RFWD2 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022421016.1	XP_022276724.1	E3 ubiquitin-protein ligase RFWD2 isoform X6
XM_014115172.1	XP_013970647.1	E3 ubiquitin-protein ligase RFWD2 isoform X5
XM_014115170.2	XP_013970645.2	E3 ubiquitin-protein ligase RFWD2 isoform X7
XM_014115169.1	XP_013970644.1	E3 ubiquitin-protein ligase RFWD2 isoform X1
XM_014115171.1	XP_013970646.1	E3 ubiquitin-protein ligase RFWD2 isoform X4
XM_537181.5	XP_537181.2	E3 ubiquitin-protein ligase RFWD2 isoform X2
XM_537181.6	XP_537181.2	E3 ubiquitin-protein ligase RFWD2 isoform X1
XM_022421014.1	XP_022276722.1	E3 ubiquitin-protein ligase RFWD2 isoform X2
XM_022421015.1	XP_022276723.1	E3 ubiquitin-protein ligase RFWD2 isoform X4
XM_014115172.2	XP_013970647.1	E3 ubiquitin-protein ligase RFWD2 isoform X5
XM_014115170.1	XP_013970645.1	E3 ubiquitin-protein ligase RFWD2 isoform X3
XM_014115171.2	XP_013970646.1	E3 ubiquitin-protein ligase RFWD2 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe289540	XM_014115170.2 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OCe09217	XM_537181.6 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe289537	XM_022421014.1 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe289538	XM_022421015.1 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe289539	XM_022421016.1 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OCe37445	XM_014115169.1 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe37446	XM_014115170.1 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe37447	XM_014115171.2 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe37448	XM_014115172.2 Latest version!	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
OCe09217	XM_537181.5	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe37448	XM_014115172.1	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OCe37447	XM_014115171.1	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

RFWD2 ( XM_014115170.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970645.2 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe289540
Clone ID Related Accession (Same CDS sequence)	XM_014115170.2
Accession Version	XM_014115170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115170.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAGG AAGCATACAT GACGAAATGT GGCCACAGCT TTTGCTACAA GTGTATTCAT 
CAGAGTTTGG AGGATAATAA TAGATGTCCA AAGTGTAACT ATGTTGTGGA TAATATTGAC 
CATCTGTATC CTAATTTCTT GGTGAATGAG CTTATTCTCA AACAGAAGCA AAGATTTGAG 
GAAAAGAGGT TCAAATTAGA CCACTCAAAT GGCCACAGGT GGCAAATATT ACAGGATTTG 
TTGGGAACTG ACCAAGATAA CCTTGATTTG GCCAATGTCA ACCTCATGTT GGAATTACTA 
GTGCAGAAGA AGAAACAACT GGAAGCAGAA TCACATGCAG CCCAACTACA GATCCTTATG 
GAATTCCTCA AGGTTGCAAG AAGAAATAAG AGAGAGCAAC TGGAACAGAT CCAGAAGGAG 
CTGAGTGTTT TGGAAGAGGA TATTAAAAGA GTGGAAGAAA TGAGTGGCTT GTATTCTCCT 
GTCAGTGAGG ATAGCACGGT GCCTCAATTT GAAGCTCCTT CTCCATCACA CAGTAATATT 
ATTGATTCCA CAGAATACAG CCAACCTCCT GGTTTCAGTG GCAGTTCTCA GACAAAGAAG 
CAGCCTTGGT ATAACAGCAC ATTAGCATCA CGAAGAAAAC GACTCACTGC TCATTTTGAA 
GACTTAGAGC AATGTTACTT TTCTACAAGG ATGTCTCGTA TTTCAGATGA TAGTCGAACT 
GCAAGCCAGT TGGATGAATT TCAGGAATGC TTGTCCAAGT TTACTCGGTA TAATTCAGTA 
CGACCTTTGG CTACATTGTC ATATGCTAGT GATCTCTATA ATGGTTCCAG CATAGTCTCT 
AGTATTGAAT TTGACCGAGA TTGTGACTAT TTTGCAATTG CTGGGGTTAC AAAGAAGATT 
AAAGTCTATG AATATGGCAC AGTCATTCAG GATGCGGTGG ATATTCATTA CCCTGAAAAT 
GAAATGACCT GCAATTCCAA AATCAGCTGT ATCAGTTGGA GTAGTTACCA TAAGAATCTG 
TTAGCCAGCA GTGATTATGA AGGCACTGTC ATCCTATGGG ATGGATTCAC AGGACAGAGG 
TCAAAGGTCT ATCAGGAACA TGAGAAAAGG TGTTGGAGTG TTGACTTTAA CTTAATGGAT 
CCTAAACTCT TGGCATCGGG TTCTGATGAT GCAAAAGTGA AGCTATGGTC TACCAACCTA 
GACAACTCAG TGGCAAGCAT TGAGGCGAAG GCTAATGTAT GCTGTGTTAA ATTCAGCCCT 
TCTTCTAGAT ACCATTTGGC TTTTGGCTGT GCAGATCACT GTGTCCACTA CTATGATCTT 
CGTAACACTA AACAGCCAAT CATGGTATTC AAGGGACACC GAAAAGCAGT CTCTTATGCA 
AAGTTTGTGA GTGGTGAGGA AATCGTCTCT GCCTCAACAG ACAGCCAGCT AAAGCTGTGG 
AATGTAGGGA AACCATACTG TCTACGTTCC TTCAAGGGTC ACATCAATGA AAAAAACTTT 
GTAGGCCTCG CTTCCAATGG CGATTATATA GCTTGTGGAA GTGAGAACAA CTCTCTTTAC 
TTATACTATA AAGGACTTTC TAAGACTTTG CTAACTTTTA AGTTTGATAC GGTCAAAAGT 
GTTCTGGATA AAGACCGAAA AGAAGATGAT ACAAATGAAT TTGTTAGTGC CGTGTGCTGG 
AGGGCATTGC CAGATGGGGA GTCCAATGTG CTGATTGCTG CTAACAGTCA GGGTACAATT 
AAGGTGCTGG AGTTGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970645.2
CDS	143..1903
Translation	MIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115170.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115170.2

ORF Insert Sequence:

ATGATTGAGG AAGCATACAT GACGAAATGT GGCCACAGCT TTTGCTACAA GTGTATTCAT 
CAGAGTTTGG AGGATAATAA TAGATGTCCA AAGTGTAACT ATGTTGTGGA TAATATTGAC 
CATCTGTATC CTAATTTCTT GGTGAATGAG CTTATTCTCA AACAGAAGCA AAGATTTGAG 
GAAAAGAGGT TCAAATTAGA CCACTCAAAT GGCCACAGGT GGCAAATATT ACAGGATTTG 
TTGGGAACTG ACCAAGATAA CCTTGATTTG GCCAATGTCA ACCTCATGTT GGAATTACTA 
GTGCAGAAGA AGAAACAACT GGAAGCAGAA TCACATGCAG CCCAACTACA GATCCTTATG 
GAATTCCTCA AGGTTGCAAG AAGAAATAAG AGAGAGCAAC TGGAACAGAT CCAGAAGGAG 
CTGAGTGTTT TGGAAGAGGA TATTAAAAGA GTGGAAGAAA TGAGTGGCTT GTATTCTCCT 
GTCAGTGAGG ATAGCACGGT GCCTCAATTT GAAGCTCCTT CTCCATCACA CAGTAATATT 
ATTGATTCCA CAGAATACAG CCAACCTCCT GGTTTCAGTG GCAGTTCTCA GACAAAGAAG 
CAGCCTTGGT ATAACAGCAC ATTAGCATCA CGAAGAAAAC GACTCACTGC TCATTTTGAA 
GACTTAGAGC AATGTTACTT TTCTACAAGG ATGTCTCGTA TTTCAGATGA TAGTCGAACT 
