GAD1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAD1
Entrez Gene ID	478794
Full Name	glutamate decarboxylase 1
Synonyms	GAD67
General protein information	Preferred Names glutamate decarboxylase 1 Names glutamate decarboxylase 1 67 kDa glutamic acid decarboxylase GAD-67 glutamate decarboxylase 67 kDa isoform
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001097543.1	NP_001091012.1	glutamate decarboxylase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GAD1	NP_000808.2
Rattus norvegicus (Norway rat)	Gad1	NP_058703.1
Canis lupus familiaris (dog)	GAD1	NP_001091012.1
Pan troglodytes (chimpanzee)	GAD1	NP_001029285.1
Xenopus tropicalis (tropical clawed frog)	gad1	XP_004917767.1
Gallus gallus (chicken)	GAD1	NP_990244.1
Drosophila melanogaster (fruit fly)	Gad1	NP_728931.1
Neurospora crassa	NCU06112	XP_959974.1
	GAD1	XP_001082995.1
Bos taurus (cattle)	GAD1	NP_001069224.1
Mus musculus (house mouse)	Gad1	NP_032103.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005866	XP_315894.2
Caenorhabditis elegans (roundworm)	unc-25	NP_499689.1
Magnaporthe oryzae (rice blast fungus)	MGG_03869	XP_003720021.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparison of GAD65 and 67 immunoreactivity in the lumbar spinal cord between young adult and aged dogs.
Lee HJ, Choi JH, Ahn JH, Lee CH, Yoo KY, Hwang IK, Kim JS, Kim C, Lee YL, Shin HC, Won MH
Neurochemical research36(3)435-42(2011 Mar)

Sequences of canine glutamate decarboxylase (GAD) 1 and GAD2 genes, and variation of their genetic polymorphisms among five dog breeds.
Arata S, Hashizume C, Kikusui T, Takeuchi Y, Mori Y
The Journal of veterinary medical science70(10)1107-10(2008 Oct)

Comparison of glutamic acid decarboxylase 67 immunoreactive neurons in the hippocampal CA1 region at various age stages in dogs.
Hwang IK, Li H, Yoo KY, Choi JH, Lee CH, Chung DW, Kim DW, Seong JK, Yoon YS, Lee IS, Won MH
Neuroscience letters431(3)251-5(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that the distribution of GAD65-immunoreactive structures is different from GAD67-immunoreactive structures and that their immunoreactivity in the aged dogs is much higher than the adult dogs.
Title: Comparison of GAD65 and 67 immunoreactivity in the lumbar spinal cord between young adult and aged dogs.

GAD67 immunoreactive neurons were significantly decreased region with age in dog.
Title: Comparison of glutamic acid decarboxylase 67 immunoreactive neurons in the hippocampal CA1 region at various age stages in dogs.

The following GAD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe33651	NM_001097543.1 Latest version!	Canis lupus familiaris glutamate decarboxylase 1 (GAD1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAD1 ( NM_001097543.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001091012.1 Canis lupus familiaris glutamate decarboxylase 1 (GAD1), mRNA.

