RFWD2 cDNA ORF clone, Canis lupus familiaris(dog)

The following RFWD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe289540 XM_014115170.2
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OCe09217 XM_537181.6
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe289537 XM_022421014.1
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe289538 XM_022421015.1
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe289539 XM_022421016.1
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $342.30
$489.00
OCe37445 XM_014115169.1
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe37446 XM_014115170.1
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe37447 XM_014115171.2
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe37448 XM_014115172.2
Latest version!
Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OCe09217 XM_537181.5 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe37448 XM_014115172.1 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OCe37447 XM_014115171.1 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe289540
    Clone ID Related Accession (Same CDS sequence) XM_014115170.2
    Accession Version XM_014115170.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATTGAGG AAGCATACAT GACGAAATGT GGCCACAGCT TTTGCTACAA GTGTATTCAT 
    CAGAGTTTGG AGGATAATAA TAGATGTCCA AAGTGTAACT ATGTTGTGGA TAATATTGAC
    CATCTGTATC CTAATTTCTT GGTGAATGAG CTTATTCTCA AACAGAAGCA AAGATTTGAG
    GAAAAGAGGT TCAAATTAGA CCACTCAAAT GGCCACAGGT GGCAAATATT ACAGGATTTG
    TTGGGAACTG ACCAAGATAA CCTTGATTTG GCCAATGTCA ACCTCATGTT GGAATTACTA
    GTGCAGAAGA AGAAACAACT GGAAGCAGAA TCACATGCAG CCCAACTACA GATCCTTATG
    GAATTCCTCA AGGTTGCAAG AAGAAATAAG AGAGAGCAAC TGGAACAGAT CCAGAAGGAG
    CTGAGTGTTT TGGAAGAGGA TATTAAAAGA GTGGAAGAAA TGAGTGGCTT GTATTCTCCT
    GTCAGTGAGG ATAGCACGGT GCCTCAATTT GAAGCTCCTT CTCCATCACA CAGTAATATT
    ATTGATTCCA CAGAATACAG CCAACCTCCT GGTTTCAGTG GCAGTTCTCA GACAAAGAAG
    CAGCCTTGGT ATAACAGCAC ATTAGCATCA CGAAGAAAAC GACTCACTGC TCATTTTGAA
    GACTTAGAGC AATGTTACTT TTCTACAAGG ATGTCTCGTA TTTCAGATGA TAGTCGAACT
    GCAAGCCAGT TGGATGAATT TCAGGAATGC TTGTCCAAGT TTACTCGGTA TAATTCAGTA
    CGACCTTTGG CTACATTGTC ATATGCTAGT GATCTCTATA ATGGTTCCAG CATAGTCTCT
    AGTATTGAAT TTGACCGAGA TTGTGACTAT TTTGCAATTG CTGGGGTTAC AAAGAAGATT
    AAAGTCTATG AATATGGCAC AGTCATTCAG GATGCGGTGG ATATTCATTA CCCTGAAAAT
    GAAATGACCT GCAATTCCAA AATCAGCTGT ATCAGTTGGA GTAGTTACCA TAAGAATCTG
    TTAGCCAGCA GTGATTATGA AGGCACTGTC ATCCTATGGG ATGGATTCAC AGGACAGAGG
    TCAAAGGTCT ATCAGGAACA TGAGAAAAGG TGTTGGAGTG TTGACTTTAA CTTAATGGAT
    CCTAAACTCT TGGCATCGGG TTCTGATGAT GCAAAAGTGA AGCTATGGTC TACCAACCTA
    GACAACTCAG TGGCAAGCAT TGAGGCGAAG GCTAATGTAT GCTGTGTTAA ATTCAGCCCT
    TCTTCTAGAT ACCATTTGGC TTTTGGCTGT GCAGATCACT GTGTCCACTA CTATGATCTT
    CGTAACACTA AACAGCCAAT CATGGTATTC AAGGGACACC GAAAAGCAGT CTCTTATGCA
    AAGTTTGTGA GTGGTGAGGA AATCGTCTCT GCCTCAACAG ACAGCCAGCT AAAGCTGTGG
    AATGTAGGGA AACCATACTG TCTACGTTCC TTCAAGGGTC ACATCAATGA AAAAAACTTT
    GTAGGCCTCG CTTCCAATGG CGATTATATA GCTTGTGGAA GTGAGAACAA CTCTCTTTAC
    TTATACTATA AAGGACTTTC TAAGACTTTG CTAACTTTTA AGTTTGATAC GGTCAAAAGT
    GTTCTGGATA AAGACCGAAA AGAAGATGAT ACAAATGAAT TTGTTAGTGC CGTGTGCTGG
    AGGGCATTGC CAGATGGGGA GTCCAATGTG CTGATTGCTG CTAACAGTCA GGGTACAATT
    AAGGTGCTGG AGTTGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970645.2
    CDS143..1903
    Translation

