HERC4 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022417474.1	XP_022273182.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_022417476.1	XP_022273184.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_005618937.3	XP_005618994.1	probable E3 ubiquitin-protein ligase HERC4 isoform X6
XM_844715.5	XP_849808.1	probable E3 ubiquitin-protein ligase HERC4 isoform X7
XM_022417482.1	XP_022273190.1	probable E3 ubiquitin-protein ligase HERC4 isoform X10
XM_022417484.1	XP_022273192.1	probable E3 ubiquitin-protein ligase HERC4 isoform X12
XM_014112840.1	XP_013968315.1	probable E3 ubiquitin-protein ligase HERC4 isoform X2
XM_844715.4	XP_849808.1	probable E3 ubiquitin-protein ligase HERC4 isoform X5
XM_014112841.1	XP_013968316.1	probable E3 ubiquitin-protein ligase HERC4 isoform X7
XM_536367.5	XP_536367.2	probable E3 ubiquitin-protein ligase HERC4 isoform X6
XM_022417477.1	XP_022273185.1	probable E3 ubiquitin-protein ligase HERC4 isoform X3
XM_022417479.1	XP_022273187.1	probable E3 ubiquitin-protein ligase HERC4 isoform X8
XM_022417480.1	XP_022273188.1	probable E3 ubiquitin-protein ligase HERC4 isoform X10
XM_022417478.1	XP_022273186.1	probable E3 ubiquitin-protein ligase HERC4 isoform X4
XM_014112839.1	XP_013968314.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_005618937.2	XP_005618994.1	probable E3 ubiquitin-protein ligase HERC4 isoform X4
XM_014112838.2	XP_013968313.1	probable E3 ubiquitin-protein ligase HERC4 isoform X2
XM_005618934.3	XP_005618991.1	probable E3 ubiquitin-protein ligase HERC4 isoform X5
XM_014112838.1	XP_013968313.1	probable E3 ubiquitin-protein ligase HERC4 isoform X1
XM_005618934.2	XP_005618991.1	probable E3 ubiquitin-protein ligase HERC4 isoform X3
XM_014112842.1	XP_013968317.1	probable E3 ubiquitin-protein ligase HERC4 isoform X8
XM_536367.6	XP_536367.2	probable E3 ubiquitin-protein ligase HERC4 isoform X9
XM_022417483.1	XP_022273191.1	probable E3 ubiquitin-protein ligase HERC4 isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe04937	XM_022417484.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe287516	XM_022417474.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287516	XM_022417476.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287517	XM_022417477.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287518	XM_022417478.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287519	XM_022417479.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287520	XM_022417480.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287520	XM_022417482.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe287521	XM_022417483.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe35983	XM_014112838.2 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe35983	XM_014112839.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe35984	XM_014112840.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe35985	XM_014112841.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe35986	XM_014112842.1 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe04930	XM_005618934.3 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04932	XM_005618937.3 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04933	XM_844715.5 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04934	XM_536367.6 Latest version!	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OCe04933	XM_844715.4	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04932	XM_005618937.2	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe35983	XM_014112838.1	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe04930	XM_005618934.2	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OCe04934	XM_536367.5	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

