PLCL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLCL1
Entrez Gene ID	478858
Full Name	phospholipase C like 1 (inactive)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014110960.1	XP_013966435.1	inactive phospholipase C-like protein 1 isoform X2
XM_536020.6	XP_536020.4	inactive phospholipase C-like protein 1 isoform X1
XM_005640502.3	XP_005640559.1	inactive phospholipase C-like protein 1 isoform X3
XM_005640502.2	XP_005640559.1	inactive phospholipase C-like protein 1 isoform X3
XM_536020.5	XP_536020.4	inactive phospholipase C-like protein 1 isoform X1
XM_022415139.1	XP_022270847.1	inactive phospholipase C-like protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLCL1	XP_001169560.1
	PLCL1	XP_001089483.1
Canis lupus familiaris (dog)	PLCL1	XP_536020.4
Rattus norvegicus (Norway rat)	Plcl1	NP_445908.1
Bos taurus (cattle)	PLCL1	NP_001178208.1
Xenopus tropicalis (tropical clawed frog)	plcl1	XP_002937769.2
Gallus gallus (chicken)	PLCL1	XP_421916.3
Mus musculus (house mouse)	Plcl1	NP_001108135.1
Homo sapiens (human)	PLCL1	NP_006217.3

Gene Ontology

The following PLCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe31327	XM_005640502.3 Latest version!	Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe45183	XM_014110960.1 Latest version!	Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe284877	XM_022415139.1 Latest version!	Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OCe31326	XM_536020.6 Latest version!	Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OCe31327	XM_005640502.2	Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OCe31326	XM_536020.5	Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLCL1 ( XM_005640502.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640559.1 Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe31327
Clone ID Related Accession (Same CDS sequence)	XM_005640502.3 , XM_005640502.2
Accession Version	XM_005640502.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive phospholipase C-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG AAAAGAAAAT TAGCAGTGCG AGTGACTGCA TCAGCTTCAT GCAAGCTGGC 
TGTGAACTGA AGAAGGTCCG GCCAAATTCT CGTATTTATA ACCGTTTTTT CACTCTGGAC 
ACAGACCTCC AAGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTTGAGAA AGCTAAGCTT 
GACATTTCCG CTATAAAAGA GATCAGACTG GGGAAGAACA CAGAAACATT TAGAAACAAT 
GGCCTCGCTG ACCAGATTTG TGAGGACTGT GCCTTTTCCA TACTCCATGG GGAAAACTAT 
GAGTCTCTGG ACCTAGTTGC CAATTCAGCA GACGTAGCAA ACATCTGGGT GTCAGGTTTA 
CGGTACCTGG TTTCTCGAAG TAAGCAACCC CTAGACTTTA TGGAGGGCAA TCAGAACACA 
CCAAGATTCA TGTGGTTGAA AACGGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAGCTCA ACCCTACCCT GAAGGAATCC 
AAGATCAGGT TAAAGTTTAA AGAAATCCAG AAGAGCAAAG AAAAACTAAC CACACGAGTG 
ACAGAAGAGG AATTTTGTGA AGCTTTTTGT GAACTCTGCA CCAGGCCAGA AGTATATTTC 
TTACTGGTAC AGATATCTAA AAACAAAGAA TATTTGGATG CCAACGATCT CATGCTCTTT 
TTAGAAGCTG AGCAAGGAGT CGCCCATATT ACTGAGGATA TGTGCTTAGA CATTATTCGG 
AGATACGAAC TTTCCGAAGA GGGACGTCAA AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATCTGT TGTCACCAGA ATGTGACATT TTTGATCCTG AGCAGAAAAA GGTTGCTCAA 
GATATGACCC AGCCATTATC TCACTACTAC ATCAATGCAT CTCACAACAC CTATCTCATA 
GAAGACCAGT TCAGGGGCCC GGCTGATATT AATGGATATG TTAGAGCTTT GAAAATGGGC 
TGTCGTAGCA TTGAACTTGA TGTCAGTGAT GGTTCAGACA ATGAACCGAT CCTTTGTAAT 
CGAAATAATG TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTTG CTTCTGAGTA CCCACTCATT CTTTGTTTGG GAAATCACTG CTCCCTACCA 
CAGCAGAAGA TAATGGTTCA ACAGATGAAA AAGGTCTTTG GCGATAAGCT CTATGTAGAG 
GCGCCTTTGC CATCAGAAAC CTACCTCCCA TCACCAGAAA AATTGAAAAG AATGATTATT 
GTGAAAGGAA AGAAATTGCC TTCTGACCCG GATGTTTTAG AAGGAGAAGT AACAGATGAA 
GATGAAGAAG CGGAAATGTC TCGAAGGCTG TCGGTAGATT ACAATGGTGA GCAGAAACAA 
ATCTTGCTGT GTAAGGAGCT CTCTGATTTG GTATCTATCT GCAAGTCTGT TCAGTACAGG 
GATTTTGAAC TGTCTATGAA GAGCCAAAAC TATTGGGAAA TGTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAGGTTC 
TTATCAAGGG TCTATCCAAG TGCCATGAGG ATCGATTCCA GTAACTTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GATCTTCACA CAGGCTGGTT TCTTCAAAAT GGAGGATGTG GTTATGTTCT ACGGCCATCC 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG CTCTTCATAT CAAGATCATC AGTGGTCAGA ATTTTCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGAGATGT CATAGATCCC TATGTTTGTA TAGAGATACA TGGAATTCCA 
GCAGATTGTT CAGAACAAAG AACTAAAACT GTCCAGCAGA ACAGTGATAA CCCTATTTTT 
GATGAAACTT TTGAGTTTCA AGTAAACCTA CCTGAGCTGG CCATGATTCG TTTTGTTGTT 
CTGGATGATG ACTACATAGG GGATGAGTTT ATAGGACAAT ATACAATACC ATTTGAATGT 
TTGCAGCCTG GATATCGGCA TGTGCCCCTG CGCTCTTTTG TGGGTGACAT CATGGAACAT 
GTAACCCTTT TTGTCCATAT AGCAATAACT AATCGAAGTG GAGGAGGAAA GGCACAGAAG 
CGCAGCCTGT CAGTGAGAAT GGGGAAGAAA GTTCGAGAAT ATACCATGCT CAGGAATATC 
GGCCTTAAGA CCATAGATGA CATCTTTAAA ATAGCAGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTATTA AGGAACTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCGT CTCGGCTCAT CACCAGTGAC 
AATACCCCTA CGGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTGGA GCCTCTGGGG 
GCAATTCCAG ATGTGCAGAA AAAAATGTTG GCTGCTTATG ACCTGATGAT TCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGATACAGTC CAGGAAAAGA TTGTGCAGTG TCAGAAATCA 
GGGATGGAAT TCCATGAGGA ACTTCATAAT CTAGGGGCAA AAGAAGGCTT GAAAGGAAGA 
AAACTCAACA AGGCAACTGA GAGCTTTGCT TGGAACATTA CAGTACTGAA GGGCCAAGGA 
GATCTGTTGA AGAACGCCAA GAATGAAGCT GTAGAAAACA TGAAGCAGAT CCAGCTGGCA 
TGCTTGTCTT GTGGACTTAG TAAAGCACCC AGCAGTGGTG CAGAAGCCAA GAGCAAACGC 
AGCCTGGAAG CCATAGAGGA GAAGGAGAGT GGCGAGGAGA ACGGGAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640559.1
CDS	132..3125
Translation	MPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVAHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVSDGSDNEPILCNRNNVTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKIMVQQMKKVFGDKLYVEAPLPSETYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRLSVDYNGEQKQILLCKELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKRFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPTVSLVMKDNFPYLEPLGAIPDVQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKSGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAVENMKQIQLACLSCGLSKAPSSGAEAKSKRSLEAIEEKESGEENGKL

Target ORF information:

RefSeq Version	XM_005640502.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640502.3

ORF Insert Sequence:

ATGCCCTCGG AAAAGAAAAT TAGCAGTGCG AGTGACTGCA TCAGCTTCAT GCAAGCTGGC 
TGTGAACTGA AGAAGGTCCG GCCAAATTCT CGTATTTATA ACCGTTTTTT CACTCTGGAC 
ACAGACCTCC AAGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTTGAGAA AGCTAAGCTT 
GACATTTCCG CTATAAAAGA GATCAGACTG GGGAAGAACA CAGAAACATT TAGAAACAAT 
GGCCTCGCTG ACCAGATTTG TGAGGACTGT GCCTTTTCCA TACTCCATGG GGAAAACTAT 
GAGTCTCTGG ACCTAGTTGC CAATTCAGCA GACGTAGCAA ACATCTGGGT GTCAGGTTTA 
CGGTACCTGG TTTCTCGAAG TAAGCAACCC CTAGACTTTA TGGAGGGCAA TCAGAACACA 
CCAAGATTCA TGTGGTTGAA AACGGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAGCTCA ACCCTACCCT GAAGGAATCC 
AAGATCAGGT TAAAGTTTAA AGAAATCCAG AAGAGCAAAG AAAAACTAAC CACACGAGTG 
ACAGAAGAGG AATTTTGTGA AGCTTTTTGT GAACTCTGCA CCAGGCCAGA AGTATATTTC 
TTACTGGTAC AGATATCTAA AAACAAAGAA TATTTGGATG CCAACGATCT CATGCTCTTT 
TTAGAAGCTG AGCAAGGAGT CGCCCATATT ACTGAGGATA TGTGCTTAGA CATTATTCGG 
AGATACGAAC TTTCCGAAGA GGGACGTCAA AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATCTGT TGTCACCAGA ATGTGACATT TTTGATCCTG AGCAGAAAAA GGTTGCTCAA 
GATATGACCC AGCCATTATC TCACTACTAC ATCAATGCAT CTCACAACAC CTATCTCATA 
GAAGACCAGT TCAGGGGCCC GGCTGATATT AATGGATATG TTAGAGCTTT GAAAATGGGC 
TGTCGTAGCA TTGAACTTGA TGTCAGTGAT GGTTCAGACA ATGAACCGAT CCTTTGTAAT 
CGAAATAATG TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTTG CTTCTGAGTA CCCACTCATT CTTTGTTTGG GAAATCACTG CTCCCTACCA 
CAGCAGAAGA TAATGGTTCA ACAGATGAAA AAGGTCTTTG GCGATAAGCT CTATGTAGAG 
GCGCCTTTGC CATCAGAAAC CTACCTCCCA TCACCAGAAA AATTGAAAAG AATGATTATT 
GTGAAAGGAA AGAAATTGCC TTCTGACCCG GATGTTTTAG AAGGAGAAGT AACAGATGAA 
GATGAAGAAG CGGAAATGTC TCGAAGGCTG TCGGTAGATT ACAATGGTGA GCAGAAACAA 
ATCTTGCTGT GTAAGGAGCT CTCTGATTTG GTATCTATCT GCAAGTCTGT TCAGTACAGG 
GATTTTGAAC TGTCTATGAA GAGCCAAAAC TATTGGGAAA TGTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAGGTTC 
TTATCAAGGG TCTATCCAAG TGCCATGAGG ATCGATTCCA GTAACTTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GATCTTCACA CAGGCTGGTT TCTTCAAAAT GGAGGATGTG GTTATGTTCT ACGGCCATCC 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG CTCTTCATAT CAAGATCATC AGTGGTCAGA ATTTTCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGAGATGT CATAGATCCC TATGTTTGTA TAGAGATACA TGGAATTCCA 
GCAGATTGTT CAGAACAAAG AACTAAAACT GTCCAGCAGA ACAGTGATAA CCCTATTTTT 
GATGAAACTT TTGAGTTTCA AGTAAACCTA CCTGAGCTGG CCATGATTCG TTTTGTTGTT 
CTGGATGATG ACTACATAGG GGATGAGTTT ATAGGACAAT ATACAATACC ATTTGAATGT 
TTGCAGCCTG GATATCGGCA TGTGCCCCTG CGCTCTTTTG TGGGTGACAT CATGGAACAT 
GTAACCCTTT TTGTCCATAT AGCAATAACT AATCGAAGTG GAGGAGGAAA GGCACAGAAG 
CGCAGCCTGT CAGTGAGAAT GGGGAAGAAA GTTCGAGAAT ATACCATGCT CAGGAATATC 
GGCCTTAAGA CCATAGATGA CATCTTTAAA ATAGCAGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTATTA AGGAACTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCGT CTCGGCTCAT CACCAGTGAC 
AATACCCCTA CGGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTGGA GCCTCTGGGG 
GCAATTCCAG ATGTGCAGAA AAAAATGTTG GCTGCTTATG ACCTGATGAT TCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGATACAGTC CAGGAAAAGA TTGTGCAGTG TCAGAAATCA 
GGGATGGAAT TCCATGAGGA ACTTCATAAT CTAGGGGCAA AAGAAGGCTT GAAAGGAAGA 
AAACTCAACA AGGCAACTGA GAGCTTTGCT TGGAACATTA CAGTACTGAA GGGCCAAGGA 
GATCTGTTGA AGAACGCCAA GAATGAAGCT GTAGAAAACA TGAAGCAGAT CCAGCTGGCA 
TGCTTGTCTT GTGGACTTAG TAAAGCACCC AGCAGTGGTG CAGAAGCCAA GAGCAAACGC 
AGCCTGGAAG CCATAGAGGA GAAGGAGAGT GGCGAGGAGA ACGGGAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_005640502.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640559.1 Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe31327
Clone ID Related Accession (Same CDS sequence)	XM_005640502.3 , XM_005640502.2
Accession Version	XM_005640502.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive phospholipase C-like protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640502.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCGG AAAAGAAAAT TAGCAGTGCG AGTGACTGCA TCAGCTTCAT GCAAGCTGGC 
TGTGAACTGA AGAAGGTCCG GCCAAATTCT CGTATTTATA ACCGTTTTTT CACTCTGGAC 
ACAGACCTCC AAGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTTGAGAA AGCTAAGCTT 
GACATTTCCG CTATAAAAGA GATCAGACTG GGGAAGAACA CAGAAACATT TAGAAACAAT 
GGCCTCGCTG ACCAGATTTG TGAGGACTGT GCCTTTTCCA TACTCCATGG GGAAAACTAT 
GAGTCTCTGG ACCTAGTTGC CAATTCAGCA GACGTAGCAA ACATCTGGGT GTCAGGTTTA 
CGGTACCTGG TTTCTCGAAG TAAGCAACCC CTAGACTTTA TGGAGGGCAA TCAGAACACA 
CCAAGATTCA TGTGGTTGAA AACGGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAGCTCA ACCCTACCCT GAAGGAATCC 
AAGATCAGGT TAAAGTTTAA AGAAATCCAG AAGAGCAAAG AAAAACTAAC CACACGAGTG 
ACAGAAGAGG AATTTTGTGA AGCTTTTTGT GAACTCTGCA CCAGGCCAGA AGTATATTTC 
TTACTGGTAC AGATATCTAA AAACAAAGAA TATTTGGATG CCAACGATCT CATGCTCTTT 
TTAGAAGCTG AGCAAGGAGT CGCCCATATT ACTGAGGATA TGTGCTTAGA CATTATTCGG 
AGATACGAAC TTTCCGAAGA GGGACGTCAA AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATCTGT TGTCACCAGA ATGTGACATT TTTGATCCTG AGCAGAAAAA GGTTGCTCAA 
GATATGACCC AGCCATTATC TCACTACTAC ATCAATGCAT CTCACAACAC CTATCTCATA 
GAAGACCAGT TCAGGGGCCC GGCTGATATT AATGGATATG TTAGAGCTTT GAAAATGGGC 
TGTCGTAGCA TTGAACTTGA TGTCAGTGAT GGTTCAGACA ATGAACCGAT CCTTTGTAAT 
CGAAATAATG TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTTG CTTCTGAGTA CCCACTCATT CTTTGTTTGG GAAATCACTG CTCCCTACCA 
CAGCAGAAGA TAATGGTTCA ACAGATGAAA AAGGTCTTTG GCGATAAGCT CTATGTAGAG 
GCGCCTTTGC CATCAGAAAC CTACCTCCCA TCACCAGAAA AATTGAAAAG AATGATTATT 
GTGAAAGGAA AGAAATTGCC TTCTGACCCG GATGTTTTAG AAGGAGAAGT AACAGATGAA 
GATGAAGAAG CGGAAATGTC TCGAAGGCTG TCGGTAGATT ACAATGGTGA GCAGAAACAA 
ATCTTGCTGT GTAAGGAGCT CTCTGATTTG GTATCTATCT GCAAGTCTGT TCAGTACAGG 
GATTTTGAAC TGTCTATGAA GAGCCAAAAC TATTGGGAAA TGTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAGGTTC 
TTATCAAGGG TCTATCCAAG TGCCATGAGG ATCGATTCCA GTAACTTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GATCTTCACA CAGGCTGGTT TCTTCAAAAT GGAGGATGTG GTTATGTTCT ACGGCCATCC 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG CTCTTCATAT CAAGATCATC AGTGGTCAGA ATTTTCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGAGATGT CATAGATCCC TATGTTTGTA TAGAGATACA TGGAATTCCA 
GCAGATTGTT CAGAACAAAG AACTAAAACT GTCCAGCAGA ACAGTGATAA CCCTATTTTT 
GATGAAACTT TTGAGTTTCA AGTAAACCTA CCTGAGCTGG CCATGATTCG TTTTGTTGTT 
CTGGATGATG ACTACATAGG GGATGAGTTT ATAGGACAAT ATACAATACC ATTTGAATGT 
TTGCAGCCTG GATATCGGCA TGTGCCCCTG CGCTCTTTTG TGGGTGACAT CATGGAACAT 
GTAACCCTTT TTGTCCATAT AGCAATAACT AATCGAAGTG GAGGAGGAAA GGCACAGAAG 
CGCAGCCTGT CAGTGAGAAT GGGGAAGAAA GTTCGAGAAT ATACCATGCT CAGGAATATC 
GGCCTTAAGA CCATAGATGA CATCTTTAAA ATAGCAGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTATTA AGGAACTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCGT CTCGGCTCAT CACCAGTGAC 
AATACCCCTA CGGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTGGA GCCTCTGGGG 
GCAATTCCAG ATGTGCAGAA AAAAATGTTG GCTGCTTATG ACCTGATGAT TCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGATACAGTC CAGGAAAAGA TTGTGCAGTG TCAGAAATCA 
GGGATGGAAT TCCATGAGGA ACTTCATAAT CTAGGGGCAA AAGAAGGCTT GAAAGGAAGA 
AAACTCAACA AGGCAACTGA GAGCTTTGCT TGGAACATTA CAGTACTGAA GGGCCAAGGA 
GATCTGTTGA AGAACGCCAA GAATGAAGCT GTAGAAAACA TGAAGCAGAT CCAGCTGGCA 
TGCTTGTCTT GTGGACTTAG TAAAGCACCC AGCAGTGGTG CAGAAGCCAA GAGCAAACGC 
AGCCTGGAAG CCATAGAGGA GAAGGAGAGT GGCGAGGAGA ACGGGAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640559.1
CDS	141..3134
Translation	MPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVAHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVSDGSDNEPILCNRNNVTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKIMVQQMKKVFGDKLYVEAPLPSETYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRLSVDYNGEQKQILLCKELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKRFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPTVSLVMKDNFPYLEPLGAIPDVQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKSGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAVENMKQIQLACLSCGLSKAPSSGAEAKSKRSLEAIEEKESGEENGKL

Target ORF information:

RefSeq Version	XM_005640502.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640502.2

ORF Insert Sequence:

ATGCCCTCGG AAAAGAAAAT TAGCAGTGCG AGTGACTGCA TCAGCTTCAT GCAAGCTGGC 
TGTGAACTGA AGAAGGTCCG GCCAAATTCT CGTATTTATA ACCGTTTTTT CACTCTGGAC 
ACAGACCTCC AAGCTCTTCG CTGGGAACCT TCCAAGAAAG ACCTTGAGAA AGCTAAGCTT 
GACATTTCCG CTATAAAAGA GATCAGACTG GGGAAGAACA CAGAAACATT TAGAAACAAT 
GGCCTCGCTG ACCAGATTTG TGAGGACTGT GCCTTTTCCA TACTCCATGG GGAAAACTAT 
GAGTCTCTGG ACCTAGTTGC CAATTCAGCA GACGTAGCAA ACATCTGGGT GTCAGGTTTA 
CGGTACCTGG TTTCTCGAAG TAAGCAACCC CTAGACTTTA TGGAGGGCAA TCAGAACACA 
CCAAGATTCA TGTGGTTGAA AACGGTGTTT GAAGCAGCAG ATATCGATGG GAATGGGATT 
ATGTTGGAAG ACACCTCTGT AGAGTTAATA AAACAGCTCA ACCCTACCCT GAAGGAATCC 
AAGATCAGGT TAAAGTTTAA AGAAATCCAG AAGAGCAAAG AAAAACTAAC CACACGAGTG 
ACAGAAGAGG AATTTTGTGA AGCTTTTTGT GAACTCTGCA CCAGGCCAGA AGTATATTTC 
TTACTGGTAC AGATATCTAA AAACAAAGAA TATTTGGATG CCAACGATCT CATGCTCTTT 
TTAGAAGCTG AGCAAGGAGT CGCCCATATT ACTGAGGATA TGTGCTTAGA CATTATTCGG 
AGATACGAAC TTTCCGAAGA GGGACGTCAA AAAGGGTTTC TTGCAATTGA TGGCTTTACC 
CAGTATCTGT TGTCACCAGA ATGTGACATT TTTGATCCTG AGCAGAAAAA GGTTGCTCAA 
GATATGACCC AGCCATTATC TCACTACTAC ATCAATGCAT CTCACAACAC CTATCTCATA 
GAAGACCAGT TCAGGGGCCC GGCTGATATT AATGGATATG TTAGAGCTTT GAAAATGGGC 
TGTCGTAGCA TTGAACTTGA TGTCAGTGAT GGTTCAGACA ATGAACCGAT CCTTTGTAAT 
CGAAATAATG TGACAACACA CCTTTCCTTT CGAAGTGTCA TAGAGGTGAT AAATAAATTT 
GCCTTTGTTG CTTCTGAGTA CCCACTCATT CTTTGTTTGG GAAATCACTG CTCCCTACCA 
CAGCAGAAGA TAATGGTTCA ACAGATGAAA AAGGTCTTTG GCGATAAGCT CTATGTAGAG 
GCGCCTTTGC CATCAGAAAC CTACCTCCCA TCACCAGAAA AATTGAAAAG AATGATTATT 
GTGAAAGGAA AGAAATTGCC TTCTGACCCG GATGTTTTAG AAGGAGAAGT AACAGATGAA 
GATGAAGAAG CGGAAATGTC TCGAAGGCTG TCGGTAGATT ACAATGGTGA GCAGAAACAA 
ATCTTGCTGT GTAAGGAGCT CTCTGATTTG GTATCTATCT GCAAGTCTGT TCAGTACAGG 
GATTTTGAAC TGTCTATGAA GAGCCAAAAC TATTGGGAAA TGTGTTCATT TAGTGAAACA 
GAGGCCAGCC GAATTGCAAA TGAATACCCA GAGGATTTTG TAAATTATAA TAAGAGGTTC 
TTATCAAGGG TCTATCCAAG TGCCATGAGG ATCGATTCCA GTAACTTGAA TCCACAGGAC 
TTTTGGAATT GTGGCTGTCA GATTGTGGCA ATGAATTTTC AGACTCCAGG TCCAATGATG 
GATCTTCACA CAGGCTGGTT TCTTCAAAAT GGAGGATGTG GTTATGTTCT ACGGCCATCC 
ATCATGCGAG ATGAAGTTTC TTACTTCAGT GCAAACACAA AGGGCATTGT ACCTGGGGTG 
TCTCCTCTAG CTCTTCATAT CAAGATCATC AGTGGTCAGA ATTTTCCAAA GCCCAAGGGA 
GCTTGTGCCA AAGGAGATGT CATAGATCCC TATGTTTGTA TAGAGATACA TGGAATTCCA 
GCAGATTGTT CAGAACAAAG AACTAAAACT GTCCAGCAGA ACAGTGATAA CCCTATTTTT 
GATGAAACTT TTGAGTTTCA AGTAAACCTA CCTGAGCTGG CCATGATTCG TTTTGTTGTT 
CTGGATGATG ACTACATAGG GGATGAGTTT ATAGGACAAT ATACAATACC ATTTGAATGT 
TTGCAGCCTG GATATCGGCA TGTGCCCCTG CGCTCTTTTG TGGGTGACAT CATGGAACAT 
GTAACCCTTT TTGTCCATAT AGCAATAACT AATCGAAGTG GAGGAGGAAA GGCACAGAAG 
CGCAGCCTGT CAGTGAGAAT GGGGAAGAAA GTTCGAGAAT ATACCATGCT CAGGAATATC 
GGCCTTAAGA CCATAGATGA CATCTTTAAA ATAGCAGTTC ATCCCTTACG AGAAGCCATA 
GATATGAGAG AAAATATGCA GAATGCCATA GTGTCTATTA AGGAACTGTG TGGACTTCCT 
CCAATTGCCA GTCTGAAGCA GTGCCTGTTG ACCCTGTCGT CTCGGCTCAT CACCAGTGAC 
AATACCCCTA CGGTCTCTCT TGTGATGAAA GACAACTTTC CTTATCTGGA GCCTCTGGGG 
GCAATTCCAG ATGTGCAGAA AAAAATGTTG GCTGCTTATG ACCTGATGAT TCAAGAGAGC 
CGATTTCTCA TAGAAATGGC AGATACAGTC CAGGAAAAGA TTGTGCAGTG TCAGAAATCA 
GGGATGGAAT TCCATGAGGA ACTTCATAAT CTAGGGGCAA AAGAAGGCTT GAAAGGAAGA 
AAACTCAACA AGGCAACTGA GAGCTTTGCT TGGAACATTA CAGTACTGAA GGGCCAAGGA 
GATCTGTTGA AGAACGCCAA GAATGAAGCT GTAGAAAACA TGAAGCAGAT CCAGCTGGCA 
TGCTTGTCTT GTGGACTTAG TAAAGCACCC AGCAGTGGTG CAGAAGCCAA GAGCAAACGC 
AGCCTGGAAG CCATAGAGGA GAAGGAGAGT GGCGAGGAGA ACGGGAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_014110960.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966435.1 Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe45183
Clone ID Related Accession (Same CDS sequence)	XM_014110960.1
Accession Version	XM_014110960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive phospholipase C-like protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGAG AATGCTTCCC CACCCCTCAA CCACCGGACA AGATTTCTCC TCGTCTGCTT 
GGCGAGAAAC TTGATCCTTC AAATCAAAAA TGCGGTGGAA GAAAGAAAAC AGTGTCTTTC 
AGCAGCATGC CCTCGGAAAA GAAAATTAGC AGTGCGAGTG ACTGCATCAG CTTCATGCAA 
GCTGGCTGTG AACTGAAGAA GGTCCGGCCA AATTCTCGTA TTTATAACCG TTTTTTCACT 
CTGGACACAG ACCTCCAAGC TCTTCGCTGG GAACCTTCCA AGAAAGACCT TGAGAAAGCT 
AAGCTTGACA TTTCCGCTAT AAAAGAGATC AGACTGGGGA AGAACACAGA AACATTTAGA 
AACAATGGCC TCGCTGACCA GATTTGTGAG GACTGTGCCT TTTCCATACT CCATGGGGAA 
AACTATGAGT CTCTGGACCT AGTTGCCAAT TCAGCAGACG TAGCAAACAT CTGGGTGTCA 
GGTTTACGGT ACCTGGTTTC TCGAAGTAAG CAACCCCTAG ACTTTATGGA GGGCAATCAG 
AACACACCAA GATTCATGTG GTTGAAAACG GTGTTTGAAG CAGCAGATAT CGATGGGAAT 
GGGATTATGT TGGAAGACAC CTCTGTAGAG TTAATAAAAC AGCTCAACCC TACCCTGAAG 
GAATCCAAGA TCAGGTTAAA GTTTAAAGAA ATCCAGAAGA GCAAAGAAAA ACTAACCACA 
CGAGTGACAG AAGAGGAATT TTGTGAAGCT TTTTGTGAAC TCTGCACCAG GCCAGAAGTA 
TATTTCTTAC TGGTACAGAT ATCTAAAAAC AAAGAATATT TGGATGCCAA CGATCTCATG 
CTCTTTTTAG AAGCTGAGCA AGGAGTCGCC CATATTACTG AGGATATGTG CTTAGACATT 
ATTCGGAGAT ACGAACTTTC CGAAGAGGGA CGTCAAAAAG GGTTTCTTGC AATTGATGGC 
TTTACCCAGT ATCTGTTGTC ACCAGAATGT GACATTTTTG ATCCTGAGCA GAAAAAGGTT 
GCTCAAGATA TGACCCAGCC ATTATCTCAC TACTACATCA ATGCATCTCA CAACACCTAT 
CTCATAGAAG ACCAGTTCAG GGGCCCGGCT GATATTAATG GATATGTTAG AGCTTTGAAA 
ATGGGCTGTC GTAGCATTGA ACTTGATGTC AGTGATGGTT CAGACAATGA ACCGATCCTT 
TGTAATCGAA ATAATGTGAC AACACACCTT TCCTTTCGAA GTGTCATAGA GGTGATAAAT 
AAATTTGCCT TTGTTGCTTC TGAGTACCCA CTCATTCTTT GTTTGGGAAA TCACTGCTCC 
CTACCACAGC AGAAGATAAT GGTTCAACAG ATGAAAAAGG TCTTTGGCGA TAAGCTCTAT 
GTAGAGGCGC CTTTGCCATC AGAAACCTAC CTCCCATCAC CAGAAAAATT GAAAAGAATG 
ATTATTGTGA AAGGAAAGAA ATTGCCTTCT GACCCGGATG TTTTAGAAGG AGAAGTAACA 
GATGAAGATG AAGAAGCGGA AATGTCTCGA AGGCTGTCGG TAGATTACAA TGGTGAGCAG 
AAACAAATCT TGCTGTGTAA GGAGCTCTCT GATTTGGTAT CTATCTGCAA GTCTGTTCAG 
TACAGGGATT TTGAACTGTC TATGAAGAGC CAAAACTATT GGGAAATGTG TTCATTTAGT 
GAAACAGAGG CCAGCCGAAT TGCAAATGAA TACCCAGAGG ATTTTGTAAA TTATAATAAG 
AGGTTCTTAT CAAGGGTCTA TCCAAGTGCC ATGAGGATCG ATTCCAGTAA CTTGAATCCA 
CAGGACTTTT GGAATTGTGG CTGTCAGATT GTGGCAATGA ATTTTCAGAC TCCAGGTCCA 
ATGATGGATC TTCACACAGG CTGGTTTCTT CAAAATGGAG GATGTGGTTA TGTTCTACGG 
CCATCCATCA TGCGAGATGA AGTTTCTTAC TTCAGTGCAA ACACAAAGGG CATTGTACCT 
GGGGTGTCTC CTCTAGCTCT TCATATCAAG ATCATCAGTG GTCAGAATTT TCCAAAGCCC 
AAGGGAGCTT GTGCCAAAGG AGATGTCATA GATCCCTATG TTTGTATAGA GATACATGGA 
ATTCCAGCAG ATTGTTCAGA ACAAAGAACT AAAACTGTCC AGCAGAACAG TGATAACCCT 
ATTTTTGATG AAACTTTTGA GTTTCAAGTA AACCTACCTG AGCTGGCCAT GATTCGTTTT 
GTTGTTCTGG ATGATGACTA CATAGGGGAT GAGTTTATAG GACAATATAC AATACCATTT 
GAATGTTTGC AGCCTGGATA TCGGCATGTG CCCCTGCGCT CTTTTGTGGG TGACATCATG 
GAACATGTAA CCCTTTTTGT CCATATAGCA ATAACTAATC GAAGTGGAGG AGGAAAGGCA 
CAGAAGCGCA GCCTGTCAGT GAGAATGGGG AAGAAAGTTC GAGAATATAC CATGCTCAGG 
AATATCGGCC TTAAGACCAT AGATGACATC TTTAAAATAG CAGTTCATCC CTTACGAGAA 
GCCATAGATA TGAGAGAAAA TATGCAGAAT GCCATAGTGT CTATTAAGGA ACTGTGTGGA 
CTTCCTCCAA TTGCCAGTCT GAAGCAGTGC CTGTTGACCC TGTCGTCTCG GCTCATCACC 
AGTGACAATA CCCCTACGGT CTCTCTTGTG ATGAAAGACA ACTTTCCTTA TCTGGAGCCT 
CTGGGGGCAA TTCCAGATGT GCAGAAAAAA ATGTTGGCTG CTTATGACCT GATGATTCAA 
GAGAGCCGAT TTCTCATAGA AATGGCAGAT ACAGTCCAGG AAAAGATTGT GCAGTGTCAG 
AAATCAGGGA TGGAATTCCA TGAGGAACTT CATAATCTAG GGGCAAAAGA AGGCTTGAAA 
GGAAGAAAAC TCAACAAGGC AACTGAGAGC TTTGCTTGGA ACATTACAGT ACTGAAGGGC 
CAAGGAGATC TGTTGAAGAA CGCCAAGAAT GAAGCTGTAG AAAACATGAA GCAGATCCAG 
CTGGCATGCT TGTCTTGTGG ACTTAGTAAA GCACCCAGCA GTGGTGCAGA AGCCAAGAGC 
AAACGCAGCC TGGAAGCCAT AGAGGAGAAG GAGAGTGGCG AGGAGAACGG GAAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966435.1
CDS	101..3220
Translation	MRGECFPTPQPPDKISPRLLGEKLDPSNQKCGGRKKTVSFSSMPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVAHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVSDGSDNEPILCNRNNVTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKIMVQQMKKVFGDKLYVEAPLPSETYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRLSVDYNGEQKQILLCKELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKRFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPTVSLVMKDNFPYLEPLGAIPDVQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKSGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAVENMKQIQLACLSCGLSKAPSSGAEAKSKRSLEAIEEKESGEENGKL

