HERC4 cDNA ORF clone, Canis lupus familiaris(dog)

The following HERC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HERC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe04937 XM_022417484.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe287516 XM_022417474.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287516 XM_022417476.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287517 XM_022417477.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287518 XM_022417478.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287519 XM_022417479.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287520 XM_022417480.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287520 XM_022417482.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe287521 XM_022417483.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35983 XM_014112838.2
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35983 XM_014112839.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35984 XM_014112840.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35985 XM_014112841.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe35986 XM_014112842.1
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe04930 XM_005618934.3
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe04932 XM_005618937.3
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe04933 XM_844715.5
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe04934 XM_536367.6
Latest version!
Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe04933 XM_844715.4 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe04932 XM_005618937.2 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe35983 XM_014112838.1 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe04930 XM_005618934.2 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe04934 XM_536367.5 Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe04937
    Clone ID Related Accession (Same CDS sequence) XM_022417484.1
    Accession Version XM_022417484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAAA TGGAAGGTGG CGTCTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGT 
    CATAACTCTA CCAGTCATGA AATAAACCCA CGGAAAGTTT TTGAACTTAT GGGAAGCATT
    GTTACTCAGA TTGCTTGTGG AAGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA
    ATTTATTCTT TTGGACTTGG TGGTAATGGG CAGCTAGGAA CTGGTTCAAC AAGCAACAGG
    AAAAGTCCTT TTACTGTGAA AGGAAATTGG TTCCCTTATA ATGGGCAGTG TCCACCAGAT
    ATTGATTCTG AAGAATATTT CTGTGTGAAA AGAATTTTCT CAGGTGGAGA TCAAAGCTTT
    TCACATTACT CTAATCCCCA GAACTGTGGG CCACCAGATG ACTTTAGATG TCCTGATCCT
    TCAAAGCAGA TCTGGACTGT GAATGAAACT CTAATTCAAA AATGGCTGAG CTACCCATCT
    GGGAGGTTTC CTGTGGAGAT AGCCAATGAA ATAGATGGAA CATTTTCTTC CTCTGGCTGT
    CTAAATGGAA GTTTTCTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG CACCAAGTTT
    TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT
    CCTCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT
    AGTTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG
    ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTAAAT
    CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA
    TTCCTCAAGA TTGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG
    ATCGGCATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG
    GTTTTAGAAA TATTGCATAA GGTAAATGAG AAAACGGGAC AGATTATACA GTATGATAAA
    TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGGA ATGATTATAT CAACTGGGTC
    CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGCAC ATATCCATTT
    GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAAACAG ATGCAGTCTT ACAGATGCAG
    ATGGCTATTG ACCAGGCCCA CAGACAGAAT GTCTCCTCTC TTTTCCTCCC AGTGATTGAA
    TCTGTGAATC CCTGCTTAAT TCTAGTTGTA CGTAGAGAAA ATATTGTAGG AGATGCAATG
    GAAGTCCTTA GGAAAACAAA GAATATAGAC TACAAAAAGC CACTCAAGGT TATATTTGTT
    GGAGAAGATG CTGTTGATGC AGGAGGAGTA CGCAAAGAAT TTTTCTTGCT CATCATGAGA
    GAATTGTTGG ATCCTAAATA TGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG
    TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTTCATT TGATTGGTGT TATCTGTGGA
    TTAGCAATTT ATAATTTTAC TATTGTGGAC CTCCACTTTC CTTTGGCTTT ATATAAGAAA
    CTGCTGAAAA AGAAGCCATC CTTGGATGAT TTGAAGGAAC TAGTGCCTGA TGTTGGGAGA
    AGCATGCAGC AATTACTGGA TTATCCAGAA GATGATGTTG AGGAAACATT TTGTCTTAAT
    TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT
    GCAGACACAG CTGTTAACAA ACAAAATCGG CAGGAGTTTG TTGATGCTTA TGTGGATTAC
    ATATTCAATA AATCAGTGGC TTCCTTGTTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC
    TGTGGAGGAA AGGTCCTTCA GCTCTTCCAG CCAAATGAAC TACAAGCAAT GGTTATTGGA
    AACACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAGTATTGG
    GCAGAGCATC CAACGATAAA AATTTTTTGG GAAGTATTTC ATGAATTACC ATTGGAAAAG
    AAAAAACAAT TTCTGTTATT TTTGACAGGT AGTGATCGAA TACCTATTCT TGGTATGAAG
    AGTCTAAAAC TAGTCATCCA GTCAACAGGA GGTGGTGAGG AATATCTCCC AGTTTCCCAT
    ACCTGTTTTA ATCTTCTGGA TCTTCCAAAA TACACCGAAA AAGAAACTCT ACGCTCTAAG
    CTGATTCAAG CAATTGATCA TAATGAAGGC TTCAGTTTAA TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273192.1
    CDS145..2529
    Translation

    Target ORF information:

    RefSeq Version XM_022417484.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGATGAAAA TGGAAGGTGG CGTCTTTACT TTTGGAGCTG GAGGGTATGG TCAGTTGGGT 
    CATAACTCTA CCAGTCATGA AATAAACCCA CGGAAAGTTT TTGAACTTAT GGGAAGCATT
    GTTACTCAGA TTGCTTGTGG AAGGCAGCAC ACTTCTGCTT TTGTTCCTTC ATCAGGACGA
    ATTTATTCTT TTGGACTTGG TGGTAATGGG CAGCTAGGAA CTGGTTCAAC AAGCAACAGG
    AAAAGTCCTT TTACTGTGAA AGGAAATTGG TTCCCTTATA ATGGGCAGTG TCCACCAGAT
    ATTGATTCTG AAGAATATTT CTGTGTGAAA AGAATTTTCT CAGGTGGAGA TCAAAGCTTT
    TCACATTACT CTAATCCCCA GAACTGTGGG CCACCAGATG ACTTTAGATG TCCTGATCCT
    TCAAAGCAGA TCTGGACTGT GAATGAAACT CTAATTCAAA AATGGCTGAG CTACCCATCT
    GGGAGGTTTC CTGTGGAGAT AGCCAATGAA ATAGATGGAA CATTTTCTTC CTCTGGCTGT
    CTAAATGGAA GTTTTCTAGC TGTTAGCAAT GATGATCACT ATAGAACAGG CACCAAGTTT
    TCAGGGGTTG ATATGAATGC TGCTAGGCTT TTATTCCACA AACTTATACA ACCTGATCAT
    CCTCAGATAT CTCAGCAGGT GGCAGCTAGT TTGGAAAAGA ATCTTATTCC TAAACTGACT
    AGTTCCTTAC CTGATGTTGA AGCATTGAGG TTTTATCTTA CTCTACCAGA ATGTCCCCTG
    ATGAGTGATT CCAACAATTT CACAACAATA GCAATTCCCT TTGGTACAGC TCTTGTAAAT
    CTAGAAAAGG CACCACTGAA AGTACTTGAA AACTGGTGGT CAGTACTTGA ACCTCCACTA
    TTCCTCAAGA TTGTAGAACT TTTTAAGGAA GTTGTGGTAC ATCTTTTGAA ACTCTACAAG
    ATCGGCATTC CCCCTTCTGA AAGAAGAATT TTCAACAGTT TTCTTCATAC TGCATTAAAG
    GTTTTAGAAA TATTGCATAA GGTAAATGAG AAAACGGGAC AGATTATACA GTATGATAAA
    TTTTATATAC ATGAAGTACA AGAATTGATA GACATAAGGA ATGATTATAT CAACTGGGTC
    CAACAGCAGG CCTATGGAAT GTTGGCAGAT ATCCCTGTTA CAATCTGCAC ATATCCATTT
    GTATTTGATG CCCAAGCAAA AACTACTCTG TTACAAACAG ATGCAGTCTT ACAGATGCAG
    ATGGCTATTG ACCAGGCCCA CAGACAGAAT GTCTCCTCTC TTTTCCTCCC AGTGATTGAA
    TCTGTGAATC CCTGCTTAAT TCTAGTTGTA CGTAGAGAAA ATATTGTAGG AGATGCAATG
    GAAGTCCTTA GGAAAACAAA GAATATAGAC TACAAAAAGC CACTCAAGGT TATATTTGTT
    GGAGAAGATG CTGTTGATGC AGGAGGAGTA CGCAAAGAAT TTTTCTTGCT CATCATGAGA
    GAATTGTTGG ATCCTAAATA TGGCATGTTT AGGTATTATG AAGATTCCAG GCTCATTTGG
    TTTTCTGATA AGACATTTGA AGACAGTGAT TTGTTTCATT TGATTGGTGT TATCTGTGGA
    TTAGCAATTT ATAATTTTAC TATTGTGGAC CTCCACTTTC CTTTGGCTTT ATATAAGAAA
    CTGCTGAAAA AGAAGCCATC CTTGGATGAT TTGAAGGAAC TAGTGCCTGA TGTTGGGAGA
    AGCATGCAGC AATTACTGGA TTATCCAGAA GATGATGTTG AGGAAACATT TTGTCTTAAT
    TTTACGATCA CAGTTGAAAA CTTTGGTGCA ACAGAAGTGA AAGAGCTGGT TCTAAATGGT
    GCAGACACAG CTGTTAACAA ACAAAATCGG CAGGAGTTTG TTGATGCTTA TGTGGATTAC
    ATATTCAATA AATCAGTGGC TTCCTTGTTT GATGCTTTTC ATGCGGGCTT TCATAAGGTC
    TGTGGAGGAA AGGTCCTTCA GCTCTTCCAG CCAAATGAAC TACAAGCAAT GGTTATTGGA
    AACACAAATT ATGATTGGAA GGAACTGGAA AAGAATACAG AATACAAAGG GGAGTATTGG
    GCAGAGCATC CAACGATAAA AATTTTTTGG GAAGTATTTC ATGAATTACC ATTGGAAAAG
    AAAAAACAAT TTCTGTTATT TTTGACAGGT AGTGATCGAA TACCTATTCT TGGTATGAAG
    AGTCTAAAAC TAGTCATCCA GTCAACAGGA GGTGGTGAGG AATATCTCCC AGTTTCCCAT
    ACCTGTTTTA ATCTTCTGGA TCTTCCAAAA TACACCGAAA AAGAAACTCT ACGCTCTAAG
    CTGATTCAAG CAATTGATCA TAATGAAGGC TTCAGTTTAA TATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287516
    Clone ID Related Accession (Same CDS sequence) XM_022417474.1 , XM_022417476.1
    Accession Version XM_022417474.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273182.1
    CDS455..3760
    Translation

