PRMT5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRMT5
Entrez Gene ID	480242
Full Name	protein arginine methyltransferase 5
General protein information	Preferred Names protein arginine methyltransferase 5 Names protein arginine N-methyltransferase 5
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022421802.1	XP_022277510.1	protein arginine N-methyltransferase 5 isoform X3
XM_852436.4	XP_857529.1	protein arginine N-methyltransferase 5 isoform X4
XM_537366.5	XP_537366.2	protein arginine N-methyltransferase 5 isoform X1
XM_014115755.1	XP_013971230.1	protein arginine N-methyltransferase 5 isoform X2
XM_852436.3	XP_857529.1	protein arginine N-methyltransferase 5 isoform X3
XM_022421801.1	XP_022277509.1	protein arginine N-methyltransferase 5 isoform X1
XM_537366.6	XP_537366.2	protein arginine N-methyltransferase 5 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PRMT5	XP_509844.3
Canis lupus familiaris (dog)	PRMT5	XP_537366.2
Mus musculus (house mouse)	Prmt5	NP_038796.2
Danio rerio (zebrafish)	prmt5	NP_001007184.2
Xenopus tropicalis (tropical clawed frog)	prmt5	XP_002941557.1
Homo sapiens (human)	PRMT5	NP_006100.2
Drosophila melanogaster (fruit fly)	csul	NP_725552.1
Oryza sativa (rice)	Os02g0139200	NP_001045843.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009048	XP_319800.4
	PRMT5	XP_001103964.1
Bos taurus (cattle)	PRMT5	NP_001098844.1
Rattus norvegicus (Norway rat)	Prmt5	NP_001102337.2
Arabidopsis thaliana (thale cress)	SKB1	NP_194841.2

Gene Ontology

The following PRMT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRMT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe10131	XM_537366.6 Latest version!	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe10132	XM_852436.4 Latest version!	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe289980	XM_022421801.1 Latest version!	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe289981	XM_022421802.1 Latest version!	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe37813	XM_014115755.1 Latest version!	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OCe10132	XM_852436.3	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OCe10131	XM_537366.5	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRMT5 ( XM_537366.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537366.2 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe10131
Clone ID Related Accession (Same CDS sequence)	XM_537366.5 , XM_537366.6
Accession Version	XM_537366.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein arginine N-methyltransferase 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537366.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TGGCGGTCGG AGGTGCCGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
AACTGCGTGC CCGAAATAGC TGACACTTTG GGGGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCACTCAGGA ACCTGCTAAG 
AATCGGCCGG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG 
CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATCCGC 
AGGAACTCTG AGGCGGCAAT GTTACAGGAG CTGAATTTTG GGGCATATTT GGGTCTTCCA 
GCTTTCCTGT TGCCCCTAAA TCAGGAAGAT AACACAAACT TGGCCAGGGT TTTGACCAAC 
CACATCCACA CTGGCCACCA CTCGTCCATG TTCTGGATGC GGGTACCCTT GGTGGCTCCA 
GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAAGTA CACATACAGA GGAGTATAGT 
GGAGAGGAGA AGACATGGAT GTGGTGGCAC AACTTCAGGA CCTTGTGTGA CTATAGCAAG 
AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGT 
TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACCA GCATTTTCCT GACCAATAAG 
AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTGA TCTTCCGGCT TCTCAAGTTG 
GAAGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCTTAT 
CTCCAGTACC TGGAATACCT GAGCCAGAAC CGCCCTCCAC CCAATGCCTA TGAACTCTTT 
GCCAAGGGCT ATGAAGACTA TCTGCAGTCT CCACTCCAGC CACTGATGGA CAATCTGGAA 
TCTCAGACTT ATGAAGTGTT TGAAAAGGAC CCTATCAAAT ATTCTCAATA TCAGCAGGCC 
ATCTATAAAT GTCTGTTAGA TCGAGTGCCA GATGAGGAGA AGGATACCAA TATCCAGGTG 
CTAATGGTGC TGGGAGCAGG CCGGGGTCCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG 
CAGGCTGACC GGCGGATAAA GCTGTATGCT GTGGAGAAGA ATCCAAATGC TGTGGTGACG 
CTAGAGAACT GGCAATTTGA AGAATGGGGA AGCCAGGTGA CAGTAGTCTC ATCGGACATG 
CGGGAATGGG TGGCTCCAGA GAAGGCAGAC ATCATCGTCA GTGAGCTTCT AGGCTCCTTC 
GCTGACAATG AACTGTCACC TGAGTGCCTG GATGGAGCCC AACACTTCCT AAAAGATGAT 
GGTGTGAGCA TCCCTGGGGA GTACACCTCC TTTCTGGCTC CCATCTCCTC CTCCAAGTTA 
TACAATGAGG TCAGAGCCTG TCGAGAAAAG GACCGAGACC CTGAGGCCCA GTTCGAGATG 
CCTTATGTGG TGCGGCTGCA CAACTTCCAC CAGTTGTCTG CGCCCCAGCC CTGTTTCACC 
TTCAGCCATC CCAATAGAGA TCCTATGATT GACAACAACC GCTACTGCAC CTTGGAGTTT 
CCCGTGGAGG TGAATACAGT GCTACATGGC TTTGCAGGCT ACTTTGAGAC TGTGCTTTAT 
CAGGACATCA CCCTAAGTAT CCGTCCAGAA ACTCACTCTC CTGGGATGTT CTCATGGTTT 
CCCATCCTCT TCCCCATCAA GCAGCCCATT ACAGTACGTG AAGGCCAGAC CATCTGTGTG 
CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA 
GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATACA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537366.2
CDS	128..2041
Translation	MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFTQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_537366.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537366.5

ORF Insert Sequence:

ATGGCGGCGA TGGCGGTCGG AGGTGCCGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
AACTGCGTGC CCGAAATAGC TGACACTTTG GGGGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCACTCAGGA ACCTGCTAAG 
AATCGGCCGG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG 
CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATCCGC 
AGGAACTCTG AGGCGGCAAT GTTACAGGAG CTGAATTTTG GGGCATATTT GGGTCTTCCA 
GCTTTCCTGT TGCCCCTAAA TCAGGAAGAT AACACAAACT TGGCCAGGGT TTTGACCAAC 
CACATCCACA CTGGCCACCA CTCGTCCATG TTCTGGATGC GGGTACCCTT GGTGGCTCCA 
GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAAGTA CACATACAGA GGAGTATAGT 
GGAGAGGAGA AGACATGGAT GTGGTGGCAC AACTTCAGGA CCTTGTGTGA CTATAGCAAG 
AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGT 
TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACCA GCATTTTCCT GACCAATAAG 
AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTGA TCTTCCGGCT TCTCAAGTTG 
GAAGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCTTAT 
CTCCAGTACC TGGAATACCT GAGCCAGAAC CGCCCTCCAC CCAATGCCTA TGAACTCTTT 
GCCAAGGGCT ATGAAGACTA TCTGCAGTCT CCACTCCAGC CACTGATGGA CAATCTGGAA 
TCTCAGACTT ATGAAGTGTT TGAAAAGGAC CCTATCAAAT ATTCTCAATA TCAGCAGGCC 
ATCTATAAAT GTCTGTTAGA TCGAGTGCCA GATGAGGAGA AGGATACCAA TATCCAGGTG 
CTAATGGTGC TGGGAGCAGG CCGGGGTCCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG 
CAGGCTGACC GGCGGATAAA GCTGTATGCT GTGGAGAAGA ATCCAAATGC TGTGGTGACG 
CTAGAGAACT GGCAATTTGA AGAATGGGGA AGCCAGGTGA CAGTAGTCTC ATCGGACATG 
CGGGAATGGG TGGCTCCAGA GAAGGCAGAC ATCATCGTCA GTGAGCTTCT AGGCTCCTTC 
GCTGACAATG AACTGTCACC TGAGTGCCTG GATGGAGCCC AACACTTCCT AAAAGATGAT 
GGTGTGAGCA TCCCTGGGGA GTACACCTCC TTTCTGGCTC CCATCTCCTC CTCCAAGTTA 
TACAATGAGG TCAGAGCCTG TCGAGAAAAG GACCGAGACC CTGAGGCCCA GTTCGAGATG 
CCTTATGTGG TGCGGCTGCA CAACTTCCAC CAGTTGTCTG CGCCCCAGCC CTGTTTCACC 
TTCAGCCATC CCAATAGAGA TCCTATGATT GACAACAACC GCTACTGCAC CTTGGAGTTT 
CCCGTGGAGG TGAATACAGT GCTACATGGC TTTGCAGGCT ACTTTGAGAC TGTGCTTTAT 
CAGGACATCA CCCTAAGTAT CCGTCCAGAA ACTCACTCTC CTGGGATGTT CTCATGGTTT 
CCCATCCTCT TCCCCATCAA GCAGCCCATT ACAGTACGTG AAGGCCAGAC CATCTGTGTG 
CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA 
GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATACA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRMT5 ( XM_537366.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537366.2 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe10131
Clone ID Related Accession (Same CDS sequence)	XM_537366.5 , XM_537366.6
Accession Version	XM_537366.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein arginine N-methyltransferase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537366.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TGGCGGTCGG AGGTGCCGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
AACTGCGTGC CCGAAATAGC TGACACTTTG GGGGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCACTCAGGA ACCTGCTAAG 
AATCGGCCGG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG 
CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATCCGC 
AGGAACTCTG AGGCGGCAAT GTTACAGGAG CTGAATTTTG GGGCATATTT GGGTCTTCCA 
GCTTTCCTGT TGCCCCTAAA TCAGGAAGAT AACACAAACT TGGCCAGGGT TTTGACCAAC 
CACATCCACA CTGGCCACCA CTCGTCCATG TTCTGGATGC GGGTACCCTT GGTGGCTCCA 
GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAAGTA CACATACAGA GGAGTATAGT 
GGAGAGGAGA AGACATGGAT GTGGTGGCAC AACTTCAGGA CCTTGTGTGA CTATAGCAAG 
AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGT 
TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACCA GCATTTTCCT GACCAATAAG 
AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTGA TCTTCCGGCT TCTCAAGTTG 
GAAGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCTTAT 
CTCCAGTACC TGGAATACCT GAGCCAGAAC CGCCCTCCAC CCAATGCCTA TGAACTCTTT 
GCCAAGGGCT ATGAAGACTA TCTGCAGTCT CCACTCCAGC CACTGATGGA CAATCTGGAA 
TCTCAGACTT ATGAAGTGTT TGAAAAGGAC CCTATCAAAT ATTCTCAATA TCAGCAGGCC 
ATCTATAAAT GTCTGTTAGA TCGAGTGCCA GATGAGGAGA AGGATACCAA TATCCAGGTG 
CTAATGGTGC TGGGAGCAGG CCGGGGTCCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG 
CAGGCTGACC GGCGGATAAA GCTGTATGCT GTGGAGAAGA ATCCAAATGC TGTGGTGACG 
CTAGAGAACT GGCAATTTGA AGAATGGGGA AGCCAGGTGA CAGTAGTCTC ATCGGACATG 
CGGGAATGGG TGGCTCCAGA GAAGGCAGAC ATCATCGTCA GTGAGCTTCT AGGCTCCTTC 
GCTGACAATG AACTGTCACC TGAGTGCCTG GATGGAGCCC AACACTTCCT AAAAGATGAT 
GGTGTGAGCA TCCCTGGGGA GTACACCTCC TTTCTGGCTC CCATCTCCTC CTCCAAGTTA 
TACAATGAGG TCAGAGCCTG TCGAGAAAAG GACCGAGACC CTGAGGCCCA GTTCGAGATG 
CCTTATGTGG TGCGGCTGCA CAACTTCCAC CAGTTGTCTG CGCCCCAGCC CTGTTTCACC 
TTCAGCCATC CCAATAGAGA TCCTATGATT GACAACAACC GCTACTGCAC CTTGGAGTTT 
CCCGTGGAGG TGAATACAGT GCTACATGGC TTTGCAGGCT ACTTTGAGAC TGTGCTTTAT 
CAGGACATCA CCCTAAGTAT CCGTCCAGAA ACTCACTCTC CTGGGATGTT CTCATGGTTT 
CCCATCCTCT TCCCCATCAA GCAGCCCATT ACAGTACGTG AAGGCCAGAC CATCTGTGTG 
CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA 
GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATACA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537366.2
CDS	112..2025
Translation	MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFTQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_537366.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537366.6

ORF Insert Sequence:

ATGGCGGCGA TGGCGGTCGG AGGTGCCGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
AACTGCGTGC CCGAAATAGC TGACACTTTG GGGGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCACTCAGGA ACCTGCTAAG 
AATCGGCCGG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGA CTGGAATACG 
CTAATTGTGG GAAAGCTTTC TCCATGGATT CGTCCAGACT CAAAAGTGGA GAAGATCCGC 
AGGAACTCTG AGGCGGCAAT GTTACAGGAG CTGAATTTTG GGGCATATTT GGGTCTTCCA 
GCTTTCCTGT TGCCCCTAAA TCAGGAAGAT AACACAAACT TGGCCAGGGT TTTGACCAAC 
CACATCCACA CTGGCCACCA CTCGTCCATG TTCTGGATGC GGGTACCCTT GGTGGCTCCA 
GAGGACCTGA GAGATGATAT AATTGAGAAT GCACCAAGTA CACATACAGA GGAGTATAGT 
GGAGAGGAGA AGACATGGAT GTGGTGGCAC AACTTCAGGA CCTTGTGTGA CTATAGCAAG 
AGGATTGCAG TGGCTCTTGA AATTGGGGCT GACCTCCCAT CTAATCATGT CATTGATCGT 
TGGCTTGGGG AGCCCATCAA AGCAGCCATT CTCCCCACCA GCATTTTCCT GACCAATAAG 
AAGGGATTTC CTGTTCTTTC TAAGATGCAC CAGAGGCTGA TCTTCCGGCT TCTCAAGTTG 
GAAGTGCAGT TCATCATCAC AGGCACCAAC CACCACTCAG AGAAGGAGTT CTGCTCTTAT 
CTCCAGTACC TGGAATACCT GAGCCAGAAC CGCCCTCCAC CCAATGCCTA TGAACTCTTT 
GCCAAGGGCT ATGAAGACTA TCTGCAGTCT CCACTCCAGC CACTGATGGA CAATCTGGAA 
TCTCAGACTT ATGAAGTGTT TGAAAAGGAC CCTATCAAAT ATTCTCAATA TCAGCAGGCC 
ATCTATAAAT GTCTGTTAGA TCGAGTGCCA GATGAGGAGA AGGATACCAA TATCCAGGTG 
CTAATGGTGC TGGGAGCAGG CCGGGGTCCC CTGGTGAACG CTTCCCTGCG GGCAGCCAAG 
CAGGCTGACC GGCGGATAAA GCTGTATGCT GTGGAGAAGA ATCCAAATGC TGTGGTGACG 
CTAGAGAACT GGCAATTTGA AGAATGGGGA AGCCAGGTGA CAGTAGTCTC ATCGGACATG 
CGGGAATGGG TGGCTCCAGA GAAGGCAGAC ATCATCGTCA GTGAGCTTCT AGGCTCCTTC 
GCTGACAATG AACTGTCACC TGAGTGCCTG GATGGAGCCC AACACTTCCT AAAAGATGAT 
GGTGTGAGCA TCCCTGGGGA GTACACCTCC TTTCTGGCTC CCATCTCCTC CTCCAAGTTA 
TACAATGAGG TCAGAGCCTG TCGAGAAAAG GACCGAGACC CTGAGGCCCA GTTCGAGATG 
CCTTATGTGG TGCGGCTGCA CAACTTCCAC CAGTTGTCTG CGCCCCAGCC CTGTTTCACC 
TTCAGCCATC CCAATAGAGA TCCTATGATT GACAACAACC GCTACTGCAC CTTGGAGTTT 
CCCGTGGAGG TGAATACAGT GCTACATGGC TTTGCAGGCT ACTTTGAGAC TGTGCTTTAT 
CAGGACATCA CCCTAAGTAT CCGTCCAGAA ACTCACTCTC CTGGGATGTT CTCATGGTTT 
CCCATCCTCT TCCCCATCAA GCAGCCCATT ACAGTACGTG AAGGCCAGAC CATCTGTGTG 
CGTTTCTGGC GATGCAGCAA TTCCAAGAAG GTGTGGTATG AGTGGGCTGT GACAGCACCA 
GTCTGTTCTG CTATTCATAA CCCCACAGGC CGCTCATACA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRMT5 ( XM_852436.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_857529.1 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe10132
Clone ID Related Accession (Same CDS sequence)	XM_852436.4 , XM_852436.3
Accession Version	XM_852436.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein arginine N-methyltransferase 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_852436.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTC CGAACTCGGG GACGAAGAGG GACAGACTAG TCATCCCCGA AAGGCAGGGG 
TTTGATTTCC TCTGCATGCC TGTCTTCCAC CCGCGTTTCA AGAGGGAGTT CACTCAGGAA 
CCTGCTAAGA ATCGGCCGGG CCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC 
TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG 
AAGATCCGCA GGAACTCTGA GGCGGCAATG TTACAGGAGC TGAATTTTGG GGCATATTTG 
GGTCTTCCAG CTTTCCTGTT GCCCCTAAAT CAGGAAGATA ACACAAACTT GGCCAGGGTT 
TTGACCAACC ACATCCACAC TGGCCACCAC TCGTCCATGT TCTGGATGCG GGTACCCTTG 
GTGGCTCCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAAGTAC ACATACAGAG 
GAGTATAGTG GAGAGGAGAA GACATGGATG TGGTGGCACA ACTTCAGGAC CTTGTGTGAC 
TATAGCAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC 
ATTGATCGTT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACCAG CATTTTCCTG 
ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTGAT CTTCCGGCTT 
CTCAAGTTGG AAGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC 
TGCTCTTATC TCCAGTACCT GGAATACCTG AGCCAGAACC GCCCTCCACC CAATGCCTAT 
GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCTC CACTCCAGCC ACTGATGGAC 
AATCTGGAAT CTCAGACTTA TGAAGTGTTT GAAAAGGACC CTATCAAATA TTCTCAATAT 
CAGCAGGCCA TCTATAAATG TCTGTTAGAT CGAGTGCCAG ATGAGGAGAA GGATACCAAT 
ATCCAGGTGC TAATGGTGCT GGGAGCAGGC CGGGGTCCCC TGGTGAACGC TTCCCTGCGG 
GCAGCCAAGC AGGCTGACCG GCGGATAAAG CTGTATGCTG TGGAGAAGAA TCCAAATGCT 
GTGGTGACGC TAGAGAACTG GCAATTTGAA GAATGGGGAA GCCAGGTGAC AGTAGTCTCA 
TCGGACATGC GGGAATGGGT GGCTCCAGAG AAGGCAGACA TCATCGTCAG TGAGCTTCTA 
GGCTCCTTCG CTGACAATGA ACTGTCACCT GAGTGCCTGG ATGGAGCCCA ACACTTCCTA 
AAAGATGATG GTGTGAGCAT CCCTGGGGAG TACACCTCCT TTCTGGCTCC CATCTCCTCC 
TCCAAGTTAT ACAATGAGGT CAGAGCCTGT CGAGAAAAGG ACCGAGACCC TGAGGCCCAG 
TTCGAGATGC CTTATGTGGT GCGGCTGCAC AACTTCCACC AGTTGTCTGC GCCCCAGCCC 
TGTTTCACCT TCAGCCATCC CAATAGAGAT CCTATGATTG ACAACAACCG CTACTGCACC 
TTGGAGTTTC CCGTGGAGGT GAATACAGTG CTACATGGCT TTGCAGGCTA CTTTGAGACT 
GTGCTTTATC AGGACATCAC CCTAAGTATC CGTCCAGAAA CTCACTCTCC TGGGATGTTC 
TCATGGTTTC CCATCCTCTT CCCCATCAAG CAGCCCATTA CAGTACGTGA AGGCCAGACC 
ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG 
ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATACAC CATTGGCCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_857529.1
CDS	16..1878
Translation	MRAPNSGTKRDRLVIPERQGFDFLCMPVFHPRFKREFTQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_852436.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_852436.4

ORF Insert Sequence:

ATGCGGGCTC CGAACTCGGG GACGAAGAGG GACAGACTAG TCATCCCCGA AAGGCAGGGG 
TTTGATTTCC TCTGCATGCC TGTCTTCCAC CCGCGTTTCA AGAGGGAGTT CACTCAGGAA 
CCTGCTAAGA ATCGGCCGGG CCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC 
TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG 
AAGATCCGCA GGAACTCTGA GGCGGCAATG TTACAGGAGC TGAATTTTGG GGCATATTTG 
GGTCTTCCAG CTTTCCTGTT GCCCCTAAAT CAGGAAGATA ACACAAACTT GGCCAGGGTT 
TTGACCAACC ACATCCACAC TGGCCACCAC TCGTCCATGT TCTGGATGCG GGTACCCTTG 
GTGGCTCCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAAGTAC ACATACAGAG 
GAGTATAGTG GAGAGGAGAA GACATGGATG TGGTGGCACA ACTTCAGGAC CTTGTGTGAC 
TATAGCAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC 
ATTGATCGTT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACCAG CATTTTCCTG 
ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTGAT CTTCCGGCTT 
CTCAAGTTGG AAGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC 
TGCTCTTATC TCCAGTACCT GGAATACCTG AGCCAGAACC GCCCTCCACC CAATGCCTAT 
GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCTC CACTCCAGCC ACTGATGGAC 
AATCTGGAAT CTCAGACTTA TGAAGTGTTT GAAAAGGACC CTATCAAATA TTCTCAATAT 
CAGCAGGCCA TCTATAAATG TCTGTTAGAT CGAGTGCCAG ATGAGGAGAA GGATACCAAT 
ATCCAGGTGC TAATGGTGCT GGGAGCAGGC CGGGGTCCCC TGGTGAACGC TTCCCTGCGG 
GCAGCCAAGC AGGCTGACCG GCGGATAAAG CTGTATGCTG TGGAGAAGAA TCCAAATGCT 
GTGGTGACGC TAGAGAACTG GCAATTTGAA GAATGGGGAA GCCAGGTGAC AGTAGTCTCA 
TCGGACATGC GGGAATGGGT GGCTCCAGAG AAGGCAGACA TCATCGTCAG TGAGCTTCTA 
GGCTCCTTCG CTGACAATGA ACTGTCACCT GAGTGCCTGG ATGGAGCCCA ACACTTCCTA 
AAAGATGATG GTGTGAGCAT CCCTGGGGAG TACACCTCCT TTCTGGCTCC CATCTCCTCC 
TCCAAGTTAT ACAATGAGGT CAGAGCCTGT CGAGAAAAGG ACCGAGACCC TGAGGCCCAG 
TTCGAGATGC CTTATGTGGT GCGGCTGCAC AACTTCCACC AGTTGTCTGC GCCCCAGCCC 
TGTTTCACCT TCAGCCATCC CAATAGAGAT CCTATGATTG ACAACAACCG CTACTGCACC 
TTGGAGTTTC CCGTGGAGGT GAATACAGTG CTACATGGCT TTGCAGGCTA CTTTGAGACT 
GTGCTTTATC AGGACATCAC CCTAAGTATC CGTCCAGAAA CTCACTCTCC TGGGATGTTC 
TCATGGTTTC CCATCCTCTT CCCCATCAAG CAGCCCATTA CAGTACGTGA AGGCCAGACC 
ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG 
ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATACAC CATTGGCCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRMT5 ( XM_852436.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_857529.1 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe10132
Clone ID Related Accession (Same CDS sequence)	XM_852436.4 , XM_852436.3
Accession Version	XM_852436.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein arginine N-methyltransferase 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_852436.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTC CGAACTCGGG GACGAAGAGG GACAGACTAG TCATCCCCGA AAGGCAGGGG 
TTTGATTTCC TCTGCATGCC TGTCTTCCAC CCGCGTTTCA AGAGGGAGTT CACTCAGGAA 
CCTGCTAAGA ATCGGCCGGG CCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC 
TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG 
AAGATCCGCA GGAACTCTGA GGCGGCAATG TTACAGGAGC TGAATTTTGG GGCATATTTG 
GGTCTTCCAG CTTTCCTGTT GCCCCTAAAT CAGGAAGATA ACACAAACTT GGCCAGGGTT 
TTGACCAACC ACATCCACAC TGGCCACCAC TCGTCCATGT TCTGGATGCG GGTACCCTTG 
GTGGCTCCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAAGTAC ACATACAGAG 
GAGTATAGTG GAGAGGAGAA GACATGGATG TGGTGGCACA ACTTCAGGAC CTTGTGTGAC 
TATAGCAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC 
ATTGATCGTT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACCAG CATTTTCCTG 
ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTGAT CTTCCGGCTT 
CTCAAGTTGG AAGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC 
TGCTCTTATC TCCAGTACCT GGAATACCTG AGCCAGAACC GCCCTCCACC CAATGCCTAT 
GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCTC CACTCCAGCC ACTGATGGAC 
AATCTGGAAT CTCAGACTTA TGAAGTGTTT GAAAAGGACC CTATCAAATA TTCTCAATAT 
CAGCAGGCCA TCTATAAATG TCTGTTAGAT CGAGTGCCAG ATGAGGAGAA GGATACCAAT 
ATCCAGGTGC TAATGGTGCT GGGAGCAGGC CGGGGTCCCC TGGTGAACGC TTCCCTGCGG 
GCAGCCAAGC AGGCTGACCG GCGGATAAAG CTGTATGCTG TGGAGAAGAA TCCAAATGCT 
GTGGTGACGC TAGAGAACTG GCAATTTGAA GAATGGGGAA GCCAGGTGAC AGTAGTCTCA 
TCGGACATGC GGGAATGGGT GGCTCCAGAG AAGGCAGACA TCATCGTCAG TGAGCTTCTA 
GGCTCCTTCG CTGACAATGA ACTGTCACCT GAGTGCCTGG ATGGAGCCCA ACACTTCCTA 
AAAGATGATG GTGTGAGCAT CCCTGGGGAG TACACCTCCT TTCTGGCTCC CATCTCCTCC 
TCCAAGTTAT ACAATGAGGT CAGAGCCTGT CGAGAAAAGG ACCGAGACCC TGAGGCCCAG 
TTCGAGATGC CTTATGTGGT GCGGCTGCAC AACTTCCACC AGTTGTCTGC GCCCCAGCCC 
TGTTTCACCT TCAGCCATCC CAATAGAGAT CCTATGATTG ACAACAACCG CTACTGCACC 
TTGGAGTTTC CCGTGGAGGT GAATACAGTG CTACATGGCT TTGCAGGCTA CTTTGAGACT 
GTGCTTTATC AGGACATCAC CCTAAGTATC CGTCCAGAAA CTCACTCTCC TGGGATGTTC 
TCATGGTTTC CCATCCTCTT CCCCATCAAG CAGCCCATTA CAGTACGTGA AGGCCAGACC 
ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG 
ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATACAC CATTGGCCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_857529.1
CDS	18..1880
Translation	MRAPNSGTKRDRLVIPERQGFDFLCMPVFHPRFKREFTQEPAKNRPGPQTRSDLLLSGRDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_852436.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_852436.3

ORF Insert Sequence:

ATGCGGGCTC CGAACTCGGG GACGAAGAGG GACAGACTAG TCATCCCCGA AAGGCAGGGG 
TTTGATTTCC TCTGCATGCC TGTCTTCCAC CCGCGTTTCA AGAGGGAGTT CACTCAGGAA 
CCTGCTAAGA ATCGGCCGGG CCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGAC 
TGGAATACGC TAATTGTGGG AAAGCTTTCT CCATGGATTC GTCCAGACTC AAAAGTGGAG 
AAGATCCGCA GGAACTCTGA GGCGGCAATG TTACAGGAGC TGAATTTTGG GGCATATTTG 
GGTCTTCCAG CTTTCCTGTT GCCCCTAAAT CAGGAAGATA ACACAAACTT GGCCAGGGTT 
TTGACCAACC ACATCCACAC TGGCCACCAC TCGTCCATGT TCTGGATGCG GGTACCCTTG 
GTGGCTCCAG AGGACCTGAG AGATGATATA ATTGAGAATG CACCAAGTAC ACATACAGAG 
GAGTATAGTG GAGAGGAGAA GACATGGATG TGGTGGCACA ACTTCAGGAC CTTGTGTGAC 
TATAGCAAGA GGATTGCAGT GGCTCTTGAA ATTGGGGCTG ACCTCCCATC TAATCATGTC 
ATTGATCGTT GGCTTGGGGA GCCCATCAAA GCAGCCATTC TCCCCACCAG CATTTTCCTG 
ACCAATAAGA AGGGATTTCC TGTTCTTTCT AAGATGCACC AGAGGCTGAT CTTCCGGCTT 
CTCAAGTTGG AAGTGCAGTT CATCATCACA GGCACCAACC ACCACTCAGA GAAGGAGTTC 
TGCTCTTATC TCCAGTACCT GGAATACCTG AGCCAGAACC GCCCTCCACC CAATGCCTAT 
GAACTCTTTG CCAAGGGCTA TGAAGACTAT CTGCAGTCTC CACTCCAGCC ACTGATGGAC 
AATCTGGAAT CTCAGACTTA TGAAGTGTTT GAAAAGGACC CTATCAAATA TTCTCAATAT 
CAGCAGGCCA TCTATAAATG TCTGTTAGAT CGAGTGCCAG ATGAGGAGAA GGATACCAAT 
ATCCAGGTGC TAATGGTGCT GGGAGCAGGC CGGGGTCCCC TGGTGAACGC TTCCCTGCGG 
GCAGCCAAGC AGGCTGACCG GCGGATAAAG CTGTATGCTG TGGAGAAGAA TCCAAATGCT 
GTGGTGACGC TAGAGAACTG GCAATTTGAA GAATGGGGAA GCCAGGTGAC AGTAGTCTCA 
TCGGACATGC GGGAATGGGT GGCTCCAGAG AAGGCAGACA TCATCGTCAG TGAGCTTCTA 
GGCTCCTTCG CTGACAATGA ACTGTCACCT GAGTGCCTGG ATGGAGCCCA ACACTTCCTA 
AAAGATGATG GTGTGAGCAT CCCTGGGGAG TACACCTCCT TTCTGGCTCC CATCTCCTCC 
TCCAAGTTAT ACAATGAGGT CAGAGCCTGT CGAGAAAAGG ACCGAGACCC TGAGGCCCAG 
TTCGAGATGC CTTATGTGGT GCGGCTGCAC AACTTCCACC AGTTGTCTGC GCCCCAGCCC 
TGTTTCACCT TCAGCCATCC CAATAGAGAT CCTATGATTG ACAACAACCG CTACTGCACC 
TTGGAGTTTC CCGTGGAGGT GAATACAGTG CTACATGGCT TTGCAGGCTA CTTTGAGACT 
GTGCTTTATC AGGACATCAC CCTAAGTATC CGTCCAGAAA CTCACTCTCC TGGGATGTTC 
TCATGGTTTC CCATCCTCTT CCCCATCAAG CAGCCCATTA CAGTACGTGA AGGCCAGACC 
ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT TCCAAGAAGG TGTGGTATGA GTGGGCTGTG 
ACAGCACCAG TCTGTTCTGC TATTCATAAC CCCACAGGCC GCTCATACAC CATTGGCCTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRMT5 ( XM_022421801.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277509.1 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe289980
Clone ID Related Accession (Same CDS sequence)	XM_022421801.1
Accession Version	XM_022421801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1944bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein arginine N-methyltransferase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA TGGCGGTCGG AGGTGCCGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
AACTGCGTGC CCGAAATAGC TGACACTTTG GGGGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCACTCAGGA ACCTGCTAAG 
AATCGGCCGG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGT TTTGTCTTTC 
TTGGATGGGG GGAGTGCAGA CTGGAATACG CTAATTGTGG GAAAGCTTTC TCCATGGATT 
CGTCCAGACT CAAAAGTGGA GAAGATCCGC AGGAACTCTG AGGCGGCAAT GTTACAGGAG 
CTGAATTTTG GGGCATATTT GGGTCTTCCA GCTTTCCTGT TGCCCCTAAA TCAGGAAGAT 
AACACAAACT TGGCCAGGGT TTTGACCAAC CACATCCACA CTGGCCACCA CTCGTCCATG 
TTCTGGATGC GGGTACCCTT GGTGGCTCCA GAGGACCTGA GAGATGATAT AATTGAGAAT 
GCACCAAGTA CACATACAGA GGAGTATAGT GGAGAGGAGA AGACATGGAT GTGGTGGCAC 
AACTTCAGGA CCTTGTGTGA CTATAGCAAG AGGATTGCAG TGGCTCTTGA AATTGGGGCT 
GACCTCCCAT CTAATCATGT CATTGATCGT TGGCTTGGGG AGCCCATCAA AGCAGCCATT 
CTCCCCACCA GCATTTTCCT GACCAATAAG AAGGGATTTC CTGTTCTTTC TAAGATGCAC 
CAGAGGCTGA TCTTCCGGCT TCTCAAGTTG GAAGTGCAGT TCATCATCAC AGGCACCAAC 
CACCACTCAG AGAAGGAGTT CTGCTCTTAT CTCCAGTACC TGGAATACCT GAGCCAGAAC 
CGCCCTCCAC CCAATGCCTA TGAACTCTTT GCCAAGGGCT ATGAAGACTA TCTGCAGTCT 
CCACTCCAGC CACTGATGGA CAATCTGGAA TCTCAGACTT ATGAAGTGTT TGAAAAGGAC 
CCTATCAAAT ATTCTCAATA TCAGCAGGCC ATCTATAAAT GTCTGTTAGA TCGAGTGCCA 
GATGAGGAGA AGGATACCAA TATCCAGGTG CTAATGGTGC TGGGAGCAGG CCGGGGTCCC 
CTGGTGAACG CTTCCCTGCG GGCAGCCAAG CAGGCTGACC GGCGGATAAA GCTGTATGCT 
GTGGAGAAGA ATCCAAATGC TGTGGTGACG CTAGAGAACT GGCAATTTGA AGAATGGGGA 
AGCCAGGTGA CAGTAGTCTC ATCGGACATG CGGGAATGGG TGGCTCCAGA GAAGGCAGAC 
ATCATCGTCA GTGAGCTTCT AGGCTCCTTC GCTGACAATG AACTGTCACC TGAGTGCCTG 
GATGGAGCCC AACACTTCCT AAAAGATGAT GGTGTGAGCA TCCCTGGGGA GTACACCTCC 
TTTCTGGCTC CCATCTCCTC CTCCAAGTTA TACAATGAGG TCAGAGCCTG TCGAGAAAAG 
GACCGAGACC CTGAGGCCCA GTTCGAGATG CCTTATGTGG TGCGGCTGCA CAACTTCCAC 
CAGTTGTCTG CGCCCCAGCC CTGTTTCACC TTCAGCCATC CCAATAGAGA TCCTATGATT 
GACAACAACC GCTACTGCAC CTTGGAGTTT CCCGTGGAGG TGAATACAGT GCTACATGGC 
TTTGCAGGCT ACTTTGAGAC TGTGCTTTAT CAGGACATCA CCCTAAGTAT CCGTCCAGAA 
ACTCACTCTC CTGGGATGTT CTCATGGTTT CCCATCCTCT TCCCCATCAA GCAGCCCATT 
ACAGTACGTG AAGGCCAGAC CATCTGTGTG CGTTTCTGGC GATGCAGCAA TTCCAAGAAG 
GTGTGGTATG AGTGGGCTGT GACAGCACCA GTCTGTTCTG CTATTCATAA CCCCACAGGC 
CGCTCATACA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277509.1
CDS	111..2054
Translation	MAAMAVGGAGGSRVSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFTQEPAKNRPGPQTRSDLLLSGRVLSFLDGGSADWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_022421801.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421801.1

ORF Insert Sequence:

ATGGCGGCGA TGGCGGTCGG AGGTGCCGGT GGGAGCCGCG TGTCCAGCGG GAGGGACCTG 
AACTGCGTGC CCGAAATAGC TGACACTTTG GGGGCTGTGG CCAAGCAGGG GTTTGATTTC 
CTCTGCATGC CTGTCTTCCA CCCGCGTTTC AAGAGGGAGT TCACTCAGGA ACCTGCTAAG 
AATCGGCCGG GCCCCCAGAC ACGATCAGAC CTACTGCTGT CAGGAAGGGT TTTGTCTTTC 
TTGGATGGGG GGAGTGCAGA CTGGAATACG CTAATTGTGG GAAAGCTTTC TCCATGGATT 
CGTCCAGACT CAAAAGTGGA GAAGATCCGC AGGAACTCTG AGGCGGCAAT GTTACAGGAG 
CTGAATTTTG GGGCATATTT GGGTCTTCCA GCTTTCCTGT TGCCCCTAAA TCAGGAAGAT 
AACACAAACT TGGCCAGGGT TTTGACCAAC CACATCCACA CTGGCCACCA CTCGTCCATG 
TTCTGGATGC GGGTACCCTT GGTGGCTCCA GAGGACCTGA GAGATGATAT AATTGAGAAT 
GCACCAAGTA CACATACAGA GGAGTATAGT GGAGAGGAGA AGACATGGAT GTGGTGGCAC 
AACTTCAGGA CCTTGTGTGA CTATAGCAAG AGGATTGCAG TGGCTCTTGA AATTGGGGCT 
GACCTCCCAT CTAATCATGT CATTGATCGT TGGCTTGGGG AGCCCATCAA AGCAGCCATT 
CTCCCCACCA GCATTTTCCT GACCAATAAG AAGGGATTTC CTGTTCTTTC TAAGATGCAC 
CAGAGGCTGA TCTTCCGGCT TCTCAAGTTG GAAGTGCAGT TCATCATCAC AGGCACCAAC 
CACCACTCAG AGAAGGAGTT CTGCTCTTAT CTCCAGTACC TGGAATACCT GAGCCAGAAC 
CGCCCTCCAC CCAATGCCTA TGAACTCTTT GCCAAGGGCT ATGAAGACTA TCTGCAGTCT 
CCACTCCAGC CACTGATGGA CAATCTGGAA TCTCAGACTT ATGAAGTGTT TGAAAAGGAC 
CCTATCAAAT ATTCTCAATA TCAGCAGGCC ATCTATAAAT GTCTGTTAGA TCGAGTGCCA 
GATGAGGAGA AGGATACCAA TATCCAGGTG CTAATGGTGC TGGGAGCAGG CCGGGGTCCC 
CTGGTGAACG CTTCCCTGCG GGCAGCCAAG CAGGCTGACC GGCGGATAAA GCTGTATGCT 
GTGGAGAAGA ATCCAAATGC TGTGGTGACG CTAGAGAACT GGCAATTTGA AGAATGGGGA 
AGCCAGGTGA CAGTAGTCTC ATCGGACATG CGGGAATGGG TGGCTCCAGA GAAGGCAGAC 
ATCATCGTCA GTGAGCTTCT AGGCTCCTTC GCTGACAATG AACTGTCACC TGAGTGCCTG 
GATGGAGCCC AACACTTCCT AAAAGATGAT GGTGTGAGCA TCCCTGGGGA GTACACCTCC 
TTTCTGGCTC CCATCTCCTC CTCCAAGTTA TACAATGAGG TCAGAGCCTG TCGAGAAAAG 
GACCGAGACC CTGAGGCCCA GTTCGAGATG CCTTATGTGG TGCGGCTGCA CAACTTCCAC 
CAGTTGTCTG CGCCCCAGCC CTGTTTCACC TTCAGCCATC CCAATAGAGA TCCTATGATT 
GACAACAACC GCTACTGCAC CTTGGAGTTT CCCGTGGAGG TGAATACAGT GCTACATGGC 
TTTGCAGGCT ACTTTGAGAC TGTGCTTTAT CAGGACATCA CCCTAAGTAT CCGTCCAGAA 
ACTCACTCTC CTGGGATGTT CTCATGGTTT CCCATCCTCT TCCCCATCAA GCAGCCCATT 
ACAGTACGTG AAGGCCAGAC CATCTGTGTG CGTTTCTGGC GATGCAGCAA TTCCAAGAAG 
GTGTGGTATG AGTGGGCTGT GACAGCACCA GTCTGTTCTG CTATTCATAA CCCCACAGGC 
CGCTCATACA CCATTGGCCT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRMT5 ( XM_022421802.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022277510.1 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.

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CloneID	OCe289981
Clone ID Related Accession (Same CDS sequence)	XM_022421802.1
Accession Version	XM_022421802.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	protein arginine N-methyltransferase 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006590.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTC CGAACTCGGG GACGAAGAGG GACAGACTAG TCATCCCCGA AAGGCAGGGG 
TTTGATTTCC TCTGCATGCC TGTCTTCCAC CCGCGTTTCA AGAGGGAGTT CACTCAGGAA 
CCTGCTAAGA ATCGGCCGGG CCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGTT 
TTGTCTTTCT TGGATGGGGG GAGTGCAGAC TGGAATACGC TAATTGTGGG AAAGCTTTCT 
CCATGGATTC GTCCAGACTC AAAAGTGGAG AAGATCCGCA GGAACTCTGA GGCGGCAATG 
TTACAGGAGC TGAATTTTGG GGCATATTTG GGTCTTCCAG CTTTCCTGTT GCCCCTAAAT 
CAGGAAGATA ACACAAACTT GGCCAGGGTT TTGACCAACC ACATCCACAC TGGCCACCAC 
TCGTCCATGT TCTGGATGCG GGTACCCTTG GTGGCTCCAG AGGACCTGAG AGATGATATA 
ATTGAGAATG CACCAAGTAC ACATACAGAG GAGTATAGTG GAGAGGAGAA GACATGGATG 
TGGTGGCACA ACTTCAGGAC CTTGTGTGAC TATAGCAAGA GGATTGCAGT GGCTCTTGAA 
ATTGGGGCTG ACCTCCCATC TAATCATGTC ATTGATCGTT GGCTTGGGGA GCCCATCAAA 
GCAGCCATTC TCCCCACCAG CATTTTCCTG ACCAATAAGA AGGGATTTCC TGTTCTTTCT 
AAGATGCACC AGAGGCTGAT CTTCCGGCTT CTCAAGTTGG AAGTGCAGTT CATCATCACA 
GGCACCAACC ACCACTCAGA GAAGGAGTTC TGCTCTTATC TCCAGTACCT GGAATACCTG 
AGCCAGAACC GCCCTCCACC CAATGCCTAT GAACTCTTTG CCAAGGGCTA TGAAGACTAT 
CTGCAGTCTC CACTCCAGCC ACTGATGGAC AATCTGGAAT CTCAGACTTA TGAAGTGTTT 
GAAAAGGACC CTATCAAATA TTCTCAATAT CAGCAGGCCA TCTATAAATG TCTGTTAGAT 
CGAGTGCCAG ATGAGGAGAA GGATACCAAT ATCCAGGTGC TAATGGTGCT GGGAGCAGGC 
CGGGGTCCCC TGGTGAACGC TTCCCTGCGG GCAGCCAAGC AGGCTGACCG GCGGATAAAG 
CTGTATGCTG TGGAGAAGAA TCCAAATGCT GTGGTGACGC TAGAGAACTG GCAATTTGAA 
GAATGGGGAA GCCAGGTGAC AGTAGTCTCA TCGGACATGC GGGAATGGGT GGCTCCAGAG 
AAGGCAGACA TCATCGTCAG TGAGCTTCTA GGCTCCTTCG CTGACAATGA ACTGTCACCT 
GAGTGCCTGG ATGGAGCCCA ACACTTCCTA AAAGATGATG GTGTGAGCAT CCCTGGGGAG 
TACACCTCCT TTCTGGCTCC CATCTCCTCC TCCAAGTTAT ACAATGAGGT CAGAGCCTGT 
CGAGAAAAGG ACCGAGACCC TGAGGCCCAG TTCGAGATGC CTTATGTGGT GCGGCTGCAC 
AACTTCCACC AGTTGTCTGC GCCCCAGCCC TGTTTCACCT TCAGCCATCC CAATAGAGAT 
CCTATGATTG ACAACAACCG CTACTGCACC TTGGAGTTTC CCGTGGAGGT GAATACAGTG 
CTACATGGCT TTGCAGGCTA CTTTGAGACT GTGCTTTATC AGGACATCAC CCTAAGTATC 
CGTCCAGAAA CTCACTCTCC TGGGATGTTC TCATGGTTTC CCATCCTCTT CCCCATCAAG 
CAGCCCATTA CAGTACGTGA AGGCCAGACC ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT 
TCCAAGAAGG TGTGGTATGA GTGGGCTGTG ACAGCACCAG TCTGTTCTGC TATTCATAAC 
CCCACAGGCC GCTCATACAC CATTGGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022277510.1
CDS	16..1908
Translation	MRAPNSGTKRDRLVIPERQGFDFLCMPVFHPRFKREFTQEPAKNRPGPQTRSDLLLSGRVLSFLDGGSADWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_022421802.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421802.1

