SEMA5A cDNA ORF clone, Canis lupus familiaris(dog)

The following SEMA5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe30336 XM_005639678.3
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_022414307.1
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_022414308.1
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_022414305.1
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110324.2
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_005639673.2
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110327.2
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110325.2
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_005639674.2
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_843348.5
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_005639671.1
Latest version!
Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110326.2
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_022414306.1
Latest version!
Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OCe30336 XM_005639674.1 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110326.1 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110325.1 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110324.1 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_843348.4 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_005639673.1 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_005639678.2 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OCe30336 XM_014110327.1 Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110326.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965801.1
    CDS359..3580
    Translation

    Target ORF information:

    RefSeq Version XM_014110326.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110326.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_022414306.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270014.1
    CDS464..3685
    Translation

    Target ORF information:

    RefSeq Version XM_022414306.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414306.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639674.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639731.1
    CDS316..3537
    Translation

    Target ORF information:

    RefSeq Version XM_005639674.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639674.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_022414307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270015.1
    CDS265..3486
    Translation

    Target ORF information:

    RefSeq Version XM_022414307.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110325.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965800.1
    CDS496..3717
    Translation

    Target ORF information:

    RefSeq Version XM_014110325.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110325.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_843348.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_843348.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_848441.3
    CDS278..3499
    Translation

    Target ORF information:

    RefSeq Version XM_843348.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_843348.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639678.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005639678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639735.1
    CDS307..3528
    Translation

    Target ORF information:

    RefSeq Version XM_005639678.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639678.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639674.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639731.1
    CDS370..3591
    Translation

    Target ORF information:

    RefSeq Version XM_005639674.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110326.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965801.1
    CDS453..3674
    Translation

    Target ORF information:

    RefSeq Version XM_014110326.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110324.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110324.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965799.1
    CDS558..3779
    Translation

    Target ORF information:

    RefSeq Version XM_014110324.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110324.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110325.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110325.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965800.1
    CDS440..3661
    Translation

    Target ORF information:

    RefSeq Version XM_014110325.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110325.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639673.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639730.1
    CDS464..3685
    Translation

    Target ORF information:

    RefSeq Version XM_005639673.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639673.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110324.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965799.1
    CDS612..3833
    Translation

    Target ORF information:

    RefSeq Version XM_014110324.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110324.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_022414305.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270013.1
    CDS562..3783
    Translation

    Target ORF information:

    RefSeq Version XM_022414305.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414305.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639671.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639728.1
    CDS468..3689
    Translation

    Target ORF information:

    RefSeq Version XM_005639671.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639671.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_022414308.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270016.1
    CDS346..3567
    Translation

    Target ORF information:

    RefSeq Version XM_022414308.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414308.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_843348.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_843348.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_848441.3
    CDS224..3445
    Translation

    Target ORF information:

    RefSeq Version XM_843348.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_843348.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110327.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product semaphorin-5A
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726119.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965802.1
    CDS267..3488
    Translation

    Target ORF information:

    RefSeq Version XM_014110327.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639673.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639673.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639730.1
    CDS410..3631
    Translation

    Target ORF information:

    RefSeq Version XM_005639673.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639673.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_005639678.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005639678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005639735.1
    CDS340..3561
    Translation

    Target ORF information:

    RefSeq Version XM_005639678.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005639678.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe30336
    Clone ID Related Accession (Same CDS sequence) XM_014110326.2 , XM_022414306.1 , XM_005639674.2 , XM_022414307.1 , XM_014110325.1 , XM_843348.4 , XM_005639678.2 , XM_005639674.1 , XM_014110326.1 , XM_014110324.2 , XM_014110325.2 , XM_005639673.1 , XM_014110324.1 , XM_022414305.1 , XM_005639671.1 , XM_022414308.1 , XM_843348.5 , XM_014110327.1 , XM_005639673.2 , XM_005639678.3 , XM_014110327.2
    Accession Version XM_014110327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product semaphorin-5A
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006616.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014110327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013965802.1
    CDS151..3372
    Translation