HERC4 ( XM_022417484.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273192.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OCe04937
Clone ID Related Accession (Same CDS sequence)	XM_022417484.1
Accession Version	XM_022417484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAAA TGGAAGGTGG CGTCTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGT 
CATAACTCTA CCAGTCATGA AATAAACCCA CGGAAAGTTT TTGAACTTAT GGGAAGCATT 
GTTACTCAGA TTGCTTGTGG AAGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA 
ATTTATTCTT TTGGACTTGG TGGTAATGGG CAGCTAGGAA CTGGTTCAAC AAGCAACAGG 
AAAAGTCCTT TTACTGTGAA AGGAAATTGG TTCCCTTATA ATGGGCAGTG TCCACCAGAT 
ATTGATTCTG AAGAATATTT CTGTGTGAAA AGAATTTTCT CAGGTGGAGA TCAAAGCTTT 
TCACATTACT CTAATCCCCA GAACTGTGGG CCACCAGATG ACTTTAGATG TCCTGATCCT 
TCAAAGCAGA TCTGGACTGT GAATGAAACT CTAATTCAAA AATGGCTGAG CTACCCATCT 
GGGAGGTTTC CTGTGGAGAT AGCCAATGAA ATAGATGGAA CATTTTCTTC CTCTGGCTGT 
CTAAATGGAA GTTTTCTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG CACCAAGTTT 
TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT 
CCTCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT 
AGTTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG 
ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTAAAT 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TTGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGCATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TATTGCATAA GGTAAATGAG AAAACGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGGA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGCAC ATATCCATTT 
GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAAACAG ATGCAGTCTT ACAGATGCAG 
ATGGCTATTG ACCAGGCCCA CAGACAGAAT GTCTCCTCTC TTTTCCTCCC AGTGATTGAA 
TCTGTGAATC CCTGCTTAAT TCTAGTTGTA CGTAGAGAAA ATATTGTAGG AGATGCAATG 
GAAGTCCTTA GGAAAACAAA GAATATAGAC TACAAAAAGC CACTCAAGGT TATATTTGTT 
GGAGAAGATG CTGTTGATGC AGGAGGAGTA CGCAAAGAAT TTTTCTTGCT CATCATGAGA 
GAATTGTTGG ATCCTAAATA TGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG 
TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTTCATT TGATTGGTGT TATCTGTGGA 
TTAGCAATTT ATAATTTTAC TATTGTGGAC CTCCACTTTC CTTTGGCTTT ATATAAGAAA 
CTGCTGAAAA AGAAGCCATC CTTGGATGAT TTGAAGGAAC TAGTGCCTGA TGTTGGGAGA 
AGCATGCAGC AATTACTGGA TTATCCAGAA GATGATGTTG AGGAAACATT TTGTCTTAAT 
TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT 
GCAGACACAG CTGTTAACAA ACAAAATCGG CAGGAGTTTG TTGATGCTTA TGTGGATTAC 
ATATTCAATA AATCAGTGGC TTCCTTGTTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC 
TGTGGAGGAA AGGTCCTTCA GCTCTTCCAG CCAAATGAAC TACAAGCAAT GGTTATTGGA 
AACACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAGTATTGG 
GCAGAGCATC CAACGATAAA AATTTTTTGG GAAGTATTTC ATGAATTACC ATTGGAAAAG 
AAAAAACAAT TTCTGTTATT TTTGACAGGT AGTGATCGAA TACCTATTCT TGGTATGAAG 
AGTCTAAAAC TAGTCATCCA GTCAACAGGA GGTGGTGAGG AATATCTCCC AGTTTCCCAT 
ACCTGTTTTA ATCTTCTGGA TCTTCCAAAA TACACCGAAA AAGAAACTCT ACGCTCTAAG 
CTGATTCAAG CAATTGATCA TAATGAAGGC TTCAGTTTAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273192.1
CDS	145..2529
Translation	MMKMEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417484.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417484.1

ORF Insert Sequence:

ATGATGAAAA TGGAAGGTGG CGTCTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGT 
CATAACTCTA CCAGTCATGA AATAAACCCA CGGAAAGTTT TTGAACTTAT GGGAAGCATT 
GTTACTCAGA TTGCTTGTGG AAGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA 
ATTTATTCTT TTGGACTTGG TGGTAATGGG CAGCTAGGAA CTGGTTCAAC AAGCAACAGG 
AAAAGTCCTT TTACTGTGAA AGGAAATTGG TTCCCTTATA ATGGGCAGTG TCCACCAGAT 
ATTGATTCTG AAGAATATTT CTGTGTGAAA AGAATTTTCT CAGGTGGAGA TCAAAGCTTT 
TCACATTACT CTAATCCCCA GAACTGTGGG CCACCAGATG ACTTTAGATG TCCTGATCCT 
TCAAAGCAGA TCTGGACTGT GAATGAAACT CTAATTCAAA AATGGCTGAG CTACCCATCT 
GGGAGGTTTC CTGTGGAGAT AGCCAATGAA ATAGATGGAA CATTTTCTTC CTCTGGCTGT 
CTAAATGGAA GTTTTCTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG CACCAAGTTT 
TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT 
CCTCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT 
AGTTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG 
ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTAAAT 
CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA 
TTCCTCAAGA TTGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG 
ATCGGCATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG 
GTTTTAGAAA TATTGCATAA GGTAAATGAG AAAACGGGAC AGATTATACA GTATGATAAA 
TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGGA ATGATTATAT CAACTGGGTC 
CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGCAC ATATCCATTT 
GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAAACAG ATGCAGTCTT ACAGATGCAG 
ATGGCTATTG ACCAGGCCCA CAGACAGAAT GTCTCCTCTC TTTTCCTCCC AGTGATTGAA 
TCTGTGAATC CCTGCTTAAT TCTAGTTGTA CGTAGAGAAA ATATTGTAGG AGATGCAATG 
GAAGTCCTTA GGAAAACAAA GAATATAGAC TACAAAAAGC CACTCAAGGT TATATTTGTT 
GGAGAAGATG CTGTTGATGC AGGAGGAGTA CGCAAAGAAT TTTTCTTGCT CATCATGAGA 
GAATTGTTGG ATCCTAAATA TGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG 
TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTTCATT TGATTGGTGT TATCTGTGGA 
TTAGCAATTT ATAATTTTAC TATTGTGGAC CTCCACTTTC CTTTGGCTTT ATATAAGAAA 
CTGCTGAAAA AGAAGCCATC CTTGGATGAT TTGAAGGAAC TAGTGCCTGA TGTTGGGAGA 
AGCATGCAGC AATTACTGGA TTATCCAGAA GATGATGTTG AGGAAACATT TTGTCTTAAT 
TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT 
GCAGACACAG CTGTTAACAA ACAAAATCGG CAGGAGTTTG TTGATGCTTA TGTGGATTAC 
ATATTCAATA AATCAGTGGC TTCCTTGTTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC 
TGTGGAGGAA AGGTCCTTCA GCTCTTCCAG CCAAATGAAC TACAAGCAAT GGTTATTGGA 
AACACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAGTATTGG 
GCAGAGCATC CAACGATAAA AATTTTTTGG GAAGTATTTC ATGAATTACC ATTGGAAAAG 
AAAAAACAAT TTCTGTTATT TTTGACAGGT AGTGATCGAA TACCTATTCT TGGTATGAAG 
AGTCTAAAAC TAGTCATCCA GTCAACAGGA GGTGGTGAGG AATATCTCCC AGTTTCCCAT 
ACCTGTTTTA ATCTTCTGGA TCTTCCAAAA TACACCGAAA AAGAAACTCT ACGCTCTAAG 
CTGATTCAAG CAATTGATCA TAATGAAGGC TTCAGTTTAA TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417474.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273182.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe287516
Clone ID Related Accession (Same CDS sequence)	XM_022417474.1 , XM_022417476.1
Accession Version	XM_022417474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273182.1
CDS	455..3760
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417474.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417474.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417476.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273184.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe287516
Clone ID Related Accession (Same CDS sequence)	XM_022417474.1 , XM_022417476.1
Accession Version	XM_022417476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273184.1
CDS	164..3469
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417476.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417476.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417477.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273185.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe287517
Clone ID Related Accession (Same CDS sequence)	XM_022417477.1
Accession Version	XM_022417477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG 
GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA 
TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA 
GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC 
ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA 
GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA 
AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT 
CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC 
TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG 
ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273185.1
CDS	454..3735
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417477.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417477.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG 
GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA 
TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA 
GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC 
ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA 
GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA 
AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT 
CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC 
TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG 
ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417478.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273186.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

