCAGAAG CGCTGTATTG AGAAATTTAA GGCTACTCTT 
GAGATAAAAG ATCCTGTGGT ACAAATCAAG ACCTATCAGC TACTACTTTC CATTTTTCAG 
TATCCAAATC CAGCTGTTTC TTACCCATAC ATATACTCTT TAGTATCCTC TATTGTGGAA 
AAGCTACAGG AAATAGACAA GAGAAAGCCT GAAGACACTG CTGAGCTTCA GATCTTTCAA 
GAAGGCATAA AGGTCTTGCA AGCACTGGTG GCCATTGCCG AAGAACAACA CCGTTCTCAG 
CTGGTGGCCT GTCTCCTGCC CATCCTCATT TCCTTCCTTT TGGATGATAA TGATCTGGGA 
TCTGCAACTT CTGTAATGAA AAATTTACAT GACTTCGCTT TGCAAAATCT CATGCAGATT 
GGGCCTCAGT ATTCATCAGT TTTTAAAAAT GTAATGGCTT CTTCTCCAGC CCTGAAAGCC 
CGCCTTGAGG CTGCTATCAA GGGCAATCAG GAAAGTGTAA AAATCAAGAT ACCAACATCT 
AAACATACTA AGAACCCTGG AAAGAATACA AGCATCCAAT TAAAGACCAA CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421890.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277598.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X10, mRNA.

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CloneID	OCe290022
Clone ID Related Accession (Same CDS sequence)	XM_022421890.1
Accession Version	XM_022421890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGT GAATCTTTGT 
AGTCCCTATT TGTTACTGAG AAGAGCAGTA CTGGCCTGTC TCCGTCAACT TGTACAGAGA 
GAAGCAGCTG AAGTTTCAGA ACATGCTGTT TTGCTTGCTA AAGACAACAG AGAAGAGTTG 
ACTTCAGATG CTAACATCAG AGAAGTGGGC CTTGAAGGGG CATTATTGAC CTTACTAGAT 
AAAGAAACAG ACCAGAAGTT ATGCCATGAT ATCAAAGAGA CTCTAAATCA TATGCTTACA 
TCTATGGCAG TGGATAAGCT CTCCTTCTGG TTAAAGCTTT GTAAAGATGT ACTTGCTGCA 
TCAGCTGATT TTACAACTGT GACTTGTGTG GATACAATGC AAGAAGAGGA AGGAGATAAA 
GGGGATGATG CTTCAGTTCT GACCACTGGA AATGATGACA AGTCTTTTCC TTTCACCAAT 
CCTCGTTGGG CTACTAGAGT CTTTGCTGCC GAATGTGTCT GTAGGATAAT TAACCAGTGT 
GAGAATGCAC ACAGCGCACA TTTTGACATT GCTTTAGCAC AAGAAATGAA AAAAAGGGAT 
TCAAGAAATG ACTTTTTGGT GCTACATCTT GCCGACTTAA TTCGCATGGC CTTTATGGCT 
GCCACAGATC ACAGTGACCA GCTCCGACTT TCTGGCCTTG AAACACTATT AGTTGTTATT 
CGGCGATTTG CAGCTATTCC AGAACCAGAG TTTCCAGGTC ATGTGATTCT GGAACAGTAT 
CAAGCCAATG TAGGAGCTGC ACTTAGGCCA GCCTTCACTT CAGAGACGCC ACCTGATGTC 
ACTGCCAAAG CATGTCAGGT TTGCAGTGCC TGGATAGCAA GTGGAGTTGT AAGTGACCTT 
AATGATTTAC GAAGAGTTCA TCAGTTGCTT GTTTCATCAT TAACAAAAAT ACAGGCTGGA 
AAAGAGGCTC TGAGTCACTT ATATAATGAA AGCGCTTCTA CCATGGAGAT CTTAGCTGTG 
CTGAAAGCCT GGGCAGAGGT CTACATAATT GCTGTAGAAA GACATAAAAG CCACAGACAA 
CCTTTGAAGA CTATCCCCTG TTCAGAAGAG AGTGTCAGAA ATGGGCCACA TTCAACAGAT 
GGACTGCTTG ACTTAGTCCA CACAGACCTG AGCACACTGA GTAGGCTCTG GCTTGCTGCA 
CTTCAGGATT TTGCTCTCTT AACTTTGCCT TCAGAATTTG CCTCCCAACT TCCTGTTGAA 
GGTGGTGCTT TCTACACAGC AGAGACTAGT GAAAATGCAA AATTACATTA TTACAACTCC 
TGGGCGCTTA TCCTGCATGC TACAGCATTG TGGCTTACCA GCACAGGTTT TGTTGTTGCT 
GATCCGGATG AAGAAGCAGC TCATCTTTCA AGACCTGTAA CACCAACTTC CATGTGTCAG 
GGTTCATCAT CTGGAGCTAC AGTTAAGTCC CCTGAAGATG TCTGCACTGA TAGATTCCAT 
CTTATTTTAG GAATAAGTGT GGAGTTTCTG TGTTCCTTAC GCTCAGATGC AACCATGGAA 
AGCATTACTG CTTGTTTACA TGCCTTACAG GCCCTTCTAG ATGTTCCTTG GCCCAGATCT 
AAAATTGGCA GTGATCAGGA TTTGGGCATC GAGTTGTTGA ATGTACTACA TCGAGTAATT 
TTGACCCGAG AATCACCCTC TATTCAATTG GCTTCACTTG AAGTGGTAAG ACAGATTATC 
TGTGCAGCTC AAGAACATGT CAAGGAAAAA AGACGAAGTG CAGAAGTTGA TGATGGGGCT 
GCCGAAAAGG AAACCCTGCC AGAGTTTGGA GAGGGAAAGG ACACAGGAGG ACTTGTGCCT 
GGGAAATCTT TGGTTTTTGC AACACTGGAA TTGTGTGTAT GCATTTTAGT TAGACAGCTT 
CCAGAACTAA ATCCTAAATT GACAGGCAGC CCAGGAGTAA AAGCTACAAA GCCACAGGTG 
CTGTCTGAGG ATGGAAGTAG ATTGGTGTCA GCTGCGTTAG TTATCCTCTC TGAACTTCCT 
GCCGTGTGCT CTCCTGAAGG AAGCATCTCA ATTCTCCCTA CTATATTATA CCTTACAATT 
GGGGTCCTCA GAGAAACTGC TGTGAAGTTA CCTGGAGGCC AGTTACCTTC AACTGTTGCA 
GCTTCCCTAC AGGCTCTGAA AGGAATATTG TCTTCTCCCA TGGCCAGAGC AGAAAAGAGT 
CACAGTGCTT GGACTGACCT CCTCCGTAGT GCTTTAACAA CAATACTTGA CTGTTGGGAT 
CCAGATCATT TCTCTTTAGT AGATGGAACA CATCAAGAAC TTGATGAAAT CAGTCTACTT 
ACTGCTATCA CAGTATTTAT TTTGTCTACC AGTCCAGAAG TTACTACCAT CCCATGCCTT 
CAGAAGCGCT GTATTGAGAA ATTTAAGGCT ACTCTTGAGA TAAAAGATCC TGTGGTACAA 
ATCAAGACCT ATCAGCTACT ACTTTCCATT TTTCAGTATC CAAATCCAGC TGTTTCTTAC 
CCATACATAT ACTCTTTAGT ATCCTCTATT GTGGAAAAGC TACAGGAAAT AGACAAGAGA 
AAGCCTGAAG ACACTGCTGA GCTTCAGATC TTTCAAGAAG GCATAAAGGT CTTGCAAGCA 
CTGGTGGCCA TTGCCGAAGA ACAACACCGT TCTCAGCTGG TGGCCTGTCT CCTGCCCATC 
CTCATTTCCT TCCTTTTGGA TGATAATGAT CTGGGATCTG CAACTTCTGT AATGAAAAAT 
TTACATGACT TCGCTTTGCA AAATCTCATG CAGATTGGGC CTCAGTATTC ATCAGTTTTT 
AAAAATGTAA TGGCTTCTTC TCCAGCCCTG AAAGCCCGCC TTGAGGCTGC TATCAAGGGC 
AATCAGGAAA GTGTAAAAAT CAAGATACCA ACATCTAAAC ATACTAAGAA CCCTGGAAAG 
AATACAAGCA TCCAATTAAA GACCAACTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277598.1
CDS	207..6362
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421890.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421890.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGT GAATCTTTGT 
AGTCCCTATT TGTTACTGAG AAGAGCAGTA CTGGCCTGTC TCCGTCAACT TGTACAGAGA 
GAAGCAGCTG AAGTTTCAGA ACATGCTGTT TTGCTTGCTA AAGACAACAG AGAAGAGTTG 
ACTTCAGATG CTAACATCAG AGAAGTGGGC CTTGAAGGGG CATTATTGAC CTTACTAGAT 
AAAGAAACAG ACCAGAAGTT ATGCCATGAT ATCAAAGAGA CTCTAAATCA TATGCTTACA 
TCTATGGCAG TGGATAAGCT CTCCTTCTGG TTAAAGCTTT GTAAAGATGT ACTTGCTGCA 
TCAGCTGATT TTACAACTGT GACTTGTGTG GATACAATGC AAGAAGAGGA AGGAGATAAA 
GGGGATGATG CTTCAGTTCT GACCACTGGA AATGATGACA AGTCTTTTCC TTTCACCAAT 
CCTCGTTGGG CTACTAGAGT CTTTGCTGCC GAATGTGTCT GTAGGATAAT TAACCAGTGT 
GAGAATGCAC ACAGCGCACA TTTTGACATT GCTTTAGCAC AAGAAATGAA AAAAAGGGAT 
TCAAGAAATG ACTTTTTGGT GCTACATCTT GCCGACTTAA TTCGCATGGC CTTTATGGCT 
GCCACAGATC ACAGTGACCA GCTCCGACTT TCTGGCCTTG AAACACTATT AGTTGTTATT 
CGGCGATTTG CAGCTATTCC AGAACCAGAG TTTCCAGGTC ATGTGATTCT GGAACAGTAT 
CAAGCCAATG TAGGAGCTGC ACTTAGGCCA GCCTTCACTT CAGAGACGCC ACCTGATGTC 
ACTGCCAAAG CATGTCAGGT TTGCAGTGCC TGGATAGCAA GTGGAGTTGT AAGTGACCTT 
AATGATTTAC GAAGAGTTCA TCAGTTGCTT GTTTCATCAT TAACAAAAAT ACAGGCTGGA 
AAAGAGGCTC TGAGTCACTT ATATAATGAA AGCGCTTCTA CCATGGAGAT CTTAGCTGTG 
CTGAAAGCCT GGGCAGAGGT CTACATAATT GCTGTAGAAA GACATAAAAG CCACAGACAA 
CCTTTGAAGA CTATCCCCTG TTCAGAAGAG AGTGTCAGAA ATGGGCCACA TTCAACAGAT 
GGACTGCTTG ACTTAGTCCA CACAGACCTG AGCACACTGA GTAGGCTCTG GCTTGCTGCA 
CTTCAGGATT TTGCTCTCTT AACTTTGCCT TCAGAATTTG CCTCCCAACT TCCTGTTGAA 
GGTGGTGCTT TCTACACAGC AGAGACTAGT GAAAATGCAA AATTACATTA TTACAACTCC 
TGGGCGCTTA TCCTGCATGC TACAGCATTG TGGCTTACCA GCACAGGTTT TGTTGTTGCT 
GATCCGGATG AAGAAGCAGC TCATCTTTCA AGACCTGTAA CACCAACTTC CATGTGTCAG 
GGTTCATCAT CTGGAGCTAC AGTTAAGTCC CCTGAAGATG TCTGCACTGA TAGATTCCAT 
CTTATTTTAG GAATAAGTGT GGAGTTTCTG TGTTCCTTAC GCTCAGATGC AACCATGGAA 
AGCATTACTG CTTGTTTACA TGCCTTACAG GCCCTTCTAG ATGTTCCTTG GCCCAGATCT 
AAAATTGGCA GTGATCAGGA TTTGGGCATC GAGTTGTTGA ATGTACTACA TCGAGTAATT 
TTGACCCGAG AATCACCCTC TATTCAATTG GCTTCACTTG AAGTGGTAAG ACAGATTATC 
TGTGCAGCTC AAGAACATGT CAAGGAAAAA AGACGAAGTG CAGAAGTTGA TGATGGGGCT 
GCCGAAAAGG AAACCCTGCC AGAGTTTGGA GAGGGAAAGG ACACAGGAGG ACTTGTGCCT 
GGGAAATCTT TGGTTTTTGC AACACTGGAA TTGTGTGTAT GCATTTTAGT TAGACAGCTT 
CCAGAACTAA ATCCTAAATT GACAGGCAGC CCAGGAGTAA AAGCTACAAA GCCACAGGTG 
CTGTCTGAGG ATGGAAGTAG ATTGGTGTCA GCTGCGTTAG TTATCCTCTC TGAACTTCCT 
GCCGTGTGCT CTCCTGAAGG AAGCATCTCA ATTCTCCCTA CTATATTATA CCTTACAATT 
GGGGTCCTCA GAGAAACTGC TGTGAAGTTA CCTGGAGGCC AGTTACCTTC AACTGTTGCA 
GCTTCCCTAC AGGCTCTGAA AGGAATATTG TCTTCTCCCA TGGCCAGAGC AGAAAAGAGT 
CACAGTGCTT GGACTGACCT CCTCCGTAGT GCTTTAACAA CAATACTTGA CTGTTGGGAT 
CCAGATCATT TCTCTTTAGT AGATGGAACA CATCAAGAAC TTGATGAAAT CAGTCTACTT 
ACTGCTATCA CAGTATTTAT TTTGTCTACC AGTCCAGAAG TTACTACCAT CCCATGCCTT 
CAGAAGCGCT GTATTGAGAA ATTTAAGGCT ACTCTTGAGA TAAAAGATCC TGTGGTACAA 
ATCAAGACCT ATCAGCTACT ACTTTCCATT TTTCAGTATC CAAATCCAGC TGTTTCTTAC 
CCATACATAT ACTCTTTAGT ATCCTCTATT GTGGAAAAGC TACAGGAAAT AGACAAGAGA 
AAGCCTGAAG ACACTGCTGA GCTTCAGATC TTTCAAGAAG GCATAAAGGT CTTGCAAGCA 
CTGGTGGCCA TTGCCGAAGA ACAACACCGT TCTCAGCTGG TGGCCTGTCT CCTGCCCATC 
CTCATTTCCT TCCTTTTGGA TGATAATGAT CTGGGATCTG CAACTTCTGT AATGAAAAAT 
TTACATGACT TCGCTTTGCA AAATCTCATG CAGATTGGGC CTCAGTATTC ATCAGTTTTT 
AAAAATGTAA TGGCTTCTTC TCCAGCCCTG AAAGCCCGCC TTGAGGCTGC TATCAAGGGC 
AATCAGGAAA GTGTAAAAAT CAAGATACCA ACATCTAAAC ATACTAAGAA CCCTGGAAAG 
AATACAAGCA TCCAATTAAA GACCAACTTC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_022421892.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277600.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X13, mRNA.

