RNF31 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF31
Entrez Gene ID	480259
Full Name	ring finger protein 31
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005623257.3	XP_005623314.1	E3 ubiquitin-protein ligase RNF31 isoform X4
XM_005623255.3	XP_005623312.1	E3 ubiquitin-protein ligase RNF31 isoform X1
XM_005623256.3	XP_005623313.1	E3 ubiquitin-protein ligase RNF31 isoform X2
XM_537383.6	XP_537383.2	E3 ubiquitin-protein ligase RNF31 isoform X3
XM_005623256.2	XP_005623313.1	E3 ubiquitin-protein ligase RNF31 isoform X2
XM_005623255.2	XP_005623312.1	E3 ubiquitin-protein ligase RNF31 isoform X1
XM_537383.5	XP_537383.2	E3 ubiquitin-protein ligase RNF31 isoform X3
XM_005623257.2	XP_005623314.1	E3 ubiquitin-protein ligase RNF31 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	RNF31	NP_060469.4
Pan troglodytes (chimpanzee)	RNF31	XP_001166671.1
Canis lupus familiaris (dog)	RNF31	XP_537383.2
Xenopus tropicalis (tropical clawed frog)	rnf31	NP_001090644.1
	RNF31	XP_001112195.1
Mus musculus (house mouse)	Rnf31	NP_919327.2
Rattus norvegicus (Norway rat)	Rnf31	NP_001102338.2
Danio rerio (zebrafish)	LOC100334103	XP_002660873.3

Gene Ontology

The following RNF31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe10192	XM_005623257.3 Latest version!	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe10189	XM_005623255.3 Latest version!	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe10190	XM_005623256.3 Latest version!	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe10191	XM_537383.6 Latest version!	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe10190	XM_005623256.2	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe10189	XM_005623255.2	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe10191	XM_537383.5	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe10192	XM_005623257.2	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF31 ( XM_005623257.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623314.1 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe10192
Clone ID Related Accession (Same CDS sequence)	XM_005623257.3 , XM_005623257.2
Accession Version	XM_005623257.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623257.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATGG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623314.1
CDS	261..3017
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDVRKILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNGF

Target ORF information:

RefSeq Version	XM_005623257.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623257.3

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATGG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_005623257.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623314.1 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe10192
Clone ID Related Accession (Same CDS sequence)	XM_005623257.3 , XM_005623257.2
Accession Version	XM_005623257.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623257.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATGG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623314.1
CDS	265..3021
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDVRKILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNGF

Target ORF information:

RefSeq Version	XM_005623257.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623257.2

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATGG GTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_005623255.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623312.1 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe10189
Clone ID Related Accession (Same CDS sequence)	XM_005623255.3 , XM_005623255.2
Accession Version	XM_005623255.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623255.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATAA ATGTCCAGAC 
CCTAACTGCA GGGTGAAAAA GTCCCTGCAT GGCCACCACC CACGAGACTG CCTCTTTTAC 
CTGCGGGACT GGACTGCTCT CCGGCTCCAG AAGCTGCTAC AGGACAATAA TGTCATGTTC 
AACACAGAAC CCCCAGCAGG GGCCCGGGCA GTTCCTGGCG GTGGCTGCCG AGTGATGGAG 
CAGAAGGAAG TCCCTAATGG GTTCCGGGAT GAAGCTTGTG GCAAGGAGAC TCCAGCTGGC 
CATGCTGGCC TCTGCCAGGC ACACTACAAA GAGTATCTTG TGAGCCTCAT CAACGCGCAC 
TCACTGGACC CAGCCACCCT ATATGAGGTG GAGGAGCTGG AGACGGCTGC TGAGCGCTAC 
CTGCACGTGC GCCCTCAGCC AGTGGCAGGG GAGGATGCCC CTGCCTACCA TGCCCGCTTG 
TTACAGAAGC TGATGGAAGA GGTGCCCTTG GGACAGAGTA TCCCTCGCAG GAGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623312.1
CDS	257..3496
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDVRKILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVMEQKEVPNGFRDEACGKETPAGHAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETAAERYLHVRPQPVAGEDAPAYHARLLQKLMEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	XM_005623255.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623255.3

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATAA ATGTCCAGAC 
CCTAACTGCA GGGTGAAAAA GTCCCTGCAT GGCCACCACC CACGAGACTG CCTCTTTTAC 
CTGCGGGACT GGACTGCTCT CCGGCTCCAG AAGCTGCTAC AGGACAATAA TGTCATGTTC 
AACACAGAAC CCCCAGCAGG GGCCCGGGCA GTTCCTGGCG GTGGCTGCCG AGTGATGGAG 
CAGAAGGAAG TCCCTAATGG GTTCCGGGAT GAAGCTTGTG GCAAGGAGAC TCCAGCTGGC 
CATGCTGGCC TCTGCCAGGC ACACTACAAA GAGTATCTTG TGAGCCTCAT CAACGCGCAC 
TCACTGGACC CAGCCACCCT ATATGAGGTG GAGGAGCTGG AGACGGCTGC TGAGCGCTAC 
CTGCACGTGC GCCCTCAGCC AGTGGCAGGG GAGGATGCCC CTGCCTACCA TGCCCGCTTG 
TTACAGAAGC TGATGGAAGA GGTGCCCTTG GGACAGAGTA TCCCTCGCAG GAGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_005623255.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623312.1 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe10189
Clone ID Related Accession (Same CDS sequence)	XM_005623255.3 , XM_005623255.2
Accession Version	XM_005623255.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATAA ATGTCCAGAC 
CCTAACTGCA GGGTGAAAAA GTCCCTGCAT GGCCACCACC CACGAGACTG CCTCTTTTAC 
CTGCGGGACT GGACTGCTCT CCGGCTCCAG AAGCTGCTAC AGGACAATAA TGTCATGTTC 
AACACAGAAC CCCCAGCAGG GGCCCGGGCA GTTCCTGGCG GTGGCTGCCG AGTGATGGAG 
CAGAAGGAAG TCCCTAATGG GTTCCGGGAT GAAGCTTGTG GCAAGGAGAC TCCAGCTGGC 
CATGCTGGCC TCTGCCAGGC ACACTACAAA GAGTATCTTG TGAGCCTCAT CAACGCGCAC 
TCACTGGACC CAGCCACCCT ATATGAGGTG GAGGAGCTGG AGACGGCTGC TGAGCGCTAC 
CTGCACGTGC GCCCTCAGCC AGTGGCAGGG GAGGATGCCC CTGCCTACCA TGCCCGCTTG 
TTACAGAAGC TGATGGAAGA GGTGCCCTTG GGACAGAGTA TCCCTCGCAG GAGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623312.1
CDS	265..3504
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDVRKILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVMEQKEVPNGFRDEACGKETPAGHAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETAAERYLHVRPQPVAGEDAPAYHARLLQKLMEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	XM_005623255.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623255.2