ORF Insert Sequence:

ATGCGGGCTC CGAACTCGGG GACGAAGAGG GACAGACTAG TCATCCCCGA AAGGCAGGGG 
TTTGATTTCC TCTGCATGCC TGTCTTCCAC CCGCGTTTCA AGAGGGAGTT CACTCAGGAA 
CCTGCTAAGA ATCGGCCGGG CCCCCAGACA CGATCAGACC TACTGCTGTC AGGAAGGGTT 
TTGTCTTTCT TGGATGGGGG GAGTGCAGAC TGGAATACGC TAATTGTGGG AAAGCTTTCT 
CCATGGATTC GTCCAGACTC AAAAGTGGAG AAGATCCGCA GGAACTCTGA GGCGGCAATG 
TTACAGGAGC TGAATTTTGG GGCATATTTG GGTCTTCCAG CTTTCCTGTT GCCCCTAAAT 
CAGGAAGATA ACACAAACTT GGCCAGGGTT TTGACCAACC ACATCCACAC TGGCCACCAC 
TCGTCCATGT TCTGGATGCG GGTACCCTTG GTGGCTCCAG AGGACCTGAG AGATGATATA 
ATTGAGAATG CACCAAGTAC ACATACAGAG GAGTATAGTG GAGAGGAGAA GACATGGATG 
TGGTGGCACA ACTTCAGGAC CTTGTGTGAC TATAGCAAGA GGATTGCAGT GGCTCTTGAA 
ATTGGGGCTG ACCTCCCATC TAATCATGTC ATTGATCGTT GGCTTGGGGA GCCCATCAAA 
GCAGCCATTC TCCCCACCAG CATTTTCCTG ACCAATAAGA AGGGATTTCC TGTTCTTTCT 
AAGATGCACC AGAGGCTGAT CTTCCGGCTT CTCAAGTTGG AAGTGCAGTT CATCATCACA 
GGCACCAACC ACCACTCAGA GAAGGAGTTC TGCTCTTATC TCCAGTACCT GGAATACCTG 
AGCCAGAACC GCCCTCCACC CAATGCCTAT GAACTCTTTG CCAAGGGCTA TGAAGACTAT 
CTGCAGTCTC CACTCCAGCC ACTGATGGAC AATCTGGAAT CTCAGACTTA TGAAGTGTTT 
GAAAAGGACC CTATCAAATA TTCTCAATAT CAGCAGGCCA TCTATAAATG TCTGTTAGAT 
CGAGTGCCAG ATGAGGAGAA GGATACCAAT ATCCAGGTGC TAATGGTGCT GGGAGCAGGC 
CGGGGTCCCC TGGTGAACGC TTCCCTGCGG GCAGCCAAGC AGGCTGACCG GCGGATAAAG 
CTGTATGCTG TGGAGAAGAA TCCAAATGCT GTGGTGACGC TAGAGAACTG GCAATTTGAA 
GAATGGGGAA GCCAGGTGAC AGTAGTCTCA TCGGACATGC GGGAATGGGT GGCTCCAGAG 
AAGGCAGACA TCATCGTCAG TGAGCTTCTA GGCTCCTTCG CTGACAATGA ACTGTCACCT 
GAGTGCCTGG ATGGAGCCCA ACACTTCCTA AAAGATGATG GTGTGAGCAT CCCTGGGGAG 
TACACCTCCT TTCTGGCTCC CATCTCCTCC TCCAAGTTAT ACAATGAGGT CAGAGCCTGT 
CGAGAAAAGG ACCGAGACCC TGAGGCCCAG TTCGAGATGC CTTATGTGGT GCGGCTGCAC 
AACTTCCACC AGTTGTCTGC GCCCCAGCCC TGTTTCACCT TCAGCCATCC CAATAGAGAT 
CCTATGATTG ACAACAACCG CTACTGCACC TTGGAGTTTC CCGTGGAGGT GAATACAGTG 
CTACATGGCT TTGCAGGCTA CTTTGAGACT GTGCTTTATC AGGACATCAC CCTAAGTATC 
CGTCCAGAAA CTCACTCTCC TGGGATGTTC TCATGGTTTC CCATCCTCTT CCCCATCAAG 
CAGCCCATTA CAGTACGTGA AGGCCAGACC ATCTGTGTGC GTTTCTGGCG ATGCAGCAAT 
TCCAAGAAGG TGTGGTATGA GTGGGCTGTG ACAGCACCAG TCTGTTCTGC TATTCATAAC 
CCCACAGGCC GCTCATACAC CATTGGCCTC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRMT5 ( XM_014115755.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013971230.1 Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X2, mRNA.