Target ORF information:

RefSeq Version	XM_014110960.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014110960.1

ORF Insert Sequence:

ATGCGGGGAG AATGCTTCCC CACCCCTCAA CCACCGGACA AGATTTCTCC TCGTCTGCTT 
GGCGAGAAAC TTGATCCTTC AAATCAAAAA TGCGGTGGAA GAAAGAAAAC AGTGTCTTTC 
AGCAGCATGC CCTCGGAAAA GAAAATTAGC AGTGCGAGTG ACTGCATCAG CTTCATGCAA 
GCTGGCTGTG AACTGAAGAA GGTCCGGCCA AATTCTCGTA TTTATAACCG TTTTTTCACT 
CTGGACACAG ACCTCCAAGC TCTTCGCTGG GAACCTTCCA AGAAAGACCT TGAGAAAGCT 
AAGCTTGACA TTTCCGCTAT AAAAGAGATC AGACTGGGGA AGAACACAGA AACATTTAGA 
AACAATGGCC TCGCTGACCA GATTTGTGAG GACTGTGCCT TTTCCATACT CCATGGGGAA 
AACTATGAGT CTCTGGACCT AGTTGCCAAT TCAGCAGACG TAGCAAACAT CTGGGTGTCA 
GGTTTACGGT ACCTGGTTTC TCGAAGTAAG CAACCCCTAG ACTTTATGGA GGGCAATCAG 
AACACACCAA GATTCATGTG GTTGAAAACG GTGTTTGAAG CAGCAGATAT CGATGGGAAT 
GGGATTATGT TGGAAGACAC CTCTGTAGAG TTAATAAAAC AGCTCAACCC TACCCTGAAG 
GAATCCAAGA TCAGGTTAAA GTTTAAAGAA ATCCAGAAGA GCAAAGAAAA ACTAACCACA 
CGAGTGACAG AAGAGGAATT TTGTGAAGCT TTTTGTGAAC TCTGCACCAG GCCAGAAGTA 
TATTTCTTAC TGGTACAGAT ATCTAAAAAC AAAGAATATT TGGATGCCAA CGATCTCATG 
CTCTTTTTAG AAGCTGAGCA AGGAGTCGCC CATATTACTG AGGATATGTG CTTAGACATT 
ATTCGGAGAT ACGAACTTTC CGAAGAGGGA CGTCAAAAAG GGTTTCTTGC AATTGATGGC 
TTTACCCAGT ATCTGTTGTC ACCAGAATGT GACATTTTTG ATCCTGAGCA GAAAAAGGTT 
GCTCAAGATA TGACCCAGCC ATTATCTCAC TACTACATCA ATGCATCTCA CAACACCTAT 
CTCATAGAAG ACCAGTTCAG GGGCCCGGCT GATATTAATG GATATGTTAG AGCTTTGAAA 
ATGGGCTGTC GTAGCATTGA ACTTGATGTC AGTGATGGTT CAGACAATGA ACCGATCCTT 
TGTAATCGAA ATAATGTGAC AACACACCTT TCCTTTCGAA GTGTCATAGA GGTGATAAAT 
AAATTTGCCT TTGTTGCTTC TGAGTACCCA CTCATTCTTT GTTTGGGAAA TCACTGCTCC 
CTACCACAGC AGAAGATAAT GGTTCAACAG ATGAAAAAGG TCTTTGGCGA TAAGCTCTAT 
GTAGAGGCGC CTTTGCCATC AGAAACCTAC CTCCCATCAC CAGAAAAATT GAAAAGAATG 
ATTATTGTGA AAGGAAAGAA ATTGCCTTCT GACCCGGATG TTTTAGAAGG AGAAGTAACA 
GATGAAGATG AAGAAGCGGA AATGTCTCGA AGGCTGTCGG TAGATTACAA TGGTGAGCAG 
AAACAAATCT TGCTGTGTAA GGAGCTCTCT GATTTGGTAT CTATCTGCAA GTCTGTTCAG 
TACAGGGATT TTGAACTGTC TATGAAGAGC CAAAACTATT GGGAAATGTG TTCATTTAGT 
GAAACAGAGG CCAGCCGAAT TGCAAATGAA TACCCAGAGG ATTTTGTAAA TTATAATAAG 
AGGTTCTTAT CAAGGGTCTA TCCAAGTGCC ATGAGGATCG ATTCCAGTAA CTTGAATCCA 
CAGGACTTTT GGAATTGTGG CTGTCAGATT GTGGCAATGA ATTTTCAGAC TCCAGGTCCA 
ATGATGGATC TTCACACAGG CTGGTTTCTT CAAAATGGAG GATGTGGTTA TGTTCTACGG 
CCATCCATCA TGCGAGATGA AGTTTCTTAC TTCAGTGCAA ACACAAAGGG CATTGTACCT 
GGGGTGTCTC CTCTAGCTCT TCATATCAAG ATCATCAGTG GTCAGAATTT TCCAAAGCCC 
AAGGGAGCTT GTGCCAAAGG AGATGTCATA GATCCCTATG TTTGTATAGA GATACATGGA 
ATTCCAGCAG ATTGTTCAGA ACAAAGAACT AAAACTGTCC AGCAGAACAG TGATAACCCT 
ATTTTTGATG AAACTTTTGA GTTTCAAGTA AACCTACCTG AGCTGGCCAT GATTCGTTTT 
GTTGTTCTGG ATGATGACTA CATAGGGGAT GAGTTTATAG GACAATATAC AATACCATTT 
GAATGTTTGC AGCCTGGATA TCGGCATGTG CCCCTGCGCT CTTTTGTGGG TGACATCATG 
GAACATGTAA CCCTTTTTGT CCATATAGCA ATAACTAATC GAAGTGGAGG AGGAAAGGCA 
CAGAAGCGCA GCCTGTCAGT GAGAATGGGG AAGAAAGTTC GAGAATATAC CATGCTCAGG 
AATATCGGCC TTAAGACCAT AGATGACATC TTTAAAATAG CAGTTCATCC CTTACGAGAA 
GCCATAGATA TGAGAGAAAA TATGCAGAAT GCCATAGTGT CTATTAAGGA ACTGTGTGGA 
CTTCCTCCAA TTGCCAGTCT GAAGCAGTGC CTGTTGACCC TGTCGTCTCG GCTCATCACC 
AGTGACAATA CCCCTACGGT CTCTCTTGTG ATGAAAGACA ACTTTCCTTA TCTGGAGCCT 
CTGGGGGCAA TTCCAGATGT GCAGAAAAAA ATGTTGGCTG CTTATGACCT GATGATTCAA 
GAGAGCCGAT TTCTCATAGA AATGGCAGAT ACAGTCCAGG AAAAGATTGT GCAGTGTCAG 
AAATCAGGGA TGGAATTCCA TGAGGAACTT CATAATCTAG GGGCAAAAGA AGGCTTGAAA 
GGAAGAAAAC TCAACAAGGC AACTGAGAGC TTTGCTTGGA ACATTACAGT ACTGAAGGGC 
CAAGGAGATC TGTTGAAGAA CGCCAAGAAT GAAGCTGTAG AAAACATGAA GCAGATCCAG 
CTGGCATGCT TGTCTTGTGG ACTTAGTAAA GCACCCAGCA GTGGTGCAGA AGCCAAGAGC 
AAACGCAGCC TGGAAGCCAT AGAGGAGAAG GAGAGTGGCG AGGAGAACGG GAAGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_022415139.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022270847.1 Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe284877
Clone ID Related Accession (Same CDS sequence)	XM_022415139.1
Accession Version	XM_022415139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive phospholipase C-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGG GCGCGGCGGG CAGGGAGGGT CCCGCGCCGC CCGACGCGGC CGGGGGCGAA 
GACGACCCCC GAGGGGGCCC GGACGCCGCC TGCGGGGAAG GCGTGGCTGC GGCCGCCGGG 
GGCCGCTGGA GGGACCGCCG CAGCGGGGTC GCCCTGCCCG GCGCTGCGGG GGCCGCGGCT 
GACAGCGAGG CGGGCCTCCT GGAGGCGGCG CGCGCGACCC CCCGGCGCAG CAGCATCATC 
AAGGATCCTT CAAATCAAAA ATGCGGTGGA AGAAAGAAAA CAGTGTCTTT CAGCAGCATG 
CCCTCGGAAA AGAAAATTAG CAGTGCGAGT GACTGCATCA GCTTCATGCA AGCTGGCTGT 
GAACTGAAGA AGGTCCGGCC AAATTCTCGT ATTTATAACC GTTTTTTCAC TCTGGACACA 
GACCTCCAAG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TTGAGAAAGC TAAGCTTGAC 
ATTTCCGCTA TAAAAGAGAT CAGACTGGGG AAGAACACAG AAACATTTAG AAACAATGGC 
CTCGCTGACC AGATTTGTGA GGACTGTGCC TTTTCCATAC TCCATGGGGA AAACTATGAG 
TCTCTGGACC TAGTTGCCAA TTCAGCAGAC GTAGCAAACA TCTGGGTGTC AGGTTTACGG 
TACCTGGTTT CTCGAAGTAA GCAACCCCTA GACTTTATGG AGGGCAATCA GAACACACCA 
AGATTCATGT GGTTGAAAAC GGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAGCTCAACC CTACCCTGAA GGAATCCAAG 
ATCAGGTTAA AGTTTAAAGA AATCCAGAAG AGCAAAGAAA AACTAACCAC ACGAGTGACA 
GAAGAGGAAT TTTGTGAAGC TTTTTGTGAA CTCTGCACCA GGCCAGAAGT ATATTTCTTA 
CTGGTACAGA TATCTAAAAA CAAAGAATAT TTGGATGCCA ACGATCTCAT GCTCTTTTTA 
GAAGCTGAGC AAGGAGTCGC CCATATTACT GAGGATATGT GCTTAGACAT TATTCGGAGA 
TACGAACTTT CCGAAGAGGG ACGTCAAAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATCTGTTGT CACCAGAATG TGACATTTTT GATCCTGAGC AGAAAAAGGT TGCTCAAGAT 
ATGACCCAGC CATTATCTCA CTACTACATC AATGCATCTC ACAACACCTA TCTCATAGAA 
GACCAGTTCA GGGGCCCGGC TGATATTAAT GGATATGTTA GAGCTTTGAA AATGGGCTGT 
CGTAGCATTG AACTTGATGT CAGTGATGGT TCAGACAATG AACCGATCCT TTGTAATCGA 
AATAATGTGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTTGCTT CTGAGTACCC ACTCATTCTT TGTTTGGGAA ATCACTGCTC CCTACCACAG 
CAGAAGATAA TGGTTCAACA GATGAAAAAG GTCTTTGGCG ATAAGCTCTA TGTAGAGGCG 
CCTTTGCCAT CAGAAACCTA CCTCCCATCA CCAGAAAAAT TGAAAAGAAT GATTATTGTG 
AAAGGAAAGA AATTGCCTTC TGACCCGGAT GTTTTAGAAG GAGAAGTAAC AGATGAAGAT 
GAAGAAGCGG AAATGTCTCG AAGGCTGTCG GTAGATTACA ATGGTGAGCA GAAACAAATC 
TTGCTGTGTA AGGAGCTCTC TGATTTGGTA TCTATCTGCA AGTCTGTTCA GTACAGGGAT 
TTTGAACTGT CTATGAAGAG CCAAAACTAT TGGGAAATGT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAGGTTCTTA 
TCAAGGGTCT ATCCAAGTGC CATGAGGATC GATTCCAGTA ACTTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAT 
CTTCACACAG GCTGGTTTCT TCAAAATGGA GGATGTGGTT ATGTTCTACG GCCATCCATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGCTC TTCATATCAA GATCATCAGT GGTCAGAATT TTCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GAGATGTCAT AGATCCCTAT GTTTGTATAG AGATACATGG AATTCCAGCA 
GATTGTTCAG AACAAAGAAC TAAAACTGTC CAGCAGAACA GTGATAACCC TATTTTTGAT 
GAAACTTTTG AGTTTCAAGT AAACCTACCT GAGCTGGCCA TGATTCGTTT TGTTGTTCTG 
GATGATGACT ACATAGGGGA TGAGTTTATA GGACAATATA CAATACCATT TGAATGTTTG 
CAGCCTGGAT ATCGGCATGT GCCCCTGCGC TCTTTTGTGG GTGACATCAT GGAACATGTA 
ACCCTTTTTG TCCATATAGC AATAACTAAT CGAAGTGGAG GAGGAAAGGC ACAGAAGCGC 
AGCCTGTCAG TGAGAATGGG GAAGAAAGTT CGAGAATATA CCATGCTCAG GAATATCGGC 
CTTAAGACCA TAGATGACAT CTTTAAAATA GCAGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTATTAAGG AACTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCGTCTC GGCTCATCAC CAGTGACAAT 
ACCCCTACGG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTGGAGCC TCTGGGGGCA 
ATTCCAGATG TGCAGAAAAA AATGTTGGCT GCTTATGACC TGATGATTCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA TACAGTCCAG GAAAAGATTG TGCAGTGTCA GAAATCAGGG 
ATGGAATTCC ATGAGGAACT TCATAATCTA GGGGCAAAAG AAGGCTTGAA AGGAAGAAAA 
CTCAACAAGG CAACTGAGAG CTTTGCTTGG AACATTACAG TACTGAAGAG AAGCCGTGTC 
TTGGAGCCCG CTCCATGGGC CAGACAGCAG ACCACCCTTC ATTGTGAAAC AAAGGGCTTT 
CACACATTGA AACTGTGCAT TTTAGCAGTT GCCAGGTTAG GAGGATTTGC TTTTCCTTTT 
TTTATTTTTA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022270847.1
CDS	22..3276
Translation	MAQGAAGREGPAPPDAAGGEDDPRGGPDAACGEGVAAAAGGRWRDRRSGVALPGAAGAAADSEAGLLEAARATPRRSSIIKDPSNQKCGGRKKTVSFSSMPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVAHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVSDGSDNEPILCNRNNVTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKIMVQQMKKVFGDKLYVEAPLPSETYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRLSVDYNGEQKQILLCKELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKRFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPTVSLVMKDNFPYLEPLGAIPDVQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKSGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKRSRVLEPAPWARQQTTLHCETKGFHTLKLCILAVARLGGFAFPFFIFS