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGCA GGGGGATACT TTGCTCTCCT CTGCCCAGCC TCCAGTCTGG 
TACTGTATTC ACTGTACCTT CTGCAACTCG GGCCCTGGCT GGGTGTGTGC TATGTGCAAC 
CGGACCAGCA GCCCCATCCT GGTACAGCAG GCCCCCCAGC TCCATGCCAG CTCTTTGGAA 
GAGCGACTTC CTGAGCCACG GCCTCCACGG TGCCTCAGTG CCCCCCTGCC CGGTTACTGT 
GGGGACCCTG AGAAGCAGCG CCAGGACAAG ATGCGGGAGG AAGGTCTCCA ACTAGTGATG 
AAGATCCGGG AAGGGGAAGC TGCAGGTGCC TGTCCAGAGG AGGTCTTCTC AGCTCTACAG 
TACTCAGGCA CTGAGGTGCC CCTGCAATGG TTGCGCTCGG AGCTGCCCTA CGTGCTGGAA 
ATGGTGGCCG AGCTGGCAGG ACAGCAGGAC CCGGGGCTGG GTGCCTTTTC CTGTCAGGAG 
GCCCGGAAAG CCTGGCTGGA CCGTCATGGC AATCTTGATG AAGCTGTGGA AGAGTGTGTG 
AGGGCCCGGC GGAGGAAGGT GCAGGAACTC CGGTCCCTGG GCTTTGGGCC CGAGGAGGGG 
TCTCTTCAAG CATTGTTCCA ACATGGTGGT GATGTGGCAC GGGCCCTGAC AGAACTACAG 
CGCCAGCGCC TAGAGCCCTT CCATCAGCGC CTCTGGGACA AGGGCCTTGA TCCCACTCCT 
TCCTGGGATG GGCCAGATAA GCAGAGTCTG GTCAGACGGC TTTTGGCAGT CTACGCCCTC 
CCCAGCTGGG GCCGAGCAGA GCTGGCACTG TCACTGCTGC AGGAGACACC CAGGAATTAT 
GAGCTGGGAG ATGTGGTGGA AGCTGTGAGG CACAGCCAGG ACCGGGCCTT CCTGCGCCGC 
TTGCTTGCCC AGGAGTGTGC CGTGTGTGGC TGGGCCCTGC CCCGCAACCG GATGCAGGCC 
CTGACTTCCT GTGAGTGCAC CATCTGTCCC GACTGCTTCC GCCAGCACTT CACCATCGCC 
CTGAAGGAGA AACACATCAC AGACATGGTG TGCCCCGCCT GTGGCCGCCC TGACCTCACC 
GATGACACGC AGTTGCTCAG CTACTTTTCT ACACTTGACA TCCAGCTTCG GGAGAGCCTG 
GAGCCAGATG CCTATGCACT GTTTCACAAG AAGCTGACTG AGGGTGTGCT CATGCGGGAC 
CCCAAATTCT TGTGGTGTGC CCAATGCTCC TTTGGCTTCA TATATGAACG GGAGCAGCTG 
GAGGCAACAT GTCCCCAGTG TCACCAGACC TTCTGTGTGC GCTGTAAGCG CCAGTGGGAG 
GAGCAGCACC GAGGCCGGAG CTGTGAGGAT TTCCAGAACT GGAAACGCAA CAATGACCCC 
GAATACCAGG CCCAGGGCCT AGCCCTGTAT CTTCAGGAGA ATGGCATCGA CTGCCCCAAG 
TGCAAGTTCT CGTATGCACT GGCCCGAGGA GGCTGCATGC ACTTTCACTG CACCCAATGC 
CGCCACCAGT TCTGCAGCGG CTGCTACAAT GCCTTTTACG CCAAGAATAA ATGTCCAGAC 
CCTAACTGCA GGGTGAAAAA GTCCCTGCAT GGCCACCACC CACGAGACTG CCTCTTTTAC 
CTGCGGGACT GGACTGCTCT CCGGCTCCAG AAGCTGCTAC AGGACAATAA TGTCATGTTC 
AACACAGAAC CCCCAGCAGG GGCCCGGGCA GTTCCTGGCG GTGGCTGCCG AGTGATGGAG 
CAGAAGGAAG TCCCTAATGG GTTCCGGGAT GAAGCTTGTG GCAAGGAGAC TCCAGCTGGC 
CATGCTGGCC TCTGCCAGGC ACACTACAAA GAGTATCTTG TGAGCCTCAT CAACGCGCAC 
TCACTGGACC CAGCCACCCT ATATGAGGTG GAGGAGCTGG AGACGGCTGC TGAGCGCTAC 
CTGCACGTGC GCCCTCAGCC AGTGGCAGGG GAGGATGCCC CTGCCTACCA TGCCCGCTTG 
TTACAGAAGC TGATGGAAGA GGTGCCCTTG GGACAGAGTA TCCCTCGCAG GAGGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_005623256.3 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623313.1 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe10190
Clone ID Related Accession (Same CDS sequence)	XM_005623256.3 , XM_005623256.2
Accession Version	XM_005623256.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005623256.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGGG GGATACTTTG CTCTCCTCTG CCCAGCCTCC AGTCTGGTAC 
TGTATTCACT GTACCTTCTG CAACTCGGGC CCTGGCTGGG TGTGTGCTAT GTGCAACCGG 
ACCAGCAGCC CCATCCTGGT ACAGCAGGCC CCCCAGCTCC ATGCCAGCTC TTTGGAAGAG 
CGACTTCCTG AGCCACGGCC TCCACGGTGC CTCAGTGCCC CCCTGCCCGG TTACTGTGGG 
GACCCTGAGA AGCAGCGCCA GGACAAGATG CGGGAGGAAG GTCTCCAACT AGTGATGAAG 
ATCCGGGAAG GGGAAGCTGC AGGTGCCTGT CCAGAGGAGG TCTTCTCAGC TCTACAGTAC 
TCAGGCACTG AGGTGCCCCT GCAATGGTTG CGCTCGGAGC TGCCCTACGT GCTGGAAATG 
GTGGCCGAGC TGGCAGGACA GCAGGACCCG GGGCTGGGTG CCTTTTCCTG TCAGGAGGCC 
CGGAAAGCCT GGCTGGACCG TCATGGCAAT CTTGATGAAG CTGTGGAAGA GTGTGTGAGG 
GCCCGGCGGA GGAAGGTGCA GGAACTCCGG TCCCTGGGCT TTGGGCCCGA GGAGGGGTCT 
CTTCAAGCAT TGTTCCAACA TGGTGGTGAT GTGGCACGGG CCCTGACAGA ACTACAGCGC 
CAGCGCCTAG AGCCCTTCCA TCAGCGCCTC TGGGACAAGG GCCTTGATCC CACTCCTTCC 
TGGGATGGGC CAGATAAGCA GAGTCTGGTC AGACGGCTTT TGGCAGTCTA CGCCCTCCCC 
AGCTGGGGCC GAGCAGAGCT GGCACTGTCA CTGCTGCAGG AGACACCCAG GAATTATGAG 
CTGGGAGATG TGGTGGAAGC TGTGAGGCAC AGCCAGGACC GGGCCTTCCT GCGCCGCTTG 
CTTGCCCAGG AGTGTGCCGT GTGTGGCTGG GCCCTGCCCC GCAACCGGAT GCAGGCCCTG 
ACTTCCTGTG AGTGCACCAT CTGTCCCGAC TGCTTCCGCC AGCACTTCAC CATCGCCCTG 
AAGGAGAAAC ACATCACAGA CATGGTGTGC CCCGCCTGTG GCCGCCCTGA CCTCACCGAT 
GACACGCAGT TGCTCAGCTA CTTTTCTACA CTTGACATCC AGCTTCGGGA GAGCCTGGAG 
CCAGATGCCT ATGCACTGTT TCACAAGAAG CTGACTGAGG GTGTGCTCAT GCGGGACCCC 
AAATTCTTGT GGTGTGCCCA ATGCTCCTTT GGCTTCATAT ATGAACGGGA GCAGCTGGAG 
GCAACATGTC CCCAGTGTCA CCAGACCTTC TGTGTGCGCT GTAAGCGCCA GTGGGAGGAG 
CAGCACCGAG GCCGGAGCTG TGAGGATTTC CAGAACTGGA AACGCAACAA TGACCCCGAA 
TACCAGGCCC AGGGCCTAGC CCTGTATCTT CAGGAGAATG GCATCGACTG CCCCAAGTGC 
AAGTTCTCGT ATGCACTGGC CCGAGGAGGC TGCATGCACT TTCACTGCAC CCAATGCCGC 
CACCAGTTCT GCAGCGGCTG CTACAATGCC TTTTACGCCA AGAATAAATG TCCAGACCCT 
AACTGCAGGG TGAAAAAGTC CCTGCATGGC CACCACCCAC GAGACTGCCT CTTTTACCTG 
CGGGACTGGA CTGCTCTCCG GCTCCAGAAG CTGCTACAGG ACAATAATGT CATGTTCAAC 
ACAGAACCCC CAGCAGGGGC CCGGGCAGTT CCTGGCGGTG GCTGCCGAGT GATGGAGCAG 
AAGGAAGTCC CTAATGGGTT CCGGGATGAA GCTTGTGGCA AGGAGACTCC AGCTGGCCAT 
GCTGGCCTCT GCCAGGCACA CTACAAAGAG TATCTTGTGA GCCTCATCAA CGCGCACTCA 
CTGGACCCAG CCACCCTATA TGAGGTGGAG GAGCTGGAGA CGGCTGCTGA GCGCTACCTG 
CACGTGCGCC CTCAGCCAGT GGCAGGGGAG GATGCCCCTG CCTACCATGC CCGCTTGTTA 
CAGAAGCTGA TGGAAGAGGT GCCCTTGGGA CAGAGTATCC CTCGCAGGAG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623313.1
CDS	259..3495
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDVRKILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVMEQKEVPNGFRDEACGKETPAGHAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETAAERYLHVRPQPVAGEDAPAYHARLLQKLMEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	XM_005623256.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623256.3