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CloneID	OCe37813
Clone ID Related Accession (Same CDS sequence)	XM_014115755.1
Accession Version	XM_014115755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein arginine N-methyltransferase 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726071.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACAGG AGCTGAATTT TGGGGCATAT TTGGGTCTTC CAGCTTTCCT GTTGCCCCTA 
AATCAGGAAG ATAACACAAA CTTGGCCAGG GTTTTGACCA ACCACATCCA CACTGGCCAC 
CACTCGTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCTC CAGAGGACCT GAGAGATGAT 
ATAATTGAGA ATGCACCAAG TACACATACA GAGGAGTATA GTGGAGAGGA GAAGACATGG 
ATGTGGTGGC ACAACTTCAG GACCTTGTGT GACTATAGCA AGAGGATTGC AGTGGCTCTT 
GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GTTGGCTTGG GGAGCCCATC 
AAAGCAGCCA TTCTCCCCAC CAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT 
TCTAAGATGC ACCAGAGGCT GATCTTCCGG CTTCTCAAGT TGGAAGTGCA GTTCATCATC 
ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCTT ATCTCCAGTA CCTGGAATAC 
CTGAGCCAGA ACCGCCCTCC ACCCAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC 
TATCTGCAGT CTCCACTCCA GCCACTGATG GACAATCTGG AATCTCAGAC TTATGAAGTG 
TTTGAAAAGG ACCCTATCAA ATATTCTCAA TATCAGCAGG CCATCTATAA ATGTCTGTTA 
GATCGAGTGC CAGATGAGGA GAAGGATACC AATATCCAGG TGCTAATGGT GCTGGGAGCA 
GGCCGGGGTC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCTGA CCGGCGGATA 
AAGCTGTATG CTGTGGAGAA GAATCCAAAT GCTGTGGTGA CGCTAGAGAA CTGGCAATTT 
GAAGAATGGG GAAGCCAGGT GACAGTAGTC TCATCGGACA TGCGGGAATG GGTGGCTCCA 
GAGAAGGCAG ACATCATCGT CAGTGAGCTT CTAGGCTCCT TCGCTGACAA TGAACTGTCA 
CCTGAGTGCC TGGATGGAGC CCAACACTTC CTAAAAGATG ATGGTGTGAG CATCCCTGGG 
GAGTACACCT CCTTTCTGGC TCCCATCTCC TCCTCCAAGT TATACAATGA GGTCAGAGCC 
TGTCGAGAAA AGGACCGAGA CCCTGAGGCC CAGTTCGAGA TGCCTTATGT GGTGCGGCTG 
CACAACTTCC ACCAGTTGTC TGCGCCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAATAGA 
GATCCTATGA TTGACAACAA CCGCTACTGC ACCTTGGAGT TTCCCGTGGA GGTGAATACA 
GTGCTACATG GCTTTGCAGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACCCTAAGT 
ATCCGTCCAG AAACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCCATC 
AAGCAGCCCA TTACAGTACG TGAAGGCCAG ACCATCTGTG TGCGTTTCTG GCGATGCAGC 
AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT 
AACCCCACAG GCCGCTCATA CACCATTGGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013971230.1
CDS	178..1773
Translation	MLQELNFGAYLGLPAFLLPLNQEDNTNLARVLTNHIHTGHHSSMFWMRVPLVAPEDLRDDIIENAPSTHTEEYSGEEKTWMWWHNFRTLCDYSKRIAVALEIGADLPSNHVIDRWLGEPIKAAILPTSIFLTNKKGFPVLSKMHQRLIFRLLKLEVQFIITGTNHHSEKEFCSYLQYLEYLSQNRPPPNAYELFAKGYEDYLQSPLQPLMDNLESQTYEVFEKDPIKYSQYQQAIYKCLLDRVPDEEKDTNIQVLMVLGAGRGPLVNASLRAAKQADRRIKLYAVEKNPNAVVTLENWQFEEWGSQVTVVSSDMREWVAPEKADIIVSELLGSFADNELSPECLDGAQHFLKDDGVSIPGEYTSFLAPISSSKLYNEVRACREKDRDPEAQFEMPYVVRLHNFHQLSAPQPCFTFSHPNRDPMIDNNRYCTLEFPVEVNTVLHGFAGYFETVLYQDITLSIRPETHSPGMFSWFPILFPIKQPITVREGQTICVRFWRCSNSKKVWYEWAVTAPVCSAIHNPTGRSYTIGL

Target ORF information:

RefSeq Version	XM_014115755.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris protein arginine methyltransferase 5 (PRMT5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115755.1

ORF Insert Sequence:

ATGTTACAGG AGCTGAATTT TGGGGCATAT TTGGGTCTTC CAGCTTTCCT GTTGCCCCTA 
AATCAGGAAG ATAACACAAA CTTGGCCAGG GTTTTGACCA ACCACATCCA CACTGGCCAC 
CACTCGTCCA TGTTCTGGAT GCGGGTACCC TTGGTGGCTC CAGAGGACCT GAGAGATGAT 
ATAATTGAGA ATGCACCAAG TACACATACA GAGGAGTATA GTGGAGAGGA GAAGACATGG 
ATGTGGTGGC ACAACTTCAG GACCTTGTGT GACTATAGCA AGAGGATTGC AGTGGCTCTT 
GAAATTGGGG CTGACCTCCC ATCTAATCAT GTCATTGATC GTTGGCTTGG GGAGCCCATC 
AAAGCAGCCA TTCTCCCCAC CAGCATTTTC CTGACCAATA AGAAGGGATT TCCTGTTCTT 
TCTAAGATGC ACCAGAGGCT GATCTTCCGG CTTCTCAAGT TGGAAGTGCA GTTCATCATC 
ACAGGCACCA ACCACCACTC AGAGAAGGAG TTCTGCTCTT ATCTCCAGTA CCTGGAATAC 
CTGAGCCAGA ACCGCCCTCC ACCCAATGCC TATGAACTCT TTGCCAAGGG CTATGAAGAC 
TATCTGCAGT CTCCACTCCA GCCACTGATG GACAATCTGG AATCTCAGAC TTATGAAGTG 
TTTGAAAAGG ACCCTATCAA ATATTCTCAA TATCAGCAGG CCATCTATAA ATGTCTGTTA 
GATCGAGTGC CAGATGAGGA GAAGGATACC AATATCCAGG TGCTAATGGT GCTGGGAGCA 
GGCCGGGGTC CCCTGGTGAA CGCTTCCCTG CGGGCAGCCA AGCAGGCTGA CCGGCGGATA 
AAGCTGTATG CTGTGGAGAA GAATCCAAAT GCTGTGGTGA CGCTAGAGAA CTGGCAATTT 
GAAGAATGGG GAAGCCAGGT GACAGTAGTC TCATCGGACA TGCGGGAATG GGTGGCTCCA 
GAGAAGGCAG ACATCATCGT CAGTGAGCTT CTAGGCTCCT TCGCTGACAA TGAACTGTCA 
CCTGAGTGCC TGGATGGAGC CCAACACTTC CTAAAAGATG ATGGTGTGAG CATCCCTGGG 
GAGTACACCT CCTTTCTGGC TCCCATCTCC TCCTCCAAGT TATACAATGA GGTCAGAGCC 
TGTCGAGAAA AGGACCGAGA CCCTGAGGCC CAGTTCGAGA TGCCTTATGT GGTGCGGCTG 
CACAACTTCC ACCAGTTGTC TGCGCCCCAG CCCTGTTTCA CCTTCAGCCA TCCCAATAGA 
GATCCTATGA TTGACAACAA CCGCTACTGC ACCTTGGAGT TTCCCGTGGA GGTGAATACA 
GTGCTACATG GCTTTGCAGG CTACTTTGAG ACTGTGCTTT ATCAGGACAT CACCCTAAGT 
ATCCGTCCAG AAACTCACTC TCCTGGGATG TTCTCATGGT TTCCCATCCT CTTCCCCATC 
AAGCAGCCCA TTACAGTACG TGAAGGCCAG ACCATCTGTG TGCGTTTCTG GCGATGCAGC 
AATTCCAAGA AGGTGTGGTA TGAGTGGGCT GTGACAGCAC CAGTCTGTTC TGCTATTCAT 
AACCCCACAG GCCGCTCATA CACCATTGGC CTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PRMT5 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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