    Target ORF information:

    RefSeq Version XM_022417474.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417474.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287516
    Clone ID Related Accession (Same CDS sequence) XM_022417474.1 , XM_022417476.1
    Accession Version XM_022417476.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273184.1
    CDS164..3469
    Translation

    Target ORF information:

    RefSeq Version XM_022417476.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417476.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287517
    Clone ID Related Accession (Same CDS sequence) XM_022417477.1
    Accession Version XM_022417477.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA
    TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG
    GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT
    GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA
    GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA
    GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA
    TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT
    TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA
    GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG
    CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC
    ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT
    ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA
    GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA
    TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT
    GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC
    ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA
    GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA
    AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT
    CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC
    TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG
    ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273185.1
    CDS454..3735
    Translation

    Target ORF information:

    RefSeq Version XM_022417477.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417477.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA
    TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG
    GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT
    GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA
    GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA
    GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA
    TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT
    TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA
    GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG
    CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC
    ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT
    ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA
    GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA
    TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT
    GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC
    ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA
    GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA
    AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT
    CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC
    TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG
    ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287518
    Clone ID Related Accession (Same CDS sequence) XM_022417478.1
    Accession Version XM_022417478.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG
    GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGGT CAGGTGGCAG TATCAGAAAT
    ATTAAAAGTT TGTCAGATAT CCAGATAGTA CAGGTTGCTT GCGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCGAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAGTTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT GCTTGGAATT
    CCTTTCATGC AAGTTGCAGC AGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATTTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCAGCACT AACCAAGGAA GGTGGCGTCT TTACTTTTGG AGCTGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCACGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTTAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGGCAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTTACT GTGAAAGGAA ATTGGTTCCC TTATAATGGG
    CAGTGTCCAC CAGATATTGA TTCTGAAGAA TATTTCTGTG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTACTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATGTCCTG ATCCTTCAAA GCAGATCTGG ACTGTGAATG AAACTCTAAT TCAAAAATGG
    CTGAGCTACC CATCTGGGAG GTTTCCTGTG GAGATAGCCA ATGAAATAGA TGGAACATTT
    TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT CTAGCTGTTA GCAATGATGA TCACTATAGA
    ACAGGCACCA AGTTTTCAGG GGTTGATATG AATGCTGCTA GGCTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATATCTCAG CAGGTGGCAG CTAGTTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGTTC CTTACCTGAT GTTGAAGCAT TGAGGTTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG TGATTCCAAC AATTTCACAA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TAAATCTAGA AAAGGCACCA CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CACTATTCCT CAAGATTGTA GAACTTTTTA AGGAAGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAAAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTG CATAAGGTAA ATGAGAAAAC GGGACAGATT
    ATACAGTATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT AAGGAATGAT
    TATATCAACT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT
    CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT
    GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC
    TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT
    TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG
    CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA
    ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT
    TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273186.1
    CDS455..3718
    Translation

    Target ORF information:

    RefSeq Version XM_022417478.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417478.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG
    GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGGT CAGGTGGCAG TATCAGAAAT
    ATTAAAAGTT TGTCAGATAT CCAGATAGTA CAGGTTGCTT GCGGTTACTA TCATTCACTT
    GCACTTTCTA AAGCGAGTGA AGTCTTCTGT TGGGGACAGA ATAAATATGG CCAGTTGGGT
    TTAGGCATTG ACTGTAAAAA GCAAGCTTCA CCACAGCTGA TTAAGTCTTT GCTTGGAATT
    CCTTTCATGC AAGTTGCAGC AGGAGGAGCC CATAGTTTTG TACTCACCCT TTCTGGAGCT
    ATTTTTGGAT GGGGACGCAA CAAATTTGGT CAACTAGGTC TTAATGATGA AAATGATAGG
    TATGTTCCTA ATTTACTAAA GTCACTAAGA ACTCAGAAAA TAGTTTATAT TTGTTGTGGA
    GAAGATCATA CTGCAGCACT AACCAAGGAA GGTGGCGTCT TTACTTTTGG AGCTGGAGGG
    TATGGTCAGT TGGGTCATAA CTCTACCAGT CATGAAATAA ACCCACGGAA AGTTTTTGAA
    CTTATGGGAA GCATTGTTAC TCAGATTGCT TGTGGAAGGC AGCACACTTC TGCTTTTGTT
    CCTTCATCAG GACGAATTTA TTCTTTTGGA CTTGGTGGTA ATGGGCAGCT AGGAACTGGT
    TCAACAAGCA ACAGGAAAAG TCCTTTTACT GTGAAAGGAA ATTGGTTCCC TTATAATGGG
    CAGTGTCCAC CAGATATTGA TTCTGAAGAA TATTTCTGTG TGAAAAGAAT TTTCTCAGGT
    GGAGATCAAA GCTTTTCACA TTACTCTAAT CCCCAGAACT GTGGGCCACC AGATGACTTT
    AGATGTCCTG ATCCTTCAAA GCAGATCTGG ACTGTGAATG AAACTCTAAT TCAAAAATGG
    CTGAGCTACC CATCTGGGAG GTTTCCTGTG GAGATAGCCA ATGAAATAGA TGGAACATTT
    TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT CTAGCTGTTA GCAATGATGA TCACTATAGA
    ACAGGCACCA AGTTTTCAGG GGTTGATATG AATGCTGCTA GGCTTTTATT CCACAAACTT
    ATACAACCTG ATCATCCTCA GATATCTCAG CAGGTGGCAG CTAGTTTGGA AAAGAATCTT
    ATTCCTAAAC TGACTAGTTC CTTACCTGAT GTTGAAGCAT TGAGGTTTTA TCTTACTCTA
    CCAGAATGTC CCCTGATGAG TGATTCCAAC AATTTCACAA CAATAGCAAT TCCCTTTGGT
    ACAGCTCTTG TAAATCTAGA AAAGGCACCA CTGAAAGTAC TTGAAAACTG GTGGTCAGTA
    CTTGAACCTC CACTATTCCT CAAGATTGTA GAACTTTTTA AGGAAGTTGT GGTACATCTT
    TTGAAACTCT ACAAGATCGG CATTCCCCCT TCTGAAAGAA GAATTTTCAA CAGTTTTCTT
    CATACTGCAT TAAAGGTTTT AGAAATATTG CATAAGGTAA ATGAGAAAAC GGGACAGATT
    ATACAGTATG ATAAATTTTA TATACATGAA GTACAAGAAT TGATAGACAT AAGGAATGAT
    TATATCAACT GGGTCCAACA GCAGGCCTAT GGAATGGATG TCAACCATGG ATTAACTGAG
    TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT
    CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT
    GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC
    TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT
    TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG
    CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA
    ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT
    TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287519
    Clone ID Related Accession (Same CDS sequence) XM_022417479.1
    Accession Version XM_022417479.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA
    TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG
    GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT
    GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA
    GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA
    GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA
    TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT
    TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA
    GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG
    CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC
    ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT
    ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA
    GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA
    TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT
    GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC
    ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA
    GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA
    AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT
    CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC
    TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG
    ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273187.1
    CDS455..3616
    Translation