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGGG GGATACTTTG CTCTCCTCTG CCCAGCCTCC AGTCTGGTAC 
TGTATTCACT GTACCTTCTG CAACTCGGGC CCTGGCTGGG TGTGTGCTAT GTGCAACCGG 
ACCAGCAGCC CCATCCTGGT ACAGCAGGCC CCCCAGCTCC ATGCCAGCTC TTTGGAAGAG 
CGACTTCCTG AGCCACGGCC TCCACGGTGC CTCAGTGCCC CCCTGCCCGG TTACTGTGGG 
GACCCTGAGA AGCAGCGCCA GGACAAGATG CGGGAGGAAG GTCTCCAACT AGTGATGAAG 
ATCCGGGAAG GGGAAGCTGC AGGTGCCTGT CCAGAGGAGG TCTTCTCAGC TCTACAGTAC 
TCAGGCACTG AGGTGCCCCT GCAATGGTTG CGCTCGGAGC TGCCCTACGT GCTGGAAATG 
GTGGCCGAGC TGGCAGGACA GCAGGACCCG GGGCTGGGTG CCTTTTCCTG TCAGGAGGCC 
CGGAAAGCCT GGCTGGACCG TCATGGCAAT CTTGATGAAG CTGTGGAAGA GTGTGTGAGG 
GCCCGGCGGA GGAAGGTGCA GGAACTCCGG TCCCTGGGCT TTGGGCCCGA GGAGGGGTCT 
CTTCAAGCAT TGTTCCAACA TGGTGGTGAT GTGGCACGGG CCCTGACAGA ACTACAGCGC 
CAGCGCCTAG AGCCCTTCCA TCAGCGCCTC TGGGACAAGG GCCTTGATCC CACTCCTTCC 
TGGGATGGGC CAGATAAGCA GAGTCTGGTC AGACGGCTTT TGGCAGTCTA CGCCCTCCCC 
AGCTGGGGCC GAGCAGAGCT GGCACTGTCA CTGCTGCAGG AGACACCCAG GAATTATGAG 
CTGGGAGATG TGGTGGAAGC TGTGAGGCAC AGCCAGGACC GGGCCTTCCT GCGCCGCTTG 
CTTGCCCAGG AGTGTGCCGT GTGTGGCTGG GCCCTGCCCC GCAACCGGAT GCAGGCCCTG 
ACTTCCTGTG AGTGCACCAT CTGTCCCGAC TGCTTCCGCC AGCACTTCAC CATCGCCCTG 
AAGGAGAAAC ACATCACAGA CATGGTGTGC CCCGCCTGTG GCCGCCCTGA CCTCACCGAT 
GACACGCAGT TGCTCAGCTA CTTTTCTACA CTTGACATCC AGCTTCGGGA GAGCCTGGAG 
CCAGATGCCT ATGCACTGTT TCACAAGAAG CTGACTGAGG GTGTGCTCAT GCGGGACCCC 
AAATTCTTGT GGTGTGCCCA ATGCTCCTTT GGCTTCATAT ATGAACGGGA GCAGCTGGAG 
GCAACATGTC CCCAGTGTCA CCAGACCTTC TGTGTGCGCT GTAAGCGCCA GTGGGAGGAG 
CAGCACCGAG GCCGGAGCTG TGAGGATTTC CAGAACTGGA AACGCAACAA TGACCCCGAA 
TACCAGGCCC AGGGCCTAGC CCTGTATCTT CAGGAGAATG GCATCGACTG CCCCAAGTGC 
AAGTTCTCGT ATGCACTGGC CCGAGGAGGC TGCATGCACT TTCACTGCAC CCAATGCCGC 
CACCAGTTCT GCAGCGGCTG CTACAATGCC TTTTACGCCA AGAATAAATG TCCAGACCCT 
AACTGCAGGG TGAAAAAGTC CCTGCATGGC CACCACCCAC GAGACTGCCT CTTTTACCTG 
CGGGACTGGA CTGCTCTCCG GCTCCAGAAG CTGCTACAGG ACAATAATGT CATGTTCAAC 
ACAGAACCCC CAGCAGGGGC CCGGGCAGTT CCTGGCGGTG GCTGCCGAGT GATGGAGCAG 
AAGGAAGTCC CTAATGGGTT CCGGGATGAA GCTTGTGGCA AGGAGACTCC AGCTGGCCAT 
GCTGGCCTCT GCCAGGCACA CTACAAAGAG TATCTTGTGA GCCTCATCAA CGCGCACTCA 
CTGGACCCAG CCACCCTATA TGAGGTGGAG GAGCTGGAGA CGGCTGCTGA GCGCTACCTG 
CACGTGCGCC CTCAGCCAGT GGCAGGGGAG GATGCCCCTG CCTACCATGC CCGCTTGTTA 
CAGAAGCTGA TGGAAGAGGT GCCCTTGGGA CAGAGTATCC CTCGCAGGAG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_005623256.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005623313.1 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe10190
Clone ID Related Accession (Same CDS sequence)	XM_005623256.3 , XM_005623256.2
Accession Version	XM_005623256.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005623256.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGGG GGATACTTTG CTCTCCTCTG CCCAGCCTCC AGTCTGGTAC 
TGTATTCACT GTACCTTCTG CAACTCGGGC CCTGGCTGGG TGTGTGCTAT GTGCAACCGG 
ACCAGCAGCC CCATCCTGGT ACAGCAGGCC CCCCAGCTCC ATGCCAGCTC TTTGGAAGAG 
CGACTTCCTG AGCCACGGCC TCCACGGTGC CTCAGTGCCC CCCTGCCCGG TTACTGTGGG 
GACCCTGAGA AGCAGCGCCA GGACAAGATG CGGGAGGAAG GTCTCCAACT AGTGATGAAG 
ATCCGGGAAG GGGAAGCTGC AGGTGCCTGT CCAGAGGAGG TCTTCTCAGC TCTACAGTAC 
TCAGGCACTG AGGTGCCCCT GCAATGGTTG CGCTCGGAGC TGCCCTACGT GCTGGAAATG 
GTGGCCGAGC TGGCAGGACA GCAGGACCCG GGGCTGGGTG CCTTTTCCTG TCAGGAGGCC 
CGGAAAGCCT GGCTGGACCG TCATGGCAAT CTTGATGAAG CTGTGGAAGA GTGTGTGAGG 
GCCCGGCGGA GGAAGGTGCA GGAACTCCGG TCCCTGGGCT TTGGGCCCGA GGAGGGGTCT 
CTTCAAGCAT TGTTCCAACA TGGTGGTGAT GTGGCACGGG CCCTGACAGA ACTACAGCGC 
CAGCGCCTAG AGCCCTTCCA TCAGCGCCTC TGGGACAAGG GCCTTGATCC CACTCCTTCC 
TGGGATGGGC CAGATAAGCA GAGTCTGGTC AGACGGCTTT TGGCAGTCTA CGCCCTCCCC 
AGCTGGGGCC GAGCAGAGCT GGCACTGTCA CTGCTGCAGG AGACACCCAG GAATTATGAG 
CTGGGAGATG TGGTGGAAGC TGTGAGGCAC AGCCAGGACC GGGCCTTCCT GCGCCGCTTG 
CTTGCCCAGG AGTGTGCCGT GTGTGGCTGG GCCCTGCCCC GCAACCGGAT GCAGGCCCTG 
ACTTCCTGTG AGTGCACCAT CTGTCCCGAC TGCTTCCGCC AGCACTTCAC CATCGCCCTG 
AAGGAGAAAC ACATCACAGA CATGGTGTGC CCCGCCTGTG GCCGCCCTGA CCTCACCGAT 
GACACGCAGT TGCTCAGCTA CTTTTCTACA CTTGACATCC AGCTTCGGGA GAGCCTGGAG 
CCAGATGCCT ATGCACTGTT TCACAAGAAG CTGACTGAGG GTGTGCTCAT GCGGGACCCC 
AAATTCTTGT GGTGTGCCCA ATGCTCCTTT GGCTTCATAT ATGAACGGGA GCAGCTGGAG 
GCAACATGTC CCCAGTGTCA CCAGACCTTC TGTGTGCGCT GTAAGCGCCA GTGGGAGGAG 
CAGCACCGAG GCCGGAGCTG TGAGGATTTC CAGAACTGGA AACGCAACAA TGACCCCGAA 
TACCAGGCCC AGGGCCTAGC CCTGTATCTT CAGGAGAATG GCATCGACTG CCCCAAGTGC 
AAGTTCTCGT ATGCACTGGC CCGAGGAGGC TGCATGCACT TTCACTGCAC CCAATGCCGC 
CACCAGTTCT GCAGCGGCTG CTACAATGCC TTTTACGCCA AGAATAAATG TCCAGACCCT 
AACTGCAGGG TGAAAAAGTC CCTGCATGGC CACCACCCAC GAGACTGCCT CTTTTACCTG 
CGGGACTGGA CTGCTCTCCG GCTCCAGAAG CTGCTACAGG ACAATAATGT CATGTTCAAC 
ACAGAACCCC CAGCAGGGGC CCGGGCAGTT CCTGGCGGTG GCTGCCGAGT GATGGAGCAG 
AAGGAAGTCC CTAATGGGTT CCGGGATGAA GCTTGTGGCA AGGAGACTCC AGCTGGCCAT 
GCTGGCCTCT GCCAGGCACA CTACAAAGAG TATCTTGTGA GCCTCATCAA CGCGCACTCA 
CTGGACCCAG CCACCCTATA TGAGGTGGAG GAGCTGGAGA CGGCTGCTGA GCGCTACCTG 
CACGTGCGCC CTCAGCCAGT GGCAGGGGAG GATGCCCCTG CCTACCATGC CCGCTTGTTA 
CAGAAGCTGA TGGAAGAGGT GCCCTTGGGA CAGAGTATCC CTCGCAGGAG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005623313.1
CDS	265..3501
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDVRKILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVMEQKEVPNGFRDEACGKETPAGHAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETAAERYLHVRPQPVAGEDAPAYHARLLQKLMEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	XM_005623256.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005623256.2