    Target ORF information:

    RefSeq Version XM_014115170.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115170.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATTGAGG AAGCATACAT GACGAAATGT GGCCACAGCT TTTGCTACAA GTGTATTCAT 
    CAGAGTTTGG AGGATAATAA TAGATGTCCA AAGTGTAACT ATGTTGTGGA TAATATTGAC
    CATCTGTATC CTAATTTCTT GGTGAATGAG CTTATTCTCA AACAGAAGCA AAGATTTGAG
    GAAAAGAGGT TCAAATTAGA CCACTCAAAT GGCCACAGGT GGCAAATATT ACAGGATTTG
    TTGGGAACTG ACCAAGATAA CCTTGATTTG GCCAATGTCA ACCTCATGTT GGAATTACTA
    GTGCAGAAGA AGAAACAACT GGAAGCAGAA TCACATGCAG CCCAACTACA GATCCTTATG
    GAATTCCTCA AGGTTGCAAG AAGAAATAAG AGAGAGCAAC TGGAACAGAT CCAGAAGGAG
    CTGAGTGTTT TGGAAGAGGA TATTAAAAGA GTGGAAGAAA TGAGTGGCTT GTATTCTCCT
    GTCAGTGAGG ATAGCACGGT GCCTCAATTT GAAGCTCCTT CTCCATCACA CAGTAATATT
    ATTGATTCCA CAGAATACAG CCAACCTCCT GGTTTCAGTG GCAGTTCTCA GACAAAGAAG
    CAGCCTTGGT ATAACAGCAC ATTAGCATCA CGAAGAAAAC GACTCACTGC TCATTTTGAA
    GACTTAGAGC AATGTTACTT TTCTACAAGG ATGTCTCGTA TTTCAGATGA TAGTCGAACT
    GCAAGCCAGT TGGATGAATT TCAGGAATGC TTGTCCAAGT TTACTCGGTA TAATTCAGTA
    CGACCTTTGG CTACATTGTC ATATGCTAGT GATCTCTATA ATGGTTCCAG CATAGTCTCT
    AGTATTGAAT TTGACCGAGA TTGTGACTAT TTTGCAATTG CTGGGGTTAC AAAGAAGATT
    AAAGTCTATG AATATGGCAC AGTCATTCAG GATGCGGTGG ATATTCATTA CCCTGAAAAT
    GAAATGACCT GCAATTCCAA AATCAGCTGT ATCAGTTGGA GTAGTTACCA TAAGAATCTG
    TTAGCCAGCA GTGATTATGA AGGCACTGTC ATCCTATGGG ATGGATTCAC AGGACAGAGG
    TCAAAGGTCT ATCAGGAACA TGAGAAAAGG TGTTGGAGTG TTGACTTTAA CTTAATGGAT
    CCTAAACTCT TGGCATCGGG TTCTGATGAT GCAAAAGTGA AGCTATGGTC TACCAACCTA
    GACAACTCAG TGGCAAGCAT TGAGGCGAAG GCTAATGTAT GCTGTGTTAA ATTCAGCCCT
    TCTTCTAGAT ACCATTTGGC TTTTGGCTGT GCAGATCACT GTGTCCACTA CTATGATCTT
    CGTAACACTA AACAGCCAAT CATGGTATTC AAGGGACACC GAAAAGCAGT CTCTTATGCA
    AAGTTTGTGA GTGGTGAGGA AATCGTCTCT GCCTCAACAG ACAGCCAGCT AAAGCTGTGG
    AATGTAGGGA AACCATACTG TCTACGTTCC TTCAAGGGTC ACATCAATGA AAAAAACTTT
    GTAGGCCTCG CTTCCAATGG CGATTATATA GCTTGTGGAA GTGAGAACAA CTCTCTTTAC
    TTATACTATA AAGGACTTTC TAAGACTTTG CTAACTTTTA AGTTTGATAC GGTCAAAAGT
    GTTCTGGATA AAGACCGAAA AGAAGATGAT ACAAATGAAT TTGTTAGTGC CGTGTGCTGG
    AGGGCATTGC CAGATGGGGA GTCCAATGTG CTGATTGCTG CTAACAGTCA GGGTACAATT
    AAGGTGCTGG AGTTGGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09217
    Clone ID Related Accession (Same CDS sequence) XM_537181.5 , XM_537181.6
    Accession Version XM_537181.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537181.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA
    CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA
    ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT
    TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT
    GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT
    ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG
    TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT
    AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT
    GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG
    GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG
    AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT
    GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG
    AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA
    TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC
    TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC
    CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA
    GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT
    CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA
    AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT
    AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA
    TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT
    GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537181.2
    CDS99..2300
    Translation