Target ORF information:

RefSeq Version	XM_022415139.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415139.1

ORF Insert Sequence:

ATGGCCCAGG GCGCGGCGGG CAGGGAGGGT CCCGCGCCGC CCGACGCGGC CGGGGGCGAA 
GACGACCCCC GAGGGGGCCC GGACGCCGCC TGCGGGGAAG GCGTGGCTGC GGCCGCCGGG 
GGCCGCTGGA GGGACCGCCG CAGCGGGGTC GCCCTGCCCG GCGCTGCGGG GGCCGCGGCT 
GACAGCGAGG CGGGCCTCCT GGAGGCGGCG CGCGCGACCC CCCGGCGCAG CAGCATCATC 
AAGGATCCTT CAAATCAAAA ATGCGGTGGA AGAAAGAAAA CAGTGTCTTT CAGCAGCATG 
CCCTCGGAAA AGAAAATTAG CAGTGCGAGT GACTGCATCA GCTTCATGCA AGCTGGCTGT 
GAACTGAAGA AGGTCCGGCC AAATTCTCGT ATTTATAACC GTTTTTTCAC TCTGGACACA 
GACCTCCAAG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TTGAGAAAGC TAAGCTTGAC 
ATTTCCGCTA TAAAAGAGAT CAGACTGGGG AAGAACACAG AAACATTTAG AAACAATGGC 
CTCGCTGACC AGATTTGTGA GGACTGTGCC TTTTCCATAC TCCATGGGGA AAACTATGAG 
TCTCTGGACC TAGTTGCCAA TTCAGCAGAC GTAGCAAACA TCTGGGTGTC AGGTTTACGG 
TACCTGGTTT CTCGAAGTAA GCAACCCCTA GACTTTATGG AGGGCAATCA GAACACACCA 
AGATTCATGT GGTTGAAAAC GGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAGCTCAACC CTACCCTGAA GGAATCCAAG 
ATCAGGTTAA AGTTTAAAGA AATCCAGAAG AGCAAAGAAA AACTAACCAC ACGAGTGACA 
GAAGAGGAAT TTTGTGAAGC TTTTTGTGAA CTCTGCACCA GGCCAGAAGT ATATTTCTTA 
CTGGTACAGA TATCTAAAAA CAAAGAATAT TTGGATGCCA ACGATCTCAT GCTCTTTTTA 
GAAGCTGAGC AAGGAGTCGC CCATATTACT GAGGATATGT GCTTAGACAT TATTCGGAGA 
TACGAACTTT CCGAAGAGGG ACGTCAAAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATCTGTTGT CACCAGAATG TGACATTTTT GATCCTGAGC AGAAAAAGGT TGCTCAAGAT 
ATGACCCAGC CATTATCTCA CTACTACATC AATGCATCTC ACAACACCTA TCTCATAGAA 
GACCAGTTCA GGGGCCCGGC TGATATTAAT GGATATGTTA GAGCTTTGAA AATGGGCTGT 
CGTAGCATTG AACTTGATGT CAGTGATGGT TCAGACAATG AACCGATCCT TTGTAATCGA 
AATAATGTGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTTGCTT CTGAGTACCC ACTCATTCTT TGTTTGGGAA ATCACTGCTC CCTACCACAG 
CAGAAGATAA TGGTTCAACA GATGAAAAAG GTCTTTGGCG ATAAGCTCTA TGTAGAGGCG 
CCTTTGCCAT CAGAAACCTA CCTCCCATCA CCAGAAAAAT TGAAAAGAAT GATTATTGTG 
AAAGGAAAGA AATTGCCTTC TGACCCGGAT GTTTTAGAAG GAGAAGTAAC AGATGAAGAT 
GAAGAAGCGG AAATGTCTCG AAGGCTGTCG GTAGATTACA ATGGTGAGCA GAAACAAATC 
TTGCTGTGTA AGGAGCTCTC TGATTTGGTA TCTATCTGCA AGTCTGTTCA GTACAGGGAT 
TTTGAACTGT CTATGAAGAG CCAAAACTAT TGGGAAATGT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAGGTTCTTA 
TCAAGGGTCT ATCCAAGTGC CATGAGGATC GATTCCAGTA ACTTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAT 
CTTCACACAG GCTGGTTTCT TCAAAATGGA GGATGTGGTT ATGTTCTACG GCCATCCATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGCTC TTCATATCAA GATCATCAGT GGTCAGAATT TTCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GAGATGTCAT AGATCCCTAT GTTTGTATAG AGATACATGG AATTCCAGCA 
GATTGTTCAG AACAAAGAAC TAAAACTGTC CAGCAGAACA GTGATAACCC TATTTTTGAT 
GAAACTTTTG AGTTTCAAGT AAACCTACCT GAGCTGGCCA TGATTCGTTT TGTTGTTCTG 
GATGATGACT ACATAGGGGA TGAGTTTATA GGACAATATA CAATACCATT TGAATGTTTG 
CAGCCTGGAT ATCGGCATGT GCCCCTGCGC TCTTTTGTGG GTGACATCAT GGAACATGTA 
ACCCTTTTTG TCCATATAGC AATAACTAAT CGAAGTGGAG GAGGAAAGGC ACAGAAGCGC 
AGCCTGTCAG TGAGAATGGG GAAGAAAGTT CGAGAATATA CCATGCTCAG GAATATCGGC 
CTTAAGACCA TAGATGACAT CTTTAAAATA GCAGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTATTAAGG AACTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCGTCTC GGCTCATCAC CAGTGACAAT 
ACCCCTACGG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTGGAGCC TCTGGGGGCA 
ATTCCAGATG TGCAGAAAAA AATGTTGGCT GCTTATGACC TGATGATTCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA TACAGTCCAG GAAAAGATTG TGCAGTGTCA GAAATCAGGG 
ATGGAATTCC ATGAGGAACT TCATAATCTA GGGGCAAAAG AAGGCTTGAA AGGAAGAAAA 
CTCAACAAGG CAACTGAGAG CTTTGCTTGG AACATTACAG TACTGAAGAG AAGCCGTGTC 
TTGGAGCCCG CTCCATGGGC CAGACAGCAG ACCACCCTTC ATTGTGAAAC AAAGGGCTTT 
CACACATTGA AACTGTGCAT TTTAGCAGTT GCCAGGTTAG GAGGATTTGC TTTTCCTTTT 
TTTATTTTTA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_536020.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536020.4 Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X1, mRNA.