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATGTAAGGAA GATACTGCAG CTCTCAGAGT TTGCCCCCTT ACTGAGAGAG 
ATTGCTCCTG GCCCCCTCAC CACACCCTCT GCCCCAGGCT CCACTCCTGG TCCCTGCTTC 
CTCTGTGGTT CTGCCCCAGG CACACTGCAC TGTTCAACCT GTAAACAGGC CTTGTGTCCA 
GCTTGTGATC GCCTATTCCA TGGACACCCG TCCCGTGCCC ATCACCTCCG TCAGACCCTG 
CCGGGGGGCT CCCAAACCAC CCACCTGACT CCCAGCTTAC CTGCCTTAGC TCCACTGCGG 
CCTCAGTCAG CATCCCTGCT GGCCCTGGGA GACAGCTCTC TTGTGCTTCC CCTGAAAGCT 
TCCCCTGATC CTGCAAATGC CCGTCTGCCC TGGCACTGTG CTGCCTGTGC CATGTTAAAT 
GAATCTTGGG CAGTGCTCTG TGTGGCCTGT GATCGGCCCC GAGGCTGTAA GGGGTTGGGG 
CTGGGGATTG AGGGTTCCCA AGGAACTGGG GGCCTAGAAC CTGAGCTTGC ACGGGGTCGC 
TGGGCCTGCC AGAGCTGCAC CTTTGAGAAT GAGGCAGCAG CTGTGCTGTG TGCCATCTGT 
GAGCGACCTC GGCTGGCTCA GCCTCCTAGC TTGGTGGTGG ATTCTCGGGA TACTGGCATT 
TGCCTGCAGC CCCTTCAGGG GGATACTTTG CTCTCCTCTG CCCAGCCTCC AGTCTGGTAC 
TGTATTCACT GTACCTTCTG CAACTCGGGC CCTGGCTGGG TGTGTGCTAT GTGCAACCGG 
ACCAGCAGCC CCATCCTGGT ACAGCAGGCC CCCCAGCTCC ATGCCAGCTC TTTGGAAGAG 
CGACTTCCTG AGCCACGGCC TCCACGGTGC CTCAGTGCCC CCCTGCCCGG TTACTGTGGG 
GACCCTGAGA AGCAGCGCCA GGACAAGATG CGGGAGGAAG GTCTCCAACT AGTGATGAAG 
ATCCGGGAAG GGGAAGCTGC AGGTGCCTGT CCAGAGGAGG TCTTCTCAGC TCTACAGTAC 
TCAGGCACTG AGGTGCCCCT GCAATGGTTG CGCTCGGAGC TGCCCTACGT GCTGGAAATG 
GTGGCCGAGC TGGCAGGACA GCAGGACCCG GGGCTGGGTG CCTTTTCCTG TCAGGAGGCC 
CGGAAAGCCT GGCTGGACCG TCATGGCAAT CTTGATGAAG CTGTGGAAGA GTGTGTGAGG 
GCCCGGCGGA GGAAGGTGCA GGAACTCCGG TCCCTGGGCT TTGGGCCCGA GGAGGGGTCT 
CTTCAAGCAT TGTTCCAACA TGGTGGTGAT GTGGCACGGG CCCTGACAGA ACTACAGCGC 
CAGCGCCTAG AGCCCTTCCA TCAGCGCCTC TGGGACAAGG GCCTTGATCC CACTCCTTCC 
TGGGATGGGC CAGATAAGCA GAGTCTGGTC AGACGGCTTT TGGCAGTCTA CGCCCTCCCC 
AGCTGGGGCC GAGCAGAGCT GGCACTGTCA CTGCTGCAGG AGACACCCAG GAATTATGAG 
CTGGGAGATG TGGTGGAAGC TGTGAGGCAC AGCCAGGACC GGGCCTTCCT GCGCCGCTTG 
CTTGCCCAGG AGTGTGCCGT GTGTGGCTGG GCCCTGCCCC GCAACCGGAT GCAGGCCCTG 
ACTTCCTGTG AGTGCACCAT CTGTCCCGAC TGCTTCCGCC AGCACTTCAC CATCGCCCTG 
AAGGAGAAAC ACATCACAGA CATGGTGTGC CCCGCCTGTG GCCGCCCTGA CCTCACCGAT 
GACACGCAGT TGCTCAGCTA CTTTTCTACA CTTGACATCC AGCTTCGGGA GAGCCTGGAG 
CCAGATGCCT ATGCACTGTT TCACAAGAAG CTGACTGAGG GTGTGCTCAT GCGGGACCCC 
AAATTCTTGT GGTGTGCCCA ATGCTCCTTT GGCTTCATAT ATGAACGGGA GCAGCTGGAG 
GCAACATGTC CCCAGTGTCA CCAGACCTTC TGTGTGCGCT GTAAGCGCCA GTGGGAGGAG 
CAGCACCGAG GCCGGAGCTG TGAGGATTTC CAGAACTGGA AACGCAACAA TGACCCCGAA 
TACCAGGCCC AGGGCCTAGC CCTGTATCTT CAGGAGAATG GCATCGACTG CCCCAAGTGC 
AAGTTCTCGT ATGCACTGGC CCGAGGAGGC TGCATGCACT TTCACTGCAC CCAATGCCGC 
CACCAGTTCT GCAGCGGCTG CTACAATGCC TTTTACGCCA AGAATAAATG TCCAGACCCT 
AACTGCAGGG TGAAAAAGTC CCTGCATGGC CACCACCCAC GAGACTGCCT CTTTTACCTG 
CGGGACTGGA CTGCTCTCCG GCTCCAGAAG CTGCTACAGG ACAATAATGT CATGTTCAAC 
ACAGAACCCC CAGCAGGGGC CCGGGCAGTT CCTGGCGGTG GCTGCCGAGT GATGGAGCAG 
AAGGAAGTCC CTAATGGGTT CCGGGATGAA GCTTGTGGCA AGGAGACTCC AGCTGGCCAT 
GCTGGCCTCT GCCAGGCACA CTACAAAGAG TATCTTGTGA GCCTCATCAA CGCGCACTCA 
CTGGACCCAG CCACCCTATA TGAGGTGGAG GAGCTGGAGA CGGCTGCTGA GCGCTACCTG 
CACGTGCGCC CTCAGCCAGT GGCAGGGGAG GATGCCCCTG CCTACCATGC CCGCTTGTTA 
CAGAAGCTGA TGGAAGAGGT GCCCTTGGGA CAGAGTATCC CTCGCAGGAG GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_537383.6 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_537383.2 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X3, mRNA.