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CloneID	OCe290023
Clone ID Related Accession (Same CDS sequence)	XM_022421892.1
Accession Version	XM_022421892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGTGACAGCC 
AGCCAGCATA TGCTGGTGTG TGCTTTACAA GAACTTGGAA ATCTCATATA CAGTCTTGGC 
ACCACAGCTG CACCTTTACT GCAGGATTCA AGTGCAGGCA TTCTTGATAG TGTCATTTCA 
GTTATTCTAC ATCCTAGCAT TTCTGTTCGA CTAGCAACGG CTTGGTGTTT ACACTGCATT 
GCTGTGGCAT TACCTTCTTA CCTAACACCT CTTTTAGACC GGTGCCTGGA ACGGCTTACT 
GTACTTAAGT CTTCACCGGA AGCAGTGACT GGCTTTAGTT TTGCTGTGGC AGCTTTGTTG 
GGAGCAGTGA AACATTGTCC TTTAGGAATT CCTCATGGGA AAGGCAAGAT TATTATGACT 
TTAGCAGAAG ATTTACTGTG TTCAGCTGTA CAAAACAGTC GTCTTTCAGC TCAGCGCACA 
CAAGCTGGTT GGTTGTTGAT TGCTGCTCTG ATGACATTAG GTCCTGCAGT TGTCAGTCAT 
CACCTTGCTC GACTTCTGCT ATTGTGGAAG TGTGTCTTTC CAGTGTCTCC TAAAGATCTA 
GAAACAGAAA AGAGCCGAGG AGATTCATTT ACCTGGCAGG TAACCTTGGA AGGACGAGCT 
GGTGCGCTCT GTGCTGTCAA GAGTTTTGTT TCCCACTGTG GTGACCTTCT TACTGAGGAA 
GTAATTCAAC GTCTTCTTCC ACCACTTCCT TGTGCTGTGG ATTTGCTAAC TCAGCTTTCT 
TCAGTACTAA AAACATACGG AAGCACTTTG AAAATGCCAT CAGTAGTTTA TAGACAAAGG 
CTTTATGAAT TACTGATTTT ATTACCTCCT GAAACCTATG AAGAAAACTT CTGTGCTATC 
CTCAAAGAGC TGGCTGCTGA CTTGACTGCC TCTGATATAC AGGTGGCAGC ATCTGCATTT 
TTACTTCCAC CCCTCTGTCA TCAGGATGAT CTTGTGATAT TAAGTCCTTT GCTACAAGAA 
ACTGATCATA GATTTATTGA AGAACAGCTC CTGCTTGGTA ATGGTATTGC TTGTGGAAGT 
TTGGAGTATG ACCCTTATTC TATTTATGAG AAAGATGTAG AGGGAGATTC AGTGCCTAAA 
CCCCTGTCTC CAGTACTTTC AGTTATTAGC TCTGCAGTCA AGCTCTTTGG AGTTATATGT 
GCTCATGTGG GAGAAGCTCA AAGGCTTTCT ATACTGGAAC AGCTTTTGGA CAGTATAAAG 
CACACAAAAG GAGCTCGTCA ACAAGTAGTT CAGTTACATG TTGTTTCTTC ACTCTCTAGT 
TTCTTGAAGT ACTTAGCTGG CTCCAAGGGA AACTTGGGTT CAGAAGAAAT GAGAAAACCT 
GCCCTAGCAT TGGTGATGGG AGCCCTAGAA AGTCCCAACC CCCTCTTGAG ATGTGCAGCT 
GCAGAGGCAT GGGCTAGATT AGCCCAAGTG GTTAATGATG GAGCTTTTAC TGCTGCATTA 
GCTCAAGTTA GCTTTGACAA ACTGAAATCA GCAAGGGATG TGGTTACCAG AACAGGATAC 
TCATTGGCTT TGGGATCTCT ACATAGATAT TTAGGAGGAA TAAGTTCTTC TCAACATTTG 
AGTTCTTGTG TTGGAATCCT TTATACTTTG TCTCAGGACA GTACTTCTCC TGATGTGCAG 
ACCTGGGCAC TACATTCGCT ATCATTGATA ATTGATTCTG CTGGCCCCCT CTATCATATG 
CATGTGGAAC CCACCCTTTC TCTTATTATA ATGTTACTGT TAAATGTGCC TCCTACTCAT 
GCTGAAGTAC ACCAGAGTCT TGGTCGCTGT TTGAATGCCC TCATTACTAC ATTGGGTCCA 
GAGCTACAGG GTAATAGTAC TTCCATTTCT ACCTTAAGGA CTTCCTGTCT TTTGGGTTGT 
GCAGTGATGC AAGATAACCC AGACTGCCTC GTTCAAGCTC AGGCCATCTC TTGCCTTCAG 
CAGCTTCATA TGTTTGCACC AAGACATGTC AACTTGTCCA GCTTGGTCAG CTGCCTCTGT 
GAGATCTTGT TGGATAATTC TGTGTTGGTG AATCTTTGTA GTCCCTATTT GTTACTGAGA 
AGAGCAGTAC TGGCCTGTCT CCGTCAACTT GTACAGAGAG AAGCAGCTGA AGTTTCAGAA 
CATGCTGTTT TGCTTGCTAA AGACAACAGA GAAGAGTTGA CTTCAGATGC TAACATCAGA 
GAAGTGGGCC TTGAAGGGGC ATTATTGACC TTACTAGATA AAGAAACAGA CCAGAAGTTA 
TGCCATGATA TCAAAGAGAC TCTAAATCAT ATGCTTACAT CTATGGCAGT GGATAAGCTC 
TCCTTCTGGT TAAAGCTTTG TAAAGATGTA CTTGCTGCAT CAGCTGATTT TACAACTGTG 
ACTTGTGTGG ATACAATGCA AGAAGAGGAA GGAGATAAAG GGGATGATGC TTCAGTTCTG 
ACCACTGGAA ATGATGACAA GTCTTTTCCT TTCACCAATC CTCGTTGGGC TACTAGAGTC 
TTTGCTGCCG AATGTGTCTG TAGGATAATT AACCAGTGTG AGAATGCACA CAGCGCACAT 
TTTGACATTG CTTTAGCACA AGAAATGAAA AAAAGGGATT CAAGAAATGA CTTTTTGGTG 
CTACATCTTG CCGACTTAAT TCGCATGGCC TTTATGGCTG CCACAGATCA CAGTGACCAG 
CTCCGACTTT CTGGCCTTGA AACACTATTA GTTGTTATTC GGCGATTTGC AGCTATTCCA 
GAACCAGAGT TTCCAGGTCA TGTGATTCTG GAACAGTATC AAGCCAATGT AGGAGCTGCA 
CTTAGGCCAG CCTTCACTTC AGAGACGCCA CCTGATGTCA CTGCCAAAGC ATGTCAGGTT 
TGCAGTGCCT GGATAGCAAG TGGAGTTGTA AGTGACCTTA ATGATTTACG AAGAGTTCAT 
CAGTTGCTTG TTTCATCATT AACAAAAATA CAGGCTGGAA AAGAGGCTCT GAGTCACTTA 
TATAATGAAA GCGCTTCTAC CATGGAGATC TTAGCTGTGC TGAAAGCCTG GGCAGAGGTC 
TACATAATTG CTGTAGAAAG ACATAAAAGC CACAGACAAC CTTTGAAGAC TATCCCCTGT 
TCAGAAGAGA GTGTCAGAAA TGGGCCACAT TCAACAGATG GACTGCTTGA CTTAGTCCAC 
ACAGACCTGA GCACACTGAG TAGGCTCTGG CTTGCTGCAC TTCAGGATTT TGCTCTCTTA 
ACTTTGCCTT CAGAATTTGC CTCCCAACTT CCTGTTGAAG GTGGTGCTTT CTACACAGCA 
GAGACTAGTG AAAATGCAAA ATTACATTAT TACAACTCCT GGGCGCTTAT CCTGCATGCT 
ACAGCATTGT GGCTTACCAG CACAGGTTTT GTTGTTGCTG ATCCGGATGA AGAAGCAGCT 
CATCTTTCAA GACCTGTAAC ACCAACTTCC ATGTGTCAGG GTTCATCATC TGGAGCTACA 
GTTAAGTCCC CTGAAGATGT CTGCACTGAT AGATTCCATC TTATTTTAGG AATAAGTGTG 
GAGTTTCTGT GTTCCTTACG CTCAGATGCA ACCATGGAAA GCATTACTGC TTGTTTACAT 
GCCTTACAGG CCCTTCTAGA TGTTCCTTGG CCCAGATCTA AAATTGGCAG TGATCAGGAT 
TTGGGCATCG AGTTGTTGAA TGTACTACAT CGAGTAATTT TGACCCGAGA ATCACCCTCT 
ATTCAATTGG CTTCACTTGA AGTGGTAAGA CAGATTATCT GTGCAGCTCA AGAACATGTC 
AAGGAAAAAA GACGAAGTGC AGAAGTTGAT GATGGGGCTG CCGAAAAGGA AACCCTGCCA 
GAGTTTGGAG AGGGAAAGGA CACAGGAGGA CTTGTGCCTG GGAAATCTTT GGTTTTTGCA 
ACACTGGAAT TGTGTGTATG CATTTTAGTT AGACAGCTTC CAGAACTAAA TCCTAAATTG 
ACAGGCAGCC CAGGAGTAAA AGCTACAAAG CCACAGGTGC TGTCTGAGGA TGGAAGTAGA 
TTGGTGTCAG CTGCGTTAGT TATCCTCTCT GAACTTCCTG CCGTGTGCTC TCCTGAAGGA 
AGCATCTCAA TTCTCCCTAC TATATTATAC CTTACAATTG GGGTCCTCAG AGAAACTGCT 
GTGAAGTTAC CTGGAGGCCA GTTACCTTCA ACTGTTGCAG CTTCCCTACA GGCTCTGAAA 
GGAATATTGT CTTCTCCCAT GGCCAGAGCA GAAAAGAGTC ACAGTGCTTG GACTGACCTC 
CTCCGTAGTG CTTTAACAAC AATACTTGAC TGTTGGGATC CAGATCATTT CTCTTTAGTA 
GATGGAACAC ATCAAGAACT TGATGAAATC AGTCTACTTA CTGCTATCAC AGTATTTATT 
TTGTCTACCA GTCCAGAAGT TACTACCATC CCATGCCTTC AGAAGCGCTG TATTGAGAAA 
TTTAAGGCTA CTCTTGAGAT AAAAGATCCT GTGGTACAAA TCAAGACCTA TCAGCTACTA 
CTTTCCATTT TTCAGTATCC AAATCCAGCT GTTTCTTACC CATACATATA CTCTTTAGTA 
TCCTCTATTG TGGAAAAGCT ACAGGAAATA GACAAGAGAA AGCCTGAAGA CACTGCTGAG 
CTTCAGATCT TTCAAGAAGG CATAAAGGTC TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA 
CAACACCGTT CTCAGCTGGT GGCCTGTCTC CTGCCCATCC TCATTTCCTT CCTTTTGGAT 
GATAATGATC TGGGATCTGC AACTTCTGTA ATGAAAAATT TACATGACTT CGCTTTGCAA 
AATCTCATGC AGATTGGGCC TCAGTATTCA TCAGTTTTTA AAAATGTAAT GGCTTCTTCT 
CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT ATCAAGGGCA ATCAGGAAAG TGTAAAAATC 
AAGATACCAA CATCTAAACA TACTAAGAAC CCTGGAAAGA ATACAAGCAT CCAATTAAAG 
ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277600.1
CDS	207..6341
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDHFSLVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_022421892.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421892.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGTGACAGCC 
AGCCAGCATA TGCTGGTGTG TGCTTTACAA GAACTTGGAA ATCTCATATA CAGTCTTGGC 
ACCACAGCTG CACCTTTACT GCAGGATTCA AGTGCAGGCA TTCTTGATAG TGTCATTTCA 
GTTATTCTAC ATCCTAGCAT TTCTGTTCGA CTAGCAACGG CTTGGTGTTT ACACTGCATT 
GCTGTGGCAT TACCTTCTTA CCTAACACCT CTTTTAGACC GGTGCCTGGA ACGGCTTACT 
GTACTTAAGT CTTCACCGGA AGCAGTGACT GGCTTTAGTT TTGCTGTGGC AGCTTTGTTG 
GGAGCAGTGA AACATTGTCC TTTAGGAATT CCTCATGGGA AAGGCAAGAT TATTATGACT 
TTAGCAGAAG ATTTACTGTG TTCAGCTGTA CAAAACAGTC GTCTTTCAGC TCAGCGCACA 
CAAGCTGGTT GGTTGTTGAT TGCTGCTCTG ATGACATTAG GTCCTGCAGT TGTCAGTCAT 
CACCTTGCTC GACTTCTGCT ATTGTGGAAG TGTGTCTTTC CAGTGTCTCC TAAAGATCTA 
GAAACAGAAA AGAGCCGAGG AGATTCATTT ACCTGGCAGG TAACCTTGGA AGGACGAGCT 
GGTGCGCTCT GTGCTGTCAA GAGTTTTGTT TCCCACTGTG GTGACCTTCT TACTGAGGAA 
GTAATTCAAC GTCTTCTTCC ACCACTTCCT TGTGCTGTGG ATTTGCTAAC TCAGCTTTCT 
TCAGTACTAA AAACATACGG AAGCACTTTG AAAATGCCAT CAGTAGTTTA TAGACAAAGG 
CTTTATGAAT TACTGATTTT ATTACCTCCT GAAACCTATG AAGAAAACTT CTGTGCTATC 
CTCAAAGAGC TGGCTGCTGA CTTGACTGCC TCTGATATAC AGGTGGCAGC ATCTGCATTT 
TTACTTCCAC CCCTCTGTCA TCAGGATGAT CTTGTGATAT TAAGTCCTTT GCTACAAGAA 
ACTGATCATA GATTTATTGA AGAACAGCTC CTGCTTGGTA ATGGTATTGC TTGTGGAAGT 
TTGGAGTATG ACCCTTATTC TATTTATGAG AAAGATGTAG AGGGAGATTC AGTGCCTAAA 
CCCCTGTCTC CAGTACTTTC AGTTATTAGC TCTGCAGTCA AGCTCTTTGG AGTTATATGT 
GCTCATGTGG GAGAAGCTCA AAGGCTTTCT ATACTGGAAC AGCTTTTGGA CAGTATAAAG 
CACACAAAAG GAGCTCGTCA ACAAGTAGTT CAGTTACATG TTGTTTCTTC ACTCTCTAGT 
TTCTTGAAGT ACTTAGCTGG CTCCAAGGGA AACTTGGGTT CAGAAGAAAT GAGAAAACCT 
GCCCTAGCAT TGGTGATGGG AGCCCTAGAA AGTCCCAACC CCCTCTTGAG ATGTGCAGCT 
GCAGAGGCAT GGGCTAGATT AGCCCAAGTG GTTAATGATG GAGCTTTTAC TGCTGCATTA 
GCTCAAGTTA GCTTTGACAA ACTGAAATCA GCAAGGGATG TGGTTACCAG AACAGGATAC 
TCATTGGCTT TGGGATCTCT ACATAGATAT TTAGGAGGAA TAAGTTCTTC TCAACATTTG 
AGTTCTTGTG TTGGAATCCT TTATACTTTG TCTCAGGACA GTACTTCTCC TGATGTGCAG 
ACCTGGGCAC TACATTCGCT ATCATTGATA ATTGATTCTG CTGGCCCCCT CTATCATATG 
CATGTGGAAC CCACCCTTTC TCTTATTATA ATGTTACTGT TAAATGTGCC TCCTACTCAT 
GCTGAAGTAC ACCAGAGTCT TGGTCGCTGT TTGAATGCCC TCATTACTAC ATTGGGTCCA 
GAGCTACAGG GTAATAGTAC TTCCATTTCT ACCTTAAGGA CTTCCTGTCT TTTGGGTTGT 
GCAGTGATGC AAGATAACCC AGACTGCCTC GTTCAAGCTC AGGCCATCTC TTGCCTTCAG 
CAGCTTCATA TGTTTGCACC AAGACATGTC AACTTGTCCA GCTTGGTCAG CTGCCTCTGT 
GAGATCTTGT TGGATAATTC TGTGTTGGTG AATCTTTGTA GTCCCTATTT GTTACTGAGA 
AGAGCAGTAC TGGCCTGTCT CCGTCAACTT GTACAGAGAG AAGCAGCTGA AGTTTCAGAA 
CATGCTGTTT TGCTTGCTAA AGACAACAGA GAAGAGTTGA CTTCAGATGC TAACATCAGA 
GAAGTGGGCC TTGAAGGGGC ATTATTGACC TTACTAGATA AAGAAACAGA CCAGAAGTTA 
TGCCATGATA TCAAAGAGAC TCTAAATCAT ATGCTTACAT CTATGGCAGT GGATAAGCTC 
TCCTTCTGGT TAAAGCTTTG TAAAGATGTA CTTGCTGCAT CAGCTGATTT TACAACTGTG 
ACTTGTGTGG ATACAATGCA AGAAGAGGAA GGAGATAAAG GGGATGATGC TTCAGTTCTG 
ACCACTGGAA ATGATGACAA GTCTTTTCCT TTCACCAATC CTCGTTGGGC TACTAGAGTC 
TTTGCTGCCG AATGTGTCTG TAGGATAATT AACCAGTGTG AGAATGCACA CAGCGCACAT 
TTTGACATTG CTTTAGCACA AGAAATGAAA AAAAGGGATT CAAGAAATGA CTTTTTGGTG 
CTACATCTTG CCGACTTAAT TCGCATGGCC TTTATGGCTG CCACAGATCA CAGTGACCAG 
CTCCGACTTT CTGGCCTTGA AACACTATTA GTTGTTATTC GGCGATTTGC AGCTATTCCA 
GAACCAGAGT TTCCAGGTCA TGTGATTCTG GAACAGTATC AAGCCAATGT AGGAGCTGCA 
CTTAGGCCAG CCTTCACTTC AGAGACGCCA CCTGATGTCA CTGCCAAAGC ATGTCAGGTT 
TGCAGTGCCT GGATAGCAAG TGGAGTTGTA AGTGACCTTA ATGATTTACG AAGAGTTCAT 
CAGTTGCTTG TTTCATCATT AACAAAAATA CAGGCTGGAA AAGAGGCTCT GAGTCACTTA 
TATAATGAAA GCGCTTCTAC CATGGAGATC TTAGCTGTGC TGAAAGCCTG GGCAGAGGTC 
TACATAATTG CTGTAGAAAG ACATAAAAGC CACAGACAAC CTTTGAAGAC TATCCCCTGT 
TCAGAAGAGA GTGTCAGAAA TGGGCCACAT TCAACAGATG GACTGCTTGA CTTAGTCCAC 
ACAGACCTGA GCACACTGAG TAGGCTCTGG CTTGCTGCAC TTCAGGATTT TGCTCTCTTA 
ACTTTGCCTT CAGAATTTGC CTCCCAACTT CCTGTTGAAG GTGGTGCTTT CTACACAGCA 
GAGACTAGTG AAAATGCAAA ATTACATTAT TACAACTCCT GGGCGCTTAT CCTGCATGCT 
ACAGCATTGT GGCTTACCAG CACAGGTTTT GTTGTTGCTG ATCCGGATGA AGAAGCAGCT 
CATCTTTCAA GACCTGTAAC ACCAACTTCC ATGTGTCAGG GTTCATCATC TGGAGCTACA 
GTTAAGTCCC CTGAAGATGT CTGCACTGAT AGATTCCATC TTATTTTAGG AATAAGTGTG 
GAGTTTCTGT GTTCCTTACG CTCAGATGCA ACCATGGAAA GCATTACTGC TTGTTTACAT 
GCCTTACAGG CCCTTCTAGA TGTTCCTTGG CCCAGATCTA AAATTGGCAG TGATCAGGAT 
TTGGGCATCG AGTTGTTGAA TGTACTACAT CGAGTAATTT TGACCCGAGA ATCACCCTCT 
ATTCAATTGG CTTCACTTGA AGTGGTAAGA CAGATTATCT GTGCAGCTCA AGAACATGTC 
AAGGAAAAAA GACGAAGTGC AGAAGTTGAT GATGGGGCTG CCGAAAAGGA AACCCTGCCA 
GAGTTTGGAG AGGGAAAGGA CACAGGAGGA CTTGTGCCTG GGAAATCTTT GGTTTTTGCA 
ACACTGGAAT TGTGTGTATG CATTTTAGTT AGACAGCTTC CAGAACTAAA TCCTAAATTG 
ACAGGCAGCC CAGGAGTAAA AGCTACAAAG CCACAGGTGC TGTCTGAGGA TGGAAGTAGA 
TTGGTGTCAG CTGCGTTAGT TATCCTCTCT GAACTTCCTG CCGTGTGCTC TCCTGAAGGA 
AGCATCTCAA TTCTCCCTAC TATATTATAC CTTACAATTG GGGTCCTCAG AGAAACTGCT 
GTGAAGTTAC CTGGAGGCCA GTTACCTTCA ACTGTTGCAG CTTCCCTACA GGCTCTGAAA 
GGAATATTGT CTTCTCCCAT GGCCAGAGCA GAAAAGAGTC ACAGTGCTTG GACTGACCTC 
CTCCGTAGTG CTTTAACAAC AATACTTGAC TGTTGGGATC CAGATCATTT CTCTTTAGTA 
GATGGAACAC ATCAAGAACT TGATGAAATC AGTCTACTTA CTGCTATCAC AGTATTTATT 
TTGTCTACCA GTCCAGAAGT TACTACCATC CCATGCCTTC AGAAGCGCTG TATTGAGAAA 
TTTAAGGCTA CTCTTGAGAT AAAAGATCCT GTGGTACAAA TCAAGACCTA TCAGCTACTA 
CTTTCCATTT TTCAGTATCC AAATCCAGCT GTTTCTTACC CATACATATA CTCTTTAGTA 
TCCTCTATTG TGGAAAAGCT ACAGGAAATA GACAAGAGAA AGCCTGAAGA CACTGCTGAG 
CTTCAGATCT TTCAAGAAGG CATAAAGGTC TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA 
CAACACCGTT CTCAGCTGGT GGCCTGTCTC CTGCCCATCC TCATTTCCTT CCTTTTGGAT 
GATAATGATC TGGGATCTGC AACTTCTGTA ATGAAAAATT TACATGACTT CGCTTTGCAA 
AATCTCATGC AGATTGGGCC TCAGTATTCA TCAGTTTTTA AAAATGTAAT GGCTTCTTCT 
CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT ATCAAGGGCA ATCAGGAAAG TGTAAAAATC 
AAGATACCAA CATCTAAACA TACTAAGAAC CCTGGAAAGA ATACAAGCAT CCAATTAAAG 
ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115826.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971301.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971301.1
CDS	247..6414
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115826.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115826.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115827.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971302.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115827.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971302.1
CDS	122..6289
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115827.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115827.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115824.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971299.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115824.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971299.1
CDS	190..6357
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115824.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115824.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115828.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971303.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971303.1
CDS	140..6307
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115828.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115828.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115824.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971299.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X8, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115824.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115824.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971299.1
CDS	207..6374
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115824.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115824.2