    Target ORF information:

    RefSeq Version XM_537181.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537181.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA
    CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA
    ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT
    TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT
    GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT
    ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG
    TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT
    AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT
    GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG
    GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG
    AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT
    GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG
    AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA
    TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC
    TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC
    CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA
    GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT
    CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA
    AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT
    AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA
    TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT
    GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe09217
    Clone ID Related Accession (Same CDS sequence) XM_537181.5 , XM_537181.6
    Accession Version XM_537181.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537181.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA
    CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA
    ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT
    TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT
    GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT
    ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG
    TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT
    AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT
    GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG
    GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG
    AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT
    GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG
    AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA
    TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC
    TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC
    CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA
    GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT
    CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA
    AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT
    AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA
    TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT
    GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_537181.2
    CDS82..2283
    Translation

    Target ORF information:

    RefSeq Version XM_537181.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_537181.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA
    CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA
    ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT
    TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT
    GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT
    ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG
    TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT
    AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT
    GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG
    GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG
    AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT
    GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG
    AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA
    TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC
    TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC
    CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA
    GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT
    CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA
    AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT
    AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA
    TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT
    GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289537
    Clone ID Related Accession (Same CDS sequence) XM_022421014.1
    Accession Version XM_022421014.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGGAA
    ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT
    TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT
    GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT
    ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG
    TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT
    AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT
    GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG
    GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG
    AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT
    GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG
    AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA
    TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC
    TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC
    CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA
    GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT
    CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA
    AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT
    AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA
    TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT
    GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276722.1
    CDS83..2224
    Translation

    Target ORF information:

    RefSeq Version XM_022421014.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGGAA
    ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT
    TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT
    GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA
    CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT
    ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG
    TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT
    AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT
    GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG
    GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG
    AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG
    GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT
    GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG
    AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA
    TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC
    TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC
    CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA
    GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT
    CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA
    AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT
    AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA
    TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT
    GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289538
    Clone ID Related Accession (Same CDS sequence) XM_022421015.1
    Accession Version XM_022421015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA
    CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG
    GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG
    ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGGAAAT GAGTGGCTTG
    TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC
    AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG
    ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT
    CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT
    AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT
    AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC
    ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA
    AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC
    CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT
    AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA
    GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC
    TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT
    ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA
    TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC
    TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC
    TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA
    AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA
    AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC
    TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG
    GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC
    GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG
    GGTACAATTA AGGTGCTGGA GTTGGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276723.1
    CDS83..2212
    Translation

    Target ORF information:

    RefSeq Version XM_022421015.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA
    CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG
    GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG
    ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGGAAAT GAGTGGCTTG
    TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC
    AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG
    ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT
    CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT
    AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT
    AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC
    ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA
    AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC
    CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT
    AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA
    GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC
    TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT
    ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA
    TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC
    TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC
    TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA
    AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA
    AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC
    TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG
    GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC
    GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG
    GGTACAATTA AGGTGCTGGA GTTGGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe289539
    Clone ID Related Accession (Same CDS sequence) XM_022421016.1
    Accession Version XM_022421016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA
    GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA
    GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC
    ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA
    TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC
    AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT
    TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT
    GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA
    TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC
    CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT
    GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT
    TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT
    TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG
    GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA
    TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA
    AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT
    TTGGCTTTTG GCTGTGCAGA GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022276724.1
    CDS89..1855
    Translation

    Target ORF information:

    RefSeq Version XM_022421016.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022421016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA
    GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA
    GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC
    ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA
    TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC
    AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT
    TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT
    GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA
    TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC
    CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT
    GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT
    TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT
    TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG
    GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA
    TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA
    AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT
    TTGGCTTTTG GCTGTGCAGA GAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37445
    Clone ID Related Accession (Same CDS sequence) XM_014115169.1
    Accession Version XM_014115169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA
    CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG
    GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG
    ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGAAAGA GCATGCAAGC
    TTGAAGAAGC AACTGGAACA GATCCAGAAG GAGCTGAGTG TTTTGGAAGA GGATATTAAA
    AGAGTGGAAG AAATGAGTGG CTTGTATTCT CCTGTCAGTG AGGATAGCAC GGTGCCTCAA
    TTTGAAGCTC CTTCTCCATC ACACAGTAAT ATTATTGATT CCACAGAATA CAGCCAACCT
    CCTGGTTTCA GTGGCAGTTC TCAGACAAAG AAGCAGCCTT GGTATAACAG CACATTAGCA
    TCACGAAGAA AACGACTCAC TGCTCATTTT GAAGACTTAG AGCAATGTTA CTTTTCTACA
    AGGATGTCTC GTATTTCAGA TGATAGTCGA ACTGCAAGCC AGTTGGATGA ATTTCAGGAA
    TGCTTGTCCA AGTTTACTCG GTATAATTCA GTACGACCTT TGGCTACATT GTCATATGCT
    AGTGATCTCT ATAATGGTTC CAGCATAGTC TCTAGTATTG AATTTGACCG AGATTGTGAC
    TATTTTGCAA TTGCTGGGGT TACAAAGAAG ATTAAAGTCT ATGAATATGG CACAGTCATT
    CAGGATGCGG TGGATATTCA TTACCCTGAA AATGAAATGA CCTGCAATTC CAAAATCAGC
    TGTATCAGTT GGAGTAGTTA CCATAAGAAT CTGTTAGCCA GCAGTGATTA TGAAGGCACT
    GTCATCCTAT GGGATGGATT CACAGGACAG AGGTCAAAGG TCTATCAGGA ACATGAGAAA
    AGGTGTTGGA GTGTTGACTT TAACTTAATG GATCCTAAAC TCTTGGCATC GGGTTCTGAT
    GATGCAAAAG TGAAGCTATG GTCTACCAAC CTAGACAACT CAGTGGCAAG CATTGAGGCG
    AAGGCTAATG TATGCTGTGT TAAATTCAGC CCTTCTTCTA GATACCATTT GGCTTTTGGC
    TGTGCAGATC ACTGTGTCCA CTACTATGAT CTTCGTAACA CTAAACAGCC AATCATGGTA
    TTCAAGGGAC ACCGAAAAGC AGTCTCTTAT GCAAAGTTTG TGAGTGGTGA GGAAATCGTC
    TCTGCCTCAA CAGACAGCCA GCTAAAGCTG TGGAATGTAG GGAAACCATA CTGTCTACGT
    TCCTTCAAGG GTCACATCAA TGAAAAAAAC TTTGTAGGCC TCGCTTCCAA TGGCGATTAT
    ATAGCTTGTG GAAGTGAGAA CAACTCTCTT TACTTATACT ATAAAGGACT TTCTAAGACT
    TTGCTAACTT TTAAGTTTGA TACGGTCAAA AGTGTTCTGG ATAAAGACCG AAAAGAAGAT
    GATACAAATG AATTTGTTAG TGCCGTGTGC TGGAGGGCAT TGCCAGATGG GGAGTCCAAT
    GTGCTGATTG CTGCTAACAG TCAGGGTACA ATTAAGGTGC TGGAGTTGGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970644.1
    CDS99..2312
    Translation