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CloneID	OCe10191
Clone ID Related Accession (Same CDS sequence)	XM_537383.6 , XM_537383.5
Accession Version	XM_537383.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537383.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATATACTGCA GCTCTCAGAG TTTGCCCCCT TACTGAGAGA GATTGCTCCT 
GGCCCCCTCA CCACACCCTC TGCCCCAGGC TCCACTCCTG GTCCCTGCTT CCTCTGTGGT 
TCTGCCCCAG GCACACTGCA CTGTTCAACC TGTAAACAGG CCTTGTGTCC AGCTTGTGAT 
CGCCTATTCC ATGGACACCC GTCCCGTGCC CATCACCTCC GTCAGACCCT GCCGGGGGGC 
TCCCAAACCA CCCACCTGAC TCCCAGCTTA CCTGCCTTAG CTCCACTGCG GCCTCAGTCA 
GCATCCCTGC TGGCCCTGGG AGACAGCTCT CTTGTGCTTC CCCTGAAAGC TTCCCCTGAT 
CCTGCAAATG CCCGTCTGCC CTGGCACTGT GCTGCCTGTG CCATGTTAAA TGAATCTTGG 
GCAGTGCTCT GTGTGGCCTG TGATCGGCCC CGAGGCTGTA AGGGGTTGGG GCTGGGGATT 
GAGGGTTCCC AAGGAACTGG GGGCCTAGAA CCTGAGCTTG CACGGGGTCG CTGGGCCTGC 
CAGAGCTGCA CCTTTGAGAA TGAGGCAGCA GCTGTGCTGT GTGCCATCTG TGAGCGACCT 
CGGCTGGCTC AGCCTCCTAG CTTGGTGGTG GATTCTCGGG ATACTGGCAT TTGCCTGCAG 
CCCCTTCAGC AGGGGGATAC TTTGCTCTCC TCTGCCCAGC CTCCAGTCTG GTACTGTATT 
CACTGTACCT TCTGCAACTC GGGCCCTGGC TGGGTGTGTG CTATGTGCAA CCGGACCAGC 
AGCCCCATCC TGGTACAGCA GGCCCCCCAG CTCCATGCCA GCTCTTTGGA AGAGCGACTT 
CCTGAGCCAC GGCCTCCACG GTGCCTCAGT GCCCCCCTGC CCGGTTACTG TGGGGACCCT 
GAGAAGCAGC GCCAGGACAA GATGCGGGAG GAAGGTCTCC AACTAGTGAT GAAGATCCGG 
GAAGGGGAAG CTGCAGGTGC CTGTCCAGAG GAGGTCTTCT CAGCTCTACA GTACTCAGGC 
ACTGAGGTGC CCCTGCAATG GTTGCGCTCG GAGCTGCCCT ACGTGCTGGA AATGGTGGCC 
GAGCTGGCAG GACAGCAGGA CCCGGGGCTG GGTGCCTTTT CCTGTCAGGA GGCCCGGAAA 
GCCTGGCTGG ACCGTCATGG CAATCTTGAT GAAGCTGTGG AAGAGTGTGT GAGGGCCCGG 
CGGAGGAAGG TGCAGGAACT CCGGTCCCTG GGCTTTGGGC CCGAGGAGGG GTCTCTTCAA 
GCATTGTTCC AACATGGTGG TGATGTGGCA CGGGCCCTGA CAGAACTACA GCGCCAGCGC 
CTAGAGCCCT TCCATCAGCG CCTCTGGGAC AAGGGCCTTG ATCCCACTCC TTCCTGGGAT 
GGGCCAGATA AGCAGAGTCT GGTCAGACGG CTTTTGGCAG TCTACGCCCT CCCCAGCTGG 
GGCCGAGCAG AGCTGGCACT GTCACTGCTG CAGGAGACAC CCAGGAATTA TGAGCTGGGA 
GATGTGGTGG AAGCTGTGAG GCACAGCCAG GACCGGGCCT TCCTGCGCCG CTTGCTTGCC 
CAGGAGTGTG CCGTGTGTGG CTGGGCCCTG CCCCGCAACC GGATGCAGGC CCTGACTTCC 
TGTGAGTGCA CCATCTGTCC CGACTGCTTC CGCCAGCACT TCACCATCGC CCTGAAGGAG 
AAACACATCA CAGACATGGT GTGCCCCGCC TGTGGCCGCC CTGACCTCAC CGATGACACG 
CAGTTGCTCA GCTACTTTTC TACACTTGAC ATCCAGCTTC GGGAGAGCCT GGAGCCAGAT 
GCCTATGCAC TGTTTCACAA GAAGCTGACT GAGGGTGTGC TCATGCGGGA CCCCAAATTC 
TTGTGGTGTG CCCAATGCTC CTTTGGCTTC ATATATGAAC GGGAGCAGCT GGAGGCAACA 
TGTCCCCAGT GTCACCAGAC CTTCTGTGTG CGCTGTAAGC GCCAGTGGGA GGAGCAGCAC 
CGAGGCCGGA GCTGTGAGGA TTTCCAGAAC TGGAAACGCA ACAATGACCC CGAATACCAG 
GCCCAGGGCC TAGCCCTGTA TCTTCAGGAG AATGGCATCG ACTGCCCCAA GTGCAAGTTC 
TCGTATGCAC TGGCCCGAGG AGGCTGCATG CACTTTCACT GCACCCAATG CCGCCACCAG 
TTCTGCAGCG GCTGCTACAA TGCCTTTTAC GCCAAGAATA AATGTCCAGA CCCTAACTGC 
AGGGTGAAAA AGTCCCTGCA TGGCCACCAC CCACGAGACT GCCTCTTTTA CCTGCGGGAC 
TGGACTGCTC TCCGGCTCCA GAAGCTGCTA CAGGACAATA ATGTCATGTT CAACACAGAA 
CCCCCAGCAG GGGCCCGGGC AGTTCCTGGC GGTGGCTGCC GAGTGATGGA GCAGAAGGAA 
GTCCCTAATG GGTTCCGGGA TGAAGCTTGT GGCAAGGAGA CTCCAGCTGG CCATGCTGGC 
CTCTGCCAGG CACACTACAA AGAGTATCTT GTGAGCCTCA TCAACGCGCA CTCACTGGAC 
CCAGCCACCC TATATGAGGT GGAGGAGCTG GAGACGGCTG CTGAGCGCTA CCTGCACGTG 
CGCCCTCAGC CAGTGGCAGG GGAGGATGCC CCTGCCTACC ATGCCCGCTT GTTACAGAAG 
CTGATGGAAG AGGTGCCCTT GGGACAGAGT ATCCCTCGCA GGAGGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537383.2
CDS	256..3486
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVMEQKEVPNGFRDEACGKETPAGHAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETAAERYLHVRPQPVAGEDAPAYHARLLQKLMEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	XM_537383.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537383.6