Price & Availability↑

CloneID	OCe33651
Clone ID Related Accession (Same CDS sequence)	NM_001097543.1
Accession Version	NM_001097543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Canis lupus familiaris(dog)
Product	glutamate decarboxylase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB261624.1. On May 23, 2007 this sequence version replaced XM_535958.2. ##Evidence-Data-START## Transcript exon combination :: AB261624.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA103936001, SAMEA103936002 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTCTT CGACCCCTTC TTCGTCCGCA ACCTCCTCGA ATGCGGGAGC GGACCCCAAT 
ACTGCCAACC TGCGCCCCAC AACGTATGAC ACCTGGTGCG GCGTGGCCCA TGGATGCACG 
AGAAAACTGG GTCTCAAGAT CTGCGGCTTC TTGCAAAGGA CCAACAGCCT GGAAGAGAAG 
AGCCGGCTTG TGAGCGCCTT CAAGGAGAGG CAGTCCTCCA AGAACCTGCT TTCCTGTGAA 
AACAGTGACA GGGATGGCCG TTTCCGGCGC ACAGAGACTG ATTTCTCCAA CCTCTTTGCT 
CGAGATCTGC TTCCAGCTAA GAATGGGGAG GAGCAAACAG TGCAGTTCCT CCTGGAAGTG 
GTAGACATAC TCCTCAACTA TGTCCGCAAG ACATTTGATC GCTCCACCAA GGTACTGGAC 
TTCCATCACC CGCACCAGCT GCTGGAAGGC ATGGAGGGCT TCAACTTGGA GCTGTCTGAC 
CACCCCGAGT CCCTGGAGCA GATCTTGGTT GACTGCAGAG ACACCCTGAA GTATGGGGTC 
CGTACAGGTC ATCCTCGATT TTTCAACCAG CTCTCCACTG GATTGGATAT CATTGGTTTA 
GCTGGCGAAT GGCTGACATC AACTGCCAAT ACCAATATGT TTACATATGA AATTGCACCA 
GTATTTGTCC TCATGGAGCA AATAACACTT AAGAAGATGA GAGAGATAGT TGGATGGTCA 
AGTAAAGATG GTGATGGGAT ATTTTCTCCT GGGGGAGCCA TCTCCAACAT GTACAGCATC 
ATGGCTGCTC GCTACAAGTT CTTCCCGGAA GTTAAGACAA AGGGCATGGC GGCCGTTCCC 
AAACTGGTCC TCTTCACCTC AGAACACAGT CACTATTCCA TAAAGAAGGC TGGAGCTGCA 
CTTGGCTTTG GAACTGACAA TGTGATTTTG ATAAAGTGCA ACGAAAGGGG GAAGATAATT 
CCAGCTGACT TAGAAGCAAA AATTCTTGAA GCCAAGCAAA AGGGATATGT TCCTCTTTAC 
GTCAATGCAA CTGCTGGCAC GACTGTGTAT GGGGCTTTTG ATCCGATACA GGAGATTGCA 
GATATATGTG AGAAATATAA CCTATGGCTG CATGTCGATG CTGCCTGGGG CGGTGGGCTG 
CTCATGTCCC GGAAGCACCG CCACAAACTC AGCGGCATAG AAAGGGCCAA CTCAGTCACC 
TGGAACCCTC ACAAGATGAT GGGCGTGCTG TTGCAGTGCT CTGCCATTCT TGTCAAGGAA 
AAGGGTATAC TCCAAGGATG CAACCAGATG TGTGCAGGAT ACCTTTTCCA GCCAGACAAG 
CAGTACGACG TCTCTTATGA CACTGGGGAC AAGGCAATCC AGTGTGGTCG CCACGTGGAC 
ATTTTCAAGT TCTGGCTGAT GTGGAAAGCA AAGGGCACAG TGGGATTTGA AAATCAGATC 
AACAAATGCT TGGAATTGGC CGAATACCTC TATGCCAAGA TTAAAAACAG AGAAGAATTT 
GAGATGGTTT TCGACGGTGA GCCTGAGCAT ACAAACGTCT GTTTCTGGTA TATTCCACAA 
AGCCTCAGGG GCATTCCAGA TAGCCCTGAA CGACGGGAAA AACTACACAG GGTGGCTCCC 
AAAATCAAAG CCCTGATGAT GGAGTCTGGC ACAACGATGG TTGGCTACCA GCCTCAGGGG 
GACAAGGCCA ACTTTTTCCG GATGGTCATC TCGAACCCAG CTGCTACACA GTCTGATATT 
GACTTCCTCA TTGAGGAGAT AGAAAGACTG GGCCAGGATC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091012.1
CDS	1..1785
Translation	MASSTPSSSATSSNAGADPNTANLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFKERQSSKNLLSCENSDRDGRFRRTETDFSNLFARDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIVGWSSKDGDGIFSPGGAISNMYSIMAARYKFFPEVKTKGMAAVPKLVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILEAKQKGYVPLYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFDGEPEHTNVCFWYIPQSLRGIPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL

Target ORF information:

RefSeq Version	NM_001097543.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glutamate decarboxylase 1 (GAD1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097543.1

ORF Insert Sequence:

ATGGCGTCTT CGACCCCTTC TTCGTCCGCA ACCTCCTCGA ATGCGGGAGC GGACCCCAAT 
ACTGCCAACC TGCGCCCCAC AACGTATGAC ACCTGGTGCG GCGTGGCCCA TGGATGCACG 
AGAAAACTGG GTCTCAAGAT CTGCGGCTTC TTGCAAAGGA CCAACAGCCT GGAAGAGAAG 
AGCCGGCTTG TGAGCGCCTT CAAGGAGAGG CAGTCCTCCA AGAACCTGCT TTCCTGTGAA 
AACAGTGACA GGGATGGCCG TTTCCGGCGC ACAGAGACTG ATTTCTCCAA CCTCTTTGCT 
CGAGATCTGC TTCCAGCTAA GAATGGGGAG GAGCAAACAG TGCAGTTCCT CCTGGAAGTG 
GTAGACATAC TCCTCAACTA TGTCCGCAAG ACATTTGATC GCTCCACCAA GGTACTGGAC 
TTCCATCACC CGCACCAGCT GCTGGAAGGC ATGGAGGGCT TCAACTTGGA GCTGTCTGAC 
CACCCCGAGT CCCTGGAGCA GATCTTGGTT GACTGCAGAG ACACCCTGAA GTATGGGGTC 
CGTACAGGTC ATCCTCGATT TTTCAACCAG CTCTCCACTG GATTGGATAT CATTGGTTTA 
GCTGGCGAAT GGCTGACATC AACTGCCAAT ACCAATATGT TTACATATGA AATTGCACCA 
GTATTTGTCC TCATGGAGCA AATAACACTT AAGAAGATGA GAGAGATAGT TGGATGGTCA 
AGTAAAGATG GTGATGGGAT ATTTTCTCCT GGGGGAGCCA TCTCCAACAT GTACAGCATC 
ATGGCTGCTC GCTACAAGTT CTTCCCGGAA GTTAAGACAA AGGGCATGGC GGCCGTTCCC 
AAACTGGTCC TCTTCACCTC AGAACACAGT CACTATTCCA TAAAGAAGGC TGGAGCTGCA 
CTTGGCTTTG GAACTGACAA TGTGATTTTG ATAAAGTGCA ACGAAAGGGG GAAGATAATT 
CCAGCTGACT TAGAAGCAAA AATTCTTGAA GCCAAGCAAA AGGGATATGT TCCTCTTTAC 
GTCAATGCAA CTGCTGGCAC GACTGTGTAT GGGGCTTTTG ATCCGATACA GGAGATTGCA 
GATATATGTG AGAAATATAA CCTATGGCTG CATGTCGATG CTGCCTGGGG CGGTGGGCTG 
CTCATGTCCC GGAAGCACCG CCACAAACTC AGCGGCATAG AAAGGGCCAA CTCAGTCACC 
TGGAACCCTC ACAAGATGAT GGGCGTGCTG TTGCAGTGCT CTGCCATTCT TGTCAAGGAA 
AAGGGTATAC TCCAAGGATG CAACCAGATG TGTGCAGGAT ACCTTTTCCA GCCAGACAAG 
CAGTACGACG TCTCTTATGA CACTGGGGAC AAGGCAATCC AGTGTGGTCG CCACGTGGAC 
ATTTTCAAGT TCTGGCTGAT GTGGAAAGCA AAGGGCACAG TGGGATTTGA AAATCAGATC 
AACAAATGCT TGGAATTGGC CGAATACCTC TATGCCAAGA TTAAAAACAG AGAAGAATTT 
GAGATGGTTT TCGACGGTGA GCCTGAGCAT ACAAACGTCT GTTTCTGGTA TATTCCACAA 
AGCCTCAGGG GCATTCCAGA TAGCCCTGAA CGACGGGAAA AACTACACAG GGTGGCTCCC 
AAAATCAAAG CCCTGATGAT GGAGTCTGGC ACAACGATGG TTGGCTACCA GCCTCAGGGG 
GACAAGGCCA ACTTTTTCCG GATGGTCATC TCGAACCCAG CTGCTACACA GTCTGATATT 
GACTTCCTCA TTGAGGAGAT AGAAAGACTG GGCCAGGATC TGTAA