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115829.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971304.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X6, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115829.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971304.1
CDS	118..6285
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115829.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115829.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115825.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971300.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

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CloneID	OCe37851
Clone ID Related Accession (Same CDS sequence)	XM_014115826.1 , XM_014115827.1 , XM_014115824.1 , XM_014115828.1 , XM_014115824.2 , XM_014115829.1 , XM_014115825.1
Accession Version	XM_014115825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971300.1
CDS	257..6424
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115825.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115825.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GTAGATGGAA CACATCAAGA ACTTGATGAA 
ATCAGTCTAC TTACTGCTAT CACAGTATTT ATTTTGTCTA CCAGTCCAGA AGTTACTACC 
ATCCCATGCC TTCAGAAGCG CTGTATTGAG AAATTTAAGG CTACTCTTGA GATAAAAGAT 
CCTGTGGTAC AAATCAAGAC CTATCAGCTA CTACTTTCCA TTTTTCAGTA TCCAAATCCA 
GCTGTTTCTT ACCCATACAT ATACTCTTTA GTATCCTCTA TTGTGGAAAA GCTACAGGAA 
ATAGACAAGA GAAAGCCTGA AGACACTGCT GAGCTTCAGA TCTTTCAAGA AGGCATAAAG 
GTCTTGCAAG CACTGGTGGC CATTGCCGAA GAACAACACC GTTCTCAGCT GGTGGCCTGT 
CTCCTGCCCA TCCTCATTTC CTTCCTTTTG GATGATAATG ATCTGGGATC TGCAACTTCT 
GTAATGAAAA ATTTACATGA CTTCGCTTTG CAAAATCTCA TGCAGATTGG GCCTCAGTAT 
TCATCAGTTT TTAAAAATGT AATGGCTTCT TCTCCAGCCC TGAAAGCCCG CCTTGAGGCT 
GCTATCAAGG GCAATCAGGA AAGTGTAAAA ATCAAGATAC CAACATCTAA ACATACTAAG 
AACCCTGGAA AGAATACAAG CATCCAATTA AAGACCAACT TCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115830.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971305.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X7, mRNA.

