N4BP2 cDNA ORF clone, Canis lupus familiaris(dog)

The following N4BP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the N4BP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287389 XM_022417214.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287389 XM_022417217.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287389 XM_022417211.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287389 XM_022417215.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287389 XM_022417212.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287389 XM_022417216.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287390 XM_022417218.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287391 XM_022417223.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287391 XM_022417222.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287391 XM_022417221.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287391 XM_022417220.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OCe287392 XM_022417225.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe04566 XM_005618608.2
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04566 XM_005618607.2
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04566 XM_014112633.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04566 XM_022417219.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04566 XM_005618606.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04566 XM_005618605.2
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04566 XM_536248.4
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04567 XM_014112634.1
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04567 XM_005618610.2
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OCe04567 XM_005618609.2
Latest version!
Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287389
    Clone ID Related Accession (Same CDS sequence) XM_022417211.1 , XM_022417212.1 , XM_022417214.1 , XM_022417217.1 , XM_022417215.1 , XM_022417216.1
    Accession Version XM_022417211.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272919.1
    CDS725..6139
    Translation

    Target ORF information:

    RefSeq Version XM_022417211.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417211.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287389
    Clone ID Related Accession (Same CDS sequence) XM_022417211.1 , XM_022417212.1 , XM_022417214.1 , XM_022417217.1 , XM_022417215.1 , XM_022417216.1
    Accession Version XM_022417212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272920.1
    CDS536..5950
    Translation

    Target ORF information:

    RefSeq Version XM_022417212.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287389
    Clone ID Related Accession (Same CDS sequence) XM_022417211.1 , XM_022417212.1 , XM_022417214.1 , XM_022417217.1 , XM_022417215.1 , XM_022417216.1
    Accession Version XM_022417214.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272922.1
    CDS619..6033
    Translation

    Target ORF information:

    RefSeq Version XM_022417214.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417214.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287389
    Clone ID Related Accession (Same CDS sequence) XM_022417211.1 , XM_022417212.1 , XM_022417214.1 , XM_022417217.1 , XM_022417215.1 , XM_022417216.1
    Accession Version XM_022417217.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272925.1
    CDS251..5665
    Translation

    Target ORF information:

    RefSeq Version XM_022417217.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417217.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287389
    Clone ID Related Accession (Same CDS sequence) XM_022417211.1 , XM_022417212.1 , XM_022417214.1 , XM_022417217.1 , XM_022417215.1 , XM_022417216.1
    Accession Version XM_022417215.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272923.1
    CDS642..6056
    Translation

    Target ORF information:

    RefSeq Version XM_022417215.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417215.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287389
    Clone ID Related Accession (Same CDS sequence) XM_022417211.1 , XM_022417212.1 , XM_022417214.1 , XM_022417217.1 , XM_022417215.1 , XM_022417216.1
    Accession Version XM_022417216.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272924.1
    CDS440..5854
    Translation

    Target ORF information:

    RefSeq Version XM_022417216.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417216.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTCTCTGAAA TTAAACCAGG GTGCTTGAAA
    GTCATGCTAA AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287390
    Clone ID Related Accession (Same CDS sequence) XM_022417218.1
    Accession Version XM_022417218.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5394bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTGCCCCCGG GGTGCAGGCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272926.1
    CDS728..6121
    Translation

    Target ORF information:

    RefSeq Version XM_022417218.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGA AAAGGGAGGC ACTTATGTTT GAAAAAGATT GTGCCACTAA ACTAAAGGAG
    AAGCAGCTCT TTAAGATATT TCCAGCCATT AACCAAAATT TTCTGGTGGA CATTTTCAAG
    GACCACAACT ATTCATTAGA ACACACAGTT CAGTTTCTAA ACTGTGTTCT TGAAGGAGAC
    CCTGTAAAAA CGGTCGTAGC GCAAGAGTTT GTTCACCCAA ATGAGAATGT CACTTCTCAT
    ACTACACAGA AGTCTAAGGA GAAAAAGGCA AAGAAATCGA AAGAGACTGA AGATATACCA
    AGTGAACCAT CTTTCCAGGA TTTTGAGTAC CCAGAGTACA ATGACTACAG GGCAGAGGCT
    TTTCTGCACC AACAGAAGAG GATGGAATGC TACAGCAAAG CGAAAGAAGC TTATCGGATG
    GGGAAAAAAC ACGTTGCCAC GTTTTATGCC CAGCAGGGTA GTCTCCATGA GCAGAAGATG
    AAAGAAGCCA ATCACCTTGC TGCTGTGGAG ATCTTTGAGA AAGTCAATGC CTCCCTGCTG
    CCACAGAATG TTTTAGACCT CCATGGGCTG CATGTGGATG AAGCTATAGA ACATTTGATG
    ACAGTTTTAC AGCAGAAGAC TGAAGAATTT AAGCAGAATG GTGGTAAGTC CTATCTCTCT
    GTGATTACGG GGAGAGGAAA CCACAGCCAA GGAGGAGTTG CACGCATCAA ACCAGCCGTC
    ATTAAATACC TCATAAGCCA TAGCTTCAGG TTGCCCCCGG GGTGCAGGCT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287391
    Clone ID Related Accession (Same CDS sequence) XM_022417220.1 , XM_022417221.1 , XM_022417222.1 , XM_022417223.1
    Accession Version XM_022417220.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272928.1
    CDS242..5419
    Translation

    Target ORF information:

    RefSeq Version XM_022417220.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417220.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287391
    Clone ID Related Accession (Same CDS sequence) XM_022417220.1 , XM_022417221.1 , XM_022417222.1 , XM_022417223.1
    Accession Version XM_022417221.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272929.1
    CDS518..5695
    Translation

    Target ORF information:

    RefSeq Version XM_022417221.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417221.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287391
    Clone ID Related Accession (Same CDS sequence) XM_022417220.1 , XM_022417221.1 , XM_022417222.1 , XM_022417223.1
    Accession Version XM_022417222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272930.1
    CDS425..5602
    Translation

    Target ORF information:

    RefSeq Version XM_022417222.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287391
    Clone ID Related Accession (Same CDS sequence) XM_022417220.1 , XM_022417221.1 , XM_022417222.1 , XM_022417223.1
    Accession Version XM_022417223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272931.1
    CDS531..5708
    Translation

    Target ORF information:

    RefSeq Version XM_022417223.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGTTTC TGCCCATTGA GGAGAATATG
    TTTTTGAGGA CGTTGTTCAC AGCATCACAC AGTGTCCATT TTATGAGGAC CCATGGGAGA
    AATTTCTCTC AGAAAAGGGA GGCACTTATG TTTGAAAAAG ATTGTGCCAC TAAACTAAAG
    GAGAAGCAGC TCTTTAAGAT ATTTCCAGCC ATTAACCAAA ATTTTCTGGT GGACATTTTC
    AAGGACCACA ACTATTCATT AGAACACACA GTTCAGTTTC TAAACTGTGT TCTTGAAGGA
    GACCCTGTAA AAACGGTCGT AGCGCAAGAG TTTGTTCACC CAAATGAGAA TGTCACTTCT
    CATACTACAC AGAAGTCTAA GGAGAAAAAG GCAAAGAAAT CGAAAGAGAC TGAAGATATA
    CCAAGTGAAC CATCTTTCCA GGATTTTGAG TACCCAGAGT ACAATGACTA CAGGGCAGAG
    GCTTTTCTGC ACCAACAGAA GAGGATGGAA TGCTACAGCA AAGCGAAAGA AGCTTATCGG
    ATGGGGAAAA AACACGTTGC CACGTTTTAT GCCCAGCAGG GTAGTCTCCA TGAGCAGAAG
    ATGAAAGAAG CCAATCACCT TGCTGCTGTG GAGATCTTTG AGAAAGTCAA TGCCTCCCTG
    CTGCCACAGA ATGTTTTAGA CCTCCATGGG CTGCATGTGG ATGAAGCTAT AGAACATTTG
    ATGACAGTTT TACAGCAGAA GACTGAAGAA TTTAAGCAGA ATGGTGGTAA GTCCTATCTC
    TCTGTGATTA CGGGGAGAGG AAACCACAGC CAAGGAGGAG TTGCACGCAT CAAACCAGCC
    GTCATTAAAT ACCTCATAAG CCATAGCTTC AGGTTCTCTG AAATTAAACC AGGGTGCTTG
    AAAGTCATGC TAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe287392
    Clone ID Related Accession (Same CDS sequence) XM_022417225.1
    Accession Version XM_022417225.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4671bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGG TCTCAGTACC AGAAAAAGCT GTACTTCTCC TGAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272933.1
    CDS746..5416
    Translation

    Target ORF information:

    RefSeq Version XM_022417225.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417225.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGTTTCTGC CCATTGAGGA GAATATGTTT
    TTGAGGACGT TGTTCACAGC ATCACACAGT GTCCATTTTA TGAGGACCCA TGGGAGAAAT
    TTCTCTCAGG TCTCAGTACC AGAAAAAGCT GTACTTCTCC TGAGCAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_005618605.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618662.1
    CDS872..6190
    Translation

    Target ORF information:

    RefSeq Version XM_005618605.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618605.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_005618607.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618664.1
    CDS789..6107
    Translation

    Target ORF information:

    RefSeq Version XM_005618607.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618607.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_005618606.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618663.1
    CDS433..5751
    Translation

    Target ORF information:

    RefSeq Version XM_005618606.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618606.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_005618608.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618608.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618665.1
    CDS766..6084
    Translation

    Target ORF information:

    RefSeq Version XM_005618608.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618608.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_536248.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536248.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_536248.2
    CDS683..6001
    Translation

    Target ORF information:

    RefSeq Version XM_536248.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_536248.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_014112633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968108.1
    CDS429..5747
    Translation

    Target ORF information:

    RefSeq Version XM_014112633.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04566
    Clone ID Related Accession (Same CDS sequence) XM_005618605.2 , XM_005618607.2 , XM_005618606.1 , XM_005618608.2 , XM_536248.4 , XM_014112633.1 , XM_022417219.1
    Accession Version XM_022417219.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product NEDD4-binding protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006585.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022272927.1
    CDS198..5516
    Translation

    Target ORF information:

    RefSeq Version XM_022417219.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022417219.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCCAAGGA GAAAGAAAAA TCTTGGGGGA AATCCTTTTC GGAAGACTGC AAGCTCTCAG 
    GAAGTTGTAT CCAGTGTGGC TAGTCGTGAG GAGCCACCCA CTACTCTTCC TTCCATGTGT
    GAGACAAAAG TTGATCGGGA GGAACTCTTC ACCAGTATCT CAGAGATGTT TTCTGATCTG
    GACCCCGATG TAGTGTATTT GATGCTTTCT GAATGTGATT TCAAAGTTGA AAATGCAATG
    GATTGTCTAT TAGAATTATC TGCCACTGAT GCCAAGGTAG AAGAATCATC TTCAAAAAGC
    TTTGTTGCCT CCGAGAACCA AGTAAGTGCT GTGGGACGTG AAATAATGGA AAAATATACT
    CCTGAAGAAG AGAGGGAAGA TTCAAAAATG GATTCATTTT TGGACATGCA GCTAACTGAA
    GACTTGGATT CCTTAATACA GAATGCTTTT GAGAAATTGA ACTCTTCTCC TGATGACCAA
    GTATACTCAT TTTTGCCTTT GCAAGGTGTT AATAGTTTTA GTGACCCGAG TGCATTTATA
    AATTCAGATT CAAGTAATAT GACTCCCATT CTTTCTACAC AGAATATGGA TTTGGACAGT
    GAAAATTTAG AGAATTCTGC CTCTGCGTTA AGTTCAAACA CCTTAATTTC ACATTCTGTT
    TCTGATGAGT CCAAAAATTT TATAAAGGAT GACGTATTGG CATTGGAAGA TCCTCTTCTC
    AGTAGTTCTT TAAATGTAGC AAATAACACT ATGGTGAGTA GTAGCAATCT CAGTCAAAGG
    CAGAAAGAGC TTTTAGAATC TGAGTGTGTT GAGACTCCGT TCTCTCAAGC CCCTGTAGAT
    TTGGATGCCA GTGAACCTCA GGCTCCTCTA AGCCTTACTG CGCAAAATTT GGGGCTTGAT
    TTACTAGGTA CAAGTGGGGA TCAGAAATCT GCCTCTGTCC CCGATGCATT TGTACCTTCT
    GAAGAATTCA GTTTCAAACC ACACAAACAT ACTGAACTTC CGTCAAAGGG GAAAGATGTG
    AATTACTGTC CTGTACTTAC CCCTCTCCCT CTGCTGCTGC CTCCTCCTCC ACCTCCACCA
    ATGTGGAATC CAATGATTCC TGCTTTTGAC CTCTTTCAAG GAAATCATGG CTTTATAGCT
    CCTGTTGTAA CCACCGCTAC GCACTGGAGA CCGGTCAGCT ACACGTTTCC CCCTCCAGTT
    ATTTCTCACA CTTCCCCAAC AAAGGTATGG AGAAATAAAG AGGGAACAAG TGCTTATCAA
    GTACAAGAAA CCCCAGTTTC TCAGGTCGTA AGAAAGAAGA CATCATCTTA TGTTGGACTA
    GTTCTTGTTC TCCTCAGAGG GCTTCCGGGA TCAGGAAAAT CTTTTTTGGC AAGGACTTTG
    CAAGAGGATA ATCCAAGTGG AATTATTCTT AGTACTGATG ATTATTTTTA CATAAATGGG
    CAGTATCAGT TTGATGTAAA GTACCTAGGA GAAGCACACG ACTGGAACCA GAATCGTGCA
    AAAGAAGCAT TTGAGAAGAA GGTGTCTCCT ATAATAATAG ATAATACAAA CCTACAGGCA
    TGGGAAATGA AGCCATATGT TTCTTTGTCT CAAAAACATA AATATAAAGT CCTTTTCCGG
    GAACCGGACA CGTGGTGGAA ATTCAAACCA AAGGAACTTG CGAGGCGTAA TATTCATGGG
    GTAAGCAAAG AAAAAATAAC AAGAATGTTG GAACATTATC AGCGTTTTGT TTCAGTGCCA
    ATAATTATGA GTTCTTCAGT TCCAGAGAAA ATTGAACGTG TTGCGTTGTG TGCATATTCT
    TGTGAGGACA GAAATACTAG CCCAAGAGAC AATGAAGATG TTACCTCTGA AAAAGAAGAA
    AATATTTTAT CCTCATCTTT GAAGCATCTA GAGTTAACTG AAGAGAAGAA ACTTGAAGTG
    GCCAAAGAAA CTATATTACC TGAGAATGTT ACATATCTCT CTAATGGAGA TTTAAACAAA
    GGAAGAACAG AAATAAGTGT TACGAATTCT CAGAATCAGA GTGCTTTTAT TCAGGAAGTT
    TCAGACGTGT ATTTTTCTGA TTCTGAAAGC AAAGTACAAG CCACAGAAAG TAAAAGAGAA
    GAGCAAGTAG AACTGGCTTC TGAAAAAGAA TATAGTAAAA CTGATGCAGA TAGTTTTGTA
    GAGAGAATGT CACTGAGTAC TTGCTGTGGT GAAAATAATC AAGAAGACTA TGATCTTTCA
    GATACTGTGC CATTTCAAAA TGAAAAATCT TCACCTGGTG AAACATTAGA AGAAAGAGCC
    ACAGTAAAGA AAAAAGCCTT TGGAAAACAA AAAAGCAAGT CAACTTTGGA AAAGTTCCCA
    AGACATGAGC TATCAAATTT TGTCGGTGAC TGGCCAGTTG ATAAGACTAT TGGTCAGAGG
    GCCAAAAGGA ACCGAAAAAC TGAAAAAGCT TCATCTATAC AAAGTGACAA AAAATATAAT
    CGCCCTCAGT CACGCAAAGT ATTAGATAAT ACTCTATCTG CAAATATAGA TCATATCCAG
    CAACGAGAAT CTCCAAATGA AAGTACAGAG GATGACAAGA AGTCACAGTA TGATGATGCT
    TCAGAATCTT TCAATTCCTG TCAATATGAT ACGTATAAAG ATACTGAAAA ACAGTCATTT
    AACATTGTGG GTGACTGGCC TTCATCTGAT TCCTTAGCTC AGAGAGAGCA CAGGTCAAGA
    ATGCCAAAGG ATGGTTTAAA GGAACCCAAC CTAGAATTTG GAACTAATGA CAGTATGAAT
    GAAATAGCCT TCTACATAGC ACATGAGACC TGTTGTTGGG GCACAAGCCC TGAAGAATTA
    AAAACCCTGG GTAGCTCCAC TCTAGGGAGT TCTGAAACAC TACCCAATAA AGTGACCTGT
    GAGAATAAGA CTTGTCCAAG TAAAAAGATT CATAGACAGC ACACATCGTC TCTCACTAGT
    ACCAACAGTG GGCCAACTTT TCCTGGAGTA GTAGGACCAC AAACTTCCGT TGAATTTCCA
    GAAGGAAAAC CTAATACAGT CCCTGGAATA GAAGAAGGCA TGTGTACCCA GACTGAACCA
    CAGGATTTTG CTCTCTTATG GAAAATAGAA AAGAATAAAA TTAATGTTTC AGATTCTGTG