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CloneID	OCe287518
Clone ID Related Accession (Same CDS sequence)	XM_022417478.1
Accession Version	XM_022417478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG 
GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGGT CAGGTGGCAG TATCAGAAAT 
ATTAAAAGTT TGTCAGATAT CCAGATAGTA CAGGTTGCTT GCGGTTACTA TCATTCACTT 
GCACTTTCTA AAGCGAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAGTTGGGT 
TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT GCTTGGAATT 
CCTTTCATGC AAGTTGCAGC AGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT 
ATTTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG 
TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA 
GAAGATCATA CTGCAGCACT AACCAAGGAA GGTGGCGTCT TTACTTTTGG AGCTGGAGGG 
TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCACGGAA AGTTTTTGAA 
CTTATGGGAA GCATTGTTAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT 
CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGGCAGCT AGGAACTGGT 
TCAACAAGCA ACAGGAAAAG TCCTTTTACT GTGAAAGGAA ATTGGTTCCC TTATAATGGG 
CAGTGTCCAC CAGATATTGA TTCTGAAGAA TATTTCTGTG TGAAAAGAAT TTTCTCAGGT 
GGAGATCAAA GCTTTTCACA TTACTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT 
AGATGTCCTG ATCCTTCAAA GCAGATCTGG ACTGTGAATG AAACTCTAAT TCAAAAATGG 
CTGAGCTACC CATCTGGGAG GTTTCCTGTG GAGATAGCCA ATGAAATAGA TGGAACATTT 
TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT CTAGCTGTTA GCAATGATGA TCACTATAGA 
ACAGGCACCA AGTTTTCAGG GGTTGATATG AATGCTGCTA GGCTTTTATT CCACAAACTT 
ATACAACCTG ATCATCCTCA GATATCTCAG CAGGTGGCAG CTAGTTTGGA AAAGAATCTT 
ATTCCTAAAC TGACTAGTTC CTTACCTGAT GTTGAAGCAT TGAGGTTTTA TCTTACTCTA 
CCAGAATGTC CCCTGATGAG TGATTCCAAC AATTTCACAA CAATAGCAAT TCCCTTTGGT 
ACAGCTCTTG TAAATCTAGA AAAGGCACCA CTGAAAGTAC TTGAAAACTG GTGGTCAGTA 
CTTGAACCTC CACTATTCCT CAAGATTGTA GAACTTTTTA AGGAAGTTGT GGTACATCTT 
TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAAAGAA GAATTTTCAA CAGTTTTCTT 
CATACTGCAT TAAAGGTTTT AGAAATATTG CATAAGGTAA ATGAGAAAAC GGGACAGATT 
ATACAGTATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT AAGGAATGAT 
TATATCAACT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG 
TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA 
ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC 
AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT 
CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG 
AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA 
GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT 
GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA 
GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT 
ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC 
TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT 
TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC 
TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA 
CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT 
TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG 
CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG 
GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA 
ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT 
TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG 
TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT 
CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT 
AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273186.1
CDS	455..3718
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417478.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417478.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG 
GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGGT CAGGTGGCAG TATCAGAAAT 
ATTAAAAGTT TGTCAGATAT CCAGATAGTA CAGGTTGCTT GCGGTTACTA TCATTCACTT 
GCACTTTCTA AAGCGAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAGTTGGGT 
TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT GCTTGGAATT 
CCTTTCATGC AAGTTGCAGC AGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT 
ATTTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG 
TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA 
GAAGATCATA CTGCAGCACT AACCAAGGAA GGTGGCGTCT TTACTTTTGG AGCTGGAGGG 
TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCACGGAA AGTTTTTGAA 
CTTATGGGAA GCATTGTTAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT 
CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGGCAGCT AGGAACTGGT 
TCAACAAGCA ACAGGAAAAG TCCTTTTACT GTGAAAGGAA ATTGGTTCCC TTATAATGGG 
CAGTGTCCAC CAGATATTGA TTCTGAAGAA TATTTCTGTG TGAAAAGAAT TTTCTCAGGT 
GGAGATCAAA GCTTTTCACA TTACTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT 
AGATGTCCTG ATCCTTCAAA GCAGATCTGG ACTGTGAATG AAACTCTAAT TCAAAAATGG 
CTGAGCTACC CATCTGGGAG GTTTCCTGTG GAGATAGCCA ATGAAATAGA TGGAACATTT 
TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT CTAGCTGTTA GCAATGATGA TCACTATAGA 
ACAGGCACCA AGTTTTCAGG GGTTGATATG AATGCTGCTA GGCTTTTATT CCACAAACTT 
ATACAACCTG ATCATCCTCA GATATCTCAG CAGGTGGCAG CTAGTTTGGA AAAGAATCTT 
ATTCCTAAAC TGACTAGTTC CTTACCTGAT GTTGAAGCAT TGAGGTTTTA TCTTACTCTA 
CCAGAATGTC CCCTGATGAG TGATTCCAAC AATTTCACAA CAATAGCAAT TCCCTTTGGT 
ACAGCTCTTG TAAATCTAGA AAAGGCACCA CTGAAAGTAC TTGAAAACTG GTGGTCAGTA 
CTTGAACCTC CACTATTCCT CAAGATTGTA GAACTTTTTA AGGAAGTTGT GGTACATCTT 
TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAAAGAA GAATTTTCAA CAGTTTTCTT 
CATACTGCAT TAAAGGTTTT AGAAATATTG CATAAGGTAA ATGAGAAAAC GGGACAGATT 
ATACAGTATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT AAGGAATGAT 
TATATCAACT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG 
TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA 
ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC 
AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT 
CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG 
AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA 
GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT 
GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA 
GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT 
ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC 
TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT 
TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC 
TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA 
CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT 
TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG 
CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG 
GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA 
ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT 
TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG 
TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT 
CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT 
AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417479.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273187.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe287519
Clone ID Related Accession (Same CDS sequence)	XM_022417479.1
Accession Version	XM_022417479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG 
GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA 
TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA 
GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC 
ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA 
GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA 
AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT 
CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC 
TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG 
ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273187.1
CDS	455..3616
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417479.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417479.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA 
TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG 
GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT 
GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA 
GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA 
GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA 
TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT 
TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA 
GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG 
CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC 
ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT 
ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA 
GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA 
TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT 
GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC 
ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA 
GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA 
AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT 
CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC 
TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG 
ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417482.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273190.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCe287520
Clone ID Related Accession (Same CDS sequence)	XM_022417482.1 , XM_022417480.1
Accession Version	XM_022417482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC 
AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273190.1
CDS	135..3230
Translation	MAAQVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417482.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417482.1