    Target ORF information:

    RefSeq Version XM_014115169.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA
    CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG
    GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG
    ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGAAAGA GCATGCAAGC
    TTGAAGAAGC AACTGGAACA GATCCAGAAG GAGCTGAGTG TTTTGGAAGA GGATATTAAA
    AGAGTGGAAG AAATGAGTGG CTTGTATTCT CCTGTCAGTG AGGATAGCAC GGTGCCTCAA
    TTTGAAGCTC CTTCTCCATC ACACAGTAAT ATTATTGATT CCACAGAATA CAGCCAACCT
    CCTGGTTTCA GTGGCAGTTC TCAGACAAAG AAGCAGCCTT GGTATAACAG CACATTAGCA
    TCACGAAGAA AACGACTCAC TGCTCATTTT GAAGACTTAG AGCAATGTTA CTTTTCTACA
    AGGATGTCTC GTATTTCAGA TGATAGTCGA ACTGCAAGCC AGTTGGATGA ATTTCAGGAA
    TGCTTGTCCA AGTTTACTCG GTATAATTCA GTACGACCTT TGGCTACATT GTCATATGCT
    AGTGATCTCT ATAATGGTTC CAGCATAGTC TCTAGTATTG AATTTGACCG AGATTGTGAC
    TATTTTGCAA TTGCTGGGGT TACAAAGAAG ATTAAAGTCT ATGAATATGG CACAGTCATT
    CAGGATGCGG TGGATATTCA TTACCCTGAA AATGAAATGA CCTGCAATTC CAAAATCAGC
    TGTATCAGTT GGAGTAGTTA CCATAAGAAT CTGTTAGCCA GCAGTGATTA TGAAGGCACT
    GTCATCCTAT GGGATGGATT CACAGGACAG AGGTCAAAGG TCTATCAGGA ACATGAGAAA
    AGGTGTTGGA GTGTTGACTT TAACTTAATG GATCCTAAAC TCTTGGCATC GGGTTCTGAT
    GATGCAAAAG TGAAGCTATG GTCTACCAAC CTAGACAACT CAGTGGCAAG CATTGAGGCG
    AAGGCTAATG TATGCTGTGT TAAATTCAGC CCTTCTTCTA GATACCATTT GGCTTTTGGC
    TGTGCAGATC ACTGTGTCCA CTACTATGAT CTTCGTAACA CTAAACAGCC AATCATGGTA
    TTCAAGGGAC ACCGAAAAGC AGTCTCTTAT GCAAAGTTTG TGAGTGGTGA GGAAATCGTC
    TCTGCCTCAA CAGACAGCCA GCTAAAGCTG TGGAATGTAG GGAAACCATA CTGTCTACGT
    TCCTTCAAGG GTCACATCAA TGAAAAAAAC TTTGTAGGCC TCGCTTCCAA TGGCGATTAT
    ATAGCTTGTG GAAGTGAGAA CAACTCTCTT TACTTATACT ATAAAGGACT TTCTAAGACT
    TTGCTAACTT TTAAGTTTGA TACGGTCAAA AGTGTTCTGG ATAAAGACCG AAAAGAAGAT
    GATACAAATG AATTTGTTAG TGCCGTGTGC TGGAGGGCAT TGCCAGATGG GGAGTCCAAT
    GTGCTGATTG CTGCTAACAG TCAGGGTACA ATTAAGGTGC TGGAGTTGGT ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37446
    Clone ID Related Accession (Same CDS sequence) XM_014115170.1
    Accession Version XM_014115170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA
    CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG
    GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG
    ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGCAACT GGAACAGATC
    CAGAAGGAGC TGAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAAAT GAGTGGCTTG
    TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC
    AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG
    ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT
    CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT
    AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT
    AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC
    ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA
    AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC
    CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT
    AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA
    GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC
    TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT
    ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA
    TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC
    TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC
    TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA
    AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA
    AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC
    TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG
    GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC
    GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG
    GGTACAATTA AGGTGCTGGA GTTGGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970645.1
    CDS99..2288
    Translation