    AGAGTGTTAA CAGGACAATT AGATGGATTT AAACCAAAAG CTTTCAATAT TAACACAAAG
    TTAGATGTTC AAGAAACAAT TCCATATAGG GTAATGTATG ATAAGAGCAC GTATGTTGAA
    GAAAGTGAGC TTACCAGTGC TGATGAATCT GAAAATCTTA ATATTCTTTG TAAACTCTTT
    GGGTCCTTTT CATTAGAAGC CCTAAAGGAC TTATATGAGA GGTGTAACAA AGATATTATT
    TGGGCCACAA GTCTCTTGTT GGATTCTGAA ACAAAATTAT GTGAGGATAC TGAATTTGAG
    AATATCCAAA AATCGTACGA TGAAGCACAA ATTGGGCCAT TTTCTCTGGG ATTAAATTTG
    AAGGAAATTA TTAGTCAAAG AGGAACTTTG GAAGATTCTA GTTCTGTGCC AGAATTTAGC
    CATGGCACTG CTGTTAATAA CCCTGATGTA CAATCTACCT GTGATTCAGA AGAGGGAAAC
    TCAGAGCAGG CAGAAATAAG AGCTATAACT ACTGAAAAAC CTGTGTTAAT AACAGGTTTA
    TTTCCTAATG CCACTCTAGA TCTAAAGAAT AATAATGAAG TACTTCCCAG TAGTCAGGTA
    GAACTTCCAG GTCTATATAA CATTAAGCAG TCTTTCCCAG GTACTCTAAA ACCAACTCTT
    GCTAAAGATA TGAGTGAAAT GGAGAAGAAT CTAGAAGCCA CGGAGATTGG AGACAGTATA
    CATTCTTCTT TGAATTTGTC TGATATTTTA AACTCTGTAT CAAGCACTTC AAATCTTGAA
    TTAAATGAAG AACTTGACTT TACTGACTCT CTTGAAATAA AGAAAAATGA AAATCTTCCA
    AAGGATTATA TGAGATTTCC AAGCACTGAA GAATTTATGA ATGAAGATGA ACAGGAAATG
    GAAAAAATTC TAATGGCTGA GAGTACTTTC TCAGCTGAAG TTAGTGAAGA AGATAAAACT
    GAGACGTTGA ATCCCATGCC AGTGATGGCC AAATCACTGA CCATAGACTG TCTGGAATTG
    GCATTACCCC CTGAGCTGGC TTTTCAGCTC AATGAATTAT TTGGCCCTGT TGGTATTGAT
    TCAGGGTCTC TAACAGTTGA GGATTGTGTG GTTCATATAG ATCTGAATCT GGCTAAAGTG
    ATTCATGAGA AATGGAAAGA ATCTATAATG GAGCGACAAA GACAAGAAGA GGTATCCTGT
    GGCAAGTTTA TGCAAGATCC TTCCCTGGTT GGGCATATTG GTCTTGATAA TCCAGAACAA
    AAATCATCTC AGAAAACAGG CAAAAAATTA CTGAAGACTT TAGCAGCACC TGAAATGCTA
    CCCTTATTGG ATCATTGGAA CGCTCAAACT AAAAAAGTCT CACTCAGAGA AATAATGTCA
    GAAGAAATTG CCTTACAGGA AAAACATGAT TTGAAAAGGG AGGCACTTAT GTTTGAAAAA
    GATTGTGCCA CTAAACTAAA GGAGAAGCAG CTCTTTAAGA TATTTCCAGC CATTAACCAA
    AATTTTCTGG TGGACATTTT CAAGGACCAC AACTATTCAT TAGAACACAC AGTTCAGTTT
    CTAAACTGTG TTCTTGAAGG AGACCCTGTA AAAACGGTCG TAGCGCAAGA GTTTGTTCAC
    CCAAATGAGA ATGTCACTTC TCATACTACA CAGAAGTCTA AGGAGAAAAA GGCAAAGAAA
    TCGAAAGAGA CTGAAGATAT ACCAAGTGAA CCATCTTTCC AGGATTTTGA GTACCCAGAG
    TACAATGACT ACAGGGCAGA GGCTTTTCTG CACCAACAGA AGAGGATGGA ATGCTACAGC
    AAAGCGAAAG AAGCTTATCG GATGGGGAAA AAACACGTTG CCACGTTTTA TGCCCAGCAG
    GGTAGTCTCC ATGAGCAGAA GATGAAAGAA GCCAATCACC TTGCTGCTGT GGAGATCTTT
    GAGAAAGTCA ATGCCTCCCT GCTGCCACAG AATGTTTTAG ACCTCCATGG GCTGCATGTG
    GATGAAGCTA TAGAACATTT GATGACAGTT TTACAGCAGA AGACTGAAGA ATTTAAGCAG
    AATGGTGGTA AGTCCTATCT CTCTGTGATT ACGGGGAGAG GAAACCACAG CCAAGGAGGA
    GTTGCACGCA TCAAACCAGC CGTCATTAAA TACCTCATAA GCCATAGCTT CAGGTTCTCT
    GAAATTAAAC CAGGGTGCTT GAAAGTCATG CTAAAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04567
    Clone ID Related Accession (Same CDS sequence) XM_005618609.2 , XM_005618610.2 , XM_014112634.1
    Accession Version XM_005618609.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618609.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGGCACT TATGTTTGAA
    AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC
    CAAAATTTTC TGGTGGACAT TTTCAAGGAC CACAACTATT CATTAGAACA CACAGTTCAG
    TTTCTAAACT GTGTTCTTGA AGGAGACCCT GTAAAAACGG TCGTAGCGCA AGAGTTTGTT
    CACCCAAATG AGAATGTCAC TTCTCATACT ACACAGAAGT CTAAGGAGAA AAAGGCAAAG
    AAATCGAAAG AGACTGAAGA TATACCAAGT GAACCATCTT TCCAGGATTT TGAGTACCCA
    GAGTACAATG ACTACAGGGC AGAGGCTTTT CTGCACCAAC AGAAGAGGAT GGAATGCTAC
    AGCAAAGCGA AAGAAGCTTA TCGGATGGGG AAAAAACACG TTGCCACGTT TTATGCCCAG
    CAGGGTAGTC TCCATGAGCA GAAGATGAAA GAAGCCAATC ACCTTGCTGC TGTGGAGATC
    TTTGAGAAAG TCAATGCCTC CCTGCTGCCA CAGAATGTTT TAGACCTCCA TGGGCTGCAT
    GTGGATGAAG CTATAGAACA TTTGATGACA GTTTTACAGC AGAAGACTGA AGAATTTAAG
    CAGAATGGTG GTAAGTCCTA TCTCTCTGTG ATTACGGGGA GAGGAAACCA CAGCCAAGGA
    GGAGTTGCAC GCATCAAACC AGCCGTCATT AAATACCTCA TAAGCCATAG CTTCAGGTTC
    TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618666.1
    CDS188..5269
    Translation