ORF Insert Sequence:

ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC 
AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417480.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273188.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OCe287520
Clone ID Related Accession (Same CDS sequence)	XM_022417482.1 , XM_022417480.1
Accession Version	XM_022417480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC 
AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273188.1
CDS	798..3893
Translation	MAAQVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417480.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417480.1

ORF Insert Sequence:

ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC 
AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_022417483.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022273191.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCe287521
Clone ID Related Accession (Same CDS sequence)	XM_022417483.1
Accession Version	XM_022417483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC 
AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022273191.1
CDS	926..4021
Translation	MEWIVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRSGGSIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_022417483.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022417483.1

ORF Insert Sequence:

ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC 
AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_014112839.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968314.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe35983
Clone ID Related Accession (Same CDS sequence)	XM_014112839.1 , XM_014112838.2 , XM_014112838.1
Accession Version	XM_014112839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA 
TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG 
CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT 
GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA 
ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT 
GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG 
AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT 
TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT 
GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG 
AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG 
AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT 
AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT 
GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT 
TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG 
GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT 
GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT 
TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA 
AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG 
AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC 
CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT 
AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968314.1
CDS	181..3468
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_014112839.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112839.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA 
TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG 
CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT 
GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA 
ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT 
GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG 
AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT 
TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT 
GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG 
AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG 
AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT 
AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT 
GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT 
TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG 
GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT 
GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT 
TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA 
AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG 
AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC 
CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT 
AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_014112838.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968313.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35983
Clone ID Related Accession (Same CDS sequence)	XM_014112839.1 , XM_014112838.2 , XM_014112838.1
Accession Version	XM_014112838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA 
TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG 
CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT 
GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA 
ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT 
GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG 
AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT 
TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT 
GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG 
AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG 
AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT 
AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT 
GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT 
TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG 
GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT 
GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT 
TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA 
AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG 
AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC 
CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT 
AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968313.1
CDS	455..3742
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_014112838.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112838.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA 
TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG 
CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT 
GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA 
ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT 
GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG 
AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT 
TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT 
GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG 
AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG 
AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT 
AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT 
GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT 
TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG 
GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT 
GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT 
TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA 
AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG 
AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC 
CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT 
AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_014112838.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968313.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