    Target ORF information:

    RefSeq Version XM_022417479.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417479.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG GTCAGGTGGC AGTATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGTT GGCAGATATC CCTGTTACAA TCTGCACATA TCCATTTGTA
    TTTGATGCCC AAGCAAAAAC TACTCTGTTA CAAACAGATG CAGTCTTACA GATGCAGATG
    GCTATTGACC AGGCCCACAG ACAGAATGTC TCCTCTCTTT TCCTCCCAGT GATTGAATCT
    GTGAATCCCT GCTTAATTCT AGTTGTACGT AGAGAAAATA TTGTAGGAGA TGCAATGGAA
    GTCCTTAGGA AAACAAAGAA TATAGACTAC AAAAAGCCAC TCAAGGTTAT ATTTGTTGGA
    GAAGATGCTG TTGATGCAGG AGGAGTACGC AAAGAATTTT TCTTGCTCAT CATGAGAGAA
    TTGTTGGATC CTAAATATGG CATGTTTAGG TATTATGAAG ATTCCAGGCT CATTTGGTTT
    TCTGATAAGA CATTTGAAGA CAGTGATTTG TTTCATTTGA TTGGTGTTAT CTGTGGATTA
    GCAATTTATA ATTTTACTAT TGTGGACCTC CACTTTCCTT TGGCTTTATA TAAGAAACTG
    CTGAAAAAGA AGCCATCCTT GGATGATTTG AAGGAACTAG TGCCTGATGT TGGGAGAAGC
    ATGCAGCAAT TACTGGATTA TCCAGAAGAT GATGTTGAGG AAACATTTTG TCTTAATTTT
    ACGATCACAG TTGAAAACTT TGGTGCAACA GAAGTGAAAG AGCTGGTTCT AAATGGTGCA
    GACACAGCTG TTAACAAACA AAATCGGCAG GAGTTTGTTG ATGCTTATGT GGATTACATA
    TTCAATAAAT CAGTGGCTTC CTTGTTTGAT GCTTTTCATG CGGGCTTTCA TAAGGTCTGT
    GGAGGAAAGG TCCTTCAGCT CTTCCAGCCA AATGAACTAC AAGCAATGGT TATTGGAAAC
    ACAAATTATG ATTGGAAGGA ACTGGAAAAG AATACAGAAT ACAAAGGGGA GTATTGGGCA
    GAGCATCCAA CGATAAAAAT TTTTTGGGAA GTATTTCATG AATTACCATT GGAAAAGAAA
    AAACAATTTC TGTTATTTTT GACAGGTAGT GATCGAATAC CTATTCTTGG TATGAAGAGT
    CTAAAACTAG TCATCCAGTC AACAGGAGGT GGTGAGGAAT ATCTCCCAGT TTCCCATACC
    TGTTTTAATC TTCTGGATCT TCCAAAATAC ACCGAAAAAG AAACTCTACG CTCTAAGCTG
    ATTCAAGCAA TTGATCATAA TGAAGGCTTC AGTTTAATAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287520
    Clone ID Related Accession (Same CDS sequence) XM_022417482.1 , XM_022417480.1
    Accession Version XM_022417482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC
    AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273190.1
    CDS135..3230
    Translation

    Target ORF information:

    RefSeq Version XM_022417482.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC
    AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287520
    Clone ID Related Accession (Same CDS sequence) XM_022417482.1 , XM_022417480.1
    Accession Version XM_022417480.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC
    AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273188.1
    CDS798..3893
    Translation

    Target ORF information:

    RefSeq Version XM_022417480.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417480.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC
    AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287521
    Clone ID Related Accession (Same CDS sequence) XM_022417483.1
    Accession Version XM_022417483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3096bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC
    AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273191.1
    CDS926..4021
    Translation

    Target ORF information:

    RefSeq Version XM_022417483.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG GTCAGGTGGC
    AGTATCAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35983
    Clone ID Related Accession (Same CDS sequence) XM_014112839.1 , XM_014112838.2 , XM_014112838.1
    Accession Version XM_014112839.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA
    TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG
    CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT
    GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA
    ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT
    GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG
    AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT
    TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT
    GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG
    AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG
    AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT
    AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT
    GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT
    TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG
    GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT
    GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT
    TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA
    AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG
    AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC
    CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT
    AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968314.1
    CDS181..3468
    Translation

    Target ORF information:

    RefSeq Version XM_014112839.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112839.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA
    TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG
    CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT
    GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA
    ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT
    GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG
    AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT
    TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT
    GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG
    AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG
    AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT
    AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT
    GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT
    TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG
    GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT
    GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT
    TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA
    AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG
    AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC
    CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT
    AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35983
    Clone ID Related Accession (Same CDS sequence) XM_014112839.1 , XM_014112838.2 , XM_014112838.1
    Accession Version XM_014112838.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014112838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA
    TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG
    CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT
    GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA
    ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT
    GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG
    AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT
    TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT
    GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG
    AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG
    AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT
    AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT
    GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT
    TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG
    GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT
    GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT
    TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA
    AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG
    AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC
    CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT
    AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968313.1
    CDS455..3742
    Translation

    Target ORF information:

    RefSeq Version XM_014112838.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112838.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA
    TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG
    CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT
    GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA
    ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT
    GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG
    AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT
    TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT
    GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG
    AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG
    AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT
    AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT
    GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT
    TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG
    GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT
    GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT
    TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA
    AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG
    AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC
    CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT
    AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35983
    Clone ID Related Accession (Same CDS sequence) XM_014112839.1 , XM_014112838.2 , XM_014112838.1
    Accession Version XM_014112838.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA
    TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG
    CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT
    GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA
    ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT
    GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG
    AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT
    TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT
    GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG
    AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG
    AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT
    AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT
    GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT
    TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG
    GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT
    GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT
    TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA
    AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG
    AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC
    CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT
    AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968313.1
    CDS465..3752
    Translation

    Target ORF information:

    RefSeq Version XM_014112838.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112838.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    GATGTCAACC ATGGATTAAC TGAGTTGGCA GATATCCCTG TTACAATCTG CACATATCCA
    TTTGTATTTG ATGCCCAAGC AAAAACTACT CTGTTACAAA CAGATGCAGT CTTACAGATG
    CAGATGGCTA TTGACCAGGC CCACAGACAG AATGTCTCCT CTCTTTTCCT CCCAGTGATT
    GAATCTGTGA ATCCCTGCTT AATTCTAGTT GTACGTAGAG AAAATATTGT AGGAGATGCA
    ATGGAAGTCC TTAGGAAAAC AAAGAATATA GACTACAAAA AGCCACTCAA GGTTATATTT
    GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA GTACGCAAAG AATTTTTCTT GCTCATCATG
    AGAGAATTGT TGGATCCTAA ATATGGCATG TTTAGGTATT ATGAAGATTC CAGGCTCATT
    TGGTTTTCTG ATAAGACATT TGAAGACAGT GATTTGTTTC ATTTGATTGG TGTTATCTGT
    GGATTAGCAA TTTATAATTT TACTATTGTG GACCTCCACT TTCCTTTGGC TTTATATAAG
    AAACTGCTGA AAAAGAAGCC ATCCTTGGAT GATTTGAAGG AACTAGTGCC TGATGTTGGG
    AGAAGCATGC AGCAATTACT GGATTATCCA GAAGATGATG TTGAGGAAAC ATTTTGTCTT
    AATTTTACGA TCACAGTTGA AAACTTTGGT GCAACAGAAG TGAAAGAGCT GGTTCTAAAT
    GGTGCAGACA CAGCTGTTAA CAAACAAAAT CGGCAGGAGT TTGTTGATGC TTATGTGGAT
    TACATATTCA ATAAATCAGT GGCTTCCTTG TTTGATGCTT TTCATGCGGG CTTTCATAAG
    GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC CAGCCAAATG AACTACAAGC AATGGTTATT
    GGAAACACAA ATTATGATTG GAAGGAACTG GAAAAGAATA CAGAATACAA AGGGGAGTAT
    TGGGCAGAGC ATCCAACGAT AAAAATTTTT TGGGAAGTAT TTCATGAATT ACCATTGGAA
    AAGAAAAAAC AATTTCTGTT ATTTTTGACA GGTAGTGATC GAATACCTAT TCTTGGTATG
    AAGAGTCTAA AACTAGTCAT CCAGTCAACA GGAGGTGGTG AGGAATATCT CCCAGTTTCC
    CATACCTGTT TTAATCTTCT GGATCTTCCA AAATACACCG AAAAAGAAAC TCTACGCTCT
    AAGCTGATTC AAGCAATTGA TCATAATGAA GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35984
    Clone ID Related Accession (Same CDS sequence) XM_014112840.1
    Accession Version XM_014112840.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3264bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT
    CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT
    GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC
    TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT
    TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG
    CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA
    ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT
    TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968315.1
    CDS465..3728
    Translation

