SLC9A1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC9A1
Entrez Gene ID	478171
Full Name	solute carrier family 9 member A1
Synonyms	NHE1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287028.1	NP_001273957.1	sodium/hydrogen exchanger 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	slc9a1	XP_002934703.2
	SLC9A1	XP_001115213.2
Bos taurus (cattle)	SLC9A1	NP_777258.1
Rattus norvegicus (Norway rat)	Slc9a1	NP_036784.1
Gallus gallus (chicken)	SLC9A1	NP_001038108.1
Mus musculus (house mouse)	Slc9a1	NP_058677.1
Homo sapiens (human)	SLC9A1	NP_003038.2
Pan troglodytes (chimpanzee)	SLC9A1	XP_513239.4
Canis lupus familiaris (dog)	SLC9A1	XP_535345.2
Danio rerio (zebrafish)	slc9a1	NP_001106952.1

Gene Ontology
Bibliography

Related articles in PubMed

High glucose concentration stimulates NHE-1 activity in distal nephron cells: the role of the Mek/Erk1/2/p90RSK and p38MAPK signaling pathways.
da Costa-Pessoa JM, Damasceno RS, Machado UF, Beloto-Silva O, Oliveira-Souza M
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology33(2)333-43(2014)

Acidosis-mediated regulation of the NHE1 isoform of the Na?/H? exchanger in renal cells.
Odunewu A, Fliegel L
American journal of physiology. Renal physiology305(3)F370-81(2013 Aug)

Insulin increases acid production and may directly stimulate Na/H(+) exchange activity in cultured vascular smooth muscle cells.
Kahn AM, Yang M
Journal of vascular research48(6)505-12(2011)

Functional role of Na+-HCO3- cotransport in migration of transformed renal epithelial cells.
Schwab A, Rossmann H, Klein M, Dieterich P, Gassner B, Neff C, Stock C, Seidler U
The Journal of physiology568(Pt 2)445-58(2005 Oct)

Cell shrinkage activates Na+/H+ exchange in dog red cells by relieving inhibition of exchange by Na+ in isotonic medium.
Dunham PB
Blood cells, molecules & diseases32(3)389-93(2004 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggested that extracellular high glucose stimulated NHE-1 acutely and chronically through Mek/Erk1/2/p90(RSK) and p38MAPK pathways, respectively.
Title: High glucose concentration stimulates NHE-1 activity in distal nephron cells: the role of the Mek/Erk1/2/p90RSK and p38MAPK signaling pathways.

in canine cells activation of NHE1 occurs through an ERK-dependent pathway affecting phosphorylation of a distinct set of amino acids in the cytosolic regulatory tail of NHE1
Title: Acidosis-mediated regulation of the NHE1 isoform of the Na?/H? exchanger in renal cells.

It is concluded that insulin stimulates acid accumulation in vascular smooth muscle cells that is normally effluxed by increased Na(+)/H(+) exchange activity.
Title: Insulin increases acid production and may directly stimulate Na/H(+) exchange activity in cultured vascular smooth muscle cells.

Erythrocyte shrinkage activates NHE1 by decreasing the apparent affinity for Na+ at the inhibitory sites. Shrinkage has no effect on the apparent affinity for Na+ at the substrate sites for activation of NHE1.
Title: Cell shrinkage activates Na+/H+ exchange in dog red cells by relieving inhibition of exchange by Na+ in isotonic medium.

The following SLC9A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC9A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00449	NM_001287028.1 Latest version!	Canis lupus familiaris solute carrier family 9 member A1 (SLC9A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC9A1 ( NM_001287028.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001273957.1 Canis lupus familiaris solute carrier family 9 member A1 (SLC9A1), mRNA.