    Target ORF information:

    RefSeq Version XM_014110327.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris semaphorin 5A (SEMA5A), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGGA CCTGCGTCAT TGCGTGGCTG TTCTCAAGCC TGGGGTGGTG GAGACTCGCC 
    CAGCCAGAAA CAGATACATC CCAGTGCCAG AGAGCGGAGC ACCCGGTCAC TTCCTATAAA
    GAAATTGGCC CCTGGTTACG GGAGTTCAGA GCGAAGAATG CTGTGGATTT CTCGCAGTTA
    ACATTTGACC CAGGACAGAA AGAACTTGTT GTAGGAGCAA GAAACTACCT CTTCAGGTTA
    CAGCTTGAGG ATTTGTCTCT GATCCAGGCT GTGGAGTGGG AGTGTGACGA AGCCACCAAG
    AAGGCCTGTT ACAGTAAGGG TAAATCCAAG GAGGAATGTC AGAATTACAT CCGGGTGCTT
    TTGGTGAGTG GTGACTGGTT ATTTACCTGT GGGACCAATG CTTTCACACC TGTCTGCACC
    AACCGCACGT TGAGTAACCT GACCGAGATC CATGATCAGA TCAGTGGCAT GGCCCGCTGT
    CCCTACAGTC CCCAGCACAA TTCCACGGCT CTTCTCACAG CCGGCGGGGA ACTCTATGCT
    GCCACAGCCA TGGATTTTCC GGGACGGGAT CCTGCCATTT ACCGGAGTCT GGGCATCTTA
    CCTCCTCTCC GCACGGCCCA GTACAACTCC AAGTGGCTCA ATGAACCAAA CTTCGTGTCA
    TCTTACGACA TTGGAAATTT CACCTACTTC TTTTTCCGAG AGAATGCCGT GGAGCACGAT
    TGTGGGAAAA CTGTGTTCTC CAGAGCTGCC CGGGTCTGTA AGAATGACAT CGGGGGCCGG
    TTCCTGCTGG AGGACACCTG GACCACATTC ATGAAGGCTC GCCTGAACTG CTCCCGGCCC
    GGGGAAGTCC CTTTTTACTA CAACGAACTG CAGAGCACTT TCTTCCTGGC CGAGCTGGAT
    TTGATCTATG GCATCTTTAC CACCAATGTG AACAGCATCG CTGCCTCAGC CGTGTGTGTC
    TTCAACCTGA GCGCCATCTC CCAGGCCTTC AACGGACCCT TCAAGTACCA GGAGAATTCA
    CGCTCTGCCT GGCTGCCGTA TCCCAACCCC AACCCCAACT TCCAGTGCGG CACGGTGGAT
    CAGGGCCTGT ACATGAACCT GACAGAGAGG AACCTGCAGG ACGCTCAGAA GTTCATTCTG
    ATGCACGAGG TGGTGCAGCC GGTGACCTCG GTCCCCTCCT TCATGGAAGA CAGCAGCCGC
    TTCTTCCACA TGGTCGTCGA TGTGGTGCAG GGTAGAGACA CGCTCATCCA CGTCATCTAT
    CTGGCCACAG GTTATGGCAC CATTAAAAAA GTGCGGGCGC CCCTGACTCC AGCTGCCGGC
    AGCTGTTTGC TGGAAGAGAT TGAGATCTTC CCCGAGCAGC GGGCAGAGCC CATAAGGAGT
    CTGCAGATCC TCCACAGCCA GAGTGCCCTG TTCGTGGGCT TGCAGGAGCA TGTGGCCAAG
    ATCCAGCTGA AGAGGTGCCA GTTCTATCGC ACCCGCAGCG CCTGCATTGG GGCCCAGGAC
    CCTTACTGTG GCTGGGACAT GGTGATGAAG AAATGCACGA GTCTGGAGGA CAGCCTGAAC