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CloneID	OCe35983
Clone ID Related Accession (Same CDS sequence)	XM_014112839.1 , XM_014112838.2 , XM_014112838.1
Accession Version	XM_014112838.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA 
TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG 
CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT 
GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA 
ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT 
GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG 
AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT 
TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT 
GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG 
AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG 
AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT 
AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT 
GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT 
TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG 
GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT 
GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT 
TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA 
AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG 
AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC 
CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT 
AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968313.1
CDS	465..3752
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_014112838.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112838.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA 
TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG 
CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT 
GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA 
ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT 
GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG 
AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT 
TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT 
GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG 
AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG 
AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT 
AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT 
GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT 
TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG 
GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT 
GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT 
TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA 
AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG 
AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC 
CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT 
AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_014112840.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968315.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe35984
Clone ID Related Accession (Same CDS sequence)	XM_014112840.1
Accession Version	XM_014112840.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA 
ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC 
AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT 
CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG 
AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA 
GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT 
GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA 
GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT 
ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC 
TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT 
TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC 
TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA 
CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT 
TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG 
CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG 
GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA 
ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT 
TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG 
TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT 
CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT 
AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968315.1
CDS	465..3728
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_014112840.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112840.1

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA 
CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT 
GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA 
CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG 
CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT 
TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA 
GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG 
AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC 
GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA 
ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA 
AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT 
GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA 
GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC 
TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG 
AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG 
AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA 
GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT 
GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT 
GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG 
GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA 
GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC 
ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA 
GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT 
TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA 
AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG 
GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA 
GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG 
TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA 
ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC 
AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT 
CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG 
AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA 
GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT 
GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA 
GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT 
ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC 
TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT 
TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC 
TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA 
CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT 
TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG 
CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG 
GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA 
ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT 
TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG 
TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT 
CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT 
AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_014112841.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968316.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe35985
Clone ID Related Accession (Same CDS sequence)	XM_014112841.1
Accession Version	XM_014112841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA 
AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT 
TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC 
ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC 
ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT 
GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT 
CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT 
CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT 
CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG 
GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA 
TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA 
AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT 
CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT 
CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT 
CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC 
TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC 
TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC 
ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA 
CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT 
AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA 
TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT 
CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA 
CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA 
CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT 
GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG 
TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC 
AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA 
GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT 
CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG 
AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT 
GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA 
GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA 
GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG 
TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT 
GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG 
GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT 
GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA 
GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT 
GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT 
CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG 
TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC 
CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG 
GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT 
TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA 
GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA 
GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA 
AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA 
GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968316.1
CDS	809..3886
Translation	MAAQVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_014112841.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112841.1

ORF Insert Sequence:

ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA 
AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT 
TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC 
ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC 
ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT 
GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT 
CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT 
CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT 
CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG 
GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA 
TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA 
AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT 
CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT 
CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT 
CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC 
TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC 
TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC 
ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA 
CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT 
AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA 
TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT 
CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA 
CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA 
CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT 
GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG 
TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC 
AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA 
GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT 
CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG 
AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT 
GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA 
GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA 
GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG 
TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT 
GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG 
GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT 
GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA 
GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT 
GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT 
CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG 
TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC 
CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG 
GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT 
TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA 
GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA 
GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA 
AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA 
GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_014112842.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013968317.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCe35986
Clone ID Related Accession (Same CDS sequence)	XM_014112842.1
Accession Version	XM_014112842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA 
AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT 
TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC 
ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC 
ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT 
GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT 
CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT 
CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT 
CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG 
GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA 
TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA 
AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT 
CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT 
CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT 
CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC 
TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC 
TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC 
ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA 
CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT 
AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA 
TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT 
CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA 
CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA 
CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT 
GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG 
TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC 
AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA 
GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT 
CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG 
AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT 
GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA 
GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA 
GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG 
TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT 
GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG 
GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT 
GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA 
GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT 
GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT 
CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG 
TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC 
CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG 
GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT 
TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA 
GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA 
GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA 
AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA 
GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013968317.1
CDS	90..3167
Translation	MEWIVEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_014112842.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014112842.1