    Target ORF information:

    RefSeq Version XM_014112840.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112840.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGCT GCCTCCCGAA AATCATGGGT ATCGACACTC TGGTCAGAAC TTGCTCCGGA
    CTCTCATATG GCAGAATCAG AAATATTAAA AGTTTGTCAG ATATCCAGAT AGTACAGGTT
    GCTTGCGGTT ACTATCATTC ACTTGCACTT TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA
    CAGAATAAAT ATGGCCAGTT GGGTTTAGGC ATTGACTGTA AAAAGCAAGC TTCACCACAG
    CTGATTAAGT CTTTGCTTGG AATTCCTTTC ATGCAAGTTG CAGCAGGAGG AGCCCATAGT
    TTTGTACTCA CCCTTTCTGG AGCTATTTTT GGATGGGGAC GCAACAAATT TGGTCAACTA
    GGTCTTAATG ATGAAAATGA TAGGTATGTT CCTAATTTAC TAAAGTCACT AAGAACTCAG
    AAAATAGTTT ATATTTGTTG TGGAGAAGAT CATACTGCAG CACTAACCAA GGAAGGTGGC
    GTCTTTACTT TTGGAGCTGG AGGGTATGGT CAGTTGGGTC ATAACTCTAC CAGTCATGAA
    ATAAACCCAC GGAAAGTTTT TGAACTTATG GGAAGCATTG TTACTCAGAT TGCTTGTGGA
    AGGCAGCACA CTTCTGCTTT TGTTCCTTCA TCAGGACGAA TTTATTCTTT TGGACTTGGT
    GGTAATGGGC AGCTAGGAAC TGGTTCAACA AGCAACAGGA AAAGTCCTTT TACTGTGAAA
    GGAAATTGGT TCCCTTATAA TGGGCAGTGT CCACCAGATA TTGATTCTGA AGAATATTTC
    TGTGTGAAAA GAATTTTCTC AGGTGGAGAT CAAAGCTTTT CACATTACTC TAATCCCCAG
    AACTGTGGGC CACCAGATGA CTTTAGATGT CCTGATCCTT CAAAGCAGAT CTGGACTGTG
    AATGAAACTC TAATTCAAAA ATGGCTGAGC TACCCATCTG GGAGGTTTCC TGTGGAGATA
    GCCAATGAAA TAGATGGAAC ATTTTCTTCC TCTGGCTGTC TAAATGGAAG TTTTCTAGCT
    GTTAGCAATG ATGATCACTA TAGAACAGGC ACCAAGTTTT CAGGGGTTGA TATGAATGCT
    GCTAGGCTTT TATTCCACAA ACTTATACAA CCTGATCATC CTCAGATATC TCAGCAGGTG
    GCAGCTAGTT TGGAAAAGAA TCTTATTCCT AAACTGACTA GTTCCTTACC TGATGTTGAA
    GCATTGAGGT TTTATCTTAC TCTACCAGAA TGTCCCCTGA TGAGTGATTC CAACAATTTC
    ACAACAATAG CAATTCCCTT TGGTACAGCT CTTGTAAATC TAGAAAAGGC ACCACTGAAA
    GTACTTGAAA ACTGGTGGTC AGTACTTGAA CCTCCACTAT TCCTCAAGAT TGTAGAACTT
    TTTAAGGAAG TTGTGGTACA TCTTTTGAAA CTCTACAAGA TCGGCATTCC CCCTTCTGAA
    AGAAGAATTT TCAACAGTTT TCTTCATACT GCATTAAAGG TTTTAGAAAT ATTGCATAAG
    GTAAATGAGA AAACGGGACA GATTATACAG TATGATAAAT TTTATATACA TGAAGTACAA
    GAATTGATAG ACATAAGGAA TGATTATATC AACTGGGTCC AACAGCAGGC CTATGGAATG
    TTGGCAGATA TCCCTGTTAC AATCTGCACA TATCCATTTG TATTTGATGC CCAAGCAAAA
    ACTACTCTGT TACAAACAGA TGCAGTCTTA CAGATGCAGA TGGCTATTGA CCAGGCCCAC
    AGACAGAATG TCTCCTCTCT TTTCCTCCCA GTGATTGAAT CTGTGAATCC CTGCTTAATT
    CTAGTTGTAC GTAGAGAAAA TATTGTAGGA GATGCAATGG AAGTCCTTAG GAAAACAAAG
    AATATAGACT ACAAAAAGCC ACTCAAGGTT ATATTTGTTG GAGAAGATGC TGTTGATGCA
    GGAGGAGTAC GCAAAGAATT TTTCTTGCTC ATCATGAGAG AATTGTTGGA TCCTAAATAT
    GGCATGTTTA GGTATTATGA AGATTCCAGG CTCATTTGGT TTTCTGATAA GACATTTGAA
    GACAGTGATT TGTTTCATTT GATTGGTGTT ATCTGTGGAT TAGCAATTTA TAATTTTACT
    ATTGTGGACC TCCACTTTCC TTTGGCTTTA TATAAGAAAC TGCTGAAAAA GAAGCCATCC
    TTGGATGATT TGAAGGAACT AGTGCCTGAT GTTGGGAGAA GCATGCAGCA ATTACTGGAT
    TATCCAGAAG ATGATGTTGA GGAAACATTT TGTCTTAATT TTACGATCAC AGTTGAAAAC
    TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT CTAAATGGTG CAGACACAGC TGTTAACAAA
    CAAAATCGGC AGGAGTTTGT TGATGCTTAT GTGGATTACA TATTCAATAA ATCAGTGGCT
    TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT CATAAGGTCT GTGGAGGAAA GGTCCTTCAG
    CTCTTCCAGC CAAATGAACT ACAAGCAATG GTTATTGGAA ACACAAATTA TGATTGGAAG
    GAACTGGAAA AGAATACAGA ATACAAAGGG GAGTATTGGG CAGAGCATCC AACGATAAAA
    ATTTTTTGGG AAGTATTTCA TGAATTACCA TTGGAAAAGA AAAAACAATT TCTGTTATTT
    TTGACAGGTA GTGATCGAAT ACCTATTCTT GGTATGAAGA GTCTAAAACT AGTCATCCAG
    TCAACAGGAG GTGGTGAGGA ATATCTCCCA GTTTCCCATA CCTGTTTTAA TCTTCTGGAT
    CTTCCAAAAT ACACCGAAAA AGAAACTCTA CGCTCTAAGC TGATTCAAGC AATTGATCAT
    AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35985
    Clone ID Related Accession (Same CDS sequence) XM_014112841.1
    Accession Version XM_014112841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA
    AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT
    TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC
    ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC
    ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT
    GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT
    CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT
    CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT
    CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG
    GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA
    TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA
    AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT
    CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT
    CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT
    CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC
    TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC
    TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC
    ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA
    CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT
    AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA
    TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT
    CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA
    CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA
    CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT
    GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG
    TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC
    AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA
    GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT
    CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG
    AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT
    GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA
    GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA
    GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG
    TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT
    GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG
    GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT
    GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA
    GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT
    GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT
    CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG
    TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC
    CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG
    GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT
    TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA
    GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA
    GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA
    AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA
    GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968316.1
    CDS809..3886
    Translation

    Target ORF information:

    RefSeq Version XM_014112841.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGCAGCAC AAGTTGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA
    AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT
    TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC
    ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC
    ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT
    GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT
    CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT
    CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT
    CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG
    GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA
    TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA
    AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT
    CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT
    CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT
    CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC
    TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC
    TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC
    ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA
    CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT
    AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA
    TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT
    CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA
    CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA
    CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT
    GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG
    TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC
    AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA
    GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT
    CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG
    AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT
    GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA
    GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA
    GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG
    TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT
    GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG
    GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT
    GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA
    GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT
    GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT
    CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG
    TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC
    CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG
    GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT
    TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA
    GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA
    GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA
    AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA
    GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe35986
    Clone ID Related Accession (Same CDS sequence) XM_014112842.1
    Accession Version XM_014112842.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3078bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA
    AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT
    TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC
    ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC
    ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT
    GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT
    CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT
    CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT
    CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG
    GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA
    TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA
    AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT
    CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT
    CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT
    CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC
    TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC
    TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC
    ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA
    CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT
    AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA
    TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT
    CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA
    CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA
    CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT
    GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG
    TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC
    AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA
    GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT
    CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG
    AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT
    GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA
    GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA
    GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG
    TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT
    GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG
    GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT
    GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA
    GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT
    GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT
    CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG
    TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC
    CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG
    GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT
    TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA
    GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA
    GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA
    AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA
    GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968317.1
    CDS90..3167
    Translation

    Target ORF information:

    RefSeq Version XM_014112842.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112842.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAGTGGA TTGTAGAGCA GGTTGTTGCC CTGGATGCCC AAAATATCGT AGCTGTTTCA 
    TGTGGAGAAG CTCATACATT AGCTCTAAAT GACAAAGGCC AGGTGTATGC ATGGGGTCTC
    GATTCTGATG GACAGCTTGG CCTGCTAGGA TCAGAGGAAT GTATCAGAGT ACCCAGTAAT
    AGTTATAGGG ACAAAAGAGA ACTCTCCAGA GAAACAAGCT GCCTCCCGAA AATCATGGGT
    ATCGACACTC TGGTCAGAAC TTGCTCCGGA CTCTCATATG GCAGAATCAG AAATATTAAA
    AGTTTGTCAG ATATCCAGAT AGTACAGGTT GCTTGCGGTT ACTATCATTC ACTTGCACTT
    TCTAAAGCGA GTGAAGTCTT CTGTTGGGGA CAGAATAAAT ATGGCCAGTT GGGTTTAGGC
    ATTGACTGTA AAAAGCAAGC TTCACCACAG CTGATTAAGT CTTTGCTTGG AATTCCTTTC
    ATGCAAGTTG CAGCAGGAGG AGCCCATAGT TTTGTACTCA CCCTTTCTGG AGCTATTTTT
    GGATGGGGAC GCAACAAATT TGGTCAACTA GGTCTTAATG ATGAAAATGA TAGGTATGTT
    CCTAATTTAC TAAAGTCACT AAGAACTCAG AAAATAGTTT ATATTTGTTG TGGAGAAGAT
    CATACTGCAG CACTAACCAA GGAAGGTGGC GTCTTTACTT TTGGAGCTGG AGGGTATGGT
    CAGTTGGGTC ATAACTCTAC CAGTCATGAA ATAAACCCAC GGAAAGTTTT TGAACTTATG
    GGAAGCATTG TTACTCAGAT TGCTTGTGGA AGGCAGCACA CTTCTGCTTT TGTTCCTTCA
    TCAGGACGAA TTTATTCTTT TGGACTTGGT GGTAATGGGC AGCTAGGAAC TGGTTCAACA
    AGCAACAGGA AAAGTCCTTT TACTGTGAAA GGAAATTGGT TCCCTTATAA TGGGCAGTGT
    CCACCAGATA TTGATTCTGA AGAATATTTC TGTGTGAAAA GAATTTTCTC AGGTGGAGAT
    CAAAGCTTTT CACATTACTC TAATCCCCAG AACTGTGGGC CACCAGATGA CTTTAGATGT
    CCTGATCCTT CAAAGCAGAT CTGGACTGTG AATGAAACTC TAATTCAAAA ATGGCTGAGC
    TACCCATCTG GGAGGTTTCC TGTGGAGATA GCCAATGAAA TAGATGGAAC ATTTTCTTCC
    TCTGGCTGTC TAAATGGAAG TTTTCTAGCT GTTAGCAATG ATGATCACTA TAGAACAGGC
    ACCAAGTTTT CAGGGGTTGA TATGAATGCT GCTAGGCTTT TATTCCACAA ACTTATACAA
    CCTGATCATC CTCAGATATC TCAGCAGGTG GCAGCTAGTT TGGAAAAGAA TCTTATTCCT
    AAACTGACTA GTTCCTTACC TGATGTTGAA GCATTGAGGT TTTATCTTAC TCTACCAGAA
    TGTCCCCTGA TGAGTGATTC CAACAATTTC ACAACAATAG CAATTCCCTT TGGTACAGCT
    CTTGTAAATC TAGAAAAGGC ACCACTGAAA GTACTTGAAA ACTGGTGGTC AGTACTTGAA
    CCTCCACTAT TCCTCAAGAT TGTAGAACTT TTTAAGGAAG TTGTGGTACA TCTTTTGAAA
    CTCTACAAGA TCGGCATTCC CCCTTCTGAA AGAAGAATTT TCAACAGTTT TCTTCATACT
    GCATTAAAGG TTTTAGAAAT ATTGCATAAG GTAAATGAGA AAACGGGACA GATTATACAG
    TATGATAAAT TTTATATACA TGAAGTACAA GAATTGATAG ACATAAGGAA TGATTATATC
    AACTGGGTCC AACAGCAGGC CTATGGAATG GATGTCAACC ATGGATTAAC TGAGTTGGCA
    GATATCCCTG TTACAATCTG CACATATCCA TTTGTATTTG ATGCCCAAGC AAAAACTACT
    CTGTTACAAA CAGATGCAGT CTTACAGATG CAGATGGCTA TTGACCAGGC CCACAGACAG
    AATGTCTCCT CTCTTTTCCT CCCAGTGATT GAATCTGTGA ATCCCTGCTT AATTCTAGTT
    GTACGTAGAG AAAATATTGT AGGAGATGCA ATGGAAGTCC TTAGGAAAAC AAAGAATATA
    GACTACAAAA AGCCACTCAA GGTTATATTT GTTGGAGAAG ATGCTGTTGA TGCAGGAGGA
    GTACGCAAAG AATTTTTCTT GCTCATCATG AGAGAATTGT TGGATCCTAA ATATGGCATG
    TTTAGGTATT ATGAAGATTC CAGGCTCATT TGGTTTTCTG ATAAGACATT TGAAGACAGT
    GATTTGTTTC ATTTGATTGG TGTTATCTGT GGATTAGCAA TTTATAATTT TACTATTGTG
    GACCTCCACT TTCCTTTGGC TTTATATAAG AAACTGCTGA AAAAGAAGCC ATCCTTGGAT
    GATTTGAAGG AACTAGTGCC TGATGTTGGG AGAAGCATGC AGCAATTACT GGATTATCCA
    GAAGATGATG TTGAGGAAAC ATTTTGTCTT AATTTTACGA TCACAGTTGA AAACTTTGGT
    GCAACAGAAG TGAAAGAGCT GGTTCTAAAT GGTGCAGACA CAGCTGTTAA CAAACAAAAT
    CGGCAGGAGT TTGTTGATGC TTATGTGGAT TACATATTCA ATAAATCAGT GGCTTCCTTG
    TTTGATGCTT TTCATGCGGG CTTTCATAAG GTCTGTGGAG GAAAGGTCCT TCAGCTCTTC
    CAGCCAAATG AACTACAAGC AATGGTTATT GGAAACACAA ATTATGATTG GAAGGAACTG
    GAAAAGAATA CAGAATACAA AGGGGAGTAT TGGGCAGAGC ATCCAACGAT AAAAATTTTT
    TGGGAAGTAT TTCATGAATT ACCATTGGAA AAGAAAAAAC AATTTCTGTT ATTTTTGACA
    GGTAGTGATC GAATACCTAT TCTTGGTATG AAGAGTCTAA AACTAGTCAT CCAGTCAACA
    GGAGGTGGTG AGGAATATCT CCCAGTTTCC CATACCTGTT TTAATCTTCT GGATCTTCCA
    AAATACACCG AAAAAGAAAC TCTACGCTCT AAGCTGATTC AAGCAATTGA TCATAATGAA
    GGCTTCAGTT TAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04930
    Clone ID Related Accession (Same CDS sequence) XM_005618934.3 , XM_005618934.2
    Accession Version XM_005618934.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618934.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG
    GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618991.1
    CDS454..3699
    Translation

    Target ORF information:

    RefSeq Version XM_005618934.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618934.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG
    GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04930
    Clone ID Related Accession (Same CDS sequence) XM_005618934.3 , XM_005618934.2
    Accession Version XM_005618934.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618934.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG
    GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618991.1
    CDS465..3710
    Translation

    Target ORF information:

    RefSeq Version XM_005618934.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618934.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGCTGC CTCCCGAAAA TCATGGGTAT CGACACTCTG
    GTCAGAACTT GCTCCGGACT CTCATATGGC AGAATCAGAA ATATTAAAAG TTTGTCAGAT
    ATCCAGATAG TACAGGTTGC TTGCGGTTAC TATCATTCAC TTGCACTTTC TAAAGCGAGT
    GAAGTCTTCT GTTGGGGACA GAATAAATAT GGCCAGTTGG GTTTAGGCAT TGACTGTAAA
    AAGCAAGCTT CACCACAGCT GATTAAGTCT TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA
    GCAGGAGGAG CCCATAGTTT TGTACTCACC CTTTCTGGAG CTATTTTTGG ATGGGGACGC
    AACAAATTTG GTCAACTAGG TCTTAATGAT GAAAATGATA GGTATGTTCC TAATTTACTA
    AAGTCACTAA GAACTCAGAA AATAGTTTAT ATTTGTTGTG GAGAAGATCA TACTGCAGCA
    CTAACCAAGG AAGGTGGCGT CTTTACTTTT GGAGCTGGAG GGTATGGTCA GTTGGGTCAT
    AACTCTACCA GTCATGAAAT AAACCCACGG AAAGTTTTTG AACTTATGGG AAGCATTGTT
    ACTCAGATTG CTTGTGGAAG GCAGCACACT TCTGCTTTTG TTCCTTCATC AGGACGAATT
    TATTCTTTTG GACTTGGTGG TAATGGGCAG CTAGGAACTG GTTCAACAAG CAACAGGAAA
    AGTCCTTTTA CTGTGAAAGG AAATTGGTTC CCTTATAATG GGCAGTGTCC ACCAGATATT
    GATTCTGAAG AATATTTCTG TGTGAAAAGA ATTTTCTCAG GTGGAGATCA AAGCTTTTCA
    CATTACTCTA ATCCCCAGAA CTGTGGGCCA CCAGATGACT TTAGATGTCC TGATCCTTCA
    AAGCAGATCT GGACTGTGAA TGAAACTCTA ATTCAAAAAT GGCTGAGCTA CCCATCTGGG
    AGGTTTCCTG TGGAGATAGC CAATGAAATA GATGGAACAT TTTCTTCCTC TGGCTGTCTA
    AATGGAAGTT TTCTAGCTGT TAGCAATGAT GATCACTATA GAACAGGCAC CAAGTTTTCA
    GGGGTTGATA TGAATGCTGC TAGGCTTTTA TTCCACAAAC TTATACAACC TGATCATCCT
    CAGATATCTC AGCAGGTGGC AGCTAGTTTG GAAAAGAATC TTATTCCTAA ACTGACTAGT
    TCCTTACCTG ATGTTGAAGC ATTGAGGTTT TATCTTACTC TACCAGAATG TCCCCTGATG
    AGTGATTCCA ACAATTTCAC AACAATAGCA ATTCCCTTTG GTACAGCTCT TGTAAATCTA
    GAAAAGGCAC CACTGAAAGT ACTTGAAAAC TGGTGGTCAG TACTTGAACC TCCACTATTC
    CTCAAGATTG TAGAACTTTT TAAGGAAGTT GTGGTACATC TTTTGAAACT CTACAAGATC
    GGCATTCCCC CTTCTGAAAG AAGAATTTTC AACAGTTTTC TTCATACTGC ATTAAAGGTT
    TTAGAAATAT TGCATAAGGT AAATGAGAAA ACGGGACAGA TTATACAGTA TGATAAATTT
    TATATACATG AAGTACAAGA ATTGATAGAC ATAAGGAATG ATTATATCAA CTGGGTCCAA
    CAGCAGGCCT ATGGAATGGA TGTCAACCAT GGATTAACTG AGTTGGCAGA TATCCCTGTT
    ACAATCTGCA CATATCCATT TGTATTTGAT GCCCAAGCAA AAACTACTCT GTTACAAACA
    GATGCAGTCT TACAGATGCA GATGGCTATT GACCAGGCCC ACAGACAGAA TGTCTCCTCT
    CTTTTCCTCC CAGTGATTGA ATCTGTGAAT CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA
    AATATTGTAG GAGATGCAAT GGAAGTCCTT AGGAAAACAA AGAATATAGA CTACAAAAAG
    CCACTCAAGG TTATATTTGT TGGAGAAGAT GCTGTTGATG CAGGAGGAGT ACGCAAAGAA
    TTTTTCTTGC TCATCATGAG AGAATTGTTG GATCCTAAAT ATGGCATGTT TAGGTATTAT
    GAAGATTCCA GGCTCATTTG GTTTTCTGAT AAGACATTTG AAGACAGTGA TTTGTTTCAT
    TTGATTGGTG TTATCTGTGG ATTAGCAATT TATAATTTTA CTATTGTGGA CCTCCACTTT
    CCTTTGGCTT TATATAAGAA ACTGCTGAAA AAGAAGCCAT CCTTGGATGA TTTGAAGGAA
    CTAGTGCCTG ATGTTGGGAG AAGCATGCAG CAATTACTGG ATTATCCAGA AGATGATGTT
    GAGGAAACAT TTTGTCTTAA TTTTACGATC ACAGTTGAAA ACTTTGGTGC AACAGAAGTG
    AAAGAGCTGG TTCTAAATGG TGCAGACACA GCTGTTAACA AACAAAATCG GCAGGAGTTT
    GTTGATGCTT ATGTGGATTA CATATTCAAT AAATCAGTGG CTTCCTTGTT TGATGCTTTT
    CATGCGGGCT TTCATAAGGT CTGTGGAGGA AAGGTCCTTC AGCTCTTCCA GCCAAATGAA
    CTACAAGCAA TGGTTATTGG AAACACAAAT TATGATTGGA AGGAACTGGA AAAGAATACA
    GAATACAAAG GGGAGTATTG GGCAGAGCAT CCAACGATAA AAATTTTTTG GGAAGTATTT
    CATGAATTAC CATTGGAAAA GAAAAAACAA TTTCTGTTAT TTTTGACAGG TAGTGATCGA
    ATACCTATTC TTGGTATGAA GAGTCTAAAA CTAGTCATCC AGTCAACAGG AGGTGGTGAG
    GAATATCTCC CAGTTTCCCA TACCTGTTTT AATCTTCTGG ATCTTCCAAA ATACACCGAA
    AAAGAAACTC TACGCTCTAA GCTGATTCAA GCAATTGATC ATAATGAAGG CTTCAGTTTA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04932
    Clone ID Related Accession (Same CDS sequence) XM_005618937.3 , XM_005618937.2
    Accession Version XM_005618937.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005618937.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618994.1
    CDS454..3669
    Translation

    Target ORF information:

    RefSeq Version XM_005618937.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618937.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04932
    Clone ID Related Accession (Same CDS sequence) XM_005618937.3 , XM_005618937.2
    Accession Version XM_005618937.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618994.1
    CDS464..3679
    Translation

    Target ORF information:

    RefSeq Version XM_005618937.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGTAAT AGTTATAGGG ACAAAAGAGA ACTCTCCAGA
    GAAACAAGAA ATATTAAAAG TTTGTCAGAT ATCCAGATAG TACAGGTTGC TTGCGGTTAC
    TATCATTCAC TTGCACTTTC TAAAGCGAGT GAAGTCTTCT GTTGGGGACA GAATAAATAT
    GGCCAGTTGG GTTTAGGCAT TGACTGTAAA AAGCAAGCTT CACCACAGCT GATTAAGTCT
    TTGCTTGGAA TTCCTTTCAT GCAAGTTGCA GCAGGAGGAG CCCATAGTTT TGTACTCACC
    CTTTCTGGAG CTATTTTTGG ATGGGGACGC AACAAATTTG GTCAACTAGG TCTTAATGAT
    GAAAATGATA GGTATGTTCC TAATTTACTA AAGTCACTAA GAACTCAGAA AATAGTTTAT
    ATTTGTTGTG GAGAAGATCA TACTGCAGCA CTAACCAAGG AAGGTGGCGT CTTTACTTTT
    GGAGCTGGAG GGTATGGTCA GTTGGGTCAT AACTCTACCA GTCATGAAAT AAACCCACGG
    AAAGTTTTTG AACTTATGGG AAGCATTGTT ACTCAGATTG CTTGTGGAAG GCAGCACACT
    TCTGCTTTTG TTCCTTCATC AGGACGAATT TATTCTTTTG GACTTGGTGG TAATGGGCAG
    CTAGGAACTG GTTCAACAAG CAACAGGAAA AGTCCTTTTA CTGTGAAAGG AAATTGGTTC
    CCTTATAATG GGCAGTGTCC ACCAGATATT GATTCTGAAG AATATTTCTG TGTGAAAAGA
    ATTTTCTCAG GTGGAGATCA AAGCTTTTCA CATTACTCTA ATCCCCAGAA CTGTGGGCCA
    CCAGATGACT TTAGATGTCC TGATCCTTCA AAGCAGATCT GGACTGTGAA TGAAACTCTA
    ATTCAAAAAT GGCTGAGCTA CCCATCTGGG AGGTTTCCTG TGGAGATAGC CAATGAAATA
    GATGGAACAT TTTCTTCCTC TGGCTGTCTA AATGGAAGTT TTCTAGCTGT TAGCAATGAT
    GATCACTATA GAACAGGCAC CAAGTTTTCA GGGGTTGATA TGAATGCTGC TAGGCTTTTA
    TTCCACAAAC TTATACAACC TGATCATCCT CAGATATCTC AGCAGGTGGC AGCTAGTTTG
    GAAAAGAATC TTATTCCTAA ACTGACTAGT TCCTTACCTG ATGTTGAAGC ATTGAGGTTT
    TATCTTACTC TACCAGAATG TCCCCTGATG AGTGATTCCA ACAATTTCAC AACAATAGCA
    ATTCCCTTTG GTACAGCTCT TGTAAATCTA GAAAAGGCAC CACTGAAAGT ACTTGAAAAC
    TGGTGGTCAG TACTTGAACC TCCACTATTC CTCAAGATTG TAGAACTTTT TAAGGAAGTT
    GTGGTACATC TTTTGAAACT CTACAAGATC GGCATTCCCC CTTCTGAAAG AAGAATTTTC
    AACAGTTTTC TTCATACTGC ATTAAAGGTT TTAGAAATAT TGCATAAGGT AAATGAGAAA
    ACGGGACAGA TTATACAGTA TGATAAATTT TATATACATG AAGTACAAGA ATTGATAGAC
    ATAAGGAATG ATTATATCAA CTGGGTCCAA CAGCAGGCCT ATGGAATGGA TGTCAACCAT
    GGATTAACTG AGTTGGCAGA TATCCCTGTT ACAATCTGCA CATATCCATT TGTATTTGAT
    GCCCAAGCAA AAACTACTCT GTTACAAACA GATGCAGTCT TACAGATGCA GATGGCTATT
    GACCAGGCCC ACAGACAGAA TGTCTCCTCT CTTTTCCTCC CAGTGATTGA ATCTGTGAAT
    CCCTGCTTAA TTCTAGTTGT ACGTAGAGAA AATATTGTAG GAGATGCAAT GGAAGTCCTT
    AGGAAAACAA AGAATATAGA CTACAAAAAG CCACTCAAGG TTATATTTGT TGGAGAAGAT
    GCTGTTGATG CAGGAGGAGT ACGCAAAGAA TTTTTCTTGC TCATCATGAG AGAATTGTTG
    GATCCTAAAT ATGGCATGTT TAGGTATTAT GAAGATTCCA GGCTCATTTG GTTTTCTGAT
    AAGACATTTG AAGACAGTGA TTTGTTTCAT TTGATTGGTG TTATCTGTGG ATTAGCAATT
    TATAATTTTA CTATTGTGGA CCTCCACTTT CCTTTGGCTT TATATAAGAA ACTGCTGAAA
    AAGAAGCCAT CCTTGGATGA TTTGAAGGAA CTAGTGCCTG ATGTTGGGAG AAGCATGCAG
    CAATTACTGG ATTATCCAGA AGATGATGTT GAGGAAACAT TTTGTCTTAA TTTTACGATC
    ACAGTTGAAA ACTTTGGTGC AACAGAAGTG AAAGAGCTGG TTCTAAATGG TGCAGACACA
    GCTGTTAACA AACAAAATCG GCAGGAGTTT GTTGATGCTT ATGTGGATTA CATATTCAAT
    AAATCAGTGG CTTCCTTGTT TGATGCTTTT CATGCGGGCT TTCATAAGGT CTGTGGAGGA
    AAGGTCCTTC AGCTCTTCCA GCCAAATGAA CTACAAGCAA TGGTTATTGG AAACACAAAT
    TATGATTGGA AGGAACTGGA AAAGAATACA GAATACAAAG GGGAGTATTG GGCAGAGCAT
    CCAACGATAA AAATTTTTTG GGAAGTATTT CATGAATTAC CATTGGAAAA GAAAAAACAA
    TTTCTGTTAT TTTTGACAGG TAGTGATCGA ATACCTATTC TTGGTATGAA GAGTCTAAAA
    CTAGTCATCC AGTCAACAGG AGGTGGTGAG GAATATCTCC CAGTTTCCCA TACCTGTTTT
    AATCTTCTGG ATCTTCCAAA ATACACCGAA AAAGAAACTC TACGCTCTAA GCTGATTCAA
    GCAATTGATC ATAATGAAGG CTTCAGTTTA ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04933
    Clone ID Related Accession (Same CDS sequence) XM_844715.5 , XM_844715.4
    Accession Version XM_844715.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_844715.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA
    TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA
    GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC
    ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT
    GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG
    GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT
    GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA
    CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_849808.1
    CDS454..3627
    Translation

    Target ORF information:

    RefSeq Version XM_844715.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_844715.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA
    TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA
    GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC
    ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT
    GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG
    GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT
    GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA
    CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04933
    Clone ID Related Accession (Same CDS sequence) XM_844715.5 , XM_844715.4
    Accession Version XM_844715.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3174bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_844715.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA
    TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA
    GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC
    ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT
    GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG
    GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT
    GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA
    CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_849808.1
    CDS464..3637
    Translation

    Target ORF information:

    RefSeq Version XM_844715.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_844715.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGGATG TCAACCATGG ATTAACTGAG TTGGCAGATA TCCCTGTTAC AATCTGCACA
    TATCCATTTG TATTTGATGC CCAAGCAAAA ACTACTCTGT TACAAACAGA TGCAGTCTTA
    CAGATGCAGA TGGCTATTGA CCAGGCCCAC AGACAGAATG TCTCCTCTCT TTTCCTCCCA
    GTGATTGAAT CTGTGAATCC CTGCTTAATT CTAGTTGTAC GTAGAGAAAA TATTGTAGGA
    GATGCAATGG AAGTCCTTAG GAAAACAAAG AATATAGACT ACAAAAAGCC ACTCAAGGTT
    ATATTTGTTG GAGAAGATGC TGTTGATGCA GGAGGAGTAC GCAAAGAATT TTTCTTGCTC
    ATCATGAGAG AATTGTTGGA TCCTAAATAT GGCATGTTTA GGTATTATGA AGATTCCAGG
    CTCATTTGGT TTTCTGATAA GACATTTGAA GACAGTGATT TGTTTCATTT GATTGGTGTT
    ATCTGTGGAT TAGCAATTTA TAATTTTACT ATTGTGGACC TCCACTTTCC TTTGGCTTTA
    TATAAGAAAC TGCTGAAAAA GAAGCCATCC TTGGATGATT TGAAGGAACT AGTGCCTGAT
    GTTGGGAGAA GCATGCAGCA ATTACTGGAT TATCCAGAAG ATGATGTTGA GGAAACATTT
    TGTCTTAATT TTACGATCAC AGTTGAAAAC TTTGGTGCAA CAGAAGTGAA AGAGCTGGTT
    CTAAATGGTG CAGACACAGC TGTTAACAAA CAAAATCGGC AGGAGTTTGT TGATGCTTAT
    GTGGATTACA TATTCAATAA ATCAGTGGCT TCCTTGTTTG ATGCTTTTCA TGCGGGCTTT
    CATAAGGTCT GTGGAGGAAA GGTCCTTCAG CTCTTCCAGC CAAATGAACT ACAAGCAATG
    GTTATTGGAA ACACAAATTA TGATTGGAAG GAACTGGAAA AGAATACAGA ATACAAAGGG
    GAGTATTGGG CAGAGCATCC AACGATAAAA ATTTTTTGGG AAGTATTTCA TGAATTACCA
    TTGGAAAAGA AAAAACAATT TCTGTTATTT TTGACAGGTA GTGATCGAAT ACCTATTCTT
    GGTATGAAGA GTCTAAAACT AGTCATCCAG TCAACAGGAG GTGGTGAGGA ATATCTCCCA
    GTTTCCCATA CCTGTTTTAA TCTTCTGGAT CTTCCAAAAT ACACCGAAAA AGAAACTCTA
    CGCTCTAAGC TGATTCAAGC AATTGATCAT AATGAAGGCT TCAGTTTAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04934
    Clone ID Related Accession (Same CDS sequence) XM_536367.5 , XM_536367.6
    Accession Version XM_536367.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726056.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536367.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC
    TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT
    AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG
    CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA
    CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC
    CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG
    ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG
    TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT
    GATCATAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536367.2
    CDS463..3612
    Translation