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CloneID	OCe31326
Clone ID Related Accession (Same CDS sequence)	XM_536020.6 , XM_536020.5
Accession Version	XM_536020.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	inactive phospholipase C-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_536020.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGG GCGCGGCGGG CAGGGAGGGT CCCGCGCCGC CCGACGCGGC CGGGGGCGAA 
GACGACCCCC GAGGGGGCCC GGACGCCGCC TGCGGGGAAG GCGTGGCTGC GGCCGCCGGG 
GGCCGCTGGA GGGACCGCCG CAGCGGGGTC GCCCTGCCCG GCGCTGCGGG GGCCGCGGCT 
GACAGCGAGG CGGGCCTCCT GGAGGCGGCG CGCGCGACCC CCCGGCGCAG CAGCATCATC 
AAGGATCCTT CAAATCAAAA ATGCGGTGGA AGAAAGAAAA CAGTGTCTTT CAGCAGCATG 
CCCTCGGAAA AGAAAATTAG CAGTGCGAGT GACTGCATCA GCTTCATGCA AGCTGGCTGT 
GAACTGAAGA AGGTCCGGCC AAATTCTCGT ATTTATAACC GTTTTTTCAC TCTGGACACA 
GACCTCCAAG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TTGAGAAAGC TAAGCTTGAC 
ATTTCCGCTA TAAAAGAGAT CAGACTGGGG AAGAACACAG AAACATTTAG AAACAATGGC 
CTCGCTGACC AGATTTGTGA GGACTGTGCC TTTTCCATAC TCCATGGGGA AAACTATGAG 
TCTCTGGACC TAGTTGCCAA TTCAGCAGAC GTAGCAAACA TCTGGGTGTC AGGTTTACGG 
TACCTGGTTT CTCGAAGTAA GCAACCCCTA GACTTTATGG AGGGCAATCA GAACACACCA 
AGATTCATGT GGTTGAAAAC GGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAGCTCAACC CTACCCTGAA GGAATCCAAG 
ATCAGGTTAA AGTTTAAAGA AATCCAGAAG AGCAAAGAAA AACTAACCAC ACGAGTGACA 
GAAGAGGAAT TTTGTGAAGC TTTTTGTGAA CTCTGCACCA GGCCAGAAGT ATATTTCTTA 
CTGGTACAGA TATCTAAAAA CAAAGAATAT TTGGATGCCA ACGATCTCAT GCTCTTTTTA 
GAAGCTGAGC AAGGAGTCGC CCATATTACT GAGGATATGT GCTTAGACAT TATTCGGAGA 
TACGAACTTT CCGAAGAGGG ACGTCAAAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATCTGTTGT CACCAGAATG TGACATTTTT GATCCTGAGC AGAAAAAGGT TGCTCAAGAT 
ATGACCCAGC CATTATCTCA CTACTACATC AATGCATCTC ACAACACCTA TCTCATAGAA 
GACCAGTTCA GGGGCCCGGC TGATATTAAT GGATATGTTA GAGCTTTGAA AATGGGCTGT 
CGTAGCATTG AACTTGATGT CAGTGATGGT TCAGACAATG AACCGATCCT TTGTAATCGA 
AATAATGTGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTTGCTT CTGAGTACCC ACTCATTCTT TGTTTGGGAA ATCACTGCTC CCTACCACAG 
CAGAAGATAA TGGTTCAACA GATGAAAAAG GTCTTTGGCG ATAAGCTCTA TGTAGAGGCG 
CCTTTGCCAT CAGAAACCTA CCTCCCATCA CCAGAAAAAT TGAAAAGAAT GATTATTGTG 
AAAGGAAAGA AATTGCCTTC TGACCCGGAT GTTTTAGAAG GAGAAGTAAC AGATGAAGAT 
GAAGAAGCGG AAATGTCTCG AAGGCTGTCG GTAGATTACA ATGGTGAGCA GAAACAAATC 
TTGCTGTGTA AGGAGCTCTC TGATTTGGTA TCTATCTGCA AGTCTGTTCA GTACAGGGAT 
TTTGAACTGT CTATGAAGAG CCAAAACTAT TGGGAAATGT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAGGTTCTTA 
TCAAGGGTCT ATCCAAGTGC CATGAGGATC GATTCCAGTA ACTTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAT 
CTTCACACAG GCTGGTTTCT TCAAAATGGA GGATGTGGTT ATGTTCTACG GCCATCCATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGCTC TTCATATCAA GATCATCAGT GGTCAGAATT TTCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GAGATGTCAT AGATCCCTAT GTTTGTATAG AGATACATGG AATTCCAGCA 
GATTGTTCAG AACAAAGAAC TAAAACTGTC CAGCAGAACA GTGATAACCC TATTTTTGAT 
GAAACTTTTG AGTTTCAAGT AAACCTACCT GAGCTGGCCA TGATTCGTTT TGTTGTTCTG 
GATGATGACT ACATAGGGGA TGAGTTTATA GGACAATATA CAATACCATT TGAATGTTTG 
CAGCCTGGAT ATCGGCATGT GCCCCTGCGC TCTTTTGTGG GTGACATCAT GGAACATGTA 
ACCCTTTTTG TCCATATAGC AATAACTAAT CGAAGTGGAG GAGGAAAGGC ACAGAAGCGC 
AGCCTGTCAG TGAGAATGGG GAAGAAAGTT CGAGAATATA CCATGCTCAG GAATATCGGC 
CTTAAGACCA TAGATGACAT CTTTAAAATA GCAGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTATTAAGG AACTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCGTCTC GGCTCATCAC CAGTGACAAT 
ACCCCTACGG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTGGAGCC TCTGGGGGCA 
ATTCCAGATG TGCAGAAAAA AATGTTGGCT GCTTATGACC TGATGATTCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA TACAGTCCAG GAAAAGATTG TGCAGTGTCA GAAATCAGGG 
ATGGAATTCC ATGAGGAACT TCATAATCTA GGGGCAAAAG AAGGCTTGAA AGGAAGAAAA 
CTCAACAAGG CAACTGAGAG CTTTGCTTGG AACATTACAG TACTGAAGGG CCAAGGAGAT 
CTGTTGAAGA ACGCCAAGAA TGAAGCTGTA GAAAACATGA AGCAGATCCA GCTGGCATGC 
TTGTCTTGTG GACTTAGTAA AGCACCCAGC AGTGGTGCAG AAGCCAAGAG CAAACGCAGC 
CTGGAAGCCA TAGAGGAGAA GGAGAGTGGC GAGGAGAACG GGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536020.4
CDS	16..3306
Translation	MAQGAAGREGPAPPDAAGGEDDPRGGPDAACGEGVAAAAGGRWRDRRSGVALPGAAGAAADSEAGLLEAARATPRRSSIIKDPSNQKCGGRKKTVSFSSMPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVAHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVSDGSDNEPILCNRNNVTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKIMVQQMKKVFGDKLYVEAPLPSETYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRLSVDYNGEQKQILLCKELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKRFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPTVSLVMKDNFPYLEPLGAIPDVQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKSGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAVENMKQIQLACLSCGLSKAPSSGAEAKSKRSLEAIEEKESGEENGKL

Target ORF information:

RefSeq Version	XM_536020.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris phospholipase C like 1 (inactive) (PLCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536020.6

ORF Insert Sequence:

ATGGCCCAGG GCGCGGCGGG CAGGGAGGGT CCCGCGCCGC CCGACGCGGC CGGGGGCGAA 
GACGACCCCC GAGGGGGCCC GGACGCCGCC TGCGGGGAAG GCGTGGCTGC GGCCGCCGGG 
GGCCGCTGGA GGGACCGCCG CAGCGGGGTC GCCCTGCCCG GCGCTGCGGG GGCCGCGGCT 
GACAGCGAGG CGGGCCTCCT GGAGGCGGCG CGCGCGACCC CCCGGCGCAG CAGCATCATC 
AAGGATCCTT CAAATCAAAA ATGCGGTGGA AGAAAGAAAA CAGTGTCTTT CAGCAGCATG 
CCCTCGGAAA AGAAAATTAG CAGTGCGAGT GACTGCATCA GCTTCATGCA AGCTGGCTGT 
GAACTGAAGA AGGTCCGGCC AAATTCTCGT ATTTATAACC GTTTTTTCAC TCTGGACACA 
GACCTCCAAG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TTGAGAAAGC TAAGCTTGAC 
ATTTCCGCTA TAAAAGAGAT CAGACTGGGG AAGAACACAG AAACATTTAG AAACAATGGC 
CTCGCTGACC AGATTTGTGA GGACTGTGCC TTTTCCATAC TCCATGGGGA AAACTATGAG 
TCTCTGGACC TAGTTGCCAA TTCAGCAGAC GTAGCAAACA TCTGGGTGTC AGGTTTACGG 
TACCTGGTTT CTCGAAGTAA GCAACCCCTA GACTTTATGG AGGGCAATCA GAACACACCA 
AGATTCATGT GGTTGAAAAC GGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAGCTCAACC CTACCCTGAA GGAATCCAAG 
ATCAGGTTAA AGTTTAAAGA AATCCAGAAG AGCAAAGAAA AACTAACCAC ACGAGTGACA 
GAAGAGGAAT TTTGTGAAGC TTTTTGTGAA CTCTGCACCA GGCCAGAAGT ATATTTCTTA 
CTGGTACAGA TATCTAAAAA CAAAGAATAT TTGGATGCCA ACGATCTCAT GCTCTTTTTA 
GAAGCTGAGC AAGGAGTCGC CCATATTACT GAGGATATGT GCTTAGACAT TATTCGGAGA 
TACGAACTTT CCGAAGAGGG ACGTCAAAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATCTGTTGT CACCAGAATG TGACATTTTT GATCCTGAGC AGAAAAAGGT TGCTCAAGAT 
ATGACCCAGC CATTATCTCA CTACTACATC AATGCATCTC ACAACACCTA TCTCATAGAA 
GACCAGTTCA GGGGCCCGGC TGATATTAAT GGATATGTTA GAGCTTTGAA AATGGGCTGT 
CGTAGCATTG AACTTGATGT CAGTGATGGT TCAGACAATG AACCGATCCT TTGTAATCGA 
AATAATGTGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTTGCTT CTGAGTACCC ACTCATTCTT TGTTTGGGAA ATCACTGCTC CCTACCACAG 
CAGAAGATAA TGGTTCAACA GATGAAAAAG GTCTTTGGCG ATAAGCTCTA TGTAGAGGCG 
CCTTTGCCAT CAGAAACCTA CCTCCCATCA CCAGAAAAAT TGAAAAGAAT GATTATTGTG 
AAAGGAAAGA AATTGCCTTC TGACCCGGAT GTTTTAGAAG GAGAAGTAAC AGATGAAGAT 
GAAGAAGCGG AAATGTCTCG AAGGCTGTCG GTAGATTACA ATGGTGAGCA GAAACAAATC 
TTGCTGTGTA AGGAGCTCTC TGATTTGGTA TCTATCTGCA AGTCTGTTCA GTACAGGGAT 
TTTGAACTGT CTATGAAGAG CCAAAACTAT TGGGAAATGT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAGGTTCTTA 
TCAAGGGTCT ATCCAAGTGC CATGAGGATC GATTCCAGTA ACTTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAT 
CTTCACACAG GCTGGTTTCT TCAAAATGGA GGATGTGGTT ATGTTCTACG GCCATCCATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGCTC TTCATATCAA GATCATCAGT GGTCAGAATT TTCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GAGATGTCAT AGATCCCTAT GTTTGTATAG AGATACATGG AATTCCAGCA 
GATTGTTCAG AACAAAGAAC TAAAACTGTC CAGCAGAACA GTGATAACCC TATTTTTGAT 
GAAACTTTTG AGTTTCAAGT AAACCTACCT GAGCTGGCCA TGATTCGTTT TGTTGTTCTG 
GATGATGACT ACATAGGGGA TGAGTTTATA GGACAATATA CAATACCATT TGAATGTTTG 
CAGCCTGGAT ATCGGCATGT GCCCCTGCGC TCTTTTGTGG GTGACATCAT GGAACATGTA 
ACCCTTTTTG TCCATATAGC AATAACTAAT CGAAGTGGAG GAGGAAAGGC ACAGAAGCGC 
AGCCTGTCAG TGAGAATGGG GAAGAAAGTT CGAGAATATA CCATGCTCAG GAATATCGGC 
CTTAAGACCA TAGATGACAT CTTTAAAATA GCAGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTATTAAGG AACTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCGTCTC GGCTCATCAC CAGTGACAAT 
ACCCCTACGG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTGGAGCC TCTGGGGGCA 
ATTCCAGATG TGCAGAAAAA AATGTTGGCT GCTTATGACC TGATGATTCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA TACAGTCCAG GAAAAGATTG TGCAGTGTCA GAAATCAGGG 
ATGGAATTCC ATGAGGAACT TCATAATCTA GGGGCAAAAG AAGGCTTGAA AGGAAGAAAA 
CTCAACAAGG CAACTGAGAG CTTTGCTTGG AACATTACAG TACTGAAGGG CCAAGGAGAT 
CTGTTGAAGA ACGCCAAGAA TGAAGCTGTA GAAAACATGA AGCAGATCCA GCTGGCATGC 
TTGTCTTGTG GACTTAGTAA AGCACCCAGC AGTGGTGCAG AAGCCAAGAG CAAACGCAGC 
CTGGAAGCCA TAGAGGAGAA GGAGAGTGGC GAGGAGAACG GGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLCL1 ( XM_536020.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_536020.4 Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X1, mRNA.

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CloneID	OCe31326
Clone ID Related Accession (Same CDS sequence)	XM_536020.6 , XM_536020.5
Accession Version	XM_536020.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	inactive phospholipase C-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_536020.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGG GCGCGGCGGG CAGGGAGGGT CCCGCGCCGC CCGACGCGGC CGGGGGCGAA 
GACGACCCCC GAGGGGGCCC GGACGCCGCC TGCGGGGAAG GCGTGGCTGC GGCCGCCGGG 
GGCCGCTGGA GGGACCGCCG CAGCGGGGTC GCCCTGCCCG GCGCTGCGGG GGCCGCGGCT 
GACAGCGAGG CGGGCCTCCT GGAGGCGGCG CGCGCGACCC CCCGGCGCAG CAGCATCATC 
AAGGATCCTT CAAATCAAAA ATGCGGTGGA AGAAAGAAAA CAGTGTCTTT CAGCAGCATG 
CCCTCGGAAA AGAAAATTAG CAGTGCGAGT GACTGCATCA GCTTCATGCA AGCTGGCTGT 
GAACTGAAGA AGGTCCGGCC AAATTCTCGT ATTTATAACC GTTTTTTCAC TCTGGACACA 
GACCTCCAAG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TTGAGAAAGC TAAGCTTGAC 
ATTTCCGCTA TAAAAGAGAT CAGACTGGGG AAGAACACAG AAACATTTAG AAACAATGGC 
CTCGCTGACC AGATTTGTGA GGACTGTGCC TTTTCCATAC TCCATGGGGA AAACTATGAG 
TCTCTGGACC TAGTTGCCAA TTCAGCAGAC GTAGCAAACA TCTGGGTGTC AGGTTTACGG 
TACCTGGTTT CTCGAAGTAA GCAACCCCTA GACTTTATGG AGGGCAATCA GAACACACCA 
AGATTCATGT GGTTGAAAAC GGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAGCTCAACC CTACCCTGAA GGAATCCAAG 
ATCAGGTTAA AGTTTAAAGA AATCCAGAAG AGCAAAGAAA AACTAACCAC ACGAGTGACA 
GAAGAGGAAT TTTGTGAAGC TTTTTGTGAA CTCTGCACCA GGCCAGAAGT ATATTTCTTA 
CTGGTACAGA TATCTAAAAA CAAAGAATAT TTGGATGCCA ACGATCTCAT GCTCTTTTTA 
GAAGCTGAGC AAGGAGTCGC CCATATTACT GAGGATATGT GCTTAGACAT TATTCGGAGA 
TACGAACTTT CCGAAGAGGG ACGTCAAAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATCTGTTGT CACCAGAATG TGACATTTTT GATCCTGAGC AGAAAAAGGT TGCTCAAGAT 
ATGACCCAGC CATTATCTCA CTACTACATC AATGCATCTC ACAACACCTA TCTCATAGAA 
GACCAGTTCA GGGGCCCGGC TGATATTAAT GGATATGTTA GAGCTTTGAA AATGGGCTGT 
CGTAGCATTG AACTTGATGT CAGTGATGGT TCAGACAATG AACCGATCCT TTGTAATCGA 
AATAATGTGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTTGCTT CTGAGTACCC ACTCATTCTT TGTTTGGGAA ATCACTGCTC CCTACCACAG 
CAGAAGATAA TGGTTCAACA GATGAAAAAG GTCTTTGGCG ATAAGCTCTA TGTAGAGGCG 
CCTTTGCCAT CAGAAACCTA CCTCCCATCA CCAGAAAAAT TGAAAAGAAT GATTATTGTG 
AAAGGAAAGA AATTGCCTTC TGACCCGGAT GTTTTAGAAG GAGAAGTAAC AGATGAAGAT 
GAAGAAGCGG AAATGTCTCG AAGGCTGTCG GTAGATTACA ATGGTGAGCA GAAACAAATC 
TTGCTGTGTA AGGAGCTCTC TGATTTGGTA TCTATCTGCA AGTCTGTTCA GTACAGGGAT 
TTTGAACTGT CTATGAAGAG CCAAAACTAT TGGGAAATGT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAGGTTCTTA 
TCAAGGGTCT ATCCAAGTGC CATGAGGATC GATTCCAGTA ACTTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAT 
CTTCACACAG GCTGGTTTCT TCAAAATGGA GGATGTGGTT ATGTTCTACG GCCATCCATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGCTC TTCATATCAA GATCATCAGT GGTCAGAATT TTCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GAGATGTCAT AGATCCCTAT GTTTGTATAG AGATACATGG AATTCCAGCA 
GATTGTTCAG AACAAAGAAC TAAAACTGTC CAGCAGAACA GTGATAACCC TATTTTTGAT 
GAAACTTTTG AGTTTCAAGT AAACCTACCT GAGCTGGCCA TGATTCGTTT TGTTGTTCTG 
GATGATGACT ACATAGGGGA TGAGTTTATA GGACAATATA CAATACCATT TGAATGTTTG 
CAGCCTGGAT ATCGGCATGT GCCCCTGCGC TCTTTTGTGG GTGACATCAT GGAACATGTA 
ACCCTTTTTG TCCATATAGC AATAACTAAT CGAAGTGGAG GAGGAAAGGC ACAGAAGCGC 
AGCCTGTCAG TGAGAATGGG GAAGAAAGTT CGAGAATATA CCATGCTCAG GAATATCGGC 
CTTAAGACCA TAGATGACAT CTTTAAAATA GCAGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTATTAAGG AACTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCGTCTC GGCTCATCAC CAGTGACAAT 
ACCCCTACGG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTGGAGCC TCTGGGGGCA 
ATTCCAGATG TGCAGAAAAA AATGTTGGCT GCTTATGACC TGATGATTCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA TACAGTCCAG GAAAAGATTG TGCAGTGTCA GAAATCAGGG 
ATGGAATTCC ATGAGGAACT TCATAATCTA GGGGCAAAAG AAGGCTTGAA AGGAAGAAAA 
CTCAACAAGG CAACTGAGAG CTTTGCTTGG AACATTACAG TACTGAAGGG CCAAGGAGAT 
CTGTTGAAGA ACGCCAAGAA TGAAGCTGTA GAAAACATGA AGCAGATCCA GCTGGCATGC 
TTGTCTTGTG GACTTAGTAA AGCACCCAGC AGTGGTGCAG AAGCCAAGAG CAAACGCAGC 
CTGGAAGCCA TAGAGGAGAA GGAGAGTGGC GAGGAGAACG GGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_536020.4
CDS	167..3457
Translation	MAQGAAGREGPAPPDAAGGEDDPRGGPDAACGEGVAAAAGGRWRDRRSGVALPGAAGAAADSEAGLLEAARATPRRSSIIKDPSNQKCGGRKKTVSFSSMPSEKKISSASDCISFMQAGCELKKVRPNSRIYNRFFTLDTDLQALRWEPSKKDLEKAKLDISAIKEIRLGKNTETFRNNGLADQICEDCAFSILHGENYESLDLVANSADVANIWVSGLRYLVSRSKQPLDFMEGNQNTPRFMWLKTVFEAADIDGNGIMLEDTSVELIKQLNPTLKESKIRLKFKEIQKSKEKLTTRVTEEEFCEAFCELCTRPEVYFLLVQISKNKEYLDANDLMLFLEAEQGVAHITEDMCLDIIRRYELSEEGRQKGFLAIDGFTQYLLSPECDIFDPEQKKVAQDMTQPLSHYYINASHNTYLIEDQFRGPADINGYVRALKMGCRSIELDVSDGSDNEPILCNRNNVTTHLSFRSVIEVINKFAFVASEYPLILCLGNHCSLPQQKIMVQQMKKVFGDKLYVEAPLPSETYLPSPEKLKRMIIVKGKKLPSDPDVLEGEVTDEDEEAEMSRRLSVDYNGEQKQILLCKELSDLVSICKSVQYRDFELSMKSQNYWEMCSFSETEASRIANEYPEDFVNYNKRFLSRVYPSAMRIDSSNLNPQDFWNCGCQIVAMNFQTPGPMMDLHTGWFLQNGGCGYVLRPSIMRDEVSYFSANTKGIVPGVSPLALHIKIISGQNFPKPKGACAKGDVIDPYVCIEIHGIPADCSEQRTKTVQQNSDNPIFDETFEFQVNLPELAMIRFVVLDDDYIGDEFIGQYTIPFECLQPGYRHVPLRSFVGDIMEHVTLFVHIAITNRSGGGKAQKRSLSVRMGKKVREYTMLRNIGLKTIDDIFKIAVHPLREAIDMRENMQNAIVSIKELCGLPPIASLKQCLLTLSSRLITSDNTPTVSLVMKDNFPYLEPLGAIPDVQKKMLAAYDLMIQESRFLIEMADTVQEKIVQCQKSGMEFHEELHNLGAKEGLKGRKLNKATESFAWNITVLKGQGDLLKNAKNEAVENMKQIQLACLSCGLSKAPSSGAEAKSKRSLEAIEEKESGEENGKL