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CloneID	OCe37852
Clone ID Related Accession (Same CDS sequence)	XM_014115830.1
Accession Version	XM_014115830.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGTCTCACGT 
CAAAGCATTC GCAGAGTATC TCTGGAGGAA GTTCTGGAAT TACTAGGAAC AGGGTTTCTA 
CGTGGAAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGC TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG AGTTACTCAG GCTTATGTGG TATTTGTTTC AACATTAGGA 
GGAACATGGC TAGAGAAAAA TTTTGCTACC TTTCTTTCTC ATATCCTAAG CCTTGTGTCA 
CAGTCGCACC CCAAAGCCAC CCAAACTCAG ACTGATGCTG TCTGCGGTCG CCGCTGTGTT 
TCATTTATCC TTCGAGCTAC TATAGGAGGT CTTCTTGGAG AAAAGGCCCA AATTGCTGCT 
GCCAAGGATA TTTGCCAGGC TATCTGGAAG TTAAAGAAAG TTGTGGATGC TGTGATGAAT 
GACGGTAATA TGGAAATCCG GCTTGGCTCC ACAGATGTGA CAGCCAGCCA GCATATGCTG 
GTGTGTGCTT TACAAGAACT TGGAAATCTC ATATACAGTC TTGGCACCAC AGCTGCACCT 
TTACTGCAGG ATTCAAGTGC AGGCATTCTT GATAGTGTCA TTTCAGTTAT TCTACATCCT 
AGCATTTCTG TTCGACTAGC AACGGCTTGG TGTTTACACT GCATTGCTGT GGCATTACCT 
TCTTACCTAA CACCTCTTTT AGACCGGTGC CTGGAACGGC TTACTGTACT TAAGTCTTCA 
CCGGAAGCAG TGACTGGCTT TAGTTTTGCT GTGGCAGCTT TGTTGGGAGC AGTGAAACAT 
TGTCCTTTAG GAATTCCTCA TGGGAAAGGC AAGATTATTA TGACTTTAGC AGAAGATTTA 
CTGTGTTCAG CTGTACAAAA CAGTCGTCTT TCAGCTCAGC GCACACAAGC TGGTTGGTTG 
TTGATTGCTG CTCTGATGAC ATTAGGTCCT GCAGTTGTCA GTCATCACCT TGCTCGACTT 
CTGCTATTGT GGAAGTGTGT CTTTCCAGTG TCTCCTAAAG ATCTAGAAAC AGAAAAGAGC 
CGAGGAGATT CATTTACCTG GCAGGTAACC TTGGAAGGAC GAGCTGGTGC GCTCTGTGCT 
GTCAAGAGTT TTGTTTCCCA CTGTGGTGAC CTTCTTACTG AGGAAGTAAT TCAACGTCTT 
CTTCCACCAC TTCCTTGTGC TGTGGATTTG CTAACTCAGC TTTCTTCAGT ACTAAAAACA 
TACGGAAGCA CTTTGAAAAT GCCATCAGTA GTTTATAGAC AAAGGCTTTA TGAATTACTG 
ATTTTATTAC CTCCTGAAAC CTATGAAGAA AACTTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACTTGA CTGCCTCTGA TATACAGGTG GCAGCATCTG CATTTTTACT TCCACCCCTC 
TGTCATCAGG ATGATCTTGT GATATTAAGT CCTTTGCTAC AAGAAACTGA TCATAGATTT 
ATTGAAGAAC AGCTCCTGCT TGGTAATGGT ATTGCTTGTG GAAGTTTGGA GTATGACCCT 
TATTCTATTT ATGAGAAAGA TGTAGAGGGA GATTCAGTGC CTAAACCCCT GTCTCCAGTA 
CTTTCAGTTA TTAGCTCTGC AGTCAAGCTC TTTGGAGTTA TATGTGCTCA TGTGGGAGAA 
GCTCAAAGGC TTTCTATACT GGAACAGCTT TTGGACAGTA TAAAGCACAC AAAAGGAGCT 
CGTCAACAAG TAGTTCAGTT ACATGTTGTT TCTTCACTCT CTAGTTTCTT GAAGTACTTA 
GCTGGCTCCA AGGGAAACTT GGGTTCAGAA GAAATGAGAA AACCTGCCCT AGCATTGGTG 
ATGGGAGCCC TAGAAAGTCC CAACCCCCTC TTGAGATGTG CAGCTGCAGA GGCATGGGCT 
AGATTAGCCC AAGTGGTTAA TGATGGAGCT TTTACTGCTG CATTAGCTCA AGTTAGCTTT 
GACAAACTGA AATCAGCAAG GGATGTGGTT ACCAGAACAG GATACTCATT GGCTTTGGGA 
TCTCTACATA GATATTTAGG AGGAATAAGT TCTTCTCAAC ATTTGAGTTC TTGTGTTGGA 
ATCCTTTATA CTTTGTCTCA GGACAGTACT TCTCCTGATG TGCAGACCTG GGCACTACAT 
TCGCTATCAT TGATAATTGA TTCTGCTGGC CCCCTCTATC ATATGCATGT GGAACCCACC 
CTTTCTCTTA TTATAATGTT ACTGTTAAAT GTGCCTCCTA CTCATGCTGA AGTACACCAG 
AGTCTTGGTC GCTGTTTGAA TGCCCTCATT ACTACATTGG GTCCAGAGCT ACAGGGTAAT 
AGTACTTCCA TTTCTACCTT AAGGACTTCC TGTCTTTTGG GTTGTGCAGT GATGCAAGAT 
AACCCAGACT GCCTCGTTCA AGCTCAGGCC ATCTCTTGCC TTCAGCAGCT TCATATGTTT 
GCACCAAGAC ATGTCAACTT GTCCAGCTTG GTCAGCTGCC TCTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT TTGTAGTCCC TATTTGTTAC TGAGAAGAGC AGTACTGGCC 
TGTCTCCGTC AACTTGTACA GAGAGAAGCA GCTGAAGTTT CAGAACATGC TGTTTTGCTT 
GCTAAAGACA ACAGAGAAGA GTTGACTTCA GATGCTAACA TCAGAGAAGT GGGCCTTGAA 
GGGGCATTAT TGACCTTACT AGATAAAGAA ACAGACCAGA AGTTATGCCA TGATATCAAA 
GAGACTCTAA ATCATATGCT TACATCTATG GCAGTGGATA AGCTCTCCTT CTGGTTAAAG 
CTTTGTAAAG ATGTACTTGC TGCATCAGCT GATTTTACAA CTGTGACTTG TGTGGATACA 
ATGCAAGAAG AGGAAGGAGA TAAAGGGGAT GATGCTTCAG TTCTGACCAC TGGAAATGAT 
GACAAGTCTT TTCCTTTCAC CAATCCTCGT TGGGCTACTA GAGTCTTTGC TGCCGAATGT 
GTCTGTAGGA TAATTAACCA GTGTGAGAAT GCACACAGCG CACATTTTGA CATTGCTTTA 
GCACAAGAAA TGAAAAAAAG GGATTCAAGA AATGACTTTT TGGTGCTACA TCTTGCCGAC 
TTAATTCGCA TGGCCTTTAT GGCTGCCACA GATCACAGTG ACCAGCTCCG ACTTTCTGGC 
CTTGAAACAC TATTAGTTGT TATTCGGCGA TTTGCAGCTA TTCCAGAACC AGAGTTTCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTAGGAG CTGCACTTAG GCCAGCCTTC 
ACTTCAGAGA CGCCACCTGA TGTCACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA CCTTAATGAT TTACGAAGAG TTCATCAGTT GCTTGTTTCA 
TCATTAACAA AAATACAGGC TGGAAAAGAG GCTCTGAGTC ACTTATATAA TGAAAGCGCT 
TCTACCATGG AGATCTTAGC TGTGCTGAAA GCCTGGGCAG AGGTCTACAT AATTGCTGTA 
GAAAGACATA AAAGCCACAG ACAACCTTTG AAGACTATCC CCTGTTCAGA AGAGAGTGTC 
AGAAATGGGC CACATTCAAC AGATGGACTG CTTGACTTAG TCCACACAGA CCTGAGCACA 
CTGAGTAGGC TCTGGCTTGC TGCACTTCAG GATTTTGCTC TCTTAACTTT GCCTTCAGAA 
TTTGCCTCCC AACTTCCTGT TGAAGGTGGT GCTTTCTACA CAGCAGAGAC TAGTGAAAAT 
GCAAAATTAC ATTATTACAA CTCCTGGGCG CTTATCCTGC ATGCTACAGC ATTGTGGCTT 
ACCAGCACAG GTTTTGTTGT TGCTGATCCG GATGAAGAAG CAGCTCATCT TTCAAGACCT 
GTAACACCAA CTTCCATGTG TCAGGGTTCA TCATCTGGAG CTACAGTTAA GTCCCCTGAA 
GATGTCTGCA CTGATAGATT CCATCTTATT TTAGGAATAA GTGTGGAGTT TCTGTGTTCC 
TTACGCTCAG ATGCAACCAT GGAAAGCATT ACTGCTTGTT TACATGCCTT ACAGGCCCTT 
CTAGATGTTC CTTGGCCCAG ATCTAAAATT GGCAGTGATC AGGATTTGGG CATCGAGTTG 
TTGAATGTAC TACATCGAGT AATTTTGACC CGAGAATCAC CCTCTATTCA ATTGGCTTCA 
CTTGAAGTGG TAAGACAGAT TATCTGTGCA GCTCAAGAAC ATGTCAAGGA AAAAAGACGA 
AGTGCAGAAG TTGATGATGG GGCTGCCGAA AAGGAAACCC TGCCAGAGTT TGGAGAGGGA 
AAGGACACAG GAGGACTTGT GCCTGGGAAA TCTTTGGTTT TTGCAACACT GGAATTGTGT 
GTATGCATTT TAGTTAGACA GCTTCCAGAA CTAAATCCTA AATTGACAGG CAGCCCAGGA 
GTAAAAGCTA CAAAGCCACA GGTGCTGTCT GAGGATGGAA GTAGATTGGT GTCAGCTGCG 
TTAGTTATCC TCTCTGAACT TCCTGCCGTG TGCTCTCCTG AAGGAAGCAT CTCAATTCTC 
CCTACTATAT TATACCTTAC AATTGGGGTC CTCAGAGAAA CTGCTGTGAA GTTACCTGGA 
GGCCAGTTAC CTTCAACTGT TGCAGCTTCC CTACAGGCTC TGAAAGGAAT ATTGTCTTCT 
CCCATGGCCA GAGCAGAAAA GAGTCACAGT GCTTGGACTG ACCTCCTCCG TAGTGCTTTA 
ACAACAATAC TTGACTGTTG GGATCCAGTA GATGGAACAC ATCAAGAACT TGATGAAATC 
AGTCTACTTA CTGCTATCAC AGTATTTATT TTGTCTACCA GTCCAGAAGT TACTACCATC 
CCATGCCTTC AGAAGCGCTG TATTGAGAAA TTTAAGGCTA CTCTTGAGAT AAAAGATCCT 
GTGGTACAAA TCAAGACCTA TCAGCTACTA CTTTCCATTT TTCAGTATCC AAATCCAGCT 
GTTTCTTACC CATACATATA CTCTTTAGTA TCCTCTATTG TGGAAAAGCT ACAGGAAATA 
GACAAGAGAA AGCCTGAAGA CACTGCTGAG CTTCAGATCT TTCAAGAAGG CATAAAGGTC 
TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA CAACACCGTT CTCAGCTGGT GGCCTGTCTC 
CTGCCCATCC TCATTTCCTT CCTTTTGGAT GATAATGATC TGGGATCTGC AACTTCTGTA 
ATGAAAAATT TACATGACTT CGCTTTGCAA AATCTCATGC AGATTGGGCC TCAGTATTCA 
TCAGTTTTTA AAAATGTAAT GGCTTCTTCT CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT 
ATCAAGGGCA ATCAGGAAAG TGTAAAAATC AAGATACCAA CATCTAAACA TACTAAGAAC 
CCTGGAAAGA ATACAAGCAT CCAATTAAAG ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971305.1
CDS	190..6354
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115830.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115830.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGTCTCACGT 
CAAAGCATTC GCAGAGTATC TCTGGAGGAA GTTCTGGAAT TACTAGGAAC AGGGTTTCTA 
CGTGGAAGTT CAGGATTTCT TCGAGCCAGT GGAGATATGC TGAAAGGAAC CAGTTCAGTC 
AGTAGGGATG TTCGAGTTGG AGTTACTCAG GCTTATGTGG TATTTGTTTC AACATTAGGA 
GGAACATGGC TAGAGAAAAA TTTTGCTACC TTTCTTTCTC ATATCCTAAG CCTTGTGTCA 
CAGTCGCACC CCAAAGCCAC CCAAACTCAG ACTGATGCTG TCTGCGGTCG CCGCTGTGTT 
TCATTTATCC TTCGAGCTAC TATAGGAGGT CTTCTTGGAG AAAAGGCCCA AATTGCTGCT 
GCCAAGGATA TTTGCCAGGC TATCTGGAAG TTAAAGAAAG TTGTGGATGC TGTGATGAAT 
GACGGTAATA TGGAAATCCG GCTTGGCTCC ACAGATGTGA CAGCCAGCCA GCATATGCTG 
GTGTGTGCTT TACAAGAACT TGGAAATCTC ATATACAGTC TTGGCACCAC AGCTGCACCT 
TTACTGCAGG ATTCAAGTGC AGGCATTCTT GATAGTGTCA TTTCAGTTAT TCTACATCCT 
AGCATTTCTG TTCGACTAGC AACGGCTTGG TGTTTACACT GCATTGCTGT GGCATTACCT 
TCTTACCTAA CACCTCTTTT AGACCGGTGC CTGGAACGGC TTACTGTACT TAAGTCTTCA 
CCGGAAGCAG TGACTGGCTT TAGTTTTGCT GTGGCAGCTT TGTTGGGAGC AGTGAAACAT 
TGTCCTTTAG GAATTCCTCA TGGGAAAGGC AAGATTATTA TGACTTTAGC AGAAGATTTA 
CTGTGTTCAG CTGTACAAAA CAGTCGTCTT TCAGCTCAGC GCACACAAGC TGGTTGGTTG 
TTGATTGCTG CTCTGATGAC ATTAGGTCCT GCAGTTGTCA GTCATCACCT TGCTCGACTT 
CTGCTATTGT GGAAGTGTGT CTTTCCAGTG TCTCCTAAAG ATCTAGAAAC AGAAAAGAGC 
CGAGGAGATT CATTTACCTG GCAGGTAACC TTGGAAGGAC GAGCTGGTGC GCTCTGTGCT 
GTCAAGAGTT TTGTTTCCCA CTGTGGTGAC CTTCTTACTG AGGAAGTAAT TCAACGTCTT 
CTTCCACCAC TTCCTTGTGC TGTGGATTTG CTAACTCAGC TTTCTTCAGT ACTAAAAACA 
TACGGAAGCA CTTTGAAAAT GCCATCAGTA GTTTATAGAC AAAGGCTTTA TGAATTACTG 
ATTTTATTAC CTCCTGAAAC CTATGAAGAA AACTTCTGTG CTATCCTCAA AGAGCTGGCT 
GCTGACTTGA CTGCCTCTGA TATACAGGTG GCAGCATCTG CATTTTTACT TCCACCCCTC 
TGTCATCAGG ATGATCTTGT GATATTAAGT CCTTTGCTAC AAGAAACTGA TCATAGATTT 
ATTGAAGAAC AGCTCCTGCT TGGTAATGGT ATTGCTTGTG GAAGTTTGGA GTATGACCCT 
TATTCTATTT ATGAGAAAGA TGTAGAGGGA GATTCAGTGC CTAAACCCCT GTCTCCAGTA 
CTTTCAGTTA TTAGCTCTGC AGTCAAGCTC TTTGGAGTTA TATGTGCTCA TGTGGGAGAA 
GCTCAAAGGC TTTCTATACT GGAACAGCTT TTGGACAGTA TAAAGCACAC AAAAGGAGCT 
CGTCAACAAG TAGTTCAGTT ACATGTTGTT TCTTCACTCT CTAGTTTCTT GAAGTACTTA 
GCTGGCTCCA AGGGAAACTT GGGTTCAGAA GAAATGAGAA AACCTGCCCT AGCATTGGTG 
ATGGGAGCCC TAGAAAGTCC CAACCCCCTC TTGAGATGTG CAGCTGCAGA GGCATGGGCT 
AGATTAGCCC AAGTGGTTAA TGATGGAGCT TTTACTGCTG CATTAGCTCA AGTTAGCTTT 
GACAAACTGA AATCAGCAAG GGATGTGGTT ACCAGAACAG GATACTCATT GGCTTTGGGA 
TCTCTACATA GATATTTAGG AGGAATAAGT TCTTCTCAAC ATTTGAGTTC TTGTGTTGGA 
ATCCTTTATA CTTTGTCTCA GGACAGTACT TCTCCTGATG TGCAGACCTG GGCACTACAT 
TCGCTATCAT TGATAATTGA TTCTGCTGGC CCCCTCTATC ATATGCATGT GGAACCCACC 
CTTTCTCTTA TTATAATGTT ACTGTTAAAT GTGCCTCCTA CTCATGCTGA AGTACACCAG 
AGTCTTGGTC GCTGTTTGAA TGCCCTCATT ACTACATTGG GTCCAGAGCT ACAGGGTAAT 
AGTACTTCCA TTTCTACCTT AAGGACTTCC TGTCTTTTGG GTTGTGCAGT GATGCAAGAT 
AACCCAGACT GCCTCGTTCA AGCTCAGGCC ATCTCTTGCC TTCAGCAGCT TCATATGTTT 
GCACCAAGAC ATGTCAACTT GTCCAGCTTG GTCAGCTGCC TCTGTGAGAT CTTGTTGGAT 
AATTCTGTGT TGGTGAATCT TTGTAGTCCC TATTTGTTAC TGAGAAGAGC AGTACTGGCC 
TGTCTCCGTC AACTTGTACA GAGAGAAGCA GCTGAAGTTT CAGAACATGC TGTTTTGCTT 
GCTAAAGACA ACAGAGAAGA GTTGACTTCA GATGCTAACA TCAGAGAAGT GGGCCTTGAA 
GGGGCATTAT TGACCTTACT AGATAAAGAA ACAGACCAGA AGTTATGCCA TGATATCAAA 
GAGACTCTAA ATCATATGCT TACATCTATG GCAGTGGATA AGCTCTCCTT CTGGTTAAAG 
CTTTGTAAAG ATGTACTTGC TGCATCAGCT GATTTTACAA CTGTGACTTG TGTGGATACA 
ATGCAAGAAG AGGAAGGAGA TAAAGGGGAT GATGCTTCAG TTCTGACCAC TGGAAATGAT 
GACAAGTCTT TTCCTTTCAC CAATCCTCGT TGGGCTACTA GAGTCTTTGC TGCCGAATGT 
GTCTGTAGGA TAATTAACCA GTGTGAGAAT GCACACAGCG CACATTTTGA CATTGCTTTA 
GCACAAGAAA TGAAAAAAAG GGATTCAAGA AATGACTTTT TGGTGCTACA TCTTGCCGAC 
TTAATTCGCA TGGCCTTTAT GGCTGCCACA GATCACAGTG ACCAGCTCCG ACTTTCTGGC 
CTTGAAACAC TATTAGTTGT TATTCGGCGA TTTGCAGCTA TTCCAGAACC AGAGTTTCCA 
GGTCATGTGA TTCTGGAACA GTATCAAGCC AATGTAGGAG CTGCACTTAG GCCAGCCTTC 
ACTTCAGAGA CGCCACCTGA TGTCACTGCC AAAGCATGTC AGGTTTGCAG TGCCTGGATA 
GCAAGTGGAG TTGTAAGTGA CCTTAATGAT TTACGAAGAG TTCATCAGTT GCTTGTTTCA 
TCATTAACAA AAATACAGGC TGGAAAAGAG GCTCTGAGTC ACTTATATAA TGAAAGCGCT 
TCTACCATGG AGATCTTAGC TGTGCTGAAA GCCTGGGCAG AGGTCTACAT AATTGCTGTA 
GAAAGACATA AAAGCCACAG ACAACCTTTG AAGACTATCC CCTGTTCAGA AGAGAGTGTC 
AGAAATGGGC CACATTCAAC AGATGGACTG CTTGACTTAG TCCACACAGA CCTGAGCACA 
CTGAGTAGGC TCTGGCTTGC TGCACTTCAG GATTTTGCTC TCTTAACTTT GCCTTCAGAA 
TTTGCCTCCC AACTTCCTGT TGAAGGTGGT GCTTTCTACA CAGCAGAGAC TAGTGAAAAT 
GCAAAATTAC ATTATTACAA CTCCTGGGCG CTTATCCTGC ATGCTACAGC ATTGTGGCTT 
ACCAGCACAG GTTTTGTTGT TGCTGATCCG GATGAAGAAG CAGCTCATCT TTCAAGACCT 
GTAACACCAA CTTCCATGTG TCAGGGTTCA TCATCTGGAG CTACAGTTAA GTCCCCTGAA 
GATGTCTGCA CTGATAGATT CCATCTTATT TTAGGAATAA GTGTGGAGTT TCTGTGTTCC 
TTACGCTCAG ATGCAACCAT GGAAAGCATT ACTGCTTGTT TACATGCCTT ACAGGCCCTT 
CTAGATGTTC CTTGGCCCAG ATCTAAAATT GGCAGTGATC AGGATTTGGG CATCGAGTTG 
TTGAATGTAC TACATCGAGT AATTTTGACC CGAGAATCAC CCTCTATTCA ATTGGCTTCA 
CTTGAAGTGG TAAGACAGAT TATCTGTGCA GCTCAAGAAC ATGTCAAGGA AAAAAGACGA 
AGTGCAGAAG TTGATGATGG GGCTGCCGAA AAGGAAACCC TGCCAGAGTT TGGAGAGGGA 
AAGGACACAG GAGGACTTGT GCCTGGGAAA TCTTTGGTTT TTGCAACACT GGAATTGTGT 
GTATGCATTT TAGTTAGACA GCTTCCAGAA CTAAATCCTA AATTGACAGG CAGCCCAGGA 
GTAAAAGCTA CAAAGCCACA GGTGCTGTCT GAGGATGGAA GTAGATTGGT GTCAGCTGCG 
TTAGTTATCC TCTCTGAACT TCCTGCCGTG TGCTCTCCTG AAGGAAGCAT CTCAATTCTC 
CCTACTATAT TATACCTTAC AATTGGGGTC CTCAGAGAAA CTGCTGTGAA GTTACCTGGA 
GGCCAGTTAC CTTCAACTGT TGCAGCTTCC CTACAGGCTC TGAAAGGAAT ATTGTCTTCT 
CCCATGGCCA GAGCAGAAAA GAGTCACAGT GCTTGGACTG ACCTCCTCCG TAGTGCTTTA 
ACAACAATAC TTGACTGTTG GGATCCAGTA GATGGAACAC ATCAAGAACT TGATGAAATC 
AGTCTACTTA CTGCTATCAC AGTATTTATT TTGTCTACCA GTCCAGAAGT TACTACCATC 
CCATGCCTTC AGAAGCGCTG TATTGAGAAA TTTAAGGCTA CTCTTGAGAT AAAAGATCCT 
GTGGTACAAA TCAAGACCTA TCAGCTACTA CTTTCCATTT TTCAGTATCC AAATCCAGCT 
GTTTCTTACC CATACATATA CTCTTTAGTA TCCTCTATTG TGGAAAAGCT ACAGGAAATA 
GACAAGAGAA AGCCTGAAGA CACTGCTGAG CTTCAGATCT TTCAAGAAGG CATAAAGGTC 
TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA CAACACCGTT CTCAGCTGGT GGCCTGTCTC 
CTGCCCATCC TCATTTCCTT CCTTTTGGAT GATAATGATC TGGGATCTGC AACTTCTGTA 
ATGAAAAATT TACATGACTT CGCTTTGCAA AATCTCATGC AGATTGGGCC TCAGTATTCA 
TCAGTTTTTA AAAATGTAAT GGCTTCTTCT CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT 
ATCAAGGGCA ATCAGGAAAG TGTAAAAATC AAGATACCAA CATCTAAACA TACTAAGAAC 
CCTGGAAAGA ATACAAGCAT CCAATTAAAG ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115831.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971306.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X9, mRNA.

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CloneID	OCe37853
Clone ID Related Accession (Same CDS sequence)	XM_014115831.2 , XM_014115831.1
Accession Version	XM_014115831.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115831.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATGGAACAC ATCAAGAACT TGATGAAATC 
AGTCTACTTA CTGCTATCAC AGTATTTATT TTGTCTACCA GTCCAGAAGT TACTACCATC 
CCATGCCTTC AGAAGCGCTG TATTGAGAAA TTTAAGGCTA CTCTTGAGAT AAAAGATCCT 
GTGGTACAAA TCAAGACCTA TCAGCTACTA CTTTCCATTT TTCAGTATCC AAATCCAGCT 
GTTTCTTACC CATACATATA CTCTTTAGTA TCCTCTATTG TGGAAAAGCT ACAGGAAATA 
GACAAGAGAA AGCCTGAAGA CACTGCTGAG CTTCAGATCT TTCAAGAAGG CATAAAGGTC 
TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA CAACACCGTT CTCAGCTGGT GGCCTGTCTC 
CTGCCCATCC TCATTTCCTT CCTTTTGGAT GATAATGATC TGGGATCTGC AACTTCTGTA 
ATGAAAAATT TACATGACTT CGCTTTGCAA AATCTCATGC AGATTGGGCC TCAGTATTCA 
TCAGTTTTTA AAAATGTAAT GGCTTCTTCT CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT 
ATCAAGGGCA ATCAGGAAAG TGTAAAAATC AAGATACCAA CATCTAAACA TACTAAGAAC 
CCTGGAAAGA ATACAAGCAT CCAATTAAAG ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971306.1
CDS	207..6371
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115831.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115831.2

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATGGAACAC ATCAAGAACT TGATGAAATC 
AGTCTACTTA CTGCTATCAC AGTATTTATT TTGTCTACCA GTCCAGAAGT TACTACCATC 
CCATGCCTTC AGAAGCGCTG TATTGAGAAA TTTAAGGCTA CTCTTGAGAT AAAAGATCCT 
GTGGTACAAA TCAAGACCTA TCAGCTACTA CTTTCCATTT TTCAGTATCC AAATCCAGCT 
GTTTCTTACC CATACATATA CTCTTTAGTA TCCTCTATTG TGGAAAAGCT ACAGGAAATA 
GACAAGAGAA AGCCTGAAGA CACTGCTGAG CTTCAGATCT TTCAAGAAGG CATAAAGGTC 
TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA CAACACCGTT CTCAGCTGGT GGCCTGTCTC 
CTGCCCATCC TCATTTCCTT CCTTTTGGAT GATAATGATC TGGGATCTGC AACTTCTGTA 
ATGAAAAATT TACATGACTT CGCTTTGCAA AATCTCATGC AGATTGGGCC TCAGTATTCA 
TCAGTTTTTA AAAATGTAAT GGCTTCTTCT CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT 
ATCAAGGGCA ATCAGGAAAG TGTAAAAATC AAGATACCAA CATCTAAACA TACTAAGAAC 
CCTGGAAAGA ATACAAGCAT CCAATTAAAG ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_014115831.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971306.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X8, mRNA.