    Target ORF information:

    RefSeq Version XM_005618609.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618609.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGGCACT TATGTTTGAA
    AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC
    CAAAATTTTC TGGTGGACAT TTTCAAGGAC CACAACTATT CATTAGAACA CACAGTTCAG
    TTTCTAAACT GTGTTCTTGA AGGAGACCCT GTAAAAACGG TCGTAGCGCA AGAGTTTGTT
    CACCCAAATG AGAATGTCAC TTCTCATACT ACACAGAAGT CTAAGGAGAA AAAGGCAAAG
    AAATCGAAAG AGACTGAAGA TATACCAAGT GAACCATCTT TCCAGGATTT TGAGTACCCA
    GAGTACAATG ACTACAGGGC AGAGGCTTTT CTGCACCAAC AGAAGAGGAT GGAATGCTAC
    AGCAAAGCGA AAGAAGCTTA TCGGATGGGG AAAAAACACG TTGCCACGTT TTATGCCCAG
    CAGGGTAGTC TCCATGAGCA GAAGATGAAA GAAGCCAATC ACCTTGCTGC TGTGGAGATC
    TTTGAGAAAG TCAATGCCTC CCTGCTGCCA CAGAATGTTT TAGACCTCCA TGGGCTGCAT
    GTGGATGAAG CTATAGAACA TTTGATGACA GTTTTACAGC AGAAGACTGA AGAATTTAAG
    CAGAATGGTG GTAAGTCCTA TCTCTCTGTG ATTACGGGGA GAGGAAACCA CAGCCAAGGA
    GGAGTTGCAC GCATCAAACC AGCCGTCATT AAATACCTCA TAAGCCATAG CTTCAGGTTC
    TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04567
    Clone ID Related Accession (Same CDS sequence) XM_005618609.2 , XM_005618610.2 , XM_014112634.1
    Accession Version XM_005618610.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005618610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGGCACT TATGTTTGAA
    AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC
    CAAAATTTTC TGGTGGACAT TTTCAAGGAC CACAACTATT CATTAGAACA CACAGTTCAG
    TTTCTAAACT GTGTTCTTGA AGGAGACCCT GTAAAAACGG TCGTAGCGCA AGAGTTTGTT
    CACCCAAATG AGAATGTCAC TTCTCATACT ACACAGAAGT CTAAGGAGAA AAAGGCAAAG
    AAATCGAAAG AGACTGAAGA TATACCAAGT GAACCATCTT TCCAGGATTT TGAGTACCCA
    GAGTACAATG ACTACAGGGC AGAGGCTTTT CTGCACCAAC AGAAGAGGAT GGAATGCTAC
    AGCAAAGCGA AAGAAGCTTA TCGGATGGGG AAAAAACACG TTGCCACGTT TTATGCCCAG
    CAGGGTAGTC TCCATGAGCA GAAGATGAAA GAAGCCAATC ACCTTGCTGC TGTGGAGATC
    TTTGAGAAAG TCAATGCCTC CCTGCTGCCA CAGAATGTTT TAGACCTCCA TGGGCTGCAT
    GTGGATGAAG CTATAGAACA TTTGATGACA GTTTTACAGC AGAAGACTGA AGAATTTAAG
    CAGAATGGTG GTAAGTCCTA TCTCTCTGTG ATTACGGGGA GAGGAAACCA CAGCCAAGGA
    GGAGTTGCAC GCATCAAACC AGCCGTCATT AAATACCTCA TAAGCCATAG CTTCAGGTTC
    TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005618667.1
    CDS580..5661
    Translation