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATATACTGCA GCTCTCAGAG TTTGCCCCCT TACTGAGAGA GATTGCTCCT 
GGCCCCCTCA CCACACCCTC TGCCCCAGGC TCCACTCCTG GTCCCTGCTT CCTCTGTGGT 
TCTGCCCCAG GCACACTGCA CTGTTCAACC TGTAAACAGG CCTTGTGTCC AGCTTGTGAT 
CGCCTATTCC ATGGACACCC GTCCCGTGCC CATCACCTCC GTCAGACCCT GCCGGGGGGC 
TCCCAAACCA CCCACCTGAC TCCCAGCTTA CCTGCCTTAG CTCCACTGCG GCCTCAGTCA 
GCATCCCTGC TGGCCCTGGG AGACAGCTCT CTTGTGCTTC CCCTGAAAGC TTCCCCTGAT 
CCTGCAAATG CCCGTCTGCC CTGGCACTGT GCTGCCTGTG CCATGTTAAA TGAATCTTGG 
GCAGTGCTCT GTGTGGCCTG TGATCGGCCC CGAGGCTGTA AGGGGTTGGG GCTGGGGATT 
GAGGGTTCCC AAGGAACTGG GGGCCTAGAA CCTGAGCTTG CACGGGGTCG CTGGGCCTGC 
CAGAGCTGCA CCTTTGAGAA TGAGGCAGCA GCTGTGCTGT GTGCCATCTG TGAGCGACCT 
CGGCTGGCTC AGCCTCCTAG CTTGGTGGTG GATTCTCGGG ATACTGGCAT TTGCCTGCAG 
CCCCTTCAGC AGGGGGATAC TTTGCTCTCC TCTGCCCAGC CTCCAGTCTG GTACTGTATT 
CACTGTACCT TCTGCAACTC GGGCCCTGGC TGGGTGTGTG CTATGTGCAA CCGGACCAGC 
AGCCCCATCC TGGTACAGCA GGCCCCCCAG CTCCATGCCA GCTCTTTGGA AGAGCGACTT 
CCTGAGCCAC GGCCTCCACG GTGCCTCAGT GCCCCCCTGC CCGGTTACTG TGGGGACCCT 
GAGAAGCAGC GCCAGGACAA GATGCGGGAG GAAGGTCTCC AACTAGTGAT GAAGATCCGG 
GAAGGGGAAG CTGCAGGTGC CTGTCCAGAG GAGGTCTTCT CAGCTCTACA GTACTCAGGC 
ACTGAGGTGC CCCTGCAATG GTTGCGCTCG GAGCTGCCCT ACGTGCTGGA AATGGTGGCC 
GAGCTGGCAG GACAGCAGGA CCCGGGGCTG GGTGCCTTTT CCTGTCAGGA GGCCCGGAAA 
GCCTGGCTGG ACCGTCATGG CAATCTTGAT GAAGCTGTGG AAGAGTGTGT GAGGGCCCGG 
CGGAGGAAGG TGCAGGAACT CCGGTCCCTG GGCTTTGGGC CCGAGGAGGG GTCTCTTCAA 
GCATTGTTCC AACATGGTGG TGATGTGGCA CGGGCCCTGA CAGAACTACA GCGCCAGCGC 
CTAGAGCCCT TCCATCAGCG CCTCTGGGAC AAGGGCCTTG ATCCCACTCC TTCCTGGGAT 
GGGCCAGATA AGCAGAGTCT GGTCAGACGG CTTTTGGCAG TCTACGCCCT CCCCAGCTGG 
GGCCGAGCAG AGCTGGCACT GTCACTGCTG CAGGAGACAC CCAGGAATTA TGAGCTGGGA 
GATGTGGTGG AAGCTGTGAG GCACAGCCAG GACCGGGCCT TCCTGCGCCG CTTGCTTGCC 
CAGGAGTGTG CCGTGTGTGG CTGGGCCCTG CCCCGCAACC GGATGCAGGC CCTGACTTCC 
TGTGAGTGCA CCATCTGTCC CGACTGCTTC CGCCAGCACT TCACCATCGC CCTGAAGGAG 
AAACACATCA CAGACATGGT GTGCCCCGCC TGTGGCCGCC CTGACCTCAC CGATGACACG 
CAGTTGCTCA GCTACTTTTC TACACTTGAC ATCCAGCTTC GGGAGAGCCT GGAGCCAGAT 
GCCTATGCAC TGTTTCACAA GAAGCTGACT GAGGGTGTGC TCATGCGGGA CCCCAAATTC 
TTGTGGTGTG CCCAATGCTC CTTTGGCTTC ATATATGAAC GGGAGCAGCT GGAGGCAACA 
TGTCCCCAGT GTCACCAGAC CTTCTGTGTG CGCTGTAAGC GCCAGTGGGA GGAGCAGCAC 
CGAGGCCGGA GCTGTGAGGA TTTCCAGAAC TGGAAACGCA ACAATGACCC CGAATACCAG 
GCCCAGGGCC TAGCCCTGTA TCTTCAGGAG AATGGCATCG ACTGCCCCAA GTGCAAGTTC 
TCGTATGCAC TGGCCCGAGG AGGCTGCATG CACTTTCACT GCACCCAATG CCGCCACCAG 
TTCTGCAGCG GCTGCTACAA TGCCTTTTAC GCCAAGAATA AATGTCCAGA CCCTAACTGC 
AGGGTGAAAA AGTCCCTGCA TGGCCACCAC CCACGAGACT GCCTCTTTTA CCTGCGGGAC 
TGGACTGCTC TCCGGCTCCA GAAGCTGCTA CAGGACAATA ATGTCATGTT CAACACAGAA 
CCCCCAGCAG GGGCCCGGGC AGTTCCTGGC GGTGGCTGCC GAGTGATGGA GCAGAAGGAA 
GTCCCTAATG GGTTCCGGGA TGAAGCTTGT GGCAAGGAGA CTCCAGCTGG CCATGCTGGC 
CTCTGCCAGG CACACTACAA AGAGTATCTT GTGAGCCTCA TCAACGCGCA CTCACTGGAC 
CCAGCCACCC TATATGAGGT GGAGGAGCTG GAGACGGCTG CTGAGCGCTA CCTGCACGTG 
CGCCCTCAGC CAGTGGCAGG GGAGGATGCC CCTGCCTACC ATGCCCGCTT GTTACAGAAG 
CTGATGGAAG AGGTGCCCTT GGGACAGAGT ATCCCTCGCA GGAGGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF31 ( XM_537383.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_537383.2 Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X3, mRNA.