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CloneID	OCe37853
Clone ID Related Accession (Same CDS sequence)	XM_014115831.2 , XM_014115831.1
Accession Version	XM_014115831.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6165bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATGGAACAC ATCAAGAACT TGATGAAATC 
AGTCTACTTA CTGCTATCAC AGTATTTATT TTGTCTACCA GTCCAGAAGT TACTACCATC 
CCATGCCTTC AGAAGCGCTG TATTGAGAAA TTTAAGGCTA CTCTTGAGAT AAAAGATCCT 
GTGGTACAAA TCAAGACCTA TCAGCTACTA CTTTCCATTT TTCAGTATCC AAATCCAGCT 
GTTTCTTACC CATACATATA CTCTTTAGTA TCCTCTATTG TGGAAAAGCT ACAGGAAATA 
GACAAGAGAA AGCCTGAAGA CACTGCTGAG CTTCAGATCT TTCAAGAAGG CATAAAGGTC 
TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA CAACACCGTT CTCAGCTGGT GGCCTGTCTC 
CTGCCCATCC TCATTTCCTT CCTTTTGGAT GATAATGATC TGGGATCTGC AACTTCTGTA 
ATGAAAAATT TACATGACTT CGCTTTGCAA AATCTCATGC AGATTGGGCC TCAGTATTCA 
TCAGTTTTTA AAAATGTAAT GGCTTCTTCT CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT 
ATCAAGGGCA ATCAGGAAAG TGTAAAAATC AAGATACCAA CATCTAAACA TACTAAGAAC 
CCTGGAAAGA ATACAAGCAT CCAATTAAAG ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971306.1
CDS	190..6354
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_014115831.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115831.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGA GATCTTGTTG 
GATAATTCTG TGTTGGTGAA TCTTTGTAGT CCCTATTTGT TACTGAGAAG AGCAGTACTG 
GCCTGTCTCC GTCAACTTGT ACAGAGAGAA GCAGCTGAAG TTTCAGAACA TGCTGTTTTG 
CTTGCTAAAG ACAACAGAGA AGAGTTGACT TCAGATGCTA ACATCAGAGA AGTGGGCCTT 
GAAGGGGCAT TATTGACCTT ACTAGATAAA GAAACAGACC AGAAGTTATG CCATGATATC 
AAAGAGACTC TAAATCATAT GCTTACATCT ATGGCAGTGG ATAAGCTCTC CTTCTGGTTA 
AAGCTTTGTA AAGATGTACT TGCTGCATCA GCTGATTTTA CAACTGTGAC TTGTGTGGAT 
ACAATGCAAG AAGAGGAAGG AGATAAAGGG GATGATGCTT CAGTTCTGAC CACTGGAAAT 
GATGACAAGT CTTTTCCTTT CACCAATCCT CGTTGGGCTA CTAGAGTCTT TGCTGCCGAA 
TGTGTCTGTA GGATAATTAA CCAGTGTGAG AATGCACACA GCGCACATTT TGACATTGCT 
TTAGCACAAG AAATGAAAAA AAGGGATTCA AGAAATGACT TTTTGGTGCT ACATCTTGCC 
GACTTAATTC GCATGGCCTT TATGGCTGCC ACAGATCACA GTGACCAGCT CCGACTTTCT 
GGCCTTGAAA CACTATTAGT TGTTATTCGG CGATTTGCAG CTATTCCAGA ACCAGAGTTT 
CCAGGTCATG TGATTCTGGA ACAGTATCAA GCCAATGTAG GAGCTGCACT TAGGCCAGCC 
TTCACTTCAG AGACGCCACC TGATGTCACT GCCAAAGCAT GTCAGGTTTG CAGTGCCTGG 
ATAGCAAGTG GAGTTGTAAG TGACCTTAAT GATTTACGAA GAGTTCATCA GTTGCTTGTT 
TCATCATTAA CAAAAATACA GGCTGGAAAA GAGGCTCTGA GTCACTTATA TAATGAAAGC 
GCTTCTACCA TGGAGATCTT AGCTGTGCTG AAAGCCTGGG CAGAGGTCTA CATAATTGCT 
GTAGAAAGAC ATAAAAGCCA CAGACAACCT TTGAAGACTA TCCCCTGTTC AGAAGAGAGT 
GTCAGAAATG GGCCACATTC AACAGATGGA CTGCTTGACT TAGTCCACAC AGACCTGAGC 
ACACTGAGTA GGCTCTGGCT TGCTGCACTT CAGGATTTTG CTCTCTTAAC TTTGCCTTCA 
GAATTTGCCT CCCAACTTCC TGTTGAAGGT GGTGCTTTCT ACACAGCAGA GACTAGTGAA 
AATGCAAAAT TACATTATTA CAACTCCTGG GCGCTTATCC TGCATGCTAC AGCATTGTGG 
CTTACCAGCA CAGGTTTTGT TGTTGCTGAT CCGGATGAAG AAGCAGCTCA TCTTTCAAGA 
CCTGTAACAC CAACTTCCAT GTGTCAGGGT TCATCATCTG GAGCTACAGT TAAGTCCCCT 
GAAGATGTCT GCACTGATAG ATTCCATCTT ATTTTAGGAA TAAGTGTGGA GTTTCTGTGT 
TCCTTACGCT CAGATGCAAC CATGGAAAGC ATTACTGCTT GTTTACATGC CTTACAGGCC 
CTTCTAGATG TTCCTTGGCC CAGATCTAAA ATTGGCAGTG ATCAGGATTT GGGCATCGAG 
TTGTTGAATG TACTACATCG AGTAATTTTG ACCCGAGAAT CACCCTCTAT TCAATTGGCT 
TCACTTGAAG TGGTAAGACA GATTATCTGT GCAGCTCAAG AACATGTCAA GGAAAAAAGA 
CGAAGTGCAG AAGTTGATGA TGGGGCTGCC GAAAAGGAAA CCCTGCCAGA GTTTGGAGAG 
GGAAAGGACA CAGGAGGACT TGTGCCTGGG AAATCTTTGG TTTTTGCAAC ACTGGAATTG 
TGTGTATGCA TTTTAGTTAG ACAGCTTCCA GAACTAAATC CTAAATTGAC AGGCAGCCCA 
GGAGTAAAAG CTACAAAGCC ACAGGTGCTG TCTGAGGATG GAAGTAGATT GGTGTCAGCT 
GCGTTAGTTA TCCTCTCTGA ACTTCCTGCC GTGTGCTCTC CTGAAGGAAG CATCTCAATT 
CTCCCTACTA TATTATACCT TACAATTGGG GTCCTCAGAG AAACTGCTGT GAAGTTACCT 
GGAGGCCAGT TACCTTCAAC TGTTGCAGCT TCCCTACAGG CTCTGAAAGG AATATTGTCT 
TCTCCCATGG CCAGAGCAGA AAAGAGTCAC AGTGCTTGGA CTGACCTCCT CCGTAGTGCT 
TTAACAACAA TACTTGACTG TTGGGATCCA GATGGAACAC ATCAAGAACT TGATGAAATC 
AGTCTACTTA CTGCTATCAC AGTATTTATT TTGTCTACCA GTCCAGAAGT TACTACCATC 
CCATGCCTTC AGAAGCGCTG TATTGAGAAA TTTAAGGCTA CTCTTGAGAT AAAAGATCCT 
GTGGTACAAA TCAAGACCTA TCAGCTACTA CTTTCCATTT TTCAGTATCC AAATCCAGCT 
GTTTCTTACC CATACATATA CTCTTTAGTA TCCTCTATTG TGGAAAAGCT ACAGGAAATA 
GACAAGAGAA AGCCTGAAGA CACTGCTGAG CTTCAGATCT TTCAAGAAGG CATAAAGGTC 
TTGCAAGCAC TGGTGGCCAT TGCCGAAGAA CAACACCGTT CTCAGCTGGT GGCCTGTCTC 
CTGCCCATCC TCATTTCCTT CCTTTTGGAT GATAATGATC TGGGATCTGC AACTTCTGTA 
ATGAAAAATT TACATGACTT CGCTTTGCAA AATCTCATGC AGATTGGGCC TCAGTATTCA 
TCAGTTTTTA AAAATGTAAT GGCTTCTTCT CCAGCCCTGA AAGCCCGCCT TGAGGCTGCT 
ATCAAGGGCA ATCAGGAAAG TGTAAAAATC AAGATACCAA CATCTAAACA TACTAAGAAC 
CCTGGAAAGA ATACAAGCAT CCAATTAAAG ACCAACTTCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_856117.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_861210.2 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X9, mRNA.

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CloneID	OCe10253
Clone ID Related Accession (Same CDS sequence)	XM_856117.3 , XM_856117.4
Accession Version	XM_856117.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_856117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGT GAATCTTTGT 
AGTCCCTATT TGTTACTGAG AAGAGCAGTA CTGGCCTGTC TCCGTCAACT TGTACAGAGA 
GAAGCAGCTG AAGTTTCAGA ACATGCTGTT TTGCTTGCTA AAGACAACAG AGAAGAGTTG 
ACTTCAGATG CTAACATCAG AGAAGTGGGC CTTGAAGGGG CATTATTGAC CTTACTAGAT 
AAAGAAACAG ACCAGAAGTT ATGCCATGAT ATCAAAGAGA CTCTAAATCA TATGCTTACA 
TCTATGGCAG TGGATAAGCT CTCCTTCTGG TTAAAGCTTT GTAAAGATGT ACTTGCTGCA 
TCAGCTGATT TTACAACTGT GACTTGTGTG GATACAATGC AAGAAGAGGA AGGAGATAAA 
GGGGATGATG CTTCAGTTCT GACCACTGGA AATGATGACA AGTCTTTTCC TTTCACCAAT 
CCTCGTTGGG CTACTAGAGT CTTTGCTGCC GAATGTGTCT GTAGGATAAT TAACCAGTGT 
GAGAATGCAC ACAGCGCACA TTTTGACATT GCTTTAGCAC AAGAAATGAA AAAAAGGGAT 
TCAAGAAATG ACTTTTTGGT GCTACATCTT GCCGACTTAA TTCGCATGGC CTTTATGGCT 
GCCACAGATC ACAGTGACCA GCTCCGACTT TCTGGCCTTG AAACACTATT AGTTGTTATT 
CGGCGATTTG CAGCTATTCC AGAACCAGAG TTTCCAGGTC ATGTGATTCT GGAACAGTAT 
CAAGCCAATG TAGGAGCTGC ACTTAGGCCA GCCTTCACTT CAGAGACGCC ACCTGATGTC 
ACTGCCAAAG CATGTCAGGT TTGCAGTGCC TGGATAGCAA GTGGAGTTGT AAGTGACCTT 
AATGATTTAC GAAGAGTTCA TCAGTTGCTT GTTTCATCAT TAACAAAAAT ACAGGCTGGA 
AAAGAGGCTC TGAGTCACTT ATATAATGAA AGCGCTTCTA CCATGGAGAT CTTAGCTGTG 
CTGAAAGCCT GGGCAGAGGT CTACATAATT GCTGTAGAAA GACATAAAAG CCACAGACAA 
CCTTTGAAGA CTATCCCCTG TTCAGAAGAG AGTGTCAGAA ATGGGCCACA TTCAACAGAT 
GGACTGCTTG ACTTAGTCCA CACAGACCTG AGCACACTGA GTAGGCTCTG GCTTGCTGCA 
CTTCAGGATT TTGCTCTCTT AACTTTGCCT TCAGAATTTG CCTCCCAACT TCCTGTTGAA 
GGTGGTGCTT TCTACACAGC AGAGACTAGT GAAAATGCAA AATTACATTA TTACAACTCC 
TGGGCGCTTA TCCTGCATGC TACAGCATTG TGGCTTACCA GCACAGGTTT TGTTGTTGCT 
GATCCGGATG AAGAAGCAGC TCATCTTTCA AGACCTGTAA CACCAACTTC CATGTGTCAG 
GGTTCATCAT CTGGAGCTAC AGTTAAGTCC CCTGAAGATG TCTGCACTGA TAGATTCCAT 
CTTATTTTAG GAATAAGTGT GGAGTTTCTG TGTTCCTTAC GCTCAGATGC AACCATGGAA 
AGCATTACTG CTTGTTTACA TGCCTTACAG GCCCTTCTAG ATGTTCCTTG GCCCAGATCT 
AAAATTGGCA GTGATCAGGA TTTGGGCATC GAGTTGTTGA ATGTACTACA TCGAGTAATT 
TTGACCCGAG AATCACCCTC TATTCAATTG GCTTCACTTG AAGTGGTAAG ACAGATTATC 
TGTGCAGCTC AAGAACATGT CAAGGAAAAA AGACGAAGTG CAGAAGTTGA TGATGGGGCT 
GCCGAAAAGG AAACCCTGCC AGAGTTTGGA GAGGGAAAGG ACACAGGAGG ACTTGTGCCT 
GGGAAATCTT TGGTTTTTGC AACACTGGAA TTGTGTGTAT GCATTTTAGT TAGACAGCTT 
CCAGAACTAA ATCCTAAATT GACAGGCAGC CCAGGAGTAA AAGCTACAAA GCCACAGGTG 
CTGTCTGAGG ATGGAAGTAG ATTGGTGTCA GCTGCGTTAG TTATCCTCTC TGAACTTCCT 
GCCGTGTGCT CTCCTGAAGG AAGCATCTCA ATTCTCCCTA CTATATTATA CCTTACAATT 
GGGGTCCTCA GAGAAACTGC TGTGAAGTTA CCTGGAGGCC AGTTACCTTC AACTGTTGCA 
GCTTCCCTAC AGGCTCTGAA AGGAATATTG TCTTCTCCCA TGGCCAGAGC AGAAAAGAGT 
CACAGTGCTT GGACTGACCT CCTCCGTAGT GCTTTAACAA CAATACTTGA CTGTTGGGAT 
CCAGTAGATG GAACACATCA AGAACTTGAT GAAATCAGTC TACTTACTGC TATCACAGTA 
TTTATTTTGT CTACCAGTCC AGAAGTTACT ACCATCCCAT GCCTTCAGAA GCGCTGTATT 
GAGAAATTTA AGGCTACTCT TGAGATAAAA GATCCTGTGG TACAAATCAA GACCTATCAG 
CTACTACTTT CCATTTTTCA GTATCCAAAT CCAGCTGTTT CTTACCCATA CATATACTCT 
TTAGTATCCT CTATTGTGGA AAAGCTACAG GAAATAGACA AGAGAAAGCC TGAAGACACT 
GCTGAGCTTC AGATCTTTCA AGAAGGCATA AAGGTCTTGC AAGCACTGGT GGCCATTGCC 
GAAGAACAAC ACCGTTCTCA GCTGGTGGCC TGTCTCCTGC CCATCCTCAT TTCCTTCCTT 
TTGGATGATA ATGATCTGGG ATCTGCAACT TCTGTAATGA AAAATTTACA TGACTTCGCT 
TTGCAAAATC TCATGCAGAT TGGGCCTCAG TATTCATCAG TTTTTAAAAA TGTAATGGCT 
TCTTCTCCAG CCCTGAAAGC CCGCCTTGAG GCTGCTATCA AGGGCAATCA GGAAAGTGTA 
AAAATCAAGA TACCAACATC TAAACATACT AAGAACCCTG GAAAGAATAC AAGCATCCAA 
TTAAAGACCA ACTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_861210.2
CDS	190..6330
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_856117.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_856117.3

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGT GAATCTTTGT 
AGTCCCTATT TGTTACTGAG AAGAGCAGTA CTGGCCTGTC TCCGTCAACT TGTACAGAGA 
GAAGCAGCTG AAGTTTCAGA ACATGCTGTT TTGCTTGCTA AAGACAACAG AGAAGAGTTG 
ACTTCAGATG CTAACATCAG AGAAGTGGGC CTTGAAGGGG CATTATTGAC CTTACTAGAT 
AAAGAAACAG ACCAGAAGTT ATGCCATGAT ATCAAAGAGA CTCTAAATCA TATGCTTACA 
TCTATGGCAG TGGATAAGCT CTCCTTCTGG TTAAAGCTTT GTAAAGATGT ACTTGCTGCA 
TCAGCTGATT TTACAACTGT GACTTGTGTG GATACAATGC AAGAAGAGGA AGGAGATAAA 
GGGGATGATG CTTCAGTTCT GACCACTGGA AATGATGACA AGTCTTTTCC TTTCACCAAT 
CCTCGTTGGG CTACTAGAGT CTTTGCTGCC GAATGTGTCT GTAGGATAAT TAACCAGTGT 
GAGAATGCAC ACAGCGCACA TTTTGACATT GCTTTAGCAC AAGAAATGAA AAAAAGGGAT 
TCAAGAAATG ACTTTTTGGT GCTACATCTT GCCGACTTAA TTCGCATGGC CTTTATGGCT 
GCCACAGATC ACAGTGACCA GCTCCGACTT TCTGGCCTTG AAACACTATT AGTTGTTATT 
CGGCGATTTG CAGCTATTCC AGAACCAGAG TTTCCAGGTC ATGTGATTCT GGAACAGTAT 
CAAGCCAATG TAGGAGCTGC ACTTAGGCCA GCCTTCACTT CAGAGACGCC ACCTGATGTC 
ACTGCCAAAG CATGTCAGGT TTGCAGTGCC TGGATAGCAA GTGGAGTTGT AAGTGACCTT 
AATGATTTAC GAAGAGTTCA TCAGTTGCTT GTTTCATCAT TAACAAAAAT ACAGGCTGGA 
AAAGAGGCTC TGAGTCACTT ATATAATGAA AGCGCTTCTA CCATGGAGAT CTTAGCTGTG 
CTGAAAGCCT GGGCAGAGGT CTACATAATT GCTGTAGAAA GACATAAAAG CCACAGACAA 
CCTTTGAAGA CTATCCCCTG TTCAGAAGAG AGTGTCAGAA ATGGGCCACA TTCAACAGAT 
GGACTGCTTG ACTTAGTCCA CACAGACCTG AGCACACTGA GTAGGCTCTG GCTTGCTGCA 
CTTCAGGATT TTGCTCTCTT AACTTTGCCT TCAGAATTTG CCTCCCAACT TCCTGTTGAA 
GGTGGTGCTT TCTACACAGC AGAGACTAGT GAAAATGCAA AATTACATTA TTACAACTCC 
TGGGCGCTTA TCCTGCATGC TACAGCATTG TGGCTTACCA GCACAGGTTT TGTTGTTGCT 
GATCCGGATG AAGAAGCAGC TCATCTTTCA AGACCTGTAA CACCAACTTC CATGTGTCAG 
GGTTCATCAT CTGGAGCTAC AGTTAAGTCC CCTGAAGATG TCTGCACTGA TAGATTCCAT 
CTTATTTTAG GAATAAGTGT GGAGTTTCTG TGTTCCTTAC GCTCAGATGC AACCATGGAA 
AGCATTACTG CTTGTTTACA TGCCTTACAG GCCCTTCTAG ATGTTCCTTG GCCCAGATCT 
AAAATTGGCA GTGATCAGGA TTTGGGCATC GAGTTGTTGA ATGTACTACA TCGAGTAATT 
TTGACCCGAG AATCACCCTC TATTCAATTG GCTTCACTTG AAGTGGTAAG ACAGATTATC 
TGTGCAGCTC AAGAACATGT CAAGGAAAAA AGACGAAGTG CAGAAGTTGA TGATGGGGCT 
GCCGAAAAGG AAACCCTGCC AGAGTTTGGA GAGGGAAAGG ACACAGGAGG ACTTGTGCCT 
GGGAAATCTT TGGTTTTTGC AACACTGGAA TTGTGTGTAT GCATTTTAGT TAGACAGCTT 
CCAGAACTAA ATCCTAAATT GACAGGCAGC CCAGGAGTAA AAGCTACAAA GCCACAGGTG 
CTGTCTGAGG ATGGAAGTAG ATTGGTGTCA GCTGCGTTAG TTATCCTCTC TGAACTTCCT 
GCCGTGTGCT CTCCTGAAGG AAGCATCTCA ATTCTCCCTA CTATATTATA CCTTACAATT 
GGGGTCCTCA GAGAAACTGC TGTGAAGTTA CCTGGAGGCC AGTTACCTTC AACTGTTGCA 
GCTTCCCTAC AGGCTCTGAA AGGAATATTG TCTTCTCCCA TGGCCAGAGC AGAAAAGAGT 
CACAGTGCTT GGACTGACCT CCTCCGTAGT GCTTTAACAA CAATACTTGA CTGTTGGGAT 
CCAGTAGATG GAACACATCA AGAACTTGAT GAAATCAGTC TACTTACTGC TATCACAGTA 
TTTATTTTGT CTACCAGTCC AGAAGTTACT ACCATCCCAT GCCTTCAGAA GCGCTGTATT 
GAGAAATTTA AGGCTACTCT TGAGATAAAA GATCCTGTGG TACAAATCAA GACCTATCAG 
CTACTACTTT CCATTTTTCA GTATCCAAAT CCAGCTGTTT CTTACCCATA CATATACTCT 
TTAGTATCCT CTATTGTGGA AAAGCTACAG GAAATAGACA AGAGAAAGCC TGAAGACACT 
GCTGAGCTTC AGATCTTTCA AGAAGGCATA AAGGTCTTGC AAGCACTGGT GGCCATTGCC 
GAAGAACAAC ACCGTTCTCA GCTGGTGGCC TGTCTCCTGC CCATCCTCAT TTCCTTCCTT 
TTGGATGATA ATGATCTGGG ATCTGCAACT TCTGTAATGA AAAATTTACA TGACTTCGCT 
TTGCAAAATC TCATGCAGAT TGGGCCTCAG TATTCATCAG TTTTTAAAAA TGTAATGGCT 
TCTTCTCCAG CCCTGAAAGC CCGCCTTGAG GCTGCTATCA AGGGCAATCA GGAAAGTGTA 
AAAATCAAGA TACCAACATC TAAACATACT AAGAACCCTG GAAAGAATAC AAGCATCCAA 
TTAAAGACCA ACTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_856117.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_861210.2 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X11, mRNA.