    Target ORF information:

    RefSeq Version XM_014115170.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA
    CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG
    GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG
    ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGCAACT GGAACAGATC
    CAGAAGGAGC TGAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAAAT GAGTGGCTTG
    TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC
    AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG
    ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT
    CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT
    AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT
    AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC
    ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA
    AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC
    CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT
    AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA
    GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC
    TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT
    ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA
    TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC
    TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC
    TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA
    AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA
    AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC
    TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG
    GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC
    GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG
    GGTACAATTA AGGTGCTGGA GTTGGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37447
    Clone ID Related Accession (Same CDS sequence) XM_014115171.1 , XM_014115171.2
    Accession Version XM_014115171.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG
    ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC
    TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA
    CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT
    CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT
    GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT
    GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG
    TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC
    AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT
    ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT
    TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC
    CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC
    ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT
    AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG
    TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT
    AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC
    TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA
    GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG
    CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT
    GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC
    AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT
    ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT
    GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT
    CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970646.1
    CDS99..2231
    Translation

    Target ORF information:

    RefSeq Version XM_014115171.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115171.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG
    ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC
    TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA
    CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT
    CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT
    GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT
    GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG
    TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC
    AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT
    ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT
    TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC
    CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC
    ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT
    AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG
    TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT
    AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC
    TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA
    GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG
    CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT
    GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC
    AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT
    ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT
    GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT
    CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37447
    Clone ID Related Accession (Same CDS sequence) XM_014115171.1 , XM_014115171.2
    Accession Version XM_014115171.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2133bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG
    ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC
    TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA
    CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT
    CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT
    GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT
    GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG
    TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC
    AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT
    ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT
    TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC
    CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC
    ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT
    AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG
    TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT
    AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC
    TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA
    GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG
    CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT
    GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC
    AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT
    ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT
    GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT
    CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970646.1
    CDS83..2215
    Translation

    Target ORF information:

    RefSeq Version XM_014115171.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115171.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG
    ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC
    TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA
    CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT
    CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT
    GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT
    GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG
    TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC
    AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT
    ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT
    TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC
    CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC
    ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT
    AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG
    TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT
    AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC
    TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA
    GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG
    CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT
    GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC
    AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT
    ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT
    GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT
    CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37448
    Clone ID Related Accession (Same CDS sequence) XM_014115172.1 , XM_014115172.2
    Accession Version XM_014115172.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA
    GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA
    GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC
    ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA
    TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC
    AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT
    TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT
    GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA
    TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC
    CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT
    GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT
    TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT
    TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG
    GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA
    TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA
    AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT
    TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG
    CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT
    GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA
    TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC
    AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA
    CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC
    CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT
    GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970647.1
    CDS230..2455
    Translation

    Target ORF information:

    RefSeq Version XM_014115172.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115172.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA
    GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA
    GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC
    ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA
    TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC
    AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT
    TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT
    GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA
    TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC
    CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT
    GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT
    TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT
    TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG
    GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA
    TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA
    AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT
    TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG
    CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT
    GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA
    TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC
    AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA
    CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC
    CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT
    GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe37448
    Clone ID Related Accession (Same CDS sequence) XM_014115172.1 , XM_014115172.2
    Accession Version XM_014115172.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2226bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase RFWD2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA
    GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA
    GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC
    ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA
    TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC
    AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT
    TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT
    GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA
    TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC
    CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT
    GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT
    TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT
    TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG
    GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA
    TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA
    AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT
    TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG
    CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT
    GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA
    TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC
    AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA
    CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC
    CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT
    GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013970647.1
    CDS224..2449
    Translation

    Target ORF information:

    RefSeq Version XM_014115172.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014115172.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
    GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG
    GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT
    GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC
    GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC
    GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC
    CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC
    TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG
    TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT
    AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA
    AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG
    TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC
    AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA
    GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA
    GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA
    GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC
    ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA
    TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC
    AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT
    TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT
    GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA
    TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC
    CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT
    GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT
    TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT
    TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG
    GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA
    TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA
    AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT
    TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG
    CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT
    GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA
    TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC
    AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA
    CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC
    CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT
    GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG
    GTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.