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CloneID	OCe10191
Clone ID Related Accession (Same CDS sequence)	XM_537383.6 , XM_537383.5
Accession Version	XM_537383.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RNF31 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537383.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATATACTGCA GCTCTCAGAG TTTGCCCCCT TACTGAGAGA GATTGCTCCT 
GGCCCCCTCA CCACACCCTC TGCCCCAGGC TCCACTCCTG GTCCCTGCTT CCTCTGTGGT 
TCTGCCCCAG GCACACTGCA CTGTTCAACC TGTAAACAGG CCTTGTGTCC AGCTTGTGAT 
CGCCTATTCC ATGGACACCC GTCCCGTGCC CATCACCTCC GTCAGACCCT GCCGGGGGGC 
TCCCAAACCA CCCACCTGAC TCCCAGCTTA CCTGCCTTAG CTCCACTGCG GCCTCAGTCA 
GCATCCCTGC TGGCCCTGGG AGACAGCTCT CTTGTGCTTC CCCTGAAAGC TTCCCCTGAT 
CCTGCAAATG CCCGTCTGCC CTGGCACTGT GCTGCCTGTG CCATGTTAAA TGAATCTTGG 
GCAGTGCTCT GTGTGGCCTG TGATCGGCCC CGAGGCTGTA AGGGGTTGGG GCTGGGGATT 
GAGGGTTCCC AAGGAACTGG GGGCCTAGAA CCTGAGCTTG CACGGGGTCG CTGGGCCTGC 
CAGAGCTGCA CCTTTGAGAA TGAGGCAGCA GCTGTGCTGT GTGCCATCTG TGAGCGACCT 
CGGCTGGCTC AGCCTCCTAG CTTGGTGGTG GATTCTCGGG ATACTGGCAT TTGCCTGCAG 
CCCCTTCAGC AGGGGGATAC TTTGCTCTCC TCTGCCCAGC CTCCAGTCTG GTACTGTATT 
CACTGTACCT TCTGCAACTC GGGCCCTGGC TGGGTGTGTG CTATGTGCAA CCGGACCAGC 
AGCCCCATCC TGGTACAGCA GGCCCCCCAG CTCCATGCCA GCTCTTTGGA AGAGCGACTT 
CCTGAGCCAC GGCCTCCACG GTGCCTCAGT GCCCCCCTGC CCGGTTACTG TGGGGACCCT 
GAGAAGCAGC GCCAGGACAA GATGCGGGAG GAAGGTCTCC AACTAGTGAT GAAGATCCGG 
GAAGGGGAAG CTGCAGGTGC CTGTCCAGAG GAGGTCTTCT CAGCTCTACA GTACTCAGGC 
ACTGAGGTGC CCCTGCAATG GTTGCGCTCG GAGCTGCCCT ACGTGCTGGA AATGGTGGCC 
GAGCTGGCAG GACAGCAGGA CCCGGGGCTG GGTGCCTTTT CCTGTCAGGA GGCCCGGAAA 
GCCTGGCTGG ACCGTCATGG CAATCTTGAT GAAGCTGTGG AAGAGTGTGT GAGGGCCCGG 
CGGAGGAAGG TGCAGGAACT CCGGTCCCTG GGCTTTGGGC CCGAGGAGGG GTCTCTTCAA 
GCATTGTTCC AACATGGTGG TGATGTGGCA CGGGCCCTGA CAGAACTACA GCGCCAGCGC 
CTAGAGCCCT TCCATCAGCG CCTCTGGGAC AAGGGCCTTG ATCCCACTCC TTCCTGGGAT 
GGGCCAGATA AGCAGAGTCT GGTCAGACGG CTTTTGGCAG TCTACGCCCT CCCCAGCTGG 
GGCCGAGCAG AGCTGGCACT GTCACTGCTG CAGGAGACAC CCAGGAATTA TGAGCTGGGA 
GATGTGGTGG AAGCTGTGAG GCACAGCCAG GACCGGGCCT TCCTGCGCCG CTTGCTTGCC 
CAGGAGTGTG CCGTGTGTGG CTGGGCCCTG CCCCGCAACC GGATGCAGGC CCTGACTTCC 
TGTGAGTGCA CCATCTGTCC CGACTGCTTC CGCCAGCACT TCACCATCGC CCTGAAGGAG 
AAACACATCA CAGACATGGT GTGCCCCGCC TGTGGCCGCC CTGACCTCAC CGATGACACG 
CAGTTGCTCA GCTACTTTTC TACACTTGAC ATCCAGCTTC GGGAGAGCCT GGAGCCAGAT 
GCCTATGCAC TGTTTCACAA GAAGCTGACT GAGGGTGTGC TCATGCGGGA CCCCAAATTC 
TTGTGGTGTG CCCAATGCTC CTTTGGCTTC ATATATGAAC GGGAGCAGCT GGAGGCAACA 
TGTCCCCAGT GTCACCAGAC CTTCTGTGTG CGCTGTAAGC GCCAGTGGGA GGAGCAGCAC 
CGAGGCCGGA GCTGTGAGGA TTTCCAGAAC TGGAAACGCA ACAATGACCC CGAATACCAG 
GCCCAGGGCC TAGCCCTGTA TCTTCAGGAG AATGGCATCG ACTGCCCCAA GTGCAAGTTC 
TCGTATGCAC TGGCCCGAGG AGGCTGCATG CACTTTCACT GCACCCAATG CCGCCACCAG 
TTCTGCAGCG GCTGCTACAA TGCCTTTTAC GCCAAGAATA AATGTCCAGA CCCTAACTGC 
AGGGTGAAAA AGTCCCTGCA TGGCCACCAC CCACGAGACT GCCTCTTTTA CCTGCGGGAC 
TGGACTGCTC TCCGGCTCCA GAAGCTGCTA CAGGACAATA ATGTCATGTT CAACACAGAA 
CCCCCAGCAG GGGCCCGGGC AGTTCCTGGC GGTGGCTGCC GAGTGATGGA GCAGAAGGAA 
GTCCCTAATG GGTTCCGGGA TGAAGCTTGT GGCAAGGAGA CTCCAGCTGG CCATGCTGGC 
CTCTGCCAGG CACACTACAA AGAGTATCTT GTGAGCCTCA TCAACGCGCA CTCACTGGAC 
CCAGCCACCC TATATGAGGT GGAGGAGCTG GAGACGGCTG CTGAGCGCTA CCTGCACGTG 
CGCCCTCAGC CAGTGGCAGG GGAGGATGCC CCTGCCTACC ATGCCCGCTT GTTACAGAAG 
CTGATGGAAG AGGTGCCCTT GGGACAGAGT ATCCCTCGCA GGAGGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537383.2
CDS	265..3495
Translation	MPGEEERAFLAAREELSSALRKDSGQVFSVEQLRPLLVTSLPPAARYLQLDAARLVRCNAHGEPRNYLNTLSTALNILEKYGRNLLSPQRPRYWRGVKFNNPVFRSTVDAVQGGRDVLRLYGYTEEQPDGLSFPEGQEEPDEHQVATVTLEVLLLRTELSLLLQNTHPRQQALEQLLEDKVEDDILQLSEFAPLLREIAPGPLTTPSAPGSTPGPCFLCGSAPGTLHCSTCKQALCPACDRLFHGHPSRAHHLRQTLPGGSQTTHLTPSLPALAPLRPQSASLLALGDSSLVLPLKASPDPANARLPWHCAACAMLNESWAVLCVACDRPRGCKGLGLGIEGSQGTGGLEPELARGRWACQSCTFENEAAAVLCAICERPRLAQPPSLVVDSRDTGICLQPLQQGDTLLSSAQPPVWYCIHCTFCNSGPGWVCAMCNRTSSPILVQQAPQLHASSLEERLPEPRPPRCLSAPLPGYCGDPEKQRQDKMREEGLQLVMKIREGEAAGACPEEVFSALQYSGTEVPLQWLRSELPYVLEMVAELAGQQDPGLGAFSCQEARKAWLDRHGNLDEAVEECVRARRRKVQELRSLGFGPEEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDKGLDPTPSWDGPDKQSLVRRLLAVYALPSWGRAELALSLLQETPRNYELGDVVEAVRHSQDRAFLRRLLAQECAVCGWALPRNRMQALTSCECTICPDCFRQHFTIALKEKHITDMVCPACGRPDLTDDTQLLSYFSTLDIQLRESLEPDAYALFHKKLTEGVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQNWKRNNDPEYQAQGLALYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNCRVKKSLHGHHPRDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVMEQKEVPNGFRDEACGKETPAGHAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETAAERYLHVRPQPVAGEDAPAYHARLLQKLMEEVPLGQSIPRRRK