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CloneID	OCe10253
Clone ID Related Accession (Same CDS sequence)	XM_856117.3 , XM_856117.4
Accession Version	XM_856117.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6141bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	HEAT repeat-containing protein 5A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_856117.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGT GAATCTTTGT 
AGTCCCTATT TGTTACTGAG AAGAGCAGTA CTGGCCTGTC TCCGTCAACT TGTACAGAGA 
GAAGCAGCTG AAGTTTCAGA ACATGCTGTT TTGCTTGCTA AAGACAACAG AGAAGAGTTG 
ACTTCAGATG CTAACATCAG AGAAGTGGGC CTTGAAGGGG CATTATTGAC CTTACTAGAT 
AAAGAAACAG ACCAGAAGTT ATGCCATGAT ATCAAAGAGA CTCTAAATCA TATGCTTACA 
TCTATGGCAG TGGATAAGCT CTCCTTCTGG TTAAAGCTTT GTAAAGATGT ACTTGCTGCA 
TCAGCTGATT TTACAACTGT GACTTGTGTG GATACAATGC AAGAAGAGGA AGGAGATAAA 
GGGGATGATG CTTCAGTTCT GACCACTGGA AATGATGACA AGTCTTTTCC TTTCACCAAT 
CCTCGTTGGG CTACTAGAGT CTTTGCTGCC GAATGTGTCT GTAGGATAAT TAACCAGTGT 
GAGAATGCAC ACAGCGCACA TTTTGACATT GCTTTAGCAC AAGAAATGAA AAAAAGGGAT 
TCAAGAAATG ACTTTTTGGT GCTACATCTT GCCGACTTAA TTCGCATGGC CTTTATGGCT 
GCCACAGATC ACAGTGACCA GCTCCGACTT TCTGGCCTTG AAACACTATT AGTTGTTATT 
CGGCGATTTG CAGCTATTCC AGAACCAGAG TTTCCAGGTC ATGTGATTCT GGAACAGTAT 
CAAGCCAATG TAGGAGCTGC ACTTAGGCCA GCCTTCACTT CAGAGACGCC ACCTGATGTC 
ACTGCCAAAG CATGTCAGGT TTGCAGTGCC TGGATAGCAA GTGGAGTTGT AAGTGACCTT 
AATGATTTAC GAAGAGTTCA TCAGTTGCTT GTTTCATCAT TAACAAAAAT ACAGGCTGGA 
AAAGAGGCTC TGAGTCACTT ATATAATGAA AGCGCTTCTA CCATGGAGAT CTTAGCTGTG 
CTGAAAGCCT GGGCAGAGGT CTACATAATT GCTGTAGAAA GACATAAAAG CCACAGACAA 
CCTTTGAAGA CTATCCCCTG TTCAGAAGAG AGTGTCAGAA ATGGGCCACA TTCAACAGAT 
GGACTGCTTG ACTTAGTCCA CACAGACCTG AGCACACTGA GTAGGCTCTG GCTTGCTGCA 
CTTCAGGATT TTGCTCTCTT AACTTTGCCT TCAGAATTTG CCTCCCAACT TCCTGTTGAA 
GGTGGTGCTT TCTACACAGC AGAGACTAGT GAAAATGCAA AATTACATTA TTACAACTCC 
TGGGCGCTTA TCCTGCATGC TACAGCATTG TGGCTTACCA GCACAGGTTT TGTTGTTGCT 
GATCCGGATG AAGAAGCAGC TCATCTTTCA AGACCTGTAA CACCAACTTC CATGTGTCAG 
GGTTCATCAT CTGGAGCTAC AGTTAAGTCC CCTGAAGATG TCTGCACTGA TAGATTCCAT 
CTTATTTTAG GAATAAGTGT GGAGTTTCTG TGTTCCTTAC GCTCAGATGC AACCATGGAA 
AGCATTACTG CTTGTTTACA TGCCTTACAG GCCCTTCTAG ATGTTCCTTG GCCCAGATCT 
AAAATTGGCA GTGATCAGGA TTTGGGCATC GAGTTGTTGA ATGTACTACA TCGAGTAATT 
TTGACCCGAG AATCACCCTC TATTCAATTG GCTTCACTTG AAGTGGTAAG ACAGATTATC 
TGTGCAGCTC AAGAACATGT CAAGGAAAAA AGACGAAGTG CAGAAGTTGA TGATGGGGCT 
GCCGAAAAGG AAACCCTGCC AGAGTTTGGA GAGGGAAAGG ACACAGGAGG ACTTGTGCCT 
GGGAAATCTT TGGTTTTTGC AACACTGGAA TTGTGTGTAT GCATTTTAGT TAGACAGCTT 
CCAGAACTAA ATCCTAAATT GACAGGCAGC CCAGGAGTAA AAGCTACAAA GCCACAGGTG 
CTGTCTGAGG ATGGAAGTAG ATTGGTGTCA GCTGCGTTAG TTATCCTCTC TGAACTTCCT 
GCCGTGTGCT CTCCTGAAGG AAGCATCTCA ATTCTCCCTA CTATATTATA CCTTACAATT 
GGGGTCCTCA GAGAAACTGC TGTGAAGTTA CCTGGAGGCC AGTTACCTTC AACTGTTGCA 
GCTTCCCTAC AGGCTCTGAA AGGAATATTG TCTTCTCCCA TGGCCAGAGC AGAAAAGAGT 
CACAGTGCTT GGACTGACCT CCTCCGTAGT GCTTTAACAA CAATACTTGA CTGTTGGGAT 
CCAGTAGATG GAACACATCA AGAACTTGAT GAAATCAGTC TACTTACTGC TATCACAGTA 
TTTATTTTGT CTACCAGTCC AGAAGTTACT ACCATCCCAT GCCTTCAGAA GCGCTGTATT 
GAGAAATTTA AGGCTACTCT TGAGATAAAA GATCCTGTGG TACAAATCAA GACCTATCAG 
CTACTACTTT CCATTTTTCA GTATCCAAAT CCAGCTGTTT CTTACCCATA CATATACTCT 
TTAGTATCCT CTATTGTGGA AAAGCTACAG GAAATAGACA AGAGAAAGCC TGAAGACACT 
GCTGAGCTTC AGATCTTTCA AGAAGGCATA AAGGTCTTGC AAGCACTGGT GGCCATTGCC 
GAAGAACAAC ACCGTTCTCA GCTGGTGGCC TGTCTCCTGC CCATCCTCAT TTCCTTCCTT 
TTGGATGATA ATGATCTGGG ATCTGCAACT TCTGTAATGA AAAATTTACA TGACTTCGCT 
TTGCAAAATC TCATGCAGAT TGGGCCTCAG TATTCATCAG TTTTTAAAAA TGTAATGGCT 
TCTTCTCCAG CCCTGAAAGC CCGCCTTGAG GCTGCTATCA AGGGCAATCA GGAAAGTGTA 
AAAATCAAGA TACCAACATC TAAACATACT AAGAACCCTG GAAAGAATAC AAGCATCCAA 
TTAAAGACCA ACTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_861210.2
CDS	207..6347
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDAVMNDGNMEIRLGSTDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_856117.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_856117.4

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGCTGTGATG 
AATGACGGTA ATATGGAAAT CCGGCTTGGC TCCACAGATG TGACAGCCAG CCAGCATATG 
CTGGTGTGTG CTTTACAAGA ACTTGGAAAT CTCATATACA GTCTTGGCAC CACAGCTGCA 
CCTTTACTGC AGGATTCAAG TGCAGGCATT CTTGATAGTG TCATTTCAGT TATTCTACAT 
CCTAGCATTT CTGTTCGACT AGCAACGGCT TGGTGTTTAC ACTGCATTGC TGTGGCATTA 
CCTTCTTACC TAACACCTCT TTTAGACCGG TGCCTGGAAC GGCTTACTGT ACTTAAGTCT 
TCACCGGAAG CAGTGACTGG CTTTAGTTTT GCTGTGGCAG CTTTGTTGGG AGCAGTGAAA 
CATTGTCCTT TAGGAATTCC TCATGGGAAA GGCAAGATTA TTATGACTTT AGCAGAAGAT 
TTACTGTGTT CAGCTGTACA AAACAGTCGT CTTTCAGCTC AGCGCACACA AGCTGGTTGG 
TTGTTGATTG CTGCTCTGAT GACATTAGGT CCTGCAGTTG TCAGTCATCA CCTTGCTCGA 
CTTCTGCTAT TGTGGAAGTG TGTCTTTCCA GTGTCTCCTA AAGATCTAGA AACAGAAAAG 
AGCCGAGGAG ATTCATTTAC CTGGCAGGTA ACCTTGGAAG GACGAGCTGG TGCGCTCTGT 
GCTGTCAAGA GTTTTGTTTC CCACTGTGGT GACCTTCTTA CTGAGGAAGT AATTCAACGT 
CTTCTTCCAC CACTTCCTTG TGCTGTGGAT TTGCTAACTC AGCTTTCTTC AGTACTAAAA 
ACATACGGAA GCACTTTGAA AATGCCATCA GTAGTTTATA GACAAAGGCT TTATGAATTA 
CTGATTTTAT TACCTCCTGA AACCTATGAA GAAAACTTCT GTGCTATCCT CAAAGAGCTG 
GCTGCTGACT TGACTGCCTC TGATATACAG GTGGCAGCAT CTGCATTTTT ACTTCCACCC 
CTCTGTCATC AGGATGATCT TGTGATATTA AGTCCTTTGC TACAAGAAAC TGATCATAGA 
TTTATTGAAG AACAGCTCCT GCTTGGTAAT GGTATTGCTT GTGGAAGTTT GGAGTATGAC 
CCTTATTCTA TTTATGAGAA AGATGTAGAG GGAGATTCAG TGCCTAAACC CCTGTCTCCA 
GTACTTTCAG TTATTAGCTC TGCAGTCAAG CTCTTTGGAG TTATATGTGC TCATGTGGGA 
GAAGCTCAAA GGCTTTCTAT ACTGGAACAG CTTTTGGACA GTATAAAGCA CACAAAAGGA 
GCTCGTCAAC AAGTAGTTCA GTTACATGTT GTTTCTTCAC TCTCTAGTTT CTTGAAGTAC 
TTAGCTGGCT CCAAGGGAAA CTTGGGTTCA GAAGAAATGA GAAAACCTGC CCTAGCATTG 
GTGATGGGAG CCCTAGAAAG TCCCAACCCC CTCTTGAGAT GTGCAGCTGC AGAGGCATGG 
GCTAGATTAG CCCAAGTGGT TAATGATGGA GCTTTTACTG CTGCATTAGC TCAAGTTAGC 
TTTGACAAAC TGAAATCAGC AAGGGATGTG GTTACCAGAA CAGGATACTC ATTGGCTTTG 
GGATCTCTAC ATAGATATTT AGGAGGAATA AGTTCTTCTC AACATTTGAG TTCTTGTGTT 
GGAATCCTTT ATACTTTGTC TCAGGACAGT ACTTCTCCTG ATGTGCAGAC CTGGGCACTA 
CATTCGCTAT CATTGATAAT TGATTCTGCT GGCCCCCTCT ATCATATGCA TGTGGAACCC 
ACCCTTTCTC TTATTATAAT GTTACTGTTA AATGTGCCTC CTACTCATGC TGAAGTACAC 
CAGAGTCTTG GTCGCTGTTT GAATGCCCTC ATTACTACAT TGGGTCCAGA GCTACAGGGT 
AATAGTACTT CCATTTCTAC CTTAAGGACT TCCTGTCTTT TGGGTTGTGC AGTGATGCAA 
GATAACCCAG ACTGCCTCGT TCAAGCTCAG GCCATCTCTT GCCTTCAGCA GCTTCATATG 
TTTGCACCAA GACATGTCAA CTTGTCCAGC TTGGTCAGCT GCCTCTGTGT GAATCTTTGT 
AGTCCCTATT TGTTACTGAG AAGAGCAGTA CTGGCCTGTC TCCGTCAACT TGTACAGAGA 
GAAGCAGCTG AAGTTTCAGA ACATGCTGTT TTGCTTGCTA AAGACAACAG AGAAGAGTTG 
ACTTCAGATG CTAACATCAG AGAAGTGGGC CTTGAAGGGG CATTATTGAC CTTACTAGAT 
AAAGAAACAG ACCAGAAGTT ATGCCATGAT ATCAAAGAGA CTCTAAATCA TATGCTTACA 
TCTATGGCAG TGGATAAGCT CTCCTTCTGG TTAAAGCTTT GTAAAGATGT ACTTGCTGCA 
TCAGCTGATT TTACAACTGT GACTTGTGTG GATACAATGC AAGAAGAGGA AGGAGATAAA 
GGGGATGATG CTTCAGTTCT GACCACTGGA AATGATGACA AGTCTTTTCC TTTCACCAAT 
CCTCGTTGGG CTACTAGAGT CTTTGCTGCC GAATGTGTCT GTAGGATAAT TAACCAGTGT 
GAGAATGCAC ACAGCGCACA TTTTGACATT GCTTTAGCAC AAGAAATGAA AAAAAGGGAT 
TCAAGAAATG ACTTTTTGGT GCTACATCTT GCCGACTTAA TTCGCATGGC CTTTATGGCT 
GCCACAGATC ACAGTGACCA GCTCCGACTT TCTGGCCTTG AAACACTATT AGTTGTTATT 
CGGCGATTTG CAGCTATTCC AGAACCAGAG TTTCCAGGTC ATGTGATTCT GGAACAGTAT 
CAAGCCAATG TAGGAGCTGC ACTTAGGCCA GCCTTCACTT CAGAGACGCC ACCTGATGTC 
ACTGCCAAAG CATGTCAGGT TTGCAGTGCC TGGATAGCAA GTGGAGTTGT AAGTGACCTT 
AATGATTTAC GAAGAGTTCA TCAGTTGCTT GTTTCATCAT TAACAAAAAT ACAGGCTGGA 
AAAGAGGCTC TGAGTCACTT ATATAATGAA AGCGCTTCTA CCATGGAGAT CTTAGCTGTG 
CTGAAAGCCT GGGCAGAGGT CTACATAATT GCTGTAGAAA GACATAAAAG CCACAGACAA 
CCTTTGAAGA CTATCCCCTG TTCAGAAGAG AGTGTCAGAA ATGGGCCACA TTCAACAGAT 
GGACTGCTTG ACTTAGTCCA CACAGACCTG AGCACACTGA GTAGGCTCTG GCTTGCTGCA 
CTTCAGGATT TTGCTCTCTT AACTTTGCCT TCAGAATTTG CCTCCCAACT TCCTGTTGAA 
GGTGGTGCTT TCTACACAGC AGAGACTAGT GAAAATGCAA AATTACATTA TTACAACTCC 
TGGGCGCTTA TCCTGCATGC TACAGCATTG TGGCTTACCA GCACAGGTTT TGTTGTTGCT 
GATCCGGATG AAGAAGCAGC TCATCTTTCA AGACCTGTAA CACCAACTTC CATGTGTCAG 
GGTTCATCAT CTGGAGCTAC AGTTAAGTCC CCTGAAGATG TCTGCACTGA TAGATTCCAT 
CTTATTTTAG GAATAAGTGT GGAGTTTCTG TGTTCCTTAC GCTCAGATGC AACCATGGAA 
AGCATTACTG CTTGTTTACA TGCCTTACAG GCCCTTCTAG ATGTTCCTTG GCCCAGATCT 
AAAATTGGCA GTGATCAGGA TTTGGGCATC GAGTTGTTGA ATGTACTACA TCGAGTAATT 
TTGACCCGAG AATCACCCTC TATTCAATTG GCTTCACTTG AAGTGGTAAG ACAGATTATC 
TGTGCAGCTC AAGAACATGT CAAGGAAAAA AGACGAAGTG CAGAAGTTGA TGATGGGGCT 
GCCGAAAAGG AAACCCTGCC AGAGTTTGGA GAGGGAAAGG ACACAGGAGG ACTTGTGCCT 
GGGAAATCTT TGGTTTTTGC AACACTGGAA TTGTGTGTAT GCATTTTAGT TAGACAGCTT 
CCAGAACTAA ATCCTAAATT GACAGGCAGC CCAGGAGTAA AAGCTACAAA GCCACAGGTG 
CTGTCTGAGG ATGGAAGTAG ATTGGTGTCA GCTGCGTTAG TTATCCTCTC TGAACTTCCT 
GCCGTGTGCT CTCCTGAAGG AAGCATCTCA ATTCTCCCTA CTATATTATA CCTTACAATT 
GGGGTCCTCA GAGAAACTGC TGTGAAGTTA CCTGGAGGCC AGTTACCTTC AACTGTTGCA 
GCTTCCCTAC AGGCTCTGAA AGGAATATTG TCTTCTCCCA TGGCCAGAGC AGAAAAGAGT 
CACAGTGCTT GGACTGACCT CCTCCGTAGT GCTTTAACAA CAATACTTGA CTGTTGGGAT 
CCAGTAGATG GAACACATCA AGAACTTGAT GAAATCAGTC TACTTACTGC TATCACAGTA 
TTTATTTTGT CTACCAGTCC AGAAGTTACT ACCATCCCAT GCCTTCAGAA GCGCTGTATT 
GAGAAATTTA AGGCTACTCT TGAGATAAAA GATCCTGTGG TACAAATCAA GACCTATCAG 
CTACTACTTT CCATTTTTCA GTATCCAAAT CCAGCTGTTT CTTACCCATA CATATACTCT 
TTAGTATCCT CTATTGTGGA AAAGCTACAG GAAATAGACA AGAGAAAGCC TGAAGACACT 
GCTGAGCTTC AGATCTTTCA AGAAGGCATA AAGGTCTTGC AAGCACTGGT GGCCATTGCC 
GAAGAACAAC ACCGTTCTCA GCTGGTGGCC TGTCTCCTGC CCATCCTCAT TTCCTTCCTT 
TTGGATGATA ATGATCTGGG ATCTGCAACT TCTGTAATGA AAAATTTACA TGACTTCGCT 
TTGCAAAATC TCATGCAGAT TGGGCCTCAG TATTCATCAG TTTTTAAAAA TGTAATGGCT 
TCTTCTCCAG CCCTGAAAGC CCGCCTTGAG GCTGCTATCA AGGGCAATCA GGAAAGTGTA 
AAAATCAAGA TACCAACATC TAAACATACT AAGAACCCTG GAAAGAATAC AAGCATCCAA 
TTAAAGACCA ACTTCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEATR5A ( XM_005623304.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005623361.1 Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X10, mRNA.