Price & Availability↑

CloneID	OCe00449
Clone ID Related Accession (Same CDS sequence)	NM_001287028.1
Accession Version	NM_001287028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-27
Organism	Canis lupus familiaris(dog)
Product	sodium/hydrogen exchanger 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03001717.1. On Nov 23, 2013 this sequence version replaced XM_535345.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGCTGCTTC TGTGGTCTGG CATCTGCCGC CGCGCTCCTC CGCGAATCTT CCCTTCGTTC 
CTGGTGGCGG TAGCCTTGGT GGGGCTGCTA CCTGTTCTCA GGAGCCATGG CCTGCAGCCC 
AGCCCTACTG CCAGCACCAT CCGAGGCTCA GAGCCACCAC GGGAACGCTC GATTGGGGAT 
GTCACCACTG CCCCACCGGA GCTGGCCCCC GAGAGCCGTC CTGTTAACCA TTCTGTCACT 
GAGCACAGCA TGAAGACTCG AAAGGCCTTC CCAGTCCTGG GCATTGACTA CACACATGTG 
CGCACACCCT TTGAGATCTC GCTTTGGATC CTGCTGGCCT GCCTCATGAA GATAGGTTTC 
CACGTGATCC CCACTATCTC GAGCATCGTC CCGGAAAGCT GCCTGCTGAT CGTAGTGGGG 
CTGCTAGTGG GGGGCCTGAT CAAGGGCGTG GGCGAGACGC CCCCCTTCCT GCAATCAGAC 
GTCTTCTTCC TTTTCCTGCT GCCACCCATC ATCCTGGACG CTGGCTACTT CCTGCCACTG 
CGGCAGTTCA CTGAGAACCT GGGCACCATC CTGATCTTCG CTGTGGTGGG CACACTGTGG 
AATGCCTTCT TCCTGGGCGG CCTCATGTAC GCCGTGTGCC TGGTGGGCGG TGAGCAGATC 
AACAACATCG GCCTCCTGGA CAACCTGCTC TTCGGCAGCA TCATCTCCGC CGTGGATCCG 
GTGGCCGTGC TGGCCGTCTT CGAGGAGATC CACATCAATG AGCTGCTGCA CATCCTTGTC 
TTTGGGGAGT CCCTGCTCAA TGACGCCGTC ACTGTGGTCC TGTATCACCT CTTTGAGGAG 
TTTGCCAACT ATGACCGAGT GGGCATCGTG GACATCGTCC TTGGCTTCCT GAGCTTCTTC 
GTAGTGTCCC TGGGCGGGGT GTTTGTAGGT GTGGTCTACG GAGTCATCGC AGCCTTCACC 
TCCCGATTCA CCGCCCACAT CCGCGTCATC GAGCCACTCT TCGTCTTCCT CTACAGCTAC 
ATGGCCTACC TGTCAGCTGA GCTCTTCCAC CTGTCGGGCA TCATGGCACT AATCGCCTCA 
GGAGTGGTGA TGCGCCCCTA CGTGGAGGCC AACATCTCCC ACAAGTCTCA TACGACCATC 
AAGTACTTCC TGAAGATGTG GAGCAGCGTC AGTGAGACCC TCATTTTCAT CTTCCTCGGC 
GTCTCCACCG TGGCCGGCTC CCACCACTGG AACTGGACCT TTGTCATCAG CACCCTGCTC 
TTCTGCCTCA TCGCCCGAGT GCTGGGTGTG CTGGGCCTGA CCTGGTTCAT CAACAAGTTC 
CGAATCGTGA AGCTGACCCC CAAGGACCAG TTCATCATTG CTTACGGGGG TCTTCGAGGG 
GCCATAGCCT TCTCCCTGGG CTACCTCCTG GACAAGAAGC ACTTCCCCAT GTGTGACCTG 