GCAAGCCAGT TGGATGAATT TCAGGAATGC TTGTCCAAGT TTACTCGGTA TAATTCAGTA 
CGACCTTTGG CTACATTGTC ATATGCTAGT GATCTCTATA ATGGTTCCAG CATAGTCTCT 
AGTATTGAAT TTGACCGAGA TTGTGACTAT TTTGCAATTG CTGGGGTTAC AAAGAAGATT 
AAAGTCTATG AATATGGCAC AGTCATTCAG GATGCGGTGG ATATTCATTA CCCTGAAAAT 
GAAATGACCT GCAATTCCAA AATCAGCTGT ATCAGTTGGA GTAGTTACCA TAAGAATCTG 
TTAGCCAGCA GTGATTATGA AGGCACTGTC ATCCTATGGG ATGGATTCAC AGGACAGAGG 
TCAAAGGTCT ATCAGGAACA TGAGAAAAGG TGTTGGAGTG TTGACTTTAA CTTAATGGAT 
CCTAAACTCT TGGCATCGGG TTCTGATGAT GCAAAAGTGA AGCTATGGTC TACCAACCTA 
GACAACTCAG TGGCAAGCAT TGAGGCGAAG GCTAATGTAT GCTGTGTTAA ATTCAGCCCT 
TCTTCTAGAT ACCATTTGGC TTTTGGCTGT GCAGATCACT GTGTCCACTA CTATGATCTT 
CGTAACACTA AACAGCCAAT CATGGTATTC AAGGGACACC GAAAAGCAGT CTCTTATGCA 
AAGTTTGTGA GTGGTGAGGA AATCGTCTCT GCCTCAACAG ACAGCCAGCT AAAGCTGTGG 
AATGTAGGGA AACCATACTG TCTACGTTCC TTCAAGGGTC ACATCAATGA AAAAAACTTT 
GTAGGCCTCG CTTCCAATGG CGATTATATA GCTTGTGGAA GTGAGAACAA CTCTCTTTAC 
TTATACTATA AAGGACTTTC TAAGACTTTG CTAACTTTTA AGTTTGATAC GGTCAAAAGT 
GTTCTGGATA AAGACCGAAA AGAAGATGAT ACAAATGAAT TTGTTAGTGC CGTGTGCTGG 
AGGGCATTGC CAGATGGGGA GTCCAATGTG CTGATTGCTG CTAACAGTCA GGGTACAATT 
AAGGTGCTGG AGTTGGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_537181.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537181.2 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe09217
Clone ID Related Accession (Same CDS sequence)	XM_537181.5 , XM_537181.6
Accession Version	XM_537181.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_537181.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA 
CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA 
ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT 
GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT 
ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG 
TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT 
AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT 
GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG 
GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT 
GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG 
AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA 
TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC 
CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA 
GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA 
TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537181.2
CDS	99..2300
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_537181.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537181.5

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA 
CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA 
ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT 
GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT 
ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG 
TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT 
AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT 
GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG 
GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT 
GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG 
AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA 
TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC 
CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA 
GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA 
TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_537181.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_537181.2 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe09217
Clone ID Related Accession (Same CDS sequence)	XM_537181.5 , XM_537181.6
Accession Version	XM_537181.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_537181.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA 
CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA 
ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT 
GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT 
ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG 
TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT 
AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT 
GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG 
GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT 
GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG 
AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA 
TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC 
CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA 
GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA 
TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_537181.2
CDS	82..2283
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_537181.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_537181.6

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGCAA 
CTGGAACAGA TCCAGAAGGA GCTGAGTGTT TTGGAAGAGG ATATTAAAAG AGTGGAAGAA 
ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT 
GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT 
ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG 
TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT 
AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT 
GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG 
GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT 
GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG 
AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA 
TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC 
CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA 
GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA 
TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_022421014.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276722.1 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe289537
Clone ID Related Accession (Same CDS sequence)	XM_022421014.1
Accession Version	XM_022421014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGGAA 
ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT 
GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT 
ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG 
TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT 
AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT 
GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG 
GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT 
GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG 
AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA 
TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC 
CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA 
GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA 
TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276722.1
CDS	83..2224
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_022421014.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421014.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGGAA 
ATGAGTGGCT TGTATTCTCC TGTCAGTGAG GATAGCACGG TGCCTCAATT TGAAGCTCCT 
TCTCCATCAC ACAGTAATAT TATTGATTCC ACAGAATACA GCCAACCTCC TGGTTTCAGT 
GGCAGTTCTC AGACAAAGAA GCAGCCTTGG TATAACAGCA CATTAGCATC ACGAAGAAAA 
CGACTCACTG CTCATTTTGA AGACTTAGAG CAATGTTACT TTTCTACAAG GATGTCTCGT 
ATTTCAGATG ATAGTCGAAC TGCAAGCCAG TTGGATGAAT TTCAGGAATG CTTGTCCAAG 
TTTACTCGGT ATAATTCAGT ACGACCTTTG GCTACATTGT CATATGCTAG TGATCTCTAT 
AATGGTTCCA GCATAGTCTC TAGTATTGAA TTTGACCGAG ATTGTGACTA TTTTGCAATT 
GCTGGGGTTA CAAAGAAGAT TAAAGTCTAT GAATATGGCA CAGTCATTCA GGATGCGGTG 
GATATTCATT ACCCTGAAAA TGAAATGACC TGCAATTCCA AAATCAGCTG TATCAGTTGG 
AGTAGTTACC ATAAGAATCT GTTAGCCAGC AGTGATTATG AAGGCACTGT CATCCTATGG 
GATGGATTCA CAGGACAGAG GTCAAAGGTC TATCAGGAAC ATGAGAAAAG GTGTTGGAGT 
GTTGACTTTA ACTTAATGGA TCCTAAACTC TTGGCATCGG GTTCTGATGA TGCAAAAGTG 
AAGCTATGGT CTACCAACCT AGACAACTCA GTGGCAAGCA TTGAGGCGAA GGCTAATGTA 
TGCTGTGTTA AATTCAGCCC TTCTTCTAGA TACCATTTGG CTTTTGGCTG TGCAGATCAC 
TGTGTCCACT ACTATGATCT TCGTAACACT AAACAGCCAA TCATGGTATT CAAGGGACAC 
CGAAAAGCAG TCTCTTATGC AAAGTTTGTG AGTGGTGAGG AAATCGTCTC TGCCTCAACA 
GACAGCCAGC TAAAGCTGTG GAATGTAGGG AAACCATACT GTCTACGTTC CTTCAAGGGT 
CACATCAATG AAAAAAACTT TGTAGGCCTC GCTTCCAATG GCGATTATAT AGCTTGTGGA 
AGTGAGAACA ACTCTCTTTA CTTATACTAT AAAGGACTTT CTAAGACTTT GCTAACTTTT 
AAGTTTGATA CGGTCAAAAG TGTTCTGGAT AAAGACCGAA AAGAAGATGA TACAAATGAA 
TTTGTTAGTG CCGTGTGCTG GAGGGCATTG CCAGATGGGG AGTCCAATGT GCTGATTGCT 
GCTAACAGTC AGGGTACAAT TAAGGTGCTG GAGTTGGTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_022421015.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276723.1 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe289538
Clone ID Related Accession (Same CDS sequence)	XM_022421015.1
Accession Version	XM_022421015.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA 
CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGGAAAT GAGTGGCTTG 
TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC 
AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG 
ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT 
CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT 
AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT 
AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC 
ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA 
AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC 
CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA 
GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC 
TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT 
ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA 
TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC 
TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA 
AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA 
AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC 
TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG 
GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC 
GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG 
GGTACAATTA AGGTGCTGGA GTTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276723.1
CDS	83..2212
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_022421015.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421015.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA 
CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGGAAAT GAGTGGCTTG 
TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC 
AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG 
ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT 
CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT 
AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT 
AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC 
ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA 
AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC 
CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA 
GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC 
TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT 
ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA 
TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC 
TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA 
AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA 
AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC 
TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG 
GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC 
GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG 
GGTACAATTA AGGTGCTGGA GTTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_022421016.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022276724.1 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe289539
Clone ID Related Accession (Same CDS sequence)	XM_022421016.1
Accession Version	XM_022421016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA 
GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA 
GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC 
ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA 
TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC 
AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT 
TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT 
GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA 
TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC 
CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT 
GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT 
TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT 
TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG 
GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA 
TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA 
AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT 
TTGGCTTTTG GCTGTGCAGA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022276724.1
CDS	89..1855
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREKEHASLKKQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCAES

Target ORF information:

RefSeq Version	XM_022421016.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022421016.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA 
GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA 
GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC 
ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA 
TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC 
AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT 
TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT 
GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA 
TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC 
CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT 
GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT 
TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT 
TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG 
GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA 
TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA 
AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT 
TTGGCTTTTG GCTGTGCAGA GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_014115169.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970644.1 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe37445
Clone ID Related Accession (Same CDS sequence)	XM_014115169.1
Accession Version	XM_014115169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA 
CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGAAAGA GCATGCAAGC 
TTGAAGAAGC AACTGGAACA GATCCAGAAG GAGCTGAGTG TTTTGGAAGA GGATATTAAA 
AGAGTGGAAG AAATGAGTGG CTTGTATTCT CCTGTCAGTG AGGATAGCAC GGTGCCTCAA 
TTTGAAGCTC CTTCTCCATC ACACAGTAAT ATTATTGATT CCACAGAATA CAGCCAACCT 
CCTGGTTTCA GTGGCAGTTC TCAGACAAAG AAGCAGCCTT GGTATAACAG CACATTAGCA 
TCACGAAGAA AACGACTCAC TGCTCATTTT GAAGACTTAG AGCAATGTTA CTTTTCTACA 
AGGATGTCTC GTATTTCAGA TGATAGTCGA ACTGCAAGCC AGTTGGATGA ATTTCAGGAA 
TGCTTGTCCA AGTTTACTCG GTATAATTCA GTACGACCTT TGGCTACATT GTCATATGCT 
AGTGATCTCT ATAATGGTTC CAGCATAGTC TCTAGTATTG AATTTGACCG AGATTGTGAC 
TATTTTGCAA TTGCTGGGGT TACAAAGAAG ATTAAAGTCT ATGAATATGG CACAGTCATT 
CAGGATGCGG TGGATATTCA TTACCCTGAA AATGAAATGA CCTGCAATTC CAAAATCAGC 
TGTATCAGTT GGAGTAGTTA CCATAAGAAT CTGTTAGCCA GCAGTGATTA TGAAGGCACT 
GTCATCCTAT GGGATGGATT CACAGGACAG AGGTCAAAGG TCTATCAGGA ACATGAGAAA 
AGGTGTTGGA GTGTTGACTT TAACTTAATG GATCCTAAAC TCTTGGCATC GGGTTCTGAT 
GATGCAAAAG TGAAGCTATG GTCTACCAAC CTAGACAACT CAGTGGCAAG CATTGAGGCG 
AAGGCTAATG TATGCTGTGT TAAATTCAGC CCTTCTTCTA GATACCATTT GGCTTTTGGC 
TGTGCAGATC ACTGTGTCCA CTACTATGAT CTTCGTAACA CTAAACAGCC AATCATGGTA 
TTCAAGGGAC ACCGAAAAGC AGTCTCTTAT GCAAAGTTTG TGAGTGGTGA GGAAATCGTC 
TCTGCCTCAA CAGACAGCCA GCTAAAGCTG TGGAATGTAG GGAAACCATA CTGTCTACGT 
TCCTTCAAGG GTCACATCAA TGAAAAAAAC TTTGTAGGCC TCGCTTCCAA TGGCGATTAT 
ATAGCTTGTG GAAGTGAGAA CAACTCTCTT TACTTATACT ATAAAGGACT TTCTAAGACT 
TTGCTAACTT TTAAGTTTGA TACGGTCAAA AGTGTTCTGG ATAAAGACCG AAAAGAAGAT 
GATACAAATG AATTTGTTAG TGCCGTGTGC TGGAGGGCAT TGCCAGATGG GGAGTCCAAT 
GTGCTGATTG CTGCTAACAG TCAGGGTACA ATTAAGGTGC TGGAGTTGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970644.1
CDS	99..2312
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREKEHASLKKQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115169.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115169.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA 
CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGAAAGA GCATGCAAGC 
TTGAAGAAGC AACTGGAACA GATCCAGAAG GAGCTGAGTG TTTTGGAAGA GGATATTAAA 
AGAGTGGAAG AAATGAGTGG CTTGTATTCT CCTGTCAGTG AGGATAGCAC GGTGCCTCAA 
TTTGAAGCTC CTTCTCCATC ACACAGTAAT ATTATTGATT CCACAGAATA CAGCCAACCT 
CCTGGTTTCA GTGGCAGTTC TCAGACAAAG AAGCAGCCTT GGTATAACAG CACATTAGCA 
TCACGAAGAA AACGACTCAC TGCTCATTTT GAAGACTTAG AGCAATGTTA CTTTTCTACA 
AGGATGTCTC GTATTTCAGA TGATAGTCGA ACTGCAAGCC AGTTGGATGA ATTTCAGGAA 
TGCTTGTCCA AGTTTACTCG GTATAATTCA GTACGACCTT TGGCTACATT GTCATATGCT 
AGTGATCTCT ATAATGGTTC CAGCATAGTC TCTAGTATTG AATTTGACCG AGATTGTGAC 
TATTTTGCAA TTGCTGGGGT TACAAAGAAG ATTAAAGTCT ATGAATATGG CACAGTCATT 
CAGGATGCGG TGGATATTCA TTACCCTGAA AATGAAATGA CCTGCAATTC CAAAATCAGC 
TGTATCAGTT GGAGTAGTTA CCATAAGAAT CTGTTAGCCA GCAGTGATTA TGAAGGCACT 
GTCATCCTAT GGGATGGATT CACAGGACAG AGGTCAAAGG TCTATCAGGA ACATGAGAAA 
AGGTGTTGGA GTGTTGACTT TAACTTAATG GATCCTAAAC TCTTGGCATC GGGTTCTGAT 
GATGCAAAAG TGAAGCTATG GTCTACCAAC CTAGACAACT CAGTGGCAAG CATTGAGGCG 
AAGGCTAATG TATGCTGTGT TAAATTCAGC CCTTCTTCTA GATACCATTT GGCTTTTGGC 
TGTGCAGATC ACTGTGTCCA CTACTATGAT CTTCGTAACA CTAAACAGCC AATCATGGTA 
TTCAAGGGAC ACCGAAAAGC AGTCTCTTAT GCAAAGTTTG TGAGTGGTGA GGAAATCGTC 
TCTGCCTCAA CAGACAGCCA GCTAAAGCTG TGGAATGTAG GGAAACCATA CTGTCTACGT 
TCCTTCAAGG GTCACATCAA TGAAAAAAAC TTTGTAGGCC TCGCTTCCAA TGGCGATTAT 
ATAGCTTGTG GAAGTGAGAA CAACTCTCTT TACTTATACT ATAAAGGACT TTCTAAGACT 
TTGCTAACTT TTAAGTTTGA TACGGTCAAA AGTGTTCTGG ATAAAGACCG AAAAGAAGAT 
GATACAAATG AATTTGTTAG TGCCGTGTGC TGGAGGGCAT TGCCAGATGG GGAGTCCAAT 
GTGCTGATTG CTGCTAACAG TCAGGGTACA ATTAAGGTGC TGGAGTTGGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_014115170.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970645.1 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe37446
Clone ID Related Accession (Same CDS sequence)	XM_014115170.1
Accession Version	XM_014115170.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA 
CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGCAACT GGAACAGATC 
CAGAAGGAGC TGAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAAAT GAGTGGCTTG 
TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC 
AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG 
ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT 
CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT 
AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT 
AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC 
ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA 
AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC 
CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA 
GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC 
TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT 
ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA 
TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC 
TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA 
AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA 
AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC 
TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG 
GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC 
GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG 
GGTACAATTA AGGTGCTGGA GTTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970645.1
CDS	99..2288
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115170.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115170.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAAATG GCCACAGGTG GCAAATATTA 
CAGGATTTGT TGGGAACTGA CCAAGATAAC CTTGATTTGG CCAATGTCAA CCTCATGTTG 
GAATTACTAG TGCAGAAGAA GAAACAACTG GAAGCAGAAT CACATGCAGC CCAACTACAG 
ATCCTTATGG AATTCCTCAA GGTTGCAAGA AGAAATAAGA GAGAGCAACT GGAACAGATC 
CAGAAGGAGC TGAGTGTTTT GGAAGAGGAT ATTAAAAGAG TGGAAGAAAT GAGTGGCTTG 
TATTCTCCTG TCAGTGAGGA TAGCACGGTG CCTCAATTTG AAGCTCCTTC TCCATCACAC 
AGTAATATTA TTGATTCCAC AGAATACAGC CAACCTCCTG GTTTCAGTGG CAGTTCTCAG 
ACAAAGAAGC AGCCTTGGTA TAACAGCACA TTAGCATCAC GAAGAAAACG ACTCACTGCT 
CATTTTGAAG ACTTAGAGCA ATGTTACTTT TCTACAAGGA TGTCTCGTAT TTCAGATGAT 
AGTCGAACTG CAAGCCAGTT GGATGAATTT CAGGAATGCT TGTCCAAGTT TACTCGGTAT 
AATTCAGTAC GACCTTTGGC TACATTGTCA TATGCTAGTG ATCTCTATAA TGGTTCCAGC 
ATAGTCTCTA GTATTGAATT TGACCGAGAT TGTGACTATT TTGCAATTGC TGGGGTTACA 
AAGAAGATTA AAGTCTATGA ATATGGCACA GTCATTCAGG ATGCGGTGGA TATTCATTAC 
CCTGAAAATG AAATGACCTG CAATTCCAAA ATCAGCTGTA TCAGTTGGAG TAGTTACCAT 
AAGAATCTGT TAGCCAGCAG TGATTATGAA GGCACTGTCA TCCTATGGGA TGGATTCACA 
GGACAGAGGT CAAAGGTCTA TCAGGAACAT GAGAAAAGGT GTTGGAGTGT TGACTTTAAC 
TTAATGGATC CTAAACTCTT GGCATCGGGT TCTGATGATG CAAAAGTGAA GCTATGGTCT 
ACCAACCTAG ACAACTCAGT GGCAAGCATT GAGGCGAAGG CTAATGTATG CTGTGTTAAA 
TTCAGCCCTT CTTCTAGATA CCATTTGGCT TTTGGCTGTG CAGATCACTG TGTCCACTAC 
TATGATCTTC GTAACACTAA ACAGCCAATC ATGGTATTCA AGGGACACCG AAAAGCAGTC 
TCTTATGCAA AGTTTGTGAG TGGTGAGGAA ATCGTCTCTG CCTCAACAGA CAGCCAGCTA 
AAGCTGTGGA ATGTAGGGAA ACCATACTGT CTACGTTCCT TCAAGGGTCA CATCAATGAA 
AAAAACTTTG TAGGCCTCGC TTCCAATGGC GATTATATAG CTTGTGGAAG TGAGAACAAC 
TCTCTTTACT TATACTATAA AGGACTTTCT AAGACTTTGC TAACTTTTAA GTTTGATACG 
GTCAAAAGTG TTCTGGATAA AGACCGAAAA GAAGATGATA CAAATGAATT TGTTAGTGCC 
GTGTGCTGGA GGGCATTGCC AGATGGGGAG TCCAATGTGC TGATTGCTGC TAACAGTCAG 