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CloneID	OCe10256
Clone ID Related Accession (Same CDS sequence)	XM_005623304.1
Accession Version	XM_005623304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	HEAT repeat-containing protein 5A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGTGACAGCC 
AGCCAGCATA TGCTGGTGTG TGCTTTACAA GAACTTGGAA ATCTCATATA CAGTCTTGGC 
ACCACAGCTG CACCTTTACT GCAGGATTCA AGTGCAGGCA TTCTTGATAG TGTCATTTCA 
GTTATTCTAC ATCCTAGCAT TTCTGTTCGA CTAGCAACGG CTTGGTGTTT ACACTGCATT 
GCTGTGGCAT TACCTTCTTA CCTAACACCT CTTTTAGACC GGTGCCTGGA ACGGCTTACT 
GTACTTAAGT CTTCACCGGA AGCAGTGACT GGCTTTAGTT TTGCTGTGGC AGCTTTGTTG 
GGAGCAGTGA AACATTGTCC TTTAGGAATT CCTCATGGGA AAGGCAAGAT TATTATGACT 
TTAGCAGAAG ATTTACTGTG TTCAGCTGTA CAAAACAGTC GTCTTTCAGC TCAGCGCACA 
CAAGCTGGTT GGTTGTTGAT TGCTGCTCTG ATGACATTAG GTCCTGCAGT TGTCAGTCAT 
CACCTTGCTC GACTTCTGCT ATTGTGGAAG TGTGTCTTTC CAGTGTCTCC TAAAGATCTA 
GAAACAGAAA AGAGCCGAGG AGATTCATTT ACCTGGCAGG TAACCTTGGA AGGACGAGCT 
GGTGCGCTCT GTGCTGTCAA GAGTTTTGTT TCCCACTGTG GTGACCTTCT TACTGAGGAA 
GTAATTCAAC GTCTTCTTCC ACCACTTCCT TGTGCTGTGG ATTTGCTAAC TCAGCTTTCT 
TCAGTACTAA AAACATACGG AAGCACTTTG AAAATGCCAT CAGTAGTTTA TAGACAAAGG 
CTTTATGAAT TACTGATTTT ATTACCTCCT GAAACCTATG AAGAAAACTT CTGTGCTATC 
CTCAAAGAGC TGGCTGCTGA CTTGACTGCC TCTGATATAC AGGTGGCAGC ATCTGCATTT 
TTACTTCCAC CCCTCTGTCA TCAGGATGAT CTTGTGATAT TAAGTCCTTT GCTACAAGAA 
ACTGATCATA GATTTATTGA AGAACAGCTC CTGCTTGGTA ATGGTATTGC TTGTGGAAGT 
TTGGAGTATG ACCCTTATTC TATTTATGAG AAAGATGTAG AGGGAGATTC AGTGCCTAAA 
CCCCTGTCTC CAGTACTTTC AGTTATTAGC TCTGCAGTCA AGCTCTTTGG AGTTATATGT 
GCTCATGTGG GAGAAGCTCA AAGGCTTTCT ATACTGGAAC AGCTTTTGGA CAGTATAAAG 
CACACAAAAG GAGCTCGTCA ACAAGTAGTT CAGTTACATG TTGTTTCTTC ACTCTCTAGT 
TTCTTGAAGT ACTTAGCTGG CTCCAAGGGA AACTTGGGTT CAGAAGAAAT GAGAAAACCT 
GCCCTAGCAT TGGTGATGGG AGCCCTAGAA AGTCCCAACC CCCTCTTGAG ATGTGCAGCT 
GCAGAGGCAT GGGCTAGATT AGCCCAAGTG GTTAATGATG GAGCTTTTAC TGCTGCATTA 
GCTCAAGTTA GCTTTGACAA ACTGAAATCA GCAAGGGATG TGGTTACCAG AACAGGATAC 
TCATTGGCTT TGGGATCTCT ACATAGATAT TTAGGAGGAA TAAGTTCTTC TCAACATTTG 
AGTTCTTGTG TTGGAATCCT TTATACTTTG TCTCAGGACA GTACTTCTCC TGATGTGCAG 
ACCTGGGCAC TACATTCGCT ATCATTGATA ATTGATTCTG CTGGCCCCCT CTATCATATG 
CATGTGGAAC CCACCCTTTC TCTTATTATA ATGTTACTGT TAAATGTGCC TCCTACTCAT 
GCTGAAGTAC ACCAGAGTCT TGGTCGCTGT TTGAATGCCC TCATTACTAC ATTGGGTCCA 
GAGCTACAGG GTAATAGTAC TTCCATTTCT ACCTTAAGGA CTTCCTGTCT TTTGGGTTGT 
GCAGTGATGC AAGATAACCC AGACTGCCTC GTTCAAGCTC AGGCCATCTC TTGCCTTCAG 
CAGCTTCATA TGTTTGCACC AAGACATGTC AACTTGTCCA GCTTGGTCAG CTGCCTCTGT 
GAGATCTTGT TGGATAATTC TGTGTTGGTG AATCTTTGTA GTCCCTATTT GTTACTGAGA 
AGAGCAGTAC TGGCCTGTCT CCGTCAACTT GTACAGAGAG AAGCAGCTGA AGTTTCAGAA 
CATGCTGTTT TGCTTGCTAA AGACAACAGA GAAGAGTTGA CTTCAGATGC TAACATCAGA 
GAAGTGGGCC TTGAAGGGGC ATTATTGACC TTACTAGATA AAGAAACAGA CCAGAAGTTA 
TGCCATGATA TCAAAGAGAC TCTAAATCAT ATGCTTACAT CTATGGCAGT GGATAAGCTC 
TCCTTCTGGT TAAAGCTTTG TAAAGATGTA CTTGCTGCAT CAGCTGATTT TACAACTGTG 
ACTTGTGTGG ATACAATGCA AGAAGAGGAA GGAGATAAAG GGGATGATGC TTCAGTTCTG 
ACCACTGGAA ATGATGACAA GTCTTTTCCT TTCACCAATC CTCGTTGGGC TACTAGAGTC 
TTTGCTGCCG AATGTGTCTG TAGGATAATT AACCAGTGTG AGAATGCACA CAGCGCACAT 
TTTGACATTG CTTTAGCACA AGAAATGAAA AAAAGGGATT CAAGAAATGA CTTTTTGGTG 
CTACATCTTG CCGACTTAAT TCGCATGGCC TTTATGGCTG CCACAGATCA CAGTGACCAG 
CTCCGACTTT CTGGCCTTGA AACACTATTA GTTGTTATTC GGCGATTTGC AGCTATTCCA 
GAACCAGAGT TTCCAGGTCA TGTGATTCTG GAACAGTATC AAGCCAATGT AGGAGCTGCA 
CTTAGGCCAG CCTTCACTTC AGAGACGCCA CCTGATGTCA CTGCCAAAGC ATGTCAGGTT 
TGCAGTGCCT GGATAGCAAG TGGAGTTGTA AGTGACCTTA ATGATTTACG AAGAGTTCAT 
CAGTTGCTTG TTTCATCATT AACAAAAATA CAGGCTGGAA AAGAGGCTCT GAGTCACTTA 
TATAATGAAA GCGCTTCTAC CATGGAGATC TTAGCTGTGC TGAAAGCCTG GGCAGAGGTC 
TACATAATTG CTGTAGAAAG ACATAAAAGC CACAGACAAC CTTTGAAGAC TATCCCCTGT 
TCAGAAGAGA GTGTCAGAAA TGGGCCACAT TCAACAGATG GACTGCTTGA CTTAGTCCAC 
ACAGACCTGA GCACACTGAG TAGGCTCTGG CTTGCTGCAC TTCAGGATTT TGCTCTCTTA 
ACTTTGCCTT CAGAATTTGC CTCCCAACTT CCTGTTGAAG GTGGTGCTTT CTACACAGCA 
GAGACTAGTG AAAATGCAAA ATTACATTAT TACAACTCCT GGGCGCTTAT CCTGCATGCT 
ACAGCATTGT GGCTTACCAG CACAGGTTTT GTTGTTGCTG ATCCGGATGA AGAAGCAGCT 
CATCTTTCAA GACCTGTAAC ACCAACTTCC ATGTGTCAGG GTTCATCATC TGGAGCTACA 
GTTAAGTCCC CTGAAGATGT CTGCACTGAT AGATTCCATC TTATTTTAGG AATAAGTGTG 
GAGTTTCTGT GTTCCTTACG CTCAGATGCA ACCATGGAAA GCATTACTGC TTGTTTACAT 
GCCTTACAGG CCCTTCTAGA TGTTCCTTGG CCCAGATCTA AAATTGGCAG TGATCAGGAT 
TTGGGCATCG AGTTGTTGAA TGTACTACAT CGAGTAATTT TGACCCGAGA ATCACCCTCT 
ATTCAATTGG CTTCACTTGA AGTGGTAAGA CAGATTATCT GTGCAGCTCA AGAACATGTC 
AAGGAAAAAA GACGAAGTGC AGAAGTTGAT GATGGGGCTG CCGAAAAGGA AACCCTGCCA 
GAGTTTGGAG AGGGAAAGGA CACAGGAGGA CTTGTGCCTG GGAAATCTTT GGTTTTTGCA 
ACACTGGAAT TGTGTGTATG CATTTTAGTT AGACAGCTTC CAGAACTAAA TCCTAAATTG 
ACAGGCAGCC CAGGAGTAAA AGCTACAAAG CCACAGGTGC TGTCTGAGGA TGGAAGTAGA 
TTGGTGTCAG CTGCGTTAGT TATCCTCTCT GAACTTCCTG CCGTGTGCTC TCCTGAAGGA 
AGCATCTCAA TTCTCCCTAC TATATTATAC CTTACAATTG GGGTCCTCAG AGAAACTGCT 
GTGAAGTTAC CTGGAGGCCA GTTACCTTCA ACTGTTGCAG CTTCCCTACA GGCTCTGAAA 
GGAATATTGT CTTCTCCCAT GGCCAGAGCA GAAAAGAGTC ACAGTGCTTG GACTGACCTC 
CTCCGTAGTG CTTTAACAAC AATACTTGAC TGTTGGGATC CAGTAGATGG AACACATCAA 
GAACTTGATG AAATCAGTCT ACTTACTGCT ATCACAGTAT TTATTTTGTC TACCAGTCCA 
GAAGTTACTA CCATCCCATG CCTTCAGAAG CGCTGTATTG AGAAATTTAA GGCTACTCTT 
GAGATAAAAG ATCCTGTGGT ACAAATCAAG ACCTATCAGC TACTACTTTC CATTTTTCAG 
TATCCAAATC CAGCTGTTTC TTACCCATAC ATATACTCTT TAGTATCCTC TATTGTGGAA 
AAGCTACAGG AAATAGACAA GAGAAAGCCT GAAGACACTG CTGAGCTTCA GATCTTTCAA 
GAAGGCATAA AGGTCTTGCA AGCACTGGTG GCCATTGCCG AAGAACAACA CCGTTCTCAG 
CTGGTGGCCT GTCTCCTGCC CATCCTCATT TCCTTCCTTT TGGATGATAA TGATCTGGGA 
TCTGCAACTT CTGTAATGAA AAATTTACAT GACTTCGCTT TGCAAAATCT CATGCAGATT 
GGGCCTCAGT ATTCATCAGT TTTTAAAAAT GTAATGGCTT CTTCTCCAGC CCTGAAAGCC 
CGCCTTGAGG CTGCTATCAA GGGCAATCAG GAAAGTGTAA AAATCAAGAT ACCAACATCT 
AAACATACTA AGAACCCTGG AAAGAATACA AGCATCCAAT TAAAGACCAA CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623361.1
CDS	190..6309
Translation	MELAHSLLLNEEAYNQLGEVQKAEFIFDWLRYLEKLLLATSKSDVKEKQKALVEQLLSLLNSSPGPPTRKLLAKNLGVLYSIGDTFSVYETIDKCNDLIRSKDDSPSYLPTKLAAVVCLGSLYKKLGRILVNTFTDTVGNILKAMKSAESQGRYEIMLSLQNILNGLGAAATPCHRDVYKAAKSCLTDRSMAVRCAAAKCLLELQKEAIFMWSTDLDSVATLCFKSFEGSNYDVRISVSKLLGTVLARAIISKHPGAAVSRQSIRRVSLEEVLELLGTGFLRGSSGFLRASGDMLKGTSSVSRDVRVGVTQAYVVFVSTLGGTWLEKNFATFLSHILSLVSQSHPKATQTQTDAVCGRRCVSFILRATIGGLLGEKAQIAAAKDICQAIWKLKKVVDVTASQHMLVCALQELGNLIYSLGTTAAPLLQDSSAGILDSVISVILHPSISVRLATAWCLHCIAVALPSYLTPLLDRCLERLTVLKSSPEAVTGFSFAVAALLGAVKHCPLGIPHGKGKIIMTLAEDLLCSAVQNSRLSAQRTQAGWLLIAALMTLGPAVVSHHLARLLLLWKCVFPVSPKDLETEKSRGDSFTWQVTLEGRAGALCAVKSFVSHCGDLLTEEVIQRLLPPLPCAVDLLTQLSSVLKTYGSTLKMPSVVYRQRLYELLILLPPETYEENFCAILKELAADLTASDIQVAASAFLLPPLCHQDDLVILSPLLQETDHRFIEEQLLLGNGIACGSLEYDPYSIYEKDVEGDSVPKPLSPVLSVISSAVKLFGVICAHVGEAQRLSILEQLLDSIKHTKGARQQVVQLHVVSSLSSFLKYLAGSKGNLGSEEMRKPALALVMGALESPNPLLRCAAAEAWARLAQVVNDGAFTAALAQVSFDKLKSARDVVTRTGYSLALGSLHRYLGGISSSQHLSSCVGILYTLSQDSTSPDVQTWALHSLSLIIDSAGPLYHMHVEPTLSLIIMLLLNVPPTHAEVHQSLGRCLNALITTLGPELQGNSTSISTLRTSCLLGCAVMQDNPDCLVQAQAISCLQQLHMFAPRHVNLSSLVSCLCEILLDNSVLVNLCSPYLLLRRAVLACLRQLVQREAAEVSEHAVLLAKDNREELTSDANIREVGLEGALLTLLDKETDQKLCHDIKETLNHMLTSMAVDKLSFWLKLCKDVLAASADFTTVTCVDTMQEEEGDKGDDASVLTTGNDDKSFPFTNPRWATRVFAAECVCRIINQCENAHSAHFDIALAQEMKKRDSRNDFLVLHLADLIRMAFMAATDHSDQLRLSGLETLLVVIRRFAAIPEPEFPGHVILEQYQANVGAALRPAFTSETPPDVTAKACQVCSAWIASGVVSDLNDLRRVHQLLVSSLTKIQAGKEALSHLYNESASTMEILAVLKAWAEVYIIAVERHKSHRQPLKTIPCSEESVRNGPHSTDGLLDLVHTDLSTLSRLWLAALQDFALLTLPSEFASQLPVEGGAFYTAETSENAKLHYYNSWALILHATALWLTSTGFVVADPDEEAAHLSRPVTPTSMCQGSSSGATVKSPEDVCTDRFHLILGISVEFLCSLRSDATMESITACLHALQALLDVPWPRSKIGSDQDLGIELLNVLHRVILTRESPSIQLASLEVVRQIICAAQEHVKEKRRSAEVDDGAAEKETLPEFGEGKDTGGLVPGKSLVFATLELCVCILVRQLPELNPKLTGSPGVKATKPQVLSEDGSRLVSAALVILSELPAVCSPEGSISILPTILYLTIGVLRETAVKLPGGQLPSTVAASLQALKGILSSPMARAEKSHSAWTDLLRSALTTILDCWDPVDGTHQELDEISLLTAITVFILSTSPEVTTIPCLQKRCIEKFKATLEIKDPVVQIKTYQLLLSIFQYPNPAVSYPYIYSLVSSIVEKLQEIDKRKPEDTAELQIFQEGIKVLQALVAIAEEQHRSQLVACLLPILISFLLDDNDLGSATSVMKNLHDFALQNLMQIGPQYSSVFKNVMASSPALKARLEAAIKGNQESVKIKIPTSKHTKNPGKNTSIQLKTNFL