    ATGACGCAGT GGGAGCAGAT CACCTCCACC TGTCCTATAA GGAATCTCAC CGTGGACGGG
    CACTTCGGCG CGTGGTCTCC ATGGACACCC TGCACGCACA CCGATGGCAG CGCCGGGGGG
    TCCTGCCTGT GTCGCACCCG CACCTGTGAC AGCCCGGCCC CCCAGTGCGG CGGCTGGCAA
    TGTGAGGGCC CCCGCATGGA GATCGCCAAC TGTTCAAGGA ATGGAGGCTG GACCCCCTGG
    ACCTCGTGGT CTCCCTGCAG CACCACCTGT GGGATCGGCT TCCAGGTGCG CCAGCGATCC
    TGCAGCAACC CCACTCCGCG GCACGGGGGC CGGGTGTGCG TGGGCCAGAA CCGCGAGGAG
    AGATACTGCA ATGAGCATTT GCTGTGCCCC CCGCACATGT TCTGGACGGG CTGGGGTCCT
    TGGGAACGGT GCACAGCCCA GTGTGGCGGT GGCATTCAAG CTCGCCGCAG GACCTGTGAG
    AATGGACCTG ACTGCGCGGG CTGTAATGTG GAGTACCAGC CTTGCAACAC CAACCCATGC
    CCCGAGCTGA AGAAGACCAC GCCCTGGACG CCCTGGACAC CCGTCAACAT CTCAGACAAC
    GGGGGGCACT ATGAGCAGCG ATTCCGCTAC ACATGCAAAG CCCGCCTGCC TGATCCGAAT
    TTGCTGGAAG TGGGAAGGCA GAGAATCGAG ATGCGGTACT GTTCCAGCGA TGGGACCAGC
    GGCTGCTCCA CAGACGGCCT TTCTGGTGAT TTCCTGCGTG CTGGGAGGTA CTCCGCCCAC
    ACCGTCAACG GTGCTTGGTC AGCCTGGACA TCGTGGTCAC AGTGCAGCCG TGACTGTAGC
    AGGGGCATCC GGAACCGGAA GCGTGTTTGC AACAACCCTG AGCCCAAGTA TGGAGGGATG
    CCTTGCCTGG GCCCATCCCT AGAATACCAG GAATGCAACA TTTTGCCCTG CCCAGTGGAT
    GGCGTGTGGT CTTGCTGGTC TCCCTGGTCA AAATGCTCAG CGACATGTGG CGGTGGACAC
    TACATGAGGA CCCGCTCATG CACAAACCCA GCCCCAGCCT ACGGAGGAGA CATCTGCCTG
    GGGCTTCACA CGGAGGAGGC AGTCTGCAAC ACGGACCCCT GCCCGGAGAG CTGGTCAGAG
    TGGTCAGACT GGTCTGAATG TGCCGTGTCT GGGGTCCAGG TCCGTGCCCG CCAATGCATT
    CTTCTGTTCC CTGTGGGCAG TCAGTGTTCT GGAAACACCA CAGAGAGCCG CCCTTGTGTT
    TTCGATTCCA ATTTCGTCCC AGAAGTATCT GTGGCAAGAT CCAGTAGTGT AGAAGAGAAA
    AGGTGTGGGG AGTTCAATCT GTTCCACATG CTTGCTGTGG GGCTGAGCAG CTCCATTCTA
    GGCTGCCTCC TTACACTGCT CGTCTACACC TACTGCCAAC GGTACCAGCA GCAGTCCCAC
    GATGCCACCG TCATCCACCC TGTTGCTCCT GCCCCTCTCA ACACCAGCAT AACTAACCAC
    ATCAACAAAC TGGACAAGTA TGACTCGGTG GAAGCTATCA AGGCATTTAA CAAAAACAAC
    TTGATCCTAG AGGAAAGAAA CAAATACTTC AACCCGCATC TCAGTGGGAA GACCTATTCT
    AACGCCTACT TTACAGATCT CAATAATTAC GATGAGTACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.