TTCCTCACTG CCATCATCAC TGTCATCTTC TTCACCGTCT TTGTGCAGGG CATGACCATT 
CGGCCCCTGG TAGACCTGTT GGCTGTGAAG AAAAAGCAAG AAACGAAGCG CTCTATCAAC 
GAGGAGATCC ACACACAGTT CCTGGACCAC CTTCTGACAG GCATCGAGGA CATCTGTGGC 
CATTACGGCC ACCACCACTG GAAGGACAAG CTCAACCGGT TTAACAAGAA ATATGTGAAG 
AAGTGTCTGA TAGCAGGGGA GCGCTCCAAG GAACCCCAGC TCATCGCCTT CTACCACAAG 
ATGGAGATGA AGCAGGCAAT TGAGCTGGTG GAGAGCGGTG GCATGGGCAA GATCCCTTCT 
GCTGTCTCCA CTGTCTCCAT GCAGAACATC CACCCCAAGT CTCTGCCTGC TGAGCGCATC 
CTGCCGGCAC TGTCCAAGGA CAAGGAGGAG GAGATCCGCA AAATCCTGAG GAACAACTTA 
CAGAAGACGA GGCAGCGGCT GCGATCCTAC AACAGACACA CACTGGTGGC AGATCCCTAC 
GAGGAGGCCT GGAACCAGAT GCTGCTCCGC AGACAGAAGG CCCGGCAGCT AGAGCAGAAG 
ATCAATAACT ACCTGACGGT GCCAGCCCAC AAGCTGGACT CACCCACCAT GTCGAGGGCC 
CGCATTGGCT CAGACCCGCT GGCCTATGAG CCGAAGGCCG ACTTGCCTGT TATCACCATC 
GACCCAGCCT CCCCGCAGTC GCCTGAGTCC GTGGACCTGG TGAATGAGGA GCTGAAGGGC 
AAAGTCTTGG GGCTGAACCG AGATCCAGCC AGAGTGGTGG AAGAGGACGA GGAGGATGAT 
GGGGGCATTG TGATGCGGAC CAAGGAGCCC TCTTCCCCAG GCACCGATGA TGTCTTCACC 
CCTGGGCCAA ATGACAGCCC CAGCTCCCAG AGGATACAGC GCTGCCTCAG TGACCCAGGT 
CCCCACCCTG AGCCTGGGGA GGGAGAGCCT TTCATCCCCA AGGGGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273957.1
CDS	805..3255
Translation	MLLLWSGICRRAPPRIFPSFLVAVALVGLLPVLRSHGLQPSPTASTIRGSEPPRERSIGDVTTAPPELAPESRPVNHSVTEHSMKTRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVPESCLLIVVGLLVGGLIKGVGETPPFLQSDVFFLFLLPPIILDAGYFLPLRQFTENLGTILIFAVVGTLWNAFFLGGLMYAVCLVGGEQINNIGLLDNLLFGSIISAVDPVAVLAVFEEIHINELLHILVFGESLLNDAVTVVLYHLFEEFANYDRVGIVDIVLGFLSFFVVSLGGVFVGVVYGVIAAFTSRFTAHIRVIEPLFVFLYSYMAYLSAELFHLSGIMALIASGVVMRPYVEANISHKSHTTIKYFLKMWSSVSETLIFIFLGVSTVAGSHHWNWTFVISTLLFCLIARVLGVLGLTWFINKFRIVKLTPKDQFIIAYGGLRGAIAFSLGYLLDKKHFPMCDLFLTAIITVIFFTVFVQGMTIRPLVDLLAVKKKQETKRSINEEIHTQFLDHLLTGIEDICGHYGHHHWKDKLNRFNKKYVKKCLIAGERSKEPQLIAFYHKMEMKQAIELVESGGMGKIPSAVSTVSMQNIHPKSLPAERILPALSKDKEEEIRKILRNNLQKTRQRLRSYNRHTLVADPYEEAWNQMLLRRQKARQLEQKINNYLTVPAHKLDSPTMSRARIGSDPLAYEPKADLPVITIDPASPQSPESVDLVNEELKGKVLGLNRDPARVVEEDEEDDGGIVMRTKEPSSPGTDDVFTPGPNDSPSSQRIQRCLSDPGPHPEPGEGEPFIPKGQ