GGTACAATTA AGGTGCTGGA GTTGGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_014115171.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970646.1 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe37447
Clone ID Related Accession (Same CDS sequence)	XM_014115171.1 , XM_014115171.2
Accession Version	XM_014115171.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG 
ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC 
TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA 
CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT 
CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT 
GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT 
GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG 
TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC 
AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT 
ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT 
TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC 
CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC 
ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT 
AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG 
TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT 
AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC 
TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA 
GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG 
CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT 
GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC 
AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT 
ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT 
GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT 
CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970646.1
CDS	99..2231
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115171.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115171.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG 
ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC 
TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA 
CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT 
CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT 
GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT 
GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG 
TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC 
AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT 
ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT 
TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC 
CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC 
ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT 
AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG 
TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT 
AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC 
TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA 
GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG 
CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT 
GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC 
AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT 
ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT 
GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT 
CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_014115171.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970646.1 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe37447
Clone ID Related Accession (Same CDS sequence)	XM_014115171.1 , XM_014115171.2
Accession Version	XM_014115171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG 
ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC 
TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA 
CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT 
CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT 
GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT 
GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG 
TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC 
AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT 
ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT 
TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC 
CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC 
ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT 
AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG 
TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT 
AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC 
TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA 
GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG 
CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT 
GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC 
AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT 
ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT 
GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT 
CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970646.1
CDS	83..2215
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115171.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115171.2

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCACA ACTGGAACAG 
ATCCAGAAGG AGCTGAGTGT TTTGGAAGAG GATATTAAAA GAGTGGAAGA AATGAGTGGC 
TTGTATTCTC CTGTCAGTGA GGATAGCACG GTGCCTCAAT TTGAAGCTCC TTCTCCATCA 
CACAGTAATA TTATTGATTC CACAGAATAC AGCCAACCTC CTGGTTTCAG TGGCAGTTCT 
CAGACAAAGA AGCAGCCTTG GTATAACAGC ACATTAGCAT CACGAAGAAA ACGACTCACT 
GCTCATTTTG AAGACTTAGA GCAATGTTAC TTTTCTACAA GGATGTCTCG TATTTCAGAT 