    Target ORF information:

    RefSeq Version XM_005618610.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005618610.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGGCACT TATGTTTGAA
    AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC
    CAAAATTTTC TGGTGGACAT TTTCAAGGAC CACAACTATT CATTAGAACA CACAGTTCAG
    TTTCTAAACT GTGTTCTTGA AGGAGACCCT GTAAAAACGG TCGTAGCGCA AGAGTTTGTT
    CACCCAAATG AGAATGTCAC TTCTCATACT ACACAGAAGT CTAAGGAGAA AAAGGCAAAG
    AAATCGAAAG AGACTGAAGA TATACCAAGT GAACCATCTT TCCAGGATTT TGAGTACCCA
    GAGTACAATG ACTACAGGGC AGAGGCTTTT CTGCACCAAC AGAAGAGGAT GGAATGCTAC
    AGCAAAGCGA AAGAAGCTTA TCGGATGGGG AAAAAACACG TTGCCACGTT TTATGCCCAG
    CAGGGTAGTC TCCATGAGCA GAAGATGAAA GAAGCCAATC ACCTTGCTGC TGTGGAGATC
    TTTGAGAAAG TCAATGCCTC CCTGCTGCCA CAGAATGTTT TAGACCTCCA TGGGCTGCAT
    GTGGATGAAG CTATAGAACA TTTGATGACA GTTTTACAGC AGAAGACTGA AGAATTTAAG
    CAGAATGGTG GTAAGTCCTA TCTCTCTGTG ATTACGGGGA GAGGAAACCA CAGCCAAGGA
    GGAGTTGCAC GCATCAAACC AGCCGTCATT AAATACCTCA TAAGCCATAG CTTCAGGTTC
    TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe04567
    Clone ID Related Accession (Same CDS sequence) XM_005618609.2 , XM_005618610.2 , XM_014112634.1
    Accession Version XM_014112634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product NEDD4-binding protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726054.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGGCACT TATGTTTGAA
    AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC
    CAAAATTTTC TGGTGGACAT TTTCAAGGAC CACAACTATT CATTAGAACA CACAGTTCAG
    TTTCTAAACT GTGTTCTTGA AGGAGACCCT GTAAAAACGG TCGTAGCGCA AGAGTTTGTT
    CACCCAAATG AGAATGTCAC TTCTCATACT ACACAGAAGT CTAAGGAGAA AAAGGCAAAG
    AAATCGAAAG AGACTGAAGA TATACCAAGT GAACCATCTT TCCAGGATTT TGAGTACCCA
    GAGTACAATG ACTACAGGGC AGAGGCTTTT CTGCACCAAC AGAAGAGGAT GGAATGCTAC
    AGCAAAGCGA AAGAAGCTTA TCGGATGGGG AAAAAACACG TTGCCACGTT TTATGCCCAG
    CAGGGTAGTC TCCATGAGCA GAAGATGAAA GAAGCCAATC ACCTTGCTGC TGTGGAGATC
    TTTGAGAAAG TCAATGCCTC CCTGCTGCCA CAGAATGTTT TAGACCTCCA TGGGCTGCAT
    GTGGATGAAG CTATAGAACA TTTGATGACA GTTTTACAGC AGAAGACTGA AGAATTTAAG
    CAGAATGGTG GTAAGTCCTA TCTCTCTGTG ATTACGGGGA GAGGAAACCA CAGCCAAGGA
    GGAGTTGCAC GCATCAAACC AGCCGTCATT AAATACCTCA TAAGCCATAG CTTCAGGTTC
    TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968109.1
    CDS673..5754
    Translation

    Target ORF information:

    RefSeq Version XM_014112634.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris NEDD4 binding protein 2 (N4BP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014112634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGATTGTC TATTAGAATT ATCTGCCACT GATGCCAAGG TAGAAGAATC ATCTTCAAAA 
    AGCTTTGTTG CCTCCGAGAA CCAAGTAAGT GCTGTGGGAC GTGAAATAAT GGAAAAATAT
    ACTCCTGAAG AAGAGAGGGA AGATTCAAAA ATGGATTCAT TTTTGGACAT GCAGCTAACT
    GAAGACTTGG ATTCCTTAAT ACAGAATGCT TTTGAGAAAT TGAACTCTTC TCCTGATGAC
    CAAGTATACT CATTTTTGCC TTTGCAAGGT GTTAATAGTT TTAGTGACCC GAGTGCATTT
    ATAAATTCAG ATTCAAGTAA TATGACTCCC ATTCTTTCTA CACAGAATAT GGATTTGGAC
    AGTGAAAATT TAGAGAATTC TGCCTCTGCG TTAAGTTCAA ACACCTTAAT TTCACATTCT
    GTTTCTGATG AGTCCAAAAA TTTTATAAAG GATGACGTAT TGGCATTGGA AGATCCTCTT
    CTCAGTAGTT CTTTAAATGT AGCAAATAAC ACTATGGTGA GTAGTAGCAA TCTCAGTCAA
    AGGCAGAAAG AGCTTTTAGA ATCTGAGTGT GTTGAGACTC CGTTCTCTCA AGCCCCTGTA
    GATTTGGATG CCAGTGAACC TCAGGCTCCT CTAAGCCTTA CTGCGCAAAA TTTGGGGCTT
    GATTTACTAG GTACAAGTGG GGATCAGAAA TCTGCCTCTG TCCCCGATGC ATTTGTACCT
    TCTGAAGAAT TCAGTTTCAA ACCACACAAA CATACTGAAC TTCCGTCAAA GGGGAAAGAT
    GTGAATTACT GTCCTGTACT TACCCCTCTC CCTCTGCTGC TGCCTCCTCC TCCACCTCCA
    CCAATGTGGA ATCCAATGAT TCCTGCTTTT GACCTCTTTC AAGGAAATCA TGGCTTTATA
    GCTCCTGTTG TAACCACCGC TACGCACTGG AGACCGGTCA GCTACACGTT TCCCCCTCCA
    GTTATTTCTC ACACTTCCCC AACAAAGGTA TGGAGAAATA AAGAGGGAAC AAGTGCTTAT
    CAAGTACAAG AAACCCCAGT TTCTCAGGTC GTAAGAAAGA AGACATCATC TTATGTTGGA
    CTAGTTCTTG TTCTCCTCAG AGGGCTTCCG GGATCAGGAA AATCTTTTTT GGCAAGGACT
    TTGCAAGAGG ATAATCCAAG TGGAATTATT CTTAGTACTG ATGATTATTT TTACATAAAT
    GGGCAGTATC AGTTTGATGT AAAGTACCTA GGAGAAGCAC ACGACTGGAA CCAGAATCGT
    GCAAAAGAAG CATTTGAGAA GAAGGTGTCT CCTATAATAA TAGATAATAC AAACCTACAG
    GCATGGGAAA TGAAGCCATA TGTTTCTTTG TCTCAAAAAC ATAAATATAA AGTCCTTTTC
    CGGGAACCGG ACACGTGGTG GAAATTCAAA CCAAAGGAAC TTGCGAGGCG TAATATTCAT
    GGGGTAAGCA AAGAAAAAAT AACAAGAATG TTGGAACATT ATCAGCGTTT TGTTTCAGTG
    CCAATAATTA TGAGTTCTTC AGTTCCAGAG AAAATTGAAC GTGTTGCGTT GTGTGCATAT
    TCTTGTGAGG ACAGAAATAC TAGCCCAAGA GACAATGAAG ATGTTACCTC TGAAAAAGAA
    GAAAATATTT TATCCTCATC TTTGAAGCAT CTAGAGTTAA CTGAAGAGAA GAAACTTGAA
    GTGGCCAAAG AAACTATATT ACCTGAGAAT GTTACATATC TCTCTAATGG AGATTTAAAC
    AAAGGAAGAA CAGAAATAAG TGTTACGAAT TCTCAGAATC AGAGTGCTTT TATTCAGGAA
    GTTTCAGACG TGTATTTTTC TGATTCTGAA AGCAAAGTAC AAGCCACAGA AAGTAAAAGA
    GAAGAGCAAG TAGAACTGGC TTCTGAAAAA GAATATAGTA AAACTGATGC AGATAGTTTT
    GTAGAGAGAA TGTCACTGAG TACTTGCTGT GGTGAAAATA ATCAAGAAGA CTATGATCTT
    TCAGATACTG TGCCATTTCA AAATGAAAAA TCTTCACCTG GTGAAACATT AGAAGAAAGA
    GCCACAGTAA AGAAAAAAGC CTTTGGAAAA CAAAAAAGCA AGTCAACTTT GGAAAAGTTC
    CCAAGACATG AGCTATCAAA TTTTGTCGGT GACTGGCCAG TTGATAAGAC TATTGGTCAG
    AGGGCCAAAA GGAACCGAAA AACTGAAAAA GCTTCATCTA TACAAAGTGA CAAAAAATAT
    AATCGCCCTC AGTCACGCAA AGTATTAGAT AATACTCTAT CTGCAAATAT AGATCATATC
    CAGCAACGAG AATCTCCAAA TGAAAGTACA GAGGATGACA AGAAGTCACA GTATGATGAT
    GCTTCAGAAT CTTTCAATTC CTGTCAATAT GATACGTATA AAGATACTGA AAAACAGTCA
    TTTAACATTG TGGGTGACTG GCCTTCATCT GATTCCTTAG CTCAGAGAGA GCACAGGTCA