    Target ORF information:

    RefSeq Version XM_536367.5
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536367.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC
    TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT
    AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG
    CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA
    CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC
    CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG
    ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG
    TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT
    GATCATAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04934
    Clone ID Related Accession (Same CDS sequence) XM_536367.5 , XM_536367.6
    Accession Version XM_536367.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3150bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product probable E3 ubiquitin-protein ligase HERC4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006586.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536367.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC
    TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT
    AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG
    CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA
    CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC
    CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG
    ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG
    TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT
    GATCATAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536367.2
    CDS453..3602
    Translation

    Target ORF information:

    RefSeq Version XM_536367.6
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT and RLD domain containing E3 ubiquitin protein ligase 4 (HERC4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536367.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTGTGCT GGGGAAATGC ATCTTTTGGA CAGCTAGGTT TGGGTGGAAT TGATGAAGAA 
    ATTGTACTAG AGCCCAGAAA AAGTGACTTT TTTATAAATA AAAAGGTCCG AGATGTGGGA
    TGTGGACTCA GACATACTGT ATTTGTCCTG GATGATGGAA CAGTGTACAC ATGTGGATGT
    AATGATCTAG GACAGCTGGG TCATGAAAAA TCCAGAAAGA AACCAGAGCA GGTTGTTGCC
    CTGGATGCCC AAAATATCGT AGCTGTTTCA TGTGGAGAAG CTCATACATT AGCTCTAAAT
    GACAAAGGCC AGGTGTATGC ATGGGGTCTC GATTCTGATG GACAGCTTGG CCTGCTAGGA
    TCAGAGGAAT GTATCAGAGT ACCCAGAAAT ATTAAAAGTT TGTCAGATAT CCAGATAGTA
    CAGGTTGCTT GCGGTTACTA TCATTCACTT GCACTTTCTA AAGCGAGTGA AGTCTTCTGT
    TGGGGACAGA ATAAATATGG CCAGTTGGGT TTAGGCATTG ACTGTAAAAA GCAAGCTTCA
    CCACAGCTGA TTAAGTCTTT GCTTGGAATT CCTTTCATGC AAGTTGCAGC AGGAGGAGCC
    CATAGTTTTG TACTCACCCT TTCTGGAGCT ATTTTTGGAT GGGGACGCAA CAAATTTGGT
    CAACTAGGTC TTAATGATGA AAATGATAGG TATGTTCCTA ATTTACTAAA GTCACTAAGA
    ACTCAGAAAA TAGTTTATAT TTGTTGTGGA GAAGATCATA CTGCAGCACT AACCAAGGAA
    GGTGGCGTCT TTACTTTTGG AGCTGGAGGG TATGGTCAGT TGGGTCATAA CTCTACCAGT
    CATGAAATAA ACCCACGGAA AGTTTTTGAA CTTATGGGAA GCATTGTTAC TCAGATTGCT
    TGTGGAAGGC AGCACACTTC TGCTTTTGTT CCTTCATCAG GACGAATTTA TTCTTTTGGA
    CTTGGTGGTA ATGGGCAGCT AGGAACTGGT TCAACAAGCA ACAGGAAAAG TCCTTTTACT
    GTGAAAGGAA ATTGGTTCCC TTATAATGGG CAGTGTCCAC CAGATATTGA TTCTGAAGAA
    TATTTCTGTG TGAAAAGAAT TTTCTCAGGT GGAGATCAAA GCTTTTCACA TTACTCTAAT
    CCCCAGAACT GTGGGCCACC AGATGACTTT AGATGTCCTG ATCCTTCAAA GCAGATCTGG
    ACTGTGAATG AAACTCTAAT TCAAAAATGG CTGAGCTACC CATCTGGGAG GTTTCCTGTG
    GAGATAGCCA ATGAAATAGA TGGAACATTT TCTTCCTCTG GCTGTCTAAA TGGAAGTTTT
    CTAGCTGTTA GCAATGATGA TCACTATAGA ACAGGCACCA AGTTTTCAGG GGTTGATATG
    AATGCTGCTA GGCTTTTATT CCACAAACTT ATACAACCTG ATCATCCTCA GATATCTCAG
    CAGGTGGCAG CTAGTTTGGA AAAGAATCTT ATTCCTAAAC TGACTAGTTC CTTACCTGAT
    GTTGAAGCAT TGAGGTTTTA TCTTACTCTA CCAGAATGTC CCCTGATGAG TGATTCCAAC
    AATTTCACAA CAATAGCAAT TCCCTTTGGT ACAGCTCTTG TAAATCTAGA AAAGGCACCA
    CTGAAAGTAC TTGAAAACTG GTGGTCAGTA CTTGAACCTC CACTATTCCT CAAGATTGTA
    GAACTTTTTA AGGAAGTTGT GGTACATCTT TTGAAACTCT ACAAGATCGG CATTCCCCCT
    TCTGAAAGAA GAATTTTCAA CAGTTTTCTT CATACTGCAT TAAAGGTTTT AGAAATATTG
    CATAAGGTAA ATGAGAAAAC GGGACAGATT ATACAGTATG ATAAATTTTA TATACATGAA
    GTACAAGAAT TGATAGACAT AAGGAATGAT TATATCAACT GGGTCCAACA GCAGGCCTAT
    GGAATGTTGG CAGATATCCC TGTTACAATC TGCACATATC CATTTGTATT TGATGCCCAA
    GCAAAAACTA CTCTGTTACA AACAGATGCA GTCTTACAGA TGCAGATGGC TATTGACCAG
    GCCCACAGAC AGAATGTCTC CTCTCTTTTC CTCCCAGTGA TTGAATCTGT GAATCCCTGC
    TTAATTCTAG TTGTACGTAG AGAAAATATT GTAGGAGATG CAATGGAAGT CCTTAGGAAA
    ACAAAGAATA TAGACTACAA AAAGCCACTC AAGGTTATAT TTGTTGGAGA AGATGCTGTT
    GATGCAGGAG GAGTACGCAA AGAATTTTTC TTGCTCATCA TGAGAGAATT GTTGGATCCT
    AAATATGGCA TGTTTAGGTA TTATGAAGAT TCCAGGCTCA TTTGGTTTTC TGATAAGACA
    TTTGAAGACA GTGATTTGTT TCATTTGATT GGTGTTATCT GTGGATTAGC AATTTATAAT
    TTTACTATTG TGGACCTCCA CTTTCCTTTG GCTTTATATA AGAAACTGCT GAAAAAGAAG
    CCATCCTTGG ATGATTTGAA GGAACTAGTG CCTGATGTTG GGAGAAGCAT GCAGCAATTA
    CTGGATTATC CAGAAGATGA TGTTGAGGAA ACATTTTGTC TTAATTTTAC GATCACAGTT
    GAAAACTTTG GTGCAACAGA AGTGAAAGAG CTGGTTCTAA ATGGTGCAGA CACAGCTGTT
    AACAAACAAA ATCGGCAGGA GTTTGTTGAT GCTTATGTGG ATTACATATT CAATAAATCA
    GTGGCTTCCT TGTTTGATGC TTTTCATGCG GGCTTTCATA AGGTCTGTGG AGGAAAGGTC
    CTTCAGCTCT TCCAGCCAAA TGAACTACAA GCAATGGTTA TTGGAAACAC AAATTATGAT
    TGGAAGGAAC TGGAAAAGAA TACAGAATAC AAAGGGGAGT ATTGGGCAGA GCATCCAACG
    ATAAAAATTT TTTGGGAAGT ATTTCATGAA TTACCATTGG AAAAGAAAAA ACAATTTCTG
    TTATTTTTGA CAGGTAGTGA TCGAATACCT ATTCTTGGTA TGAAGAGTCT AAAACTAGTC
    ATCCAGTCAA CAGGAGGTGG TGAGGAATAT CTCCCAGTTT CCCATACCTG TTTTAATCTT
    CTGGATCTTC CAAAATACAC CGAAAAAGAA ACTCTACGCT CTAAGCTGAT TCAAGCAATT
    GATCATAATG AAGGCTTCAG TTTAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.