GATAGTCGAA CTGCAAGCCA GTTGGATGAA TTTCAGGAAT GCTTGTCCAA GTTTACTCGG 
TATAATTCAG TACGACCTTT GGCTACATTG TCATATGCTA GTGATCTCTA TAATGGTTCC 
AGCATAGTCT CTAGTATTGA ATTTGACCGA GATTGTGACT ATTTTGCAAT TGCTGGGGTT 
ACAAAGAAGA TTAAAGTCTA TGAATATGGC ACAGTCATTC AGGATGCGGT GGATATTCAT 
TACCCTGAAA ATGAAATGAC CTGCAATTCC AAAATCAGCT GTATCAGTTG GAGTAGTTAC 
CATAAGAATC TGTTAGCCAG CAGTGATTAT GAAGGCACTG TCATCCTATG GGATGGATTC 
ACAGGACAGA GGTCAAAGGT CTATCAGGAA CATGAGAAAA GGTGTTGGAG TGTTGACTTT 
AACTTAATGG ATCCTAAACT CTTGGCATCG GGTTCTGATG ATGCAAAAGT GAAGCTATGG 
TCTACCAACC TAGACAACTC AGTGGCAAGC ATTGAGGCGA AGGCTAATGT ATGCTGTGTT 
AAATTCAGCC CTTCTTCTAG ATACCATTTG GCTTTTGGCT GTGCAGATCA CTGTGTCCAC 
TACTATGATC TTCGTAACAC TAAACAGCCA ATCATGGTAT TCAAGGGACA CCGAAAAGCA 
GTCTCTTATG CAAAGTTTGT GAGTGGTGAG GAAATCGTCT CTGCCTCAAC AGACAGCCAG 
CTAAAGCTGT GGAATGTAGG GAAACCATAC TGTCTACGTT CCTTCAAGGG TCACATCAAT 
GAAAAAAACT TTGTAGGCCT CGCTTCCAAT GGCGATTATA TAGCTTGTGG AAGTGAGAAC 
AACTCTCTTT ACTTATACTA TAAAGGACTT TCTAAGACTT TGCTAACTTT TAAGTTTGAT 
ACGGTCAAAA GTGTTCTGGA TAAAGACCGA AAAGAAGATG ATACAAATGA ATTTGTTAGT 
GCCGTGTGCT GGAGGGCATT GCCAGATGGG GAGTCCAATG TGCTGATTGC TGCTAACAGT 
CAGGGTACAA TTAAGGTGCT GGAGTTGGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_014115172.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970647.1 Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe37448
Clone ID Related Accession (Same CDS sequence)	XM_014115172.1 , XM_014115172.2
Accession Version	XM_014115172.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726067.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA 
GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA 
GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC 
ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA 
TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC 
AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT 
TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT 
GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA 
TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC 
CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT 
GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT 
TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT 
TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG 
GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA 
TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA 
AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT 
TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG 
CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT 
GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA 
TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC 
AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA 
CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC 
CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT 
GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970647.1
CDS	230..2455
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREKEHASLKKQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115172.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2, E3 ubiquitin protein ligase (RFWD2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115172.1

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA 
GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA 
GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC 
ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA 
TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC 
AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT 
TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT 
GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA 
TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC 
CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT 
GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT 
TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT 
TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG 
GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA 
TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA 
AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT 
TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG 
CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT 
GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA 
TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC 
AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA 
CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC 
CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT 
GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD2 ( XM_014115172.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013970647.1 Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe37448
Clone ID Related Accession (Same CDS sequence)	XM_014115172.1 , XM_014115172.2
Accession Version	XM_014115172.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	E3 ubiquitin-protein ligase RFWD2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006589.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014115172.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA 
GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA 
GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC 
ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA 
TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC 
AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT 
TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT 
GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA 
TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC 
CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT 
GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT 
TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT 
TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG 
GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA 
TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA 
AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT 
TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG 
CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT 
GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA 
TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC 
AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA 
CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC 
CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT 
GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013970647.1
CDS	224..2449
Translation	MSGSRQVGSGSAGTSLGSSAASSVTSASSSLSSSPSPPSVAAAVAVAAASGGSAQAAGSGGLGGPVARPVLVAAAVSVSGDGAVSASQSRLSCAARPSAGVGGSSSGLGSGSRKRPLLAPLCNGLINSYEGKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQILQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREKEHASLKKQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSNIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFSPSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQLKLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Target ORF information:

RefSeq Version	XM_014115172.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ring finger and WD repeat domain 2 (RFWD2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014115172.2

ORF Insert Sequence:

ATGTCCGGGA GCCGCCAGGT CGGGTCGGGT TCCGCCGGGA CCAGCCTCGG CTCCTCGGCG 
GCCTCCTCGG TGACTTCCGC CTCCTCGTCC TTGTCCTCCT CCCCGTCGCC CCCTTCGGTG 
GCGGCGGCGG TTGCGGTGGC GGCTGCGTCT GGCGGGTCGG CCCAGGCCGC GGGGTCGGGT 
GGCCTCGGAG GCCCGGTGGC GCGGCCTGTG CTGGTGGCGG CCGCCGTGTC GGTCAGCGGC 
GATGGGGCGG TGTCCGCGAG CCAGTCTCGG CTCAGCTGCG CGGCCAGGCC CAGCGCCGGC 
GTGGGAGGGA GCAGCTCCGG CCTCGGCAGC GGCAGCCGGA AGAGACCTCT GCTCGCCCCC 
CTGTGCAACG GGCTCATCAA CTCCTACGAG GGCAAAAGCA ACGACTTCGT GTGCCCTATC 
TGCTTTGATA TGATTGAGGA AGCATACATG ACGAAATGTG GCCACAGCTT TTGCTACAAG 
TGTATTCATC AGAGTTTGGA GGATAATAAT AGATGTCCAA AGTGTAACTA TGTTGTGGAT 
AATATTGACC ATCTGTATCC TAATTTCTTG GTGAATGAGC TTATTCTCAA ACAGAAGCAA 
AGATTTGAGG AAAAGAGGTT CAAATTAGAC CACTCAGTGA GTAGCACCAA TGGCCACAGG 
TGGCAAATAT TACAGGATTT GTTGGGAACT GACCAAGATA ACCTTGATTT GGCCAATGTC 
AACCTCATGT TGGAATTACT AGTGCAGAAG AAGAAACAAC TGGAAGCAGA ATCACATGCA 
GCCCAACTAC AGATCCTTAT GGAATTCCTC AAGGTTGCAA GAAGAAATAA GAGAGAGAAA 
GAGCATGCAA GCTTGAAGAA GCAACTGGAA CAGATCCAGA AGGAGCTGAG TGTTTTGGAA 
GAGGATATTA AAAGAGTGGA AGAAATGAGT GGCTTGTATT CTCCTGTCAG TGAGGATAGC 
ACGGTGCCTC AATTTGAAGC TCCTTCTCCA TCACACAGTA ATATTATTGA TTCCACAGAA 
TACAGCCAAC CTCCTGGTTT CAGTGGCAGT TCTCAGACAA AGAAGCAGCC TTGGTATAAC 
AGCACATTAG CATCACGAAG AAAACGACTC ACTGCTCATT TTGAAGACTT AGAGCAATGT 
TACTTTTCTA CAAGGATGTC TCGTATTTCA GATGATAGTC GAACTGCAAG CCAGTTGGAT 
GAATTTCAGG AATGCTTGTC CAAGTTTACT CGGTATAATT CAGTACGACC TTTGGCTACA 
TTGTCATATG CTAGTGATCT CTATAATGGT TCCAGCATAG TCTCTAGTAT TGAATTTGAC 
CGAGATTGTG ACTATTTTGC AATTGCTGGG GTTACAAAGA AGATTAAAGT CTATGAATAT 
GGCACAGTCA TTCAGGATGC GGTGGATATT CATTACCCTG AAAATGAAAT GACCTGCAAT 
TCCAAAATCA GCTGTATCAG TTGGAGTAGT TACCATAAGA ATCTGTTAGC CAGCAGTGAT 
TATGAAGGCA CTGTCATCCT ATGGGATGGA TTCACAGGAC AGAGGTCAAA GGTCTATCAG 
GAACATGAGA AAAGGTGTTG GAGTGTTGAC TTTAACTTAA TGGATCCTAA ACTCTTGGCA 
TCGGGTTCTG ATGATGCAAA AGTGAAGCTA TGGTCTACCA ACCTAGACAA CTCAGTGGCA 
AGCATTGAGG CGAAGGCTAA TGTATGCTGT GTTAAATTCA GCCCTTCTTC TAGATACCAT 
TTGGCTTTTG GCTGTGCAGA TCACTGTGTC CACTACTATG ATCTTCGTAA CACTAAACAG 
CCAATCATGG TATTCAAGGG ACACCGAAAA GCAGTCTCTT ATGCAAAGTT TGTGAGTGGT 
GAGGAAATCG TCTCTGCCTC AACAGACAGC CAGCTAAAGC TGTGGAATGT AGGGAAACCA 
TACTGTCTAC GTTCCTTCAA GGGTCACATC AATGAAAAAA ACTTTGTAGG CCTCGCTTCC 
AATGGCGATT ATATAGCTTG TGGAAGTGAG AACAACTCTC TTTACTTATA CTATAAAGGA 
CTTTCTAAGA CTTTGCTAAC TTTTAAGTTT GATACGGTCA AAAGTGTTCT GGATAAAGAC 
CGAAAAGAAG ATGATACAAA TGAATTTGTT AGTGCCGTGT GCTGGAGGGC ATTGCCAGAT 
GGGGAGTCCA ATGTGCTGAT TGCTGCTAAC AGTCAGGGTA CAATTAAGGT GCTGGAGTTG 
GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RFWD2
Entrez Gene ID	480060
Full Name	COP1, E3 ubiquitin ligase
Synonyms	RFWD2
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Pan troglodytes (chimpanzee)	RFWD2	XP_514018.2
Danio rerio (zebrafish)	rfwd2	NP_001083011.1
Oryza sativa (rice)	Os02g0771100	NP_001048254.1
Canis lupus familiaris (dog)	RFWD2	XP_537181.2
Bos taurus (cattle)	RFWD2	NP_001096726.1
Rattus norvegicus (Norway rat)	Rfwd2	NP_001020297.1
Homo sapiens (human)	RFWD2	NP_071902.2
	RFWD2	XP_001104796.1
Mus musculus (house mouse)	Rfwd2	NP_036061.1
Gallus gallus (chicken)	RFWD2	XP_004943320.1
Xenopus tropicalis (tropical clawed frog)	rfwd2	NP_001016199.1
Arabidopsis thaliana (thale cress)	COP1	NP_180854.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RFWD2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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