ORF Insert Sequence:

ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC 
GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT 
AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT 
ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA 
AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT 
TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC 
ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC 
ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT 
GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT 
CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT 
CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT 
CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG 
GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA 
TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA 
AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT 
CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT 
CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT 
CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC 
TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC 
TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC 
ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA 
CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT 
AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA 
TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT 
CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA 
CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA 
CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT 
GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG 
TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC 
AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA 
GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT 
CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG 
AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT 
GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA 
GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA 
GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG 
TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT 
GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG 
GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT 
GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA 
GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT 
GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT 
CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG 
TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC 
CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG 
GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT 
TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA 
GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA 
GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA 
AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA 
GGCTTCAGTT TAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_005618934.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618991.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe04930
Clone ID Related Accession (Same CDS sequence)	XM_005618934.3 , XM_005618934.2
Accession Version	XM_005618934.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG 
GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618991.1
CDS	454..3699
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_005618934.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618934.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG 
GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_005618934.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618991.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe04930
Clone ID Related Accession (Same CDS sequence)	XM_005618934.3 , XM_005618934.2
Accession Version	XM_005618934.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG 
GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618991.1
CDS	465..3710
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSCLPKIMGIDTLVRTCSGLSYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_005618934.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618934.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG 
GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT 
ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT 
GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA 
AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA 
GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC 
AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA 
AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA 
CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT 
AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT 
ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT 
TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA 
AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT 
GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA 
CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA 
AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG 
AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA 
AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA 
GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT 
CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT 
TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG 
AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA 
GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC 
CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC 
GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT 
TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT 
TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA 
CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT 
ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA 
GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT 
CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA 
AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG 
CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA 
TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT 
GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT 
TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT 
CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA 
CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT 
GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG 
AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT 
GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT 
CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA 
CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA 
GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT 
CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA 
ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG 
GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA 
AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_005618937.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618994.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe04932
Clone ID Related Accession (Same CDS sequence)	XM_005618937.3 , XM_005618937.2
Accession Version	XM_005618937.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618994.1
CDS	454..3669
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_005618937.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618937.3

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_005618937.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005618994.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

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CloneID	OCe04932
Clone ID Related Accession (Same CDS sequence)	XM_005618937.3 , XM_005618937.2
Accession Version	XM_005618937.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005618994.1
CDS	464..3679
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPSNSYRDKRELSRETRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_005618937.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005618937.2

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA 
GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC 
TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT 
GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT 
TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC 
CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT 
GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT 
ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT 
GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG 
AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT 
TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG 
CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC 
CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA 
ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA 
CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA 
ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA 
GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT 
GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA 
TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG 
GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT 
TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA 
ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC 
TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT 
GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC 
AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA 
ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC 
ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT 
GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT 
GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT 
GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT 
CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT 
AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT 
GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG 
GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT 
AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT 
TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA 
AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG 
CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC 
ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA 
GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT 
AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA 
AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT 
TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT 
CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA 
TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA 
CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT 
AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA 
GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_844715.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849808.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OCe04933
Clone ID Related Accession (Same CDS sequence)	XM_844715.5 , XM_844715.4
Accession Version	XM_844715.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_844715.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC 
ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT 
ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA 
TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT 
GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG 
GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA 
TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT 
GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA 
GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA 
CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849808.1
CDS	454..3627
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_844715.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844715.5