Target ORF information:

RefSeq Version	XM_537383.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger protein 31 (RNF31), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537383.5

ORF Insert Sequence:

ATGCCGGGGG AGGAGGAGCG GGCCTTCCTG GCGGCCCGCG AGGAGCTGTC GAGCGCCCTG 
AGGAAGGATT CCGGGCAGGT GTTTTCCGTA GAGCAGCTCC GGCCGCTATT GGTCACATCC 
CTGCCGCCAG CGGCCCGCTA CCTGCAGCTG GACGCCGCAC GCCTGGTCCG CTGCAATGCT 
CATGGGGAGC CCCGAAACTA CCTCAACACC CTGTCCACGG CCCTGAACAT CCTGGAGAAA 
TATGGCCGCA ACCTTCTCAG CCCTCAGCGG CCCCGGTATT GGCGCGGGGT CAAGTTCAAT 
AACCCTGTCT TTCGCAGTAC GGTGGATGCT GTGCAGGGGG GCCGGGACGT TCTGCGATTG 
TATGGCTACA CAGAGGAGCA GCCAGATGGG TTGAGTTTCC CTGAGGGGCA GGAGGAGCCA 
GATGAGCACC AAGTAGCTAC AGTCACACTA GAAGTACTGC TGCTTCGGAC AGAGCTCAGC 
CTGCTGTTAC AGAATACTCA CCCAAGACAA CAAGCACTGG AGCAGCTGCT GGAAGACAAG 
GTTGAGGATG ATATACTGCA GCTCTCAGAG TTTGCCCCCT TACTGAGAGA GATTGCTCCT 
GGCCCCCTCA CCACACCCTC TGCCCCAGGC TCCACTCCTG GTCCCTGCTT CCTCTGTGGT 
TCTGCCCCAG GCACACTGCA CTGTTCAACC TGTAAACAGG CCTTGTGTCC AGCTTGTGAT 
CGCCTATTCC ATGGACACCC GTCCCGTGCC CATCACCTCC GTCAGACCCT GCCGGGGGGC 
TCCCAAACCA CCCACCTGAC TCCCAGCTTA CCTGCCTTAG CTCCACTGCG GCCTCAGTCA 
GCATCCCTGC TGGCCCTGGG AGACAGCTCT CTTGTGCTTC CCCTGAAAGC TTCCCCTGAT 
CCTGCAAATG CCCGTCTGCC CTGGCACTGT GCTGCCTGTG CCATGTTAAA TGAATCTTGG 
GCAGTGCTCT GTGTGGCCTG TGATCGGCCC CGAGGCTGTA AGGGGTTGGG GCTGGGGATT 
GAGGGTTCCC AAGGAACTGG GGGCCTAGAA CCTGAGCTTG CACGGGGTCG CTGGGCCTGC 
CAGAGCTGCA CCTTTGAGAA TGAGGCAGCA GCTGTGCTGT GTGCCATCTG TGAGCGACCT 
CGGCTGGCTC AGCCTCCTAG CTTGGTGGTG GATTCTCGGG ATACTGGCAT TTGCCTGCAG 
CCCCTTCAGC AGGGGGATAC TTTGCTCTCC TCTGCCCAGC CTCCAGTCTG GTACTGTATT 
CACTGTACCT TCTGCAACTC GGGCCCTGGC TGGGTGTGTG CTATGTGCAA CCGGACCAGC 
AGCCCCATCC TGGTACAGCA GGCCCCCCAG CTCCATGCCA GCTCTTTGGA AGAGCGACTT 
CCTGAGCCAC GGCCTCCACG GTGCCTCAGT GCCCCCCTGC CCGGTTACTG TGGGGACCCT 
GAGAAGCAGC GCCAGGACAA GATGCGGGAG GAAGGTCTCC AACTAGTGAT GAAGATCCGG 
GAAGGGGAAG CTGCAGGTGC CTGTCCAGAG GAGGTCTTCT CAGCTCTACA GTACTCAGGC 
ACTGAGGTGC CCCTGCAATG GTTGCGCTCG GAGCTGCCCT ACGTGCTGGA AATGGTGGCC 
GAGCTGGCAG GACAGCAGGA CCCGGGGCTG GGTGCCTTTT CCTGTCAGGA GGCCCGGAAA 
GCCTGGCTGG ACCGTCATGG CAATCTTGAT GAAGCTGTGG AAGAGTGTGT GAGGGCCCGG 
CGGAGGAAGG TGCAGGAACT CCGGTCCCTG GGCTTTGGGC CCGAGGAGGG GTCTCTTCAA 
GCATTGTTCC AACATGGTGG TGATGTGGCA CGGGCCCTGA CAGAACTACA GCGCCAGCGC 
CTAGAGCCCT TCCATCAGCG CCTCTGGGAC AAGGGCCTTG ATCCCACTCC TTCCTGGGAT 
GGGCCAGATA AGCAGAGTCT GGTCAGACGG CTTTTGGCAG TCTACGCCCT CCCCAGCTGG 
GGCCGAGCAG AGCTGGCACT GTCACTGCTG CAGGAGACAC CCAGGAATTA TGAGCTGGGA 
GATGTGGTGG AAGCTGTGAG GCACAGCCAG GACCGGGCCT TCCTGCGCCG CTTGCTTGCC 
CAGGAGTGTG CCGTGTGTGG CTGGGCCCTG CCCCGCAACC GGATGCAGGC CCTGACTTCC 
TGTGAGTGCA CCATCTGTCC CGACTGCTTC CGCCAGCACT TCACCATCGC CCTGAAGGAG 
AAACACATCA CAGACATGGT GTGCCCCGCC TGTGGCCGCC CTGACCTCAC CGATGACACG 
CAGTTGCTCA GCTACTTTTC TACACTTGAC ATCCAGCTTC GGGAGAGCCT GGAGCCAGAT 
GCCTATGCAC TGTTTCACAA GAAGCTGACT GAGGGTGTGC TCATGCGGGA CCCCAAATTC 
TTGTGGTGTG CCCAATGCTC CTTTGGCTTC ATATATGAAC GGGAGCAGCT GGAGGCAACA 
TGTCCCCAGT GTCACCAGAC CTTCTGTGTG CGCTGTAAGC GCCAGTGGGA GGAGCAGCAC 
CGAGGCCGGA GCTGTGAGGA TTTCCAGAAC TGGAAACGCA ACAATGACCC CGAATACCAG 
GCCCAGGGCC TAGCCCTGTA TCTTCAGGAG AATGGCATCG ACTGCCCCAA GTGCAAGTTC 
TCGTATGCAC TGGCCCGAGG AGGCTGCATG CACTTTCACT GCACCCAATG CCGCCACCAG 
TTCTGCAGCG GCTGCTACAA TGCCTTTTAC GCCAAGAATA AATGTCCAGA CCCTAACTGC 
AGGGTGAAAA AGTCCCTGCA TGGCCACCAC CCACGAGACT GCCTCTTTTA CCTGCGGGAC 
TGGACTGCTC TCCGGCTCCA GAAGCTGCTA CAGGACAATA ATGTCATGTT CAACACAGAA 
CCCCCAGCAG GGGCCCGGGC AGTTCCTGGC GGTGGCTGCC GAGTGATGGA GCAGAAGGAA 
GTCCCTAATG GGTTCCGGGA TGAAGCTTGT GGCAAGGAGA CTCCAGCTGG CCATGCTGGC 
CTCTGCCAGG CACACTACAA AGAGTATCTT GTGAGCCTCA TCAACGCGCA CTCACTGGAC 
CCAGCCACCC TATATGAGGT GGAGGAGCTG GAGACGGCTG CTGAGCGCTA CCTGCACGTG 
CGCCCTCAGC CAGTGGCAGG GGAGGATGCC CCTGCCTACC ATGCCCGCTT GTTACAGAAG 
CTGATGGAAG AGGTGCCCTT GGGACAGAGT ATCCCTCGCA GGAGGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RNF31 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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