Target ORF information:

RefSeq Version	XM_536020.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris phospholipase C-like 1 (PLCL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_536020.5

ORF Insert Sequence:

ATGGCCCAGG GCGCGGCGGG CAGGGAGGGT CCCGCGCCGC CCGACGCGGC CGGGGGCGAA 
GACGACCCCC GAGGGGGCCC GGACGCCGCC TGCGGGGAAG GCGTGGCTGC GGCCGCCGGG 
GGCCGCTGGA GGGACCGCCG CAGCGGGGTC GCCCTGCCCG GCGCTGCGGG GGCCGCGGCT 
GACAGCGAGG CGGGCCTCCT GGAGGCGGCG CGCGCGACCC CCCGGCGCAG CAGCATCATC 
AAGGATCCTT CAAATCAAAA ATGCGGTGGA AGAAAGAAAA CAGTGTCTTT CAGCAGCATG 
CCCTCGGAAA AGAAAATTAG CAGTGCGAGT GACTGCATCA GCTTCATGCA AGCTGGCTGT 
GAACTGAAGA AGGTCCGGCC AAATTCTCGT ATTTATAACC GTTTTTTCAC TCTGGACACA 
GACCTCCAAG CTCTTCGCTG GGAACCTTCC AAGAAAGACC TTGAGAAAGC TAAGCTTGAC 
ATTTCCGCTA TAAAAGAGAT CAGACTGGGG AAGAACACAG AAACATTTAG AAACAATGGC 
CTCGCTGACC AGATTTGTGA GGACTGTGCC TTTTCCATAC TCCATGGGGA AAACTATGAG 
TCTCTGGACC TAGTTGCCAA TTCAGCAGAC GTAGCAAACA TCTGGGTGTC AGGTTTACGG 
TACCTGGTTT CTCGAAGTAA GCAACCCCTA GACTTTATGG AGGGCAATCA GAACACACCA 
AGATTCATGT GGTTGAAAAC GGTGTTTGAA GCAGCAGATA TCGATGGGAA TGGGATTATG 
TTGGAAGACA CCTCTGTAGA GTTAATAAAA CAGCTCAACC CTACCCTGAA GGAATCCAAG 
ATCAGGTTAA AGTTTAAAGA AATCCAGAAG AGCAAAGAAA AACTAACCAC ACGAGTGACA 
GAAGAGGAAT TTTGTGAAGC TTTTTGTGAA CTCTGCACCA GGCCAGAAGT ATATTTCTTA 
CTGGTACAGA TATCTAAAAA CAAAGAATAT TTGGATGCCA ACGATCTCAT GCTCTTTTTA 
GAAGCTGAGC AAGGAGTCGC CCATATTACT GAGGATATGT GCTTAGACAT TATTCGGAGA 
TACGAACTTT CCGAAGAGGG ACGTCAAAAA GGGTTTCTTG CAATTGATGG CTTTACCCAG 
TATCTGTTGT CACCAGAATG TGACATTTTT GATCCTGAGC AGAAAAAGGT TGCTCAAGAT 
ATGACCCAGC CATTATCTCA CTACTACATC AATGCATCTC ACAACACCTA TCTCATAGAA 
GACCAGTTCA GGGGCCCGGC TGATATTAAT GGATATGTTA GAGCTTTGAA AATGGGCTGT 
CGTAGCATTG AACTTGATGT CAGTGATGGT TCAGACAATG AACCGATCCT TTGTAATCGA 
AATAATGTGA CAACACACCT TTCCTTTCGA AGTGTCATAG AGGTGATAAA TAAATTTGCC 
TTTGTTGCTT CTGAGTACCC ACTCATTCTT TGTTTGGGAA ATCACTGCTC CCTACCACAG 
CAGAAGATAA TGGTTCAACA GATGAAAAAG GTCTTTGGCG ATAAGCTCTA TGTAGAGGCG 
CCTTTGCCAT CAGAAACCTA CCTCCCATCA CCAGAAAAAT TGAAAAGAAT GATTATTGTG 
AAAGGAAAGA AATTGCCTTC TGACCCGGAT GTTTTAGAAG GAGAAGTAAC AGATGAAGAT 
GAAGAAGCGG AAATGTCTCG AAGGCTGTCG GTAGATTACA ATGGTGAGCA GAAACAAATC 
TTGCTGTGTA AGGAGCTCTC TGATTTGGTA TCTATCTGCA AGTCTGTTCA GTACAGGGAT 
TTTGAACTGT CTATGAAGAG CCAAAACTAT TGGGAAATGT GTTCATTTAG TGAAACAGAG 
GCCAGCCGAA TTGCAAATGA ATACCCAGAG GATTTTGTAA ATTATAATAA GAGGTTCTTA 
TCAAGGGTCT ATCCAAGTGC CATGAGGATC GATTCCAGTA ACTTGAATCC ACAGGACTTT 
TGGAATTGTG GCTGTCAGAT TGTGGCAATG AATTTTCAGA CTCCAGGTCC AATGATGGAT 
CTTCACACAG GCTGGTTTCT TCAAAATGGA GGATGTGGTT ATGTTCTACG GCCATCCATC 
ATGCGAGATG AAGTTTCTTA CTTCAGTGCA AACACAAAGG GCATTGTACC TGGGGTGTCT 
CCTCTAGCTC TTCATATCAA GATCATCAGT GGTCAGAATT TTCCAAAGCC CAAGGGAGCT 
TGTGCCAAAG GAGATGTCAT AGATCCCTAT GTTTGTATAG AGATACATGG AATTCCAGCA 
GATTGTTCAG AACAAAGAAC TAAAACTGTC CAGCAGAACA GTGATAACCC TATTTTTGAT 
GAAACTTTTG AGTTTCAAGT AAACCTACCT GAGCTGGCCA TGATTCGTTT TGTTGTTCTG 
GATGATGACT ACATAGGGGA TGAGTTTATA GGACAATATA CAATACCATT TGAATGTTTG 
CAGCCTGGAT ATCGGCATGT GCCCCTGCGC TCTTTTGTGG GTGACATCAT GGAACATGTA 
ACCCTTTTTG TCCATATAGC AATAACTAAT CGAAGTGGAG GAGGAAAGGC ACAGAAGCGC 
AGCCTGTCAG TGAGAATGGG GAAGAAAGTT CGAGAATATA CCATGCTCAG GAATATCGGC 
CTTAAGACCA TAGATGACAT CTTTAAAATA GCAGTTCATC CCTTACGAGA AGCCATAGAT 
ATGAGAGAAA ATATGCAGAA TGCCATAGTG TCTATTAAGG AACTGTGTGG ACTTCCTCCA 
ATTGCCAGTC TGAAGCAGTG CCTGTTGACC CTGTCGTCTC GGCTCATCAC CAGTGACAAT 
ACCCCTACGG TCTCTCTTGT GATGAAAGAC AACTTTCCTT ATCTGGAGCC TCTGGGGGCA 
ATTCCAGATG TGCAGAAAAA AATGTTGGCT GCTTATGACC TGATGATTCA AGAGAGCCGA 
TTTCTCATAG AAATGGCAGA TACAGTCCAG GAAAAGATTG TGCAGTGTCA GAAATCAGGG 
ATGGAATTCC ATGAGGAACT TCATAATCTA GGGGCAAAAG AAGGCTTGAA AGGAAGAAAA 
CTCAACAAGG CAACTGAGAG CTTTGCTTGG AACATTACAG TACTGAAGGG CCAAGGAGAT 
CTGTTGAAGA ACGCCAAGAA TGAAGCTGTA GAAAACATGA AGCAGATCCA GCTGGCATGC 
TTGTCTTGTG GACTTAGTAA AGCACCCAGC AGTGGTGCAG AAGCCAAGAG CAAACGCAGC 
CTGGAAGCCA TAGAGGAGAA GGAGAGTGGC GAGGAGAACG GGAAGCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PLCL1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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