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC 
ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT 
ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA 
TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT 
GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG 
GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA 
TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT 
GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA 
GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA 
CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_844715.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_849808.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe04933
Clone ID Related Accession (Same CDS sequence)	XM_844715.5 , XM_844715.4
Accession Version	XM_844715.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844715.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC 
ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT 
ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA 
TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT 
GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG 
GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA 
TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT 
GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA 
GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA 
CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_849808.1
CDS	464..3637
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_844715.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_844715.4

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA 
TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA 
CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA 
GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA 
GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT 
ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC 
ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG 
CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT 
ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA 
TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT 
GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT 
TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT 
CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT 
GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT 
CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG 
GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG 
GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA 
TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT 
GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA 
GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA 
CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_536367.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536367.2 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OCe04934
Clone ID Related Accession (Same CDS sequence)	XM_536367.5 , XM_536367.6
Accession Version	XM_536367.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536367.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG 
GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT 
GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT 
AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT 
TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA 
CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC 
CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG 
ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG 
TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC 
ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT 
CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT 
GATCATAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536367.2
CDS	463..3612
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_536367.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536367.5

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG 
GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT 
GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT 
AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT 
TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA 
CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC 
CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG 
ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG 
TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC 
ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT 
CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT 
GATCATAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HERC4 ( XM_536367.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536367.2 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OCe04934
Clone ID Related Accession (Same CDS sequence)	XM_536367.5 , XM_536367.6
Accession Version	XM_536367.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	probable E3 ubiquitin-protein ligase HERC4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536367.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG 
GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT 
GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT 
AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT 
TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA 
CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC 
CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG 
ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG 
TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC 
ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT 
CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT 
GATCATAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536367.2
CDS	453..3602
Translation	MLCWGNASFGQLGLGGIDEEIVLEPRKSDFFINKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEKSRKKPEQVVALDAQNIVAVSCGEAHTLALNDKGQVYAWGLDSDGQLGLLGSEECIRVPRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYVPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPPDIDSEEYFCVKRIFSGGDQSFSHYSNPQNCGPPDDFRCPDPSKQIWTVNETLIQKWLSYPSGRFPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYRTGTKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTSSLPDVEALRFYLTLPECPLMSDSNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIVELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHKVNEKTGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELVLNGADTAVNKQNRQEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLVIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI

Target ORF information:

RefSeq Version	XM_536367.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536367.6

ORF Insert Sequence:

ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA 
TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT 
AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC 
CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT 
GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA 
TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA 
CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT 
TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA 
CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC 
CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT 
CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA 
ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA 
GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT 
CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT 
TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA 
CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT 
GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA 
TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT 
CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG 
ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG 
GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT 
CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG 
AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG 
CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT 
GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC 
AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA 
CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA 
GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT 
TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG 
CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA 
GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT 
GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA 
GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG 
GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC 
TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA 
ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT 
GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT 
AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA 
TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT 
TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG 
CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA 
CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT 
GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT 
AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA 
GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC 
CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT 
TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG 
ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG 
TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC 
ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT 
CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT 
GATCATAATG AAGGCTTCAG TTTAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	HERC4
Entrez Gene ID	479225
Full Name	HECT and RLD domain containing E3 ubiquitin protein ligase 4
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	HERC4	NP_071362.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006994	XP_308776.4
Canis lupus familiaris (dog)	HERC4	XP_005618991.1
Danio rerio (zebrafish)	herc4	XP_685685.5
Drosophila melanogaster (fruit fly)	CG9153	NP_001261249.1
Bos taurus (cattle)	HERC4	NP_001070362.2
Pan troglodytes (chimpanzee)	HERC4	XP_001167753.1
Mus musculus (house mouse)	Herc4	NP_084390.1
Rattus norvegicus (Norway rat)	Herc4	NP_001012074.1
Gallus gallus (chicken)	HERC4	XP_004942077.1
Arabidopsis thaliana (thale cress)	UVR8	NP_201191.1
Oryza sativa (rice)	Os02g0554100	NP_001047115.1
Xenopus tropicalis (tropical clawed frog)	herc4	NP_001122122.1
	HERC4	XP_001087608.1
Oryza sativa (rice)	Os04g0435700	NP_001052849.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

HERC4 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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