Target ORF information:

RefSeq Version	XM_005623304.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HEAT repeat containing 5A (HEATR5A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623304.1

ORF Insert Sequence:

ATGGAATTAG CTCATAGCTT ACTGCTGAAT GAAGAAGCAT ACAATCAACT AGGTGAAGTT 
CAGAAGGCAG AGTTTATTTT CGATTGGTTG AGATATTTGG AGAAGCTCTT GCTAGCAACG 
AGCAAGAGTG ATGTAAAGGA GAAACAGAAG GCTCTTGTTG AACAGCTCCT GTCTTTGTTG 
AACAGCTCCC CAGGGCCTCC TACCCGCAAA CTCCTTGCTA AGAATCTAGG TGTTCTTTAT 
AGTATCGGGG ACACATTCTC TGTTTACGAG ACAATCGATA AATGTAATGA TCTTATTCGT 
AGCAAGGATG ATTCTCCAAG TTATCTTCCC ACAAAACTGG CTGCCGTGGT ATGTTTGGGT 
TCCTTATACA AGAAGTTGGG TAGAATACTG GTTAACACCT TTACTGATAC AGTGGGGAAT 
ATCCTTAAAG CTATGAAGAG TGCAGAGTCT CAAGGGCGAT ATGAGATTAT GCTGAGCCTG 
CAAAATATAT TGAATGGACT AGGAGCTGCT GCTACACCTT GCCACAGGGA TGTTTATAAA 
GCTGCTAAAT CCTGCTTAAC AGATAGATCC ATGGCTGTTC GTTGTGCTGC TGCAAAGTGT 
CTCCTTGAAC TTCAAAAAGA GGCCATCTTT ATGTGGAGTA CAGACCTGGA TAGTGTGGCC 
ACACTGTGTT TTAAGTCCTT TGAAGGTTCC AATTATGATG TGCGGATTTC AGTTTCAAAA 
CTACTAGGCA CAGTGTTGGC TAGAGCTATA ATTTCTAAAC ATCCAGGAGC AGCAGTCTCA 
CGTCAAAGCA TTCGCAGAGT ATCTCTGGAG GAAGTTCTGG AATTACTAGG AACAGGGTTT 
CTACGTGGAA GTTCAGGATT TCTTCGAGCC AGTGGAGATA TGCTGAAAGG AACCAGTTCA 
GTCAGTAGGG ATGTTCGAGT TGGAGTTACT CAGGCTTATG TGGTATTTGT TTCAACATTA 
GGAGGAACAT GGCTAGAGAA AAATTTTGCT ACCTTTCTTT CTCATATCCT AAGCCTTGTG 
TCACAGTCGC ACCCCAAAGC CACCCAAACT CAGACTGATG CTGTCTGCGG TCGCCGCTGT 
GTTTCATTTA TCCTTCGAGC TACTATAGGA GGTCTTCTTG GAGAAAAGGC CCAAATTGCT 
GCTGCCAAGG ATATTTGCCA GGCTATCTGG AAGTTAAAGA AAGTTGTGGA TGTGACAGCC 
AGCCAGCATA TGCTGGTGTG TGCTTTACAA GAACTTGGAA ATCTCATATA CAGTCTTGGC 
ACCACAGCTG CACCTTTACT GCAGGATTCA AGTGCAGGCA TTCTTGATAG TGTCATTTCA 
GTTATTCTAC ATCCTAGCAT TTCTGTTCGA CTAGCAACGG CTTGGTGTTT ACACTGCATT 
GCTGTGGCAT TACCTTCTTA CCTAACACCT CTTTTAGACC GGTGCCTGGA ACGGCTTACT 
GTACTTAAGT CTTCACCGGA AGCAGTGACT GGCTTTAGTT TTGCTGTGGC AGCTTTGTTG 
GGAGCAGTGA AACATTGTCC TTTAGGAATT CCTCATGGGA AAGGCAAGAT TATTATGACT 
TTAGCAGAAG ATTTACTGTG TTCAGCTGTA CAAAACAGTC GTCTTTCAGC TCAGCGCACA 
CAAGCTGGTT GGTTGTTGAT TGCTGCTCTG ATGACATTAG GTCCTGCAGT TGTCAGTCAT 
CACCTTGCTC GACTTCTGCT ATTGTGGAAG TGTGTCTTTC CAGTGTCTCC TAAAGATCTA 
GAAACAGAAA AGAGCCGAGG AGATTCATTT ACCTGGCAGG TAACCTTGGA AGGACGAGCT 
GGTGCGCTCT GTGCTGTCAA GAGTTTTGTT TCCCACTGTG GTGACCTTCT TACTGAGGAA 
GTAATTCAAC GTCTTCTTCC ACCACTTCCT TGTGCTGTGG ATTTGCTAAC TCAGCTTTCT 
TCAGTACTAA AAACATACGG AAGCACTTTG AAAATGCCAT CAGTAGTTTA TAGACAAAGG 
CTTTATGAAT TACTGATTTT ATTACCTCCT GAAACCTATG AAGAAAACTT CTGTGCTATC 
CTCAAAGAGC TGGCTGCTGA CTTGACTGCC TCTGATATAC AGGTGGCAGC ATCTGCATTT 
TTACTTCCAC CCCTCTGTCA TCAGGATGAT CTTGTGATAT TAAGTCCTTT GCTACAAGAA 
ACTGATCATA GATTTATTGA AGAACAGCTC CTGCTTGGTA ATGGTATTGC TTGTGGAAGT 
TTGGAGTATG ACCCTTATTC TATTTATGAG AAAGATGTAG AGGGAGATTC AGTGCCTAAA 
CCCCTGTCTC CAGTACTTTC AGTTATTAGC TCTGCAGTCA AGCTCTTTGG AGTTATATGT 
GCTCATGTGG GAGAAGCTCA AAGGCTTTCT ATACTGGAAC AGCTTTTGGA CAGTATAAAG 
CACACAAAAG GAGCTCGTCA ACAAGTAGTT CAGTTACATG TTGTTTCTTC ACTCTCTAGT 
TTCTTGAAGT ACTTAGCTGG CTCCAAGGGA AACTTGGGTT CAGAAGAAAT GAGAAAACCT 
GCCCTAGCAT TGGTGATGGG AGCCCTAGAA AGTCCCAACC CCCTCTTGAG ATGTGCAGCT 
GCAGAGGCAT GGGCTAGATT AGCCCAAGTG GTTAATGATG GAGCTTTTAC TGCTGCATTA 
GCTCAAGTTA GCTTTGACAA ACTGAAATCA GCAAGGGATG TGGTTACCAG AACAGGATAC 
TCATTGGCTT TGGGATCTCT ACATAGATAT TTAGGAGGAA TAAGTTCTTC TCAACATTTG 
AGTTCTTGTG TTGGAATCCT TTATACTTTG TCTCAGGACA GTACTTCTCC TGATGTGCAG 
ACCTGGGCAC TACATTCGCT ATCATTGATA ATTGATTCTG CTGGCCCCCT CTATCATATG 
CATGTGGAAC CCACCCTTTC TCTTATTATA ATGTTACTGT TAAATGTGCC TCCTACTCAT 
GCTGAAGTAC ACCAGAGTCT TGGTCGCTGT TTGAATGCCC TCATTACTAC ATTGGGTCCA 
GAGCTACAGG GTAATAGTAC TTCCATTTCT ACCTTAAGGA CTTCCTGTCT TTTGGGTTGT 
GCAGTGATGC AAGATAACCC AGACTGCCTC GTTCAAGCTC AGGCCATCTC TTGCCTTCAG 
CAGCTTCATA TGTTTGCACC AAGACATGTC AACTTGTCCA GCTTGGTCAG CTGCCTCTGT 
GAGATCTTGT TGGATAATTC TGTGTTGGTG AATCTTTGTA GTCCCTATTT GTTACTGAGA 
AGAGCAGTAC TGGCCTGTCT CCGTCAACTT GTACAGAGAG AAGCAGCTGA AGTTTCAGAA 
CATGCTGTTT TGCTTGCTAA AGACAACAGA GAAGAGTTGA CTTCAGATGC TAACATCAGA 
GAAGTGGGCC TTGAAGGGGC ATTATTGACC TTACTAGATA AAGAAACAGA CCAGAAGTTA 
TGCCATGATA TCAAAGAGAC TCTAAATCAT ATGCTTACAT CTATGGCAGT GGATAAGCTC 
TCCTTCTGGT TAAAGCTTTG TAAAGATGTA CTTGCTGCAT CAGCTGATTT TACAACTGTG 
ACTTGTGTGG ATACAATGCA AGAAGAGGAA GGAGATAAAG GGGATGATGC TTCAGTTCTG 
ACCACTGGAA ATGATGACAA GTCTTTTCCT TTCACCAATC CTCGTTGGGC TACTAGAGTC 
TTTGCTGCCG AATGTGTCTG TAGGATAATT AACCAGTGTG AGAATGCACA CAGCGCACAT 
TTTGACATTG CTTTAGCACA AGAAATGAAA AAAAGGGATT CAAGAAATGA CTTTTTGGTG 
CTACATCTTG CCGACTTAAT TCGCATGGCC TTTATGGCTG CCACAGATCA CAGTGACCAG 
CTCCGACTTT CTGGCCTTGA AACACTATTA GTTGTTATTC GGCGATTTGC AGCTATTCCA 
GAACCAGAGT TTCCAGGTCA TGTGATTCTG GAACAGTATC AAGCCAATGT AGGAGCTGCA 
CTTAGGCCAG CCTTCACTTC AGAGACGCCA CCTGATGTCA CTGCCAAAGC ATGTCAGGTT 
TGCAGTGCCT GGATAGCAAG TGGAGTTGTA AGTGACCTTA ATGATTTACG AAGAGTTCAT 
CAGTTGCTTG TTTCATCATT AACAAAAATA CAGGCTGGAA AAGAGGCTCT GAGTCACTTA 
TATAATGAAA GCGCTTCTAC CATGGAGATC TTAGCTGTGC TGAAAGCCTG GGCAGAGGTC 
TACATAATTG CTGTAGAAAG ACATAAAAGC CACAGACAAC CTTTGAAGAC TATCCCCTGT 
TCAGAAGAGA GTGTCAGAAA TGGGCCACAT TCAACAGATG GACTGCTTGA CTTAGTCCAC 
ACAGACCTGA GCACACTGAG TAGGCTCTGG CTTGCTGCAC TTCAGGATTT TGCTCTCTTA 
ACTTTGCCTT CAGAATTTGC CTCCCAACTT CCTGTTGAAG GTGGTGCTTT CTACACAGCA 
GAGACTAGTG AAAATGCAAA ATTACATTAT TACAACTCCT GGGCGCTTAT CCTGCATGCT 
ACAGCATTGT GGCTTACCAG CACAGGTTTT GTTGTTGCTG ATCCGGATGA AGAAGCAGCT 
CATCTTTCAA GACCTGTAAC ACCAACTTCC ATGTGTCAGG GTTCATCATC TGGAGCTACA 
GTTAAGTCCC CTGAAGATGT CTGCACTGAT AGATTCCATC TTATTTTAGG AATAAGTGTG 
GAGTTTCTGT GTTCCTTACG CTCAGATGCA ACCATGGAAA GCATTACTGC TTGTTTACAT 
GCCTTACAGG CCCTTCTAGA TGTTCCTTGG CCCAGATCTA AAATTGGCAG TGATCAGGAT 
TTGGGCATCG AGTTGTTGAA TGTACTACAT CGAGTAATTT TGACCCGAGA ATCACCCTCT 
ATTCAATTGG CTTCACTTGA AGTGGTAAGA CAGATTATCT GTGCAGCTCA AGAACATGTC 
AAGGAAAAAA GACGAAGTGC AGAAGTTGAT GATGGGGCTG CCGAAAAGGA AACCCTGCCA 
GAGTTTGGAG AGGGAAAGGA CACAGGAGGA CTTGTGCCTG GGAAATCTTT GGTTTTTGCA 
ACACTGGAAT TGTGTGTATG CATTTTAGTT AGACAGCTTC CAGAACTAAA TCCTAAATTG 
ACAGGCAGCC CAGGAGTAAA AGCTACAAAG CCACAGGTGC TGTCTGAGGA TGGAAGTAGA 
TTGGTGTCAG CTGCGTTAGT TATCCTCTCT GAACTTCCTG CCGTGTGCTC TCCTGAAGGA 
AGCATCTCAA TTCTCCCTAC TATATTATAC CTTACAATTG GGGTCCTCAG AGAAACTGCT 
GTGAAGTTAC CTGGAGGCCA GTTACCTTCA ACTGTTGCAG CTTCCCTACA GGCTCTGAAA 
GGAATATTGT CTTCTCCCAT GGCCAGAGCA GAAAAGAGTC ACAGTGCTTG GACTGACCTC 
CTCCGTAGTG CTTTAACAAC AATACTTGAC TGTTGGGATC CAGTAGATGG AACACATCAA 
GAACTTGATG AAATCAGTCT ACTTACTGCT ATCACAGTAT TTATTTTGTC TACCAGTCCA 
GAAGTTACTA CCATCCCATG CCTTCAGAAG CGCTGTATTG AGAAATTTAA GGCTACTCTT 
GAGATAAAAG ATCCTGTGGT ACAAATCAAG ACCTATCAGC TACTACTTTC CATTTTTCAG 
TATCCAAATC CAGCTGTTTC TTACCCATAC ATATACTCTT TAGTATCCTC TATTGTGGAA 
AAGCTACAGG AAATAGACAA GAGAAAGCCT GAAGACACTG CTGAGCTTCA GATCTTTCAA 
GAAGGCATAA AGGTCTTGCA AGCACTGGTG GCCATTGCCG AAGAACAACA CCGTTCTCAG 
CTGGTGGCCT GTCTCCTGCC CATCCTCATT TCCTTCCTTT TGGATGATAA TGATCTGGGA 
TCTGCAACTT CTGTAATGAA AAATTTACAT GACTTCGCTT TGCAAAATCT CATGCAGATT 
GGGCCTCAGT ATTCATCAGT TTTTAAAAAT GTAATGGCTT CTTCTCCAGC CCTGAAAGCC 
CGCCTTGAGG CTGCTATCAA GGGCAATCAG GAAAGTGTAA AAATCAAGAT ACCAACATCT 
AAACATACTA AGAACCCTGG AAAGAATACA AGCATCCAAT TAAAGACCAA CTTCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HEATR5A
Entrez Gene ID	480285
Full Name	HEAT repeat containing 5A
General protein information	Preferred Names HEAT repeat containing 5A Names HEAT repeat-containing protein 5A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Bos taurus (cattle)	HEATR5A	XP_005222124.1
Mus musculus (house mouse)	Heatr5a	NP_796145.2
Gallus gallus (chicken)	HEATR5A	XP_004941794.1
Homo sapiens (human)	HEATR5A	NP_056288.2
Danio rerio (zebrafish)	heatr5a	NP_956772.2
Canis lupus familiaris (dog)	HEATR5A	XP_005623357.1
Pan troglodytes (chimpanzee)	HEATR5A	XP_522993.3
	LOC716984	XP_001108090.2
Rattus norvegicus (Norway rat)	Heatr5a	XP_006240152.1
Xenopus tropicalis (tropical clawed frog)	heatr5a	NP_001090667.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HEATR5A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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