Target ORF information:

RefSeq Version	NM_001287028.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris solute carrier family 9 member A1 (SLC9A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287028.1

ORF Insert Sequence:

ATGCTGCTTC TGTGGTCTGG CATCTGCCGC CGCGCTCCTC CGCGAATCTT CCCTTCGTTC 
CTGGTGGCGG TAGCCTTGGT GGGGCTGCTA CCTGTTCTCA GGAGCCATGG CCTGCAGCCC 
AGCCCTACTG CCAGCACCAT CCGAGGCTCA GAGCCACCAC GGGAACGCTC GATTGGGGAT 
GTCACCACTG CCCCACCGGA GCTGGCCCCC GAGAGCCGTC CTGTTAACCA TTCTGTCACT 
GAGCACAGCA TGAAGACTCG AAAGGCCTTC CCAGTCCTGG GCATTGACTA CACACATGTG 
CGCACACCCT TTGAGATCTC GCTTTGGATC CTGCTGGCCT GCCTCATGAA GATAGGTTTC 
CACGTGATCC CCACTATCTC GAGCATCGTC CCGGAAAGCT GCCTGCTGAT CGTAGTGGGG 
CTGCTAGTGG GGGGCCTGAT CAAGGGCGTG GGCGAGACGC CCCCCTTCCT GCAATCAGAC 
GTCTTCTTCC TTTTCCTGCT GCCACCCATC ATCCTGGACG CTGGCTACTT CCTGCCACTG 
CGGCAGTTCA CTGAGAACCT GGGCACCATC CTGATCTTCG CTGTGGTGGG CACACTGTGG 
AATGCCTTCT TCCTGGGCGG CCTCATGTAC GCCGTGTGCC TGGTGGGCGG TGAGCAGATC 
AACAACATCG GCCTCCTGGA CAACCTGCTC TTCGGCAGCA TCATCTCCGC CGTGGATCCG 
GTGGCCGTGC TGGCCGTCTT CGAGGAGATC CACATCAATG AGCTGCTGCA CATCCTTGTC 
TTTGGGGAGT CCCTGCTCAA TGACGCCGTC ACTGTGGTCC TGTATCACCT CTTTGAGGAG 
TTTGCCAACT ATGACCGAGT GGGCATCGTG GACATCGTCC TTGGCTTCCT GAGCTTCTTC 
GTAGTGTCCC TGGGCGGGGT GTTTGTAGGT GTGGTCTACG GAGTCATCGC AGCCTTCACC 
TCCCGATTCA CCGCCCACAT CCGCGTCATC GAGCCACTCT TCGTCTTCCT CTACAGCTAC 
ATGGCCTACC TGTCAGCTGA GCTCTTCCAC CTGTCGGGCA TCATGGCACT AATCGCCTCA 
GGAGTGGTGA TGCGCCCCTA CGTGGAGGCC AACATCTCCC ACAAGTCTCA TACGACCATC 
AAGTACTTCC TGAAGATGTG GAGCAGCGTC AGTGAGACCC TCATTTTCAT CTTCCTCGGC 
GTCTCCACCG TGGCCGGCTC CCACCACTGG AACTGGACCT TTGTCATCAG CACCCTGCTC 
TTCTGCCTCA TCGCCCGAGT GCTGGGTGTG CTGGGCCTGA CCTGGTTCAT CAACAAGTTC 
CGAATCGTGA AGCTGACCCC CAAGGACCAG TTCATCATTG CTTACGGGGG TCTTCGAGGG 
GCCATAGCCT TCTCCCTGGG CTACCTCCTG GACAAGAAGC ACTTCCCCAT GTGTGACCTG 
TTCCTCACTG CCATCATCAC TGTCATCTTC TTCACCGTCT TTGTGCAGGG CATGACCATT 
CGGCCCCTGG TAGACCTGTT GGCTGTGAAG AAAAAGCAAG AAACGAAGCG CTCTATCAAC 
GAGGAGATCC ACACACAGTT CCTGGACCAC CTTCTGACAG GCATCGAGGA CATCTGTGGC 
CATTACGGCC ACCACCACTG GAAGGACAAG CTCAACCGGT TTAACAAGAA ATATGTGAAG 
AAGTGTCTGA TAGCAGGGGA GCGCTCCAAG GAACCCCAGC TCATCGCCTT CTACCACAAG 
ATGGAGATGA AGCAGGCAAT TGAGCTGGTG GAGAGCGGTG GCATGGGCAA GATCCCTTCT 
GCTGTCTCCA CTGTCTCCAT GCAGAACATC CACCCCAAGT CTCTGCCTGC TGAGCGCATC 
CTGCCGGCAC TGTCCAAGGA CAAGGAGGAG GAGATCCGCA AAATCCTGAG GAACAACTTA 
CAGAAGACGA GGCAGCGGCT GCGATCCTAC AACAGACACA CACTGGTGGC AGATCCCTAC 
GAGGAGGCCT GGAACCAGAT GCTGCTCCGC AGACAGAAGG CCCGGCAGCT AGAGCAGAAG 
ATCAATAACT ACCTGACGGT GCCAGCCCAC AAGCTGGACT CACCCACCAT GTCGAGGGCC 
CGCATTGGCT CAGACCCGCT GGCCTATGAG CCGAAGGCCG ACTTGCCTGT TATCACCATC 
GACCCAGCCT CCCCGCAGTC GCCTGAGTCC GTGGACCTGG TGAATGAGGA GCTGAAGGGC 
AAAGTCTTGG GGCTGAACCG AGATCCAGCC AGAGTGGTGG AAGAGGACGA GGAGGATGAT 
GGGGGCATTG TGATGCGGAC CAAGGAGCCC TCTTCCCCAG GCACCGATGA TGTCTTCACC 
CCTGGGCCAA ATGACAGCCC CAGCTCCCAG AGGATACAGC GCTGCCTCAG TGACCCAGGT 
CCCCACCCTG AGCCTGGGGA GGGAGAGCCT TTCATCCCCA AGGGGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High glucose concentration stimulates NHE-1 activity in distal nephron cells: the role of the Mek/Erk1/2/p90RSK and p38MAPK signaling pathways.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology33(2)333-43(2014)
da Costa-Pessoa JM,Damasceno RS,Machado UF,Beloto-Silva O,Oliveira-Souza M

Acidosis-mediated regulation of the NHE1 isoform of the Na?/H? exchanger in renal cells.
American journal of physiology. Renal physiology305(3)F370-81(2013 Aug)
Odunewu A,Fliegel L

Insulin increases acid production and may directly stimulate Na/H(+) exchange activity in cultured vascular smooth muscle cells.
Journal of vascular research48(6)505-12(2011)
Kahn AM,Yang M

Functional role of Na+-HCO3- cotransport in migration of transformed renal epithelial cells.
The Journal of physiology568(Pt 2)445-58(2005 Oct)
Schwab A,Rossmann H,Klein M,Dieterich P,Gassner B,Neff C,Stock C,Seidler U

Cell shrinkage activates Na+/H+ exchange in dog red cells by relieving inhibition of exchange by Na+ in isotonic medium.
Blood cells, molecules & diseases32(3)389-93(2004 May-Jun)
Dunham PB

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SLC9A1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報