    AGAATGCCAA AGGATGGTTT AAAGGAACCC AACCTAGAAT TTGGAACTAA TGACAGTATG
    AATGAAATAG CCTTCTACAT AGCACATGAG ACCTGTTGTT GGGGCACAAG CCCTGAAGAA
    TTAAAAACCC TGGGTAGCTC CACTCTAGGG AGTTCTGAAA CACTACCCAA TAAAGTGACC
    TGTGAGAATA AGACTTGTCC AAGTAAAAAG ATTCATAGAC AGCACACATC GTCTCTCACT
    AGTACCAACA GTGGGCCAAC TTTTCCTGGA GTAGTAGGAC CACAAACTTC CGTTGAATTT
    CCAGAAGGAA AACCTAATAC AGTCCCTGGA ATAGAAGAAG GCATGTGTAC CCAGACTGAA
    CCACAGGATT TTGCTCTCTT ATGGAAAATA GAAAAGAATA AAATTAATGT TTCAGATTCT
    GTGAGAGTGT TAACAGGACA ATTAGATGGA TTTAAACCAA AAGCTTTCAA TATTAACACA
    AAGTTAGATG TTCAAGAAAC AATTCCATAT AGGGTAATGT ATGATAAGAG CACGTATGTT
    GAAGAAAGTG AGCTTACCAG TGCTGATGAA TCTGAAAATC TTAATATTCT TTGTAAACTC
    TTTGGGTCCT TTTCATTAGA AGCCCTAAAG GACTTATATG AGAGGTGTAA CAAAGATATT
    ATTTGGGCCA CAAGTCTCTT GTTGGATTCT GAAACAAAAT TATGTGAGGA TACTGAATTT
    GAGAATATCC AAAAATCGTA CGATGAAGCA CAAATTGGGC CATTTTCTCT GGGATTAAAT
    TTGAAGGAAA TTATTAGTCA AAGAGGAACT TTGGAAGATT CTAGTTCTGT GCCAGAATTT
    AGCCATGGCA CTGCTGTTAA TAACCCTGAT GTACAATCTA CCTGTGATTC AGAAGAGGGA
    AACTCAGAGC AGGCAGAAAT AAGAGCTATA ACTACTGAAA AACCTGTGTT AATAACAGGT
    TTATTTCCTA ATGCCACTCT AGATCTAAAG AATAATAATG AAGTACTTCC CAGTAGTCAG
    GTAGAACTTC CAGGTCTATA TAACATTAAG CAGTCTTTCC CAGGTACTCT AAAACCAACT
    CTTGCTAAAG ATATGAGTGA AATGGAGAAG AATCTAGAAG CCACGGAGAT TGGAGACAGT
    ATACATTCTT CTTTGAATTT GTCTGATATT TTAAACTCTG TATCAAGCAC TTCAAATCTT
    GAATTAAATG AAGAACTTGA CTTTACTGAC TCTCTTGAAA TAAAGAAAAA TGAAAATCTT
    CCAAAGGATT ATATGAGATT TCCAAGCACT GAAGAATTTA TGAATGAAGA TGAACAGGAA
    ATGGAAAAAA TTCTAATGGC TGAGAGTACT TTCTCAGCTG AAGTTAGTGA AGAAGATAAA
    ACTGAGACGT TGAATCCCAT GCCAGTGATG GCCAAATCAC TGACCATAGA CTGTCTGGAA
    TTGGCATTAC CCCCTGAGCT GGCTTTTCAG CTCAATGAAT TATTTGGCCC TGTTGGTATT
    GATTCAGGGT CTCTAACAGT TGAGGATTGT GTGGTTCATA TAGATCTGAA TCTGGCTAAA
    GTGATTCATG AGAAATGGAA AGAATCTATA ATGGAGCGAC AAAGACAAGA AGAGGTATCC
    TGTGGCAAGT TTATGCAAGA TCCTTCCCTG GTTGGGCATA TTGGTCTTGA TAATCCAGAA
    CAAAAATCAT CTCAGAAAAC AGGCAAAAAA TTACTGAAGA CTTTAGCAGC ACCTGAAATG
    CTACCCTTAT TGGATCATTG GAACGCTCAA ACTAAAAAAG TCTCACTCAG AGAAATAATG
    TCAGAAGAAA TTGCCTTACA GGAAAAACAT GATTTGAAAA GGGAGGCACT TATGTTTGAA
    AAAGATTGTG CCACTAAACT AAAGGAGAAG CAGCTCTTTA AGATATTTCC AGCCATTAAC
    CAAAATTTTC TGGTGGACAT TTTCAAGGAC CACAACTATT CATTAGAACA CACAGTTCAG
    TTTCTAAACT GTGTTCTTGA AGGAGACCCT GTAAAAACGG TCGTAGCGCA AGAGTTTGTT
    CACCCAAATG AGAATGTCAC TTCTCATACT ACACAGAAGT CTAAGGAGAA AAAGGCAAAG
    AAATCGAAAG AGACTGAAGA TATACCAAGT GAACCATCTT TCCAGGATTT TGAGTACCCA
    GAGTACAATG ACTACAGGGC AGAGGCTTTT CTGCACCAAC AGAAGAGGAT GGAATGCTAC
    AGCAAAGCGA AAGAAGCTTA TCGGATGGGG AAAAAACACG TTGCCACGTT TTATGCCCAG
    CAGGGTAGTC TCCATGAGCA GAAGATGAAA GAAGCCAATC ACCTTGCTGC TGTGGAGATC
    TTTGAGAAAG TCAATGCCTC CCTGCTGCCA CAGAATGTTT TAGACCTCCA TGGGCTGCAT
    GTGGATGAAG CTATAGAACA TTTGATGACA GTTTTACAGC AGAAGACTGA AGAATTTAAG
    CAGAATGGTG GTAAGTCCTA TCTCTCTGTG ATTACGGGGA GAGGAAACCA CAGCCAAGGA
    GGAGTTGCAC GCATCAAACC AGCCGTCATT AAATACCTCA TAAGCCATAG CTTCAGGTTC
    TCTGAAATTA AACCAGGGTG CTTGAAAGTC ATGCTAAAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.